Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,814,336 A→G 7.0% E158G (GAA→GGA)  pheT → phenylalanine‑‑tRNA ligase subunit beta

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,814,3360AG 7.0% 166.4 / 2.7 57E158G (GAA→GGA) pheTphenylalanine‑‑tRNA ligase subunit beta
Reads supporting (aligned to +/- strand):  ref base A (28/25);  new base G (2/2);  total (30/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.51e-01

CGCGGTGTCGAGTCCTTCGGCATGCTGTGCTCGGCTGCCGAGCTGCAGATCAGCGAAGAAAACGACGGCCTGCTGGAGCTGGCTGCCGACGCTCCGGTTGGCGAAGACATTCGCACGTACCTGAGCCTGGATGATGCCAGCATCGAAATCGGCCTGACCCCGAACCGCGGCGACTGCCTGTCCATCGCCGGCCTGGCCCGCGACGTCAGCGCCCTGTACGACACCCCGGTCACCCGCCCGGTGGTGCCAGCCGTAGCGGCCGCCCATGACGAAGTGCGCCCGGTCGAA  >  NC_002947/2814191‑2814478
                                                                                                                                                 |                                                                                                                                              
cggggTGGCGAGTCCTTCGGCATGCTGTGCTCGGCTGCCGAGCTGCAGATCAGCGAAGAAAACGACGGCCTGCTGGAGCTGGCTGCCGACGCTCCGGTTGGCGAAGACATTCGCACGTACCTGAGCCTGGATGATGCCAGCATCGAAAt                                                                                                                                             <  6:250194/146‑1 (MQ=255)
   ggTGTCGAGTCCTTCGGCATGCTGTGCTCGGCTGCCGAGCTGCAGATCAGCGAAGAAAACGACGGCCTGCTGGAGCTGGCTGCCGACGCTCCGGTTGGCGAAGACATTCGCACGTACCTGAGCCTGGATGATGCCAGCATCGAAATCgg                                                                                                                                          >  4:268633/1‑149 (MQ=255)
       tCGAGTCCTTCGGCATGCTGTGCTCGGCTGCCGAGCTGCAGATCAGCGAAGAAAACGACGGCCTGCTGGAGCTGGCTGCCGACGCTCCGGTTGGCGAAGACATTCGCACGTACCTGAGCCTGGATGATGCCAGCATCGAAATCGGCCt                                                                                                                                       <  2:71613/148‑1 (MQ=255)
         gAGTCCTTCGGCATGCTGTGCTCGGCTGCCGAGCTGCAGATCAGCGAAGAAAACGACGGCCTGCTGGAGCTGGCTGCCGACGCTCCGGTTGGCGAAGACATTCGCACGTACCTGAGCCTGGATGATGCCAGCATCGAAATCGGCCTGAc                                                                                                                                    >  1:305931/1‑149 (MQ=255)
          aGTCCTTCGGCATGCTGTGCTCGGCTGCCGAGCTGCAGATCAGCGAAGAAAACGACGGCCTGCTGGAGCTGGCTGCCGACGCTCCGGTTGGCGAAGACATTCGCACGTACCTGAGCCTGGATGATGCCAGCATCGAAATCGGCCTGAcc                                                                                                                                   >  3:189790/1‑149 (MQ=255)
          aGTCCTTCGGCATGCTGTGCTCGGCTGCCGAGCTGCAGATCAGCGAAGAAAACGACGGCCTGCTGGAGCTGGCTGCCGACGCTCCGGTTGGCGAAGACATTCGCACGTACCTGAGCCTGGATGATGCCAGCATCGAAATCGGCCTGAcc                                                                                                                                   >  3:144464/1‑149 (MQ=255)
              cTTCGGCATGCTGTGCTCGGCTGCCGAGCTGCAGATCAGCGAAGAAAACGACGGCCTGCTGGAGCTGGCTGCCGACGCTCCGGTTGGCGAAGACATTCGCACGTACCTGAGCCTGGATGATGCCAGCATCGAAATCGGCCTGACCCCg                                                                                                                                >  6:44157/1‑148 (MQ=255)
                  ggCATGCTGTGCTCGGCTGCCGAGCTGCAGATCAGCGAAGAAAACGACGGCCTGCTGGAGCTGGCTGCCGACGCTCCGGTTGGCGAAGACATTCGCACGTACCTGAGCCTGGATGATGCCAGCATCGAAATCGGCCTGACCCCGAAcc                                                                                                                            <  5:44157/148‑1 (MQ=255)
                             cTCGGCTGCCGAGCTGCAGATCAGCGAAGAAAACGACGGCCTGCTGGAGCTGGCTGCCGACGCTCCGGTTGGCGAAGACATTCGCACGTACCTGAGCCTGGATGATGCCAGCATCGAAATCGGCCTGACCCCGAACCGCGGCGACTGc                                                                                                                 <  8:266512/148‑1 (MQ=255)
                              tCGGCTGCCGAGCTGCAGATCAGCGAAGAAAACGACGGCCTGCTGGAGCTGGCTGCCGACGCTCCGGTTGGCGAAGACATTCGCACGTACCTGAGCCTGGATGATGCCAGCATCGAAATCGGCCTGACCCCGAACCGCGGCGACTGCCt                                                                                                               >  3:47940/1‑149 (MQ=255)
                               cGGCTGCCGAGCTGCAGATCAGCGAAGAAAACGACGGCCTGCTGGAGCTGGCTGCCGACGCTCCGGTTGGCGAAGACATTCGCACGTACCTGAGCCTGGATGATGCCAGCATCGAAATCGGc                                                                                                                                         <  6:232593/122‑1 (MQ=255)
                               cGGCTGCCGAGCTGCAGATCAGCGAAGAAAACGACGGCCTGCTGGAGCTGGCTGCCGACGCTCCGGTTGGCGAAGACATTCGCACGTACCTGAGCCTGGATGATGCCAGCATCGAAATCGGc                                                                                                                                         >  5:232593/1‑122 (MQ=255)
                                ggCTGCCGAGCTGCAGATCAGCGAAGAAAACGACGGCCTGCTGGAGCTGGCTGCCGACGCTCCGGTTGGCGAAGACATTCGCACGTACCTGAGCCTGGATGATGCCAGCATCGAAATCGGCCTGACCCCGAACCGCGGCGACTGCCTGt                                                                                                             <  4:137612/149‑1 (MQ=255)
                                     ccGAGCTGCAGATCAGCGAAGAAAACGACGGCCTGCTGGAGCTGGCTGCCGACGCTCCGGTTGGCGAAGACATTCGCACGTACCTGAGCCTGGATGATGCCAGCATCGAAATCGGCCTGACCCCGAACCGCGGCGACTGCCTGTCCATc                                                                                                        >  1:315810/1‑149 (MQ=255)
                                        aGCTGCAGATCAGCGAAGAAAACGACGGCCTGCTGGAGCTGGCTGCCGACGCTCCGGTTGGCGAAGACATTCGCACGTACCTGAGCCTGGATTATGCCAGCATCGAAATCGGCCTGACCCCGAACCGCGGCGAc                                                                                                                    >  2:59463/1‑134 (MQ=255)
                                        aGCTGCAGATCAGCGAAGAAAACGACGGCCTGCTGGAGCTGGCTGCCGACGCTCCGGTTGGCGAAGACATTCGCACGTACCTGAGCCTGGATTATGCCAGCATCGAAATCGGCCTGACCCCGAACCGCGGCGAc                                                                                                                    <  1:59463/134‑1 (MQ=255)
                                                      gaagaaAACGACGGCCTGCTGGAGCTGGCTGCCGACGCTCCGGTTGGCGAAGACATTCGCACGTACCTGAGCCTGGATGATGCCAGCATCGAAATCGGCCTGACCCCGAACCGCGGCGACTGCCTGTCCATCGCCGGCCTGGCCCGCGa                                                                                       >  5:182136/1‑149 (MQ=255)
                                                       aagaaAACGACGGCCTGCTGGAGCTGGCTGCCGACGCTCCGGTTGGCGAAGACATTCGCACGTACCTGAGCCTGGATGATGCCAGCATCGAAATCGGCCTGACCCCGAACCGCGGCGACTGCCTGTCCATCGcc                                                                                                     <  2:16505/134‑1 (MQ=255)
                                                       aagaaAACGACGGCCTGCTGGAGCTGGCTGCCGACGCTCCGGTTGGCGAAGACATTCGCACGTACCTGAGCCTGGATGATGCCAGCATCGAAATCGGCCTGACCCCGAACCGCGGCGACTGCCTGTCCATCGcc                                                                                                     >  1:16505/1‑134 (MQ=255)
                                                               gacgGCCTGCTGGAGCTGGCTGCCGACGCTCCGGTTGGCGAAGACATTCGCACGTACCTGAGCCTGGATGATGCCAGCATCGAAATCGGCCTGACCCCGAACCGCGGCGACTGCCTGTCCATCGCCGGCCTGGCCCGCGAc                                                                                      <  8:41103/141‑1 (MQ=255)
                                                               gacgGCCTGCTGGAGCTGGCTGCCGACGCTCCGGTTGGCGAAGACATTCGCACGTACCTGAGCCTGGATGATGCCAGCATCGAAATCGGCCTGACCCCGAACCGCGGCGACTGCCTGTCCATCGCCGGCCTGGCCCGCGAc                                                                                      >  7:41103/1‑141 (MQ=255)
                                                               gacgGCCTGCTGGAGCTGGCTGCCGACGCTCCGGTTGGCGAAGACATTCGCACGTACCTGAGCCTGGATGATGCCAGCATCGAAATCGGCCTGACCCCGAACCGCGGCGACTGCCTGTCCATCGCCGGCCTGGCCCGCGACGTCAgcgc                                                                              >  1:194078/1‑149 (MQ=255)
                                                                     ctgctgGAGCTGGCTGCCGACGCTCCGGTTGGCGAAGACATTCGCACGTACCTGAGCCTGGATGATGCCAGCATCGAAATCGGCCTGACCCCGAACCGCGGCGACTGCCTGTCCATCGCCGGCCTGGCCCGCGACGTCAGCGCCCTGTa                                                                        <  2:305931/149‑1 (MQ=255)
                                                                      tgctgGAGCTGGCTGCCGACGCTCCGGTTGGCGAAGACATTCGCACGTACCTGAGCCTGGATGATGCCAGCATCGAAATCGGCCTGACCCCGAACCGCGGCGACTGCCTGTCCATCGCCGGCCTGGCCCGCGACGTCAGCGCCCTGTAc                                                                       >  5:70860/1‑149 (MQ=255)
                                                                                ggctgCCGACGCTCCGGTTGGCGAAGACATTCGCACGTACCTGAGCCTGGATGATGCCAGCATCGAAATCGGCCTGACCCCGAACCGCGGCGACTGCCTGTCCATCGCCGGCCTGGCCCGCGACGTCAGCGCCCTGTACGACACCCCgg                                                             >  3:285496/1‑149 (MQ=255)
                                                                                 gctgCCGACGCTCCGGTTGGCGAAGACATTCGCACGTACCTGAGCCTGGATGATGCCAGCATCGAAATCGGCCTGACCCCGAACCGCGGCGACTGCCTGTCCATCGCCGGCCTGGCCCGCGACGTCAGCGCCCTGTACGACACCCCg                                                              <  6:182136/147‑1 (MQ=255)
                                                                                   tgCCGACGCTCCGGTTGGCGAAGACATTCGCACGTACCTGAGCCTGGATGATGCCAGCATCGGAATCGGCCTGACCCCGAACCGCGGCGACTGCCTGTCCATCGCCGGCCTGGCCCGCGACGTCAGCGCCCTGTACGACACCCCGGTCa                                                          >  6:37009/1‑149 (MQ=255)
                                                                                   tgCCGACGCTCCGGTTGGCGAAGACATTCGCACGTACCTGAGCCTGGATGATGCCAGCATCGAAATCGGCCTGACCCCGAACCGCGGCGACTGCCTGTCCATCGCCGGCCTGGCCCGCGACGTCAGCGCCCTGTACGa                                                                     <  7:297489/138‑1 (MQ=255)
                                                                                   tgCCGACGCTCCGGTTGGCGAAGACATTCGCACGTACCTGAGCCTGGATGATGCCAGCATCGAAATCGGCCTGACCCCGAACCGCGGCGACTGCCTGTCCATCGCCGGCCTGGCCCGCGACGTCAGCGCCCTGTACGa                                                                     >  8:297489/1‑138 (MQ=255)
                                                                                   tgCCGACGCTCCGGTTGGCGAAGACATTCGCACGTACCTGAGCCTGGATGATGCCAGCATCGAAATCGGCCTGACCCCGAACCGCGGCGACTGCCTGTCCATCGCCGGCCTGGCCCGCGACGTCAGCGCCCTGTACGACAccaccgggc                                                          >  5:143730/1‑142 (MQ=255)
                                                                                      cGACGCTCCGGTTGGCGAAGACATTCGCACGTACCTGAGCCTGGATGATGCCAGCATCGAAATCGGCCTGACCCCGAACCGCGGCGACTGCCTGTCCATCGCCGGCCTGGCCCGCGACGTCAGCGCCCTGTACGACACCCCGGTCAccc                                                       >  5:215892/1‑149 (MQ=255)
                                                                                                gTTGGCGAAGACATTCGCACGTACCTGAGCCTGGATGATGCCAGCATCGAAATCGGCCTGACCCCGAACCGCGGCGACTGCCTGTCCATCGCCGGCCTGGCCCGCGACGTCAGCGCCCTGTACGACACCCCGGTCACCCGCCCggtggt                                             <  6:70860/149‑1 (MQ=255)
                                                                                                cttGGCGAAGACATTCGCACGTACCAGAGCCTGCATCCTGCCAGCATCGAACTCCTCCTGACCCCGAACCGCGGCGACTGCCTGTCCATCGCCGGCCTGGCCCGCGACGTCAGCGCCCTGTACGACACCCCGGTCACCCGCCCggtggt                                             <  8:273729/148‑1 (MQ=255)
                                                                                                    gCGAAGACATTCGCACGTACCTGAGCCTGGATGATGCCAGCATCGAAATCGGCCTGACCCCGAACCGCGGCGACTGCCTGTCCATCGCCGGCCTGGCCCGCGACGTCAGCGCCCTGTACGACACCCCGGTCACCCGCCCGGTGGTGCCa                                         >  8:150328/1‑149 (MQ=255)
                                                                                                      gAAGACATTCGCACGTACCTGAGCCTGGATGATGCCAGCATCGAAATCGGCCTGACCCCGAACCGCGGCGACTGCCTGTCCATCGCCGGCCTGGCCCGCGACGTCAGCGCCCTGTACGACACCCCGGTCACCCGCCCGGTGGTGCCAGc                                       >  1:41235/1‑149 (MQ=255)
                                                                                                           cATTCGCACGTACCTGAGCCTGGATGATGCCAGCATCGAAATCGGCCTGACCCCGAACCGCGGCGACTGCCTGTCCATCGCCGGCCTGGCCCGCGACGTCAGCGCCCTGTACGACACCCCGGTCACCCGCCCGGTGGTGCCAGCCGTAg                                  <  3:268633/149‑1 (MQ=255)
                                                                                                            aTTCGCACGTACCTGAGCCTGGATGATGCCAGCATCGGAATCGGCCTGACCCCGAACCGCGGCGACTGCCTGTCCATCGCCGGCCTGGCCCGCGACGTCAGCGCCCTGTACGACACCCCGGTCACCCGCCCGGTGGTGCCAGCCGTAGc                                 <  5:37009/149‑1 (MQ=255)
                                                                                                            aTTCGCACGTACCTGAGCCTGGATGATGCCAGCATCGAAATCGGCCTGACCCCGAACCGCGGCGACTGCCTGTCCATCGCCGGCCTGGCCCGCGACGTCAGCGCCCTGTACGACACCCCGGTCACCCGCCCGGTGGTGCCAGCCGTAGc                                 >  4:69863/1‑149 (MQ=255)
                                                                                                                  aCGTACCTGAGCCTGGATGATGCCAGCATCGAAATCGGCCTGACCCCGAACCGCGGCGACTGCCTGTCCATCGCCGGCCTGGCCCGCGACGTCAGCGCCCTGTACGACACCCCGGTCACCCGCCCGGTGGTGCCAGCCGTAGCGgccgc                           <  2:41235/149‑1 (MQ=255)
                                                                                                                  aCGTACCTGAGCCTGGATGATGCCAGCATCGAAATCGGCCTGACCCCGAACCGCGGCGACTGCCTGTCCATCGCCGGCCTGGCCCGCGACGTCAGCGCCCTGTACGACACCCCGGTCACCCGCCCGGTGGTGCCAGCCGTAGCGgccgc                           <  7:150328/149‑1 (MQ=255)
                                                                                                                       ccTGAGCCTGGATGATGCCAGCATCGAAATCGGCCTGACCCCGAACCGCGGCGACTGCCTGTCCATCGCCGGCCTGGCCCGCGACGTCAGCGCCCTGTACGACACCCCGGTCACCCGCCCGGTGGTGCCAGCCGTAGCGGCCGCCCATg                      <  2:315810/149‑1 (MQ=255)
                                                                                                                        cTGAGCCTGGATGATGCCAGCATCGAAATCGGCCTGACCCCGAACCGCGGCGACTGCCTGTCCATCGCCGGCCTGGCCCGCGACGTCAGCGCCCTGTACGACACCCCGGTCACCCGCCCGGTGGTGCCAGCCGTAGCGGCCGCCCATGa                     <  4:189790/149‑1 (MQ=255)
                                                                                                                         tGAGCCTGGATGATGCCAGCATCGAAATCGGCCTGACCCCCAACCGCGGCGACAGCCAGTCCATCACCGGCCTGGCCCGCGACCTCAGCGCACAGTACGAAACACCGGTCACCCGCCCGCTGGTGCAAGCCGAAGCGGACGCCCCTGAc                    >  4:42291/1‑149 (MQ=255)
                                                                                                                          gAGCCTGGATGATGCCAGCATCGGAATCGGCCTGACCCCGAACCGCGGCGACTGCCTGTCCATCGCCGGCCTGGCCCGCGACGTCAGCGCCCTGTACGACACCCCGGTCACCCGCCCGGTGGTGCCAGCCGTAGCGGCCGCCCATGAc                    >  1:129974/1‑148 (MQ=255)
                                                                                                                           aGCCTGGATGATGCCAGCATCGAAATCGGCCTGACCCCGAACCGCGGCGACTGCCTGTCCATCGCCGGCCTGGCCCGCGACGTCAGCGCCCTGTACGACACCCCGGTCACCCGCCCGGTGGTGCCAGCCGTAGCGGCCGCCCATGACGa                  <  4:144464/149‑1 (MQ=255)
                                                                                                                            gCCTGGATGATGCCAGCATCGAAATCGGCCTGACCCCGAACCGCGGCGACTGCCTGTCCATCGCCGGCCTGGCCCGCGACGTCAGCGCCCTGTACGACACCCCGGTCACCCGCCCGGTGGTGCCAGCCGTAGCGGCCGCCCATGACGaa                 <  7:169495/149‑1 (MQ=255)
                                                                                                                                 gatgatGCCAGCATCGAAATCGGCCTGACCCCGAACCGCGGCGACTGCCTGTCCATCGCCGGCCTGGCCCGCGACGTCAGCGCCCTGTACGACACCCCGGTCACCCGCCCGGTGGTGCCAGCCGTAGCGGCCGCCCATGACGAAGTgcg            >  7:203523/1‑149 (MQ=255)
                                                                                                                                    gatgCCAGCATCGAAATCGGCCTGACCCCGAACCGCGGCGACTGCCTGTCCATCGCCGGCCTGGCCCGCGACGTCAGCGCCCTGTACGACACCCCGGTCACCCGCCCGGTGGTGCCAGCCGTAGCGGCCGCCCATGACGAAGTGCGccc         >  2:314773/1‑149 (MQ=255)
                                                                                                                                    gatgCCAGCATCGAAATCGGCCTGACCCCGAACCGCGGCGACTGCCTGTCCATCGCCGGCCTGGCCCGCGACGTCAGCGCCCTGTACGACACCCCGGTCACCCGCCCGGTGGTGCCAGCCGTAGCGGCCGCCCATGACGAAGTGCGcc          <  4:273976/148‑1 (MQ=255)
                                                                                                                                    gatgCCAGCATCGAAATCGGCCTGACCCCGAACCGCGGCGACTGCCTGTCCATCGCCGGCCTGGCCCGCGACGTCAGCGCCCTGTACGACACCCCGGTCACCCGCCCGGTGGTGCCAGCCGTAGCGGCCGCCCATGACGAAGTGCGcc          >  3:273976/1‑148 (MQ=255)
                                                                                                                                      tgCCAGCATCGGAATCGGCCTGACCCCGAACCGCGGCGACTGCCTGTCCATCGCCGGCCTGGCCCGCGACGTCAGCGCCCTGTACGACACCCCGGTCACCCGCCCGGTGGTGCCAGCCGTAGCGGCCGCCCATGACGAAGTGCGCCCgg       <  2:129974/149‑1 (MQ=255)
                                                                                                                                         cAGCATCGAAATCGGCCTGACCCCGAACCGCGGCGACTGCCTGTCCATCGCCGGCCTGGCCCGCGACGTCAGCGCCCTGTACGACACCCCGGTCACCCGCCCGGTGGTGCCAGCCGTAGCGgccgc                           <  7:234993/126‑1 (MQ=255)
                                                                                                                                         cAGCATCGAAATCGGCCTGACCCCGAACCGCGGCGACTGCCTGTCCATCGCCGGCCTGGCCCGCGACGTCAGCGCCCTGTACGACACCCCGGTCACCCGCCCGGTGGTGCCAGCCGTAGCGgccgc                           >  8:234993/1‑126 (MQ=255)
                                                                                                                                         cAGCATCGAAATCGGCCTGACCCCGAACCGCGGCGACTGCCTGTCCATCGCCGGCCTGGCCCGCGACGTCAGCGCCCTGTACGACACCCCGGTCACCCGCCCGGTGGTGCCAGCCGTAGCGGCCGCCCATGACGAAGTGCGCCCGGTc     >  5:526/1‑148 (MQ=255)
                                                                                                                                           gCATCGAAATCGGCCTGACCCCGAACCGCGGCGACTGCCTGTCCATCGCCGGCCTGGCCCGCGACGTCAGCGCCCTGTACGACACCCCGGTCACCCGCCCGGTGGTGCCAGCCGTAGCGGCCGCCCATGACGAAGTGCGCCCGGTCGaa  <  4:47940/149‑1 (MQ=255)
                                                                                                                                            cATCGAAATCGGCCTGACCCCGAACCGCGGCGACTGCCTGTCCATCGCCGGCCTGGCCCGCGACGTCAGCGCCCTGTACGACACCCCGGTCAc                                                         <  6:40723/93‑1 (MQ=255)
                                                                                                                                            cATCGAAATCGGCCTGACCCCGAACCGCGGCGACTGCCTGTCCATCGCCGGCCTGGCCCGCGACGTCAGCGCCCTGTACGACACCCCGGTCAc                                                         >  5:40723/1‑93 (MQ=255)
                                                                                                                                                 |                                                                                                                                              
CGCGGTGTCGAGTCCTTCGGCATGCTGTGCTCGGCTGCCGAGCTGCAGATCAGCGAAGAAAACGACGGCCTGCTGGAGCTGGCTGCCGACGCTCCGGTTGGCGAAGACATTCGCACGTACCTGAGCCTGGATGATGCCAGCATCGAAATCGGCCTGACCCCGAACCGCGGCGACTGCCTGTCCATCGCCGGCCTGGCCCGCGACGTCAGCGCCCTGTACGACACCCCGGTCACCCGCCCGGTGGTGCCAGCCGTAGCGGCCGCCCATGACGAAGTGCGCCCGGTCGAA  >  NC_002947/2814191‑2814478

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑