Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,844,678 A→T 6.4% intergenic (‑38/+42) PP_2495 ← / ← PP_2496 hypothetical protein/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,844,6780AT 6.4% 186.4 / 2.8 62intergenic (‑38/+42)PP_2495/PP_2496hypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (26/32);  new base T (2/2);  total (28/34)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.26e-01

CTTGCGGATCTGCAGCCAGTCCTCGCGGGGTAGCTCCTTGGTGCTAACCAGGCGCAGAGCGGGACGGATTTTGCTGGTGCTGCGATTCAATGAAGTGGCTTTCATAGTGTTCACCTTGCAGAAATAGAAACGCCCGACCTGCAACGAAGCGGGTCGGGCGTTATGGGGTGGGATTGGTAAAAGGTCAGGCGACCAATTGCAGGGCAGTATCCAAGGCGCGTTGCTTGATTTGGGCACCCTGGCCGAACCAGGCGGAGTCGAGGCGGTATTCGGTGCTGCGTGCCCGC  >  NC_002947/2844536‑2844822
                                                                                                                                              |                                                                                                                                                
cttgcGGATCTGCAGCCAGTCCTCGCGGGGTAGCTCATTGGTGCTAACCAGGCGCAGAGCGGGACGGATTTTGCTGGTGCTGCGATTCAATGAAGTGTCTTTCATAGTGTTCACCTTGCAGAAATAGAAACGCCCGACCTGCAACGaa                                                                                                                                             <  3:210343/148‑1 (MQ=255)
   gcGTATCGGCAGCCAGGCCGCGCGGGGTAGCTCCTTGGTGCTCACCAGGCGCAGAGCGGGACGGATTTTGCTGGTGCTGCGATTCAATGAAGTGGCTTTCATAGTGTGCACCTTGCAGAAATAGAAACGCCCGACCTGCAACGAAGc                                                                                                                                           <  8:275144/147‑1 (MQ=255)
      gATCTGCAGCCAGTCCTCGCGGGGTAGCTCCTTGGTGCTAACCAGGCGCAGAGCGGGACGGATTTTGCTGGTGCTGCGATTCAATGAAGTGGCTTTCATAGTGTTCACCTTGCAGAAATAGAAACGCCCGACCTGCAACGAAGCGGGTc                                                                                                                                      <  4:147475/149‑1 (MQ=255)
         cTGCAGCCAGTCCTCGCGGGGTAGCTCCTTGGTGCTAACCAGGCGCAGAGCGGGACGGATTTTGCTGGTGCTGCGATTCAATGAAGTGGCTTTCATAGTGTTCACCTTGCAGAAATAGAAACGCCCGACCTGCAACGAAGCGGGTCggg                                                                                                                                   >  5:64987/1‑149 (MQ=255)
           gCAGCCAGTCCTCGCGGGGTAGCTCCTTGGTGCTAACCAGGCGCAGAGCGGGACGGATTTTGCTGGTGCTGCGATTCAATGAAGTGGCTTTCATAGTGTTCACCTTGCAGAAATAGAAACGCCCGACCTGCAACGAAGCGGGTCGGGCg                                                                                                                                 >  4:66312/1‑149 (MQ=255)
            cAGCCAGTCCTCGCGGGGTAGCTCCTTGGTGCTAACCAGGCGCAGAGCGGGACGGATTTTGCTGGTGCTGCGATTCAATGAAGTGGCTTTCATAGTGTTCACCTTGCAGATATAGAAACGCCCGACCCGCTTCGTTGCAGGTCgg                                                                                                                                    <  1:46945/145‑1 (MQ=255)
            cAGCCAGTCCTCGCGGGGTAGCTCCTTGGTGCTAACCAGGCGCAGAGCGGGACGGATTTTGCTGGTGCTGCGATTCAATGAAGTGGCTTTCATAGTGTTCACCTTGCAGAAATAGAAACGCCCGACCTGCAACGAAGCGGGTCgg                                                                                                                                    <  1:282200/145‑1 (MQ=255)
              gCCAGTCCTCGCGGGGTAGCTCCTTGGTGCTAACCAGGCGCAGAGCGGGACGGATTTTGCTGGTGCTGCGATTCAATGAAGTGGCTTTCATAGTGTTCACCTTGCAGAAATAGAAACGCCCGACCTGCAACGAAGCGGGTCGGGCGTTa                                                                                                                              >  7:314621/1‑149 (MQ=255)
               ccAGTCCTCGCGGGGTAGCTCCTTGGTGCTAACCAGGCGCAGAGCGGGACGGATTTTGCTGGTGCTGCGATTCAATGAAGTGGCTTTCATAGTGTTCACCTTGCAGAAATAGAAACGCCCGACCTGCAACGAAGCGGGTCTGGCGTTAt                                                                                                                             >  6:202923/1‑149 (MQ=255)
                cAGTCCTCGCGGGGTAGCTCCTTGGTGCTAACCAGGCGCAGAGCGGGACGGATTTTGCTGGTGCTGCGATTCAATGAAGTGGCTTTCATAGTGTTCACCTTGCAGAAATAGAAACGCCCGACCCGCTTCGTTGCAGGTCGGGCGtttct                                                                                                                            <  3:59953/149‑4 (MQ=38)
                     cTCGCGGGGTAGCTCCTTGGTGCTAACCAGGCGCAGAGCGGGACGGATTTTGCTGGTGCTGCGATTCAATGAAGTGGCTTTCATAGTGTTCACCTTGCAGAAATAGAAACGCCCGCCCTGCAACGAAGCGGGTCGGGCGTTATGGGGTg                                                                                                                       >  4:112221/1‑149 (MQ=255)
                      tCGCGGGGTAGCTCCTTGGTGCTAACCAGGCGCAGAGCGGGACGGATTTTGCTGGTGCTGCGATTCAATGAAGTGGCTTTCATAGTGTTCACCTTGCAGAAATAGAAACGCCCGACCTGCAACGAAGCGGGTCGGGCGTTATGGGGTgg                                                                                                                      >  2:201740/1‑149 (MQ=255)
                           gggTAGCTCCTTGGTGCTAACCAGGCGCAGAGCGGGACGGATTTTGCTGGTGCTGCGATTCAATGAAGTGGCTTTCATAGTGTTCACCTTGCAGAAATAGAAACGCCCGACCTGCAACGAAGCGGGTCGGGCGTTATGGGGTGGGATTg                                                                                                                 <  3:66312/149‑1 (MQ=255)
                             gTAGCTCCTTGGTGCTAACCAGGCGCAGAGCGGGACGGATTTTGCTGGTGCTGCGATTCAATGAAGTGGCTTTCATAGTGTTCACCTTGCAGAAATAGAAACGCCCGACCTGCAACGAAGCGGGTCGGGCGTTATGGGGTGGGATTGGt                                                                                                               <  3:31926/149‑1 (MQ=255)
                                 cTCCTTGGTGCTAACCAGGCGCTGAGCGGGACGTATTTTGCTGGTGCTGCGATTCAATGAAGTGGCTTTCATAGTGTTCACCTTGCAGAAATAGAAACGCCCGACCTGCAACGAAGCGGGTCGGGCGTTATGGGGTGGGATTGGTaaaa                                                                                                           <  1:99891/149‑1 (MQ=255)
                                 cTCCTTGGTGCTAACCAGGCGCAGAGCGGGACGGATTTTGCTGGTGCTGCGATTCAATGAAGTGGCTTTCATAGTGTTCACCTTGCAGAAATAGAAACGCCCGACCTGCAACGAAGCGGGTCGGGCGTTATGGGGTGGGATTGGTaa                                                                                                             >  7:82741/1‑147 (MQ=255)
                                    cTTGGTGCTAACCAGGCGCCGAGCGGGACGGATTTTGCTGGTGCTGGGATTCAATGAAGTGGCTTTCATAGTGTTCACCTTGCAGAATTAGAAACGCCCGACCTGCAACGATGCGGGTCGGGCGTTATGGGGTGGGATTGGTAAAAGGt                                                                                                        <  4:352/149‑1 (MQ=255)
                                    cTTGGTGCTAACCAGGCGCAGAGCGGGACGGATTTTGCTGGTGCTGCGATTCAATGAAGTGGCTTTCATAGTGTTCACCTTGCAGAAATAGAAACGCCCGACCTGCAACGAAGCGGGTCGGGCGTTATGGGGTGGGATTGGTAAAAgg                                                                                                         >  1:101328/1‑148 (MQ=255)
                                         tGCTAACCAGGCGCAGAGCGGGACGGATTTTGCTGGTGCTGCGATTCAATGAAGTGGCTTTCATAGTGTTCACCTTGCAGAAATAGAAACGCCCGACCTGCAACGAAGCGGGTCGGGCGTTATGGGGTGGGATTGGTAAAAGGTc                                                                                                       <  4:260189/145‑1 (MQ=255)
                                         tGCTAACCAGGCGCAGAGCGGGACGGATTTTGCTGGTGCTGCGATTCAATGAAGTGGCTTTCATAGTGTTCACCTTGCAGAAATAGAAACGCCCGACCTGCAACGAAGCGGGTCGGGCGTTATGGGGTGGGATTGGTAAAAGGTc                                                                                                       >  3:260189/1‑145 (MQ=255)
                                         tGCTAACCAGGCGCAGAGCGGGACGGATTTTGCTGGTGCTGCGATTCAATGAAGTGGCTTTCATAGTGTTCACCTTGCAGAAATAGAAACGCCCGACCTGCAACGAAGCGGGTCGGGCGTTATGGGGTGGGATTGGTAAAAGGTCAGGc                                                                                                   <  5:242989/149‑1 (MQ=255)
                                               ccAGGCGCAGTGCGGGACGGTTTTTGCTGGTGATGCGATTCAATGATGTGGCTTTCATAGTGTTCACCTTGCAGAAATAGAAACGCCCGACCTGCATCGAAGCGGGTCGGGGGTTATGGGGTg                                                                                                                       <  6:189549/123‑1 (MQ=255)
                                               ccAGGCGCAGAGCGGGACGGATTTTGCTCGTGCTGCGATTCAATGAAGTGGCTTTCATAGTGATCACCTTCCAGAAATAGAAACGACCGACCTGCAACGAAGCGGGTCCGGCGTTATGGGGtt                                                                                                                       >  5:189549/1‑122 (MQ=255)
                                                    cgcAGAGCGGGACGGATTTTGCTGGTGCTGCGATTCAATGAAGTGGCTTTCATAGTGTTCACCTTGCAGAAATAGAAACGCCCGACCTGCAACGAAGCGGGTCGGGCGTTATGGGGTGGCATTGGTAAAAGGTCAGGCGACCAATTGCa                                                                                        <  3:112221/149‑1 (MQ=255)
                                                      cAGAGCGGGACGGATTTTGCTGGTGCTGCGATTCAATGAAGTGGCTTTCATAGTGTTCACCTTGCAGAAATAGAAACGCCCGACCTGCAACGAAGCGGGTCGGGCGTTATGGGGTGGGATTGGTAAAAGGTCAGGCGACCAATTGCAgg                                                                                      <  1:40982/149‑1 (MQ=255)
                                                       agagCGGGACGGATTTTGCTGGTGCTGCGATTCAATGAAGTGGCTTTCATAGTGTTCACCTTGCAGAAATAGAAACGCCCGACCTGCAACGAAGCGGGTCGGGCGTTATGGGGTGGGATTGGTAAAAGGTCAGGCGACCAATTGCAg                                                                                       >  6:179342/1‑147 (MQ=255)
                                                         agCGGGACGGATTTTGCTGGTGCTGCGATTCAATGAAGTGGCTTTCATAGTGTTCACCTTGCAGAAATAGAAACGCCCGACCTGCAACGAAGCGGGTCGGGCGTTATGGGGTGGGATTGGTAAAAGGTCAGGCGACCAATTGCa                                                                                        >  8:248692/1‑144 (MQ=255)
                                                         agCGGGACGGATTTTGCTGGTGCTGCGATTCAATGAAGTGGCTTTCATAGTGTTCACCTTGCAGAAATAGAAACGCCCGACCTGCAACGAAGCGGGTCGGGCGTTATGGGGTGGGATTGGTAAAAGGTCAGGCGACCAATTGCa                                                                                        <  7:248692/144‑1 (MQ=255)
                                                                     tttGCTGGTGCTGCGATTCAATGAAGTGGCTTTCATAGTGTTCACCTTGCAGAAATAGAAACGCCCGACCTGCAACGAAGCGGGTCGGGCGTTATGGGGTGGGATTGGTAAAAGGTCAGGCGACCAATTGCAGGGCAGTATCCAAGgc                                                                        <  6:64987/148‑1 (MQ=255)
                                                                          tGGTGCTGCGATTCAATGAAGTGGCTTTCATAGTGTTCACCTTGCAGAAATAGAAACGCCCGACCTGCAACGAAGCGGGTCGGGCGTTATGGGGTGGGATTGGTAAAAGGTCAGGCGACCAATTGCAGGGCAGTATCCAAGGCGCGTTg                                                                  >  5:315102/1‑149 (MQ=255)
                                                                            gTGCTGCGATTCAATGAAGTGGCTTTCTCTGTGTTCACCTTTCAGAAATAGAAACGCCCGACCTGCATCGAAGCGGGTCGGGCGTTATGGGGTGGGATTGGTAAAAGGTCAGGCGACCAATTGCAGGGCAGTATCCAAGGCGCGTTGCt                                                                <  5:202923/149‑1 (MQ=255)
                                                                                tgcGATTCAATGAAGTGGCTTTCATAGTGTTCACCTTGCAGAAATAGAAACGCCCGACCTGCAACGAAGCGGGTCGGGCGTTATGGGGTGGGATTGGTAAAAGGTCAGGCGACCAATTGCAGGGCAGTATCCAAGGCGCGTTGCTTGAt                                                            >  1:222095/1‑149 (MQ=255)
                                                                                tgcGATTCAATGAAGTGGCTTTCATAGTGTTCACCTTGCAGAAATAGAAACGCCCGACCTGCAACGAAGCGGGTCGGGCGTTATGGGGTGGGATTGGTAAAAGGTCAGGCGACCAATTGCAGGGCAGTATCCAAGGCGCGTTGCTTGAt                                                            >  5:271341/1‑149 (MQ=255)
                                                                                  cGATTCAATGAAGTGGCTTTCATAGTGTTCACCTTGCAGAAATAGAAACGCCCGACCTGCAACGAAGCGGGTCGGGCGTTATGGGGTGGGATTGGTAAAAGGTCAGGCGACCAATTGCAGGGCAGTATCCAAGGCGCGTTGCTTGAttt                                                          >  7:193468/1‑149 (MQ=255)
                                                                                  cGATTCAATGAAGTGGCTTTCATAGTCTTCACCTTGCAGAAATAGAAACGCCCGACCTGCAACGAACCGGGTCGGGCGTTATGGGGTGGGATTGGTAAAAGCTCAGGCGACCAATTGCAGGGCAGTATCCAAGGCGCGTTGCTTGAttt                                                          >  6:41735/1‑149 (MQ=255)
                                                                                   gATTCAATGAAGTGGCTTTCATAGTGTTCACCTTGCAGAAATAGAAACGCCCGACCTGCAACGAAGCGGGTCGGGCGTTATGGGGTGGGATTGGTAAAAGGTCAGGCGACCAATTGCAGGGCAGTATCCAAGGCGCGTTGCTTGATTTg                                                         >  3:170698/1‑149 (MQ=255)
                                                                                    aTTCAATGAAGTGGCTTTCATAGTGTTCACCTTGCAGAAATAGAAACGCCCGACCTGCAACGAAGCGGGTCGGGCGTTATGGGGTGGGATTGGTAAAAGGTCAGGCGACCAATTGCAGGGCAGTATCCAAGGCGCGTTGCTTGATTTgg                                                        <  8:82741/149‑1 (MQ=255)
                                                                                    aTTCAATGAAGTGGCTTTCATAGTGTTCACCTTGCAGAAATAGAAACGCCCGACCTGCAACGAAGCGGGTCGGGCGTTATGGGGTGGGATTGGTAAAAGGTCAGGCGACCAATTGCAGGGCAGTATCCAAGGCGCGTTGCTTGATTTgg                                                        <  1:201740/149‑1 (MQ=255)
                                                                                        aaTGAAGTGGCTTTCATAGTGTTCACCTTGCAGAAATAGAAACGCCCGACCTGCAACGAAGCGGGTCGGGCGTTATGGGGTGGGATTGGTAAAAGGTCAGGCGACCAAtt                                                                                           <  7:162844/110‑1 (MQ=255)
                                                                                        aaTGAAGTGGCTTTCATAGTGTTCACCTTGCAGAAATAGAAACGCCCGACCTGCAACGAAGCGGGTCGGGCGTTATGGGGTGGGATTGGTAAAAGGTCAGGCGACCAAtt                                                                                           >  8:162844/1‑110 (MQ=255)
                                                                                        aaTGAAGTGGCTTTCATAGTGTTCACCTTGCAGAAATAGAAACGCCCGACCTGCAACGAAGCGGGTCGGGCGTTATGGGGTGGGATTGGTAAAAGGTCAGGCGACCAATTGCAGGGCAGTATCCAAGGCGCGTTGCTTGATTTGGGCAc                                                    >  2:223692/1‑149 (MQ=255)
                                                                                         aTGAAGTGGCTTTCATAGTGTTCACCTTGCAGAAATAGAAACGCCCGACCTGCAACGAAGCGGGTCGGGCGTTATGGGTTGGGATTGGTAAAAGGTCAGGCGACCAATTGCAGGGCAGTATCCAAGGCGCGTTGCTTGATTTGGGCAcc                                                   >  3:94811/1‑149 (MQ=255)
                                                                                           gAAGTGGCTTTCATAGTGTTCACCTTGCAGAAATAGAAACGCCCGACCTGCAACGAAGCGGGTCGGGCGTTATGGGGTGGGATTGGTAAAAGGTCAGGCGACCAATTGCAGGGCAGTATCCAAGGCGCGTTGCTTGATTTGGGCACCCt                                                 >  7:119056/1‑149 (MQ=255)
                                                                                              gTGGCTTTCATAGTGTTCACCTTGCAGAAATAGAAACGCCCGACCTGCAACGAAGCGGGTCGGGCGTTATGGGGTGGGATTGGTAAAAGGTCAGGCGACCAATTGCAGGGCAGTATCCAAGGCGCGTTGCTTGATTTGGGCACCCTGGc                                              <  2:101328/149‑1 (MQ=255)
                                                                                              gTGGCTTTCATAGTGTTCACCTTGCAGAAATAGAAACGCCCGACCTGCAACGAAGCGGGTCGGGCGTTATGGGGTGGGATTGGTAAAAGGTCAGGCGACCAATTGCAGGGCAGTATCCAAGGCGCGTTGCTTGATTTGGGCACCCTGGc                                              <  2:222095/149‑1 (MQ=255)
                                                                                                ggCTTTCATAGTGTTCACCTTGCAGAAATAGAAACGCCCGACCTGCAACGAAGCGGGTCGGGCGTTATGGGGTGGGATTGGTAAAAGGTCAGGCGACCAATTGCAGGGCAGTATCCAAGGCGCGTTGCTTGATTTGGGCACCCTGGcc                                             >  4:200822/1‑148 (MQ=255)
                                                                                                ggCTTTCATAGTGTTCACCTTGCAGAAATAGAAACGCCCGACCTGCAACGAAGCGGGTCGGGCGTTATGGGGTGGGATTGGTAAAAGGTCAGGCGACCAATTGCAGGGCAGTATCCAAGGCGCGTTGCTTGATTTGGGCACCCTGGcc                                             >  8:113786/1‑148 (MQ=255)
                                                                                                       aTAGTGTTCACCTTGCAGAAATAGAAACGCCCGACCTGCAACGAAGCGGGTCGGGCGTTATGGGGTGGGATTGGTAAAAGGTCAGGCGACCAATTGCA‑‑GCAGTATCCAAGGCGCGTTGCTTGATTTGGGCACCCTGGCCGAACCAGGc                                    <  5:179342/148‑1 (MQ=255)
                                                                                                       aTAGTGTTCACCTTGCAGAAATAGAAACGCCAGACCTGCAACGACGCGGTTCGGGCGTTATGGGGTGGGATTGGTAAAAGGTCAGGCGACCAATTGCAGGGCAGTATCCAAGGCGCGTTGCTTGATTTGGGCACCCTGGCCGAACCAgt                                     <  6:228007/149‑2 (MQ=255)
                                                                                                        tAGTGTTCACCTTGCAGAAATAGAAACGCCCGACCTGCAACGAAGCGGGTCGGGCGTTATGGGGTGGGATTGGTAAAAGGTCAGGCGACCAATTGCAGGGCAGTATCCAAGGCGCGTTGCTTGATTTGGGCACCCTGGCCGAACCAGGc                                    <  6:315102/149‑1 (MQ=255)
                                                                                                           tgtTCACCTTGCAGAAATAGAAACGCCCGACCTGCAACGAAGCGGGTCGGGCGTTATGGGGTGGGATTGGTAAAAGGTCAGGCGACCAATTGCAGGGCAGTATCCAAGGCGCGTTGCTTGATTTGGGCACCCTGGCCGAACCAGGCGGa                                 <  8:119056/149‑1 (MQ=255)
                                                                                                               cACCTTGCAGAAATAGAAACGCCCGACCTGCAACGAAGCGGGTCGGGCGTTATGGGGTGGGATTGGTAAAAGGTCAGGCGACCAATTGCAGGGCAGTATCCAAGGCGCGTTGCTTGATTTGGGCACCCTGGCCGAACCAGGCGGAGTc                              >  3:46964/1‑148 (MQ=255)
                                                                                                               cACCTTGCAGAAATAGAAACGCCCGACCTGCAACGAAGCGGGTCGGGCGTTATGGGGTGGGATTGGTAAAAGGTCAGGCGACCAATTGCAGGGCAGTATCCAAGGCGCGTTGCTTGATTTGGGCACCCTGGCCGAACCAGGCGGAGTc                              <  4:170698/148‑1 (MQ=255)
                                                                                                                     gCAGAAATAGAAACGCCCGACCTGCAACGAAGCGGGTCGGGCGTTATGGGGTGGGATTGGTAAAAGGTCAGGCGACCAATTGCAGGGCAGTATCCAAGGCGCGTTGCTTGATTTGGGCACCCTGGCCGAACCAGGCGGAGTCGAGGc                         <  8:193468/147‑1 (MQ=255)
                                                                                                                      cAGAAATAGAAACGCCCGACCTGTAACGAAGCGGGTCGGGCGTTATGGGGTGGGATTGGTAAAAGGTCAGGCGACCAATTGCAGGGCAGTATCCAAGGCGCGTTGCTTGGTTTGGGCACCCTGGCCGAACCAgg                                     <  4:105488/134‑1 (MQ=255)
                                                                                                                               aaaCGCCCGACCTGCAACGAAGCGGGTCGGGCGTTATGGGGTGGGATTGGTAAAAGGTCAGGCGACCAATTGCAGGGCAGTATCCAAGGCGCGTTGCTTGATTTGGGCACCCTGGCCGAAc                                         <  5:316043/121‑1 (MQ=255)
                                                                                                                               aaaCGCCCGACCTGCAACGAAGCGGGTCGGGCGTTATGGGGTGGGATTGGTAAAAGGTCAGGCGACCAATTGCAGGGCAGTATCCAAGGCGCGTTGCTTGATTTGGGCACCCTGGCCGAAc                                         >  6:316043/1‑121 (MQ=255)
                                                                                                                                aaCGCCCGACCTGCAACGAAGCGGGTCGGGCGTTATGGGGTGGGATTGGTAAAAGGTCAGGCGACCAATTGCAGGGCAGTATCCAAGGCGCGTTGCTTGATTTGGGCACCCTGGCCGAACCAGGCGGAGTCGAGGCGGTATTCGGtgct            <  1:223692/149‑1 (MQ=255)
                                                                                                                                  cGCCCGACCTGCAACGAAGCGGGTCGGGCGTTATGGGGTGGGATTGGTAAAAGGTCAGGCGACCAATTGCAGGGCAGTATCCAAGGCGCGTTGCTTGATTTGGGCACCCTGGCCGAACCAGGCGGAGTCGAGGCGGTATTCGGtgctgc          <  6:271341/149‑1 (MQ=255)
                                                                                                                                   gCCCGACCCGCTTCGTTGCAGGTCGGGCGTTATGGGGTGGGATTGGTAAAAGGTCAGGCGACCAATTGCAGGGCAGTATCCAAGGCGCGTTGCTTGATTTGGGCACCCTGGCCGAACCAGGCGGAGTCGAGGCGGTATTCGGTGCTGCg         >  7:205635/1‑149 (MQ=255)
                                                                                                                                    cccGACCCGCTTCGTTGCAGGTCGGGCGTTATGGGGTGGGATTGGTAAAAGGTCAGGCGACCAATTGCAGGGCAGTATCCAAGGCGCGTTGCTTGATTTGGGCACCCTGGCCGAACCAGGCGGAGTCGAGGCGGTATTCGGTGCTGCGt        >  4:86061/1‑149 (MQ=255)
                                                                                                                                          cTGCAACGAAGCGGGTCGGGCGTTATGGGGTGGGATTGGTAAAAGGTCAGGCGACCAATTGCAGGGCAGTATCCAAGGCGCGTTGCTTGATTTGGGCACCCTGGCCGAACCAGGCGGAGTCGAGGCGGTATTCGGTGCTGCGTGCccgc  <  7:113786/149‑1 (MQ=255)
                                                                                                                                              |                                                                                                                                                
CTTGCGGATCTGCAGCCAGTCCTCGCGGGGTAGCTCCTTGGTGCTAACCAGGCGCAGAGCGGGACGGATTTTGCTGGTGCTGCGATTCAATGAAGTGGCTTTCATAGTGTTCACCTTGCAGAAATAGAAACGCCCGACCTGCAACGAAGCGGGTCGGGCGTTATGGGGTGGGATTGGTAAAAGGTCAGGCGACCAATTGCAGGGCAGTATCCAAGGCGCGTTGCTTGATTTGGGCACCCTGGCCGAACCAGGCGGAGTCGAGGCGGTATTCGGTGCTGCGTGCCCGC  >  NC_002947/2844536‑2844822

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑