Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,850,718 G→A 7.8% E170E (GAG→GAA)  PP_2503 → 3‑oxoacyl‑ACP reductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,850,7180GA 7.8% 147.5 / 2.2 51E170E (GAG→GAA) PP_25033‑oxoacyl‑ACP reductase
Reads supporting (aligned to +/- strand):  ref base G (27/20);  new base A (2/2);  total (29/22)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.69e-01

GCCGCCGCGCACATGATCGAGCGGCGCCAGGGTCACATCATCAACATCACTGCTGCCGTGGCGCTGCAACCCAACATCGCCGTGCCCGCCGCCCTGCCGGTGCTGATCAAGGGGGGCCTCAACCAGGTCACCCGCGCCCTGGCGCTGGAGCTGTCGTCGTACAACATCCAGGTTAACGCGGTGGCTCCCGGCATCATCGAGACGCCGATGCACGATCCGGCCAGCTTCGACTTCCTCAACGGTCTGCAACCGGCCGGACACATCGGCCGCGTCAGCGACATTGCCGACGCCGTG  >  NC_002947/2850569‑2850862
                                                                                                                                                     |                                                                                                                                                
gccgccgcGCACATGATCGAGCGGCGCCAGGGTCACATCATCAACATCACTGCTGCCGTGGCGCTGCAACCCAACATCGCCGTGCCCGCCGCCCTGCCGGTGGTGATCAA‑GGGGGCCTCAACCAGGTCACCCGCGCCCTGGCGCTGGAg                                                                                                                                                  <  4:161117/149‑1 (MQ=255)
 ccgccgcGCACATGATCGAGCGGCGCCAGGGTCACATCATCAACATCACTGCTGCCGTGGCGCTGCAACCCAACATCGCCGTGCCCGCCGCCCTGCCGGTGCTGATCAAGGGGGGCCTCAACCAGGTCACCCGCGCCCTGGCGCTGGAg                                                                                                                                                  <  1:230039/149‑1 (MQ=255)
       cgcACATGATCGAGCGGCGCCAGGGTCACATCATCAACATCACTGCTGCCGTGGCGCTGCAACCCAACATCGCCGTGCCCGCCGCCCTGCCGGTGCTGATCAAGGGGGGCCTCAACCAGGTCACCCGCGCCCTGGCGCTGGAGCTgtcg                                                                                                                                            <  7:116082/149‑1 (MQ=255)
           caTGATCGAGCGGCGCCAGGGTCACATCATCAACATCACTGCTGCCGTGGCGCTGCAACCCAACATCGCCGTGCCCGCCGCCCTGCCGGTGCTGATCAAGGGGGGCCTCAACCAGGTCACCCGCGCCCTGGCGCTGGAGCTGtcgtcg                                                                                                                                         >  7:151501/1‑148 (MQ=255)
                tCGAGCGGCGCCAGGGTCACATCATCAACATCACTGCTGCCGTGGCGCTGCAACCCAACATCGCCGTGCCCGCCGCCCTGCCGGTGCTGATCAAGGGGGGCCTCAACCAGGTCACCCGCGCCCTGGCGCTGGAGCTGTCGTCGTacaa                                                                                                                                    >  7:116023/1‑148 (MQ=255)
                                tCACATCATCAACATCACTGCTGCCGTGGCGCTGCAACCCAACATCGCCGTGCCCGCCGCCCTGCCGGTGCTGATCAAGGGGGGCCTCAACCAGGTCACCCGCGCCCTGGCGCTGGAGCTGTCGTCGTACCACATCCAGGTTACCGCgg                                                                                                                   >  2:326228/1‑149 (MQ=255)
                                  acaTCATCAACATCACTGCTGCCGTGGCGCTGCAACCCAACATCGCCGTGCCCGCCGCCCTGCCGGTGCTGATCAAGGGGGGCCTCAACCAGGTCTCCCGCGCCCTGGCGCTGGAGCTGTCGTCGTACAACATCCAGGTTAACGCGGt                                                                                                                  <  6:156389/148‑1 (MQ=255)
                                  acaTCATCAACATCACTGCTGCCGTGGCGCTGCAACCCAACATCGCCGTGCCCGCCGCCCTGCCGGTGCTGATCAAGGGGGGCCTCAACCAGGTCACCCGCGCCCTGGCGCTGGAGCTGTCGTCGTACAACATCCAGGTTAACGCGGTg                                                                                                                 <  4:35451/149‑1 (MQ=255)
                                  acaTCATCAACATCACTGCTGCCGTGGCGCTGCAACCCAACATCGCCGTGCCCGCCGCCCTGCCGGTGCTGATCAAGGGGGGCCTCAACCAGGTCACCCGCGCCCTGGCGCTGGAGCTGTCGTCGTACAACATCCAGGTTAACGCGGTg                                                                                                                 >  6:319281/1‑149 (MQ=255)
                                        tcaACATCACTGCTGCCGTGGCGCTGCAACCCAACATCGCCGTGCCCGCCGCCCTGCCGGTGCTGATCAAGGGGGGCCTCAACCAGGTCACCCGCGCCCTGGCGCTGGAACTGTCGTCGTACAACATCCAGGTTAACGCGGTGGCTccc                                                                                                           >  8:103242/1‑149 (MQ=255)
                                        tcaACATCACTGCTGCCGTGGCGCTGCAACCCAACATCGCCGTGCCCGCCGCCCTGCCGGTGCTGATCAAGGGGGGCCTCAACCAGGTCACCCGCGCCCTGGCGCTGGAACTGTCGTCGTACAACATCCAGGTTAACGCGGTGGCTccc                                                                                                           >  4:37222/1‑149 (MQ=255)
                                           aCATCACTGCTGCCGTGGCGCTGCAACCCAACATCGCCGTGCCCGCCGCCCTGCCGGTGCTGATCAAGGGGGGCCTCAACCAGGTCACCCGCGCCCTGGCGCTGGAGCTGTCGTCGTACAACATCCAGGTTAAc                                                                                                                       <  8:32807/134‑1 (MQ=255)
                                           aCATCACTGCTGCCGTGGCGCTGCAACCCAACATCGCCGTGCCCGCCGCCCTGCCGGTGCTGATCAAGGGGGGCCTCAACCAGGTCACCCGCGCCCTGGCGCTGGAGCTGTCGTCGTACAACATCCAGGTTAAc                                                                                                                       >  7:32807/1‑134 (MQ=255)
                                           aCATCACTGCTGCCGTGGCGCTGCAACCCAACATCGCCGTGCCCGCCGCCCTGCCGGTGCTGATCAAGGGGGGCCTCAACCAGGTCACCCGCGCCCTGGCGCTGGAGCTGTCGTCGTACAACATCCAGGTTAACGCGGTGGCTCCCgg                                                                                                         >  5:70222/1‑148 (MQ=255)
                                               cACTGCTGCCGTGGCGCTGCAACCCAACATCGCCGTGCCCGCCGCCCTGCCGGTGCTGATCAAGGGGGGCCTCAACCAGGTCACCCGCGCCCTGGCGCTGGAGCTGTCGTCGTACAACATCCAGGTTAACGCGGTGGCTCCCGGcatca                                                                                                    >  5:137447/1‑149 (MQ=255)
                                                      gcCGTGGCGCTGCAACCCAACATCGCCGTGCCCGCCGCCCTGCCGGTGCTGATCAAGGGGGGCCTCAACCAGGTCACCCGCGCCCTGGCGCTGGAGCTGTCGTCGTACAACATCCAGGTTAACGCGGTGGCTCCCGGCATCATCGAGAc                                                                                             >  5:138875/1‑149 (MQ=255)
                                                              gcTGCAACCCAACATCGCCGTGCCCGCCGCCCTGCCGGTGCTGATCAAGGGGGGCCTCAACCAGGTCACCCGCGCCCTGGCGCTGGAGCTGTCGTCGTACAACATCCAGGTTAACGCGGTGGCTCCCGGCATCATCGAGACGCCGATg                                                                                      >  4:149593/1‑148 (MQ=255)
                                                                    aCCCAACATCGCCGTGCCCGCCGCCCTGCCGGTGCTGATCAAGGGGGGCCTCAACCAGGTCACCCGCGCCCTGGCGCTGGAGCTGTCGTCGTACAACATCCAGGTTAACGCGGTGGCTCCCGGCATCATCGAGACGCCGATGCACGATc                                                                               >  6:42251/1‑149 (MQ=255)
                                                                    aCCCAACATCGCCGTGCCCGCCGCCCTGCCGGTGCTGATCAAGGGGGGCCTCAACCAGGTCACCCGCGCCCTGGCGCTGGAACTGTCGTCGTACAACATCCAGGTTAACGCGGTGGCTCCCGGCATCATCGAGACGCCGATGCACGATc                                                                               <  7:103242/149‑1 (MQ=255)
                                                                    aCCCAACATCGCCGTGCCCGCCGCCCTGCCGGTGCTGATCAAGGGGGGCCTCAACCAGGTCACCCGCGCCCTGGCGCTGGAACTGTCGTCGTACAACATCCAGGTTAACGCGGTGGCTCCCGGCATCATCGAGACGCCGATGCACGATc                                                                               <  3:37222/149‑1 (MQ=255)
                                                                     cccAACATCGCCGTGCCCGCCGCCCTGCCGGTGCTGATCAAGGGGGGCCTCAACCAGGTCACCCGCGCCCTGGCGCTGGAGCTGTCGTCGTACAACATCCAGGTTAACGCGGTGGCTCCCGGCATCATCGAGACGCCGATGCACGATcc                                                                              >  4:168052/1‑149 (MQ=255)
                                                                       cAACATCGCCGTGCCCGCCGCCCTGCCGGTGCTGATCAAGGGGGGCCTCAACCAGGTCACCCGCGCCCTGGCGCTGGAGCTGTCGTCGTACAACATCCAGGTTAACGCGGTGGCTCCCGGCATCATCGAGACGCCGATGCACGATCCgg                                                                            >  3:220244/1‑149 (MQ=255)
                                                                       cAACATCGCCGTGCCCGCCGCCCTGCCGGTGCTGATCAAGGGGGGCCTCAACCAGGTCACCCGCGCCCTGGCGCTGGAGCTGTCGTCGTACAACATCCAGGTTAACGCGGTGGCTCCCGGCATCATCGAGACGCCGATGCACGATCCgg                                                                            <  3:168052/149‑1 (MQ=255)
                                                                        aaCATCGCCGTGCCCGCCGCCCTGCCGGTGCTGATCAAGGGGGGCCTCAACCAGGTCACCCGCGCCCTGGCGCTGGAGCTGTCGTCGTACAACATCCAGGTTAACGCGGTGGCTCCCGGCATCATCGAGACGCCGATGCACGATCCGGc                                                                           >  3:144362/1‑149 (MQ=255)
                                                                           aTCGCCGTGCCCGCCGCCCTGCCGGTGCTGATCAAGGGGGGCCTCAACCAGGTCACCCGCGCCCTGGCGCTGGAGCTGTCGTCGTACAACATCCAGGTTAACGCGGTGGCTCCCGGCATCATCGAGACGCCGATGCACGATCCGGCCAg                                                                        <  6:137447/149‑1 (MQ=255)
                                                                                 gTGCCCGCCGCCCTGCCGGTGCTGATCAAGGGGGGCCTCAACCAGGTCACCCGCGCCCTGGCGCTGGAGCTGTCGTCGTACAACATCCAGGTTAACGCGGTGGCTCCCGGCATCATCGAGACGCCGATGCACGATCCGGCCAGCTTCGa                                                                  <  6:138875/149‑1 (MQ=255)
                                                                                        ccgccCTGCCGGTGCTGATCAAGGGGGGGCTCAACCAGGTCACCCGCGCCCTGGCGCTGGAGCTGTCGTCGTACAACATCCAGGTTAACGCGGTGGCTCCCGGCATCATCGAGACGCCGATGCACGATCCGGCCAGCTTCGACTTCCt                                                            >  1:190814/1‑148 (MQ=255)
                                                                                             cTGCCGGTGCTGATCAAGGGGGGCCTCAACCAGGTCACCCGCGCCCTGGCGCTGGAGCTGTCGTCGTACAACATCCAGGTTAACGCGGTGGTTCCCGGCATCATCGAGACGCCGATGCACGATCCGGCCAGCTTCGACTTCCTCAACgg                                                      >  4:54519/1‑149 (MQ=255)
                                                                                                 cGGTGCTGATCAAGGGGGGCCTCAACCAGGTCACCCGCGCCCTGGCGCTGGAGCTGTCGTCGTACAACATCCAGGTTAACGCGGTGGCTCCCGGCATCATCGAGACGCCGATGCACGATCCGGCCAGCTTCGACTTCCt                                                            >  8:256522/1‑139 (MQ=255)
                                                                                                 cGGTGCTGATCAAGGGGGGCCTCAACCAGGTCACCCGCGCCCTGGCGCTGGAGCTGTCGTCGTACAACATCCAGGTTAACGCGGTGGCTCCCGGCATCATCGAGACGCCGATGCACGATCCGGCCAGCTTCGACTTCCt                                                            <  7:256522/139‑1 (MQ=255)
                                                                                                   gTGCTGATCAAGGGGGGCCTCAACCAGGTCACCCGCGCCCTGGCGCTGGAGCTGTCGTCGTACAACATCCAGGTTAACGCGGTGGCTCCCGGCATCATCGAGACGCCGATGCACGATCCGGCCAGCTTCGACTTCCTCAACGGTCTGc                                                 <  1:103773/148‑1 (MQ=255)
                                                                                                   gTGCTGATCAAGGGGGGCCTCAACCAGGTCACCCGCGCCCTGGCGCTGGAGCTGTCGTCGTACAACATCCAGGTTAACGCGGTGGCTCCCGGCATCATCGAGACGCCGATGCACGATCCGGCCAGCTTCGACTTCCTCAACGGTCTGc                                                 >  2:103773/1‑148 (MQ=255)
                                                                                                       tGATCAAGGGGGGCCTCAACCAGGTCACCCGCGCCCTGGCGCTGGAGCTGTCGTCGTACAACATCCAGGTTAACGCGGTGGCTCCCGGCATCATCGAGACGCCGATGCACGATCCGGCCAGCTTCGACTTCCTCAACGGTCTGCAAccg                                            >  2:91628/1‑149 (MQ=255)
                                                                                                         aTCAAGGGGGGCCTCAACCAGGTCACCCGCGCCCTGGCGCTGTAGCTGTCGTCGTACAACATCCAGGTTAACGCGGTGGCTCCCGGCATCATCGAGACGCCGATGCACGATCCGGCCAGCTTCGACTTCCTCAACGGTCt                                                   >  5:132586/1‑140 (MQ=255)
                                                                                                         aTCAAGGGGGGCCTCAACCAGGTCACCCGCGCCCTGGCGCTGGAGCTGTCGTCGTACAACATCCAGGTTAACGCGGTGGCTCCCGGAATCATCGAGACGCCGATGCACGATCCGGCCAGCTTCGACTTCCTCAACGGTCt                                                   <  6:132586/140‑1 (MQ=255)
                                                                                                            aaGGGGGGCCTCAACCAGGTCACCCGCGCCCTGGCGCTGGAGCTGTCGTCGTACAACATCCAGGTTAACGCGGTGGCTCCCGGCATCATCGAGACGCCGATGCACGATCCGGCCAGCTTCGACTTCCTCAACGGTCTGCAAccggccg                                        >  7:31775/1‑148 (MQ=255)
                                                                                                             aGGGGGGCCTCAACCAGGTCACCCGCGCCCTGGCGCTGGAGCTGTCGTCGTACAACATCCAGGTTAACGCGGTGGCTCCCGGCATCATCGAGACGCCGATGCACGATCCGGCCAGCTTCGACTTCCTCAACGGTCTGCAACCGGCCGGa                                      <  7:67220/149‑1 (MQ=255)
                                                                                                              ggggggCCTCAACCAGGTCACCCGCGCCCTGGCGCTGGAGCTGTCGTCGTACAACATCCAGGTTAACGCGGTGGCTCCCGGCATCATCGAGACGCCGATGCACGATCCGGCCAGCTTCGACTTCCTCAACGGTCTGCAACCGGCCGGac                                     <  2:190814/149‑1 (MQ=255)
                                                                                                                    ccTCAACCAGGTCACCCGCGCCCTGGCGCTGGAGCTGTCGTCGTACAACATCCAGGTTAACGCGGTGGCTCCCGGCATCATCGAGACGCCGATGCACGATCCGGCCAGCTTCGACTTCCTCAACGGTCTGCAACCGGCCGGACACATCg                               >  8:91830/1‑149 (MQ=255)
                                                                                                                      tCAACCAGGTCACCCGCGCCCTGGCGCTGGAGCTGTCGTCGTACAACATCCAGGTTAACGCGGTGGCTCCCGGCATCATCGAGACGCCGATGCACGATCCGGCCAGCTTCGACTTCCTCAACGGTCt                                                   <  4:114562/127‑1 (MQ=255)
                                                                                                                      tCAACCAGGTCACCCGCGCCCTGGCGCTGGAGCTGTCGTCGTACAACATCCAGGTTAACGCGGTGGCTCCCGGCATCATCGAGACGCCGATGCACGATCCGGCCAGCTTCGACTTCCTCAACGGTCt                                                   >  3:114562/1‑127 (MQ=255)
                                                                                                                      tCAACCAGGTCACCCGCGCCCTGGCGCTGGAGCTGTCGTCGTACAACATCCAGGTTAACGCGGTGGCTCCCGGCATCATCGAGACGCCGATGCACGATCCGGCCAGCTTCGACTTCCTCAACGGTCTGCAACCGGCCGGACACATCGGc                             >  5:323891/1‑149 (MQ=255)
                                                                                                                      tCAACCAGGTCACCCGCGCCCTGGCGCTGGAGCTGTCGTCGTACAACATCCAGGTTAACGCGGTGGCTCCCGGCATCATCGAGACGCCGATGCACGATCCGGCCAGCTTCGACTTCCTCAACGGTCTGCAACCGGCCGGACACATCGGc                             >  5:116847/1‑149 (MQ=255)
                                                                                                                           cAGGTCACCCGCGCCCTGGCGCTGGAGCTGTCGTCGTACAACATCCAGGTTAACGCGGTGGCTCCCGGCATCATCGAGACGCCGATGCACGATCCGGCCAGCTTCGACTTCCTCAACGGTCTGCAACCGGCCGGACACATCGGCCGCGt                        <  8:151501/149‑1 (MQ=255)
                                                                                                                               tCACCCGCGCCCTGGCGCTGGAGCTGTCGTCGTACAACATCCAGGTTAACGCGGTGGCTCCCGGCATCATCGAGACGCCGATGCACGATCCGGCCAGCTTCGACTTCCTCAACGGTCTGCAACCGGCCGGACACATCGGCCGCGTCAGc                    >  3:155020/1‑149 (MQ=255)
                                                                                                                                 aCCCGCGCCCTGGCGCTGGAGCTGTCGTCGTACAACATCCAGGTTAACGCGGTGGCTCCCGGCATCATCGAGACGCCGATGCACGATCCGGCCAGCTTCGACTTCCTCAACGGTCTGCAACCGGCCGGACACATCGGCCGCGTCAGCGa                  >  6:131622/1‑149 (MQ=255)
                                                                                                                                   ccGCGCCCTGGCGCTGGAGCTGTCGTCGTACAACATCCAGGTTAACGCGGTGGCTCCCGGCATCATCGAGACGCCGATGCACGATCCGGCCAGCTTCGACTTCCTCAACGGTCTGCAACCGGCCGGGCACATCGGCCGCGTCAGCGACa                >  5:113702/1‑149 (MQ=255)
                                                                                                                                        cccTGGCGCTGGAGCTGTCGTCGTACAACATCCAGGTTAACGCGGTGGCTCCCGGCATCATCGAGACGCCGATGCACGATCCGGCCAGCTTCGACTTCCTCAACGGTCTGCAACCGGCCGGACACATCGGCCGCGTCAGCGACATTGcc           <  6:70222/149‑1 (MQ=255)
                                                                                                                                          cTGGCGCTGGAGCTGTCGTCGTACAACATCCAGGTTAACGCGGTGGCTCCCGGCATCATCGAGACGCCGATGCACGATCCGGCCAGCTTCGACTTCCTCAACGGTCTGCAACCGGCCGGACACATCGGCCGCGTCAGCGACATTGCCGa         <  4:155020/149‑1 (MQ=255)
                                                                                                                                             gcgcTGGAGCTGTCGTCGTACAACATCCAGGTTAACGCGGTGGCTCCCGGCATCATCGAGACGCCGATGCACGATCCGGCCAGCTTCGACTTCCTCAACGGTCTGCAACCGGCCGGTCACATCGGCCGCGTCAGCGACATTGCCGAc        <  6:113702/147‑1 (MQ=255)
                                                                                                                                                cTGGAGCTGTCGTCGTACAACATCCAGGTTCACGCGGGGGCTCCCGGCATCATCGAGACGCCGATGCACGATCCGGCCAGCTTCGACTTCCTCAACGGTCTGCAACCGGCCGGACACATCGGCCGCGTCAGCGACATTGCCGACGCCGt   <  4:255651/149‑1 (MQ=255)
                                                                                                                                                cTGGAGCTGTCGTCGTACAACATCCAGGTTAACGCGGTGGTTCCCGGCATCATCGAGACGCCGATGCACGATCCGGCCAGCTTCGACTTCCTCAACGGTCTGCAACCGGCCGGACACATCGGCCGCGTCAGCGACATTGCCGACGCCGt   <  3:54519/149‑1 (MQ=255)
                                                                                                                                                   gAGCTGTCGTCGTACAACATCCAGGTTAACGCGGTGGCTCCCGGCATCATCGAGACGCCGATGCACGATCCGGCCAGCTTCGACTTCCTCAACGGTCTGCAACCGGCCGGACACATCGGCCGCGTCAGCGACATTGCCGACGCCGTg  <  7:91830/147‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                
GCCGCCGCGCACATGATCGAGCGGCGCCAGGGTCACATCATCAACATCACTGCTGCCGTGGCGCTGCAACCCAACATCGCCGTGCCCGCCGCCCTGCCGGTGCTGATCAAGGGGGGCCTCAACCAGGTCACCCGCGCCCTGGCGCTGGAGCTGTCGTCGTACAACATCCAGGTTAACGCGGTGGCTCCCGGCATCATCGAGACGCCGATGCACGATCCGGCCAGCTTCGACTTCCTCAACGGTCTGCAACCGGCCGGACACATCGGCCGCGTCAGCGACATTGCCGACGCCGTG  >  NC_002947/2850569‑2850862

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑