Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,863,245 G→C 7.8% R102P (CGG→CCG)  gllR → HTH‑type transcriptional regulator GalR

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,863,2450GC 7.8% 159.9 / 4.6 51R102P (CGG→CCG) gllRHTH‑type transcriptional regulator GalR
Reads supporting (aligned to +/- strand):  ref base G (26/21);  new base C (2/2);  total (28/23)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.49e-01

CGAGCGCCATGCCAATGGCATGCGCCTGACCGACTATGGCGAGTGCCTGCTGCCGCGTGCACAGCGCGTGCTGGCGGAACTGGACGGCGTGCCGAGTTTGCTGGGCACCGCGCAGGGCGAGCCCCTCTACCTGTTTCAGGCGCGGCGCCTGCAGGTGTTCGTGAAGCTCTGTGAAACACGGCACATGCAAACCGTGGCGCGGCACTTCGGCCTTAGCCAGCCGGCGGTCAGTGCTGCTTTGAAAGTGCTCGAAGGCGGGTGCGGACAGCCCTTGTTCGTGCGCACGTCGCGT  >  NC_002947/2863102‑2863393
                                                                                                                                               |                                                                                                                                                    
cGAGCGCCATGCCAATGGCATGCGCCTGACCGACTATGGCGAGTGCCTGCTGCCGCGTGCACAGCGCGTGCTGGCGGAACTGGACGGCGTGCCGAGTTTGCTGGGCACCGCGCAGGGCGAGCCCCTCTACCTGTTTCAggcgcggcgc                                                                                                                                                  <  5:323003/148‑1 (MQ=255)
  cgcagcATGCCAATGGCATGCGCCTGACCGACTATGGCGAGTGCCTGCTGCCGCGTGCACAGCGCGTGCTGGCGGAACTGGACGGCGTGCCGAGTTTGCTGGGCACCGCGCAGGGCGAGCCCCTCTACCTGTTTCAGGCGCGGCGCCTg                                                                                                                                               <  6:211190/144‑1 (MQ=255)
            cAATGGCATGCGCCTGACCGACTATGGCGAGTGCCTGCTGCCGCGTGCACAGCGCGTGCTGGCGGAACTGGACGGCGTGCCGAGTTTGCTGGGCACCGCGCAGGGCGAGCCCCTCTACCTGTTTCAGGCGCGGCGCCTGCAGGTGTTCg                                                                                                                                     >  5:282774/1‑149 (MQ=255)
                  cATGCGCCTGACCGACTATGGCGAGTGCCTGCTGCCGCGTGCACAGCGCGTGCTGGCGGAACTGGACGGCGTGCCGAGTTTGCTGGGCACCGCGCAGGGCGAGCCCCTCTACCTGTTTCAGGCGCGGCGCCTGCAGGTGTTCGTGAAGc                                                                                                                               >  6:264471/1‑149 (MQ=255)
                   aTGCGCCTGACCGACTATGGCGAGTGCCTGCTGCCGCGTGCACAGCGCGTGCTGGCGGAACTGGACGGCGTGCCGAGTTTGCTGGGCACCGCGCAGGGCGAGCCCCTCTACCTGTTTCAGGCGCGGCGCCTGCAGGTGTTCGTGAAGct                                                                                                                              <  6:213370/149‑1 (MQ=255)
                             ccGACTATGGCGAGTGCCTGCTGCCGCGTGCACAGCGCGTGCTGGCGGAACTGGACGGCGTGCCGAGTTTGCTGGGCACCGCGCAGGGCGAGCCCCTCTACCTGTTTCAGGCGCGGCGCCTGCAGGTGTTCGTGAAGCTCTGTGAAaca                                                                                                                    <  5:264471/149‑1 (MQ=255)
                              cGACTATGGCGAGTGCCTGCTGCCGCGTGCACAGCGCGTGCTGGCGGAACTGGACGGCGTGCCGAGTTTGCTGGGCACCGCGCAGGGCGAGCCCCTCTACCTGTTTCAGGCGCGGCGCCTGCAGGTGTTCGTGAAGCTCTGTGAAacac                                                                                                                   >  1:157224/1‑149 (MQ=255)
                               gACTATGGCGAGTGCCTGCTGCCGCGTGCACAGCGCGTGCTGGCGGAACTGGACGGCGTGCCGAGTTTGCTGGGCACCGCGCAGGGCGAGCCCCTCTACCTGTTTCAGGCGCGGCGCCTGCAGGTGTTCGTGAAGCTCTGTGAAACACg                                                                                                                  >  2:157239/1‑149 (MQ=255)
                                      gCGAGTGCCTGCTGCCGCGTGCACAGCGCGTGCTGGCGGAACTGGACGGCGTGCCGAGTTTGCTGGGCACCGCGCAGGGCGAGCCCCTCTACCTGCTTCAGGCGCGGCGCCTGCAGGTGTTCGTGAAGCTCTGTGAAACACGGCACAt                                                                                                            <  3:125099/148‑1 (MQ=255)
                                      gCGAGTGCCTGCTGCCGCGTGCACAGCGCGTGCTGGCGGAACTGGACGGCGTGCCGAGTTTGCTGGGCACCGCGCAGGGCGAGCCCCTCTACCTGCTTCAGGCGCGGCGCCTGCAGGTGTTCGTGAAGCTCTGTGAAACACGGCACAt                                                                                                            >  4:125099/1‑148 (MQ=255)
                                              ctgctgCCGCGTGCACAGCGCGTGCTGGCGGAACTGGACGGCGTGCCGAGTTTGCTGGGCACCGCGCAGGGCGAGCCCCTCTACCTGTTTCAGGCGCCGCGCCTgaaac                                                                                                                                           <  3:156194/109‑5 (MQ=255)
                                              ctgctgCCGCGTGCACAGCGCGTGCTGGCGGAACTGGACGGCGTGCCGAGTTTGCTGGGCACCGCGCAGGGCGAGCCCCTCTACCTGTTTCAGGCGCCGCGCCTgaaac                                                                                                                                           >  4:156194/1‑105 (MQ=255)
                                                          gCACAGCGCGTGCTGGCGGAACTGGACGGCGTGCCGAGTTTGCTGGGCACCGCGCAGGGCGAGCCCCTCTACCTGTTTCAGGCGCGGCGCCTGCAGGTGTTCGTGAAGCTCTGTGAAACACGGCACATGCAAACCGTGGCGCGGCACtt                                                                                       >  1:204784/1‑149 (MQ=255)
                                                           cacaGCGCGTGCTGGCGGAACTGGACGGCGTGCCGAGTTTGCTGGGCACCGCGCAGGGCGAGCCCCTCTACCTGTTTCAGGCGCGGCGCCTGCAGGTGTTCGTGAAGCTCTGTGAAACACGGCACATGCAAACCGTGGCGCGGCACTTc                                                                                      >  6:36766/1‑149 (MQ=255)
                                                            acaGCGCGTGCTGGCGGAACTGGACGGCGTGCCGAGTTTGCTGGGCACCGCGCAGGGCGAGCCCCTCTACCTGTTTCAGGCGCGGCGCCTGCAGGTGTTCGTGAAGCTCTGTGAAACACGGCACATGCAAACCGTGGCGCGGCACTTCg                                                                                     <  5:293315/149‑1 (MQ=255)
                                                            acaGCGCGTGCTGGCGGAACTGGACGGCGTGCCGAGTTTGCTGGGCACCGCGCAGGGCGAGCCCCTCTACCTGTTTCAGGCGCGGCGCCTGCAGGTGTTCGTGAAGCTCTGTGAAACACGGCACATGCAAACCGTGGCGCGGCACTTCg                                                                                     >  4:323528/1‑149 (MQ=255)
                                                            acaGCGCGTGCTGGCGGAACTGGACGGCGTGCCGAGTTTGCTGGGCACCGCGCAGGGCGAGCCCCTCTACCTGTTTCAGGCGCGGCGCCTGCAGGTGTTCGTGAAGCTCTGTGAAACACGGCACATGCAAACCGTGGCGCGGCACTTCg                                                                                     >  4:62414/1‑149 (MQ=255)
                                                              aGCGCGTGCTGGCGTAAGTGGACGGCGTGCCGAGATTGCTGGGCACCGCGCAGGGCGAGCCCCTCTACCTGTTTCAGGCGCGGCGCCTGCAGGTGTTCGTGAAGCTCTGTGAAACACGGCACATGCAAACCGTGGCGCGGCACTTCGGc                                                                                   <  6:282774/149‑1 (MQ=255)
                                                               gcgcGTGCTGGCGGAACTGGACGGCGTGCCGAGTTTGCTGGGCACCGCGCAGGGCGAGCCCCTCTACCTGTTTCAGGCGCGGCGCCTGCAGGTGTTCGTGAAGCTCTGTGAAACACGGCACATGCAAACCGTGGCGCGGCACTTCGGcc                                                                                  <  2:157224/149‑1 (MQ=255)
                                                                cgcgTGCTGGCGGAACTGGACGGCGTGCCGAGTTTGCTGGGCACCGCGCAGGGCGAGCCCCTCTACCTGTTTCAGGCGCGGCGCCTGCAGGTGTTCGTGAAGCTCTGTGAAACACGGCACATGCAAACCGTGGCGCGGCACTTCGGCCt                                                                                 >  3:243949/1‑149 (MQ=255)
                                                                cgcgTGCTGGCGGAACTGGACGGCGTGCCGAGTTTGCTGGGCACCGCGCAGGGCGAGCCCCTCTACCTGTTTCAGGCGCGGCGCCTGCAGGTGTTCGTGAAGCTCTGTGAAACACGGCACATGCAAACCGTGGCGCGGCACTTCGGCCt                                                                                 >  7:78259/1‑149 (MQ=255)
                                                                   gTGCTGGCGGAACTGGACGGCGTGCCGAGTTTGCTGGGCACCGCGCAGGGCGAGCCCCTCTACCTGTTTCAGGCGCGGCGCCTGCAGGTGTTCGTGAAGCTCTGTGAAACACGGCACATGCAAACCGTGGCGCGGCACTTCGGCCTTag                                                                              <  4:243949/149‑1 (MQ=255)
                                                                                 ggACGGCGTGCCGAGTTTGCTGGGCACCGCGCAGGGCGAGCCCCTCTACCTGTTTCAGGCGCGGCGCCTGCAGGTGTTCGTGAAGCTCTGTGAAACACGGCACATGCAAACCGTGgcgc                                                                                              >  7:174378/1‑119 (MQ=255)
                                                                                 ggACGGCGTGCCGAGTTTGCTGGGCACCGCGCAGGGCGAGCCCCTCTACCTGTTTCAGGCGCGGCGCCTGCAGGTGTTCGTGAAGCTCTGTGAAACACGGCACATGCAAACCGTGgcgc                                                                                              <  8:174378/119‑1 (MQ=255)
                                                                                  gACGGCGTGCCGAGTTTGCTGGGCACCGCGCAGGGCGAGCCCCTCTACCTGTTTCAGGCGCGGCGCCTGCAGGTGTTCGTGAAGCTCTGTGAAAAACGGCACATGCAAACCGTGGCGCGGCACTTCGGCCTTAGCCAGCCGGCGGTCAg                                                               >  6:75671/1‑149 (MQ=255)
                                                                                    cGGCGTGCCGAGTTTGCTGGGCACCGCGCAGGGCGAGCCCCTCTACCTGTTTCAGGCGCGGCGCCTGCAGGTGTTCGTGAAGCTCTGTGAAACACGGCACATGCAAACCGTGGCGCGGCACTTCGGCCTTAGCCAGCCGGCGGTCAGtg                                                             <  5:260779/149‑1 (MQ=255)
                                                                                    cGGCGTGCCGAGTTTGCTGGGCACCGCGCAGGGCGAGCCCCTCTACCTGTTTCAGGCGCGGCGCCTGCAGGTGTTCGTGAAGCTCTGTGAAAAACGGCACATGCAAACCGTGGCGCGGCACTTCGGCCTTAGCCAGCCGGCGGTCAGtg                                                             <  5:75671/149‑1 (MQ=255)
                                                                                           ccGAGTTTGCTGGGCACCGCGCAGGGCGAGCCCCTCTACCTGTTTCAGGCGCGGCGCCTGCAGGTGTTCGTGAAGCTCTGTGAAACACGGCACATGCAAACCGTGGCGCGGCACTTCGGCCTTAGCCAGCCGGCGGTCAGtg                                                             >  2:72653/1‑142 (MQ=255)
                                                                                           ccGAGTTTGCTGGGCACCGCGCAGGGCGAGCCCCTCTACCTGTTTCAGGCGCGGCGCCTGCAGGTGTTCGTGAAGCTCTGTGAAACACGGCACATGCAAACCGTGGCGCGGCACTTCGGCCTTAGCCAGCCGGCGGTCAGtg                                                             <  1:72653/142‑1 (MQ=255)
                                                                                           ccGAGTTTGCTGGGCACCGCGCAGGGCGAGCCCCTCTACCTGTTTCAGGCGCGGCGCCTGCAGGTGTTCGTGAAGCTCTGTGAAACACGGCACATGCAAACCGTGGCGCGGCACTTCGGCCTTAGCCAGCCGGCGGTCAGTGCTGCttt                                                      >  4:72148/1‑149 (MQ=255)
                                                                                                   gCTGGGCACCGCGCAGGGCGAGCCCCTCTACCTGTTTCAGGCGCGGCGCCTGCAGGTGTTCGTGAAGCTCTGTGAAACACGGCACATGCAAACCGTGGCGCGGCACTTCGGCCTTAGCCAGCCGGCGGTCAGTGCTGCTTTGAAAGTg                                               <  3:323528/148‑1 (MQ=255)
                                                                                                    cTGGGCACCGCGCAGGGCGAGCCCCTCTACCTGTTTCAGGCGCGGCGCCTGCAGGTGTTCGTGAAGCTCTGTGAAACACGGCACATGCAAACCGTGGCGCGGCACTTCGGCCTTAGCCAGCCGGCGGTCAGTGCTGCTTTGAAAGTGCt                                             <  8:78259/149‑1 (MQ=255)
                                                                                                        gCACCGCGCAGGGCGAGCCCCTCTACCTGTTTCAGGCGCGGCGCCTGCAGGTGTTCGTGAAGCTCTGTGAAACACGGCACATGCAAACCGTGgcg                                                                                               <  8:161160/95‑1 (MQ=255)
                                                                                                        gCACCGCGCAGGGCGAGCCCCTCTACCTGTTTCAGGCGCGGCGCCTGCAGGTGTTCGTGAAGCTCTGTGAAACACGGCACATGCAAACCGTGgcg                                                                                               >  7:161160/1‑95 (MQ=255)
                                                                                                         cACCGCGCAGGGCGAGCCCCTCTACCTGTTTCAGGCGCGGCGCCTGCAGGTGTTCGTGAAGCTCTGTGAAACACGGCACATGCAAACCGTGGCGCGGCACTTCGGCCTTAGCCAGCCGGCGGTCAGTGCTGCTTTGAAAGTGCt                                             >  5:41088/1‑144 (MQ=255)
                                                                                                         cACCGCGCAGGGCGAGCCCCTCTACCTGTTTCAGGCGCGGCGCCTGCAGGTGTTCGTGAAGCTCTGTGAAACACGGCACATGCAAACCGTGGCGCGGCACTTCGGCCTTAGCCAGCCGGCGGTCAGTGCTGCTTTGAAAGTGCt                                             <  6:41088/144‑1 (MQ=255)
                                                                                                         cACCGCGCAGGGCGAGCCCCTCTACCTGTTTCAGGCGCGGCGCCTGCAGGTGTTCGTGAAGCTCTGTGAAACACGGCACATGCAAACCGTGGCGCGGCACTTCGGCCTTAGCCAGCCGGCGGTCAGTGCTGCTTTGAAAGTGCTCGAAg                                        >  7:146646/1‑149 (MQ=255)
                                                                                                               gcAGGGCGAGCCCCTCTACCTGTTTCAGGCGCGGCGCCTGCAGGTGTTCGTGAAGCTCTGTGAAACACGGCACATGCAAACCGTGGCGCGGCACTTCGGCCTTAGCCAGCCGGCGGTCAGTGCTGCTTTGAAAGTGCTCGAAGGCGGGt                                  <  5:36766/149‑1 (MQ=255)
                                                                                                                cAGGGCGAGCCCCTCTACCTGTTTCAGGCGCGGCGCCTGCAGGTGTTCGTGAAGCTCTGTGCAACACGGCACCTGCAACCCGTGGCGCGGCACTTCGGCCGTAGCCAGCCCGCGGTCAGTGCTGCTTTGAAAGGGCTCGAAGGCGGGTg                                 >  5:68104/1‑149 (MQ=255)
                                                                                                                 aGGGCGAGCCCCTCTACCTGTTTCAGGCGCGGCGCCTGCAGGTGTTTGTGAAGCTCTGTGAAACACGGCACATGCAAACCGTGGCGCGGCACTTCGGCCTTAGCCAGCCGGCGGTCAGTGCTGCTTTGAAAGTGCTCGAAGGCGGGTGc                                >  8:123501/1‑149 (MQ=255)
                                                                                                                 aGGGCGAGCCCCTCTACCTGTTTCAGGCGCGGCGCCTGCAGGTGTTCGTGAAGCTCTGTGAAACACGGCACATGCAAACCGTGGCGCGGCACTTCGGCCTTAGCCAGCCGGCGGTCAGTGCTGCTTTGAAAGTGCTCGAAGGCGGGTGc                                <  1:157239/149‑1 (MQ=255)
                                                                                                                      gAGCCCCTCTACCTGTTTCAGGCGCGGCGCCTGCAGGTGTTCGTGAAGCTCTGTGAAACACGGCACATGCAAACCGTGGCGCGGCACTTCGGCCTTAGCCAGCCGGCGGTCAGTGCTGCTTTGAAAGTGCTCGAAGGCGGGTGCGGa                             >  8:148250/1‑147 (MQ=255)
                                                                                                                           ccTCTACCTGTTTCAGGCGCGGCGCCTGCAGGTGTTCGTGAAGCTCTGTGAAACACGGCACATGCAAACCGTGGCGCGGCACTTCGGCCTTAGCCAGCCGGCGGt                                                                  <  6:39992/105‑1 (MQ=255)
                                                                                                                           ccTCTACCTGTTTCAGGCGCGGCGCCTGCAGGTGTTCGTGAAGCTCTGTGAAACACGGCACATGCAAACCGTGGCGCGGCACTTCGGCCTTAGCCAGCCGGCGGt                                                                  >  5:39992/1‑105 (MQ=255)
                                                                                                                            ctctACCTGTTTCAGGCGCGGCGCCTGCAGGTGTTCGTGAAGCTCTGTGAAACACGGCACATGCAAACCGTGGCGCGGCACTTCGGCCTTAGCCAGCCGGCGGTCAGTGCTGCTTTGAAAGTGc                                              <  7:154002/124‑1 (MQ=255)
                                                                                                                            ctctACCTGTTTCAGGCGCGGCGCCTGCAGGTGTTCGTGAAGCTCTGTGAAACACGGCACATGCAAACCGTGGCGCGGCACTTCGGCCTTAGCCAGCCGGCGGTCAGTGCTGCTTTGAAAGTGc                                              >  8:154002/1‑124 (MQ=255)
                                                                                                                                 ccTGTTTCAGGCGCGGCGCCTGCAGGTGTTCGTGAAGCTCTGTGAAACACGGCACATGCAAACCGTGGCGCGGCACTTCGGCCTTAGCCAGCCGGCGGTCAGTGCTGCTTTGAAAGTGCTCGAAGGCGGGTGCGGACAGCCCTTGTTCg                >  4:303843/1‑149 (MQ=255)
                                                                                                                                     ctgcAGGCGCCGCGCCTGCAGGTGTTCGTGAAGCTCTGTGAAACACGGCACATGCAAACCGTGGCGCGGCACTTCGGCCTTAGCCAGCCGGCGGTCAGTGCTGCTTTGAAAGTGCTCGAAGGCGGGTGCGGACAGCCCTTGTTCGTgcg            >  3:34959/4‑149 (MQ=255)
                                                                                                                                      ttCAGGCGCGGCGCCTGCAGGTGTTCGTGAAGCTCTGTGAAACACGGCACATGCAAACCGTGGCGCGGCACTTCGGCCTTAGCCAGCCGGCGGTCAGTGCTGCTTTGAAAGTGCTCGAAGGCGGGTGCGGACAGCCCTTGt                   >  7:113921/1‑141 (MQ=255)
                                                                                                                                      ttCAGGCGCGGCGCCTGCAGGTGTTCGTGAAGCTCTGTGAAACACGGCACATGCAAACCGTGGCGCGGCACTTCGGCCTTAGCCAGCCGGCGGTCAGTGCTGCTTTGAAAGTGCTCGAAGGCGGGTGCGGACAGCCCTTGt                   <  8:113921/141‑1 (MQ=255)
                                                                                                                                        cAGGCGCGGCGCCTGCAGGTGTTCGTGAAGCTCTGTGAAACACGGCACATGCAAACCGTGGCGCGGCACTTCGGCCTTAGCCAGCCGGCGGTCAGTGCTGCTTTGAAAGTGCTCGAAGGCGGGTGCGGACAGCCCTTGTTCGTGCGCAc         >  7:154479/1‑149 (MQ=255)
                                                                                                                                         aGGCGCCGCGCCTGCAGGTGTTCGTGAAGCTCTGTGAAACACGGCACATGCAAACCGTGGCGCGGCACTTCGGCCTTAGCCAGCCGGCGGTCAGTGCTGCTTTGAAAGTGCTCGAAGGCGGGTGCGGACAGCCCTTGTTCGTGCGCACg        <  4:34959/149‑1 (MQ=255)
                                                                                                                                           gcgcggcgcCTGCAGGTGTTCGTGAAGCTCTGTGAAACACGGCACATGCAAACCGTGGCGCGGCACTTCGGCCTTAGCCAGCCGGCGGTCAGTGCTGCTTTGAAAGTGCTCGAAGGCGGGTGCGGACAGCCCTTGTTCGTGCGCACGTc      >  6:3389/1‑149 (MQ=255)
                                                                                                                                               ggcgcCTGCAGGTGTTCGTGAAGCTCTGTGAAACACGGCACATGCAAACCGTGGCGCGGCACTTCGGCCTTAGCCAGCCGGCGGTCAGTGCTGCTTTGAAAGTGCTCGAAGGCGGGTGCGGACAGCCCTTGTTCGTGCGCACGTCGCGt  <  3:62414/149‑1 (MQ=255)
                                                                                                                                               |                                                                                                                                                    
CGAGCGCCATGCCAATGGCATGCGCCTGACCGACTATGGCGAGTGCCTGCTGCCGCGTGCACAGCGCGTGCTGGCGGAACTGGACGGCGTGCCGAGTTTGCTGGGCACCGCGCAGGGCGAGCCCCTCTACCTGTTTCAGGCGCGGCGCCTGCAGGTGTTCGTGAAGCTCTGTGAAACACGGCACATGCAAACCGTGGCGCGGCACTTCGGCCTTAGCCAGCCGGCGGTCAGTGCTGCTTTGAAAGTGCTCGAAGGCGGGTGCGGACAGCCCTTGTTCGTGCGCACGTCGCGT  >  NC_002947/2863102‑2863393

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑