Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,883,422 C→T 8.1% intergenic (+108/‑19) PP_2538 → / → PP_2539 hypothetical protein/LysR family transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,883,4220CT 8.1% 141.1 / 2.1 50intergenic (+108/‑19)PP_2538/PP_2539hypothetical protein/LysR family transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base C (20/25);  new base T (2/2);  total (23/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.91e-01

GCCTATGCCTGCCTGGCACGTCGTGCGCCGCGGTAAAACAGCCTGCAGAAAGGTGCGTGCATTCGTTCTGCAATCAAGGGTCATTTGCGCAAAACTCAACACGCCCCCGCAGACGACGCTCTATAGTGCTCGGCATGACGAAACCCCTGCCGAGC‑CTGCCTGATGATCCCTTCTCTGGACGCCATTTTTTCTCGCCTGCGCCTTCGCCAGTTGCGCTTGCTGATCGAGCTGGACCGCTGTGGCTCGCTGCATAAGGCAGCTGAAGCCATGGCCATTTCCCAGCCCGGT  >  NC_002947/2883279‑2883566
                                                                                                                                               |                                                                                                                                                 
gcctAAGCCTGCCTGGCACGTCGTGCGCCGCGGTAAAACAGCCTGCAGAAAGGTGCGTGCATTCGTTCTGCAATCAAGGGTCATTTGCGCAAAACTCAACACGCCCCCGCAGACGACGCTCTATAGTGCTCGGCATGACGAAACCCCTg                                                                                                                                              >  8:227114/1‑149 (MQ=255)
  ctATGCCTGCCTGGCACGTCGTGCGCCGCGGTAAAACAGCCTGCAGAAAGGTGCGTGCATTCGTTCTGCAATCAAGGGTCATTTGCGCAAAACTCAACACGCCCCCGCAGACGACGCTCTATAGTGCTCGGCATGACGAAACCCCTGcc                                                                                                                                            <  5:253905/149‑1 (MQ=255)
  ctATGCCTGCCTGGCACGTCGTGCGCCGCGGTAAAACAGCCTGCAGAAAGGTGCGTGCATTCGTTCTGCAATCAAGGGTCATTTGCGCAAAACTCAACACGCCCCCGCAGACGACGCTCTATAGTGCTCGGCATGACGAAACCCCTGcc                                                                                                                                            >  3:238419/1‑149 (MQ=255)
            ctgGCACGTCGTGCGCCGCGGTAAAACAGCCTGCAGAAAGGTGCGTGCATTCGTTCTGCAATCAAGGGTCATTTGCGCAAAACTCAACACGCCCCCGCAGACGACGCTCTATAGTGCTCGGCATGACGAAACCCCTGCCGAgc‑ctgcct                                                                                                                                 <  6:259000/149‑1 (MQ=255)
               gCACGTCGTGCGCCGCGGTAAAACAGCCTGCAGAAAGGTGCGTGCATTCGTTCTCCAATCAAGGGTCATTTGCGCAAAACTCAACACGCCCCCGCAGAAGACGCTCTATAGTGCTCTGCATGACGAACCCCCTGCCTCGC‑CAGCCtgat                                                                                                                              >  8:206171/1‑149 (MQ=255)
                cACGTCGTGCGCCGCGGTAAAACAGCCTGCAGAAAGGTGCGTGCATTCGTTCTGCAATCAAGGGTCATTTGCGCAAAACTCAACACGCCCCCGCAGACGACGCTCTATAGTGCTCGGCATGACGAAACCCCTGCCGAGC‑CTGCCtgatg                                                                                                                             <  4:238419/149‑1 (MQ=255)
                   gtcgtGCGCCGCGGTAAAACAGCCTGCAGAAAGGTGCGTGCATTCGTTCTGCAATCAAGGGTCATTTGCGCAAAACTCAACACGCCCCCGCAGACGACGCTCTATAGTGCTCGGCATGACGAAACCCCTGCCGAGC‑CTGCCTGATGATc                                                                                                                          >  1:170904/1‑149 (MQ=255)
                      gtGCGCCGCGGTAAAACAGCCTGCAGAAAGGTGCGTGCATTCGTTCTGCAATCAAGGGTCATTTGCGCAAAACTCAACACGCCCCCGCAGACGACGCTCTATAGTGCTCGGCATGACGAAACCCCTGCCGAGC‑CTGCCTGATGATccc                                                                                                                        <  7:10591/148‑1 (MQ=255)
                           ccgcTGTAAAACAGCCTGCAGAAAGGTGCGTGCATTCGTTCTGCAATCAAGGGTCATTTGCGCAAAACTCAACACGCCCCCGCAGACGACGCTCTATAGTGCTCGGCATGACGAAACCCCTGCCGAGC‑CTGCCTGATGATCCCTTctct                                                                                                                  <  4:237497/149‑1 (MQ=255)
                              cgGTAAAACAGCCTGCAGAAAGGTGCGTGCATTCGTTCTGCAATCAAGGGTCATTTGCGCAAAACTCAACACGCCCCCGCAGACGACGCTCTATAGTGCTCGGCATGACGAAACCCCTGCCGAGC‑CTGCCTGATGATCCCTTCTCTGGa                                                                                                               <  2:170904/149‑1 (MQ=255)
                                             cAGAAAGGTGCGTGCATTCGTTCTGCAATCAAGGGTCATTTGCGCAAAACTCAACACGCCCCCGCAGACGACGCTCTATAGTGCTCGGCATGACGAAACCCCTGCCGAGC‑CTGCCTGATGATccc                                                                                                                        <  8:53013/125‑1 (MQ=255)
                                             cAGAAAGGTGCGTGCATTCGTTCTGCAATCAAGGGTCATTTGCGCAAAACTCAACACGCCCCCGCAGACGACGCTCTATAGTGCTCGGCATGACGAAACCCCTGCCGAGC‑CTGCCTGATGATccc                                                                                                                        >  7:53013/1‑125 (MQ=255)
                                                 aaGGTGCGTGCATTCGTTCTGCAATCAAGGGTCATTTGCGCAAAACTCAACACGCCCCCGCAGACGACGCTCTATAGTGCTCGGCATGACGAAACCCCTGCCGAGC‑CTGCCTGATGATCCCTTCTCTGGACGCCATTTTTTCTCGCCTg                                                                                            >  2:27595/1‑149 (MQ=255)
                                                          gcATTCGTTCTGCAATCAAGGGTCATTTGCGCAAAACTCAACACGCCCCCGCAGACGACGCTCTATAGTGCTCGGCATGACGAAACCCCTGCCGAGC‑CTGCCTGATGATCCCTTCTCTGGACGCCATTTTTtct                                                                                                  <  2:782/134‑1 (MQ=255)
                                                          gcATTCGTTCTGCAATCAAGGGTCATTTGCGCAAAACTCAACACGCCCCCGCAGACGACGCTCTATAGTGCTCGGCATGACGAAACCCCTGCCGAGC‑CTGCCTGATGATCCCTTCTCTGGACGCCATTTTTtct                                                                                                  >  1:782/1‑134 (MQ=255)
                                                          gcATTCGTTCTGCAATCAAGGGTCATTTGCGCAAAACTCAACACGCCCCCGCAGACGACGCTCTATAGTGCTCGGCATGACGAAACCCCTGCCGAGC‑CTGCCTGATGATCCCTTCTCTGGACGCCATTTTTTCTCGCCTGCGCCTTCGc                                                                                   <  3:271454/149‑1 (MQ=255)
                                                           cATTCGTTCTGCAATCAAGGGTCATTTGCGCAAAACTCAACACGCCCCCGCAGACGACGCTCTATAGTGCTCGGCATGACGAAACCCCTGCCGAGC‑CTGCCTGATGATCCCTTCTCTGGACGCCATTTTTTCTCGCCTGCGCCTTCGc                                                                                   <  7:17643/148‑1 (MQ=255)
                                                           cATTCGTTCTGCAATCAAGGGTCATTTGCGCAAAACTCAACACGCCCCCGCAGACGACGCTCTACAGTGATCGGCATGACGAACCCCCTGCCGAGC‑CTGCCTGATGATCCCTTCTCTGGACGCCATTTTTTCTCGCCTGCGCCTTCGc                                                                                   >  1:133259/1‑148 (MQ=255)
                                                                gTTCTGCAATCAAGGGTCATTTGCGCAAAACTCAACACGCCCCCGCAGACGACGCTCTATAGTGCTCGGCATGACGAAACCCCTGCCGAGC‑CTGCCTGATGATCCCTTCTCTGGACGCCATTTTTTCTCGCCTGCGCCTTCGCCAGTTg                                                                             >  2:268632/1‑149 (MQ=255)
                                                                 ttCTGCAATCAAGGGTCATTTGCGCAAAACTCAACACGCCCCCGCAGACGACGCTCTATAGTGCTCGGCATGACGAAACCCCTGCCGAGC‑CTGCCTGATGATCCCTTCTCTGGACGCCATTTTTTCTCGCCTGCGCCTTCGCCAGTTgc                                                                            <  8:267436/149‑1 (MQ=255)
                                                                 ttCTGCAATCAAGGGTCATTTGCGCAAAACTCAACACGCCCCCGCAGACGACGCTCTATAGTGCTCGGCATGACGAAACCCCTGCCGAGC‑CTGCCTGATGATCCCTTCTCTGGACGCCATTTTTTCTCGCCTGCGCCTTCGCCAGTTgc                                                                            >  5:216579/1‑149 (MQ=255)
                                                                           aaGGGTCATTTGCGCAAAACTCAACACGCCCCCGCAGACGACGCTCTATAGTGCTCGGCATGACGAAACACCTGCCGAGC‑CTGCCTGATGATCCCTTCTCTGGACGCCATTTTTTCTCGCCTGCGCCTTCGCCAGTTGCGCTTGCTGAt                                                                  >  5:16694/1‑149 (MQ=255)
                                                                            aGGGTCATTTGCGCAAAACTCAACATGCCCCCGCAGACGACGCTCTATAGTGCTCGGCATGACGAAACCCCTGCCGAGC‑CTGCCTGATGATCCCTTCTCTGGACGCCATTTTTTCTCGCCTGCGCCTTCGCCAGTTGCGCTTGCTGATc                                                                 >  3:223834/1‑149 (MQ=255)
                                                                             gggTCATTTGCGCAAAACTCAACACGCCCCCGCAGACGACGCTCTATAGTGCTCGGCATGACGAAATCCCTGCCGAGC‑CTGCCTGATGATCCCTTCTCTGGACGCCATTTTTTCTCGCCTGCGCCTTCGCCAGTTGCGCTTGCTGATc                                                                 >  2:39228/1‑148 (MQ=255)
                                                                             gggTCATTTGCGCAAAACTCAACACGCCCCCGCAGACGACGCTCTATAGTGCTCGGCATGACGAAATCCCTGCCGAGC‑CTGCCTGATGATCCCTTCTCTGGACGCCATTTTTTCTCGCCTGCGCCTTCGCCAGTTGCGCTTGCTGATc                                                                 >  2:44296/1‑148 (MQ=255)
                                                                               gTCATTTGCGCAAAACTCAACACGCCCCCGCAGACGACGCTCTATAGTGCTCGGCATGACGAAACCCCTGCCGAGC‑CTGCCTGATGATCCCTTCTCTGGACGCCATTTTTTCTCGCCTGCGCCTTCGCCAGTTGCGCTTGCTGATCGAg                                                              <  7:227114/149‑1 (MQ=255)
                                                                                tCATTTGCGCAAAACTCAACACGCCCCCGCAGACGACGCTCTATAGTGCTCGGCATGACGAAACCCCTGCCGAGC‑CTGCCTGATGATCCCTTCTCTGGACGCCATTTTTTCTCGCCTGCGCCTTCGCCAGTTGCGCTTGc                                                                      <  5:21282/140‑1 (MQ=255)
                                                                                tCATTTGCGCAAAACTCAACACGCCCCCGCAGACGACGCTCTATAGTGCTCGGCATGACGAAACCCCTGCCGAGC‑CTGCCTGATGATCCCTTCTCTGGACGCCATTTTTTCTCGCCTGCGCCTTCGCCAGTTGCGCTTGc                                                                      >  6:21282/1‑140 (MQ=255)
                                                                                            aaCTCAACACGCCCCCGCAGACGACGCTCTATAGTGCTCGGCATGACGAAACCCCTGCCGAGC‑CTGCCTGATGATCCCTTCTCTGGACGCCATTTTTTCTCGCCTGCGCCTTCGCCAGTTGCGCTTGCTGATCGAGCTGGACCGCtgtg                                                 <  2:225039/149‑1 (MQ=255)
                                                                                             aCTCAACACGCCCCCGCAGACGACGCTCTATAGTGCTCGGCATGACGAATCCCCTGCCGAGC‑CTGCCTGATGATCCCTTCTCTGGACGCCATTTTTTCTCGCCTGCGCCTTCGCCAGTTGCGCTTGCTGATCGAGCTGGACCGCTGTgg                                                <  6:140485/149‑1 (MQ=255)
                                                                                             aCTCAACACGCCCCCGCAGACGACGCTCTATAGTGCTCGGCATGACGAAACCCCTGCCGAGC‑CTGCCTGATGATCCCTTCTCTGGACGCCATTTTTTCTCGCCTGCGCCTTCGCCAGTTGCGCTTGCTGATCGAGCTGGa                                                         <  8:175186/140‑1 (MQ=255)
                                                                                             aCTCAACACGCCCCCGCAGACGACGCTCTATAGTGCTCGGCATGACGAAACCCCTGCCGAGC‑CTGCCTGATGATCCCTTCTCTGGACGCCATTTTTTCTCGCCTGCGCCTTCGCCAGTTGCGCTTGCTGATCGAGCTGGa                                                         >  7:175186/1‑140 (MQ=255)
                                                                                              cTCAACACGCCCCCGCAGACGACGCTCTATAGTGCTCGGCATGACGAAACCCCTGCCGAGC‑CTGCCTGATGATCCCTTCTCTGGACGCCATTTTTTCTCGCCTGCGCCTTCGCCAGTTGCGCTTGCTGATCGAGCTGGACCGCTGTGGc                                               >  8:192198/1‑149 (MQ=255)
                                                                                               tCAACACGCCCCCGCAGACGACGCTCTATAGTGCTCGGCATGACGAAACCCCTGCCGAGCGCTGCCTGATGATCCCTTCTCTGGACGCCATTTTTTCTCGCCTGCGCCTTCGCCAGTTGCGCTTGCTGATCGAGCTGGACCGCTGTGGc                                               >  1:267844/1‑149 (MQ=255)
                                                                                                      gCCCCCGCAGACGACGCTCTATAGTGCTCGGCATGACGAAACCCCTGCCGAGC‑CTGCCTGATGATCCCTTCTCTGGACGCCATTTTTTCTCGCCTGCGCCTTCGCCAGTTGCGCTTGCTGATCGAGCTGGACCGCTGTGGCTCGCTGCa                                       >  4:37396/1‑149 (MQ=255)
                                                                                                        ccccGCAGACGACGCTCTATAGTGCTCGGCATGACGAAACCCCTGCCGAGC‑CTGCCAGATGATCCCTTCTCTGGACGCCATTTTTTCTCGCCTGCGCCTTCGCCAGTTGCGCTTGCTGATCGAGCTGGACCGCTGTGGCTCGCTGCAt                                      >  4:241207/1‑148 (MQ=255)
                                                                                                         cccGCAGACGACGCTCTATAGTGCTAGGCATGACGAATCCCCTGCCGAGC‑CTGCCTGATGATCCCTTCTCTGGACGCCATTTTTTCTCGCCTGCGCCTTCGCCAGTTGCGCTTGATGATCGAGCTGGACCGCTGTGGCTCGCTGCATaa                                    <  1:330148/149‑1 (MQ=255)
                                                                                                          ccGCAGACGACGCTCTATAGTGCTCGGCATGACGAAACCCCTGCCGAGC‑CTGCCTGATGATCCCTTCTCTGGACGCCATTTTTTCTCGCCTGCGCCTTCGCCAGTTGCGCTTGCTGATCGAGCTGGACCGCTGTGGCTCGCTGCATAAg                                   <  1:268632/149‑1 (MQ=255)
                                                                                                              aGACGACGCTCTATAGTGCTCGGCATGACGAAACCCCTGCCGAGC‑CTGCCTGATGATCCCTTCTCTGGACGCCATTTTTTCTCGCCTGCGCCTTCGCCAGTTGCGCTTGc                                                                      <  4:180264/110‑1 (MQ=255)
                                                                                                              aGACGACGCTCTATAGTGCTCGGCATGACGAAACCCCTGCCGAGC‑CTGCCTGATGATCCCTTCTCTGGACGCCATTTTTTCTCGCCTGCGCCTTCGCCAGTTGCGCTTGc                                                                      >  3:180264/1‑110 (MQ=255)
                                                                                                                cctatcttctATAGTGCTCGGCATGAAGAAACCCCTGCCGAGC‑CTGCCTTATGATACCTTCTCTGGACGCCATTTTTTCTCGCCTGCGCCTTCGCCAGTTGCGCTTGCTGATCGAGCTGGACCTCTGTTGCTAGCTGAATAAGGCAGc                              <  2:199962/141‑1 (MQ=255)
                                                                                                                   acgCTCTATAGTGCTCGGCATGACGAAACCCCTGCCGAGC‑CTGCCTGATGATCCCTTCTCTGGACGCCATTTTTTCTCGCCTGCGCCTTCGCCAGTTGCGCTTGCTGATCGAGCTGGACCGCTGTGGCTCGCTGCATAAGGCAGCTGaa                          <  4:223834/149‑1 (MQ=255)
                                                                                                                   acgCTCTATAGTGCTCGGCATGACGAAACCCCTGCCGAGC‑CTGCCTGATGATCCCTTCTCTGGACGCCATTTTTTCTCGCCTGCGCCTTCGCCAGTTGCGCTTGCTGATCGAGCTGGACCGCTGTGGCTCGCTGCATAAGGCAGCTGaa                          <  2:267844/149‑1 (MQ=255)
                                                                                                                        ctATAGTGCTCGGCATGACGAAAAACCTGCAGAGC‑CTGCCTGATGATACCTTCTCTGGACGCCATTTTTTCTCGAATGCGCCTTCGCCAGTTGCGCTTTCTGATCGAGCTGGACCGCTGTGGCTCGCTGCATAATGCAGCtatatcgg                      >  1:199962/1‑141 (MQ=255)
                                                                                                                           taGTGCTAGGGATGACGAAACGCCTGCCGAGC‑CTGCCTGATGATCCCTTCTCTGGACGCCATTTTTTCTCGCCTGCGCCTTCGCCAGTTGCGCTTGCTGATCGAGCTTGACCGCTGTGGCTCGCTGCATAAGGCAGCTGAAGCCATgg                   <  7:206171/148‑1 (MQ=255)
                                                                                                                                cTCGGCATGACGAAACCCCTGCCGAGC‑CTGCCTGATGATCCCTTCTCTGGACGCCATTTTTTCTCGCCTGCGCCTTCGCCAGTTGCGCTTGCTGATCGAGCTGGACCGCTGTGGCTCGCTGCATAAGGCAGCTGAAGCCATGGCCAttt             <  1:77722/149‑1 (MQ=255)
                                                                                                                                        gACGAAACCCCTGCCGAGC‑CTGCCTGATGATCCCTTCTCTGGACGCCATTTTTTCTCGCCTGCGCCTTCGCCAGTTGCGCTTGCTGATCGAGCTGGACCGCTGTGGCTCGCTGCATAAGGCAGCTGAAGCCATGGCCATTTCCCAGccc     >  6:65528/1‑149 (MQ=255)
                                                                                                                                         aCGATACCCCTGCCGAGG‑CTGCCTGATGATCCCTTCTCTGGACGCCATTTTTTCTCGCCTGCGCCTTCGCCTGTTGCGCTTGCTGATCGAGCTGGACCGCTGTGGCTCGCTGCATAAGCCAGCTGAAGCCATGGCCAttt             <  3:286325/140‑1 (MQ=255)
                                                                                                                                           gAAATCCCTGCCGAGC‑CTGCCTGATGATCCCTTCTCTGGACGCCATTTTTTCTCGCCTGCGCCTTCGCCAGTTGCGCTTGCTGATCGAGCTGGACCGCTGTGGCTCGCTGCATAAGGCAGCTGAAGCCATGGCCATTTCCCAGCCCGGt  <  1:39228/149‑1 (MQ=255)
                                                                                                                                           gAAATCCCTGCCGAGC‑CTGCCTGATGATCCCTTCTCTGGACGCCATTTTTTCTCGCCTGCGCCTTCGCCAGTTGCGCTTGCTGATCGAGCTGGACCGCTGTGGCTCGCTGCATAAGGCAGCTGAAGCCATGGCCATTTCCCAGCCCGGt  <  1:44296/149‑1 (MQ=255)
                                                                                                                                               |                                                                                                                                                 
GCCTATGCCTGCCTGGCACGTCGTGCGCCGCGGTAAAACAGCCTGCAGAAAGGTGCGTGCATTCGTTCTGCAATCAAGGGTCATTTGCGCAAAACTCAACACGCCCCCGCAGACGACGCTCTATAGTGCTCGGCATGACGAAACCCCTGCCGAGC‑CTGCCTGATGATCCCTTCTCTGGACGCCATTTTTTCTCGCCTGCGCCTTCGCCAGTTGCGCTTGCTGATCGAGCTGGACCGCTGTGGCTCGCTGCATAAGGCAGCTGAAGCCATGGCCATTTCCCAGCCCGGT  >  NC_002947/2883279‑2883566

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑