Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,887,552 G→A 9.0% L373L (CTG→TTG)  PP_2542 ← GntR family transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,887,5520GA 9.0% 124.4 / 2.3 45L373L (CTG→TTG) PP_2542GntR family transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base G (19/21);  new base A (2/2);  total (22/23)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.00e-01

ATCGTCCAGCAACGCGGCACTGTCGGGAATCGCCGGATGGCGGGTCCAGATGTACATGCCTTCATCCGATTCGGTGAACAATTCAAAGCCCAGTCGATGCAGATGCCGGCCAACTTCCTGATGCGCCTCGGCCAGGCGCTGGCGCAGGCGCTTGAGGTGCTTGCG‑CCAGCGCCCGTCGATGATCGCCGCATACACCACGCGCTCCATCACCTGCGAGGTGGTCAGGCCTGAGCGCATCTTCAGGTGCAGCAGTTTTTGCATCAGTTCGGGGTTGGC  >  NC_002947/2887406‑2887681
                                                                                                                                                  |                                                                                                                                  
aTCGTCCAGCAACGCGGCACTGTCGGGAATCGCCGGATGGCGGGTCCAGATGTACATGCCTTCATCCGATTCGGTGAACAATTCAAAGCCCAGTCGATGCAGATGCCGGCCAACTTCCTGATGCGCCTCGGCCAGGCGCTGGCGCAGgc                                                                                                                                  <  5:61509/149‑1 (MQ=255)
      cAGCAACGCGGCACTGTCGGGAATCGCCGGATGGCGGGTCCAGATGTACATGCCTTCATCCGATTCGGTGAACAATTCAAAGCCCAGTCGATGCAGATGCCGGCCAACTTCCTGATGCGCCTCGGCCAGGCGCTGGCGCAGGCGCtt                                                                                                                              >  6:179184/1‑147 (MQ=255)
       aGCAACGCGGCACTGTCGGGAATCGCCGGATGGCGGGTCCAGATGTACATGCCTTCATCCGATTCGGTGAACAATTCAAAGCCCAGTCGATGCAGATGCCGGCCAACTTCCTGATGCGCCTCGGCCAGGCGCTGGTGCAGGCGCTTGAg                                                                                                                           >  2:304109/1‑149 (MQ=255)
              cgGCACTGTCGGGAATCGCCGGATGGCGGGTCCAGATGTACATGCCTTCATCCGATTCGGTGAACAATTCAAAGCCCAGTCGATGCAGATGCCGGCCAACTTCCTGATGCGCCTCGGCCAGGCGCTGGCGCAGGCGCTTGAGGTGCTTg                                                                                                                    >  4:188576/1‑149 (MQ=255)
                   cTGTCGGGAATCGCCGGATGGCGGGTCCAGATGTACATGCCTTCATCCGATTCGGTGAACAATTCAAAGCCCAGTCGATGCAGATGCCGGCCAACTTCCTGATGCGCCTCGGCCAGGCGCTGGCGCAGGCGCTTGAGGTGCTTGCG‑CCa                                                                                                              <  8:117149/149‑1 (MQ=255)
                      tCGGGAATCGCCGGATGGCGGGTCCAGATGTACATGCCTTCATCCGATTCGGTGAACAATTCAAAGCCCAGTCGATGCAGATGCCGGCCAACTTCCTGATGCGCCTCGGCCAGGCGCTGGCGCAGGCGCTTGAGGTGCTTGCG‑CCAgcg                                                                                                           <  6:82741/149‑1 (MQ=255)
                         ggAATCGCCGGATGGCGGGTCCAGATGTACATGCCTTCATCCGATTCGGTGAACAATTCAAAGCCCAGTCGATGCAGATGCCGGCCAACTTCCTGATGCGCCTCGGCCAGGCGCTGGCGCAGGCGCTTGAGGTGCTTGCG‑CCAGCGccc                                                                                                        <  5:179184/149‑1 (MQ=255)
                          gAATCGCCGGATGGCGGGTCCAGATGTACATGCCTTCATCCGATTCGGTGAACAATTCAAAGCCCAGTCGATGCAGATGCCGGCCAACTTCCTGATGCGCCTCGGCCAGGCGCTGGCGCAAGCACCTCAAGCGCCTGCG‑CCAgc                                                                                                            <  1:116575/144‑1 (MQ=38)
                          gAATCGCCGGATGGCGGGTCCAGATGTACATGCCTTCATCCGATTCGGTGAACAATTCAAAGCCCAGTCGATGCAGATGCCGGCCAACTTCCTGATGCGCCTCGGCCAGGCGCTGGCGCAAGCACCTCAAGCGCCTGCG‑CCAgc                                                                                                            >  2:116575/1‑144 (MQ=38)
                           aaTCGCCGGATGGCGGGTCCAGATGTACATGCCTTCATCCGATTCGGTGAACAATTCAAAGCCCAGTCGATGCAGATGCCGGCCAACTTCCTGATGCGCCTCGGCCAGGCGCTGGTGCAGGCGCTTGAGGTGCTTGCG‑CCAGCGCCCGt                                                                                                      <  1:304109/149‑1 (MQ=255)
                           aaTCGCCGGATGGCGGGTCCAGATGTACATGCCTTCATCCGATTCGGTGAACAATTCAAAGCCCAGTCGATGCAGATGCCGGCCAACTTCCTGATGCGCCTCGGCCAGGCGCTGGCGCAGGCGCTTGAGGTGCTTGCG‑CCAGCGCCCGt                                                                                                      <  3:188576/149‑1 (MQ=255)
                                   gATGGCGGGTCCCGTTGTACATGCCTTCATCCGCTGCGGTGAACATTTCAAAGCCCAGGCGATGCTGATGCCGGCCAACTTCCTGATGCGCCTCGGCCTGGCGCTGGCGCTGGCGCTTGAGGTGCTTGCG‑CCAGCGCCCGTCGATGATc                                                                                              <  5:329006/149‑1 (MQ=255)
                                     tGGCGGGTCCAGATGTACATGCCTTCATCCGATTCGGTGAACAATTCAAAGCCCAGTCGATGCAGATGCCGGCCAACTTCCTGATGCGCCTCGGCCAGGCGCTGGCGCAGGCGCTTGAGGTGCTTGCG‑CCAGCGCCCGTCGATGATcgc                                                                                            >  6:133389/1‑149 (MQ=255)
                                              cAGATGTACTTGCCTTCATCCGATTCGGTGAACAATTCAAAGCCCAGTCGATGCAGATGCCGGCCAACTTCCTGATGCGCCTCGGCCAGGCGCTGGCGCAGGCGCTTGAGGTGCTTGCG‑CCAGCGCCCGTCGATGATCGCCGCATacac                                                                                   <  5:161400/149‑1 (MQ=255)
                                               aGATGTACATGCCTTCATCCGATTCGGTGAACAATTCAAAGCCCAGTCGATGCAGATGCCGGCCAACTTCCTGATGCGCCTCGGCCAGGCGCTGGCGCCGGCGCTTGAGGTGCTTGCG‑CCAGCGCCCGTCGATGCTCGCCGCATacac                                                                                   >  2:314999/1‑148 (MQ=255)
                                                    tACATGCCTTCATCCGATTCGGTGAACAATTCAAAGCCCAGTCGATGCAGATGCCGGCCAACTTCCTGATGCGCCTCGGCCAGGCGCTGGCGCAGGCGCTTGAGGTGCTTGCG‑CCAGCGCCCGTCGATGATCGCCGCATACACCAcgcg                                                                             >  1:122026/1‑149 (MQ=255)
                                                     aCATGCCTTCATCCGATTCGGTGAACAATTCAAAGCCCAGTCGATGCAGATGCCGGCCAACTTCCTGATGCGCCTCGGCCAGGCGCTGGCGCAGGCGCTTGAGGTGCTTGCG‑CCAGCGCCCGTCGATGATCGCCGCATACACCACgcgc                                                                            >  6:313619/1‑149 (MQ=255)
                                                       aTGCCTTCATCCGATTCGGTGAACAATTCAAAGCCCAGTCGATGCAGATGCCGGCCAACTTCCTGATGCGCCTCGGCCAGGCGCTGGCGCAGGCGCTTGAGGTGCTTGCG‑CCAGCGCCCGTCGATGATCGCCGCATACACCACGCGCTc                                                                          <  5:313619/149‑1 (MQ=255)
                                                       aTGCCTTCATCCGATTCGGTGAACAATTCAAAGCCCAGTCGATGCAGATGCCGGCCAACTTCCTGATGCGCCTCGGCCAGGCGCTGGCGCAGGCGCTTGAGGTGCTTGCG‑CCAGCGCCCGTCGATGATAGCCGCATACACCACGCGCTc                                                                          >  5:204804/1‑149 (MQ=255)
                                                         gCCTTCATCCGATTCGGTGAACAATTCAAAGCCCAGTCGATGCAGATGCCGGCCAACTTCCTGATGCGCCTCGGCCAGGCGCTGGCGCAGGCGCTTGAGGTGCTTGCG‑CCAGCGCCCGTCGATGATCGCCGCATACACCACGCGCTCCa                                                                        >  3:240258/1‑149 (MQ=255)
                                                                 ccGATTCGGTGAACAATTCAAAGCCCAGTCGATGCAGATGCCGGCAAACTTCCTGATGCGCCTCGGCCAGGCGCTGGCGCAGGCGCTTGAGGTGCTTGCG‑CCAGCGCCCGTCGATGATCCACGCATACACCACGCTCTACATCACCTGc                                                                >  8:195231/1‑149 (MQ=255)
                                                                  gttttCGGTTAACATTTCAACGCCCAGTCGTTGCAGATGCCGGCCAACTTCCTGATGCGCCTCGGCCAGGCGCTGGCGCAGGCGGTTGAGGTGCTTGCG‑CCAGCGCCCGTCGATGATCGCCGCATACACCACGCGCTCCATCACCTGc                                                                <  7:195231/145‑1 (MQ=255)
                                                                   gATTCGGTGAACAATTCAAAGCCCAGTCGATGCAGATGCCGGCCAACTTCCTGATGCGCCTCGGCCAGGCGCTGGCGCAGGCGCTTGAGGTGCTTGCG‑CCAGCGCCCGTCGATGATCGCCGCATACACCACGCGCTCCATCACCTGc                                                                >  4:220775/1‑147 (MQ=255)
                                                                               atttCAACGCCCCGTGGATGCTGATGCCGGCCAACTTCCTGATGCGCGTGGGCCTGGGGCTGGGGCCGGGGGTTTAGGTGCTGGCG‑CCTGCGCCCGTCGATGATCGCCGCATACACCACGCGCTCCATCACCTGCGAGGTGGTCAGGc                                                   <  8:92227/146‑1 (MQ=255)
                                                                                aTTCAAAGCCCAGTCGATGCAGATGCCGGCCAACTTCCTGATGCGCCTCGGCCAGGCGCTGGCGCAGGCGCTTGAGGTGCTTGCG‑CCAGCGCCCGTCGATGATCGCCGCATACACCACGCGCTCCATCACCTGCGAGGTGGTCAGGc                                                   >  7:92227/1‑147 (MQ=255)
                                                                                aTTCAAAGCCCAGTCGATGCAGATGCCGGCCAACTTCCTGATGCGCCTCGGCCAGGCGCTGGCGCAGGCGCTTGAGGTGCTTGCG‑CCAGCGCCCGTCGATGATCGCCGCATACACCACGCGCTCCATCACCTGCGAGGTGGTCAGGCCt                                                 >  7:138687/1‑149 (MQ=255)
                                                                                    aaaGCCCAGTCGATGCAGATGCCGGCCAACTTCCTGATGCGCCTCGGCCAGGCGCTGGCGCAGGCGCTTGAGGTGCTTGCG‑CCAGCGCCCGTCGATGATCGCCGCATACACCACGCGCTCCATCACCTGCGAGGTGGTCAGGCCTGAg                                              <  3:220775/148‑1 (MQ=255)
                                                                                        cccAGTCGATGCAGATGCCGGCCAACTTCCTGATGCGCCTCGGCCAGGCGCTGGCGCAGGCGCTTGAGGTGCTTgcg‑c                                                                                                                >  2:191372/1‑78 (MQ=255)
                                                                                        cccAGTCGATGCAGATGCCGGCCAACTTCCTGATGCGCCTCGGCCAGGCCCTGGCGCAGGCGCTTGAGGTGCTTgcg‑c                                                                                                                <  1:191372/78‑1 (MQ=255)
                                                                                          cAGTCGATGCAGATGCCGGCCAACTTCCTGATGCGCCTCGGCCAGGCGCTGGCGCAGGCGCTTGAGGTGCTTGCG‑CCAGCGCCCGTCGATGATCGCCGCATACACCACGCGCTCCATCACCTGCGAGGTGGTCAGGCCTGAGCGCATCt                                       >  3:24954/1‑149 (MQ=255)
                                                                                             tctaTGCATAGGCCGGCAAATTTCCTGATGCGCCTCGGCCAGGCGGTGGCGCTGGCGGTTGAGGTGCTTGCG‑CCAGCGCCCGTCGATGATCGCCGCATACACCACGCGCTCCATCACCTGCGAGGTGGTCAGGCCTGAGCGCATCTTc                                     <  8:138687/145‑1 (MQ=255)
                                                                                                 tGCAGATGCCGGCCAACTTCCTGATGCGCCTCGGCCAGGCGCTGGCGCAGGCGCTTGAGGTGCTTGCG‑CCAGCGCCCGTCGATGATCGCCGCATACACCACGCGCTCCATCACCTGCGAGGTGGTCAGGCCTGAGCGCATCTTCAg                                   <  4:24954/146‑1 (MQ=255)
                                                                                                           ggCCAACTTCCTGATGCGCCTCGGCCAGGCGCTGGCGCAGGCGCTTGAGGTGCTTGCG‑CCAGCGCCCGTCGATGATCGCCGCATACACCACGCGCTCCATCACCTGCGAGGTGGTCAGGCCTGAGCGCATCTTCAGGTGCAGCAGtttt                      >  1:58906/1‑149 (MQ=255)
                                                                                                            gCCATCTTCCTGATGCGCCTGGGCCAGGCGCTGGCTCAGGCGCTTGAGGTGCTTGCG‑CAAGCGCCCGTCGATGATCGCCGCATACACCACGCGCTCCATCACCTGCGAGGTGGTCAGGCCTGAGCGCATCTTCAGGTGCAGCAGttttt                     <  4:240258/149‑1 (MQ=255)
                                                                                                             ccAACTTCCTGATGCGCCTCGGCCAGGCGCTGGCGCAGGCGCTTGAGGTGCTTGCG‑CCAGCGCCCGTCGATGATCGCCGCATACACCACGCGCTCCATCACCTGCGAg                                                             <  7:150144/108‑1 (MQ=255)
                                                                                                             ccAACTTCCTGATGCGCCTCGGCCAGGCGCTGGCGCAGGCGCTTGAGGTGCTTGCG‑CCAGCGCCCGTCGATGATCGCCGCATACACCACGCGCTCCATCACCTGCGAg                                                             >  8:150144/1‑108 (MQ=255)
                                                                                                             caaaCTTCCTGATGCGCCTCGGCCAGGCGCTGGCGCGGGCGCTTGCGGTGCTTGCG‑CCAGCGCCCGTCGATGATCGCCGCATACACCACGCGCTCCATCACCTGCGAGGTGGTCAGGCCTGAGCGCATCTTCAGGTGCAGCAGTTTTTg                    <  3:276159/147‑1 (MQ=255)
                                                                                                                 cTTCCTGATGCGCCTCGGCCAGGCGCTGGCGCAGGCGCTTGAGGTGCTTGCG‑CCAGCGCCCGTCGATGATCGCCGCATACACCACGCGCTCCATCACCTGCGAGGTGGTCAGGCCTGAGCGCATCTTCAGGTGCAGCAGTTTTTGCATc                <  5:133389/149‑1 (MQ=255)
                                                                                                                            gcCTCGGCCAGGCGCTGGCGCAGGCGCTTGAGGTGCTTGCG‑CCAGCGCCCGTCGATGATCGCCGCATACACCACGCGCTCCATCACCTGCGAGGTGGTCAGGCCTGAGCGCATCTTCAGGTGCAGCAGTTTTTGCATCAGTTCGGGGtt     <  2:58906/149‑1 (MQ=255)
                                                                                                                              cTCGGCCAGGCGCTGGCGCACGCGCTTGACGTGCTTGCG‑CAAGCGCCCGTCGATGATCGCCGCATACAACACGCGCTCCATAACCTCCGAGGTTGACACGCCTGACCGCATCTTCAGGTGCAAAAGTTTTTGCATAAGTTAGGCGTTgt   >  2:300168/1‑148 (MQ=255)
                                                                                                                               tCGGCCAGGCGCTCGCGCAGGCGCTTGAGGTGCTTGCG‑CCAGCGCCCGTCGATGATCGCCGCATACACCACGCGCTCCATCACCTGCGAGGTGGTCAGGCCGGAGCGCATCTTCAGGTGCAGCAGTTTTTGCATCAGTTCGGGGTTGGc  >  7:123450/1‑149 (MQ=255)
                                                                                                                                     cggCGCGGGCGCTGGCGCTTGTGGTGCTTGCG‑CCAGCGCCCGTCGATGATCGCCGCATACACCACGCGCTCCATCACCTGCGAGGTGGTCAGGCCTGAGCGCATCTTCAGGTGCAGCAGTTTTTGCATCAGTTCGGGGTTg    <  2:184723/140‑1 (MQ=255)
                                                                                                                                     aGGCGCTGGCGCAGGCGCTTGAGGTGCTTGCG‑CCAGCGCCCGTCGATGATCGCCGCATACACCACGCGCTCCATCACCTGCGAGGTGGTCAGGCCTGAGCGCATCTTCAGGTGCAGCAGTTTTTGCATCAGTTCGGGGTTg    >  1:184723/1‑141 (MQ=255)
                                                                                                                                         gcTGGCGCAAGCACCTCAAGCGCCTGCGTCCAGCGCCCGTCGATGATCGCCGCATACACCACGCGCTcc                                                                         >  1:21063/1‑69 (MQ=12)
                                                                                                                                         gcTGGCGCAAGCACCTCAAGCGCCTGCG‑CCAGCGCCCGTCGATGATCGCCGCATACACCACGCGCTcc                                                                         <  2:21063/68‑1 (MQ=17)
                                                                                                                                                  |                                                                                                                                  
ATCGTCCAGCAACGCGGCACTGTCGGGAATCGCCGGATGGCGGGTCCAGATGTACATGCCTTCATCCGATTCGGTGAACAATTCAAAGCCCAGTCGATGCAGATGCCGGCCAACTTCCTGATGCGCCTCGGCCAGGCGCTGGCGCAGGCGCTTGAGGTGCTTGCG‑CCAGCGCCCGTCGATGATCGCCGCATACACCACGCGCTCCATCACCTGCGAGGTGGTCAGGCCTGAGCGCATCTTCAGGTGCAGCAGTTTTTGCATCAGTTCGGGGTTGGC  >  NC_002947/2887406‑2887681

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑