Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,887,557 T→C 9.3% K371R (AAG→AGG)  PP_2542 ← GntR family transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,887,5570TC 9.3% 118.6 / 3.2 43K371R (AAG→AGG) PP_2542GntR family transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base T (19/20);  new base C (2/2);  total (21/22)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.03e-01

CAGCAACGCGGCACTGTCGGGAATCGCCGGATGGCGGGTCCAGATGTACATGCCTTCATCCGATTCGGTGAACAATTCAAAGCCCAGTCGATGCAGATGCCGGCCAACTTCCTGATGCGCCTCGGCCAGGCGCTGGCGCAGGCGCTTGAGGTGCTTGCG‑CCAGCGCCCGTCGATGATCGCCGCATACACCACGCGCTCCATCACCTGCGAGGTGGTCAGGCCTGAGCGCATCTTCAGGTGCAGCAGTTTTTGCATCAGTTCGGGGTTGGC  >  NC_002947/2887412‑2887681
                                                                                                                                                 |                                                                                                                             
cAGCAACGCGGCACTGTCGGGAATCGCCGGATGGCGGGTCCAGATGTACATGCCTTCATCCGATTCGGTGAACAATTCAAAGCCCAGTCGATGCAGATGCCGGCCAACTTCCTGATGCGCCTCGGCCAGGCGCTGGCGCAGGCGCtt                                                                                                                              >  6:179184/1‑147 (MQ=255)
 aGCAACGCGGCACTGTCGGGAATCGCCGGATGGCGGGTCCAGATGTACATGCCTTCATCCGATTCGGTGAACAATTCAAAGCCCAGTCGATGCAGATGCCGGCCAACTTCCTGATGCGCCTCGGCCAGGCGCTGGTGCAGGCGCTTGAg                                                                                                                           >  2:304109/1‑149 (MQ=255)
        cgGCACTGTCGGGAATCGCCGGATGGCGGGTCCAGATGTACATGCCTTCATCCGATTCGGTGAACAATTCAAAGCCCAGTCGATGCAGATGCCGGCCAACTTCCTGATGCGCCTCGGCCAGGCGCTGGCGCAGGCGCTTGAGGTGCTTg                                                                                                                    >  4:188576/1‑149 (MQ=255)
             cTGTCGGGAATCGCCGGATGGCGGGTCCAGATGTACATGCCTTCATCCGATTCGGTGAACAATTCAAAGCCCAGTCGATGCAGATGCCGGCCAACTTCCTGATGCGCCTCGGCCAGGCGCTGGCGCAGGCGCTTGAGGTGCTTGCG‑CCa                                                                                                              <  8:117149/149‑1 (MQ=255)
                tCGGGAATCGCCGGATGGCGGGTCCAGATGTACATGCCTTCATCCGATTCGGTGAACAATTCAAAGCCCAGTCGATGCAGATGCCGGCCAACTTCCTGATGCGCCTCGGCCAGGCGCTGGCGCAGGCGCTTGAGGTGCTTGCG‑CCAgcg                                                                                                           <  6:82741/149‑1 (MQ=255)
                   ggAATCGCCGGATGGCGGGTCCAGATGTACATGCCTTCATCCGATTCGGTGAACAATTCAAAGCCCAGTCGATGCAGATGCCGGCCAACTTCCTGATGCGCCTCGGCCAGGCGCTGGCGCAGGCGCTTGAGGTGCTTGCG‑CCAGCGccc                                                                                                        <  5:179184/149‑1 (MQ=255)
                    gAATCGCCGGATGGCGGGTCCAGATGTACATGCCTTCATCCGATTCGGTGAACAATTCAAAGCCCAGTCGATGCAGATGCCGGCCAACTTCCTGATGCGCCTCGGCCAGGCGCTGGCGCAAGCACCTCAAGCGCCTGCG‑CCAgc                                                                                                            <  1:116575/144‑1 (MQ=38)
                    gAATCGCCGGATGGCGGGTCCAGATGTACATGCCTTCATCCGATTCGGTGAACAATTCAAAGCCCAGTCGATGCAGATGCCGGCCAACTTCCTGATGCGCCTCGGCCAGGCGCTGGCGCAAGCACCTCAAGCGCCTGCG‑CCAgc                                                                                                            >  2:116575/1‑144 (MQ=38)
                     aaTCGCCGGATGGCGGGTCCAGATGTACATGCCTTCATCCGATTCGGTGAACAATTCAAAGCCCAGTCGATGCAGATGCCGGCCAACTTCCTGATGCGCCTCGGCCAGGCGCTGGTGCAGGCGCTTGAGGTGCTTGCG‑CCAGCGCCCGt                                                                                                      <  1:304109/149‑1 (MQ=255)
                     aaTCGCCGGATGGCGGGTCCAGATGTACATGCCTTCATCCGATTCGGTGAACAATTCAAAGCCCAGTCGATGCAGATGCCGGCCAACTTCCTGATGCGCCTCGGCCAGGCGCTGGCGCAGGCGCTTGAGGTGCTTGCG‑CCAGCGCCCGt                                                                                                      <  3:188576/149‑1 (MQ=255)
                             gATGGCGGGTCCCGTTGTACATGCCTTCATCCGCTGCGGTGAACATTTCAAAGCCCAGGCGATGCTGATGCCGGCCAACTTCCTGATGCGCCTCGGCCTGGCGCTGGCGCTGGCGCTTGAGGTGCTTGCG‑CCAGCGCCCGTCGATGATc                                                                                              <  5:329006/149‑1 (MQ=255)
                               tGGCGGGTCCAGATGTACATGCCTTCATCCGATTCGGTGAACAATTCAAAGCCCAGTCGATGCAGATGCCGGCCAACTTCCTGATGCGCCTCGGCCAGGCGCTGGCGCAGGCGCTTGAGGTGCTTGCG‑CCAGCGCCCGTCGATGATcgc                                                                                            >  6:133389/1‑149 (MQ=255)
                                        cAGATGTACTTGCCTTCATCCGATTCGGTGAACAATTCAAAGCCCAGTCGATGCAGATGCCGGCCAACTTCCTGATGCGCCTCGGCCAGGCGCTGGCGCAGGCGCTTGAGGTGCTTGCG‑CCAGCGCCCGTCGATGATCGCCGCATacac                                                                                   <  5:161400/149‑1 (MQ=255)
                                         aGATGTACATGCCTTCATCCGATTCGGTGAACAATTCAAAGCCCAGTCGATGCAGATGCCGGCCAACTTCCTGATGCGCCTCGGCCAGGCGCTGGCGCCGGCGCTTGAGGTGCTTGCG‑CCAGCGCCCGTCGATGCTCGCCGCATacac                                                                                   >  2:314999/1‑148 (MQ=255)
                                              tACATGCCTTCATCCGATTCGGTGAACAATTCAAAGCCCAGTCGATGCAGATGCCGGCCAACTTCCTGATGCGCCTCGGCCAGGCGCTGGCGCAGGCGCTTGAGGTGCTTGCG‑CCAGCGCCCGTCGATGATCGCCGCATACACCAcgcg                                                                             >  1:122026/1‑149 (MQ=255)
                                               aCATGCCTTCATCCGATTCGGTGAACAATTCAAAGCCCAGTCGATGCAGATGCCGGCCAACTTCCTGATGCGCCTCGGCCAGGCGCTGGCGCAGGCGCTTGAGGTGCTTGCG‑CCAGCGCCCGTCGATGATCGCCGCATACACCACgcgc                                                                            >  6:313619/1‑149 (MQ=255)
                                                 aTGCCTTCATCCGATTCGGTGAACAATTCAAAGCCCAGTCGATGCAGATGCCGGCCAACTTCCTGATGCGCCTCGGCCAGGCGCTGGCGCAGGCGCTTGAGGTGCTTGCG‑CCAGCGCCCGTCGATGATCGCCGCATACACCACGCGCTc                                                                          <  5:313619/149‑1 (MQ=255)
                                                 aTGCCTTCATCCGATTCGGTGAACAATTCAAAGCCCAGTCGATGCAGATGCCGGCCAACTTCCTGATGCGCCTCGGCCAGGCGCTGGCGCAGGCGCTTGAGGTGCTTGCG‑CCAGCGCCCGTCGATGATAGCCGCATACACCACGCGCTc                                                                          >  5:204804/1‑149 (MQ=255)
                                                   gCCTTCATCCGATTCGGTGAACAATTCAAAGCCCAGTCGATGCAGATGCCGGCCAACTTCCTGATGCGCCTCGGCCAGGCGCTGGCGCAGGCGCTTGAGGTGCTTGCG‑CCAGCGCCCGTCGATGATCGCCGCATACACCACGCGCTCCa                                                                        >  3:240258/1‑149 (MQ=255)
                                                           ccGATTCGGTGAACAATTCAAAGCCCAGTCGATGCAGATGCCGGCAAACTTCCTGATGCGCCTCGGCCAGGCGCTGGCGCAGGCGCTTGAGGTGCTTGCG‑CCAGCGCCCGTCGATGATCCACGCATACACCACGCTCTACATCACCTGc                                                                >  8:195231/1‑149 (MQ=255)
                                                            gttttCGGTTAACATTTCAACGCCCAGTCGTTGCAGATGCCGGCCAACTTCCTGATGCGCCTCGGCCAGGCGCTGGCGCAGGCGGTTGAGGTGCTTGCG‑CCAGCGCCCGTCGATGATCGCCGCATACACCACGCGCTCCATCACCTGc                                                                <  7:195231/145‑1 (MQ=255)
                                                             gATTCGGTGAACAATTCAAAGCCCAGTCGATGCAGATGCCGGCCAACTTCCTGATGCGCCTCGGCCAGGCGCTGGCGCAGGCGCTTGAGGTGCTTGCG‑CCAGCGCCCGTCGATGATCGCCGCATACACCACGCGCTCCATCACCTGc                                                                >  4:220775/1‑147 (MQ=255)
                                                                         atttCAACGCCCCGTGGATGCTGATGCCGGCCAACTTCCTGATGCGCGTGGGCCTGGGGCTGGGGCCGGGGGTTTAGGTGCTGGCG‑CCTGCGCCCGTCGATGATCGCCGCATACACCACGCGCTCCATCACCTGCGAGGTGGTCAGGc                                                   <  8:92227/146‑1 (MQ=255)
                                                                          aTTCAAAGCCCAGTCGATGCAGATGCCGGCCAACTTCCTGATGCGCCTCGGCCAGGCGCTGGCGCAGGCGCTTGAGGTGCTTGCG‑CCAGCGCCCGTCGATGATCGCCGCATACACCACGCGCTCCATCACCTGCGAGGTGGTCAGGc                                                   >  7:92227/1‑147 (MQ=255)
                                                                          aTTCAAAGCCCAGTCGATGCAGATGCCGGCCAACTTCCTGATGCGCCTCGGCCAGGCGCTGGCGCAGGCGCTTGAGGTGCTTGCG‑CCAGCGCCCGTCGATGATCGCCGCATACACCACGCGCTCCATCACCTGCGAGGTGGTCAGGCCt                                                 >  7:138687/1‑149 (MQ=255)
                                                                              aaaGCCCAGTCGATGCAGATGCCGGCCAACTTCCTGATGCGCCTCGGCCAGGCGCTGGCGCAGGCGCTTGAGGTGCTTGCG‑CCAGCGCCCGTCGATGATCGCCGCATACACCACGCGCTCCATCACCTGCGAGGTGGTCAGGCCTGAg                                              <  3:220775/148‑1 (MQ=255)
                                                                                  cccAGTCGATGCAGATGCCGGCCAACTTCCTGATGCGCCTCGGCCAGGCGCTGGCGCAGGCGCTTGAGGTGCTTgcg‑c                                                                                                                >  2:191372/1‑78 (MQ=255)
                                                                                  cccAGTCGATGCAGATGCCGGCCAACTTCCTGATGCGCCTCGGCCAGGCCCTGGCGCAGGCGCTTGAGGTGCTTgcg‑c                                                                                                                <  1:191372/78‑1 (MQ=255)
                                                                                    cAGTCGATGCAGATGCCGGCCAACTTCCTGATGCGCCTCGGCCAGGCGCTGGCGCAGGCGCTTGAGGTGCTTGCG‑CCAGCGCCCGTCGATGATCGCCGCATACACCACGCGCTCCATCACCTGCGAGGTGGTCAGGCCTGAGCGCATCt                                       >  3:24954/1‑149 (MQ=255)
                                                                                       tctaTGCATAGGCCGGCAAATTTCCTGATGCGCCTCGGCCAGGCGGTGGCGCTGGCGGTTGAGGTGCTTGCG‑CCAGCGCCCGTCGATGATCGCCGCATACACCACGCGCTCCATCACCTGCGAGGTGGTCAGGCCTGAGCGCATCTTc                                     <  8:138687/145‑1 (MQ=255)
                                                                                           tGCAGATGCCGGCCAACTTCCTGATGCGCCTCGGCCAGGCGCTGGCGCAGGCGCTTGAGGTGCTTGCG‑CCAGCGCCCGTCGATGATCGCCGCATACACCACGCGCTCCATCACCTGCGAGGTGGTCAGGCCTGAGCGCATCTTCAg                                   <  4:24954/146‑1 (MQ=255)
                                                                                                     ggCCAACTTCCTGATGCGCCTCGGCCAGGCGCTGGCGCAGGCGCTTGAGGTGCTTGCG‑CCAGCGCCCGTCGATGATCGCCGCATACACCACGCGCTCCATCACCTGCGAGGTGGTCAGGCCTGAGCGCATCTTCAGGTGCAGCAGtttt                      >  1:58906/1‑149 (MQ=255)
                                                                                                      gCCATCTTCCTGATGCGCCTGGGCCAGGCGCTGGCTCAGGCGCTTGAGGTGCTTGCG‑CAAGCGCCCGTCGATGATCGCCGCATACACCACGCGCTCCATCACCTGCGAGGTGGTCAGGCCTGAGCGCATCTTCAGGTGCAGCAGttttt                     <  4:240258/149‑1 (MQ=255)
                                                                                                       ccAACTTCCTGATGCGCCTCGGCCAGGCGCTGGCGCAGGCGCTTGAGGTGCTTGCG‑CCAGCGCCCGTCGATGATCGCCGCATACACCACGCGCTCCATCACCTGCGAg                                                             <  7:150144/108‑1 (MQ=255)
                                                                                                       ccAACTTCCTGATGCGCCTCGGCCAGGCGCTGGCGCAGGCGCTTGAGGTGCTTGCG‑CCAGCGCCCGTCGATGATCGCCGCATACACCACGCGCTCCATCACCTGCGAg                                                             >  8:150144/1‑108 (MQ=255)
                                                                                                       caaaCTTCCTGATGCGCCTCGGCCAGGCGCTGGCGCGGGCGCTTGCGGTGCTTGCG‑CCAGCGCCCGTCGATGATCGCCGCATACACCACGCGCTCCATCACCTGCGAGGTGGTCAGGCCTGAGCGCATCTTCAGGTGCAGCAGTTTTTg                    <  3:276159/147‑1 (MQ=255)
                                                                                                           cTTCCTGATGCGCCTCGGCCAGGCGCTGGCGCAGGCGCTTGAGGTGCTTGCG‑CCAGCGCCCGTCGATGATCGCCGCATACACCACGCGCTCCATCACCTGCGAGGTGGTCAGGCCTGAGCGCATCTTCAGGTGCAGCAGTTTTTGCATc                <  5:133389/149‑1 (MQ=255)
                                                                                                                      gcCTCGGCCAGGCGCTGGCGCAGGCGCTTGAGGTGCTTGCG‑CCAGCGCCCGTCGATGATCGCCGCATACACCACGCGCTCCATCACCTGCGAGGTGGTCAGGCCTGAGCGCATCTTCAGGTGCAGCAGTTTTTGCATCAGTTCGGGGtt     <  2:58906/149‑1 (MQ=255)
                                                                                                                        cTCGGCCAGGCGCTGGCGCACGCGCTTGACGTGCTTGCG‑CAAGCGCCCGTCGATGATCGCCGCATACAACACGCGCTCCATAACCTCCGAGGTTGACACGCCTGACCGCATCTTCAGGTGCAAAAGTTTTTGCATAAGTTAGGCGTTgt   >  2:300168/1‑148 (MQ=255)
                                                                                                                         tCGGCCAGGCGCTCGCGCAGGCGCTTGAGGTGCTTGCG‑CCAGCGCCCGTCGATGATCGCCGCATACACCACGCGCTCCATCACCTGCGAGGTGGTCAGGCCGGAGCGCATCTTCAGGTGCAGCAGTTTTTGCATCAGTTCGGGGTTGGc  >  7:123450/1‑149 (MQ=255)
                                                                                                                               cggCGCGGGCGCTGGCGCTTGTGGTGCTTGCG‑CCAGCGCCCGTCGATGATCGCCGCATACACCACGCGCTCCATCACCTGCGAGGTGGTCAGGCCTGAGCGCATCTTCAGGTGCAGCAGTTTTTGCATCAGTTCGGGGTTg    <  2:184723/140‑1 (MQ=255)
                                                                                                                               aGGCGCTGGCGCAGGCGCTTGAGGTGCTTGCG‑CCAGCGCCCGTCGATGATCGCCGCATACACCACGCGCTCCATCACCTGCGAGGTGGTCAGGCCTGAGCGCATCTTCAGGTGCAGCAGTTTTTGCATCAGTTCGGGGTTg    >  1:184723/1‑141 (MQ=255)
                                                                                                                                   gcTGGCGCAAGCACCTCAAGCGCCTGCGTCCAGCGCCCGTCGATGATCGCCGCATACACCACGCGCTcc                                                                         >  1:21063/1‑69 (MQ=12)
                                                                                                                                   gcTGGCGCAAGCACCTCAAGCGCCTGCG‑CCAGCGCCCGTCGATGATCGCCGCATACACCACGCGCTcc                                                                         <  2:21063/68‑1 (MQ=17)
                                                                                                                                                 |                                                                                                                             
CAGCAACGCGGCACTGTCGGGAATCGCCGGATGGCGGGTCCAGATGTACATGCCTTCATCCGATTCGGTGAACAATTCAAAGCCCAGTCGATGCAGATGCCGGCCAACTTCCTGATGCGCCTCGGCCAGGCGCTGGCGCAGGCGCTTGAGGTGCTTGCG‑CCAGCGCCCGTCGATGATCGCCGCATACACCACGCGCTCCATCACCTGCGAGGTGGTCAGGCCTGAGCGCATCTTCAGGTGCAGCAGTTTTTGCATCAGTTCGGGGTTGGC  >  NC_002947/2887412‑2887681

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑