Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,887,559 G→C 9.3% L370L (CTC→CTG) ‡ PP_2542 ← GntR family transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,887,5590GC 9.3% 119.6 / 4.1 43L370L (CTC→CTG) ‡PP_2542GntR family transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base G (19/19);  new base C (2/2);  total (21/22)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.74e-01

AGCAACGCGGCACTGTCGGGAATCGCCGGATGGCGGGTCCAGATGTACATGCCTTCATCCGATTCGGTGAACAATTCAAAGCCCAGTCGATGCAGATGCCGGCCAACTTCCTGATGCGCCTCGGCCAGGCGCTGGCGCAGGCGCTTGAGGTGCTTGCG‑CCAGCGCCCGTCGATGATCGCCGCATACACCACGCGCTCCATCACCTGCGAGGTGGTCAGGCCTGAGCGCATCTTCAGGTGCAGCAGTTTTTGCATCAGTTCGGGGTTGGCCAGCATGTAGCCGACCCGCA  >  NC_002947/2887413‑2887701
                                                                                                                                                  |                                                                                                                                               
aGCAACGCGGCACTGTCGGGAATCGCCGGATGGCGGGTCCAGATGTACATGCCTTCATCCGATTCGGTGAACAATTCAAAGCCCAGTCGATGCAGATGCCGGCCAACTTCCTGATGCGCCTCGGCCAGGCGCTGGTGCAGGCGCTTGAg                                                                                                                                               >  2:304109/1‑149 (MQ=255)
       cgGCACTGTCGGGAATCGCCGGATGGCGGGTCCAGATGTACATGCCTTCATCCGATTCGGTGAACAATTCAAAGCCCAGTCGATGCAGATGCCGGCCAACTTCCTGATGCGCCTCGGCCAGGCGCTGGCGCAGGCGCTTGAGGTGCTTg                                                                                                                                        >  4:188576/1‑149 (MQ=255)
            cTGTCGGGAATCGCCGGATGGCGGGTCCAGATGTACATGCCTTCATCCGATTCGGTGAACAATTCAAAGCCCAGTCGATGCAGATGCCGGCCAACTTCCTGATGCGCCTCGGCCAGGCGCTGGCGCAGGCGCTTGAGGTGCTTGCG‑CCa                                                                                                                                  <  8:117149/149‑1 (MQ=255)
               tCGGGAATCGCCGGATGGCGGGTCCAGATGTACATGCCTTCATCCGATTCGGTGAACAATTCAAAGCCCAGTCGATGCAGATGCCGGCCAACTTCCTGATGCGCCTCGGCCAGGCGCTGGCGCAGGCGCTTGAGGTGCTTGCG‑CCAgcg                                                                                                                               <  6:82741/149‑1 (MQ=255)
                  ggAATCGCCGGATGGCGGGTCCAGATGTACATGCCTTCATCCGATTCGGTGAACAATTCAAAGCCCAGTCGATGCAGATGCCGGCCAACTTCCTGATGCGCCTCGGCCAGGCGCTGGCGCAGGCGCTTGAGGTGCTTGCG‑CCAGCGccc                                                                                                                            <  5:179184/149‑1 (MQ=255)
                   gAATCGCCGGATGGCGGGTCCAGATGTACATGCCTTCATCCGATTCGGTGAACAATTCAAAGCCCAGTCGATGCAGATGCCGGCCAACTTCCTGATGCGCCTCGGCCAGGCGCTGGCGCAAGCACCTCAAGCGCCTGCG‑CCAgc                                                                                                                                <  1:116575/144‑1 (MQ=38)
                   gAATCGCCGGATGGCGGGTCCAGATGTACATGCCTTCATCCGATTCGGTGAACAATTCAAAGCCCAGTCGATGCAGATGCCGGCCAACTTCCTGATGCGCCTCGGCCAGGCGCTGGCGCAAGCACCTCAAGCGCCTGCG‑CCAgc                                                                                                                                >  2:116575/1‑144 (MQ=38)
                    aaTCGCCGGATGGCGGGTCCAGATGTACATGCCTTCATCCGATTCGGTGAACAATTCAAAGCCCAGTCGATGCAGATGCCGGCCAACTTCCTGATGCGCCTCGGCCAGGCGCTGGTGCAGGCGCTTGAGGTGCTTGCG‑CCAGCGCCCGt                                                                                                                          <  1:304109/149‑1 (MQ=255)
                    aaTCGCCGGATGGCGGGTCCAGATGTACATGCCTTCATCCGATTCGGTGAACAATTCAAAGCCCAGTCGATGCAGATGCCGGCCAACTTCCTGATGCGCCTCGGCCAGGCGCTGGCGCAGGCGCTTGAGGTGCTTGCG‑CCAGCGCCCGt                                                                                                                          <  3:188576/149‑1 (MQ=255)
                            gATGGCGGGTCCCGTTGTACATGCCTTCATCCGCTGCGGTGAACATTTCAAAGCCCAGGCGATGCTGATGCCGGCCAACTTCCTGATGCGCCTCGGCCTGGCGCTGGCGCTGGCGCTTGAGGTGCTTGCG‑CCAGCGCCCGTCGATGATc                                                                                                                  <  5:329006/149‑1 (MQ=255)
                              tGGCGGGTCCAGATGTACATGCCTTCATCCGATTCGGTGAACAATTCAAAGCCCAGTCGATGCAGATGCCGGCCAACTTCCTGATGCGCCTCGGCCAGGCGCTGGCGCAGGCGCTTGAGGTGCTTGCG‑CCAGCGCCCGTCGATGATcgc                                                                                                                >  6:133389/1‑149 (MQ=255)
                                       cAGATGTACTTGCCTTCATCCGATTCGGTGAACAATTCAAAGCCCAGTCGATGCAGATGCCGGCCAACTTCCTGATGCGCCTCGGCCAGGCGCTGGCGCAGGCGCTTGAGGTGCTTGCG‑CCAGCGCCCGTCGATGATCGCCGCATacac                                                                                                       <  5:161400/149‑1 (MQ=255)
                                        aGATGTACATGCCTTCATCCGATTCGGTGAACAATTCAAAGCCCAGTCGATGCAGATGCCGGCCAACTTCCTGATGCGCCTCGGCCAGGCGCTGGCGCCGGCGCTTGAGGTGCTTGCG‑CCAGCGCCCGTCGATGCTCGCCGCATacac                                                                                                       >  2:314999/1‑148 (MQ=255)
                                             tACATGCCTTCATCCGATTCGGTGAACAATTCAAAGCCCAGTCGATGCAGATGCCGGCCAACTTCCTGATGCGCCTCGGCCAGGCGCTGGCGCAGGCGCTTGAGGTGCTTGCG‑CCAGCGCCCGTCGATGATCGCCGCATACACCAcgcg                                                                                                 >  1:122026/1‑149 (MQ=255)
                                              aCATGCCTTCATCCGATTCGGTGAACAATTCAAAGCCCAGTCGATGCAGATGCCGGCCAACTTCCTGATGCGCCTCGGCCAGGCGCTGGCGCAGGCGCTTGAGGTGCTTGCG‑CCAGCGCCCGTCGATGATCGCCGCATACACCACgcgc                                                                                                >  6:313619/1‑149 (MQ=255)
                                                aTGCCTTCATCCGATTCGGTGAACAATTCAAAGCCCAGTCGATGCAGATGCCGGCCAACTTCCTGATGCGCCTCGGCCAGGCGCTGGCGCAGGCGCTTGAGGTGCTTGCG‑CCAGCGCCCGTCGATGATCGCCGCATACACCACGCGCTc                                                                                              <  5:313619/149‑1 (MQ=255)
                                                aTGCCTTCATCCGATTCGGTGAACAATTCAAAGCCCAGTCGATGCAGATGCCGGCCAACTTCCTGATGCGCCTCGGCCAGGCGCTGGCGCAGGCGCTTGAGGTGCTTGCG‑CCAGCGCCCGTCGATGATAGCCGCATACACCACGCGCTc                                                                                              >  5:204804/1‑149 (MQ=255)
                                                  gCCTTCATCCGATTCGGTGAACAATTCAAAGCCCAGTCGATGCAGATGCCGGCCAACTTCCTGATGCGCCTCGGCCAGGCGCTGGCGCAGGCGCTTGAGGTGCTTGCG‑CCAGCGCCCGTCGATGATCGCCGCATACACCACGCGCTCCa                                                                                            >  3:240258/1‑149 (MQ=255)
                                                          ccGATTCGGTGAACAATTCAAAGCCCAGTCGATGCAGATGCCGGCAAACTTCCTGATGCGCCTCGGCCAGGCGCTGGCGCAGGCGCTTGAGGTGCTTGCG‑CCAGCGCCCGTCGATGATCCACGCATACACCACGCTCTACATCACCTGc                                                                                    >  8:195231/1‑149 (MQ=255)
                                                           gttttCGGTTAACATTTCAACGCCCAGTCGTTGCAGATGCCGGCCAACTTCCTGATGCGCCTCGGCCAGGCGCTGGCGCAGGCGGTTGAGGTGCTTGCG‑CCAGCGCCCGTCGATGATCGCCGCATACACCACGCGCTCCATCACCTGc                                                                                    <  7:195231/145‑1 (MQ=255)
                                                            gATTCGGTGAACAATTCAAAGCCCAGTCGATGCAGATGCCGGCCAACTTCCTGATGCGCCTCGGCCAGGCGCTGGCGCAGGCGCTTGAGGTGCTTGCG‑CCAGCGCCCGTCGATGATCGCCGCATACACCACGCGCTCCATCACCTGc                                                                                    >  4:220775/1‑147 (MQ=255)
                                                                        atttCAACGCCCCGTGGATGCTGATGCCGGCCAACTTCCTGATGCGCGTGGGCCTGGGGCTGGGGCCGGGGGTTTAGGTGCTGGCG‑CCTGCGCCCGTCGATGATCGCCGCATACACCACGCGCTCCATCACCTGCGAGGTGGTCAGGc                                                                       <  8:92227/146‑1 (MQ=255)
                                                                         aTTCAAAGCCCAGTCGATGCAGATGCCGGCCAACTTCCTGATGCGCCTCGGCCAGGCGCTGGCGCAGGCGCTTGAGGTGCTTGCG‑CCAGCGCCCGTCGATGATCGCCGCATACACCACGCGCTCCATCACCTGCGAGGTGGTCAGGc                                                                       >  7:92227/1‑147 (MQ=255)
                                                                         aTTCAAAGCCCAGTCGATGCAGATGCCGGCCAACTTCCTGATGCGCCTCGGCCAGGCGCTGGCGCAGGCGCTTGAGGTGCTTGCG‑CCAGCGCCCGTCGATGATCGCCGCATACACCACGCGCTCCATCACCTGCGAGGTGGTCAGGCCt                                                                     >  7:138687/1‑149 (MQ=255)
                                                                             aaaGCCCAGTCGATGCAGATGCCGGCCAACTTCCTGATGCGCCTCGGCCAGGCGCTGGCGCAGGCGCTTGAGGTGCTTGCG‑CCAGCGCCCGTCGATGATCGCCGCATACACCACGCGCTCCATCACCTGCGAGGTGGTCAGGCCTGAg                                                                  <  3:220775/148‑1 (MQ=255)
                                                                                 cccAGTCGATGCAGATGCCGGCCAACTTCCTGATGCGCCTCGGCCAGGCGCTGGCGCAGGCGCTTGAGGTGCTTgcg‑c                                                                                                                                    >  2:191372/1‑78 (MQ=255)
                                                                                 cccAGTCGATGCAGATGCCGGCCAACTTCCTGATGCGCCTCGGCCAGGCCCTGGCGCAGGCGCTTGAGGTGCTTgcg‑c                                                                                                                                    <  1:191372/78‑1 (MQ=255)
                                                                                   cAGTCGATGCAGATGCCGGCCAACTTCCTGATGCGCCTCGGCCAGGCGCTGGCGCAGGCGCTTGAGGTGCTTGCG‑CCAGCGCCCGTCGATGATCGCCGCATACACCACGCGCTCCATCACCTGCGAGGTGGTCAGGCCTGAGCGCATCt                                                           >  3:24954/1‑149 (MQ=255)
                                                                                      tctaTGCATAGGCCGGCAAATTTCCTGATGCGCCTCGGCCAGGCGGTGGCGCTGGCGGTTGAGGTGCTTGCG‑CCAGCGCCCGTCGATGATCGCCGCATACACCACGCGCTCCATCACCTGCGAGGTGGTCAGGCCTGAGCGCATCTTc                                                         <  8:138687/145‑1 (MQ=255)
                                                                                          tGCAGATGCCGGCCAACTTCCTGATGCGCCTCGGCCAGGCGCTGGCGCAGGCGCTTGAGGTGCTTGCG‑CCAGCGCCCGTCGATGATCGCCGCATACACCACGCGCTCCATCACCTGCGAGGTGGTCAGGCCTGAGCGCATCTTCAg                                                       <  4:24954/146‑1 (MQ=255)
                                                                                                    ggCCAACTTCCTGATGCGCCTCGGCCAGGCGCTGGCGCAGGCGCTTGAGGTGCTTGCG‑CCAGCGCCCGTCGATGATCGCCGCATACACCACGCGCTCCATCACCTGCGAGGTGGTCAGGCCTGAGCGCATCTTCAGGTGCAGCAGtttt                                          >  1:58906/1‑149 (MQ=255)
                                                                                                     gCCATCTTCCTGATGCGCCTGGGCCAGGCGCTGGCTCAGGCGCTTGAGGTGCTTGCG‑CAAGCGCCCGTCGATGATCGCCGCATACACCACGCGCTCCATCACCTGCGAGGTGGTCAGGCCTGAGCGCATCTTCAGGTGCAGCAGttttt                                         <  4:240258/149‑1 (MQ=255)
                                                                                                      ccAACTTCCTGATGCGCCTCGGCCAGGCGCTGGCGCAGGCGCTTGAGGTGCTTGCG‑CCAGCGCCCGTCGATGATCGCCGCATACACCACGCGCTCCATCACCTGCGAg                                                                                 >  8:150144/1‑108 (MQ=255)
                                                                                                      ccAACTTCCTGATGCGCCTCGGCCAGGCGCTGGCGCAGGCGCTTGAGGTGCTTGCG‑CCAGCGCCCGTCGATGATCGCCGCATACACCACGCGCTCCATCACCTGCGAg                                                                                 <  7:150144/108‑1 (MQ=255)
                                                                                                      caaaCTTCCTGATGCGCCTCGGCCAGGCGCTGGCGCGGGCGCTTGCGGTGCTTGCG‑CCAGCGCCCGTCGATGATCGCCGCATACACCACGCGCTCCATCACCTGCGAGGTGGTCAGGCCTGAGCGCATCTTCAGGTGCAGCAGTTTTTg                                        <  3:276159/147‑1 (MQ=255)
                                                                                                          cTTCCTGATGCGCCTCGGCCAGGCGCTGGCGCAGGCGCTTGAGGTGCTTGCG‑CCAGCGCCCGTCGATGATCGCCGCATACACCACGCGCTCCATCACCTGCGAGGTGGTCAGGCCTGAGCGCATCTTCAGGTGCAGCAGTTTTTGCATc                                    <  5:133389/149‑1 (MQ=255)
                                                                                                                     gcCTCGGCCAGGCGCTGGCGCAGGCGCTTGAGGTGCTTGCG‑CCAGCGCCCGTCGATGATCGCCGCATACACCACGCGCTCCATCACCTGCGAGGTGGTCAGGCCTGAGCGCATCTTCAGGTGCAGCAGTTTTTGCATCAGTTCGGGGtt                         <  2:58906/149‑1 (MQ=255)
                                                                                                                       cTCGGCCAGGCGCTGGCGCACGCGCTTGACGTGCTTGCG‑CAAGCGCCCGTCGATGATCGCCGCATACAACACGCGCTCCATAACCTCCGAGGTTGACACGCCTGACCGCATCTTCAGGTGCAAAAGTTTTTGCATAAGTTAGGCGTTgt                       >  2:300168/1‑148 (MQ=255)
                                                                                                                        tCGGCCAGGCGCTCGCGCAGGCGCTTGAGGTGCTTGCG‑CCAGCGCCCGTCGATGATCGCCGCATACACCACGCGCTCCATCACCTGCGAGGTGGTCAGGCCGGAGCGCATCTTCAGGTGCAGCAGTTTTTGCATCAGTTCGGGGTTGGc                      >  7:123450/1‑149 (MQ=255)
                                                                                                                              cggCGCGGGCGCTGGCGCTTGTGGTGCTTGCG‑CCAGCGCCCGTCGATGATCGCCGCATACACCACGCGCTCCATCACCTGCGAGGTGGTCAGGCCTGAGCGCATCTTCAGGTGCAGCAGTTTTTGCATCAGTTCGGGGTTg                        <  2:184723/140‑1 (MQ=255)
                                                                                                                              aGGCGCTGGCGCAGGCGCTTGAGGTGCTTGCG‑CCAGCGCCCGTCGATGATCGCCGCATACACCACGCGCTCCATCACCTGCGAGGTGGTCAGGCCTGAGCGCATCTTCAGGTGCAGCAGTTTTTGCATCAGTTCGGGGTTg                        >  1:184723/1‑141 (MQ=255)
                                                                                                                                  gcTGGCGCAAGCACCTCAAGCGCCTGCGTCCAGCGCCCGTCGATGATCGCCGCATACACCACGCGCTcc                                                                                             >  1:21063/1‑69 (MQ=12)
                                                                                                                                  gcTGGCGCAAGCACCTCAAGCGCCTGCG‑CCAGCGCCCGTCGATGATCGCCGCATACACCACGCGCTcc                                                                                             <  2:21063/68‑1 (MQ=17)
                                                                                                                                                  gAGGTGCTTGCG‑CCAGCGCCCGTCGATGATCGCCGCATACACCACGCGCTCCATCACCTGCg                                                                                   >  1:88066/1‑62 (MQ=255)
                                                                                                                                                  gAGGTGCTTGCG‑CCAGCGCCCGTCGATGATCGCCGCATACACCACGCGCTCCATCACCTGCg                                                                                   <  2:88066/62‑1 (MQ=255)
                                                                                                                                                  gAGGTGCTTGCG‑CCAGCGCCCGTCGATGATCGCCGCATACACCACGCGCTCCATCACCTGCGAGGTGGTCAGGCCTGAGCGCATCTTCAGGTGCAGCAGTTTTTGCATCAGTTCGGGGTTGGCCAGCATGTAGCCGACCCGCa  >  2:193316/1‑143 (MQ=255)
                                                                                                                                                  gAGGTGCTTGCG‑CCAGCGCCCGTCGATGATCGCCGCATACACCACGCGCTCCATCACCTGCGAGGTGGTCAGGCCTGAGCGCATCTTCAGGTGCAGCAGTTTTTGCATCAGTTCCGGGTTGGCCAGCATGTAGCCGACCCGCa  <  1:193316/143‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                               
AGCAACGCGGCACTGTCGGGAATCGCCGGATGGCGGGTCCAGATGTACATGCCTTCATCCGATTCGGTGAACAATTCAAAGCCCAGTCGATGCAGATGCCGGCCAACTTCCTGATGCGCCTCGGCCAGGCGCTGGCGCAGGCGCTTGAGGTGCTTGCG‑CCAGCGCCCGTCGATGATCGCCGCATACACCACGCGCTCCATCACCTGCGAGGTGGTCAGGCCTGAGCGCATCTTCAGGTGCAGCAGTTTTTGCATCAGTTCGGGGTTGGCCAGCATGTAGCCGACCCGCA  >  NC_002947/2887413‑2887701

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑