Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,887,566 T→C 8.5% K368R (AAG→AGG)  PP_2542 ← GntR family transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,887,5660TC 8.5% 132.4 / 3.1 47K368R (AAG→AGG) PP_2542GntR family transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base T (20/23);  new base C (2/2);  total (22/25)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.26e-01

CGGCACTGTCGGGAATCGCCGGATGGCGGGTCCAGATGTACATGCCTTCATCCGATTCGGTGAACAATTCAAAGCCCAGTCGATGCAGATGCCGGCCAACTTCCTGATGCGCCTCGGCCAGGCGCTGGCGCAGGCGCTTGAGGTGCTTGCG‑CCAGCGCCCGTCGATGATCGCCGCATACACCACGCGCTCCATCACCTGCGAGGTGGTCAGGCCTGAGCGCATCTTCAGGTGCAGCAGTTTTTGCATCAGTTCGGGGTTGGCCAGCATGTAGCCGACCCGCACGTTGGGCGAAAT  >  NC_002947/2887420‑2887714
                                                                                                                                                  |                                                                                                                                                     
cgGCACTGTCGGGAATCGCCGGATGGCGGGTCCAGATGTACATGCCTTCATCCGATTCGGTGAACAATTCAAAGCCCAGTCGATGCAGATGCCGGCCAACTTCCTGATGCGCCTCGGCCAGGCGCTGGCGCAGGCGCTTGAGGTGCTTg                                                                                                                                                     >  4:188576/1‑149 (MQ=255)
     cTGTCGGGAATCGCCGGATGGCGGGTCCAGATGTACATGCCTTCATCCGATTCGGTGAACAATTCAAAGCCCAGTCGATGCAGATGCCGGCCAACTTCCTGATGCGCCTCGGCCAGGCGCTGGCGCAGGCGCTTGAGGTGCTTGCG‑CCa                                                                                                                                               <  8:117149/149‑1 (MQ=255)
        tCGGGAATCGCCGGATGGCGGGTCCAGATGTACATGCCTTCATCCGATTCGGTGAACAATTCAAAGCCCAGTCGATGCAGATGCCGGCCAACTTCCTGATGCGCCTCGGCCAGGCGCTGGCGCAGGCGCTTGAGGTGCTTGCG‑CCAgcg                                                                                                                                            <  6:82741/149‑1 (MQ=255)
           ggAATCGCCGGATGGCGGGTCCAGATGTACATGCCTTCATCCGATTCGGTGAACAATTCAAAGCCCAGTCGATGCAGATGCCGGCCAACTTCCTGATGCGCCTCGGCCAGGCGCTGGCGCAGGCGCTTGAGGTGCTTGCG‑CCAGCGccc                                                                                                                                         <  5:179184/149‑1 (MQ=255)
            gAATCGCCGGATGGCGGGTCCAGATGTACATGCCTTCATCCGATTCGGTGAACAATTCAAAGCCCAGTCGATGCAGATGCCGGCCAACTTCCTGATGCGCCTCGGCCAGGCGCTGGCGCAAGCACCTCAAGCGCCTGCG‑CCAgc                                                                                                                                             <  1:116575/144‑1 (MQ=38)
            gAATCGCCGGATGGCGGGTCCAGATGTACATGCCTTCATCCGATTCGGTGAACAATTCAAAGCCCAGTCGATGCAGATGCCGGCCAACTTCCTGATGCGCCTCGGCCAGGCGCTGGCGCAAGCACCTCAAGCGCCTGCG‑CCAgc                                                                                                                                             >  2:116575/1‑144 (MQ=38)
             aaTCGCCGGATGGCGGGTCCAGATGTACATGCCTTCATCCGATTCGGTGAACAATTCAAAGCCCAGTCGATGCAGATGCCGGCCAACTTCCTGATGCGCCTCGGCCAGGCGCTGGTGCAGGCGCTTGAGGTGCTTGCG‑CCAGCGCCCGt                                                                                                                                       <  1:304109/149‑1 (MQ=255)
             aaTCGCCGGATGGCGGGTCCAGATGTACATGCCTTCATCCGATTCGGTGAACAATTCAAAGCCCAGTCGATGCAGATGCCGGCCAACTTCCTGATGCGCCTCGGCCAGGCGCTGGCGCAGGCGCTTGAGGTGCTTGCG‑CCAGCGCCCGt                                                                                                                                       <  3:188576/149‑1 (MQ=255)
                     gATGGCGGGTCCCGTTGTACATGCCTTCATCCGCTGCGGTGAACATTTCAAAGCCCAGGCGATGCTGATGCCGGCCAACTTCCTGATGCGCCTCGGCCTGGCGCTGGCGCTGGCGCTTGAGGTGCTTGCG‑CCAGCGCCCGTCGATGATc                                                                                                                               <  5:329006/149‑1 (MQ=255)
                       tGGCGGGTCCAGATGTACATGCCTTCATCCGATTCGGTGAACAATTCAAAGCCCAGTCGATGCAGATGCCGGCCAACTTCCTGATGCGCCTCGGCCAGGCGCTGGCGCAGGCGCTTGAGGTGCTTGCG‑CCAGCGCCCGTCGATGATcgc                                                                                                                             >  6:133389/1‑149 (MQ=255)
                                cAGATGTACTTGCCTTCATCCGATTCGGTGAACAATTCAAAGCCCAGTCGATGCAGATGCCGGCCAACTTCCTGATGCGCCTCGGCCAGGCGCTGGCGCAGGCGCTTGAGGTGCTTGCG‑CCAGCGCCCGTCGATGATCGCCGCATacac                                                                                                                    <  5:161400/149‑1 (MQ=255)
                                 aGATGTACATGCCTTCATCCGATTCGGTGAACAATTCAAAGCCCAGTCGATGCAGATGCCGGCCAACTTCCTGATGCGCCTCGGCCAGGCGCTGGCGCCGGCGCTTGAGGTGCTTGCG‑CCAGCGCCCGTCGATGCTCGCCGCATacac                                                                                                                    >  2:314999/1‑148 (MQ=255)
                                      tACATGCCTTCATCCGATTCGGTGAACAATTCAAAGCCCAGTCGATGCAGATGCCGGCCAACTTCCTGATGCGCCTCGGCCAGGCGCTGGCGCAGGCGCTTGAGGTGCTTGCG‑CCAGCGCCCGTCGATGATCGCCGCATACACCAcgcg                                                                                                              >  1:122026/1‑149 (MQ=255)
                                       aCATGCCTTCATCCGATTCGGTGAACAATTCAAAGCCCAGTCGATGCAGATGCCGGCCAACTTCCTGATGCGCCTCGGCCAGGCGCTGGCGCAGGCGCTTGAGGTGCTTGCG‑CCAGCGCCCGTCGATGATCGCCGCATACACCACgcgc                                                                                                             >  6:313619/1‑149 (MQ=255)
                                         aTGCCTTCATCCGATTCGGTGAACAATTCAAAGCCCAGTCGATGCAGATGCCGGCCAACTTCCTGATGCGCCTCGGCCAGGCGCTGGCGCAGGCGCTTGAGGTGCTTGCG‑CCAGCGCCCGTCGATGATCGCCGCATACACCACGCGCTc                                                                                                           <  5:313619/149‑1 (MQ=255)
                                         aTGCCTTCATCCGATTCGGTGAACAATTCAAAGCCCAGTCGATGCAGATGCCGGCCAACTTCCTGATGCGCCTCGGCCAGGCGCTGGCGCAGGCGCTTGAGGTGCTTGCG‑CCAGCGCCCGTCGATGATAGCCGCATACACCACGCGCTc                                                                                                           >  5:204804/1‑149 (MQ=255)
                                           gCCTTCATCCGATTCGGTGAACAATTCAAAGCCCAGTCGATGCAGATGCCGGCCAACTTCCTGATGCGCCTCGGCCAGGCGCTGGCGCAGGCGCTTGAGGTGCTTGCG‑CCAGCGCCCGTCGATGATCGCCGCATACACCACGCGCTCCa                                                                                                         >  3:240258/1‑149 (MQ=255)
                                                   ccGATTCGGTGAACAATTCAAAGCCCAGTCGATGCAGATGCCGGCAAACTTCCTGATGCGCCTCGGCCAGGCGCTGGCGCAGGCGCTTGAGGTGCTTGCG‑CCAGCGCCCGTCGATGATCCACGCATACACCACGCTCTACATCACCTGc                                                                                                 >  8:195231/1‑149 (MQ=255)
                                                    gttttCGGTTAACATTTCAACGCCCAGTCGTTGCAGATGCCGGCCAACTTCCTGATGCGCCTCGGCCAGGCGCTGGCGCAGGCGGTTGAGGTGCTTGCG‑CCAGCGCCCGTCGATGATCGCCGCATACACCACGCGCTCCATCACCTGc                                                                                                 <  7:195231/145‑1 (MQ=255)
                                                     gATTCGGTGAACAATTCAAAGCCCAGTCGATGCAGATGCCGGCCAACTTCCTGATGCGCCTCGGCCAGGCGCTGGCGCAGGCGCTTGAGGTGCTTGCG‑CCAGCGCCCGTCGATGATCGCCGCATACACCACGCGCTCCATCACCTGc                                                                                                 >  4:220775/1‑147 (MQ=255)
                                                                 atttCAACGCCCCGTGGATGCTGATGCCGGCCAACTTCCTGATGCGCGTGGGCCTGGGGCTGGGGCCGGGGGTTTAGGTGCTGGCG‑CCTGCGCCCGTCGATGATCGCCGCATACACCACGCGCTCCATCACCTGCGAGGTGGTCAGGc                                                                                    <  8:92227/146‑1 (MQ=255)
                                                                  aTTCAAAGCCCAGTCGATGCAGATGCCGGCCAACTTCCTGATGCGCCTCGGCCAGGCGCTGGCGCAGGCGCTTGAGGTGCTTGCG‑CCAGCGCCCGTCGATGATCGCCGCATACACCACGCGCTCCATCACCTGCGAGGTGGTCAGGc                                                                                    >  7:92227/1‑147 (MQ=255)
                                                                  aTTCAAAGCCCAGTCGATGCAGATGCCGGCCAACTTCCTGATGCGCCTCGGCCAGGCGCTGGCGCAGGCGCTTGAGGTGCTTGCG‑CCAGCGCCCGTCGATGATCGCCGCATACACCACGCGCTCCATCACCTGCGAGGTGGTCAGGCCt                                                                                  >  7:138687/1‑149 (MQ=255)
                                                                      aaaGCCCAGTCGATGCAGATGCCGGCCAACTTCCTGATGCGCCTCGGCCAGGCGCTGGCGCAGGCGCTTGAGGTGCTTGCG‑CCAGCGCCCGTCGATGATCGCCGCATACACCACGCGCTCCATCACCTGCGAGGTGGTCAGGCCTGAg                                                                               <  3:220775/148‑1 (MQ=255)
                                                                          cccAGTCGATGCAGATGCCGGCCAACTTCCTGATGCGCCTCGGCCAGGCGCTGGCGCAGGCGCTTGAGGTGCTTgcg‑c                                                                                                                                                 >  2:191372/1‑78 (MQ=255)
                                                                          cccAGTCGATGCAGATGCCGGCCAACTTCCTGATGCGCCTCGGCCAGGCCCTGGCGCAGGCGCTTGAGGTGCTTgcg‑c                                                                                                                                                 <  1:191372/78‑1 (MQ=255)
                                                                            cAGTCGATGCAGATGCCGGCCAACTTCCTGATGCGCCTCGGCCAGGCGCTGGCGCAGGCGCTTGAGGTGCTTGCG‑CCAGCGCCCGTCGATGATCGCCGCATACACCACGCGCTCCATCACCTGCGAGGTGGTCAGGCCTGAGCGCATCt                                                                        >  3:24954/1‑149 (MQ=255)
                                                                               tctaTGCATAGGCCGGCAAATTTCCTGATGCGCCTCGGCCAGGCGGTGGCGCTGGCGGTTGAGGTGCTTGCG‑CCAGCGCCCGTCGATGATCGCCGCATACACCACGCGCTCCATCACCTGCGAGGTGGTCAGGCCTGAGCGCATCTTc                                                                      <  8:138687/145‑1 (MQ=255)
                                                                                   tGCAGATGCCGGCCAACTTCCTGATGCGCCTCGGCCAGGCGCTGGCGCAGGCGCTTGAGGTGCTTGCG‑CCAGCGCCCGTCGATGATCGCCGCATACACCACGCGCTCCATCACCTGCGAGGTGGTCAGGCCTGAGCGCATCTTCAg                                                                    <  4:24954/146‑1 (MQ=255)
                                                                                             ggCCAACTTCCTGATGCGCCTCGGCCAGGCGCTGGCGCAGGCGCTTGAGGTGCTTGCG‑CCAGCGCCCGTCGATGATCGCCGCATACACCACGCGCTCCATCACCTGCGAGGTGGTCAGGCCTGAGCGCATCTTCAGGTGCAGCAGtttt                                                       >  1:58906/1‑149 (MQ=255)
                                                                                              gCCATCTTCCTGATGCGCCTGGGCCAGGCGCTGGCTCAGGCGCTTGAGGTGCTTGCG‑CAAGCGCCCGTCGATGATCGCCGCATACACCACGCGCTCCATCACCTGCGAGGTGGTCAGGCCTGAGCGCATCTTCAGGTGCAGCAGttttt                                                      <  4:240258/149‑1 (MQ=255)
                                                                                               ccAACTTCCTGATGCGCCTCGGCCAGGCGCTGGCGCAGGCGCTTGAGGTGCTTGCG‑CCAGCGCCCGTCGATGATCGCCGCATACACCACGCGCTCCATCACCTGCGAg                                                                                              <  7:150144/108‑1 (MQ=255)
                                                                                               ccAACTTCCTGATGCGCCTCGGCCAGGCGCTGGCGCAGGCGCTTGAGGTGCTTGCG‑CCAGCGCCCGTCGATGATCGCCGCATACACCACGCGCTCCATCACCTGCGAg                                                                                              >  8:150144/1‑108 (MQ=255)
                                                                                               caaaCTTCCTGATGCGCCTCGGCCAGGCGCTGGCGCGGGCGCTTGCGGTGCTTGCG‑CCAGCGCCCGTCGATGATCGCCGCATACACCACGCGCTCCATCACCTGCGAGGTGGTCAGGCCTGAGCGCATCTTCAGGTGCAGCAGTTTTTg                                                     <  3:276159/147‑1 (MQ=255)
                                                                                                   cTTCCTGATGCGCCTCGGCCAGGCGCTGGCGCAGGCGCTTGAGGTGCTTGCG‑CCAGCGCCCGTCGATGATCGCCGCATACACCACGCGCTCCATCACCTGCGAGGTGGTCAGGCCTGAGCGCATCTTCAGGTGCAGCAGTTTTTGCATc                                                 <  5:133389/149‑1 (MQ=255)
                                                                                                              gcCTCGGCCAGGCGCTGGCGCAGGCGCTTGAGGTGCTTGCG‑CCAGCGCCCGTCGATGATCGCCGCATACACCACGCGCTCCATCACCTGCGAGGTGGTCAGGCCTGAGCGCATCTTCAGGTGCAGCAGTTTTTGCATCAGTTCGGGGtt                                      <  2:58906/149‑1 (MQ=255)
                                                                                                                cTCGGCCAGGCGCTGGCGCACGCGCTTGACGTGCTTGCG‑CAAGCGCCCGTCGATGATCGCCGCATACAACACGCGCTCCATAACCTCCGAGGTTGACACGCCTGACCGCATCTTCAGGTGCAAAAGTTTTTGCATAAGTTAGGCGTTgt                                    >  2:300168/1‑148 (MQ=255)
                                                                                                                 tCGGCCAGGCGCTCGCGCAGGCGCTTGAGGTGCTTGCG‑CCAGCGCCCGTCGATGATCGCCGCATACACCACGCGCTCCATCACCTGCGAGGTGGTCAGGCCGGAGCGCATCTTCAGGTGCAGCAGTTTTTGCATCAGTTCGGGGTTGGc                                   >  7:123450/1‑149 (MQ=255)
                                                                                                                       cggCGCGGGCGCTGGCGCTTGTGGTGCTTGCG‑CCAGCGCCCGTCGATGATCGCCGCATACACCACGCGCTCCATCACCTGCGAGGTGGTCAGGCCTGAGCGCATCTTCAGGTGCAGCAGTTTTTGCATCAGTTCGGGGTTg                                     <  2:184723/140‑1 (MQ=255)
                                                                                                                       aGGCGCTGGCGCAGGCGCTTGAGGTGCTTGCG‑CCAGCGCCCGTCGATGATCGCCGCATACACCACGCGCTCCATCACCTGCGAGGTGGTCAGGCCTGAGCGCATCTTCAGGTGCAGCAGTTTTTGCATCAGTTCGGGGTTg                                     >  1:184723/1‑141 (MQ=255)
                                                                                                                           gcTGGCGCAAGCACCTCAAGCGCCTGCGTCCAGCGCCCGTCGATGATCGCCGCATACACCACGCGCTcc                                                                                                          >  1:21063/1‑69 (MQ=12)
                                                                                                                           gcTGGCGCAAGCACCTCAAGCGCCTGCG‑CCAGCGCCCGTCGATGATCGCCGCATACACCACGCGCTcc                                                                                                          <  2:21063/68‑1 (MQ=17)
                                                                                                                                           gAGGTGCTTGCG‑CCAGCGCCCGTCGATGATCGCCGCATACACCACGCGCTCCATCACCTGCg                                                                                                <  2:88066/62‑1 (MQ=255)
                                                                                                                                           gAGGTGCTTGCG‑CCAGCGCCCGTCGATGATCGCCGCATACACCACGCGCTCCATCACCTGCg                                                                                                >  1:88066/1‑62 (MQ=255)
                                                                                                                                           gAGGTGCTTGCG‑CCAGCGCCCGTCGATGATCGCCGCATACACCACGCGCTCCATCACCTGCGAGGTGGTCAGGCCTGAGCGCATCTTCAGGTGCAGCAGTTTTTGCATCAGTTCGGGGTTGGCCAGCATGTAGCCGACCCGCa               >  2:193316/1‑143 (MQ=255)
                                                                                                                                           gAGGTGCTTGCG‑CCAGCGCCCGTCGATGATCGCCGCATACACCACGCGCTCCATCACCTGCGAGGTGGTCAGGCCTGAGCGCATCTTCAGGTGCAGCAGTTTTTGCATCAGTTCCGGGTTGGCCAGCATGTAGCCGACCCGCa               <  1:193316/143‑1 (MQ=255)
                                                                                                                                               tGCTTGCG‑CAAGCGCCCGTCGATGATCGCCGCATACACCACGCGCTCCATCACCTGCGAGGTGGTCAGGCCTGAGCGCATCTTCAGGTGCAGCAGTTTTTGCATCAGTTCGGGGTTGGCCAGCATGTAGCCGACCCGCACGTTGGGCGa     <  2:122026/149‑1 (MQ=255)
                                                                                                                                                  ttGCG‑CCAGCGCCCGTCGATGATCGCCGCATACACCACGCGCTCCATCACCTGCGAGGTGGTCAGGCCTGAGCGCATCTTCAGGTGCAGCAGTTTTTGCATCAGTTCGGGGTTGGCCAGCATGTAGCCGACCCGCACGTTGGGCGAAAt  >  1:5299/1‑149 (MQ=255)
                                                                                                                                                  |                                                                                                                                                     
CGGCACTGTCGGGAATCGCCGGATGGCGGGTCCAGATGTACATGCCTTCATCCGATTCGGTGAACAATTCAAAGCCCAGTCGATGCAGATGCCGGCCAACTTCCTGATGCGCCTCGGCCAGGCGCTGGCGCAGGCGCTTGAGGTGCTTGCG‑CCAGCGCCCGTCGATGATCGCCGCATACACCACGCGCTCCATCACCTGCGAGGTGGTCAGGCCTGAGCGCATCTTCAGGTGCAGCAGTTTTTGCATCAGTTCGGGGTTGGCCAGCATGTAGCCGACCCGCACGTTGGGCGAAAT  >  NC_002947/2887420‑2887714

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑