| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 2,921,460 | G→A | 100% | G3494R (GGG→AGG) | PP_2561 → | hemolysin‑type calcium‑binding bacteriocin |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 2,921,460 | 0 | G | A | 97.7% | 86.4 / ‑6.2 | 29 | G3494R (GGG→AGG) | PP_2561 | hemolysin‑type calcium‑binding bacteriocin |
| Reads supporting (aligned to +/- strand): ref base G (0/0); major base A (15/13); minor base C (0/1); total (15/14) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.83e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
ACGTTGCTGGGGGAGGCCGGTAACGACAACCTGAACGGGGGCGCTGGCGATGACAGCCTGGATGGCGGCAACGGCAATGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACC‑GACACCCTGATCGGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGGTGGTGGGTGGGGCCGGTAACGACACCATGATCGCCACCGATGGAAACGATGTGTTCCAGTTCGCTGCAGGCTTTGGCAACGACCTGATCAT > NC_002947/2921316‑2921599 | acGTTGCTGGGGGAGGCCGGTAACGACAACCTGAACGGGGGCGCTGGCGATGACAGCCTGGATGGCGGCAACGGCAATGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACC‑GACACCCTGATCagggg < 3:165098/149‑1 (MQ=255) gggAGGCCGGTAACGACAACCTGAACGGGGGCGCTGGCGATGACAGCCTGGATGGCGGCAACGGCAATGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGAGACGTGGGTACC‑GACACCCTGATCAGGGGGGCCGGCAAc > 4:134697/1‑149 (MQ=255) aCGACAACCTGAACGGGGGCGCTGGCGATGACAGCCTGGATGGCGGCAACGGCAATGACACCCTGCAGGGCGGCGATCGTAACGACACATTGTTCGGCGACGTGGGTACC‑GACACCCTGATCAGGGGGGCCGGCAACGACTTCCTCAAc < 5:214185/149‑1 (MQ=255) gACAACCTGAACGGGGGCGCTGGCGATGACAGCCTGGATGGCGGCAACGGCAATGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTCCC‑GACAACCTGATCAGGGGGGCCGGCAACGACTTCCTCAACgg > 6:26507/1‑149 (MQ=255) gACAACCTGAACGGGGGCGCTGGCGATGACAGCCTGGATGGCGGCAACGGCAATGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACC‑GACACCCTGATCAGGGGGGCCGGCAACGACTTCCTCAACgg > 7:251862/1‑149 (MQ=255) gAACGGGGGCGCTGGCGATGACAGCCTGGATGGCGGCAACGGCAATGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACC‑GACACCCTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAg > 2:196884/1‑149 (MQ=255) gcTGGCGATGACAGCCTGGATGGCGGCAACGGCAATGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACC‑GACACCCTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATAc < 8:228570/149‑1 (MQ=255) tGGCGATGACAGCCTGGATGGCGGCAACGGCAATGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACC‑GACACCCTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGAATCGATACgg > 1:54434/1‑149 (MQ=255) gACAGCCTGGATGGCGGCAACGGCAATGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACC‑GACACCCTGATCAGGGGGGCCGGCAACGACt < 2:332697/112‑1 (MQ=255) cAGCCTGGATGGCGGCAACGGCAATGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACC‑GACACCCTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATACggtggt > 6:249785/1‑144 (MQ=255) cAGCCTGGATGGCGGCAACGGCAATGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACC‑GACACCCTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATACggtggt < 5:249785/144‑1 (MQ=255) aGCCTGGATGGCGGCAACGGCAATGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGGCCCCCCTGATCagggtgtcg > 6:61570/1‑95 (MQ=255) aGCCTGGATGGCGGCAACGGCAATGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGGCCCCCCTGATCagggtgtcg < 5:61570/100‑6 (MQ=255) aGCCTGGATGGCGGCAACGGCAATGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACC‑GACACCCTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGGTggtgggtg < 8:251862/148‑1 (MQ=255) aGCCTGGATGGCGGCAACGGCAATGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACC‑GACACCCTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGGTGgtgggtgg > 6:243661/1‑149 (MQ=255) gcggcAACGGCAATGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACC‑GACACCCTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGGTGGTGGGTGGGGCCGGTaa < 1:196884/148‑1 (MQ=255) gcggcAACGGCAATGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACC‑GACACCCTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGGTGGTGGGTGGGGCCGGTAAc < 5:243661/149‑1 (MQ=255) caaTGACACCCTGCAGGGCGGCGATGGTAACGACACAGTGTTCGGCGACGTGGGTACC‑GACACCCTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGGTGGTGGGTGGGGCCGGTAACGACACCATGa > 3:292254/1‑149 (MQ=255) aaTGACTCCCTGCAGGGCGGCGATGGTAACTACACATTGTTCGGCGACGTGTGTACC‑GACACCCTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGGGCGGCAGGTAACGATACGGTGGTGGGTGGGGCCGGTAACGACACCATGAt < 5:26507/149‑1 (MQ=255) aaTGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACC‑GACACCCTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGGTGGTGGGTGGGGCCGGTAACGa > 4:138245/1‑140 (MQ=255) aaTGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACC‑GACACCCTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGGTGGTGGGTGGGGCCGGTAACGa < 3:138245/140‑1 (MQ=255) ccTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACC‑GACACCCTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGGTGGTGGGTGGGGCCGGTACCGACACCATGATCGCCAcc > 3:70279/1‑148 (MQ=255) ggcgAGGGTAACGACACTTTGTGCGCCCCCGTGTGTACC‑GACACCCTGCTCCGGGGGGCCGGCAACGACTTCCTCAACGGCGCGGCAGGAAACGATACGGTGGTGGGGGGGGCCGGTAACGACACCATGATCGCCACCGATGGAAACGa < 3:107445/149‑1 (MQ=255) gcgATGGTAACGACACATTGTTCGGCGACGTGGGTACC‑GACACCCTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGGTGGTGGGTGGGGCCGGTAACGACACCATGATCGCCACCGATGGAAACGAt > 2:141117/1‑149 (MQ=255) gcgATGGTAACGACACATTGTTCGGCGACGTGGGTACC‑GACACCCTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGGTGGTGGGTGGGGCCGGTAACGACACCATGATCGCCACCGATGGAAACGAt < 1:141117/149‑1 (MQ=255) tcGAGACATAGTTCGGGGACTTGGGTACC‑GACACCCTGATCAGGGGGGGCGGGTACGACTTCGTCTACGGTGGGGCAGGTAACGATACGGTGGTGGGTGGGGCCGGTAACGACACCATGATCGCCACCGATGGAAACGATGTGTTCCa < 2:54434/147‑1 (MQ=255) aCGACACATTGTTCGGCGACGTGGGTACC‑GACACCCTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGGTGGTGGGTGGGGCCGGTAACGCCACCATGATCGCCACCGATGGAAACGATGTCTTCCAg > 6:230973/1‑149 (MQ=255) caTTGTTCGGCGACGTGGGTACC‑GACACCCTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGGTGGTGGGTGGGGCCGGTAACGACACCATGATCGCCACCGATGGAAACGATGTGTTCCAGTTCGCt > 3:159626/1‑149 (MQ=255) gACACCCTGATCAGGGTGGCCGTCAACGACTTCCTCAACGGTGCGGCATGTATCGATACGGTGGTTGGTGGGGCCGGTAACGACACCATGATCGCAACCGATGGAAACGATGTGTTCCAGTTCGCTGCAGGCTTTGGCAACGACCTGat < 5:230973/149‑1 (MQ=255) acCCTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGGTGGTGGGTGGGGACGGTAACGACACCATGATCGCCACCGATGGAAACGATGTGTTCCAGTTCGCTGCAGGCTTTGGCAACGACCTGatcat > 6:279900/1‑149 (MQ=255) ccTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGGTGGTGGGTGGGGCCGGTAACGACACCATGATCGCCACCGATGGAAACGATGTGTTCCAGTTCGCTGCAGGCTTTg > 2:297582/1‑131 (MQ=255) ccTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGGTGGTGGGTGGGGCCGGTAACGACACCATGATCGCCACCGATGGAAACGATGTGTTCCAGTTCGCTGCAGGCTTTg < 1:297582/131‑1 (MQ=255) | ACGTTGCTGGGGGAGGCCGGTAACGACAACCTGAACGGGGGCGCTGGCGATGACAGCCTGGATGGCGGCAACGGCAATGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACC‑GACACCCTGATCGGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGGTGGTGGGTGGGGCCGGTAACGACACCATGATCGCCACCGATGGAAACGATGTGTTCCAGTTCGCTGCAGGCTTTGGCAACGACCTGATCAT > NC_002947/2921316‑2921599 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |