Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,922,767 A→G 7.7% M829T (ATG→ACG) ‡ PP_2563 ← antibiotic biosynthesis protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,922,7670AG 7.7% 185.1 / 4.9 65M829T (ATG→ACG) ‡PP_2563antibiotic biosynthesis protein
Reads supporting (aligned to +/- strand):  ref base A (30/30);  new base G (3/2);  total (33/32)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.77e-01

GAACAGCGGGTGCCTGAAAACATGCTCCGCTACCGACTCGAACTGGAAATGCGTCAGGTAGATATACAAGGTTGACGAAGCGATCACCGCACCTGCCCGTGCAACCAGGGCGGGTACTGGCACCGA‑GCGGAACCAGACAAGTGCCAGCATCGCTGGCAGGGCGATGTCGACATAGTGTGTAAACGCTTGCCAGCCAACACCGGCAAGCGCCATCAGGTCCAACTCGCCAACCACCAGCACGGCCACTGCACTGGCCACCCATTTGCGTGAGATCGTGTCGGCATGGTG  >  NC_002947/2922619‑2922906
                                                                                                                                                     |                                                                                                                                           
gAACAGCGGGTGCCTGAAAACATGCTCCGCTACCGACTCGAACTGGAAATGCGTCAGGTAGATATACAAGGTTGACGAAGCGATCACCGCACCTGCCCGTGCAACCAGGGCGGGTACTGGCACCGA‑GCGGAACCAGACAAGTGCCAGCa                                                                                                                                             >  4:53363/1‑149 (MQ=255)
    aGCGGGTGCCTGAAAACATGCTCCGCTACCGACTCGAACTGGAAATGCGTCAGGTAGATATACAAGGTTGACGAAGCGATCACCGCACCTGCCCGTGCAACCAGGGCGGGTACTGGCACCGA‑GCGGAACCAGACAAGTGCCAGCATCGc                                                                                                                                         <  3:20726/149‑1 (MQ=255)
     gCGGGTGCCTGAAAACATGCTCCGCTACCGACTCGAACTGGAAATGCGTCAGGTAGATATACAAGGTTGACGAAGCGATCACCGCACCTGCCCGTGCAACCAGGGCGGGTACTGGCACCGA‑GCGGAACCAGACAAGTGCCAGCATCGCt                                                                                                                                        <  6:104332/149‑1 (MQ=255)
          tGCCTGAAAACATGCTCCGCTACCGACTCGAACTGGAAATGCGTCAGGTAGATATACAAGGTTGACGAAGCGATCACCGCACCTGCCCGTGCAACCAGGGCGGGTACTGGCACCGA‑GCGGAACCAGACAAGTGCCAGCGATGCTGGCac                                                                                                                                   <  6:248418/149‑2 (MQ=255)
             cTGAAAACAGGCTCCGCTACCGACTCGACCTGGAAATGCGTCAGGTAGATATACAAGGGTGACGAAGCGATCACCGCACCTGCCCGTGCAACCAGGGCGGGTACTGGCACCGA‑GCGGAACCAGACAAGTGCCAGCATCGCTGGCAGGGc                                                                                                                                <  5:38585/149‑1 (MQ=255)
               gAAAACATGCTCCGCTACCGACTCGAACTGGAAATGCGTCAGGTAGATATACAAGGTTGACGAAGCGATCACCGCACCTGCCCGTGCAACCAGGGCGGGTACTGGCACCGA‑GCGGAACCAGACAAGTGCCAGCATCGCTGGCAGGGCGa                                                                                                                              >  3:113432/1‑149 (MQ=255)
                      tGCTCCGCTACCGACTCGAACTGGAAATGCGTCAGGTAGATATACAAGGTTGACGAAGCGATCACCGCACCTGCCCGTGCAACCAGGGCGGGTACTGGCACCGAGGGGGAACCAGACAAGTGCCAGCAAAGCTGGCAGGGCGATGTCg                                                                                                                         >  1:114674/1‑148 (MQ=255)
                           cacTACCGACTCGAACTGGAAATGCGTCAGGTAGATATACAAGGTTGACGAAGCGATCACCGCACCTGCCCGTGCAACCAGGGCGGGTACTGGCACCGA‑GCGGAACCAGACAAGTGCCAGCATCGCTGGCAGGGCGATGTCGACATAgt                                                                                                                  <  6:313518/147‑1 (MQ=255)
                           cGCTACCGACTCGAACTGGAAATGCGTCAGGTAGATATACAAGGTTGACGAAGCGATCACCGCACCTGCCCGTGCAACCAGGGCGGGTACTGGCACCGA‑GCGGAACCAGACAAGTGCCAGCATCGCTGGCAGGGCGATGTCGACATAgt                                                                                                                  <  2:298574/149‑1 (MQ=255)
                                ccGACTCGAACTGGAAATGCGTCAGGTAGATATACAAGGTTGACGAAGCGATCACCGCACCTGCCCGTGCAACCAGGGCGGGTACTGGCACCGA‑GCGGAACCAGACAAGTGCCAGCATCGCTGGCAGGGCGATGTCGACATAGTGTGTa                                                                                                             <  4:113432/149‑1 (MQ=255)
                                       gAACTGGAAATGCGTCAGGTAGATATACAAGGTTGACGAAGCGATCACCGCACCTGCCCGTGCAACCAGGGCGGGTACTGGCACCGA‑GCGGAACCAGACAAGTGCCAGCATCGCTGGCAGGGCGATGTCGACATAGTGTGTAAACGCtt                                                                                                      >  3:291871/1‑149 (MQ=255)
                                           tGGAAATGCGTCAGGTAGATATACAAGGTTGACGAAGCGATCACCGCACCTGCCCGTGCAACCAGGGCGGGTACTGGCACCGA‑GCGGAACCAGACAAGTGCCAGCATCGCTGGCAGGGCGATGTCg                                                                                                                         >  1:125149/1‑126 (MQ=255)
                                           tGGAAATGCGTCAGGTAGATATACAAGGTTGACGAAGCGATCACCGCACCTGCCCGTGCAACCAGGGCGGGTACTGGCACCGA‑GCGGAACCAGACAAGTGCCAGCATCGCTGGCAGGGCGATGTCg                                                                                                                         <  2:125149/126‑1 (MQ=255)
                                             gAAATGCGTCAGGTAGATATACAAGGTTGACGAAGCGATCACCGCACCTGCCCGTGCAACCAGGGCGGGTACTGGCACCGA‑GCGGAACCAGACAAGTGCCAGCATCGCTGGCAGGGCGATGTCGACATAGTGTGTAAACGCTTgcca                                                                                                  <  1:67630/147‑1 (MQ=255)
                                               aaTGCGTCAGGTAGATATACAAGGTTGACGAAGCGATCACCGCACCTGCCCGTGCAACCAGGGCGGGTACTGGCACCGA‑GCGGAACCAGACAAGTGCCAGCATCGCTGGCAGGGCGATGTCGACATAGTGTGTAAACGCTTgccagcca                                                                                              <  3:17416/149‑1 (MQ=255)
                                                    gTCAGGTAGATATACAAGGTTGACGAAGCGATCACCGCACCTGCCCGTGCAACCAGGGCGGGTACTGGCACCGA‑GCGGAACCAGACAAGTGCCAGCATCGCTGGCAGGGCGATGTCGACATAGTGTGTAAACGCTTGCCAGCCAACAcc                                                                                         >  3:313346/1‑149 (MQ=255)
                                                            gATATACAAGGTTGACGAAGCGATCACCGCACCTGCCCGTGCAACCAGGGCGGGTACTGGCACCGA‑GCGGAACCAGACAAGTGCCAGCATCGCTGGCAGGGCGATGTCGACATAGTGTGTAAACGCTTGCCAGCCAACACCGGCAAgc                                                                                  <  3:242047/148‑1 (MQ=255)
                                                              tataCAAGGTTGACGAAGCGATCACCGCACCTGCCCGTGCAACCAGGGCGGGTACTGGCACCGA‑GCGGAACCAGACAAGTGCCAGCATCGCTGGCAGGGCGATGTCGACATAGTGTGTAAACGCTTGCCAGCCAACACCGGCAAgcgc                                                                                <  3:53363/148‑1 (MQ=255)
                                                              tataCAAGGTTGACGAAGCGATCACCGCACCTGCCCGTGCAACCAGGGCGGGTACTGGCACCGA‑GCGGAACCAGACAAGTGCCAGCATCGCTGGCAGGGCGATGTCGACATAGTGTGTAAACGCTTGCCAGCCAACACCGGCAAgcgc                                                                                >  6:229883/1‑148 (MQ=255)
                                                                  cAAGGTTGACGAAGCGATCACCTCACCTGCCCGTGCAACCAGGGCGGGTACTGGCACCGA‑GCGGAACCAGACAAGTGCCAGCATCGCTGGCAGGGCGATGTCGACATAGTGTGTAAACGCTTGCCAGCCAACACCGGCAAGCGCCATCa                                                                           <  3:199652/149‑1 (MQ=255)
                                                                  cAAGGTTGACGAAGCGATCACCGCACCTGCCCGTGCAACCAGGGCGGGTACTGGCACCGA‑GCGGAACCAGACAAGTGCCAGCATCGCTGGCAGGGCGATGTCGACATAGTGTGTAAACGCTTGCCAGCCAACACCGGCAAGCGCCATc                                                                            <  4:313346/148‑1 (MQ=255)
                                                                  cAAGGTTGACGAAGCGATCACCGCACCTGCCCGTGCAACCAGGGCGGGTACCGGCACCGA‑GCGGAACCAGACAAGTGCCAGCATCGCTGGCAGGGCGATGTCGACATAGTGTGTAAACGCTTGCCAGCCAACACCGGCAAGCGCCAt                                                                             >  7:233092/1‑147 (MQ=255)
                                                                     ggTTGACGAAGCGATCACCGCACCTGCCCGTGCAACCAGGGCGGGTACTGGCACCGA‑GCGGAACCAGACAAGTGCCAGCATCGCTGGCAGGGCGATGTCGACATAGTGTGTAAACGCTTGCCAGCCAACACCGGCAAGCGCCATCAg                                                                          <  6:155453/147‑1 (MQ=255)
                                                                     ggTTGACGAAGCGATCACCGCACCTGCCCGTGCAACCAGGGCGGGTACTGGCACCGA‑GCGGAACCAGACAAGTGCCAGCATCGCTGGCAGGGCGATGTCGACATAGTGTGTAAACGCTTGCCAGCCAACACCGGCAAGCGCCATCAg                                                                          >  5:155453/1‑147 (MQ=255)
                                                                      gTTGACGAAGCGATCACCGCACCTGCCCGTGCAACCAGGGCGGGTACTGGCACCGA‑GCGGAACCAGACAAGTGCCAGCATCGCTGGCAGGGCGATGTCGACATAGTGTGTAAACGCTTGCCAGCCAACACCGGCAAGCGCCATCAGGTc                                                                       >  7:92431/1‑149 (MQ=255)
                                                                            gAAGCGATCACCGCACCTGCCCGTGCAACCAGGGCGGGTACTGGCACCGA‑GCGGAACCAGACAAGTGCCAGCATCGCTGGCAGGGCGATGTCGACATAGTGTGTAAACGCTTGCCAGCCAACACCGGCAAGCGCCATCAGGTCCAACTc                                                                 >  3:13163/1‑149 (MQ=255)
                                                                            gAAGCGATCACCGCACCTGCCCGTGCAACCAGGGCGGGTACTGGCACCGA‑GCGGAACCAGACAAGTGCCAGCATCGCTGGCAGGGCGATGTCGACATAGTGTGTAAACGCTTGCCAGCCAACACCGGCAAGCGCCATCAGGTCCAACTc                                                                 >  7:71045/1‑149 (MQ=255)
                                                                            gAAGCGATCACCGCACCTGCCCCTGCAACCAGGGCGGGTACTGGCACCGA‑GCGGAACCAGACAAGTGCCAGCATCGCTGGCAGGCCGATGTCGAGATACTGAGAAAACGCTTGCCAGCCAACACAGGCAAGCGCCATCAGGTCCAACTc                                                                 >  7:205074/1‑149 (MQ=255)
                                                                                cGATCACCGCACCTGCCCGTGCAACCAGGGCGGGTACTGGCACCGA‑GCGGAACCAGACAAGTGCCAGCATCGCTGGCAGGGCGATGTCGACATAGTGTGTAAACGCTTGCCAGCCAACACCGGCAAGCGCCATCAGGTCCAACTCGcc                                                              >  7:44534/1‑148 (MQ=255)
                                                                                  aTCACCGCACCTGCCCGTGCAACCAGGGCGGGTACTGGCACCGA‑GCGGAACCAGACAAGTGCCAGCATCGCTGGCAGGGCGATGTCGACATAGTGTGTAAACGCTTGCCAGCCAACACCGGCAAGCGCCATCAGGTCCAACTCGCCAac                                                           >  5:256823/1‑149 (MQ=255)
                                                                                          aCCTGCCCGTGCAACCAGGGCGGGTACTGGCACCGA‑GCGGAACCAGACAAGTGCCAGCATCGCTGGCAGGGCGATGTCGACATAGTGTGTAAACGCTTGCCAGCCAACACCGGCAAGCGCCATCAGGTCCAACTCGCCAACCACCAGCa                                                   >  2:274906/1‑149 (MQ=255)
                                                                                           ccTGCCCGTGCAACCAGGGCGGGTACTGGCACCGA‑GCGGAACCAGACAAGTGCCAGCATCGCTGGCAGGGCGATGTCGACATAGTGTGTAAACGCTTGCCAGCCAACACCGGCAAGCGCCATCAGGTCCAACTCGCCAACCACCAGCAc                                                  >  2:198490/1‑149 (MQ=255)
                                                                                              gCCCGTGCAACCAGGGCGGGTACTGGCACCGA‑GCGGAACCAGACAAGTGCCAGCATCGCTGGCAGGGCGATGTCGACATAGTGTGTAAACGCTTGCCAGCCAACACCGGCAAGCGCCATCAGGTCCAACTCg                                                                <  7:56646/132‑1 (MQ=255)
                                                                                              gCCCGTGCAACCAGGGCGGGTACTGGCACCGA‑GCGGAACCAGACAAGTGCCAGCATCGCTGGCAGGGCGATGTCGACATAGTGTGTAAACGCTTGCCAGCCAACACCGGCAAGCGCCATCAGGTCCAACTCg                                                                >  8:56646/1‑132 (MQ=255)
                                                                                              gCCCGTGCAACCAGGGCGGGTACTGGCACCGA‑GCGGAACCAGACAAGTGCCAGCATCGCTGGCAGGGCGATGTCGACATAGTGTGTAAACGCTTGCCAGCCAACACCGGCAAGCGCCATCAGGTCCAACTCGCCAACCACCAGCACGGc                                               >  4:222144/1‑149 (MQ=255)
                                                                                              gCCCGTGCAACCAGGGCGGGTACTGGCACCGA‑GCGGAACCAGACAAGTGCCAGCATCGCTGGCAGGGCGATGTCGACATAGTGTGTAAACGCTTGCCAGCCAACACCGGCAAGCGCCATCAGGTCCAACTCGCCAACCACCAGCACGGc                                               >  6:202933/1‑149 (MQ=255)
                                                                                               cccGTGCAACCAGGGCGGGTACTGGCACCGA‑GCGGAACCAGACAAGTGCCAGCATCGCTGGCAGGGCGATGTCGACATAGTGTGTAAACGCTTGCCAGCCAACACCGGCAAGCGCCATCAGGTCCAACTCGCCAACCACCAGCACGGc                                               >  2:322103/1‑148 (MQ=255)
                                                                                               cccGTGCAACCAGGGCGGGTACTGGCACCGA‑GCGGAACCAGACAAGTGCCAGCATCGCTGGCAGGGCGATGTCGACATAGTGTGTAAACGCTTGCCAGCCAACACCGGCAAGCGCCATCAGGTCCAACTCGCCAACCACCAGCACGGc                                               <  6:256823/148‑1 (MQ=255)
                                                                                                ccGTGCAACCAGGGCGGGTACCGGCACCGA‑GCGGAACCAGACAAGTGCCAGCATCGCTGGCAGGGCGATGTCGACATAGTGTGTAAACGCTTGCCAGCCAACACCGGCAAGCGCCATCAGGTCCAACTCGCCAACCACCAGCACGGCca                                             <  8:233092/149‑1 (MQ=255)
                                                                                                  gTGCAACCAGGGCGGGTACTGGCACCGA‑GCGGAACCAGACAAGTGCCAGCATCGCTGGCAGGGCGATGTCGACATAGTGTGTAAACGCTTGCCAGCCAACACCGGCAAGCGCCATCAGGTCCAACTCGCCAACCACCAGCACGGCcact                                           <  4:291871/149‑1 (MQ=255)
                                                                                                   tGCAACCAGGGCGGGTACTGGCACCGA‑GCGGAACCAGACAAGTGCCAGCATCGCTGGCAGGGCGATGTCGACATAGTGTGTAAACGCTTGCCAGCCAACACCGGCAAGCGCCATCAGGTCCAACTCGCCAACCACCAGCACGGCcactg                                          <  1:198490/149‑1 (MQ=255)
                                                                                                   tGCAACCAGGGCGGGTACTGGCACCGA‑GCGGAACCAGACAAGTGCCAGCATCGCTGGCAGGGCGATGTCGACATAGTGTGTAAACGCTTGCCAGCCAACACCGGCAAGCGCCATCAGGTCCAACTCGCCAACCACCAGCACGGCcactg                                          <  2:114674/149‑1 (MQ=255)
                                                                                                     cAACCAGGGCGGGTACTGGCACCGA‑GCGGAACCAGACAAGTGCCAGCATCGCTGGCAGGGCGATGTCGACATAGTGTGTAAACGCTTGCCAGCCAACACCg                                                                                        <  2:135210/101‑1 (MQ=255)
                                                                                                     cAACCAGGGCGGGTACTGGCACCGA‑GCGGAACCAGACAAGTGCCAGCATCGCTGGCAGGGCGATGTCGACATAGTGTGTAAACGCTTGCCAGCCAACACCg                                                                                        >  1:135210/1‑101 (MQ=255)
                                                                                                      aaCCAGGGCGGGTACTGGCACCGA‑GCGGAACCAGACAAGTGCCAGCATCGCTGGCAGGGCGATGTCGACATAGTGTGTAAACGCTTGCCAGCCAACACCGGCAAGCGCCATCAGGTCCAACTCGCCAACCACCAGCACGGc                                               <  8:130960/141‑1 (MQ=255)
                                                                                                      aaCCAGGGCGGGTACTGGCACCGA‑GCGGAACCAGACAAGTGCCAGCATCGCTGGCAGGGCGATGTCGACATAGTGTGTAAACGCTTGCCAGCCAACACCGGCAAGCGCCATCAGGTCCAACTCGCCAACCACCAGCACGGCcagatcgg                                       >  7:130960/1‑143 (MQ=255)
                                                                                                        ccAGGGCGGGTACTGGCACCGA‑GCGGAACCAGACAAGTGCCAGCATCGCTGGCAGGGCGATGTCGACATAGTGTGTAAACGCTTGCCAGCCAACACCGGCAAGCGCCATCAGGTCCAACTCGCCAACCACCAGCACGGCcactgcactg                                     <  8:44534/149‑1 (MQ=255)
                                                                                                                     tGGCACCGA‑GCGGAACCAGACAAGTGCCAGCATCGCTGGCAGGGCGATGTCGACATAGTGTGTAAACGCTTGCCAGCCAACACCGGCAAGCGCCAGAAGGTCCAACTCGCCAACCACCAGCACGGCCACTGCACTCGCCACCCATTTGc                        >  8:144920/1‑149 (MQ=255)
                                                                                                                       gCACCGA‑GCGGAACCAGACAAGTGCCAGCATCGCTGGCAGGGCGATGTCGACATAGTGTGTAAACGCTTGCCAGCCAACACCGGCAAGCGCCATCAGGTCCAACTCGCCAACCACCAGCACGGCCACTGCACTGGCCACCCATTTGCGt                      <  4:13163/149‑1 (MQ=255)
                                                                                                                         aCCGA‑GCGGAACCAGACAAGTGCCAGCATCGCTGGCAGGGCGATGTCGACATAGTGTGTAAACGCTTGCCAGCCAACACCGGCAAGCGCCATCAGGTCCAACTCGCCAACCACCAGCACGGCCACTGCACTGGCCACCCATTTGCGt                      >  6:205239/1‑147 (MQ=255)
                                                                                                                                cGGAACCAGACAAGTGCCAGCATCGCTGGCAGGGCGATGTCGACATAGTGTGTAAACGCTTGCCAGCCAACACCGGCAAGCGCCATCAGGTCCAACTCGCCAACCACGAGCACGGCCACTGCACTGGCCACCCATTTGCGTGAGATCgt              >  8:226631/1‑149 (MQ=255)
                                                                                                                                cGGAACCAGACAAGTGCCAGCATCGCTGGCAGGGCGATGTCGACATAGTGTGTAAACGCTTGCCAGCCAACACCGGCAAGCGCCATCAGGTCCAACTCGCCAACCACCAGCACGGCCACTGCACTGGCCACCCATTTGCGTGAGATCgt              <  8:71045/149‑1 (MQ=255)
                                                                                                                                 ggAACCAGACAAGTGCCAGCATCGCTGGCAGGGCGATGTCGACATAGTGTGTAAACGCTTGCCAGCCAACACCGGCAAGCGCCATCAGGTCCAACTCGCCAACCACCAGCACGGCCACTGCACTGGCCACCCATTTGCGTGAGATCgtg             >  7:75134/1‑149 (MQ=255)
                                                                                                                                  gAACCAGACAAGTGCCAGCATCGCTGGCAGGGCGATGTCGACATAGTGTGTAAACGCTTGCCAGCCAACACCGGCAAGCGCCATCAGGTCCAACTCGCCAACCACCAGCACGGCCACTGCACTGGCCACCCATTTGCGTGAGATCgtgt            <  1:322103/149‑1 (MQ=255)
                                                                                                                                   aaCCAGACAAGTGCCAGCATCGCTGGCAGGGCGATGTCGACATAGTGTGTAAACGCTTGCCAGCCAACACCGGCAAGCGCCATCAGGTCCAACTCGCCAACCACCAGCACGGCCACTGCACTGGCCACCCATTTGCGTGAGATCGTGTc           <  1:274906/149‑1 (MQ=255)
                                                                                                                                    aCCAGACAAGTGCCAGCATCGCTGGCAGGGCGATGTCGACATAGTGTGTAAACGCTTGCCAGCCAACACCGGCAAGCGCCATCAg                                                                          <  7:268060/85‑1 (MQ=255)
                                                                                                                                    aCCAGACAAGTGCCAGCATCGCTGGCAGGGCGATGTCGACATAGTGTGTAAACGCTTGCCAGCCAACACCGGCAAGCGCCATCAg                                                                          >  8:268060/1‑85 (MQ=255)
                                                                                                                                    aCCAGACAAGTGCCAGCATCGCTGGCAGGGCGATGTCGACATAGTGTGTAAACGCTTGCCAGCCAACACCGGCAAGCGCCATCAGGTCCAACTCGCCAACCACCAGCACGGCCACTGCACTGGCCACCCATTTGCGTGAGATCGTGTCg          <  3:222144/149‑1 (MQ=255)
                                                                                                                                          cAAGTGCCAGCATCGCTGGCAGGGCGATGTCGACATAGTGTGTAAACGCTTGCCAGCCAACACCGGCAAGCGCCATCAGGTCCAACTCGCCAACCACCAGCACGGCCACTGCACTGGCCACCCATTTGCGTGAGATCGTGTCGGCATgg    <  8:92431/149‑1 (MQ=255)
                                                                                                                                           aaGTGCCAGCATCGCTGGCAGGGCGATGTCGACATAGTGTGTAAACGCTTGCCAGCCAACACCGGCAAGCGCCATCAGGTCCAACTCGCCAACCACCAGCACGGCCACTGCACTGGCCACCCATTTGCGTGAGATCGTGTCGGCATGGt   >  1:336446/1‑149 (MQ=255)
                                                                                                                                            cctGCCAGCGATGCTGGCAGGGCGATGTCGACATAGTGTGTAAACGCTTGCCAGCCAACACCGGCAAGCGCCATCAGGTCCAACTCGCCAACCACCAGCACGGCCACTGCACTGGCCACCCATTTGCGTga                    <  5:312003/129‑1 (MQ=255)
                                                                                                                                            cctGCCAGCGATGCTGGCAGGGCGATGTCGACATAGTGTGTAAACGCTTGCCAGCCAACACCGGCAAGCGCCATCAGGTCCAACTCGCCAACCACCAGCACGGCCACTGCACTGGCCACCCATTTGCGTga                    >  6:312003/3‑131 (MQ=255)
                                                                                                                                            cctGCCAGCGATGCTGGCAGGGCGATGTCGACATAGTGTGTAAACGCTTGCCAGCCAACACCGGCAAGCGCCATCAGGTCCAACTCGCCAACCACCAGCACGGCCACTGCACTGGCCACCCATTTGCGTGAGATCGTGTCGGCATGGTg  >  2:304843/3‑149 (MQ=255)
                                                                                                                                             gTGCCAGCATCGCTGGCAGGGCGATGTCGACATAGTGTGTAAACGCTTGCCAGCCAACACCGGCAAGCGCCATCAGGTCCAACTCGCCAACCACCAGCACGGCCACTGCACTGGCCACCCATTTGCGTGAGATCGTGTCGGCATGGTg  >  8:168376/1‑148 (MQ=255)
                                                                                                                                             gTGCCAGCATCGCTGGAAGGGCGATGTCGACATAGTGTGTAAACGCTTGACAGCCAACAACGGCAAGCGCCATCAGGTCCAACTCGCCAACCACAAGCACGGCCACTGAACTGGCCACCCATTTGCGTGAGATCGTGTCGGCaggttg  >  2:325873/1‑143 (MQ=255)
                                                                                                                                             ctGCCAGCGATGCTGGCAGGGCGATGTCGACATAGTGTGTAAACGCTTGCCAGCCAACACCGGCAAGCGCCATCAGGTCCAACTCGCCAACCACCAGCACGGCCACTGCACTGGCCACCCATTTGCGTGAGATCGTGTCGGCATGGTg  >  7:310288/2‑148 (MQ=255)
                                                                                                                                                     |                                                                                                                                           
GAACAGCGGGTGCCTGAAAACATGCTCCGCTACCGACTCGAACTGGAAATGCGTCAGGTAGATATACAAGGTTGACGAAGCGATCACCGCACCTGCCCGTGCAACCAGGGCGGGTACTGGCACCGA‑GCGGAACCAGACAAGTGCCAGCATCGCTGGCAGGGCGATGTCGACATAGTGTGTAAACGCTTGCCAGCCAACACCGGCAAGCGCCATCAGGTCCAACTCGCCAACCACCAGCACGGCCACTGCACTGGCCACCCATTTGCGTGAGATCGTGTCGGCATGGTG  >  NC_002947/2922619‑2922906

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑