Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 23,203 A→G 5.6% R10G (AGG→GGG)  PP_0018 → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_00294723,2030AG 5.6% 212.1 / 2.0 72R10G (AGG→GGG) PP_0018hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (32/35);  new base G (2/2);  total (34/38)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.41e-01

ACTCCATCCCTCACGGGGACCGCTTGTCAAATCGAAAGGCCCTGCTATACAGTTCTTGTCAAAGGATTTGTCAAATGATGTAGAATCCGGTCCCTCGTTGGGCGCGGTGGAGGAGTTAACATGTCAGAACGCGAAGGGGTCTTAGCAAGGGCGCCGCTTATCTATGCGTTGAGCGTAATCAGATTTGCCCCGATTCTTAAGCTGCCAAAGCTGATCCCAGACATTCAGCACACGATCCGTCAGAGCCTCCCGGGCTTCTTCCAGATGGTCAAGGGCGTGCCGCCTGGGGTGATG  >  NC_002947/23056‑23349
                                                                                                                                                   |                                                                                                                                                  
aCTCCATCCCTCACGGGGACCGCTTGTCAAATCGAAAGGCCCTGCTATACAGTTCTTGTCAAAGGATTTGTCAAATGATGTAGAATCCGGTCCCTCGTTGGGCGCGGTGGAGGAGTTAACATGTCAGAACGCGAAGGGGTCTTAGCAAg                                                                                                                                                   <  8:233299/149‑1 (MQ=255)
     aTCCCTCACGGGGACCGCTTGTCAAATCGAAAGGCCCTGCTATACAGTTCTTGTCAAAGGATTTGTCAAATGATGTAGAATCCGGTCCCTCGTTGGGCGCGGTGGAGGAGTTAACATGTCAGAACGCGAAGGGGTCTTAGCaa                                                                                                                                                    <  7:239492/143‑1 (MQ=255)
     aTCCCTCACGGGGACCGCTTGTCAAATCGAAAGGCCCTGCTATACAGTTCTTGTCAAAGGATTTGTCAAATGATGTAGAATCCGGTCCCTCGTTGGGCGCGGTGGAGGAGTTAACATGTCAGAACGCGAAGGGGTCTTAGCaa                                                                                                                                                    >  8:239492/1‑143 (MQ=255)
     aTCCCTCACGGGGACCGCTTGTCAAATCGAAAGGCCCTGCTATACAGTTCTTGTCAAAGGATTTGTCAAATGATGTAGAATCCGGTCCCTCGTTGGGCGCGGTGGAGGAGTTAACATGTCAGAACGCGAAGGGGTCTTAGCAAGGgcgc                                                                                                                                              <  8:80611/149‑1 (MQ=255)
     aTCCCTCACGGGGACAGCTAGTCAAATCGAAAGGCCCTGCTATACAGTTCTTGTCAAAGGATTTGTCAAATGATGTAGAATACGGTCCCTCGTTGGGCGCGGTGGAGGAGTTAACATCTCAGAACGCCAAGGTGTCTTACCAAGGgcgc                                                                                                                                              >  8:161496/1‑149 (MQ=255)
         cTCACGGGGACCGCTTGTCAAATCGAAAGGCCCTGCTATACACTTCTTGTCAAAGGATTTGTCAAATGATGTAGAATCCGGTCCCTCGTTGGGCGCGGTGGAGGAGTTAACATGTCAGAACGCGAAGGGGTCTTAGCAAGGGCGCCGCt                                                                                                                                          <  2:335288/149‑1 (MQ=255)
              ggggACCGCGTGTCAAATCGAAAGGCCCTGCTATACAGTTCTTGTCAAAGGATTTGTCAAATGATGTAGAATCCGGTCCCTCGTTGGGCGCGGGGGAGGAGTTAACATGTCAGAACTCGAAGGGGTCTTAGTAAGGTCGACGAGTATc                                                                                                                                      >  8:264591/1‑148 (MQ=255)
                ggACCGCTTGTCAAATCGAAAGGCCCTGCTATACAGTTCTTGTCAAAGGATTTGTCAAATGATGTAGAATCCGGTCACTCGTTGGGCGCGGTGGAGGAGTTAACATGTCAGAACGCGAAGGGGTCTTAGCAAGGGCGCCGCTTATCTAt                                                                                                                                   >  2:255402/1‑149 (MQ=255)
                 gACCGCTTGTCAAATCGAAAGGCCCTGCTATACAGTTCTTGTCAAAGGATTTGTCAAATGATGTAGAATCCGGTCCCTCGTTGGGCGCGGTGGAGGAGTTAACATGTCAGAACGCGAAGGGGTCTTAGCAAGGGCGCCGCTTATCTATg                                                                                                                                  <  7:50723/149‑1 (MQ=255)
                      cTTGTCAAATCGAAAGGCCCTGCTATACAGTTCTTGTCAAAGGATTTGTCAAATGATGTAGAATCCGGTCCCTCGTTGGGCGCGGTGGAGGAGTTAACATGTCAGAACGCGAAGGGGTCTTAGCAGGGGCGCCGCTTATCTATGCGtt                                                                                                                              >  8:167114/1‑148 (MQ=255)
                      cTTGTCAAATCGAAAGGCCCTGCTATACAGTTCTTGTCAAAGGATTTGTCAAATGATGTAGAATCCGGTCCCTCGTTGGGCGCGGTGGAGGAGTTAACATGTCAGAACGCGAAGGGGTCTTAGCAGGGGCGCCGCTTATCTATGCGtt                                                                                                                              >  8:162398/1‑148 (MQ=255)
                       ttGTCAAATCGAAAGGCCCTGCTATACAGTTCTTGTCAAAGGATTTGTCAAATGATGTAGAATCCGGTCCCTCGTTGGGCGCGGTGGAGGAGTTAACATGTCAGAACGCGAAGGGGTCTTAGCAAGGGCGCCGCTTATCTATGCGtt                                                                                                                              >  1:46112/1‑147 (MQ=255)
                          tCAAATCGAAAGGCCCTGCTATACAGTTCTTGTCAAAGGATTTGTCAAATGATGTAGAATCCGGTCCCTCGTTGGGCGCGGTGGAGGAGTTAACATGTCAGAACGCGAAGGGGTCTTAGCAAGGGCGCCGCTTATCTATGCGTTGAGc                                                                                                                          <  5:218647/148‑1 (MQ=255)
                            aaaTCGAAAGGCCCTGCTATACAGTTCTTGTCAAAGGATTTGTCAAATGATGTAGAATCCGGTCCCTCGTTGGGCGCGGTGGAGGAGTTAACATGTCAGAACGCGAAGGGGTCTTAGCAAGGGCGCCGCTTATCTATGCGTTTAGCGt                                                                                                                        <  4:112089/148‑1 (MQ=255)
                            aaaTCGAAAGGCCCTGCTATACAGTTCTTGTCAAAGGATTTGTCAAATGATGTAGAATCCGGTCCCTCGTTGGGCGCGGTGGAGGAGTTAACATGTCAGAACGCGAAGGGGTCTTAGCAAGGGCGCCGCTTATCTATGCGTTGAGCGt                                                                                                                        >  5:224401/1‑148 (MQ=255)
                             aaTCGAAAGGCCCTGCTATACAGTTCTTGTCAAAGGATTTGTCAAATGATGTAGAATCCGGTCCCTCGTTGGGCGCGGTGGAGGAGTTAACATGTCAGAACGCGAAGGGGTCTTAGCAAGGGCGCCGCTTATCTATGCGTTGAGCGTaa                                                                                                                      >  4:72619/1‑149 (MQ=255)
                                  aaaGGCCCTGCTATACAGTTCTTGTCAAAGGATTTGTCAAATGATGTAGAATCCGGTCCCTCGTTGGGCGCGGTGGAGGAGTTAACATGTCAGAACGCGAAGGGGTCTTAGCAAGGGCGCCGCTTATCTATGCGTTGAGCGTAAt                                                                                                                     >  5:144858/1‑145 (MQ=255)
                                  aaaGGCCCTGCTATACAGTTCTTGTCAAAGGATTTGTCAAATGATGTAGAATCCGGTCCCTCGTTGGGCGCGGTGGAGGAGTTAACATGTCAGAACGCGAAGGGGTCTTAGCAAGGGCGCCGCTTATCTATGCGTTGAGCGTAAt                                                                                                                     >  1:176704/1‑145 (MQ=255)
                                  aaaGGCCCTGCTATACAGTTCTTGTCAAAGGATTTGTCAAATGATGTAGAATCCGGTCCCTCGTTGGGCGCGGTGGAGGAGTTAACATGTCAGAACGCGAAGGGGTCTTAGCAAGGGCGCCGCTTATCTATGCGTTGAGCGTAAt                                                                                                                     >  7:142650/1‑145 (MQ=255)
                                       gccTTCTATACATTTTTTTTGAAATGTTTTGTCAATTGATGTAGAATCCGGTCCCTGGTTGGGCGGGGTGGAGGAGTTTACTTGTTAGTACGGGAAGGTGTCTTAGCATGGGCGGCGCTTATCTATGCGTTGAGCGTATTCAGATTTgg                                                                                                            <  2:303749/148‑2 (MQ=255)
                                         cTGCTATACAGTTCTTGTCAAAGGATTTGTCAAATGATGTAGAATCCGGTCCCTCGTTGGGCGCGGTGGAGGAGTTAACATGTCAGAACGCGAAGGGGTCTTAGCAAGGGCGCCGCTTATCTATGCGTTGAGCGTAATCAGATTTGccc                                                                                                          <  3:197008/149‑1 (MQ=255)
                                         cTGCTATACAGTTCTTGTCAAAGGATTTGTCAAATGATGTAGAATCCGGTCCCTCGTTGGGCGCGGTGGAGGAGTTAACATGTCAGAACGCGAAGGGGTCTTAGCAAGGGCGCCGCTTATCTATGCGTTGAGCGTAATCAGATTTGccc                                                                                                          >  4:144989/1‑149 (MQ=255)
                                          tGCTATACATTTCTTGTCAAAGGATTTGTCAAATGATGTATAATCCGGTCCCTCGTTGGGCGCGGTGGAGGAGTTAACATGTAAGAAAGCGAAGGGGTCTTAGCAAGGGCGCCGATTATATATGCGTTTAGCGTAATc                                                                                                                    >  8:117419/1‑138 (MQ=255)
                                          tGCTATACAGTTATTGTCAAAGGATTTTTCAAATGATGTAGAATCCGGTCCCTAGTTGGGCGCGGTGGATGAGTTAACATGTCAGAACGCGAAGGGGTCTTAGCAAGGGCGCCGCTTATCTATGCGTTGAGCGTAATc                                                                                                                    <  7:117419/138‑1 (MQ=255)
                                             tataCAGTTCTTGTCAAAGGATTTGTCAAATGATGTAGAATCCGGTCCCTCGTTGGGCGCGGTGGAGGAGTTAACATGTCAGAACGCGAAGGGGTCTTAGCAAGGGCGCCGCTTATCTATGCGTTGAGCGTAATCAGATTTGCCCCGAt                                                                                                      >  3:66829/1‑149 (MQ=255)
                                              ataCAGTTCTTGTCAAAGGATTTGTCAAATGATGTAGAATCCGGTCCCTCGTTGGGCGCGGTGGAGGAGTTAACATGTCATAACGCGAAGGGGTCTTAGCAAGGGCGCCGCTTATCTATGCGTTGAGCGTAATCAGATTTGCCCCGAtt                                                                                                     >  5:189014/1‑149 (MQ=255)
                                               taCAGTTCTTGTCAAAGGATTTGTCAAATGATGTAGAATCCGGTCCCTCGTTGGGCGCGGTGGAGGAGTTAACATGTCAGAACGCGAAGGGGTCTTAGCAAGGGCGCCGCTTATCTATGCGTTGAGCGTAATCAGATTTGCCCCGATTc                                                                                                    >  3:68487/1‑149 (MQ=255)
                                               taCAGTTCTTGTCAAAGGATTTGTCAAATGATGTAGAATCCGGTCCCTCGTTGGGCGCGGTGGAGGAGTTAACATGTCAGAACGCGAAGGGGTCTTAGCAAGGGCGCCGCTTATCTATGCGTTGAGCGTAATCAGATTTGCCCCGATTc                                                                                                    >  4:1812/1‑149 (MQ=255)
                                                  aGTTCTTGTCAAAGGATTTGTCAAATGATGTAGAATCCGGTCCCTCGTTGGGCGCGGTGGAGGAGTTAACATGTCAGAACGCGAAGGGGTCTTAGCAGGGGCGCCGCTTATCTATGCGTTGAGCGTAATCAGATTTGCCCCGATTCTTa                                                                                                 <  7:162398/149‑1 (MQ=255)
                                                  aGTTCTTGTCAAAGGATTTGTCAAATGATGTAGAATCCGGTCCCTCGTTGGGCGCGGTGGAGGAGTTAACATGTCAGAACGCGAAGGGGTCTTAGCAGGGGCGCCGCTTATCTATGCGTTGAGCGTAATCAGATTTGCCCCGATTCTTa                                                                                                 <  7:167114/149‑1 (MQ=255)
                                                            aaaGGATTTGTCAAATGATGTAGAATCCGGTCCCTCGTTGGGCGCGGTGGAGGAGTTAACATGTCAGAACGCGAAGGGGTCTTAGCAAGGGCGCCGCTTATCTATGCGTTGAGCGTAATCAGATTTGCCCCGATTCTTAAGCTGCCa                                                                                         >  3:259170/1‑147 (MQ=255)
                                                             aaGGATTTGTCAAATGATGTAGAATCCGGTCCCTCGTTGGGCGCGGTGGAGGAGTTAACATGTCAGAACGCGAAGGGGTCTTAGCAAGGGCGCCGCTTATCTATGCGTTGAGCGTAATCAGATTTGCCCCGATTCTTAAGCTGCCAAAg                                                                                      >  5:102832/1‑149 (MQ=255)
                                                              aGGATTTGTCAAATGATGTAGAATCCGGTCCCTCGTTGGGCGCGGTGGAGGAGTTAACATGTCAGAACGCGAAGGGGTCTTAGCAAGGGCGCCGCTTATCTATGCGTTGAGCGTAATCAGATTTGCCCCGATTCTTAAGCTGCCAAAGc                                                                                     <  7:62040/149‑1 (MQ=255)
                                                                  tttGTCAAATGATGTAGAATCCGGTCCCTCGTTGGGCGCGGTGGAGGAGTTAACATGTCAGAACGCGAAGGGGTCTTAGCAAGGGCGCCGCTTATCTATGCGTTGAGCGTAATCAGATTTGCCCCGATTCTTAAGCTGCCAAAGCTGa                                                                                  <  1:255402/148‑1 (MQ=255)
                                                                      tCAAATGATGTAGAATCCGGTCCCTCGTTGGGCGCGGTGGAGGAGTTAACATGTCAGAACGCGAAGGGGTCTTAGCAAGGGCGCCGCTTATCTATGCGTTGAGCGTAATCAGATTTGCCCCGATTCTTAAGCTGCCAAAGCTGATcc                                                                               >  1:156404/1‑147 (MQ=255)
                                                                          atgatgTAGAATCCGGTCCCTCGTTGGGCGCGGTGGAGGAGTTAACATGTCAGAACGCGAAGGGGGCTTAGGAAGGGCGCCGCTTATCTATGCGTTGAGCGTAATCAGATTTGCCGCGATTCTTAAGCTGCCAAAGCTGATCCCAGACa                                                                         <  7:264591/149‑1 (MQ=255)
                                                                             atgTAGAATCCGGTCCCTCGTTGGGCGCGGTGGAGGAGTTAACATGTCAGAACGCGAAGGGGTCTTAGCAAGGGCGCCGCTTATCTATGCGTTGAGCGTAATCAGATTTGCCCCGATTCTTAAGCTGCCAAAGCt                                                                                    <  6:237048/135‑1 (MQ=255)
                                                                             atgTAGAATCCGGTCCCTCGTTGGGCGCGGTGGAGGAGTTAACATGTCAGAACGCGAAGGGGTCTTAGCAAGGGCGCCGCTTATCTATGCGTTGAGCGTAATCAGATTTGCCCCGATTCTTAAGCTGCCAAAGCt                                                                                    >  5:237048/1‑135 (MQ=255)
                                                                             atgTAGAATCCGGTCCCTCGTTGGGCGCGGTGGAGGAGTTAACATGTCAGAACGCGAAGGGGTCTTAGCAAGGGCGCCGCTTATCTATGCGTTGAGCGTAATCAGATTTGCCCCGATTCTTAAGCTGCCAAAGCTGATCCCAGACAtt                                                                       <  3:144989/148‑1 (MQ=255)
                                                                                tAGAATCCGGTCCCTGGTTGGGCGCGGTGGAGGAGTTAACATGTCAGAACGCGAAGGGGTCTTAGCAAGGGCGCCGCTTATCTATGCGTTGAGCGTAATCAGATTTGCCCCGATTCTTAAGCTGCCAAAGCTGATCCCAGACATTCAGc                                                                   <  4:323010/149‑1 (MQ=255)
                                                                                        ggTCCCTCGTTGGGCGCGGTGGAGGAGTTAACATGTCAGAACGCGAAGGGGTCTTAGCAAGGGCGCCGCTTATCTATGCGTTGAGCGTAATCAGATTTGCCCCGATTCTTAAGCTGCCAAAGCTGATCCCAGACATTCAGCACACGATc                                                           >  8:76801/1‑149 (MQ=255)
                                                                                         gTCCCTCGTTGGGCGCGGTGGTGGAGTTAACATGTCAGAACGCGAAGGGGGCTTAGCAAGGGCGCCGCTTATCTATGCGTTGAGCGTAATCAGATTTGCCCCGATTCTTAAGCTGCCAAAGCTGATCCCAGACATTCAGCACACGATcc                                                          <  7:161496/149‑1 (MQ=255)
                                                                                         gTCCCTCGTTGGGCGCGGTGGAGGAGTTAACATGTCAGAACGCGAAGGGGTCTGAGCAAGGGCGCCGCTTATCTATGCGTTGAGCGTAATCAGATTTGCCCCGATTCTTAAGCTGCCAAAGCTGATCCCAGACATTCAGCACACGATcc                                                          >  4:312498/1‑149 (MQ=255)
                                                                                             cTCGTTGGGCGCGGTGGAGGAGTTAACATGTCAGAACGCGAAGGGGTCTTAGCAAGGGCGCCGCTTATCTATGCGTTGAGCGTAATCAGATTTGCCCCGATTCTTAAGCTGCCAAAGCTGATCCCAGACATTCa                                                                     >  1:264845/1‑134 (MQ=255)
                                                                                             cTCGTTGGGCGCGGTGGAGGAGTTAACATGTCAGAACGCGAAGGGGTCTTAGCAAGGGCGCCGCTTATCTATGCGTTGAGCGTAATCAGATTTGCCCCGATTCTTAAGCTGCCAAAGCTGATCCCAGACATTCa                                                                     <  2:264845/134‑1 (MQ=255)
                                                                                             cTCGTTGGGCGCGGTGGAGGAGTTAACATGTCAGAACGCGAAGGGGTCTTAGCAAGGGCGCCGCTTATCTATGCGTTGAGCGTAATCAGATTTGCCCCGATTCTTAAGCTGCCAAAGCTGATCCCAGACATTCAGCACACGATCCGTCa                                                      <  2:46112/149‑1 (MQ=255)
                                                                                              tCGTTGGGCGCGGTGGAGGAGTTAACATGTCAGAACGCGAAGGGGTCTTAGCAAGGGCGCCGCTTATCTATGCGTTGAGCGTAATCAGATTTGCCCCGATTCTTAAGCTGCCAAAGCTGATCCCAGACATTCAGCACACGATCCGt                                                        >  5:151758/1‑146 (MQ=255)
                                                                                                gTTGGGCGCTGTGGAGGAGTTAACATGTCAGAACGCGAAGGGGTCTTAGCAAGGGCGCCGCTTATCTATGCGTTGAGCGTAATCAGATTTGCCCCGATTCTTAAGCTGCCAAAGCTGATCCCAGACATTCAGCACACGATCCGTCaga                                                    <  2:176704/148‑1 (MQ=255)
                                                                                                gTTGGGCGCGGTGGAGGAGTTAACATGTCAGAACGCGAAGGGGTCTTAGCAAGGGCGCCGCTTATCTATGCGTTGAGCGTAATCAGATTTGCCCCGATTCTTAAGCTGCCAAAGCTGATCCCAGACATTCAGCACACGATCCGTCagag                                                   >  3:56064/1‑149 (MQ=255)
                                                                                                gTTGGGCGCGGTGGAGGAGTTAACATGTCAGAACGCGAAGGGGTCTTAGCAAGGGCGCCGCTTATCTATGCGTTGAGCGTAATCAGATTTGCCCCGATTCTTAAGCTGCCAAAGCTGATCCCAGACATTCAGCACACGATCCGTCaga                                                    <  3:1812/148‑1 (MQ=255)
                                                                                                   gggCGCGGTGGAGGAGTTAACATGTCAGAACGCGAAGGGGTCTTAGCAAGGGCGCCGCTTATCTATGCGTTGAGCGTAATCAGATTTGCCCCGATTCTTAAGCTGCCAAAGCTGATCCCAGACATTCAGCACACGATCCGTCAGAGCCt                                                >  5:31468/1‑149 (MQ=255)
                                                                                                       gcgGTGGAGGAGTTAACATGTCAGAACGCGAAGGGGTCTTAGCAAGGGCGCCGCTTATCTATGCGTTGAGCGTAATCAGATTTGCCCCGATTCTTAAGCTGCCAAAGCTGATCCCAGACATTCAGCACACGATCCGTCAGAGCCTCCCg                                            <  1:312831/149‑1 (MQ=255)
                                                                                                             gaggagTTAACATGTCAGAACGCGAAGGGGTCTTAGCAAGGGCGCCGCTTATCTATGCGTTGAGCGTAATCAGATTTGCCCCGATTCTTAAGCTGCCAAAGCTGATCCCAGACATTCAGCACACGATCCGTCAGAGCCTCCCGGGcttc                                      <  4:56064/149‑1 (MQ=255)
                                                                                                                gagTTACCCTGTCAGTACGCGAAGGGCTCTTAGCAAGGGCGCCGCTTAGCTATGCGTTGCGCGTAATCAGATTTGCCCCGATTCTTAAGCTGCCAAAGCTGATCCCAGACATTCAGCAGACGATCCGTCAGAGCCTCCCGGGCttcttc                                   <  8:142650/149‑1 (MQ=255)
                                                                                                                gagTTAACATGTCAGAACGCGAAGGGGTCTTAGCAAGGGCGCCGCTTATCTATGCGTTGAGCGTAATCAGATTTGCCCCGATTCTTAAGCTGCCAAAGCTGATCCCAGACATTCAGCACACGATCCGTCAGAGCCTCCCGGGCttcttc                                   <  4:259170/149‑1 (MQ=255)
                                                                                                                  gTTAACATGTCAGAACGAGAAGGGGGCTTAGCAAGGGCGCCGCTTATCTATGCGTTGAGCGTAATCAGATTTGCCCCGATTCTTAAGCTGCCAAAGCTGATCCCAGACATTCAGCACACGATCCGTCAGAGCCTCCCGGGCTTCTTCCa                                 <  6:224401/149‑1 (MQ=255)
                                                                                                                   ttAACATGTCAGAACGCGAAGGGGTCTTAGCAAGGGCGCCGCTTATCTATGCGTTGAGCGTAATCAGATTTGCCCCGATTCTTAAGCTGCCAAAGCTGATCCCAGACATTCAGCACACGATCCGTCAGAGCCTCCCGGGCttcttc                                   >  8:243998/1‑146 (MQ=255)
                                                                                                                     aaCATGTCAGAACGCGAAGGGGTCTTAGCAAGGGCGCCGCTTATCTATGCGTTGAGCGTAATCAGATTTGCCCCGATTCTTAAGCTGCCAAAGCTGATCCCAGACATTCAGCACACGATCCGTCAGAGCCTCCCGGGCTTCTTCCAGa                               <  6:189014/148‑1 (MQ=255)
                                                                                                                     aaCATGTCAGAACGCGAAGGGGTCTTAGCAAGGGCGCCGCTTATCTATGCGTTGAGCGTAATCAGATTTGCCCCGATTCTTAAGCTGCCAAAGCTGATCCCAGACATTCAGCACACGATCCGTCAGAGCCTCCCGGGCTTCTTCCAGAt                              >  2:269037/1‑149 (MQ=255)
                                                                                                                      aCATGTCAGAACGCGAAGGGGTCTTAGCAAGGGCGCCGCTTATCTATGCGTTGAGCGTAATCAGATTTGCCCCGATTCTTAAGCTGCCAAAGCTGATCCCAGACATTCAGCACACGATCCGTCAGAGCCTCCCGGGCTTCTTCCAGATg                             <  6:144858/149‑1 (MQ=255)
                                                                                                                       cATGTCAGAACGCGAAGGGGTCTTAGCAAGGGCGCCGCTTATCTATGCGTTGAGCGTAATCAGATTTGCCCCGATTCTTAAGCTGCTAAAGCTGATCCCAGACATTCAGCACACGATCCGCCAGAGCCTCCCGGGCTTCTTCCAGATgg                            <  4:66829/149‑1 (MQ=255)
                                                                                                                           tCAGAACGCGAAGGGGTCTTAGCAAGGGCGCCGCTTATCTATGCGTTGAGCGTAATCAGATTTGCCCCGATTCTTAAGCt                                                                                             <  6:155856/80‑1 (MQ=255)
                                                                                                                           tCAGAACGCGAAGGGGTCTTAGCAAGGGCGCCGCTTATCTATGCGTTGAGCGTAATCAGATTTGCCCCGATTCTTAAGCt                                                                                             >  5:155856/1‑80 (MQ=255)
                                                                                                                           tCAGAACGCGAAGGGGTCTTAGCAAGGGCGACGCTTATCTATGCGTTGAGCGTAATCAGATTTGCCCCGATTCTTAAGCTGCCAAAGCTGATCCCAGACATTCAGCACACGGTCCGTCAGAGCCTCCCGGGCTTCTTCCAGATGGTc                          >  1:31088/1‑147 (MQ=255)
                                                                                                                             aGAACGCGAAGGGGTCTTAGCAAGGGCGCCGCTTATCTATGCGTTGAGCGTAATCAGATTTGCCCCGATTCTTAAGCTGCCAAAGCTGATCCCAGACATTCAGCACACTATCCGTCAGAGCCTCCCTGGCTTCTTCCAGATGGTCAAgg                      <  2:156404/149‑1 (MQ=255)
                                                                                                                             aGAACGCGAAGGGGTCTTAGCAAGGGCGCCGCTTATCTATGCGTTGAGCGTAATCAGATTTGCCCCGATTCTTAAGCTGCCAAAGCTGATCCCAGACATTCAGCACACGATCCGTCAGAGCCTCCCGGGCTTCTTCCAGATGGTCAAgg                      >  5:344144/1‑149 (MQ=255)
                                                                                                                             aGAACGCGAAGGGGTCTTAGCAAGGGCGCCGCTTATCTATGCGTTGAGCGTAATCAGATTTGCCCCGATTCTTAAGCTGCCAAAGCTGATCCCAGACATTCAGCACACGATCCGTCAGAGCCTCCCGGGCTTCTTCCAGATGGTCAAgg                      <  4:68487/149‑1 (MQ=255)
                                                                                                                              gAACGCGAAGGGGTCTTAGCAAGGGCGCCGCTTATCTATGCGTTGAGCGTAATCAGATTTGCCCCGATTCTTAAGCTGCCAAAGCTGATCCCAGACATTCAGCACACGATCCGTCAGAGCCTCCCGGGCTTCTTCCAGATGGTCAAggg                     >  1:104867/1‑149 (MQ=255)
                                                                                                                                 cgcgAAGGGGTCTTAGCAAGGGCGCCGCTTATCTATGCGTTGAGCGTAATCAGATTTGCCCCGATTCTTAAGCTGCCAAAGCTGATCCCAGACATTCAGCACACGATCCGTCAGAGCCTCCCGGGCTTCTTCCAGATGGTCAAGGGCGt                  <  6:102832/149‑1 (MQ=255)
                                                                                                                                  gcgAAGGGGTCTTAGCAAGGGCGCCGCTTATCTATGCGTTGAGCGTAATCAGATTTGCCCCGATTCTTAAGCTGCCAAAGCTGATCCCAGACATTCAGCACACGATCCGTCAGAGCCTCCCGGGCTTCTTCCAGATGGTCAAGGGCGTg                 <  6:31468/149‑1 (MQ=255)
                                                                                                                                  gcgAAGGGGTCTTAGCAAGGGCGCCGCTTATCTATGCGTTGAGCGTAATCAGAATTACCCCGATTATTAAGCTGCCAAAGCTGATCCCAGACATTCAGCACACGATCCGTCAGAGACTCCCGGGCTTCATCCAGATGGTCAAGGGCGTg                 >  1:92944/1‑149 (MQ=255)
                                                                                                                                           tCTTAGCAAGGGCGCCGCTTATCTATGCGTTGAGCGTAATCAGATTTGCCCCGATTCTTAAGCTGCCAAAGCTGATCCCAGACATTCAGCACACGATCCGTCAGAGCCTCCCGGGCTTCTTCCAGATGGTCAAGGGCGTGCCGCCTggg        <  6:344144/149‑1 (MQ=255)
                                                                                                                                           tCTTAGCAAGGGCGCCGCTTATCTATGCGTTGAGCGTAATCAGATTTGCCCCGATTCTTAAGCTGCCAAAGCTGATCCCAGACATTCAGCACACGATCCGTCAGAGCCTCCCGGGCTTCTTCCAGATGGTCAAGGGCGTGCCGCCTggg        <  2:104867/149‑1 (MQ=255)
                                                                                                                                                 cAAGGGCGCCGCTTATCTATGCGTTGAGCGTAATCAGATTTGCCCCGATTCTTAAGCTGCCAAAGCTGATCCCAGACATTCAGCACACGATCCGTCAGAGCCTCCCGGGCTTCTTCCAGATGGTCAAGGGCGTGCCGCCTGGGGTGATg  <  6:151758/149‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
ACTCCATCCCTCACGGGGACCGCTTGTCAAATCGAAAGGCCCTGCTATACAGTTCTTGTCAAAGGATTTGTCAAATGATGTAGAATCCGGTCCCTCGTTGGGCGCGGTGGAGGAGTTAACATGTCAGAACGCGAAGGGGTCTTAGCAAGGGCGCCGCTTATCTATGCGTTGAGCGTAATCAGATTTGCCCCGATTCTTAAGCTGCCAAAGCTGATCCCAGACATTCAGCACACGATCCGTCAGAGCCTCCCGGGCTTCTTCCAGATGGTCAAGGGCGTGCCGCCTGGGGTGATG  >  NC_002947/23056‑23349

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑