| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 2,958,803 | G→C | 13.3% | intergenic (+69/+73) | PP_2589 → / ← PP_2590 | aldehyde dehydrogenase family protein/ferric siderophore receptor |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 2,958,803 | 0 | G | C | 13.3% | 78.6 / 5.0 | 30 | intergenic (+69/+73) | PP_2589/PP_2590 | aldehyde dehydrogenase family protein/ferric siderophore receptor |
| Reads supporting (aligned to +/- strand): ref base G (14/12); new base C (2/2); total (16/14) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.91e-01 | |||||||||||
CGATCTGTCACTGCATTCGTTCGACAAGTATTCGCAACTCAAGACCACCTGGTACCAGTTGCGCGGCTGATGTATTGATCATTGACCGCGTCGTCTGCTTCGCGGGCATGCCCGCTCCCACAGGTTCAGCACAAGT‑CTGAGGGTTGTGCTGGACCTGTGGGAGCGGGCGCGCCCGCGAAGAGGCCGTATCTGGCGCCGCGGCGGGACCCGGTTAGAAGTCCACGCTAGCCGAAAGCTGATAGGTCCGCGGCGCCCCCAACCCCAGGGTCTGGGTCAGTGGCA > NC_002947/2958665‑2958946 | cgATCTGTCACTGCATTCGTTCGACAAGTATTCGCAACTCAAGACCACCTGGTACCAGGTGCGCGGCTGATGTATTGATCATTGACCGCGTCGTCTGCTTCGCGGGCATGCCCGCTCCCACAGGTTCAGCACAAGT‑CTGAGGGTTGTGc < 1:176995/149‑1 (MQ=255) aCTGCATTCGTTCGACAAGTATTCGCAACTCAAGACCACCTGGTACCAGTTGCGCGGCTGATGTATTGATCATTGACCGCGTCGTCTGCTTCGCGGGCGCGCCCGCTCCCACAGGTCCAGCACAACC‑CTCAGACTTGTGCTGAACCtgt < 5:281010/149‑1 (MQ=25) aCTGCATTCGTTCGACAAGTATTCGCAACTCAAGACCACCTGGTACCAGTTGCGCGGCTGATGTATTGATCATTGACCGCGTCGTCTGCTTCGCGGGCATGCCCGCTCCCACAGGTTCAGCACAAGT‑CTGAGGGTTGTGCTGGACCtgt < 1:315743/149‑1 (MQ=255) aCTGCATTCGTTCGACAAGTATTCGCAACTCAAGACCACCTGGTACCAGTTGCGCGGCTGATGTATTGATCATTGACCGCGTCGTCTGCTTCGCGGGCATGCCCGCTCCCACAGGTTCAGCACAAGT‑CTGAGGGTTGTGCTGGACCtgt < 7:136813/149‑1 (MQ=255) cTGCATTCGTTCGACAAGTATTCGCAACTCAAGACCACCTGGTACCAGTTGCGCGGCTGATGTATTGATCATTGACCGCGTCGTCTGCTTCGCGGGCATGCCCGCTCCCACAGGTTCAGCACAAGT‑CTGAGGGTTGTGCTGGACCtgtg < 5:336772/149‑1 (MQ=255) gttcgACAAGTATTCGCAACTCAAGACCACCTGGTACCAGTTGCGCGGCTGATGTATTGATCATTGACCGCGTCGTCTGCTTCGCGGGCGCGCCCGCTCCCACAGGTCCAGCACAACC‑CTCAGACTTGTGCTGAAc > 8:179186/1‑136 (MQ=25) gttcgACAAGTATTCGCAACTCAAGACCACCTGGTACCAGTTGCGCGGCTGATGTATTGATCATTGACCGCGTCGTCTGCTTCGCGGGCGCGCCCGCTCCCACAGGTCCAGCACAACC‑CTCAGACTTGTGCTGAAc < 7:179186/136‑1 (MQ=25) ttcgACAAGTATTCGCAACTCAAGACCACCTGGTACCAGTTGCGCGGCTGATGTATTGATCATTGACCGCGTCGTCTGCTTCGCGGGCATGCCCGCTCCCACAGGTTCAGCACAAGT‑CTGAGGGTTGTGCTGGACCTGTGGGAGCGGgc < 6:53837/149‑1 (MQ=255) aCAAGTATTCGCAACTCAAGACCACCTGGTACCAGTTGCGCGGCTGATGTATTGATCATTGACCGCGTCGGCTGCTTCGCGGGCATGCCCGCTCCCACAGGTTCAGCACAAGT‑CTGAGGGTTGTGCTGGACCTGTGGGAGCGGgcgcgc < 3:135312/149‑1 (MQ=255) gTATTCGCAACTCAAGACCACCTGGTACCAGTTGCGCGGCTGATGTATTGATCATTGACCGCGTCGTCTGCTTCGCGGGCATGCCCGCTCCCACAGGTTCAGCACAAGT‑CTGa < 2:333257/113‑1 (MQ=255) gTATTCGCAACTCAAGACCACCTGGTACCAGTTGCGCGGCTGATGTATTGATCATTGACCGCGTCGTCTGCTTCGCGGGCATGCCCGCTCCCACAGGTTCAGCACAAGT‑CTGa > 1:333257/1‑113 (MQ=255) aaCTCAAGACCACCTGGTACCAGTTGCGCGGCTGATGTATTGATCATTGACCGCGTCGTCTGCTTCGCGGGCATGCCCGCTCCCACAGGTTCAGCACAAGT‑CTGAGGGTTGTGCTGGACCTGTGGGAGCGGGCGCGCCCGCGAAGAGGc > 4:67942/1‑149 (MQ=255) gTACCAGTTGCGCGGCTGATGTATTGATCATTGACCGCGTCGTCTGCTTCGCGGGCATGCCCGCTCCCACAGGTTCAGCACAAGT‑CTGAGGGTTGTGCTGGACCTGTGGGAGCGGGCGCGCCCGCGAAGAGGCCGTATCTGGCGCcgcg > 8:222666/1‑149 (MQ=255) ttGCGCGCCTGATGTATTGATCATTGACCGCGTCGTCTGCTTCGCGGGCATGCCCGCGCCCACAGGTTCACCACAAGT‑CTGAGGGTTGTGCTGGACCTGTGGGAGCGGGCGCGCCCGCGAAGAGGCCGTATCTGGCGCCGCGGCGGGAc < 5:339118/149‑1 (MQ=255) tCATTGACCGCGTCGTCTGCTTCGCGGGCATGCCCGCTCCCACAGGTTCAGCACAAGT‑CTGAGGGTTGTGCTGGACCTGTGGGAGCGGGCGCGCCCGCGAAGAGGCCGTATCTGGCGCCGCGGCGGGACCCGGTTAGAAGTCCACGc > 3:66887/1‑147 (MQ=255) aTTGACCGCGTCGTCTGCTTCGCGGGCATGCCCGCTCCCACAGGTTCAGCACAAGT‑CTGAGGGTTGTGCTGGACCTGTGGGAGCGGGCGCGCCCGCGAAGAGGCCGTATCTGGCGCCGCGGCGGGACCCGGTTAGAAGTCCACGCTAg < 3:67942/148‑1 (MQ=255) cgtcTGCTTCGCGGGCATGCCCGCTCCCACAGGTTCAGCACAAGT‑CTGAGGGTTGTGCTGGACCTGTGGGAGCGGGCGCGCCCGCGAAGAGGCCGTATCTGGCGCCGCGGCGGGACCCGGTTAGAAGTCCACGCTAGCCGAAAGCTGAt < 7:222666/149‑1 (MQ=255) ttCGCGGGCATGCCCGCTCCCACAGGTTCAGCACAAGT‑CTGAGGGTTGTGCTGGACCTGTGGGAGCGGGCGCGCCCGCGAAGAGGCCGTATCTGGCGCCGCGGCGGGACCCGGTTAGAAGTCCACGCTAGCCGAAAGCTGATAGGTCcg > 8:84010/1‑149 (MQ=255) gcgGGCATGCCCGCTCCCACAGGTTCAGCACAAGT‑CTGAGGGTTGTGCTGGACCTGTGGGAGCGGGCGCGCCCGCGAAGAGGCCGTATCTGGCGCCGCGGCGGGACCCGGTTAGAAGTCCACGCTAGCCGACAGCTGATAGGTCCgcgg > 4:84928/1‑149 (MQ=255) aTGCCCGCTCCCACAGGTTCAGCACAAGTACTGAGGGTTGTGCTGGACCTGTGGGAGCGGGCGCGCCCGCGAAGAGGCCGTATCTGGCGCCGCGGCGGGACCCGGTTAGAAGTCCACGCTAGCCGAAAGCTGATAGGTCCGCGGCGccc > 1:198847/1‑149 (MQ=255) cTCCCACAGGTCCAGCACAACC‑CTCAGACTTGTGCTGAACCTGTGGGAGCGGGCATGCCCGCGAAGAGGCCGGATCTGGCGCCGCGGCGGGACCCGGGTAGAAGTCCACGCTAGCCGAAAGCTGATAGGTCCGCGGCGCCCCCACcccc > 3:98350/1‑149 (MQ=21) ccACAGGTTCAGCACAAGT‑CTGAGGGTTGTGCTGGACCTGTGGGAGCGGGCGCGCCCGCGAAGAGGCCGTATCTGGCGCCGCGGCGGGACCCGGTTAGAAGTCCACGCTAGCCGAAAGCTGATAGGTCCGCGGCGCCCCCAACCCCAgg > 5:179690/1‑149 (MQ=255) ccACAGGTTCAGCACAAGT‑CTGAGGGTTGTGCTGGACCTGTGGGAGCGGGCGCGCCCGCGAAGAGGCCGTATCTGGCGCCGCGGCGGGACCCGGTTAGAAGTCCACGCTAGCCGAAAGCTGATAGGTCCGCGGCGCCCCCAACCCCAgg > 2:219313/1‑149 (MQ=255) acaGGTTCAGCACAAGT‑CTGAGGGTTGTGCTGGACCTGTGGGAGCGGGCGCGCCCGCGAAGAGGCCGTATCTGGCGCCGCGGCGGGACCCGGTTAGAAGTCCACGCTAGCCGAAAGCTGATAGGTCCGCGGCGCCCCCAACCCCAGGGt > 5:236016/1‑149 (MQ=255) tCAGCACAAGT‑CTGAGGGTTGTGCTGGACCTGTGGGAGCGGGCGCGCCCGCGAAGAGGCCGTATCTGGCGCCGCGGCGGGACCCGGTTAGAAGTCCACGCTAGCCGAAAGCTGATAGGTCCGCGGCGCCCCCAACCCCAGGGTCTGGGt > 3:213286/1‑149 (MQ=255) aGT‑CTGAGGGTTGTGCTGGACCTGTGGGAGCGGGCGCGCCCGCGAAGAGGCCGTATCTGGCGCCGCGGCGGGACCCGGTTAGAAGTCCACGCTAGCCGAAAGCTGATAGGTCCGCGGCGCCCCCAACCCCAGGGTCTGGGTCAGTGGca > 1:88961/1‑149 (MQ=255) gT‑CTGAGGGTTGTGCTGGACCTGTGGGAGCGGGCGCGCCCGCGAAGAGGCCGTATCTGGCGCCGCGGCGGGACCCGGTTa > 6:149739/1‑80 (MQ=255) gT‑CTGAGGGTTGTGCTGGACCTGTGGGAGCGGGCGCGCCCGCGAAGAGGCCGTATCTGGCGCCGCGGCGGGACCCGGTTa < 5:149739/80‑1 (MQ=255) cTGAGGGTTGTGCTGGACCTGTGGGAGCGGGCGCGCCCGCGAAGAGGCCGTATCTgg < 6:141046/57‑1 (MQ=255) cTGAGGGTTGTGCTGGACCTGTGGGAGCGGGCGCGCCCGCGAAGAGGCCGTATCTgg > 5:141046/1‑57 (MQ=255) | CGATCTGTCACTGCATTCGTTCGACAAGTATTCGCAACTCAAGACCACCTGGTACCAGTTGCGCGGCTGATGTATTGATCATTGACCGCGTCGTCTGCTTCGCGGGCATGCCCGCTCCCACAGGTTCAGCACAAGT‑CTGAGGGTTGTGCTGGACCTGTGGGAGCGGGCGCGCCCGCGAAGAGGCCGTATCTGGCGCCGCGGCGGGACCCGGTTAGAAGTCCACGCTAGCCGAAAGCTGATAGGTCCGCGGCGCCCCCAACCCCAGGGTCTGGGTCAGTGGCA > NC_002947/2958665‑2958946 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |