Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,030,515 C→A 8.3% intergenic (+515/‑179) pcaY → / → mgtA aromatic acid chemoreceptor/ATP‑dependent magnesium transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,030,5150CA 8.3% 148.1 / 4.2 48intergenic (+515/‑179)pcaY/mgtAaromatic acid chemoreceptor/ATP‑dependent magnesium transporter
Reads supporting (aligned to +/- strand):  ref base C (25/19);  new base A (2/2);  total (27/21)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.93e-01

CTGGCCGCGGCGTCTGCACGGCTACCTTGACCACCACCAGCACCCCCCAAGTAGTTGCGGGGTGCTGGCGCGTCTTCATGCACGCTTGAGCCCCCGCACTTCTCACTGAATAAAAGATATTGAACGGTATCTTTGTACTCGAACTTTTCATGTTAAAAGTTCGCGGGATAACTATTTCCCTGTGTTTATGAAAAGGAATCAAACCATGGATTGCGCGAGTAGAAAGTTCGCTGCCGACACAACTTCCGACTCTCGGCAGCGGACAGACAACTGTCGGAAAGAACCAGCATTATTCAG  >  NC_002947/3030367‑3030663
                                                                                                                                                    |                                                                                                                                                    
cTGGCCGCGGCGTCTGCACGGCTACCTTGACCACCACCAGCACCCCCCAAGTAGTTGCGGGGTGCTGGCGCGTCTTCATGCACGCTTGAGCCCCCGCACTTCTCACTGAATAAAAGATATTGAACGGTATCTTTGTACTCGAACTTTTc                                                                                                                                                      <  4:150924/149‑1 (MQ=255)
         gcgTCTGCACGGCTACCTTGACCACCACCAGCACCCCCCAAGTAGTTGCGGGGTGCTGGCGCGTCTTCATGCACGCTTGAGCCCCCGCACTTCTCACTGAATAAAAGATATTGAACGGTATCTTTGTACTCGAACTTTTCATGTTaaaa                                                                                                                                             <  5:267611/149‑1 (MQ=255)
           gTCTGCACGGCTACCTTGACCACCACCAGCACCCCCCAAGTAGTTGCGGGGTGCTGGCGCGTCTTCATGCACGCTTGAGCCCCCGCACTTCTCACTGAATAAAAGATATTGAACGGTATCTTTGTACTCGAACTTTTCATGTTAAAAGt                                                                                                                                           >  2:297466/1‑149 (MQ=255)
            tCTGCACGGCTACCTTGACCACCACCAGCACCCCCCAAGTAGTTGCGGGGTGCTGGCGCGTCTTCATGCACGCTTGAGCCCCCGCACTTCTCACTGAATAAAAGATATTGAACGGTATCTTTGTACTCGAACTTTTCATGTTAAAAGtt                                                                                                                                          >  5:277561/1‑149 (MQ=255)
               gCACGGCTACCTTGACCACCACCAGCACCCCCCAAGTAGTTGCGGGGTGCTGGCGCGTCTTCATGCACGCTTGAGCCCCCGCACTTCTCACTGAATAAAAGATATTGAACGGTATCTTTGTACTCGAACTTTTCATGTTAAAAGTTcgc                                                                                                                                       >  4:93211/1‑149 (MQ=255)
                cACGGCTACCTTGACCACCACCAGCACCCCCCAAGTAGTTGCGGGGTGCTGGCGCGTCTTCATGCACGCTTGAGCCCCCGCACTTCTCACTGAATAAAAGATATTGAACGGTATCTTTGTACTCGAACTTTTCATGTTAAAAGTTcgcg                                                                                                                                      <  1:124525/149‑1 (MQ=255)
                cACGGCTACCTTGACCACCACCAGCACCCCCCAAGTAGTTGCGGGGTGCTGGCGCGTCTTCATGCACGCTTGAGCCCCCGCACTTCTCACTGAATAAAAGATATTGAACGGTATCTTTGTACTCGAACTTTTCATGTTAAAAGTTcgcg                                                                                                                                      >  8:143906/1‑149 (MQ=255)
                              ccaccaccaGCACCCCCCAAGTAGTTGCGGGGTGCTGGCGCGTCTTCATGCACGCTTGAGCCCCCGCACTTCTCACTGAATAAAAGATATTGAACGGTATCTTTGTACTCGAACTTTTCATGTTAAAAGTTCGCGGGATAACTATTTcc                                                                                                                        <  1:297466/149‑1 (MQ=255)
                              ccaccaccaGCACCCCCCAAGTAGTTGCGGGGTGCTGGCGCGTCTTCATGCACGCTTGAGCCCCCGCACTTCTCACTGAATAAAAGATATTGAACGGTATCTTTGTACTCGAACTTTTCATGTTAAAAGTTCGCGGGATAACTATTTcc                                                                                                                        <  6:221975/149‑1 (MQ=255)
                                accaccaGCACCCCCCAAGTAGTTGCGGGGTGCTGGCGAGTCTTCATGCACTCATGAGCCCCCGCACTTCTCACTGAATAAAAGATATTGAACGGTATCTTTGTACTCGAACTTTTCATGTTAAAAGTTCGCGGGATAACTATTTCCCt                                                                                                                      >  6:20023/1‑149 (MQ=255)
                                 gcgccaGCACCCCCCAAGTAGTTGCGGGGTGCTGGCGCGTCTTCATGCACGCTTGAGCCCCCGCACTTCTCACTGAATAAAAGATATTGAACGGTATCTTTGTACTCGAACTTTTCATGTTAAAAGTTCGCGGGATAACTATTTCCCtg                                                                                                                     >  6:83732/4‑149 (MQ=255)
                                  caccaGCACCCCCCAAGTAGTTGCGGGGTGCTGGCGCGTCTTCATGCACGCTTGAGCCCCCGCACTTCTCACTGAATAAAAGATATTGAACGGTATCTTTGTACTCGAACTTTTCATGTTAAAAGTTCGCGGGATAACTATTTCCCtgt                                                                                                                    >  2:287159/1‑149 (MQ=255)
                                  caccaGCACCCCCCAAGTAGTTGCGGGGTGCTGGCGCGTCTTCATGCACGCTTGAGCCCCCGCACTTCTCACTGAATAAAAGATATTGAACGGTATCTTTGTACTCGAACTTTTCATGTTAAAAGTTCGCGGGATAACTATTTCCCtgt                                                                                                                    <  2:287944/149‑1 (MQ=255)
                                   gccaGCACCCCGCAACTACTTGGGGGGTGCTGGTGCGTCTTCATGCACGCTTGAGCCCCCGCACTTCTCACTGAATAAAAGATATTGAACGGTATCTTTGTACTCGAACTTTTCATGTTAAAAGTTCGCGGGATAACTATTTCCCtgtg                                                                                                                   >  4:116899/2‑149 (MQ=39)
                                   gccaGCACCCCGCAACTACTTGGGGGGTGCTGGCGCGTCTTCATGCACGCTTGCG‑CCCCGCACTTCTCACTGAATAAAAGATATTGAACGGTATCTTTGTACTCGAACTTTTCATGTTAAAAGTTCGCGGGATAACTATTTCCCTgtgt                                                                                                                  >  6:319702/2‑149 (MQ=255)
                                    ccaGCACCCCCCAAGTAGTTGCGGGGTGCTGGCGCGTCTTCATGCACGCTTGAGCCCCCGCACTTCTCACAGAATAAAAGATATTGAACGGTATCTTTGTACTCGAACTTTTAACATGAGAAGTTcgag                                                                                                                                      <  2:30181/129‑3 (MQ=255)
                                    ccaGCACCCCCCAAGTAGTTGCGGGGTGCTGGCGCGTCTTCATGCACGCTTGAGCCCCCGCACTTCTCACAGAATAAAAGATATTGAACGGTATCTTTGTACTCGAACTTTTAACATGAGAAGTTcgag                                                                                                                                      >  1:30181/1‑127 (MQ=255)
                                          ccccccAAGTAGTTGCGGGGTGCTGGCGCGTCTTCATGCACGCTTGAGCCCCCGCACTTCTCACTGAATAAAAGATATTGAACGGTATCTTTGTACTCGAACTTTTCATGTTAAAAGTTCGCGGGATAACTATTTCCCTGTGTTTATGa                                                                                                            <  7:143906/149‑1 (MQ=255)
                                             cccAAGTAGTTGCGGGGTGCTGGCGCGTCTTCATGCACGCTTGAGCCCCCGCACTTCTCACTGAATAAAAGATATTGAACGGTATCTTTGTACTCGAACTTTTCATGTTAAAAGTTCGCGGGATAACTATTTCCCTGTGTTTATGaaaa                                                                                                         <  6:277561/149‑1 (MQ=255)
                                              ccAAGTAGTTGCGGGGTGCTGGCGCGTCTTCATGCACGCTTGAGCCCCCGCACTTCTCACTGAATAAAAGATATTGAACGGTATCTTTGTACTCGAACTTTTCATGTTAAAAGTTCGCGGGATAACTATTTCCCTGTGTTTATGAAAAg                                                                                                        <  4:133738/149‑1 (MQ=255)
                                                aaGTAGTTGCGGGGTGCTGGCGCGTCTTCATGCACGCTTGAGCCCCCGCACTTCTCACTGAATAAAAGATATTGAACGGTATCTTTGTACTCGAACTTTTCATGTTAAAAGTTCGCGGGATAACTATTTCCCTGTGTTTATGAAAAgg                                                                                                       >  7:81348/1‑148 (MQ=255)
                                                 agtagtTGCGGGGTGCTGGCGCGTCTTCATGCACGCTTGAGCCCCCGCACTTCTCACTGAATAAAAGATATTGAACGGTATCTTTGTACTCGAACTTTTCATGTTAAAAGTTCGCGGGATAACTATTTCCCTGTGTTTATGAAAAGGaa                                                                                                     <  3:93211/149‑1 (MQ=255)
                                                   tagtTGCGGGGTGCTGGCGCGTCTTCATGCACGCTTGAGCCCCCGCACTTCTCACTGAATAAAAGATATTGAACGGTATCTTTGTACTCGAACTTTTAACATGAAAAGTTcga                                                                                                                                       <  7:83960/113‑2 (MQ=255)
                                                   tagtTGCGGGGTGCTGGCGCGTCTTCATGCACGCTTGAGCCCCCGCACTTCTCACTGAATAAAAGATATTGAACGGTATCTTTGTACTCGAACTTTTAACATGAAAAGTTcga                                                                                                                                       >  8:83960/1‑112 (MQ=255)
                                                                   gcgcGTCTTCATGCACGCTTGAGCCCCCGCACTTCTCACTGAATAAAAGATATTGAACGGTATCTTTGTACTCGAACTTTTCATGTTAAAAGTTCGCGGGATAACTATTTCCCTGTGTTTATGAAAAGGAATCAAACCATGGATTgcgc                                                                                   <  5:83732/149‑1 (MQ=255)
                                                                            cATGCACGCTTGAGCCCCCGCACTTCTCACTGAATAAAAGATATTGAACGGTATCTTTGTACTCGAACTTTTCATGTTAAAAGTTCGCGGGATAACTATTTCCCTGTGTTTATGAAAAGGAATCAAACCATGGATTGCGCGAGTAGaaa                                                                          >  2:202233/1‑149 (MQ=255)
                                                                             aTGCACGCTTGAGCCCCCGCACTTCTCACTGAATAAAAGATATTGAACGGTATCTTTGTACTCGAACTTTTCATGTTAAAAGTTCGCGGGATAACTATTTCCCTGTGTTTAt                                                                                                              <  8:21287/112‑1 (MQ=255)
                                                                             aTGCACGCTTGAGCCCCCGCACTTCTCACTGAATAAAAGATATTGAACGGTATCTTTGTACTCGAACTTTTCATGTTAAAAGTTCGCGGGATAACTATTTCCCTGTGTTTAt                                                                                                              >  7:21287/1‑112 (MQ=255)
                                                                                    cTTGAG‑CCCCGCACTTCTCACTGAATAAAAGATATTGAACGGTATCTTTGTACTCGAACTTTTCATGTTAAAAGTTCGCGGGATAACTATTTCCCTGTGTTTATGAAAAGGAATCAAACCATGGATTGCGCGAGTAGAAAGTTCGCTGc                                                                 <  5:319702/149‑1 (MQ=255)
                                                                                            cccGCACTTCTCACTGAATAAAAGATATTGAACGGTATCTTTGTACTCGAACTTTTCATGTTAAAAGTTCGCGGGATAACTATTTCCCTGTGTTTATGAAAAGGAATCAAACCATGGATTGCGCGAGTAGAAAGTTCGCTGCCGAcaca                                                          <  4:259525/149‑1 (MQ=255)
                                                                                             ccGCACTTCTCACTGAATAAAAGATATTGAACGGTATCTTTGTACTCGAACTTTTCATGTTAAAAGTTCGCGGGATAACTATTTCCCTGTGTTTATGAAAAGGAATCAAACCATGGATTGCGCGAGTAGAAAGTTCGCTGCCGACACaa                                                         >  1:217126/1‑149 (MQ=255)
                                                                                                 aCTTCTCACTGAATAAAAGATATTGAACGGTATCTTTGTACTCTAACTTTTCATGTTAAAAGTTCGCGGGATAACTATTTCCCTGTGTTTATGAAAAGGAATCAAACCATGGATTGCGCGAGTAGAAAGTTCGCTGCCGACACAACTTc                                                     <  5:20023/149‑1 (MQ=255)
                                                                                                        aCTGAATAAAAGATATTGAACGGTATCTTTGTACTCGAACTTTTCATGTTAAAAGTTCGCGGGATAACTATTTCCCTGTGTTTATGAAAAGGAATCAAACCATGGATTGCGCGa                                                                                 <  5:206261/114‑1 (MQ=255)
                                                                                                        aCTGAATAAAAGATATTGAACGGTATCTTTGTACTCGAACTTTTCATGTTAAAAGTTCGCGGGATAACTATTTCCCTGTGTTTATGAAAAGGAATCAAACCATGGATTGCGCGa                                                                                 >  6:206261/1‑114 (MQ=255)
                                                                                                         cTGAATAAAAGATATTGAACGGTATCTTTGTTCTCGAACTTTTCATGTTAAAAGTTCGCGGGATAACTATTTCCCTGTGTTTATGAAAAGGAATCAAACCATGGATTGCGCGAGTAGAAAGTTCGCTGCCGACACAACTTCCGACtctc                                             >  8:236179/1‑149 (MQ=255)
                                                                                                          tGAATAAAAGATATTGAACGGTATCTTTGTACTCGAACTTTTCATGTTAAAAGTTCGCGGGATAACTATTTCCCTGTGTTTATGAAAAGGAATCAAACCATGGATTGCGCGAGTAGAAAGTTCGCTGCCGACACAACTTCCGACTCTCg                                            >  5:310416/1‑149 (MQ=255)
                                                                                                            aaTAAAAGATATTGAACGGTATCTTTGTACTCGAACTTTTCATGTTAAAAGTTCGCGGGATAACTATTTCCCTGTGTTTATGAAAAGGAATCAAACCATGGATTGCGCGAGTAGAAAGTTCGCTGCCGACACAACTTCCGACTCTCgg                                           >  8:48133/1‑148 (MQ=255)
                                                                                                               aaaaGATATTGAACGGTATCTTTGTACTCGAACTTTTCATGTTAAAAGTTCGCGGGATAACTATTTCCCTGTGTTTATGAAAAGGAATCAAACCATGGATTGCGAGAGTAGAAAGTTCGCTGCCTACACAACTTCCGACTCTCTGCAGc                                       >  4:304201/1‑149 (MQ=255)
                                                                                                                aaaGATATTGAACGGTATCTTTGTACTCGAACTTTTCATGTT‑AAAGTTCGCGGGATAACTATTTCCCTGTGTTTATGAAAAGGAATCAAACCATGGATTGCGCGAGTAGAAAGTTCGCTGCCGACACAACTTCCGACTCTCGGCAGCg                                      >  7:80020/1‑148 (MQ=255)
                                                                                                                 aaGATATTGAACGGTATCTTTGTACTCGAACTTTTCATGTTAAAAGTTCGCGGGATAACTATTTCCCTGTGTTTATGAAAAGGAATCAAACCATGGATTGCGCGAGTAGAAAGTTCGCTGCCGACACAACTTCCGACTCTCGGCAGCg                                      >  4:129375/1‑148 (MQ=255)
                                                                                                                     tatTGAACGGTATCTTTGTACTCGAACTTTTCATGTTAAAAGTTCGCGGGATAACTATTTCCCTGTGTTTATGAAAAGGAATCAAACCATGGATTGCGCGAGTAGAAAGTTCGCTGCCGACACAACTTCCGACTCTCGGCAGCGgaca                                  <  6:310416/148‑1 (MQ=255)
                                                                                                                       ttGAACGGTATCTTTGTACTCGAACTTTTCATGTT‑AAAGTTCGCGGGATAACTATTTCCCTGTGTTTATGAAAAGGAATCAAACCATGGATTGCGCGAGTAGAAAGTTCGCTGCCGACACAACTTCCGACTCTCGGCAGCGgacagac                               <  8:80020/148‑1 (MQ=255)
                                                                                                                         gAACGGTATCTTTGTACTCGAACTTTTCATGTTAAAAGTTCGCGGGATAACTATTTCCCTGTGTTTATGAAAAGGAATCAAACCATGGATTGCGCGAGTAGAAAGTTCGCTGCCGACACAACTTCCGACTCTCGGCAGCGgacagaca                              >  2:12115/1‑148 (MQ=255)
                                                                                                                             ggTATCTTTGTACTCGAACTTTTCATGTTAAAAGTTCGCGGGATAACTATTTCCCTGTGTTTATGAAAAGGAATCAAACCATGGATTGCGCGAGTAGAAAGTTCGCTGCCGACACAACTTCCGACTCTCGGCAGCGGACAGACAACTGt                         >  4:166646/1‑149 (MQ=255)
                                                                                                                               tATCTTTGTACTCGAACTTTTCATGTTAAAAGTTCGCGGGATAACTATTTCCCTGTGTTTATGAAAAGGAATCAAACCATGGATTGCGCGAGTAGAAAGTTCGCTGCCGACACAACTTCCGACTCTCGGCAGCGGACAGACAACTGTCg                       >  6:264691/1‑149 (MQ=255)
                                                                                                                                     tGTACTCGAACTTTTCATGTTAAAAGTTCGCGGGATAACTATTTCCCTGTGTTTATGAAAAGGAATCAAACCATGGATTGCGCGAGTAGAAAGTTCGCTGCCGACACAACTTCCGACTCTCGGCAGCGGACAGACAACTGTCGGaaa                   <  3:166646/147‑1 (MQ=255)
                                                                                                                                          tCGAACTTTTCATGTTAAAAGTTCGCGGGATAACTATTTCCCTGTGTTTATGAAAAGGAATCAAACCATGGATTGCGCGAGTAGAAAGTTCGCTGCCGACACAACTTCCGACTCTCGGCAGCGGACAGACAACTGTCGGAAAGAACCAg            >  1:257604/1‑149 (MQ=255)
                                                                                                                                                ttttCATGTTAAAAGTTCGCGGGATAACTATTTCCCTGTGTTTATGAAAAGGAATCAAACCATGGATTGCGCGAGTAGAAAGTTCGCTGCCGACACAACTTCCGACTCTCGGCAGCGGACAGACAACTGTCGGAAAGAACCAGCattat      >  8:248828/1‑149 (MQ=255)
                                                                                                                                                  ttCATGTTAAAAGTTCGCGGGATAACTATTTCCCTGTGTTTATGAAAAGGAATCAAACCATGGATTGCGCGAGTAGAAAGTTCGCTGCCGACACAACTTCCGACTCTCGGCAGCGGACAGACAACTGTCGGAAAGAACCAGCattatt     <  8:81348/148‑1 (MQ=255)
                                                                                                                                                    cATGTTAAAAGTTCGCGGGATAACTATTTCCCTGTGTTTATGAAAAGGAATCAAACCATGGATTGCGCGAGTAGAAAGTTCGCTGCCGACACAACTTCCGACTCTCGGCAGCGGACAGACAACTGTCGG‑AAGAACCAGCATTATTCAg  <  2:217126/148‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
CTGGCCGCGGCGTCTGCACGGCTACCTTGACCACCACCAGCACCCCCCAAGTAGTTGCGGGGTGCTGGCGCGTCTTCATGCACGCTTGAGCCCCCGCACTTCTCACTGAATAAAAGATATTGAACGGTATCTTTGTACTCGAACTTTTCATGTTAAAAGTTCGCGGGATAACTATTTCCCTGTGTTTATGAAAAGGAATCAAACCATGGATTGCGCGAGTAGAAAGTTCGCTGCCGACACAACTTCCGACTCTCGGCAGCGGACAGACAACTGTCGGAAAGAACCAGCATTATTCAG  >  NC_002947/3030367‑3030663

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑