| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 3,030,518 | G→A | 8.1% | intergenic (+518/‑176) | pcaY → / → mgtA | aromatic acid chemoreceptor/ATP‑dependent magnesium transporter |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 3,030,518 | 0 | G | A | 8.1% | 139.4 / 2.3 | 49 | intergenic (+518/‑176) | pcaY/mgtA | aromatic acid chemoreceptor/ATP‑dependent magnesium transporter |
| Reads supporting (aligned to +/- strand): ref base G (25/20); new base A (2/2); total (27/22) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.93e-01 | |||||||||||
GCGTCTGCACGGCTACCTTGACCACCACCAGCACCCCCCAAGTAGTTGCGGGGTGCTGGCGCGTCTTCATGCACGCTTGAGCCCCCGCACTTCTCACTGAATAAAAGATATTGAACGGTATCTTTGTACTCGAACTTTTCATGTTAAAAGTTCGCGGGATAACTATTTCCCTGTGTTTATGAAAAGGAATCAAACCATGGATTGCGCGAGTAGAAAGTTCGCTGCCGACACAACTTCCGACTCTCGGCAGCGGACAGACAACTGTCGGAAAGAACCAGCATTATTCAG > NC_002947/3030376‑3030663 | gcgTCTGCACGGCTACCTTGACCACCACCAGCACCCCCCAAGTAGTTGCGGGGTGCTGGCGCGTCTTCATGCACGCTTGAGCCCCCGCACTTCTCACTGAATAAAAGATATTGAACGGTATCTTTGTACTCGAACTTTTCATGTTaaaa < 5:267611/149‑1 (MQ=255) gTCTGCACGGCTACCTTGACCACCACCAGCACCCCCCAAGTAGTTGCGGGGTGCTGGCGCGTCTTCATGCACGCTTGAGCCCCCGCACTTCTCACTGAATAAAAGATATTGAACGGTATCTTTGTACTCGAACTTTTCATGTTAAAAGt > 2:297466/1‑149 (MQ=255) tCTGCACGGCTACCTTGACCACCACCAGCACCCCCCAAGTAGTTGCGGGGTGCTGGCGCGTCTTCATGCACGCTTGAGCCCCCGCACTTCTCACTGAATAAAAGATATTGAACGGTATCTTTGTACTCGAACTTTTCATGTTAAAAGtt > 5:277561/1‑149 (MQ=255) gCACGGCTACCTTGACCACCACCAGCACCCCCCAAGTAGTTGCGGGGTGCTGGCGCGTCTTCATGCACGCTTGAGCCCCCGCACTTCTCACTGAATAAAAGATATTGAACGGTATCTTTGTACTCGAACTTTTCATGTTAAAAGTTcgc > 4:93211/1‑149 (MQ=255) cACGGCTACCTTGACCACCACCAGCACCCCCCAAGTAGTTGCGGGGTGCTGGCGCGTCTTCATGCACGCTTGAGCCCCCGCACTTCTCACTGAATAAAAGATATTGAACGGTATCTTTGTACTCGAACTTTTCATGTTAAAAGTTcgcg < 1:124525/149‑1 (MQ=255) cACGGCTACCTTGACCACCACCAGCACCCCCCAAGTAGTTGCGGGGTGCTGGCGCGTCTTCATGCACGCTTGAGCCCCCGCACTTCTCACTGAATAAAAGATATTGAACGGTATCTTTGTACTCGAACTTTTCATGTTAAAAGTTcgcg > 8:143906/1‑149 (MQ=255) ccaccaccaGCACCCCCCAAGTAGTTGCGGGGTGCTGGCGCGTCTTCATGCACGCTTGAGCCCCCGCACTTCTCACTGAATAAAAGATATTGAACGGTATCTTTGTACTCGAACTTTTCATGTTAAAAGTTCGCGGGATAACTATTTcc < 1:297466/149‑1 (MQ=255) ccaccaccaGCACCCCCCAAGTAGTTGCGGGGTGCTGGCGCGTCTTCATGCACGCTTGAGCCCCCGCACTTCTCACTGAATAAAAGATATTGAACGGTATCTTTGTACTCGAACTTTTCATGTTAAAAGTTCGCGGGATAACTATTTcc < 6:221975/149‑1 (MQ=255) accaccaGCACCCCCCAAGTAGTTGCGGGGTGCTGGCGAGTCTTCATGCACTCATGAGCCCCCGCACTTCTCACTGAATAAAAGATATTGAACGGTATCTTTGTACTCGAACTTTTCATGTTAAAAGTTCGCGGGATAACTATTTCCCt > 6:20023/1‑149 (MQ=255) gcgccaGCACCCCCCAAGTAGTTGCGGGGTGCTGGCGCGTCTTCATGCACGCTTGAGCCCCCGCACTTCTCACTGAATAAAAGATATTGAACGGTATCTTTGTACTCGAACTTTTCATGTTAAAAGTTCGCGGGATAACTATTTCCCtg > 6:83732/4‑149 (MQ=255) caccaGCACCCCCCAAGTAGTTGCGGGGTGCTGGCGCGTCTTCATGCACGCTTGAGCCCCCGCACTTCTCACTGAATAAAAGATATTGAACGGTATCTTTGTACTCGAACTTTTCATGTTAAAAGTTCGCGGGATAACTATTTCCCtgt > 2:287159/1‑149 (MQ=255) caccaGCACCCCCCAAGTAGTTGCGGGGTGCTGGCGCGTCTTCATGCACGCTTGAGCCCCCGCACTTCTCACTGAATAAAAGATATTGAACGGTATCTTTGTACTCGAACTTTTCATGTTAAAAGTTCGCGGGATAACTATTTCCCtgt < 2:287944/149‑1 (MQ=255) gccaGCACCCCGCAACTACTTGGGGGGTGCTGGTGCGTCTTCATGCACGCTTGAGCCCCCGCACTTCTCACTGAATAAAAGATATTGAACGGTATCTTTGTACTCGAACTTTTCATGTTAAAAGTTCGCGGGATAACTATTTCCCtgtg > 4:116899/2‑149 (MQ=39) gccaGCACCCCGCAACTACTTGGGGGGTGCTGGCGCGTCTTCATGCACGCTTGCG‑CCCCGCACTTCTCACTGAATAAAAGATATTGAACGGTATCTTTGTACTCGAACTTTTCATGTTAAAAGTTCGCGGGATAACTATTTCCCTgtgt > 6:319702/2‑149 (MQ=255) ccaGCACCCCCCAAGTAGTTGCGGGGTGCTGGCGCGTCTTCATGCACGCTTGAGCCCCCGCACTTCTCACAGAATAAAAGATATTGAACGGTATCTTTGTACTCGAACTTTTAACATGAGAAGTTcgag > 1:30181/1‑127 (MQ=255) ccaGCACCCCCCAAGTAGTTGCGGGGTGCTGGCGCGTCTTCATGCACGCTTGAGCCCCCGCACTTCTCACAGAATAAAAGATATTGAACGGTATCTTTGTACTCGAACTTTTAACATGAGAAGTTcgag < 2:30181/129‑3 (MQ=255) ccccccAAGTAGTTGCGGGGTGCTGGCGCGTCTTCATGCACGCTTGAGCCCCCGCACTTCTCACTGAATAAAAGATATTGAACGGTATCTTTGTACTCGAACTTTTCATGTTAAAAGTTCGCGGGATAACTATTTCCCTGTGTTTATGa < 7:143906/149‑1 (MQ=255) cccAAGTAGTTGCGGGGTGCTGGCGCGTCTTCATGCACGCTTGAGCCCCCGCACTTCTCACTGAATAAAAGATATTGAACGGTATCTTTGTACTCGAACTTTTCATGTTAAAAGTTCGCGGGATAACTATTTCCCTGTGTTTATGaaaa < 6:277561/149‑1 (MQ=255) ccAAGTAGTTGCGGGGTGCTGGCGCGTCTTCATGCACGCTTGAGCCCCCGCACTTCTCACTGAATAAAAGATATTGAACGGTATCTTTGTACTCGAACTTTTCATGTTAAAAGTTCGCGGGATAACTATTTCCCTGTGTTTATGAAAAg < 4:133738/149‑1 (MQ=255) aaGTAGTTGCGGGGTGCTGGCGCGTCTTCATGCACGCTTGAGCCCCCGCACTTCTCACTGAATAAAAGATATTGAACGGTATCTTTGTACTCGAACTTTTCATGTTAAAAGTTCGCGGGATAACTATTTCCCTGTGTTTATGAAAAgg > 7:81348/1‑148 (MQ=255) agtagtTGCGGGGTGCTGGCGCGTCTTCATGCACGCTTGAGCCCCCGCACTTCTCACTGAATAAAAGATATTGAACGGTATCTTTGTACTCGAACTTTTCATGTTAAAAGTTCGCGGGATAACTATTTCCCTGTGTTTATGAAAAGGaa < 3:93211/149‑1 (MQ=255) tagtTGCGGGGTGCTGGCGCGTCTTCATGCACGCTTGAGCCCCCGCACTTCTCACTGAATAAAAGATATTGAACGGTATCTTTGTACTCGAACTTTTAACATGAAAAGTTcga < 7:83960/113‑2 (MQ=255) tagtTGCGGGGTGCTGGCGCGTCTTCATGCACGCTTGAGCCCCCGCACTTCTCACTGAATAAAAGATATTGAACGGTATCTTTGTACTCGAACTTTTAACATGAAAAGTTcga > 8:83960/1‑112 (MQ=255) gcgcGTCTTCATGCACGCTTGAGCCCCCGCACTTCTCACTGAATAAAAGATATTGAACGGTATCTTTGTACTCGAACTTTTCATGTTAAAAGTTCGCGGGATAACTATTTCCCTGTGTTTATGAAAAGGAATCAAACCATGGATTgcgc < 5:83732/149‑1 (MQ=255) cATGCACGCTTGAGCCCCCGCACTTCTCACTGAATAAAAGATATTGAACGGTATCTTTGTACTCGAACTTTTCATGTTAAAAGTTCGCGGGATAACTATTTCCCTGTGTTTATGAAAAGGAATCAAACCATGGATTGCGCGAGTAGaaa > 2:202233/1‑149 (MQ=255) aTGCACGCTTGAGCCCCCGCACTTCTCACTGAATAAAAGATATTGAACGGTATCTTTGTACTCGAACTTTTCATGTTAAAAGTTCGCGGGATAACTATTTCCCTGTGTTTAt < 8:21287/112‑1 (MQ=255) aTGCACGCTTGAGCCCCCGCACTTCTCACTGAATAAAAGATATTGAACGGTATCTTTGTACTCGAACTTTTCATGTTAAAAGTTCGCGGGATAACTATTTCCCTGTGTTTAt > 7:21287/1‑112 (MQ=255) cTTGAG‑CCCCGCACTTCTCACTGAATAAAAGATATTGAACGGTATCTTTGTACTCGAACTTTTCATGTTAAAAGTTCGCGGGATAACTATTTCCCTGTGTTTATGAAAAGGAATCAAACCATGGATTGCGCGAGTAGAAAGTTCGCTGc < 5:319702/149‑1 (MQ=255) cccGCACTTCTCACTGAATAAAAGATATTGAACGGTATCTTTGTACTCGAACTTTTCATGTTAAAAGTTCGCGGGATAACTATTTCCCTGTGTTTATGAAAAGGAATCAAACCATGGATTGCGCGAGTAGAAAGTTCGCTGCCGAcaca < 4:259525/149‑1 (MQ=255) ccGCACTTCTCACTGAATAAAAGATATTGAACGGTATCTTTGTACTCGAACTTTTCATGTTAAAAGTTCGCGGGATAACTATTTCCCTGTGTTTATGAAAAGGAATCAAACCATGGATTGCGCGAGTAGAAAGTTCGCTGCCGACACaa > 1:217126/1‑149 (MQ=255) aCTTCTCACTGAATAAAAGATATTGAACGGTATCTTTGTACTCTAACTTTTCATGTTAAAAGTTCGCGGGATAACTATTTCCCTGTGTTTATGAAAAGGAATCAAACCATGGATTGCGCGAGTAGAAAGTTCGCTGCCGACACAACTTc < 5:20023/149‑1 (MQ=255) aCTGAATAAAAGATATTGAACGGTATCTTTGTACTCGAACTTTTCATGTTAAAAGTTCGCGGGATAACTATTTCCCTGTGTTTATGAAAAGGAATCAAACCATGGATTGCGCGa < 5:206261/114‑1 (MQ=255) aCTGAATAAAAGATATTGAACGGTATCTTTGTACTCGAACTTTTCATGTTAAAAGTTCGCGGGATAACTATTTCCCTGTGTTTATGAAAAGGAATCAAACCATGGATTGCGCGa > 6:206261/1‑114 (MQ=255) cTGAATAAAAGATATTGAACGGTATCTTTGTTCTCGAACTTTTCATGTTAAAAGTTCGCGGGATAACTATTTCCCTGTGTTTATGAAAAGGAATCAAACCATGGATTGCGCGAGTAGAAAGTTCGCTGCCGACACAACTTCCGACtctc > 8:236179/1‑149 (MQ=255) tGAATAAAAGATATTGAACGGTATCTTTGTACTCGAACTTTTCATGTTAAAAGTTCGCGGGATAACTATTTCCCTGTGTTTATGAAAAGGAATCAAACCATGGATTGCGCGAGTAGAAAGTTCGCTGCCGACACAACTTCCGACTCTCg > 5:310416/1‑149 (MQ=255) aaTAAAAGATATTGAACGGTATCTTTGTACTCGAACTTTTCATGTTAAAAGTTCGCGGGATAACTATTTCCCTGTGTTTATGAAAAGGAATCAAACCATGGATTGCGCGAGTAGAAAGTTCGCTGCCGACACAACTTCCGACTCTCgg > 8:48133/1‑148 (MQ=255) aaaaGATATTGAACGGTATCTTTGTACTCGAACTTTTCATGTTAAAAGTTCGCGGGATAACTATTTCCCTGTGTTTATGAAAAGGAATCAAACCATGGATTGCGAGAGTAGAAAGTTCGCTGCCTACACAACTTCCGACTCTCTGCAGc > 4:304201/1‑149 (MQ=255) aaaGATATTGAACGGTATCTTTGTACTCGAACTTTTCATGTT‑AAAGTTCGCGGGATAACTATTTCCCTGTGTTTATGAAAAGGAATCAAACCATGGATTGCGCGAGTAGAAAGTTCGCTGCCGACACAACTTCCGACTCTCGGCAGCg > 7:80020/1‑148 (MQ=255) aaGATATTGAACGGTATCTTTGTACTCGAACTTTTCATGTTAAAAGTTCGCGGGATAACTATTTCCCTGTGTTTATGAAAAGGAATCAAACCATGGATTGCGCGAGTAGAAAGTTCGCTGCCGACACAACTTCCGACTCTCGGCAGCg > 4:129375/1‑148 (MQ=255) tatTGAACGGTATCTTTGTACTCGAACTTTTCATGTTAAAAGTTCGCGGGATAACTATTTCCCTGTGTTTATGAAAAGGAATCAAACCATGGATTGCGCGAGTAGAAAGTTCGCTGCCGACACAACTTCCGACTCTCGGCAGCGgaca < 6:310416/148‑1 (MQ=255) ttGAACGGTATCTTTGTACTCGAACTTTTCATGTT‑AAAGTTCGCGGGATAACTATTTCCCTGTGTTTATGAAAAGGAATCAAACCATGGATTGCGCGAGTAGAAAGTTCGCTGCCGACACAACTTCCGACTCTCGGCAGCGgacagac < 8:80020/148‑1 (MQ=255) gAACGGTATCTTTGTACTCGAACTTTTCATGTTAAAAGTTCGCGGGATAACTATTTCCCTGTGTTTATGAAAAGGAATCAAACCATGGATTGCGCGAGTAGAAAGTTCGCTGCCGACACAACTTCCGACTCTCGGCAGCGgacagaca > 2:12115/1‑148 (MQ=255) ggTATCTTTGTACTCGAACTTTTCATGTTAAAAGTTCGCGGGATAACTATTTCCCTGTGTTTATGAAAAGGAATCAAACCATGGATTGCGCGAGTAGAAAGTTCGCTGCCGACACAACTTCCGACTCTCGGCAGCGGACAGACAACTGt > 4:166646/1‑149 (MQ=255) tATCTTTGTACTCGAACTTTTCATGTTAAAAGTTCGCGGGATAACTATTTCCCTGTGTTTATGAAAAGGAATCAAACCATGGATTGCGCGAGTAGAAAGTTCGCTGCCGACACAACTTCCGACTCTCGGCAGCGGACAGACAACTGTCg > 6:264691/1‑149 (MQ=255) tGTACTCGAACTTTTCATGTTAAAAGTTCGCGGGATAACTATTTCCCTGTGTTTATGAAAAGGAATCAAACCATGGATTGCGCGAGTAGAAAGTTCGCTGCCGACACAACTTCCGACTCTCGGCAGCGGACAGACAACTGTCGGaaa < 3:166646/147‑1 (MQ=255) tCGAACTTTTCATGTTAAAAGTTCGCGGGATAACTATTTCCCTGTGTTTATGAAAAGGAATCAAACCATGGATTGCGCGAGTAGAAAGTTCGCTGCCGACACAACTTCCGACTCTCGGCAGCGGACAGACAACTGTCGGAAAGAACCAg > 1:257604/1‑149 (MQ=255) ttttCATGTTAAAAGTTCGCGGGATAACTATTTCCCTGTGTTTATGAAAAGGAATCAAACCATGGATTGCGCGAGTAGAAAGTTCGCTGCCGACACAACTTCCGACTCTCGGCAGCGGACAGACAACTGTCGGAAAGAACCAGCattat > 8:248828/1‑149 (MQ=255) ttCATGTTAAAAGTTCGCGGGATAACTATTTCCCTGTGTTTATGAAAAGGAATCAAACCATGGATTGCGCGAGTAGAAAGTTCGCTGCCGACACAACTTCCGACTCTCGGCAGCGGACAGACAACTGTCGGAAAGAACCAGCattatt < 8:81348/148‑1 (MQ=255) cATGTTAAAAGTTCGCGGGATAACTATTTCCCTGTGTTTATGAAAAGGAATCAAACCATGGATTGCGCGAGTAGAAAGTTCGCTGCCGACACAACTTCCGACTCTCGGCAGCGGACAGACAACTGTCGG‑AAGAACCAGCATTATTCAg < 2:217126/148‑1 (MQ=255) | GCGTCTGCACGGCTACCTTGACCACCACCAGCACCCCCCAAGTAGTTGCGGGGTGCTGGCGCGTCTTCATGCACGCTTGAGCCCCCGCACTTCTCACTGAATAAAAGATATTGAACGGTATCTTTGTACTCGAACTTTTCATGTTAAAAGTTCGCGGGATAACTATTTCCCTGTGTTTATGAAAAGGAATCAAACCATGGATTGCGCGAGTAGAAAGTTCGCTGCCGACACAACTTCCGACTCTCGGCAGCGGACAGACAACTGTCGGAAAGAACCAGCATTATTCAG > NC_002947/3030376‑3030663 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |