Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	3,030,520	T→G	8.2%	intergenic (+520/‑174)	pcaY → / → mgtA	aromatic acid chemoreceptor/ATP‑dependent magnesium transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	3,030,520	0	T	G	8.2%	146.6 / 4.7	49	intergenic (+520/‑174)	pcaY/mgtA	aromatic acid chemoreceptor/ATP‑dependent magnesium transporter
Reads supporting (aligned to +/- strand):  ref base T (25/20);  new base G (2/2);  total (27/22)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.44e-01

GCGTCTGCACGGCTACCTTGACCACCACCAGCACCCCCCAAGTAGTTGCGGGGTGCTGGCGCGTCTTCATGCACGCTTGAGCCCCCGCACTTCTCACTGAATAAAAGATATTGAACGGTATCTTTGTACTCGAACTTTTCATGTTAAAAGTTCGCGGGATAACTATTTCCCTGTGTTTATGAAAAGGAATCAAACCATGGATTGCGCGAGTAGAAAGTTCGCTGCCGACACAACTTCCGACTCTCGGCAGCGGACAGACAACTGTCGGAAAGAACCAGCATTATTCAGCGA  >  NC_002947/3030376‑3030666                                                                                                                                                 |                                                                                                                                                   gcgTCTGCACGGCTACCTTGACCACCACCAGCACCCCCCAAGTAGTTGCGGGGTGCTGGCGCGTCTTCATGCACGCTTGAGCCCCCGCACTTCTCACTGAATAAAAGATATTGAACGGTATCTTTGTACTCGAACTTTTCATGTTaaaa                                                                                                                                                <  5:267611/149‑1 (MQ=255)   gTCTGCACGGCTACCTTGACCACCACCAGCACCCCCCAAGTAGTTGCGGGGTGCTGGCGCGTCTTCATGCACGCTTGAGCCCCCGCACTTCTCACTGAATAAAAGATATTGAACGGTATCTTTGTACTCGAACTTTTCATGTTAAAAGt                                                                                                                                              >  2:297466/1‑149 (MQ=255)    tCTGCACGGCTACCTTGACCACCACCAGCACCCCCCAAGTAGTTGCGGGGTGCTGGCGCGTCTTCATGCACGCTTGAGCCCCCGCACTTCTCACTGAATAAAAGATATTGAACGGTATCTTTGTACTCGAACTTTTCATGTTAAAAGtt                                                                                                                                             >  5:277561/1‑149 (MQ=255)       gCACGGCTACCTTGACCACCACCAGCACCCCCCAAGTAGTTGCGGGGTGCTGGCGCGTCTTCATGCACGCTTGAGCCCCCGCACTTCTCACTGAATAAAAGATATTGAACGGTATCTTTGTACTCGAACTTTTCATGTTAAAAGTTcgc                                                                                                                                          >  4:93211/1‑149 (MQ=255)        cACGGCTACCTTGACCACCACCAGCACCCCCCAAGTAGTTGCGGGGTGCTGGCGCGTCTTCATGCACGCTTGAGCCCCCGCACTTCTCACTGAATAAAAGATATTGAACGGTATCTTTGTACTCGAACTTTTCATGTTAAAAGTTcgcg                                                                                                                                         <  1:124525/149‑1 (MQ=255)        cACGGCTACCTTGACCACCACCAGCACCCCCCAAGTAGTTGCGGGGTGCTGGCGCGTCTTCATGCACGCTTGAGCCCCCGCACTTCTCACTGAATAAAAGATATTGAACGGTATCTTTGTACTCGAACTTTTCATGTTAAAAGTTcgcg                                                                                                                                         >  8:143906/1‑149 (MQ=255)                      ccaccaccaGCACCCCCCAAGTAGTTGCGGGGTGCTGGCGCGTCTTCATGCACGCTTGAGCCCCCGCACTTCTCACTGAATAAAAGATATTGAACGGTATCTTTGTACTCGAACTTTTCATGTTAAAAGTTCGCGGGATAACTATTTcc                                                                                                                           <  6:221975/149‑1 (MQ=255)                      ccaccaccaGCACCCCCCAAGTAGTTGCGGGGTGCTGGCGCGTCTTCATGCACGCTTGAGCCCCCGCACTTCTCACTGAATAAAAGATATTGAACGGTATCTTTGTACTCGAACTTTTCATGTTAAAAGTTCGCGGGATAACTATTTcc                                                                                                                           <  1:297466/149‑1 (MQ=255)                        accaccaGCACCCCCCAAGTAGTTGCGGGGTGCTGGCGAGTCTTCATGCACTCATGAGCCCCCGCACTTCTCACTGAATAAAAGATATTGAACGGTATCTTTGTACTCGAACTTTTCATGTTAAAAGTTCGCGGGATAACTATTTCCCt                                                                                                                         >  6:20023/1‑149 (MQ=255)                         gcgccaGCACCCCCCAAGTAGTTGCGGGGTGCTGGCGCGTCTTCATGCACGCTTGAGCCCCCGCACTTCTCACTGAATAAAAGATATTGAACGGTATCTTTGTACTCGAACTTTTCATGTTAAAAGTTCGCGGGATAACTATTTCCCtg                                                                                                                        >  6:83732/4‑149 (MQ=255)                          caccaGCACCCCCCAAGTAGTTGCGGGGTGCTGGCGCGTCTTCATGCACGCTTGAGCCCCCGCACTTCTCACTGAATAAAAGATATTGAACGGTATCTTTGTACTCGAACTTTTCATGTTAAAAGTTCGCGGGATAACTATTTCCCtgt                                                                                                                       >  2:287159/1‑149 (MQ=255)                          caccaGCACCCCCCAAGTAGTTGCGGGGTGCTGGCGCGTCTTCATGCACGCTTGAGCCCCCGCACTTCTCACTGAATAAAAGATATTGAACGGTATCTTTGTACTCGAACTTTTCATGTTAAAAGTTCGCGGGATAACTATTTCCCtgt                                                                                                                       <  2:287944/149‑1 (MQ=255)                           gccaGCACCCCGCAACTACTTGGGGGGTGCTGGTGCGTCTTCATGCACGCTTGAGCCCCCGCACTTCTCACTGAATAAAAGATATTGAACGGTATCTTTGTACTCGAACTTTTCATGTTAAAAGTTCGCGGGATAACTATTTCCCtgtg                                                                                                                      >  4:116899/2‑149 (MQ=39)                           gccaGCACCCCGCAACTACTTGGGGGGTGCTGGCGCGTCTTCATGCACGCTTGCG‑CCCCGCACTTCTCACTGAATAAAAGATATTGAACGGTATCTTTGTACTCGAACTTTTCATGTTAAAAGTTCGCGGGATAACTATTTCCCTgtgt                                                                                                                     >  6:319702/2‑149 (MQ=255)                            ccaGCACCCCCCAAGTAGTTGCGGGGTGCTGGCGCGTCTTCATGCACGCTTGAGCCCCCGCACTTCTCACAGAATAAAAGATATTGAACGGTATCTTTGTACTCGAACTTTTAACATGAGAAGTTcgag                                                                                                                                         <  2:30181/129‑3 (MQ=255)                            ccaGCACCCCCCAAGTAGTTGCGGGGTGCTGGCGCGTCTTCATGCACGCTTGAGCCCCCGCACTTCTCACAGAATAAAAGATATTGAACGGTATCTTTGTACTCGAACTTTTAACATGAGAAGTTcgag                                                                                                                                         >  1:30181/1‑127 (MQ=255)                                  ccccccAAGTAGTTGCGGGGTGCTGGCGCGTCTTCATGCACGCTTGAGCCCCCGCACTTCTCACTGAATAAAAGATATTGAACGGTATCTTTGTACTCGAACTTTTCATGTTAAAAGTTCGCGGGATAACTATTTCCCTGTGTTTATGa                                                                                                               <  7:143906/149‑1 (MQ=255)                                     cccAAGTAGTTGCGGGGTGCTGGCGCGTCTTCATGCACGCTTGAGCCCCCGCACTTCTCACTGAATAAAAGATATTGAACGGTATCTTTGTACTCGAACTTTTCATGTTAAAAGTTCGCGGGATAACTATTTCCCTGTGTTTATGaaaa                                                                                                            <  6:277561/149‑1 (MQ=255)                                      ccAAGTAGTTGCGGGGTGCTGGCGCGTCTTCATGCACGCTTGAGCCCCCGCACTTCTCACTGAATAAAAGATATTGAACGGTATCTTTGTACTCGAACTTTTCATGTTAAAAGTTCGCGGGATAACTATTTCCCTGTGTTTATGAAAAg                                                                                                           <  4:133738/149‑1 (MQ=255)                                        aaGTAGTTGCGGGGTGCTGGCGCGTCTTCATGCACGCTTGAGCCCCCGCACTTCTCACTGAATAAAAGATATTGAACGGTATCTTTGTACTCGAACTTTTCATGTTAAAAGTTCGCGGGATAACTATTTCCCTGTGTTTATGAAAAgg                                                                                                          >  7:81348/1‑148 (MQ=255)                                         agtagtTGCGGGGTGCTGGCGCGTCTTCATGCACGCTTGAGCCCCCGCACTTCTCACTGAATAAAAGATATTGAACGGTATCTTTGTACTCGAACTTTTCATGTTAAAAGTTCGCGGGATAACTATTTCCCTGTGTTTATGAAAAGGaa                                                                                                        <  3:93211/149‑1 (MQ=255)                                           tagtTGCGGGGTGCTGGCGCGTCTTCATGCACGCTTGAGCCCCCGCACTTCTCACTGAATAAAAGATATTGAACGGTATCTTTGTACTCGAACTTTTAACATGAAAAGTTcga                                                                                                                                          <  7:83960/113‑2 (MQ=255)                                           tagtTGCGGGGTGCTGGCGCGTCTTCATGCACGCTTGAGCCCCCGCACTTCTCACTGAATAAAAGATATTGAACGGTATCTTTGTACTCGAACTTTTAACATGAAAAGTTcga                                                                                                                                          >  8:83960/1‑112 (MQ=255)                                                           gcgcGTCTTCATGCACGCTTGAGCCCCCGCACTTCTCACTGAATAAAAGATATTGAACGGTATCTTTGTACTCGAACTTTTCATGTTAAAAGTTCGCGGGATAACTATTTCCCTGTGTTTATGAAAAGGAATCAAACCATGGATTgcgc                                                                                      <  5:83732/149‑1 (MQ=255)                                                                    cATGCACGCTTGAGCCCCCGCACTTCTCACTGAATAAAAGATATTGAACGGTATCTTTGTACTCGAACTTTTCATGTTAAAAGTTCGCGGGATAACTATTTCCCTGTGTTTATGAAAAGGAATCAAACCATGGATTGCGCGAGTAGaaa                                                                             >  2:202233/1‑149 (MQ=255)                                                                     aTGCACGCTTGAGCCCCCGCACTTCTCACTGAATAAAAGATATTGAACGGTATCTTTGTACTCGAACTTTTCATGTTAAAAGTTCGCGGGATAACTATTTCCCTGTGTTTAt                                                                                                                 >  7:21287/1‑112 (MQ=255)                                                                     aTGCACGCTTGAGCCCCCGCACTTCTCACTGAATAAAAGATATTGAACGGTATCTTTGTACTCGAACTTTTCATGTTAAAAGTTCGCGGGATAACTATTTCCCTGTGTTTAt                                                                                                                 <  8:21287/112‑1 (MQ=255)                                                                            cTTGAG‑CCCCGCACTTCTCACTGAATAAAAGATATTGAACGGTATCTTTGTACTCGAACTTTTCATGTTAAAAGTTCGCGGGATAACTATTTCCCTGTGTTTATGAAAAGGAATCAAACCATGGATTGCGCGAGTAGAAAGTTCGCTGc                                                                    <  5:319702/149‑1 (MQ=255)                                                                                    cccGCACTTCTCACTGAATAAAAGATATTGAACGGTATCTTTGTACTCGAACTTTTCATGTTAAAAGTTCGCGGGATAACTATTTCCCTGTGTTTATGAAAAGGAATCAAACCATGGATTGCGCGAGTAGAAAGTTCGCTGCCGAcaca                                                             <  4:259525/149‑1 (MQ=255)                                                                                     ccGCACTTCTCACTGAATAAAAGATATTGAACGGTATCTTTGTACTCGAACTTTTCATGTTAAAAGTTCGCGGGATAACTATTTCCCTGTGTTTATGAAAAGGAATCAAACCATGGATTGCGCGAGTAGAAAGTTCGCTGCCGACACaa                                                            >  1:217126/1‑149 (MQ=255)                                                                                         aCTTCTCACTGAATAAAAGATATTGAACGGTATCTTTGTACTCTAACTTTTCATGTTAAAAGTTCGCGGGATAACTATTTCCCTGTGTTTATGAAAAGGAATCAAACCATGGATTGCGCGAGTAGAAAGTTCGCTGCCGACACAACTTc                                                        <  5:20023/149‑1 (MQ=255)                                                                                                aCTGAATAAAAGATATTGAACGGTATCTTTGTACTCGAACTTTTCATGTTAAAAGTTCGCGGGATAACTATTTCCCTGTGTTTATGAAAAGGAATCAAACCATGGATTGCGCGa                                                                                    >  6:206261/1‑114 (MQ=255)                                                                                                aCTGAATAAAAGATATTGAACGGTATCTTTGTACTCGAACTTTTCATGTTAAAAGTTCGCGGGATAACTATTTCCCTGTGTTTATGAAAAGGAATCAAACCATGGATTGCGCGa                                                                                    <  5:206261/114‑1 (MQ=255)                                                                                                 cTGAATAAAAGATATTGAACGGTATCTTTGTTCTCGAACTTTTCATGTTAAAAGTTCGCGGGATAACTATTTCCCTGTGTTTATGAAAAGGAATCAAACCATGGATTGCGCGAGTAGAAAGTTCGCTGCCGACACAACTTCCGACtctc                                                >  8:236179/1‑149 (MQ=255)                                                                                                  tGAATAAAAGATATTGAACGGTATCTTTGTACTCGAACTTTTCATGTTAAAAGTTCGCGGGATAACTATTTCCCTGTGTTTATGAAAAGGAATCAAACCATGGATTGCGCGAGTAGAAAGTTCGCTGCCGACACAACTTCCGACTCTCg                                               >  5:310416/1‑149 (MQ=255)                                                                                                    aaTAAAAGATATTGAACGGTATCTTTGTACTCGAACTTTTCATGTTAAAAGTTCGCGGGATAACTATTTCCCTGTGTTTATGAAAAGGAATCAAACCATGGATTGCGCGAGTAGAAAGTTCGCTGCCGACACAACTTCCGACTCTCgg                                              >  8:48133/1‑148 (MQ=255)                                                                                                       aaaaGATATTGAACGGTATCTTTGTACTCGAACTTTTCATGTTAAAAGTTCGCGGGATAACTATTTCCCTGTGTTTATGAAAAGGAATCAAACCATGGATTGCGAGAGTAGAAAGTTCGCTGCCTACACAACTTCCGACTCTCTGCAGc                                          >  4:304201/1‑149 (MQ=255)                                                                                                        aaaGATATTGAACGGTATCTTTGTACTCGAACTTTTCATGTT‑AAAGTTCGCGGGATAACTATTTCCCTGTGTTTATGAAAAGGAATCAAACCATGGATTGCGCGAGTAGAAAGTTCGCTGCCGACACAACTTCCGACTCTCGGCAGCg                                         >  7:80020/1‑148 (MQ=255)                                                                                                         aaGATATTGAACGGTATCTTTGTACTCGAACTTTTCATGTTAAAAGTTCGCGGGATAACTATTTCCCTGTGTTTATGAAAAGGAATCAAACCATGGATTGCGCGAGTAGAAAGTTCGCTGCCGACACAACTTCCGACTCTCGGCAGCg                                         >  4:129375/1‑148 (MQ=255)                                                                                                             tatTGAACGGTATCTTTGTACTCGAACTTTTCATGTTAAAAGTTCGCGGGATAACTATTTCCCTGTGTTTATGAAAAGGAATCAAACCATGGATTGCGCGAGTAGAAAGTTCGCTGCCGACACAACTTCCGACTCTCGGCAGCGgaca                                     <  6:310416/148‑1 (MQ=255)                                                                                                               ttGAACGGTATCTTTGTACTCGAACTTTTCATGTT‑AAAGTTCGCGGGATAACTATTTCCCTGTGTTTATGAAAAGGAATCAAACCATGGATTGCGCGAGTAGAAAGTTCGCTGCCGACACAACTTCCGACTCTCGGCAGCGgacagac                                  <  8:80020/148‑1 (MQ=255)                                                                                                                 gAACGGTATCTTTGTACTCGAACTTTTCATGTTAAAAGTTCGCGGGATAACTATTTCCCTGTGTTTATGAAAAGGAATCAAACCATGGATTGCGCGAGTAGAAAGTTCGCTGCCGACACAACTTCCGACTCTCGGCAGCGgacagaca                                 >  2:12115/1‑148 (MQ=255)                                                                                                                     ggTATCTTTGTACTCGAACTTTTCATGTTAAAAGTTCGCGGGATAACTATTTCCCTGTGTTTATGAAAAGGAATCAAACCATGGATTGCGCGAGTAGAAAGTTCGCTGCCGACACAACTTCCGACTCTCGGCAGCGGACAGACAACTGt                            >  4:166646/1‑149 (MQ=255)                                                                                                                       tATCTTTGTACTCGAACTTTTCATGTTAAAAGTTCGCGGGATAACTATTTCCCTGTGTTTATGAAAAGGAATCAAACCATGGATTGCGCGAGTAGAAAGTTCGCTGCCGACACAACTTCCGACTCTCGGCAGCGGACAGACAACTGTCg                          >  6:264691/1‑149 (MQ=255)                                                                                                                             tGTACTCGAACTTTTCATGTTAAAAGTTCGCGGGATAACTATTTCCCTGTGTTTATGAAAAGGAATCAAACCATGGATTGCGCGAGTAGAAAGTTCGCTGCCGACACAACTTCCGACTCTCGGCAGCGGACAGACAACTGTCGGaaa                      <  3:166646/147‑1 (MQ=255)                                                                                                                                  tCGAACTTTTCATGTTAAAAGTTCGCGGGATAACTATTTCCCTGTGTTTATGAAAAGGAATCAAACCATGGATTGCGCGAGTAGAAAGTTCGCTGCCGACACAACTTCCGACTCTCGGCAGCGGACAGACAACTGTCGGAAAGAACCAg               >  1:257604/1‑149 (MQ=255)                                                                                                                                        ttttCATGTTAAAAGTTCGCGGGATAACTATTTCCCTGTGTTTATGAAAAGGAATCAAACCATGGATTGCGCGAGTAGAAAGTTCGCTGCCGACACAACTTCCGACTCTCGGCAGCGGACAGACAACTGTCGGAAAGAACCAGCattat         >  8:248828/1‑149 (MQ=255)                                                                                                                                          ttCATGTTAAAAGTTCGCGGGATAACTATTTCCCTGTGTTTATGAAAAGGAATCAAACCATGGATTGCGCGAGTAGAAAGTTCGCTGCCGACACAACTTCCGACTCTCGGCAGCGGACAGACAACTGTCGGAAAGAACCAGCattatt        <  8:81348/148‑1 (MQ=255)                                                                                                                                            cATGTTAAAAGTTCGCGGGATAACTATTTCCCTGTGTTTATGAAAAGGAATCAAACCATGGATTGCGCGAGTAGAAAGTTCGCTGCCGACACAACTTCCGACTCTCGGCAGCGGACAGACAACTGTCGG‑AAGAACCAGCATTATTCAg     <  2:217126/148‑1 (MQ=255)                                                                                                                                                ttAAAAGTTAGCGGGATAACTATTTCCCTGTGTTTATGAAAAGGAATCAAACCATGGATTGCGCGAGTAGAAAGTTCGCTGCCGACACAACTTCCGACTCTCGCCAGCGGACAGACAACTGTCGGAAAGAAACAGCATTATTCAGCGa  >  5:64394/1‑148 (MQ=255)                                                                                                                                                 |                                                                                                                                                   GCGTCTGCACGGCTACCTTGACCACCACCAGCACCCCCCAAGTAGTTGCGGGGTGCTGGCGCGTCTTCATGCACGCTTGAGCCCCCGCACTTCTCACTGAATAAAAGATATTGAACGGTATCTTTGTACTCGAACTTTTCATGTTAAAAGTTCGCGGGATAACTATTTCCCTGTGTTTATGAAAAGGAATCAAACCATGGATTGCGCGAGTAGAAAGTTCGCTGCCGACACAACTTCCGACTCTCGGCAGCGGACAGACAACTGTCGGAAAGAACCAGCATTATTCAGCGA  >  NC_002947/3030376‑3030666

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑