Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,057,908 T→C 7.4% S222G (AGC→GGC)  PP_5538 ← ABC transporter substrate‑binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,057,9080TC 7.4% 152.1 / 2.5 54S222G (AGC→GGC) PP_5538ABC transporter substrate‑binding protein
Reads supporting (aligned to +/- strand):  ref base T (24/26);  new base C (2/2);  total (26/28)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.27e-01

GTGGGGGTCAGGCCCTGGGTACCGGCCACCGGACGTGGATACCACGTCTGGTAGGGCAGGTACTCGCCGAAGTCACCGCGTTCGTCGGCCACCAGTACCACGTCGTACTCGGCAGTCTGGGTGAACAGCGGCATGTCGGCCTGGGCGCTGCGGCGCTGGTCGTTGTCGAAGGTCCAGGTTTTTTCCGCGACCAGTTGCACGCCAAAGCGCTTCACCGACCGCCGCAGGGCAGCGGCGTACGCCTGATCGTCCGGGGTCTGGCCGACCACCAGCAATGCCCGTTGCCATTTGC  >  NC_002947/3057760‑3058051
                                                                                                                                                    |                                                                                                                                               
gTGGGGGTCAGGCCCTGGGTACCGGCCACCGGACGTGGATACCACGTCTGGTAGGGCAGGTACTCGCCGAAGTCACCGCGTTCGTCGGCCACCAGTACCACGTCGTACTCGGCAGTCTGGGTGAACAGCGGCATGTCGGCCTGGGCGCt                                                                                                                                                 >  3:309626/1‑149 (MQ=255)
        cAGGCCCTGGGTACCGGCCACCGGACGTGGATACCACGTCTGGTAGGGCAGGTACTCGCCGAAGTCACCGCGTTCGTCGGCCACCAGTACCACGTCGTACTCGGCAGTCTGGGTGAACAGCGGCATGTCGGCCTGGGCGCTGCGGCGCt                                                                                                                                         <  8:70043/149‑1 (MQ=255)
                gggTACCGGCCACCGGACGTGGATACCACGTCTGGTAGGGCAGGTACTCGCCGAAGTCACCGCGTTCGTCGGCCACCAGTACCACGTCGTACTCGGCAGTCT‑GGTGAACAGCGGCATGTCGGCCTGGGCGCTGCGGCGCTGGTCGTTGt                                                                                                                                <  4:223418/149‑1 (MQ=255)
                 ggTACCGGCCACCGGACGTGGATACCACGTCTGGTAGGGCAGGTACTCGCCGAAGTCACCGCGTTCGTCGGCCACCAGTACCACGTCGTACTCGGCAGTCTGGGTGAACAGCGGCATGTCGGCCTGGGCGCTGCGGCGCTGGTCGtt                                                                                                                                  <  8:189420/147‑1 (MQ=255)
                 ggTACCGGCCACCGGACGTGGATACCACGTCTGGTAGGGCAGGTACTCGCCGAAGTCACCGCGTTCGTCGGCCACCAGTACCACGTCGTACTCGGCAGTCTGGGTGAACAGCGGCATGTCGGCCTGGGCGCTGCGGCGCTGGTCGTTGt                                                                                                                                <  5:90231/149‑1 (MQ=255)
                 ggTACCGGCCACCGGACGTGGATACCACGTCTGGTAGGGCAGGTACTCGCCGAAGTCACCGCGTTCGTCGGCCACCAGTACCACGTCGTACTCGGCAGTCTGGGTGAACAGCGGCATGTCGGCCTGGGCGCTGCGGCGCTGGTCGTTGt                                                                                                                                <  7:35867/149‑1 (MQ=255)
                     ccGGCCACCGGACGTGGATACCACGTCTGGTAGGGCAGGTACTCGCCGAAGTCACCGCGTTCGTCGGCCACCAGTACCACGTCGTACTCGGCAGTCTGGGTGAACAGCGGCATGTCGGCCTGGGCGCTGCGGCGCTGGTCGTTGTCGaa                                                                                                                            <  6:40384/149‑1 (MQ=255)
                       ggCCACCGGACGTGGATACCACGTCTGGTAGGGCAGGTACTCGCCGAAGTCACCGCGTTCGTCGGCCACCAGTACCACGTCGTACTCGGCAGTCTGGGTGAACAGCGGCATGTCGGCCTGGGCGCTGCGGCGCTGGTCGTTGTCGAAgg                                                                                                                          >  6:29587/1‑149 (MQ=255)
                        gCCACCGGACGTGGATACCACGTCTGGTAGGGCAGGTACTCGCCGAAGTCACCGCGTTCGTCGGCCACCAGTACCACGTCGTACTCGGCAGTCTGGGTGAACAGCGGCATGTCGGCCTGGGCGCTGCGGCGCTGGTCGTTGTCGAAGGt                                                                                                                         >  8:147056/1‑149 (MQ=255)
                             cGGACGTGGATACCACGTCTGGTAGGGCAGGTACTCGCCGAAGTCACCGCGTTCGTCGGCCACCAGTACCACGTCGTACTCGGCAGTCTGGGTGAACAGCGGCATGTCGGCCTGGGCGCTGCGGCGCTGGTCGTTGTCGAAGGTCCAgg                                                                                                                    >  4:102730/1‑149 (MQ=255)
                               gACGTGGATACCACGTCTGGTAGGGCAGGTACTCGCCGAAGTCACCGCGTTCGTCGGCCACCAGTACCACGTCGTACTCGGCAGTCTGGGTGAACAGCGGCATGTCGGCCTGGGCGCTGCGGCGCTGGTCGTTGTCGAAGGTCCAGGtt                                                                                                                  >  4:107678/1‑149 (MQ=255)
                                       tACCACGTCTGGTAGGGCAGGTACTCGCCGAAGTCACCGCGTTCGTCGGCCACCAGTACCACGTCGTACTCGGCAGTCTGGGTGAACAGCGGCATGTCGGCCTGGGCGCTGCGGCGCTGGTCGTTGTCGAAGGTCCAGGTTTTTTCcgc                                                                                                          <  1:126192/149‑1 (MQ=255)
                                       tACCACGTCTGGTAGGGCAGGTACTCGCCGAAGTCACCGCGTTCGTCGGCCACCAGTACCACGTCGTACTCGGCAGTCTGGGTGAACAGCGGCATGTCGGCCTGGGCGCTGCGGCGCTGGTCGTTGTCGAAGGTCCAGGTTTTTTCcgc                                                                                                          >  5:341569/1‑149 (MQ=255)
                                          cACGTCTGGTAGGGCAGGTACTCGCCGAAGTCACCGCGTTCGTCGGCCACCAGTACCACGTCGTACTCGGCAGTCTGGGTGAACAGCGGCATGTCGGCCTGGGCGCTGCGGCGCTGGTCGTTGTCGAAGGTCCAGGTTTTTTCCGCGAc                                                                                                       >  7:314763/1‑149 (MQ=255)
                                          cACGTCTGGTAGGGCAGGTACTCGCCGAAGTCACCGCGTTCGTCGGCCACCAGTACCACGTCGTACTCGGCAGTCTGGGTGAACAGCGGCATGTCGGCCTGGGCGCTGCGGCGCTGGTCGTTGTCGAAGGTCCAGGTTTTTTCCGCGAc                                                                                                       >  7:287512/1‑149 (MQ=255)
                                           aCGTCTGGTAGGGCAGGTACTCGCCGAAGTCACCGCGTTCGTCGGCCACCAGTACCACGTCGTACTCGGCAGTCTGGGTGAACAGCGGCATGTCGGCCTGGGCGCTGCGGCGCTGGTCGTTGTCGAAGGTCCAGGTTTTTTCCGCGAc                                                                                                       >  2:293167/1‑148 (MQ=255)
                                                  gTAGGGCAGGTACTCGCCGAAGTCACCGCGTTCGTCGGCCACCAGTACCACGTCGTACTCGGCAGTCTGGGTGAACAGCGGCATGTCGGCCTGGGCGCTGCGGCGCTGGTCGTTGTCGAAGGTCCAGGTTTTTTCCGCGACCAGtt                                                                                                  <  6:221396/146‑1 (MQ=255)
                                                     gggCAGGTACTCGCCGAAGTCACCGCGTTCGTCGGCCACCAGTACCACGTCGTACTCGGCAGTCTGGGTGAACAGCGGCATGTCGGCCTGGGCGCTGCGGCGCTGGTCGTTGTCGAAGGTCCAGGTTTTTTCCGCGACCAGTTGCACGc                                                                                            >  6:109985/1‑149 (MQ=255)
                                                          ggTACTCGCCGAAGTCACCGCGTTCGTCGGCCACCAGTACCACGTCGTACTCGGCAGTCTGGGTGAACAGCGGCATGTCGGCCTGGGCGCTGCGGCGCTGGTCGTTGTCGAAGGTCCAGGTTTTTTCCGCGACCAGTTGCACGCCAAAg                                                                                       >  4:139696/1‑149 (MQ=255)
                                                              cTCGCCGAAGTCACCGCGTTCGTCGGCCACCATTACCACGTCGTACTCGGCAGTCTGGGTGAACAGCGGCATGTCGGCCTGGGCGCTGCGGCGCTGGTCGTTGTCGAAGGTCCAGGTTTTTTCCGCGACCAGTTGCACGCCAAAGCGCt                                                                                   >  1:212363/1‑149 (MQ=255)
                                                               tCGCCGAAGTCACCGCGTTCGTCGGCCACCAGTACCACGTCGTACTCGGCAGTCTGGGTGAACAGCGGCATGTCGGCCTGGGCGCTGCGGCGCTGGTCGTTGTCGAAGGTCCAGGTTTTTTCCGCGACCAGTTGCACGCCAAAGCGCtt                                                                                  >  1:208558/1‑149 (MQ=255)
                                                                 gCCGAAGTCACCGCGTTCGTCGGCCACCAGTACCACGTCGTACTCGGCAGTCTGGGTGAACAGCGGCATGTCGGCCTGGGCGCTGCGGCGCTGGTCGTTGTCGAAGGTCCAGGTTTTTTCCGCGACCAGTTGCACGCCAAAGCGCTTCa                                                                                >  7:203292/1‑149 (MQ=255)
                                                                  ccGAAGTCACCGCGTTCGTCGGCCACCAGTACCACGTCCTACTCGGCAGTCTCGGTGAACAGCGGCATGTCGGCCTGGGCGCTGCGGCGCTGGTCGTTGTCGAAGGTCCAGGTTTTTTCCGCGACCAGTTGCACGCCAAAGAGCTTCac                                                                               >  1:48112/1‑149 (MQ=255)
                                                                          aCCGCGTTCGTCGGCCACCAGTACCACGTCGTACTCGGCAGTCTGGGTGAACAGCGGCATGTCGGCCTGGGCGCTGCGGCGCTGTTCGTTGTCGAAGGTCCAGGTTTTTTCCGCGACCAGTTGCACGCCAAAGCGCTTCACCGAccgc                                                                        <  4:309626/148‑1 (MQ=255)
                                                                          aCCGCGTTCGTCGGCCACCAGTACCACGTCGTACTCGGCAGTCTGGGTGAACAGCGGCATGTCGGCCTGGGCGCTGCGGCGCTGGTCGTTGTCGAAGGTCCAGGTTTTTTCCGCGACCAGTTGCACGCCAAAGCGCTTCACCGAccgcc                                                                       >  6:341637/1‑149 (MQ=255)
                                                                               gTTCGTCGGCCACCAGTACCACGTCGTACTCGGCAGGCTGGGTGAACAGCGGCATGTCGGCCTGGGCGCTGCGGCGCTGGTCGTTGTCGAAGGTCCAGGTTTTTTCAGCGACCAGTTGCACGCCAAAGCGCTTCACCGACCGCCGCAgg                                                                  >  6:91849/1‑149 (MQ=255)
                                                                                       gCCTCCAGTACCACGTCGTACTCGGCAGTCTGGGTGAACAGCGGCATGTCGGCCTGGGCGCTGCGGCGCTGGTCGTTGTCGAAGGTCCAGGTTTTTTCCGCGACCAGTTGCACGCCAAAGCGCTTCACCGACCGCCGCAGGGCAgcggc                                                          <  5:109985/149‑1 (MQ=255)
                                                                                       gCCACCAGTACCACGTCGTACTCGGCAGTCTGGGTGAACAGCGGCATGTCGGCCTGGGCGCTGCGGCGCTGGTCGTTGTCGAAGGTCCAGGTTTTTTCCGCGACCAGTTGCACGCCAAAGCGCTTCACCGACCGCCGCAGGGCAgcggc                                                          <  3:139696/149‑1 (MQ=255)
                                                                                         caccaGTACCACGTCGTACTCGGCAGTCTGGGTGAACAGCGGCATGTCGGCCTGGGCGCTGCGGCGCTGGTCGTTGTCGAAGGTCCAGGTTTTTTCCGCGACCAGtt                                                                                                  <  6:41360/107‑1 (MQ=255)
                                                                                         caccaGTACCACGTCGTACTCGGCAGTCTGGGTGAACAGCGGCATGTCGGCCTGGGCGCTGCGGCGCTGGTCGTTGTCGAAGGTCCAGGTTTTTTCCGCGACCAGtt                                                                                                  >  5:41360/1‑107 (MQ=255)
                                                                                            caGTACCACGTCGTACTCGGCAGTCTGGGTGAACAGCGGCATGTCGGCCTGGGCGCTGCGGCGCTGGTCGTTGTCGAAGGTCCAGGTTTTTTCCGCGACCAGTTGCACGCCAAAGCGCTTCACCGACCGCCGCAGGGCAGCGGCGTACg                                                     <  8:287512/149‑1 (MQ=255)
                                                                                            caGTACCACGTCGTACTCGGCAGTCTGGGTGAACAGCGGCATGTCGGCCTGGGCGCTGCGGCGCTGGTCGTTGTCGAAGGTCCAGGTTTTTTCCGCGACCAGTTGCACGCCAAAGCGCTTCACCGACCGCCGCAGGGCAGCGGCGTACg                                                     >  6:120250/1‑149 (MQ=255)
                                                                                             aGTACCACGTCGTACTCGGCAGTCTGGGTGAACAGCGGCATGTCGGCCTGGGCGCTGCGGCGCTGGTCGTTGTCGAAGGTCCAGGTTTTTTCCGCGACCAGTTGCACGCCAAAGCGCTTCACCGACCGCCGCAGGGCAGCGGCGTACGc                                                    <  6:341569/149‑1 (MQ=255)
                                                                                               tACCACGTCGTACTCGGCAGTCTGGGTGAACAGCGGCATGTCGGCCTGGGCGCTGCGGCGCTGGTCGTTGTCGAAGGTCCAGGTTTTTTCCGCGACCAGTTGCACGCCAAAGCGCTTCACCGACCGCCGCAGGGCAGCGGCGTACGCCt                                                  <  5:120250/149‑1 (MQ=255)
                                                                                               tACCACGTCGTACTCGGCAGTCTGGGTGAACAGCGGCATGTCGGCCTGGGCGCTGCGGCGCTGGTCGTTGTCGAAGGTCCAGGTTTTTTCCGCGACCAGTTGCACGCCAAAGCGCTTCACCGACCGCCGCAGGGCAGCGGCGTACGCCt                                                  <  3:102730/149‑1 (MQ=255)
                                                                                                 ccACGTCGTACTCGGCAGTCTGGGTGAACAGCGGCATGTCGGCCTGGGCGCTGCGGCGCTGGTCGTTGTCGAAGGTCCAGGTTTTTTCCGCGACCAGTTGCACGCCAAAGCGCTTCACCGACCGCCGCAGGGCAGCGGCGTACGCCTGa                                                <  2:212363/149‑1 (MQ=255)
                                                                                                  cACGTCGTACTCGGCAGTCTGGGTGAACAGCGGCATGTCGGCCTGGGCGCTGCGGCGCTGGTCGTTGTCGAAGGTCCAGGTTTTTTCCGCGACCAGTTGCACGCCAAAGCGCTTCaccga                                                                            <  1:138792/120‑1 (MQ=255)
                                                                                                  cACGTCGTACTCGGCAGTCTGGGTGAACAGCGGCATGTCGGCCTGGGCGCTGCGGCGCTGGTCGTTGTCGAAGGTCCAGGTTTTTTCCGCGACCAGTTGCACGCCAAAGCGCTTCaccga                                                                            >  2:138792/1‑120 (MQ=255)
                                                                                                     gtcgtACTCGGCAGTCTGGGTGAACAGCGGCATGTCGGCCTGGGCGCTGCGGCGCTGGTCGTTGTCGAAGGTCCAGGTTTTTTCCGCGACCAGTTGCACGCCAAAGCGCTTCACCGACCGCCGCAGGGCAGCGGCGTACGCCTGATCGt                                            >  5:261045/1‑149 (MQ=255)
                                                                                                           cTCGGCAGTCTGGGTGAACAGCGGCATGTCGGCCTGGGCGCTGCGGCGCTGGTCGTTGTCGAAGGTCCAGGTTTTTTCCGCGACCAGTTGCACGCCAAAGCGCTTCACCGACCGCCGCAGGGCAGCGGCGTACGCCTGATCGTCCggg                                       <  2:208558/148‑1 (MQ=255)
                                                                                                           cTCGGCAGTCTGGGTGAACAGCGGCATGTCGGCCTGGGCGCTGCGGCGCTGGTCGTTGTCGAAGGTCCAGGTTTTTTCCGCGACCAGTTGCACGCCAAAGCGCTTCACCGACCGCCGCAGGGCAACGGCGTACGCCTGATCGTCCggg                                       <  8:314763/148‑1 (MQ=255)
                                                                                                            tCGGCAGTCTGGGTGAACAGCGGCATGTCGGCCTGGGCGCTGCGGCGCTGGTCGTTGTCGAAGGTCCAGGTTTTTTCCGCGACCAGTTGCACGCCAAAGCGCTTCACCGACCGCCGCAGGGCAGCGGCGTACGCCTGATCGTCCgggg                                      >  5:193285/1‑148 (MQ=255)
                                                                                                                cAGTCTGGGTGAACAGCGGCATGTCGGCCTGGGCGCTGCGGCGCTGGTCGTTGTCGAAGGTCCAGGTTTTTTCCGCGACCAGTTGCACGCCAAAGCGCTTCACCGACCGCCGCAGGGCAGCGGCGTACGCCTGATCGTCCGGGGTCTgg                                 <  8:203292/149‑1 (MQ=255)
                                                                                                                  gTCTGGGTGAACAGCGGCATGTCGGCCTGGGCGCTGCGGCGCTGGTCGTTGTCGAAGGTCCAGGTTTTTTCCGCGACCAGTTGCACGCCAAAGCGCTTCACCGACCGCCGCAGGGCAGCGGCGTACGCCTGATCGTCCGGGGTCTGGcc                               <  4:62167/149‑1 (MQ=255)
                                                                                                                        gTGAACAGCGGCATGTCGGCCTGGGCGCTGCGGCGCTGGTCGTTGTCGAAGGTCCAGGTTTTTTCCGCGACCAGTTGCACGCCAAAGCGCTTCACCGACCGCCGCAGGGCAGCGGCGTACGCCTGATCGTCCGGGGTCTGGCCGaccac                         <  1:48233/149‑1 (MQ=255)
                                                                                                                                   cATGTCGGCCTGGGCGCTGCGGCGCTGGTCGTTGTCGAAGGTCCAGGTTTTTTCCGCGACCAGTTGCACGCCAAAGCGCTTCACCGACCGCCGCAGGGCAGCGGCGTACGCCTGATCGTCCGGGGTCTGGCCGACCACCAGCAATGccc              <  3:107678/149‑1 (MQ=255)
                                                                                                                                       tCGGCCTGGGCGCCGCGGCGCTGGTCGTTGTCGAAGGTCCAGGTTTTTTCCGCGACCAGTTGCACGCCAAAGCGCTTCACCGACCGCCGCAGGGCAGCGGCGTACGCCTGATCGTCCGGGGt                                     <  5:52951/122‑1 (MQ=255)
                                                                                                                                       tCGGCCTGGGCGCCGCGGCGCTGGTCGTTGTCGAAGGTCCAGGTTTTTTCCGCGACCAGTTGCACGCCAAAGCGCTTCACCGACCGCCGCAGGGCAGCGGCGTACGCCTGATCGTCCGGGGt                                     >  6:52951/1‑122 (MQ=255)
                                                                                                                                       tCGGCCTGGGCGCCGCGGCGCTGGTCGTTGTCGAAGGTCCAGGTTTTTTCCGCGACCAGTTGCACGCCAAAGCGCTTCACCGACCGCCGCAGGGCAGCGGCGTACGCCTGATCGTCCGGGGt                                     <  7:241103/122‑1 (MQ=255)
                                                                                                                                       tCGGCCTGGGCGCCGCGGCGCTGGTCGTTGTCGAAGGTCCAGGTTTTTTCCGCGACCAGTTGCACGCCAAAGCGCTTCACCGACCGCCGCAGGGCAGCGGCGTACGCCTGATCGTCCGGGGt                                     >  8:241103/1‑122 (MQ=255)
                                                                                                                                          gCCTGGGCGCTGCGGCGCTGGTCGTTGTCGAAGGTCCAGGTTTTTTCCGCGACCAGTTGCACGCCAAAGCGCTTCACCGACCGCCGCAGGGCAGCGGCGTACGCCTGATCGTCCGGGGTCTGGCCGACCACCAGCAATGc                <  1:238986/140‑1 (MQ=255)
                                                                                                                                          gCCTGGGCGCTGCGGCGCTGGTCGTTGTCGAAGGTCCAGGTTTTTTCCGCGACCAGTTGCACGCCAAAGCGCTTCACCGACCGCCGCAGGGCAGCGGCGTACGCCTGATCGTCCGGGGTCTGGCCGACCACCAGCAATGc                >  2:238986/1‑140 (MQ=255)
                                                                                                                                          gCCTGGGCGCTGCGGCGCTGGTCGTTGTCGAAGGTCCAGGTTTTTTCCGCGACCAGTTGCACGCCAAAGCGCTTCACCGACCGCCGCAGGGCAGCGGCGTACGCCTGATCGTCCGGGGTCTGGCCGACCACCAGCAATGCCCGtt           <  3:263418/145‑1 (MQ=255)
                                                                                                                                          gCCTGGGCGCTGCGGCGCTGGTCGTTGTCGAAGGTCCAGGTTTTTTCCGCGACCAGTTGCACGCCAAAGCGCTTCACCGACCGCCGCAGGGCAGCGGCGTACGCCTGATCGTCCGGGGTCTGGCCGACCACCAGCAATGCCCGtt           >  4:263418/1‑145 (MQ=255)
                                                                                                                                               ggCGCTGCGGCGCTGGTCGTTGTCGAAGGTCCAGGTTTTTTCCGCGACCAGTTGCACGCCAAAGCGCTTCACCGACCGCCGCAGGGCAGCGGCGTACGCCTGATCGTCCGGGGTCTGGCGGACCACCAGCAATGCCCGTTGCCATTTgc  >  5:302098/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                               
GTGGGGGTCAGGCCCTGGGTACCGGCCACCGGACGTGGATACCACGTCTGGTAGGGCAGGTACTCGCCGAAGTCACCGCGTTCGTCGGCCACCAGTACCACGTCGTACTCGGCAGTCTGGGTGAACAGCGGCATGTCGGCCTGGGCGCTGCGGCGCTGGTCGTTGTCGAAGGTCCAGGTTTTTTCCGCGACCAGTTGCACGCCAAAGCGCTTCACCGACCGCCGCAGGGCAGCGGCGTACGCCTGATCGTCCGGGGTCTGGCCGACCACCAGCAATGCCCGTTGCCATTTGC  >  NC_002947/3057760‑3058051

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑