Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,152,237 T→G 7.3% L131R (CTG→CGG)  PP_2768 → branched‑chain amino acid ABC transporter permease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,152,2370TG 7.3% 148.9 / 2.4 51L131R (CTG→CGG) PP_2768branched‑chain amino acid ABC transporter permease
Reads supporting (aligned to +/- strand):  ref base T (23/24);  new base G (2/2);  total (25/26)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.65e-01

GTACTGCGGCCATTGGTCGGGCGTTCGCCGATAACCCTGTTCATGGCCACGCTCGGCCTGGCCTACATCATCGAGGGCGCCGCCCAGGCCTTATGGGGCGCCCAGGTTCACGGCCTGGAGCTGGCCATCAGCGACGCCCCGCTGGAGCTGGGCGGCCTGCTGCTGTCGCAGTTCGACCTGTTCGCCGCCGCAACGGCGGCGGCCCTGGTGGTGCTTCTGTCCTTGCTGTTCAACCGCACGCGCATCGGCCTGGCGCTGCGTGCGGTGGCCGACGATCCCCGCGCGGCGATGA  >  NC_002947/3152089‑3152380
                                                                                                                                                    |                                                                                                                                               
gTACTGCGGCCATTGGTCGGGCGTTCGCCGATAACCCTGTTCATGGCCACGCTCGGCCTGGCCTACATCATCGAGGGCGCCGCCCAGGCCTTATGGGGCGCCCAGGTTCACGGCCTGGAGCTGGCCATCAGCGACGCCCCGCTGGAGCt                                                                                                                                                 <  8:282792/149‑1 (MQ=255)
   cTGCGGCCATTGGTCGGGCGTTCGCCGATAACCCTGTTCATGGCCACGCTCGGCCTGGCCTACATCATCGAGGGCGCCGCCCAGGCCTTATGGGGCGCCCAGGTTCACGGCCTGGAGCTGGCCATCAGCGACGCCCCGCTGGAGCTgg                                                                                                                                               <  2:296998/148‑1 (MQ=255)
      cGGCCATTGGTCGGGCGTTCGCCGATAACCCTGTTCATGGCCACGCTCGGCCTGGCCTACATCATCGAGGGCGCCGCCCAGGCCTTATGGGGCGCCCAGGTTCACGGCCTGGAGCTGGCCATCAGCGACGCCCCGCTGGAGCTGggc                                                                                                                                             <  1:178378/147‑1 (MQ=255)
        gCCATTGGTCGGGCGTTCGCCGATAACCCTGTTCATGGCCACGCTCGGCCTGGCCTACATCATCGAGGGCGCCGCCCAGGCCTTATGGGGCGCCCAGGTTCACGGCCTGGAGCTGGCCATCAGCGACGCCCCGCTGGAGCTGGGCGGcc                                                                                                                                         >  3:133571/1‑149 (MQ=255)
          cATTGGTCGGGCGTTCGCCGATAACCCTGTTCATGGCCACGCTCGGCCTGGCCTACATCATCGAGGGCGCCGCCCAGGCCTTATGGGGCGCCCAGGTTCACGGCCTGGAGCTGGCCATCAGCGACGCCCCGCTGGAGCTGGGCGGCctg                                                                                                                                       >  3:289571/1‑149 (MQ=255)
           aTTGGTCGGGCGTTCGCCGATAACCCTGTTCATGGCCACGCTCGGCCTGGCCTACATCATCGAGGGCGCCGCCCAGGCCTTATGGGGCGCCCAGGTTCACGGCCTGGAGCTGGCCATCAGCGACGCCCCGCTGGAGCTGGGCGGCctgc                                                                                                                                      >  4:298550/1‑149 (MQ=255)
                tCGGGCGTTCGCCGATAACCCTGTTCATGGCCACGCTCGGCCTGGCCTACATCATCGAGGGCGCCGCCCAGGCCTTATGGGGCGCCCAGGTTCACGGCCTGGAGCTGGCCATCAGCGACGCCCCGCTGGAGCTGGGCGGCctgctgctg                                                                                                                                 >  1:309267/1‑149 (MQ=255)
                     cGTTCGCCGATAACCCTGTTCATGGCCACGCTCGGCCTGGCCTACATCATCGAGGGCGCCGCCCAGGCCTTATGGGGCGCCCAGGTTCACGGCCTGGAGCTGGCCATCAGCGACGCCCCGCTGGAGCTGGGCGGCCTGCTGCTGTCGCa                                                                                                                            <  3:298550/149‑1 (MQ=255)
                         cGCCGATAACCCTGTTCATGGCCACGCTCGGCCTGGCCTACATCATCGAGGGCGCCGCCCAGGCCTTATGGGGCGCCCAGGTTCACGGCCTGGAGCTGGCCATCAGCGACGCCCCGCTGGAGCTGGGCGGCCTGCTGCTGTCGCAGtt                                                                                                                         <  4:133571/148‑1 (MQ=255)
                          gCCGATAACCCTGTTCATGGCCACGCTCGGCCTGGCCTACATCATCGAGGGCGCCGCCCAGGCCTTATGGGGCGCCCAGGTTCACGGCCTGGAGCTGGCCATCAGCGACGCCCCGCTGGAGCTGGGCGGCctgctg                                                                                                                                    <  4:300563/136‑1 (MQ=255)
                          gCCGATAACCCTGTTCATGGCCACGCTCGGCCTGGCCTACATCATCGAGGGCGCCGCCCAGGCCTTATGGGGCGCCCAGGTTCACGGCCTGGAGCTGGCCATCAGCGACGCCCCGCTGGAGCTGGGCGGCctgctg                                                                                                                                    >  3:300563/1‑136 (MQ=255)
                          gCCGATAACCCTGTTCATGGCCACGCTCGGCCTGGCCTACATCATCGAGGGCGCCGCCCAGGCCTTATGGGGCGCCCAGGTTCACGGCCTGGAGCTGGCCATCAGCGACGCCCCGCTGGAGCTGGGCGGCCTGCTGCTGTCGCAGTTc                                                                                                                        >  8:154854/1‑148 (MQ=255)
                            cGATAACCCTGTTCATGGCCACGCTCGGCCTGGCCTACATCATCGAGGGCGCCGCCCAGGCCTTATGGGGCGCCCAGGTTCACGGCCTGGAGCTGGCCATCAGCGACGCCCCGCTGGAGCTGGGCGGCCTGCTGCTGTCGCAGTTCGAc                                                                                                                     <  5:248452/149‑1 (MQ=255)
                              aTAACCCTGTTCATGGCCACGCTCGGCCTGGCCTACATCATCGAGGGCGCCGCCCAGGCCTTATGGGGCGCCCAGGTTCACGGCCTGGAGCTGGCCATCAGCGACGCCCCGCTGGAGCTGGGCGGCCTGCTGCTGTCGCAGTTCGACCt                                                                                                                   >  5:277989/1‑149 (MQ=255)
                                aaCCCTGTTCATGGCCACGCTCGGCCTGGCCTACATCATCGAGGGCGCCGCCCAGGCCTTATGGGGCGCCCAGGTTCACGGCCTGGAGCTGGCCATCAGCGACGCCCCGCTGGAGCTGGGCGGCCTGCTGCTGTCGCAGTTCGACCTGt                                                                                                                 >  4:215748/1‑149 (MQ=255)
                                 aCCCTGTTCATGGCCACGCTCGGCCTGGCCTACATCATCGAGGGCGCCGCCCAGGCCTTATGGGGCGCCCAGGTTCACGGCCTGGAGCTGGCCATCAGCGACGCCCCGCTGGAGCTGGGCGGCCTGCTGCTGTCGCAGTTCGACCTGt                                                                                                                 <  4:264812/148‑1 (MQ=255)
                                       ttCATGGCCACGCTCGGCCTGGCCTACATCATCGAGGGCGCCGCCCAGGCCTTATGGGGCGCCCAGGTTCACGGCCTGGAGCTGGCCATCAGCGACGCCCCGCTGGAGCTGGGCGGCCTGCTGCTGTCGCAGTTCGACCTGTTcgccgc                                                                                                          >  8:186939/1‑149 (MQ=255)
                                                          tggcctACATCATCGAGGGCGCCGCCCAGGCCTTATGGGGCGCCCAGGTTCACGGCCTGGAGCTGGCCATCAGCGACGCCCCGCTGGAGCTGGGCGGCCTGCTGCTGTCGCAGTTCGACCTGTTCGCCGCCGCAACGGCGGCGGCCCtg                                                                                       <  4:289571/149‑1 (MQ=255)
                                                            gcctACATCATCGAGGGCGCCGCCCTGGCCTTATGGGGCGCCCAGGTTCAGGGCCGGGAGCTGGCCATCAGCGACGCCCCGCTGGAGCGGGGCGGCCTGCTGCTGTCGCAGTGCGACCTGTTCGCCGCCGCAACGGGGGCGGCCCtggt                                                                                     <  8:293706/149‑1 (MQ=255)
                                                             cttACATCATCGAGGGCGCCGCCCAGGCCTTAGGGGGCGCCCAGGTTCACGGCCTGGAGCTGGCCATCAGCGACGCCCCGCTGGAGCTGGGCGGCCTGCTGCTGTCGCAGTTCGACCTGTTCGCCGCCGCAACGGCGGCGGCCCtggtg                                                                                    <  1:303896/147‑1 (MQ=255)
                                                             cctACATCATCGAGGGCGCCGCCCAGGCCTTATGGGGCGCCCAGGTTCACGGCCTGGAGCTGGCCATCAGCGACGCCCCGCTGGAGCTGGGCGGCCTGCTGCTGTCGCAGTTCGACCTGTTCGCCGCCGCAACGGCGGCGGCCCtggtg                                                                                    <  7:154854/149‑1 (MQ=255)
                                                              ctACATCATCGAGGGCGCCGCCCAGGCCTTATGGGGCGCCCAGGTTCACGGCCTGGAGCTGGCCATCAGCGACGCCCCGCTGGAGCTGGGCGGCCTGCTGCTGTCGCAGTTCGACCTGTTCGCCGCCGCAACGGCGGCGGCCCtggtgg                                                                                   <  7:218268/149‑1 (MQ=255)
                                                              ctACATCATCGAGGGCGCCGCCCAGGCCTTATGGGGCGCCCAGGTTCACGGCCTGGAGCTGGCCATCAGCGACGCCCCGCTGGAGCTGGGCGGCCTGCTGCTGTCGCAGTTCGACCTGTTCGCCGCCGCAACGGCGGCGGCCCtggtgg                                                                                   >  1:200239/1‑149 (MQ=255)
                                                                aCATCATCGAGGGCGCCGCCCAGGCCTTATGGGGCGCCCAGGTTCACGGCCTGGAGCTGGCCATCAGCGACGCCCCGCTGGAGCTGGGCGGCCTGCTGCTGTCGCAGTTCGACCTGTTCGCCGCCGCAACGGCGGCGGCCCTGgtggtg                                                                                 <  8:243790/149‑1 (MQ=255)
                                                                aCATCATCGAGGGCGCCGCCCAGGCCTTATGGGGCGCCCAGGTTCACGGCCTGGAGCTGGCCATCAGCGACGCCCCGCTGGAGCTGGGCGGCCTGCTGCTGTCGCAGTTCGACCTGTTCGCCCCCCCAACCGCGGCGGCCCagggg                                                                                    >  2:303896/1‑144 (MQ=255)
                                                                 catcatCGAGGGCGCCGCCCAGGCCTTATGGGGCGCCCAGGTTCACGGCCTGGAGCTGGCCATCAGCGACGCCCCGCTGGAGCTGGGCGGCCTGCTGCTGTCGCAGTTCGACCTGTTCGCCGCCGCAACGGCGGCGGCCCTggtggt                                                                                  <  6:89887/147‑1 (MQ=255)
                                                                     atcGAGGGCGCCGCCCAGGCCTTATGGGGCGCCCAGGTTCACGGCCTGGAGCTGGCCATCAGCGACGCCCCGCTGGAGCTGGGCGGCCTGCTGCTGTCGCAGTTCGACCTGTTCGCCCCCCGAACGGCCGCCGCCCgggtgggtgatgg                                                                            >  2:249153/1‑142 (MQ=255)
                                                                         aGGGCGCCGCCCAGGCCTTATGGGGCGCCCAGGTTCACGGCCTGGAGCTGGCCATCAGCGACGCCCCGCTGGAGCTGGGCGGCCTGCTGCTGTCGCAGTTCGACCTGTTCGCCGCCGCAACGGCGGCGGCCCTGGTGGTGCTTCTGTcc                                                                        <  8:6516/149‑1 (MQ=255)
                                                                         aGGGCGCCGCCCAGGCCTTATGGGGCGCCCAGGTTCACGGCCTGGAGCTGGCCATCAGCGACGCCCCGCTGGAGCTGGGCGGCCTGCTGCTGTCGCAGTTCGACCTGTTCGCCGCCGCAACGGCGGCGGCCCTGGTGGTGCTTCTGTcc                                                                        >  4:263894/1‑149 (MQ=255)
                                                                            gcgcCGCCCAGGCCTTATGGGGCGCCCAGGTTCACGGCCTGGAGCTGGCCATCAGCGACGCCCCGCTGGAGCTGGGCGGCCTGCTGCTGTCGCAGTTCGACCTGTTCGCCGCCGCAACGGCGGCGGCCCTGGTGGTGCTTCTGTCCTTg                                                                     <  3:215748/149‑1 (MQ=255)
                                                                              gccgccCAGGCCTTATGGGGCGCCCAGGTTCACGGCCTGGAGCTGGCCATCAGCGACGCCCCGCTGGAGCTGGGCGGCCTGCTGCTGTCGCAGTTCGACCTGTTCGCCGCCGCAACGGCGGCGGCCCTGGTGGTGCTTCTGTCCTTGCt                                                                   >  1:195655/1‑149 (MQ=255)
                                                                              gccgccCAGGCCTTATGGGGCGCCCAGGTTCACGGCCTGGAGCTGGCCATCAGCGACGCCCCGCTGGAGCTGGGCGGCCTGCTGCTGTCGCAGTTCGACCTGTTCGCCGCCGCAACGGCGGCGGCCCTGGTGGTGCTTCTGTCCTTGCt                                                                   <  4:233818/149‑1 (MQ=255)
                                                                              gccgccCAGGCCTTATGGGGCGCCCAGGTTCACGGCCTGGAGCTGGCCATCAGCGACGCCCCGCTGGAGCTGGGCGGCCTGCTGCTGTCGCAGTTCGACCTGTTCGCCGCCGCAACGGCGGCGGCCCTGGTGGTGCTTCTGTCCTTGCt                                                                   >  7:15389/1‑149 (MQ=255)
                                                                               ccgccCAGGCCTTATGGGGCGCCCAGGTTCACGGCCTGGCGCTGGCCATCAGCGACGCCCCGCTGGAGCTGGGCGGCCTGCTGCTGTCGCAGTTCGACCTGTTCGCCGCCGCAACGGCGGCGGCCCTGGTGGTGCTTCTGTCCTTGCTg                                                                  <  8:237625/149‑1 (MQ=255)
                                                                                  cccAGGCCTTATGGGGCGCCCAGGTTCACGGCCTGGAGCTGGCCATCAGCGACGCCCCGCTGGAGCTGGGCGGCCTGCTGCTGTCGCAGTTCGACCTGTTCGCCGCCGCAACGGCGGCGGCCCTGGTGGTGCTTCTGTCCTTGCTGtt                                                                >  7:123922/1‑148 (MQ=255)
                                                                                  cccAGGCCTTATGGGGCGCCCAGGTTCACGGCCTGGAGCTGGCCATCAGCGACGCCCCGCTGGAGCTGGGCGGCCTGCTGCTGTCGCAGTTCGACCTGTTCGCCGCCGCAACGGCGGCGGCCCTGGTGGTGCTTCTGTCCTTGCTGTTc                                                               <  8:123922/149‑1 (MQ=255)
                                                                                    cAGGCCTTATGGGGCGCCCAGGTTCACGGCCTGGAGCTGGCCATCAGCGACGCCCCGCTGGAGCTGGGCGGCCTGCTGCTGTCGCAGTTCGACCTGTTCGCCGCCGCAACGGCGGCGGCCCTGGTGGTGCTTCTGTCCTTGCTGTTCaa                                                             >  2:216906/1‑149 (MQ=255)
                                                                                          ttATGGGGCGCCCAGGTTCACGGCCTGGAGCTGGCCATCAGCGACGCCCCGCTGGAGCTGGGCGGCCTGCTGCTGTCGCAGTTCGACCTGTTCGCCGCCGCAACGGCGGCGGCCCTGGTGGTGCTTCTGTCCTTGCTGTTCAACCGCAc                                                       >  4:312278/1‑149 (MQ=255)
                                                                                                ggCGCCCAGGTTCACGGCCTGGAGCTGGCCATCAGCGACGCCCCGCTGGAGCTGGGCGGCCTGCTGCTGTCGCAGTTCGACCTGTTCGCCGCCGCAACGGCGGCGGCCCTGGTGGTGCTTCTGTCCTTGCTGTTCAACCGCACGCGCAt                                                 <  3:263894/149‑1 (MQ=255)
                                                                                                 gcgcCCAGGTTCACGGCCTGGAGCTGGCCATCAGCGACGCCCCGCTGGAGCTGGGCGGCCTGCTGCTGTCGCAGTTCGACCTGTTCGCCGCCGCAACGGCGGCGGCCCTGGTGGTGCTTCTGTCCTTGCTGTTCAACCGCACGCGCATc                                                >  6:249803/1‑149 (MQ=255)
                                                                                                  cgcCCAGGTTCACGGCCTGGAGCTGGCCATCAGCGACGCCCCGCTGGAGCTGGGCGGCCTGCTGCTGTCGCAGTTCGACCTGTTCGCCGCCGCAACGGCGGCGGCCCTGGTGGTGCTTCTGTCCTTGCTGTTCAACCGCACGCGCATCg                                               >  8:28438/1‑149 (MQ=255)
                                                                                                     ccAGGTTCACGGCCTGGAGCTGGCCATCAGCGACGCCCCGCTGGAGCTGGGCGGCCTGCTGCTGTCGCAGTTCGACCTGTTCGCCGCCGCAACGGCGGCGGCCCTGGTGGTGCTTCTGTCCTTGCTGTTCAACCGCACGCGCATCGGcc                                            >  3:75949/1‑149 (MQ=255)
                                                                                                              cGGCCTGGAGCTGGCCATCAGCGACGCCCCGCTGGAGCTGGGCGGCCTGCTGCTGTCGCAGTTCGACCTGTTCGCCGCCGCAACGGCGGCGGCCCTGGTGGTGCTTCTGTCCTTGCTGTTCAACCGCACGCGCATCGGCCTGGCGCtgc                                   <  7:186939/149‑1 (MQ=255)
                                                                                                                     gAGCTGGCCATCAGCGACGCCCCGCTGGAGCTGGGCGGCCTGCTGCTGTCGCAGTTCGACCTGTTCGCCGCCGCAACGGCGGCGGCCCTGGTGGTGCTTCTGTCCTTGCTGTTCAACCGCACGCGCATCGGCCTGGCGCTGCGTGCGGt                            <  4:75949/149‑1 (MQ=255)
                                                                                                                     gAGCTGGCCATCAGCGACGCCCCGCTGGAGCTGGGCGGCCTGCTGCTGTCGCAGTTCGACCTGTTCGCCGCCGCAACGGCGGCGGCCCTGGTGGTGCTTCTGTCCTTGCTGTTCAACCGCACGCGCATCGGCCTGGCGCTGCGTGCGGt                            <  5:249803/149‑1 (MQ=255)
                                                                                                                           gCCATCAGCGACGCCCCGCTGGAGCTGGGCGGCCTGCTGCTGTCGCAGTTCGACCTGTTCGCCGCCGCAACGGCGGCGGCCCTGGTGGTGCTTCTGTCCTTGCTGTTCAACCGCACGCGCATCGGCCTGGCGCTGCGTGCGGTGGcc                        <  2:3615/147‑1 (MQ=255)
                                                                                                                             cATCAGCGACGCCCCGCTGGAGCTGGGCGGCCTGCTGCTGTCGCAGTTCGACCTGTTCGCCGCCGCAACGGCGGCGGCCCTGGTGGTGCTTCTGTCCTTGCTGTTCAACCGCACGCGCATCGGCCTGGCGCTGCGTGCGGTGGCcgac                     >  5:13032/1‑148 (MQ=255)
                                                                                                                               agcaggccGCCCAGCTCCAGCGGGGCGGCCTGCTGCTGTCGCAGTTCGACCTGTTCGCCGCCGCAACGGCGGCGGCCCTGGTGGTGCTTCTGTCCTTGCTGTTCAACCGCACGCGCATCGGCCTGGCGCTGCGTGCGGTGGCCGACGAt                  >  6:108844/8‑149 (MQ=255)
                                                                                                                                      cGCCCAGCTCCAGCGGGGCGGCCTGCTGCTGTCGCAGTTCGACCTGTTCGCCGCCGCAACGGCGGCGGCCCTGGTGGTGCTTCTGTCCTTGCTGTTCAACCGCACGCGCATCGGCCTGGCGCTGCGTGCGGTGGCCGACGATcc                >  7:28133/1‑144 (MQ=255)
                                                                                                                                      cGCCCAGCTCCAGCGGGGCGGCCTGCTGCTGTCGCAGTTCGACCTGTTCGCCGCCGCAACGGCGGCGGCCCTGGTGGTGCTTCTGTCCTTGCTGTTCAACCGCACGCGCATCGGCCTGGCGCTGCGTGCGGTGGCCGACGATcc                <  8:28133/144‑1 (MQ=255)
                                                                                                                                            gCTGGAGCTGGGCGGCCTGCTGCTGTCGCAGTTCGACCTGTTCGCCGCCGCAACGGCGGCGGCCCTGGTGGTGCTTCTGTCCTTGCTGTTCAACCGCACGCGCATCGGCCTGGCGCTGCGTGCGGTGGCCGACGATCCCCGCGCGGCGa     >  3:146788/1‑149 (MQ=255)
                                                                                                                                                gAGCTGGGCGGCCTGCTGCTGTCGCAGTTCGACCTGTTCGCCGCCGCAACGGCGGCGGCCCTGGTGGTGCTTCTGTCCTTGCTGTTCAACCGCACGCGCATCGGCCTGGCGCTGCGTGCGGTGGCCGACGATCCCCGCGCGGCGATGa  <  1:216906/148‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                               
GTACTGCGGCCATTGGTCGGGCGTTCGCCGATAACCCTGTTCATGGCCACGCTCGGCCTGGCCTACATCATCGAGGGCGCCGCCCAGGCCTTATGGGGCGCCCAGGTTCACGGCCTGGAGCTGGCCATCAGCGACGCCCCGCTGGAGCTGGGCGGCCTGCTGCTGTCGCAGTTCGACCTGTTCGCCGCCGCAACGGCGGCGGCCCTGGTGGTGCTTCTGTCCTTGCTGTTCAACCGCACGCGCATCGGCCTGGCGCTGCGTGCGGTGGCCGACGATCCCCGCGCGGCGATGA  >  NC_002947/3152089‑3152380

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑