Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,152,451 C→T 8.5% A202A (GCC→GCT)  PP_2768 → branched‑chain amino acid ABC transporter permease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,152,4510CT 8.5% 132.3 / 2.5 47A202A (GCC→GCT) PP_2768branched‑chain amino acid ABC transporter permease
Reads supporting (aligned to +/- strand):  ref base C (22/21);  new base T (2/2);  total (24/23)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01

CTTGCTGTTCAACCGCACGCGCATCGGCCTGGCGCTGCGTGCGGTGGCCGACGATCCCCGCGCGGCGATGAGCGTCGGTATCCGCCTGCCGAGGGTGTGGGCGGTGGTCTGGGCGGTGGCCGGGTTTGTCGGGTTGGTGGCCGGGCTGCTCTGGGGCGCACGCCTGGGGGTGCAGTTCTCGCTCTCGCTGGTGGTGCTCAAGGCCCTGCCGGTGCTGATCATCGGCGGTTTTACCTCGATCAGCGGGGCGATCGTCGGCGGCCTGATCATCGGCGCTTCGGAGAAGCTC  >  NC_002947/3152310‑3152598
                                                                                                                                             |                                                                                                                                                   
cTTGCTGTTCAACCGCACGCGCATCGGCCTGGCGCTGCGTGCGGTGGCCGACGATCCCCGCGCGGCGATGAGCGTCGGTATCCGCCTGCCGAGGGTGTGGGCGGTGGTCTGGGCGGTGGCCGGGTTTGTCGGGTTGGTGGCCGGgctgc                                                                                                                                              <  4:140170/149‑1 (MQ=255)
cTTGCTGTTCAACCGCACGCGCATCGGCCTGGCGCTGCATGCGGTGACCGACGATCCCAGCGCGGCGATGAGCGTCGGTATCCGCATGCAGAGGGTGGGGTAGGGGGTCTGGGCGGTGGCCGTGTATGTAGGGTTGGTTGCCGGgctgc                                                                                                                                              >  8:209557/1‑149 (MQ=255)
  tGCTGTTCAACCGCACGCGCATCGGCCTGGCGCTGCGTGCGGTGGCCGACGATCCCCGCGCGGCGATGAGCGTCGGTATCCGCCTGCCGAGGGTGTGGGCGGTGGTCTGGGCGGTGGCCGGGTTTGTCGGGTTGGTGGCCGGGCTGctc                                                                                                                                            >  2:26325/1‑149 (MQ=255)
    cTGTTCAACCGCACGCGCATCGGCCTGGCGCTGCGTGCGGTGGCCGACGATCCCCGCGCGGCGATGAGCGTCGGTATCCGCCTGCCGAGGGTGTGGGCGGTGGTCTGGGCGGGGGCCGGGTTTGTCGGGTTGGTGGCCGGGCTGctct                                                                                                                                           <  2:195655/148‑1 (MQ=255)
     tGTTCAACCGCACGCGCATCGGCCTGGCGCTGCGTGCGGTGGCCGACGATCCCCGCGCGGCGATGAGCGTCGGTATCCGCCTGCCGAGGGTGTGGGCGGTGGTCTGGGCGGTGGCCGGGTTTGTCGGGTTGGTGGCCGGGCTGCTCTgg                                                                                                                                         >  8:286544/1‑149 (MQ=255)
       ttCAACCGCACTCGCAGCGGCCTGGCGCTGCGTGCGGTGGCCGACGATCCACGCGCGGCGATGAGCGTCGGTATCCGCCTGCCGAGGGTGGGGGCGGTGGTCTGGGCGGTGGCCGGGTTTGTCGGGTTGGTGGCCGGGCTGCTCTgg                                                                                                                                         <  5:28876/147‑1 (MQ=255)
         cAACCGCACGCGCATCGGCCTGGCGCTGCGTGCGGTGGCCGACGATCCCCGCGCGGCGATGAGCGTCGGTATCCGCCTGCCGAGGGTGTGGGCGGTGGTCTGGGCGGTGGCCGGGTTTGTCGGGTTGGTGGCCGGGCTGCTCTGGGgcg                                                                                                                                     >  2:79069/1‑149 (MQ=255)
            ccGCACGCGCATCGGCCTGGCGCTGCGTGCGGTGGCCGACGATCCCCGCGCGGCGATGAGCGTCGGTATCCGCCTGCCGAGGGTGTGGGCGGTGGTCTGGGCGGTGGCCGGGTTTGTCGGGTTGGTGGCCGGGCTGCTCTGGGGCGCAc                                                                                                                                  >  7:69587/1‑149 (MQ=255)
                               gcgcTGCGTGCGGTGGCCGACGATCCCCGCGCGGCGATGAGCGTCGGTATCCGCCTGCCGAGGGTGTGGGCGGTGGTCTGGGCGGTGGCCGGGTTTGTCGGGTTGGTGGCCGGGCTGCTCTGGGGCGCACGCCTGGGGGTGCAGTtctc                                                                                                               >  7:11649/1‑149 (MQ=255)
                               gcacTGCGTGCGGTGGCCGACGATCCCCGCGCGGCGATGAGCGTCGGTATCCGCCTGCCGAGGGTGTGGGCGCTGGTCTGGGCGGTGGCCGGGGTTGTCGGGTTGGTGGCCGGGCTGCTCTGGGGCGCACGCCTGGGGGTGCAGTtctc                                                                                                               >  5:158825/4‑149 (MQ=255)
                                      gtgcgGTGGCCGACGATCCCCGCGCGGCGATGAGCGTCGGTATCCGCCTGCCGAGGGTGTGGGCGGTGGTCTGGGCGGTGGCCGGGTTTGTCGGGTTGGTGGCCGGGCTGCTCTGGGGCGCACGCCTGGGGGTGCAGTtctcgctctcg                                                                                                        >  7:128449/1‑149 (MQ=255)
                                       tgcgGTGGCCGACGATCCCCGCGCGGCGATGAGCGTCGGTATCCGCCTGCCGAGGGTGTGGGCGGTGGTCTGGGCGGTGGCCGGGTTTGTCGGGTTGGTGGCCGGGCTGCTCTGGGGCGCACGCCTGGGGGTGCAGTtctcgctc                                                                                                           <  8:169108/145‑1 (MQ=255)
                                       tgcgGTGGCCGACGATCCCCGCGCGGCGATGAGCGTCGGTATCCGCCTGCCGAGGGTGTGGGCGGTGGTCTGGGCGGTGGCCGGGTTTGTCGGGTTGGTGGCCGGGCTGCTCTGGGGCGCACGCCTGGGGGTGCAGTtctcgctc                                                                                                           >  7:169108/1‑145 (MQ=255)
                                         cgGTGGCCGACGATCCCCGCGCGGCGATGAGCGTCGGTATCCGCCTGCCGAGGGTGTGGGCGGTGGTCTGGGCGGTGGCCGGGTTTGTCGGGTTGGTGGCCGGGCTGCTCTGGGGCGCACGCCTGGGGGTGCAGTTCTCGCTCTCGCtg                                                                                                     <  2:220310/149‑1 (MQ=255)
                                          ggTGGCCGACGATCCCCGCGCGGCGATGAGCGTCGGTATCCGCCTGCCGAGGGTGTGGGCGGTGGTCTGGGCGGTGGCCGGGTTTGTCGGGTTGGTGGCCGGGCTGCTCTGGGGCGCACGCCTGGGGGTGCAGTTCTCGCTCTCGCtgg                                                                                                    >  4:302200/1‑149 (MQ=255)
                                            tGGCCGACGATCCCCGCGCGGCGATGAGCGTCGGTATCCGCCTGCCGAGGGTGTGGGCGGTGGTCTGGGCGGTGGCCGGGTTTGTCGGGTTGGTGGCCGGGCTGCTCTGGGGCGCACGCCTGGGGGTGCAGTTCTCGCTCTCGCtggtg                                                                                                  <  8:88497/149‑1 (MQ=255)
                                            tGGCCGACGATCCCCGCGCGGCGATGAGCGTCGGTATCCGCCTGCCGAGGGTGTGGGCGGTGGTCTGGGCGGTGGCCGGGTTTGTCGGGTTGGTGGCCGGGCTGCTCTGGGGCGCACGCCTGGGGGTGCAGTTCTCGCTCTCGCtggtg                                                                                                  <  4:146788/149‑1 (MQ=255)
                                            tGGCCGACGATCCCCGCGCGGCGATGAGCGTCGGTATCCGCCTGCCGAGGGTGTGGGCGGTGGTCTGGGCGGTGGCCGGGTTTGTCGGGTTGGTGGCCGGGCTGCTCTGGGGCGCACGCCTGGGGGTGCAGTTCTCGCTCTCGCtggtg                                                                                                  <  8:69587/149‑1 (MQ=255)
                                            tGGCCGACGATCCCCGCGCGGCGATGAGCGTCGGTATCCGCCTGCCGAGGGTGTGGGCGGTGGTCTGGGCGGTGGCCGGGTTTGTCGGGTTGGTGGCCGGGCTGCTCTGGGGCGCACGCCTGGGGGTGCAGTTCTCGCTCTCGCtggtg                                                                                                  <  8:83945/149‑1 (MQ=255)
                                                cgacgaTCCCCGCGCGGCGATGAGCGTCGGTATCCGCCTGCCGAGGGTGTGGGCGGTGGTCTGGGCGGTGGCCGGGTTTGTCGGGTTGGTGGCTGGGCTGCTCTGGGGCGCACGCCTGGGGGTGCAGTTCTCGCTCTCGCTGGTGGTGc                                                                                              >  6:31963/1‑149 (MQ=255)
                                                cgacgaTCCCCGCGCGGCGATGAGCGTCGGTATCCGAATGCCGAGGGTGTGGGCGGTGGTCTGGGCGGTGGCCGGGTTTGTCGGGTTGGTGGCTGGGCTGCTCTGGGGCGCACGCCTGGGGGTGCAGTTCTCGCTCTCGCTGGTGGTGc                                                                                              >  6:27105/1‑149 (MQ=255)
                                                    gaTCCCCGCGCGGCGATGAGCGTCGGTATCCGCCTGCCGAGGGTGTGGGCGGTGGTCTGGGCGGTGGCCGGGTTTGTCGGGTTGGTGGCCGGGCTGCTCTGGGGCGCACGCCTGGGGGTGCAGTTCTCGCTCTCGCtggtgg                                                                                                 <  2:51266/142‑1 (MQ=255)
                                                    gaTCCCCGCGCGGCGATGAGCGTCGGTATCCGCCTACCGAGGGTGTGGGCGGTGGTCTGGGCGGTGGCCGGGTTTGTCGGGTTGGTGGCCGGGCTGCTCTGGGGCGCACGCCTGGGGGTGCAGTTCTCGCTCTCGCtggtgg                                                                                                 >  1:51266/1‑142 (MQ=255)
                                                      tCCCCGCGCGGCGATGAGCGTCGGTATCCGCCTGCCGAGGGTGTGGGCGGTGGTCTGGGCGGTGGCCGGGTTTGTCGGGTTGGTGGCCGGGCTGCTCTGGGGCGCACGCCTGGGGGTGCAGTTCTCGCTCTCGCTGGTGGTGCTCAAgg                                                                                        <  1:79069/149‑1 (MQ=255)
                                                         ccGCGCGGCGATGAGCGTCGGTATCCGCCTGCCGAGGGTGTGGGCGGTGGTCTGGGCGGTGGCCGGGTTTGTCGGGTTGGTGGCCGGGCTGCTCTGGGGCGCACGCCTGGGGGTGCAGTTCTCGCTCTCGCTGGTGGTGCTCAAGGccc                                                                                     >  8:105755/1‑149 (MQ=255)
                                                         ccGCGCGGCGATGAGCGTCGGTATCCGACTGCCGAGTGTGTTGGCGGTGGTCTGGGCGGTTGCCGGGTTTGTCGGGTTGGTGGCCGGGCTGCTCTGGGGCGCACGCCTGGGGGTGCAGTTCTCGCTCTCGCTGGTGGTGCTCAAGGccc                                                                                     >  6:90935/1‑149 (MQ=255)
                                                            cgcgGCGATGAGCGTCGGTATCCGCCTGCCGAGGGTGTGGGCGGTGGTCTGGGCGGTGGCCGGGTTTGTCGGGTTGGTGGCCGGGCTGCTCTGGGGCGCACGCCTGGGGGTGCAGTTCTCGCTCTCGCTGGTGGTGCTCAAGGCCCTGc                                                                                  <  7:105755/149‑1 (MQ=255)
                                                                             gTATCCGCCTGCCGTGGGTGTGGGCGGTGGGCTGGGCGGTGGCCGGGTTTGTCGGGTTGGTGGCCGGGCTGCTCTGGGGCGCACGCCTGGGGGTGCAGTTCTCGCTCTCGCTGGTGGTGCTCAAGGCGCTGCCGGTGCTGATCATcggc                                                                 <  6:158825/149‑1 (MQ=255)
                                                                              tATCCGCCTGCCGAGGGTGTGGGCGGTGGTCTGGGCGGTGGCCGGGTTTGTCGGGTTGGTGGCCGGGCTGCTCTGGGGCGCACGCCTGGGGGTGCAGTTCTCGCTCTCGCTGGTGGTGCTCAAGGCCCTGCCGGTGCTGATCATcggcg                                                                <  1:26325/149‑1 (MQ=255)
                                                                                  cGCCTGCCGAGGGTGTGGGCGGTGGTCTGGGCGGTGGCCGGGTTTGTCGGGTTGGTGGCCGGGCTGCTCTGGGGCGCACGCCTGGGGGTGCAGTTCTCGCTCTCGCTGGTGGTGCTCAAGGCCCTGCCGGTGCTGATCATCGGCGGttt                                                            <  8:128449/149‑1 (MQ=255)
                                                                                  cGCCTGCCGAGGGTGTGGGCGGTGGTCTGGGCGGTGGCCGGGTTTGTCGGGTTGGTGGCCGGGCTGCTCTGGGGCGCACGCCTGGGGGTGCAGTTCTCGCTCTCGCTGGTGGTGCTCAAGGCCCTGCCGGTGCTGATCATCGGCGGttt                                                            >  7:99723/1‑149 (MQ=255)
                                                                                   gCCTGCCGAGGGTGTGGGCGGTGGTCTGGGCGGTGGCCGGGTTTGTCGGGTTGGTGGCCGGGCTGCTCTGGGGCGCACGCCTGGGGGTGCAGTTCTCGCTCTCGCTGGTGGTGCTCAAGGCCCTGCCGGTGCTGATCATCGGCGGtttt                                                           <  2:318867/149‑1 (MQ=255)
                                                                                          gAGGGTGTGGGCGGTGGTCTGGGCGGTGGCCGGGTTTGTCGGGTTGGTGGCCGGGCTGCTCTGGGGCGCACGCCTGGGGGTGCAGTTCTCGCTCTCGCTGGTGGTGCTCAAGGCCCTGCCGGTGCTGATCATCGGCGGTTTTACCTCGa                                                    >  5:119487/1‑149 (MQ=255)
                                                                                                          gtCTGGGCGGTGGCCGGGTTTGTCGGGTTGGTGGCCGGGCTGCTCTGGGGCGCACGCCTGGGGGTGCAGTTCTCGCTCTCGCTGGTGGTGCTCAAGGCCCTGCCGGTGCTGATCATCGGCGGTTTTACCTCGATCAGCGGGGCGAtcgt                                    >  6:109979/1‑149 (MQ=255)
                                                                                                              gggCGGTGGCCGGGTTTGTCGGGTTGGTGGCCGGGCTGCTCTGGGGCGCACACCTGGGGGTGCAGTTCTCGCTCTCGCTGGTGGTGCTCAAGGCCCTGCCGGTGCTGATCATCGGCGGTTTTACCTCGATCAGCGGGGCGATCGTcggc                                >  7:78521/1‑149 (MQ=255)
                                                                                                                gCGGTGGCCGGGTTTGTCGGGTTGGTGGCTGGGCTGCTCTGGGGCGCACGCCTGGGGGTGCAGTTCTCGCTCTCGCTGGTGGTGCTCAAGGCCCTGCCGGTGCTGATCATCGGCGGTTTTACCTCGATCAGCGGGGCGATCGTcggcgg                              <  5:27105/149‑1 (MQ=255)
                                                                                                                gCGGTGGCCGGGTTTGTCGGGTTGGTGGCTGGGCTGCTCTGGGGCGCACGCCTGGGGGTGCAGTTCTCGCTCTCGCTGGTGGTGCTCAAGGCCCTGCCGGTGCTGATCATCGGCGGTTTTACCTCGATCAGCGGGGCGATCGTcggcgg                              <  5:31963/149‑1 (MQ=255)
                                                                                                                gCGGTGGCCGGGTTTGTCGGGTTGGCGGCCGGGCTGCTCTGGGGCGCACGCCTGGGGGTGCAGTTCTCGCTCTCGCTTGTGGTGCTCAAGGCCCTGCCGGTGCTGATCATCGGCGGTTTTACCTCGATCAGCGGGGCGATCGTcggcgg                              <  7:209557/149‑1 (MQ=255)
                                                                                                                       ccGGGTTTGTCGGGTTGGTGGCCGGGCTGCTCTGGGGCGCACGCCTGGGGGTGCAGTTCTCGCTCTCGCTGGTGGTGCTCAAGGCCCTGCCGGTGCTGATCATCGGCGGTTTTACCTCGATCAGCgggg                                           >  4:121045/1‑129 (MQ=255)
                                                                                                                       ccGGGTTTGTCGGGTTGGTGGCCGGGCTGCTCTGGGGCGCACGCCTGGGGGTGCAGTTCTCGCTCTCGCTGGTGGTGCTCAAGGCCCTGCCGGTGCTGATCATCGGCGGTTTTACCTCGATCAGCgggg                                           <  3:121045/129‑1 (MQ=255)
                                                                                                                       ccGGGTTTGTCGGGTTGGTGGCCGGGCTGCTCTGGGGCGCACGCCTGGGGGTGCAGTTCTCGCTCTCGCTGGTGGTGCTCAAGGCCCTGCCGGTGCTGATCATCGGCGGTTTTACCTCGATCAGCGGGGCGATCGTCGGCGGCCTGatc                       <  8:11649/149‑1 (MQ=255)
                                                                                                                        cGGGTTTGTCGGGTTGGTGGCCGGGCTGCTCTGGGGCGCACGCCTGGGGGTGCAGTTCTCGCTCTCGCTGGTGGTGCTCAAGGCCCTGCCGGTGCTGATCATCGGCGGTTTTACCTCGATCAGCGGGGCGATCGTCGGCGGCCTGatca                      >  4:43328/1‑149 (MQ=255)
                                                                                                                         gggTTTGTCGGGTTGGTGGCCGGGCTGCTCTGGGGCGCACGCCTGGGGGTGCAGTTCTCGCTCTCGCTGGTGGTGCTCAAGGCCCTGCCGGTGCTGATCATCGGCGGTTTTACCTCGATCAGCGGGGCGATCGTCGGCGGCCTGatcat                     <  3:302200/149‑1 (MQ=255)
                                                                                                                          ggTTTGTCGGGGTGGTGGCCGGGCTGCTCTGGGGCGCACGCCTGGGGGTGCAGTTCTCGCTCTCGCTGGTGGTGCTCAAGGCCCTGCCGGTGCTGATCATCGGCGGTTTTACCTCGATCAGCGGGGCGATCGTCGGCGGCCTGatcatc                    <  5:90935/149‑1 (MQ=255)
                                                                                                                            tttGTCGGGTTGGTGGCCGGGCTGCTCTGGGGCGCACGCCTGGGGGTGCAGTTCTCGCTCTCGCTGGTGGTGCTCAAGGCCCTGCCGGTGCTGATCATCGGCGGTTTTACCTCGATCAGCGGGGCGATCGTCGGCGGCCTGATCATCgg                  >  5:64411/1‑149 (MQ=255)
                                                                                                                                 cGGGTTGGTGGCCGGGCTGCTCTGGGGCGCACGCCTGGGGGTGCAGTTCTCGCTCTCGCTGGTGGTGCTCAAGGCCCTGCCGGTGCTGATCATCGGCGGTTTTACCTCGATCAGCGGGGCGATCGTCGGCGGCCTGATCATCGGCGCtt             >  3:104588/1‑149 (MQ=255)
                                                                                                                                  gggTTGGTGGTCGGGCTGCTCTGGGGCGCACGCCTGGGGGTGCAGTTCTCGCTCTCGCTGGTGGTGCTCAAGGCCCTGCCGGTGCTGATCATCGGCGGTTTTACCTCGATCAGCGGGGCGATCGTCGGCGGCCTGATCATCGGCGCTTc            >  3:49454/1‑149 (MQ=255)
                                                                                                                                            ccGGGCTGCTCTGGGGCGCACGCCTGGGGGTGCAGTTCTCGCTCTCGCTGGTGGTGCTCAAGGCCCTGCCGGTGCTGATCATCGGCGGTTTTACCTCGATCAGCGGGGCGATCGTCGGCGGCCTGATCATCGGCGCTTCGGAGAAGCTc  >  8:314191/1‑149 (MQ=255)
                                                                                                                                             |                                                                                                                                                   
CTTGCTGTTCAACCGCACGCGCATCGGCCTGGCGCTGCGTGCGGTGGCCGACGATCCCCGCGCGGCGATGAGCGTCGGTATCCGCCTGCCGAGGGTGTGGGCGGTGGTCTGGGCGGTGGCCGGGTTTGTCGGGTTGGTGGCCGGGCTGCTCTGGGGCGCACGCCTGGGGGTGCAGTTCTCGCTCTCGCTGGTGGTGCTCAAGGCCCTGCCGGTGCTGATCATCGGCGGTTTTACCTCGATCAGCGGGGCGATCGTCGGCGGCCTGATCATCGGCGCTTCGGAGAAGCTC  >  NC_002947/3152310‑3152598

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑