| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 3,198,910 | A→T | 15.1% | intergenic (‑254/+23) | ethA ← / ← PP_2806 | FAD‑containing monooxygenase EthA/TetR family transcriptional regulator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 3,198,910 | 0 | A | T | 15.1% | 77.7 / 6.7 | 33 | intergenic (‑254/+23) | ethA/PP_2806 | FAD‑containing monooxygenase EthA/TetR family transcriptional regulator |
| Reads supporting (aligned to +/- strand): ref base A (20/8); new base T (3/2); total (23/10) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.27e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.29e-01 | |||||||||||
ACTGCTATTTCAGGATGATTTGCACCTGTTACTTCGCCGATCAACGATCAGACTGGGTCAGTCTGGTAGATATCGTCATCGGAACGTTCGAAGGGGGGCGCTGCGGCTTTCAGAGCCGGAGGGGGAGGGGATCGCCGTGCAGCGACGCAGCACGGCGAATCCTGTGGGGCCTCAGTAACTGGCCACCAGCAACTTGACTGCCTGGCAGGCGGTCTCGGTCTCGGCCTCCAGGCGTGCACGGTCGCCGTGAGCGGACCAGGCGAGCATCAGGCCGTCGAACAGATTGAT > NC_002947/3198762‑3199049 | aCTGCTATTTCAGGATGATTTGCACCTGTTACTTCGCCGATCAACGATCAGACTGGGTCAGTCTGGTAGATATCGTCATCGGAACGTTCGAAGGGGGGCGCTGCGGCTTTCAGAGCCGGAGGGGGAGGGGATCGCCGTGCAGCGACgca > 3:4812/1‑149 (MQ=255) aTTTCAGGATGATTTGCACCTGTTACTTCGCCGATCAACGATCAGACTGGGTCAGTCTGGTAGATATCGTCATCGGAACGTTCGAAGGGGGGCGCTGCGGCTTTCAGAGCCGGAGGGGGAGGGGATCGCCGTGCAGCGACGCAGCACgg > 7:52361/1‑149 (MQ=255) tttGCGCCTGTTACTTCGCCGATCAACGATCAGACTGGGTCAGTCTGGTAGATATCGTCATCGGAACGTTCGAAGGGGGGCGCTGCGGCTTTCAGAGCCGGAGGGGGAGGGGATCGCCGTGCAGCGACCCAGCACGGCGAATCCTGTgg > 7:302698/1‑149 (MQ=255) tttGCACCTGTTACTTCGCCGATCAACGATCAGACTGGGTCAGTCTGGTAGATATCGTCATCGGAACGTTCGAAGGGGGGCGCTGCGGCTTTCAGAGCCGGATGGGGAGGGGATCGCCGTGCAGCGACGCAGCACGGCGAATCCTGTgg > 7:134216/1‑149 (MQ=255) ttGCACCTGTTACTTCGCCGATCAACGATCAGACTGGGTCAGTCTGGTAGATATCGTCATCGGAACGTTCGAAGGGGGGCGCTGCGGCTTTCAGAGCCGGAGGGGGAGGGGATCGCCGTGCAGCGACGCAGCACGGCGAATCCTGTggg > 5:35443/1‑149 (MQ=255) ttGCACCTGTTACTTCGCCGATCAACGATCAGACTGGGTCAGTCTGGTAGATATCGTCATCGGAACGTTCGAAGGGGGGCGCTGCGGCTTTCAGAGCCGGAGGGGGAGGGGATCGCCGTGCAGCGACGCAGCACGGCGAATCCTGTggg > 3:76480/1‑149 (MQ=255) cTTCGCCGATCAACGATCAGACTGGGTCAGTCTGGTAGATATCGTCATCGGAACGTTCGAAGGGCGGCGATGCGGCTTTCAGAGCCGGAGGGGGAGGGGATCGCCGTGCAGCGACGCACCACGGCGAATCCTCTGGGGCCTCAGTAACt > 7:317298/1‑149 (MQ=255) accAGACTGACCCAGTCTGGTAGATATCGTCATCGGAACGTTCGAAGGGGGGCGCTGCGGCTTTCAGAGCCGGAGGGGGAGGGGATCGCCGTGCAGCGACGCAGCACGGCGAATCCTGTGGGGCCTCAGTAACTGGCCACCAGCAACtt > 7:222571/3‑149 (MQ=255) gACTGGGTCAGTCTGGTAGATATCGTCATCGGAACGTTCGAAGGGGGGCGCTGCGGCTTTCAGAGCCGGAGGGGGAGGGGATCGCCGTGCAGCGACGCAGCACGGCGAATCCTGTGGGGCCTCAGTAACTGGCCACCAGCAACTTGACt > 5:100465/1‑149 (MQ=255) gACTGGGTCAGTCTGGTAGATATCGTCATCGGAACGTTCGAAGGGGGGCGCTGCGGCTTTCAGAGCCGGAGGGGGAGGGGATCGCCGTGCAGCGACGCAGCACGGCGAATCCTGTGGGGCCTCAGTAACTGGCCACCAGCAACTTGACt > 7:39427/1‑149 (MQ=255) gggTCAGTCTTTTATATTTCTTCATCGTAACTTTCGCTGGGGGGCGCTGCGGATTTCAGAGCCGGATGGGGTGGGGATCGCCGTGCAGCGACGCAGCACGGCGAATCCTGTGGGGCCTCAGTAACTGGCCACCAGCAACTTGACTGcc < 8:134216/148‑1 (MQ=255) tCTGGTAGATATCGTCATCGGAACGTTCGAAGGGGGGCGCTGCGGCTTTCAGAGCCGGAGGGGGAGGGGATCGCCGTGCAGCGACGCAGCACGGCGAATCCTGTGGGGCCTCAGTAACTGGCCACCAGCAACTTGACTGCCTGGCAGGc > 4:121768/1‑149 (MQ=255) gTAGATATCGTCCTCGGAACGTTCGCCGGGGGGCGCTGCGGCTTTCCGAGCCGGAGGGGGCGGGGATCGCCGTGCAGCGACGCAGCACGGCGAATCCTGTGGGGCCTCAGTAACTGGCCACCAGCAACTTGACTGCCTGGCAGGcggtc < 6:168515/149‑1 (MQ=255) gTAGATATCGTCATCGGAACGTTCGAAGGGGGGCGCTGCGGCTTTCAGAGCCGGAGGGGGAGGGGATCGCCGTGCAGCGACGCAGCACGGCGAATCCTGTGGGGCCTCAGTAACTGGCCACCAGCAACTTGACTGCCTGGCAGGcggtc > 1:25633/1‑149 (MQ=255) aGATATCGTCATCGGAACGTTCGAAGGGGGGCGCTGCGGCTTTCAGAGCCGGAGGGGGAGGGGATCGCCGTGCAGCGACGCAGCACGGCGAATCCTGTGGGGCCTCAGTAACTGGCCACCAGCAACTTGACTGCCTGGCAGGcggtctc > 2:60941/1‑149 (MQ=255) aTCGGAACGTTCGAAGGGGGGCGCTGCGGCTTTCAGAGCCGGAGGGGGAGGGGATCGCCGTGCAGCGACGCAGCACGGCGAATCCTGTGGGGCCTCAGTAACt > 3:263406/1‑103 (MQ=255) aaCGTTCGAAGGGGGGCGCTGCGGCTTTCAGAGCCGGAGGGGGAGGGGATCGCCGTGCAGCGACGCAGCACGGCGAATCCTGTGGGGCCTCAGTAACTGGCCACCAGCAACTTGCCTGCCTGGCAGGCGGTCTCGGTCTCGGCCTCCAg > 8:309839/1‑149 (MQ=255) cTTTCAGAGCCGGAGGGGGAGGGGATCGCTGTGCAGCGACGCAGCACGGCGAATCCTGTGGGGCCTCAGTAACTGGCCACAAGCAACTTGACTGCCTGCCAGGCGGTCTCGGTCTCGGCCTCCAGGCGTGCACGGTCGCCGt > 7:275441/1‑142 (MQ=255) cTTTAAGAGGCGGAGGGGGAGGGGATCGCTGTGAAGCGACGCAGCACGGAGAATCCTGTGGGGCCTCAGTAACTGGCCACCAGCAACTTGACTGCCTGGCAGGCGGTCTCGGTCTCGGCCTCCAGGCGTGCACGGTCGCCGt < 8:275441/142‑1 (MQ=255) tttCAGAGCCGGAGGGGGAGGGGATCGCCGTGCAGCGACGCAGCACGGCGAATCCTGTGGGGCCTCAGTAACTGGCCACCAGCAACTTGACTGCCTGGCAGGCGGTCTCGGTCTCGGCCTCCAGGCGTGCACGGTCGCCGTGAGCGGAc > 8:88624/1‑149 (MQ=255) cAGCGCCGGCGGGGGCGGGGATCGCCGTGCAGCGACGCAGCACGGCGAATCCTGTGGGGCCTCAGTAACTGGCCACCAGCAACTTGACTGCCTGGCAGGCGGTCTCGGTCTCGGCCTCCAGGCGTGCACGGTCGCCGTGAGCGGACCa < 8:61071/148‑1 (MQ=255) agCCGGAGGGGGAGGGGATCGCCGTGCAGCGACGCAGCACGGCGAATCCTGTGGGGCGTACGTAAATGGCCACCAGCAAATTGAATCCATGGCAGCCGGTCACGGTCGCGGCCTCCAGCCGTCCACGGACGCCGTGACCGCCCCAGGCg > 8:67486/1‑149 (MQ=255) cGGAGGGGGAGGGGATCGCCGTGCAGCGACGCAGCACGGCGAATCCTGTGGGGCCTCAGTAACTGGCCACCAGCAACTTGACTGCCTGGCAGGCGGTCTCGGTCTCGGCCTCCAGGCGTGCACGGTCGCCGTGAGCGGACCAg > 7:239322/1‑143 (MQ=255) cGGAGGGGGAGGGGATCGCCGTGCAGCGACGCAGCACGGCGAATCCTGTGGGGCCTCAGTAACTGGCCACCAGCAACTTGACTGCCTGGCAGGCGGTCTCGGTCTCGGCCTCCAGGCGTGCACGGTCGCCGTGAGCGGACCAg < 8:239322/143‑1 (MQ=255) ggggaggggaTCGCCGTGCAGCGACGCAGCACGGCGAATCCTGTGGGGCCTCAGTAACTGGCCACCAGCAACTTGACTGCCTGGCAGGCGGTCTCGGTCTCGGCCTCCAGGCGTGCACGGTCGCCGTGAGCGGACCAGGCGAGCATCAg < 8:52361/149‑1 (MQ=255) attCGCCGTGCTGCGTCGCTGCACGGCGAATCCTGTGGGGCCTCAGTAACTGGCCACCAGCAACTTGACTGCCTGGCAGGCGGTCTCGGTCTCGGCCTCCAGGCGt < 1:58176/104‑1 (MQ=39) attCGCCGTGCTGCGTCGCTGCACGGCGAATCCTGTGGGGCCTCAGTAACTGGCCACCAGCAACTTGACTGCCTGGCAGGCGGTCTCGGTCTCGGCCTCCAGGCGt > 2:58176/3‑106 (MQ=39) cGCCGTGCTGCGTCGCTGCACGGCGAATCCTGTGGGGCCTCAGTAACTGGCCACCAGCAACTTGACTGCCTGGCAGGCGGTCTCGGTCTCGGCCTCCAGGCGTGCACGGTCGCCGTGAGCGGACCAGGCGAGCATCAGGCCGTCGaa > 7:188418/1‑147 (MQ=255) cGCCGTGCAGCGACGCAGCACGGCGAATCCTGTGGGGCCTCAGTAACTGGCCACCAGCAACTTGACTGCCTGGCAGGCGGTCTCGGTCTCGGCCTCCAGGCGTGCACGGTCGCCGTGAGCGGACCAGGCGAGCATCAGGCCGTCGAAc < 8:222571/148‑1 (MQ=255) gCCGTGCTGCGTCGCTGCACGGCGAATCCTGTGGGGCCTCAGTAACTGGCCACCAGCAACTTGACTGCCTGGCAGGCGGTCTCGGTCTCGGCCTCCAGGCGTGCACGGTCGCCGTGAGc > 8:136100/1‑119 (MQ=255) gCCGTGCTGCGTCGCTGCACGGCGAATCCTGTGGGGCCTCAGTAACTGGCCACCAGCAACTTGACTGCCTGGCAGGCGGTCTCGGTCTCGGCCTCCAGGCGTGCACGGTCGCCGTGAGc < 7:136100/119‑1 (MQ=255) aGCGACGCAGCACGGCGAATCCTGTGGGGCCTCAGTAACTGGCCACCAGCAACTTGACTGCCTGGCAGGCGGTCTCGGTCTCGGCCTCCAGGCGTGCACGGTCGCCGTGAGCGGACCAGGCGAGCATCAGGCCGTCGAACAGATTGAt > 5:214740/1‑148 (MQ=255) cGACGCAGCACGGCGAATCCTGTGGGGCCTCAGTAACTGGCCACCAGCAACTTGACTGCCTGGCAGGCGGTCTCGGTCTCGGCCTCCAGGCGTGCACGGTCGCCGTGAGCGGACCAGGCGAGCATCAGGCCGTCGa > 8:242876/1‑136 (MQ=255) cGACGCAGCACGGCGAATCCTGTGGGGCCTCAGTAACTGGCCACCAGCAACTTGACTGCCTGGCAGGCGGTCTCGGTCTCGGCCTCCAGGCGTGCACGGTCGCCGTGAGCGGACCAGGCGAGCATCAGGCCGTCGa < 7:242876/136‑1 (MQ=255) | ACTGCTATTTCAGGATGATTTGCACCTGTTACTTCGCCGATCAACGATCAGACTGGGTCAGTCTGGTAGATATCGTCATCGGAACGTTCGAAGGGGGGCGCTGCGGCTTTCAGAGCCGGAGGGGGAGGGGATCGCCGTGCAGCGACGCAGCACGGCGAATCCTGTGGGGCCTCAGTAACTGGCCACCAGCAACTTGACTGCCTGGCAGGCGGTCTCGGTCTCGGCCTCCAGGCGTGCACGGTCGCCGTGAGCGGACCAGGCGAGCATCAGGCCGTCGAACAGATTGAT > NC_002947/3198762‑3199049 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |