Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,208,940 T→A 8.9% V55E (GTG→GAG)  PP_2813 → BNR domain‑containing protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,208,9400TA 8.9% 145.6 / 2.0 52V55E (GTG→GAG) PP_2813BNR domain‑containing protein
Reads supporting (aligned to +/- strand):  ref base T (19/28);  new base A (2/3);  total (21/31)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ATTCACCGCGGCCTTGCTCGCAGCAGCATTGCCGCTGATGTCTCTTCAGGCCGCGCCGTTGCAGGCCGTGCCGGCCGTACAGGCGAGCAATGCCACGCAGGCCACCATGTTGGCCGCTGGCTGGGCCGGTGCGCGCGTGGTTGC‑CGTGGGCGACCACGGTGTCGTGTTGTTGTCCGACGACCAGGGCCTGCAGTGGCGCCAGGCGAGTTCGGTACCGCTGTCCACACCATTGACGGGCGTGTCTTTCGTGGATGCCAAACGCGGGTGGGCGGTTGGCCACTGGGGCGCGATCC  >  NC_002947/3208794‑3209086
                                                                                                                                                   |                                                                                                                                                  
attcaCCGCGGCCTTGCTCGCAGCAGCATTGCCGCTGATGTCTCTTCAGGCCGCGCCGTTGCAGGCCGTGCCGGCCGTACAGGCGAGCAATGCCACGCAGGCCACCATGTTGGCCGCTGGCTGGGCCGGTGCGCGCGTGGTTGC‑CGTgg                                                                                                                                                  <  5:92014/149‑1 (MQ=255)
atgcaCCGCGGCCTTGCTCGCAGCAGCATTGCCGCTGATGTCTCTTCAGGCCGCGCCGTTGCAGGCCGTGCCGGCCGTACAGGCGAGCAATGCCACGCAGGCCACCATGTTGGCCGCTGGCTGGGCCGGTGCGCGCGTGGTTGC‑CGTgg                                                                                                                                                  <  8:148226/146‑1 (MQ=255)
 ttcaCCGCGGCCTTGCTCGCAGCAGCATTGCCGCTGATGTCTCTTCAGGCCGCGCCGTTGCAGGCCGTGCCGGCCGTACAGGCGAGCAATGCCACGCAGGCCACCATGTTGGCCGCTGGCTGGGCCGGTGCGCGCGTGGTTGC‑CGTggg                                                                                                                                                 <  6:80699/149‑1 (MQ=255)
 ttcaCCGCGGCCTTGCTCGCAGCAGCATTGCCGCTGATGTCTCTTCAGGCCGCGCCGTTGCAGGCCGTGCCGGCCGTACAGGCGAGCAATGCCACGCAGGCCACCATGTTGGCCGCTGGCTGGGCCGGTGCGCGCGTGGTTGC‑CGTggg                                                                                                                                                 <  1:126953/149‑1 (MQ=255)
   caCCGCGGCCTTGCTCGCAGCAGCATTGCCGCTGATGTCTCTTCAGGCCGCGCCGTTGCAGGCCGTGCCGGCCGTACAGGCGAGCAATGCCACGCAGGCCACCATGTTGGCCGCTGGCTGGGCCGGTGCGCGCGTGGTTGC‑CGTGGGc                                                                                                                                                <  6:252547/148‑1 (MQ=255)
     ccGCGGCCTTGCTCGCAGCAGCATTGCCGCTGATGTCTCTTCAGGCCGCGCCGTTGCAGGCCGTGCCGGCCGTACAGGCGAGCAATGCCACGCAGGCCACCATGTTGGCCGCTGGCTGGGCCGGTGCGCGCGTGGTTGC‑CGTGGGCGAc                                                                                                                                             >  2:53390/1‑149 (MQ=255)
        cgGCCTTGCTCGCAGCAGCATTGCCGCTGATGTCTCTTCAGGCCGCGCCGTTGCAGGCCGTGCCGGCCGTACAGGCGAGCAATGCCACGCAGGCCACCATGTTGGCCGCTGGCTGGGCCGGTGCGCGCGTGGTCGC‑CCACGGCAACCAc                                                                                                                                          <  4:287694/149‑1 (MQ=255)
            cTTGCTCGCAGCAGCATTGCCGCTGATGTCTCTTCAGGCCGCGCCGTTGCAGGCCGTGCCGGCCGTACAGGCGAGCAATGCCACGCAGGCCACCATGTTGGCCGCTGGCTGGGCCGGTGCGCGCGTGGTTGC‑CGTGGGCGACCACGgtg                                                                                                                                      >  8:43594/1‑149 (MQ=255)
                cTCGCAGCAGCATTGCCGCTGATGTCTCTTCAGGCCGCGCCGTTGCAGGCCGTGCCGGCCGTACAGGCGAGCAATGCCACGCAGGCCACCATGTTGGCCGCTGGCTGGGCCGGTGCGCGCGTGGTTGC‑CGTGGGCGACCACGGTGTCgt                                                                                                                                  <  8:39592/149‑1 (MQ=255)
                   gcagcagcaTTGGCGCTGATGTCTCTTCAGGCCGCGCCGTTGCATGCCGTGCCGTCCGTAAAGGCGAGCAATGCCACGCAGGCCTCCTTGTTGGCCGCTGGCTGGGCCGGTGCGCGCGTGGTTGC‑CGTGGGCGACCACGGTGTCGtgtt                                                                                                                               <  5:10298/149‑1 (MQ=255)
                   gcagcagcaTTGCCGCTGATGTCTCTTCAGGCCGCGCCGTTGCAGGCCGTGCCGGCCGTACAGGCGAGCAATGCCACGCAGGCCACCATGTTGGCCGCTGGCTGGGCCGGTGCGCGCGTGGTTGC‑CGTGGGCGACCACGGTGTCgtgt                                                                                                                                <  1:53390/148‑1 (MQ=255)
                    cagcagcaTTGCCGCTGATGTCTCTTCAGGCCGCGCCGTTGCAGGCCGTGCCGGCCGTACAGGCGAGCAATGCCACGCAGGCCACCATGTTGGCCGCTGGCTGGGCCGGTGCGCGCGTGGTTGC‑CGTGGGCGACCACGGTGTCGtgttg                                                                                                                              <  5:150543/149‑1 (MQ=255)
                      gcagcaTTGCCGCTGATGTCTCTTCAGGCCGCGCCGTTGCAGGCCGTGCCGGCCGTACAGGCGAGCAATGCCACGCAGGCCACCATGTTGGCCGCTGGCTGGGCCGGTGCGCGCGTGGTTGC‑CGTGGGCGACCACGGTGTCGtgttgtt                                                                                                                            <  4:302953/149‑1 (MQ=255)
                        agcaTTGCCGCTGATGTCTCTTCAGGCCGCGCCGTTGCAGGCCGTGCCGGCCGTACAGGCGAGCAATGCCACGCAGGCCACCATGTTGGCCGCTGGCTGGGCCGGTGCGCGCGTGGTTGC‑CGTGGGCGACCACGGTGTCGtgttgttgt                                                                                                                          <  6:76457/149‑1 (MQ=255)
                                 gCTGATGTCTCTTCAGGCCGCGCCGTTGCAGGCCGTGCCGGCCGTACAGGCGAGCAATGCCACGCAGGCCACCATGTTGGCCGCTGGCTGGGCCGGTGCGCGCGTGGTTGC‑CGTGGGCGACCACGGTGTCGTGTTGTTGTCCGACGAcc                                                                                                                 <  5:143564/149‑1 (MQ=255)
                                     aTGTCTCTTCAGGCCGCGCCGTTGCAGGCCGTGCCGGCCGTACATGCGAGCAATGCCACGCAGGCCACCATGATGCCCGCTGGCTGGGCCGGTGCGCGCGTGGTTGC‑CGAGGGCGACCACGGTGTCGTGTGGTAGTCGGACGACCAggg                                                                                                             >  8:218158/1‑149 (MQ=255)
                                     aTGTCTCTTCAGGCCGCGCCGTTGCAGGCCGTGCCGGCCGTACAGGCGAGCAATGCCACGCAGGCCACCATGTTGGCCGCTGGCTGGGCCGGTGCGCGCGTGGTTGC‑CGTGGGCGACCACGGTGTCGTGTTGTTGTCCGACGACCAggg                                                                                                             >  3:287565/1‑149 (MQ=255)
                                      tGTCTCTTCAGGCCGCGCCGTTGCAGGCCGTGCCGGCCGTACAGGCGAGCAATGCCACGCAGGCCACCATGTTGGCCGCTGGCTGGGCCGGTGCGCGCGTGGTTGC‑CGTGGGCGACCACGGTGTCGTGTTGTTGTCCGACGACCAGGGc                                                                                                            <  4:287565/149‑1 (MQ=255)
                                           ctTCAGGCCGCGCCGTTGCAGGCCGTGCCGGCCGTACAGGCGAGCAATGCCACGCAGGCCACCATGTTGGCCGCTGGCTGGGCCGGTGCGCGCGTGGTCGC‑CCACGGCAACCACgc                                                                                                                                        <  6:250402/116‑2 (MQ=255)
                                           ctTCAGGCCGCGCCGTTGCAGGCCGTGCCGGCCGTACAGGCGAGCAATGCCACGCAGGCCACCATGTTGGCCGCTGGCTGGGCCGGTGCGCGCGTGGTCGC‑CCACGGCAACCACgc                                                                                                                                        >  5:250402/1‑115 (MQ=255)
                                            ttCAGGCCGCGCCGTTGCAGGCCGTGCCGGCCGTACAGGCGAGCAATGCCACGCAGGCCACCATGTTGGCCGCTGGCTGGGCCGGTGCGCGCGTGGTTGC‑CGTGGGCGACCACg                                                                                                                                         <  7:106617/114‑1 (MQ=255)
                                            ttCAGGCCGCGCCGTTGCAGGCCGTGCCGGCCGTACAGGCGAGCAATGCCACGCAGGCCACCATGTTGGCCGCTGGCTGGGCCGGTGCGCGCGTGGTTGC‑CGTGGGCGACCACg                                                                                                                                         >  8:106617/1‑114 (MQ=255)
                                            ttCAGGCCGCGCCGTTGCAGGCCGTGCCGGCCGTACAGGCGAGCAATGCCACGCAGGCCACCATGTTGGCCGCTGGCTGGGCCGGTGCGCGCGTGGTTGC‑CGTGGGCGACCACGGTGTCGTGTTGTTGTCCGACGACCAGGGCCTGCAg                                                                                                      >  7:27300/1‑149 (MQ=255)
                                                    gcgcCGTTGCAGGCCGTGCCGGCCGTACAGGCGAGCAATGCCACGCAGGCCACCATGTTGGCCGCTGGCTGGGCCGGTGCGCGCGTGGTTGC‑CGTGGGCGACCACGGTGTCGTGTTGTTGTCCGACGACCAGGGCCTGCAGTGGCGCCa                                                                                              <  3:36297/149‑1 (MQ=255)
                                                        cGTTGCAGGCCGTGCCGGCCGTACAGGCGAGCAATGCCACGCAGGCCACCATCTTGGCCTCTGGCTGGGCCGGTGCGCGCGTCGTTGC‑CGTGGGCGACCACGGTGTCCTCTTGTTGTCCGACGACCAGGGCCTGCAGTGGCGCCAGGc                                                                                           >  4:33474/1‑148 (MQ=255)
                                                              aGGCCGTGCCGGCCGGACAGGCGAGCAATGCCACGCAGGCCACCATGTGGGCCGCTGGCTGGGCCGGTGCGCGCGTGGGTGC‑CGTGGGCGACCACGGGGTCGTGTTGTTGTCCGACGACCAGGGCCTGCAGTGGCGCCAGGCGAGTTCg                                                                                    <  1:162197/149‑1 (MQ=255)
                                                                gCCGTGCCGGCCGTACAGGCGAGCAATGCCACGCAGGCCACCATGTTGGCCGCTGGCTGGGCCGGTGCGCGCGTGGTTGC‑CGTGGGCGACCACGGTGTCGTGTTGTTGTCCGACGACCAGGGCCTGCAGTGGCGCCAGGCGAGTTCgg                                                                                   >  6:18290/1‑148 (MQ=255)
                                                                    tGCCGGCCGTACAGGCGAGCAATGCCACGCAGGCCACCATGTTGGCCGCTGGCTGGGCCGGTGCGCGCGTGGTTGC‑CGTGGGCGACCACGGTGTCGTGTTGTTGTCCGACGACCAGGGCCTGCAGTGGCGCCAGGCGAGTTCGGTACCg                                                                              <  8:27300/149‑1 (MQ=255)
                                                                                aGGCGAGCAATGCCACGCAGGCCACCATGTTGGCCGCTGGCTGGGCCGGTGCGCGCGTGGTTGC‑CGTGGGCGACCACGGTGTCGTGTTGTTGTCCGACGACCAGGGCCTGCAGTGGCGCCAGGCGAGTTCGGTACCGCTGTCCACACCa                                                                  >  4:241640/1‑149 (MQ=255)
                                                                                  gCGAGCAATGCCACGCAGGCCACCATGTTGGCCGCTGGCTGGGCCGGTGCGCGCGTGGTTGC‑CGTGGGCGACCACGGTGTCGTGTTGTTGTCCGACGACCAGGGCCTGCAGTGGCGCCAGGc                                                                                           >  7:174058/1‑122 (MQ=255)
                                                                                  gCGAGCAATGCCACGCAGGCCACCATGTTGGCCGCTGGCTGGGCCGGTGCGCGCGTGGTTGC‑CGTGGGCGACCACGGTGTCGTGTTGTTGTCCGACGACCAGGGCCTGCAGTGGCGCCAGGc                                                                                           <  8:174058/122‑1 (MQ=255)
                                                                                            ccACGCAGGCCACCATGTTGGCCGCTGGCTGGGCCGGTGCGCGCGTGGTTGC‑CGTGGGCGACCACGGTGTCGTGTTGTTGTCCGACGACCAGGGCCTGCAGTGGCGCCAGGCGAGTTCGGTACCGCTGTCCACACCATTGACGGGCgt                                                       <  6:102505/148‑1 (MQ=255)
                                                                                              aCGCAGGCCACCATGTTGGCCGCTGGCTGGGCCGGTGCGCGCGTGGTTGC‑CGTGGGCGACCACGGTGTCGTGTTGTTGTCCGACGACCAGGGCCTGCAGTGGCGCCAGGCGAGTTCGGTACCGCTGTCCACACCATTGACGGGCGTGTc                                                    >  7:41794/1‑149 (MQ=255)
                                                                                                gCAGGCCACCATGTTGGCCGCTGGCTGGGCCGGTGCGCGCGTGGTTGC‑CGTGGGCGACCACGGTGTCGTGTTGTTGTCCGACGACCAGGGCCTGCAGTGGCGCCAGGCGAGTTCGGTACCGCTGTCCCCACCATTGACGGGCGTGTCtt                                                  >  4:186226/1‑149 (MQ=255)
                                                                                                    gCCACCATGTTGGCCGCTGGCTGGGCCGGTGCGCGCGTGGTTGC‑CGTGGGCGACCACGGTGTCGTGTTGTTGTCCGACGACCAGGGCCTGCAGTGGCGCCAGGCGAGTTCGGTACCGCTGTCCACACCATTGACGGGCGTGTCTTTCGt                                              <  5:18290/149‑1 (MQ=255)
                                                                                                     ccaccaTGTTGGCCGCTGGCTGGGCCGGTGCGCGCGTGGTTGCGCGTGGGCGACCACGGTGTCGTGTTGTTGTCCGACGACCAGGGCCTGCAGTGGCGCCAGGCGAGTTCGGTACCGCTGTCCACACCATTGACGGGCGTGTCTTTCGt                                              >  1:235946/1‑149 (MQ=255)
                                                                                                     ccaccaTGTTGGCCGCTGGCTGGGCCGGTGCGCGCGTGGTTGC‑CGTGGGCGACCACGGTGTCGTGTTGTTGTCCGACGACCAGGGCCTGCAGTGGCGCCAGGCGAGTTCGGTACCg                                                                              >  1:95093/1‑116 (MQ=255)
                                                                                                     ccaccaTGTTGGCCGCTGGCTGGGCCGGTGCGCGCGTGGTTGC‑CGTGGGCGACCACGGTGTCGTGTTGTTGTCCGACGACCAGGGCCTGCAGTGGCGCCAGGCGAGTTCGGTACCg                                                                              <  2:95093/116‑1 (MQ=255)
                                                                                                                  cGCTGGCTGGGCCGGTGCGCGCGTGGTTGC‑CGTGGGCGACCACGGTGTCGTGTTGTTGTCCGACGACCAGGGCCTGCAGTGGCGCCAGGCGAGTTCGGTACCGCTGTCCACACCATTGACGGGCGTGTCTTTCGTGGATGCCAAAcgcg                                >  2:85766/1‑149 (MQ=255)
                                                                                                                    ttggctggGCCGGTGCGCGCGTGGTTGC‑CGTGGGCGACCACGGTGTCGTGTTGTTGTCCGACGACCAGGGCCTGCAGTGGCGCCAGGCGAGTTCGGTACCGCTGTCCACACCATTGACGGGCGTGTCTTTCGTGGATGCCAAACGCggg                              <  3:114532/148‑1 (MQ=255)
                                                                                                                      ggctggGCCGGTGCGCGCGTGGTTGC‑CGTGGGCGACCACGGTGTCGTGTTGTTGTCCGACGACCAGGGCCTGCAGTGGCGCCAGGCGAGTTCGGTACCGCTGTCCACACCATTGACGGGCGTGTCTTTCGTGGATGCCAAACGCggg                              >  4:114532/1‑147 (MQ=255)
                                                                                                                              cGGTGCGCGCGTGGTTGC‑CGTGGGCGACCACGGTGTCGTGTTGTTGTCCGACGACCAGGGCCTGCAGTGGCGCCAGGCGAGTTCGGTACCGCTGTCCACACCATTGACGGGCGTGTCTTTCGTGGATGCCAAACGCGGGTGGGCGGTTg                    >  2:74801/1‑149 (MQ=255)
                                                                                                                               ggTGCGCGCGTGGTTGC‑CGTGGGCGACCACGGTGTCGTGTTGTTGTCCGACGACCAGGGCCTGCAGTGGCGCCAGGCGAGTTCGGTACCGCTGTCCACACCATTGACGGGCGTGTCTTTCGTGGATGCCAAACGCGGGTGGGCGGTTgg                   <  2:235946/149‑1 (MQ=255)
                                                                                                                                      gcgTGGTTGC‑CGTGGGCGACCACGGTGTCGTGTTGTTGTCCGACGACCAGGGCCTGCAGTGGCGCCAGGCGAGTTCGGTACCGCTGTCCACACCATTGACGGGCGTGTCTTTCGTGGATGCCAAACGCGGGTGGGCGGTTGGCCACTgg            >  5:114636/1‑149 (MQ=255)
                                                                                                                                       cgTGGTTGC‑CGTGGGCGACCACGGTGTCGTGTTGTTGTCCGACGACCAGGGCCTGCAGTGGCGCCAGGCGAGTTCGGTACCGCTGTCCACACCATTGACGGGCGTGTCTTTCGTGGATGCCAAACGCGGGTGGGCGGTTGGCCACTggg           <  2:158516/149‑1 (MQ=255)
                                                                                                                                       cgTGGTCGC‑CCACGGCAACCACGGTGTCGTGTTGTTGTCCGACGACCAGGGCCTGCAGTGGCGCCAGGCGAGTTCGGTACCGCTGTCCACACCATTGACGGGCGTGTCTTTCGTGGATGCCAAACGCGGGTGGGCGGTTGGCCACTggg           <  1:39821/149‑1 (MQ=255)
                                                                                                                                       cgTGGGTGC‑CGTGGGCGACCACGGTGTCGTGTTGTTGTCCGACGACCAGGGCCTGCAGTGGCGCCAGGAGAGGTCGGTACCGCTGTCCACACCATTGACGGGCGTGTCTTTCGTGGATGCCAAACGCGGGTGGGCGGTTGGCCACTggg           <  7:218158/149‑1 (MQ=255)
                                                                                                                                              gC‑CGTGGGCGACCACGGTGTCGTGTTGTTGTCCGACGACCAGGGCCTGCAGTGGCGCCAGGCGAGTTCGGTACCGCTGTCCACACCATTGACGGGCGTGTCTTTCGTGGATGCCAAACGCGGGTGGGCGGTTGGCCACTGGGGCGCGa     <  7:43594/148‑1 (MQ=255)
                                                                                                                                              gC‑CGTGGGCGACCACGGGGTCGTGTTGTTGTCCGACGACCAGGGCCTGCAGTGGCGCCAGGCGAGTTCGGAACCGCTGTCCACACCATTGACGGGCGTGTCTTTCGTGGATGCCAAACGCGGGTGGGCGGTTGGCCACTGGGGCGCGa     <  5:222867/148‑1 (MQ=255)
                                                                                                                                                 cGTGGGCGACCACGGTGTCGTGTTGTTGTCCGACGACCAGGGCCTGCAGTGGCGCCAGGCGAGTTCGGTACCGCTGTCCACACCATTGACGGGCGTGTCTTTCGTGGATGCCAAACGCGGGTTGGCGGTTGGCCACTGGGGCGCGATcc  <  1:85766/149‑1 (MQ=255)
                                                                                                                                                 cGTGGGCGACCACGGTGTCGTGTTGTTGTCCGACGACCAGGGCCTGCAGTGGCGCCAGGCGAGTTCGGTACCGCTGTCCACACCATTGACGGGCGTGTCTTTCGTGGATGCCAAACGCGGGTGGGCGGTTGGCCACTGGGGCGCGATcc  >  5:174513/1‑149 (MQ=255)
                                                                                                                                                 cGTGGGCGACCACGGTGTCGTGTTGTTGTCCGACGACCAGGGCCTGCAGTGGCGCCAGGCGAGTTCGGTACCGCTGTCCACACCATTGACGGGCGTGTCTTTCGTGGATGCCAAACGCGGGTGGGCGGTTGGCCACTGGGGCGCGATcc  >  5:161306/1‑149 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
ATTCACCGCGGCCTTGCTCGCAGCAGCATTGCCGCTGATGTCTCTTCAGGCCGCGCCGTTGCAGGCCGTGCCGGCCGTACAGGCGAGCAATGCCACGCAGGCCACCATGTTGGCCGCTGGCTGGGCCGGTGCGCGCGTGGTTGC‑CGTGGGCGACCACGGTGTCGTGTTGTTGTCCGACGACCAGGGCCTGCAGTGGCGCCAGGCGAGTTCGGTACCGCTGTCCACACCATTGACGGGCGTGTCTTTCGTGGATGCCAAACGCGGGTGGGCGGTTGGCCACTGGGGCGCGATCC  >  NC_002947/3208794‑3209086

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑