Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,209,677 T→A 7.6% F301I (TTC→ATC) ‡ PP_2813 → BNR domain‑containing protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,209,6770TA 7.6% 190.9 / 4.9 66F301I (TTC→ATC) ‡PP_2813BNR domain‑containing protein
Reads supporting (aligned to +/- strand):  ref base T (29/32);  new base A (2/3);  total (31/35)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.18e-01

AAGCAAGGGCTCTGTCACTTGCGTAGCGGTAGCCGGCTCTCGTGTGCTGGCGGTCGGGCTGGATGGGCTTAGCGTGCAGAGCAGCGATGGCGGGCACAGTTTCCAGGAAACGCAGACCGCGGATGGCCTTTCACTGACGGCGGCGTTGTTCAACGCCGAAGGTGTACCTGTATTGTTTTCACGCCGAGGGGTAATGCCCTACCAGGAGTGAAGGGTTGGCCACTCGCTCAGAAGCCAGGCATCGCAAACCGATGCCTGGCCTGTTTTCTTGCCTGGGCGAATGGGTGGTCGTGGCAC  >  NC_002947/3209529‑3209825
                                                                                                                                                    |                                                                                                                                                    
aaGCAAGGGCTCTGTCACTTGCGTAGCGGTAGCCGGCTCTCGTGTGCTGGCGGTCGGGCTGGATGGGCTTAGCGTGCAGAGCAGCGATGGCGGGCACAGTTTCCAGGAAACGCAGACCGCGGATGGCCTTTCACTGACGGCGGCgttgt                                                                                                                                                      >  4:266078/1‑149 (MQ=255)
   cAAGGGCTCTGTCACTTGCGTAGCGGTAGCCGGCTCTCGTGTGCTGGCGGTCGGGCTGGATGGGCTTAGCGTGCAGAGCAGCGATGGCGGGCACAGTTTCCAGGAAACGCAGACCGCGGATGGCCTTTCACTGACGGCGGCGTTGTTc                                                                                                                                                    >  5:293294/1‑148 (MQ=255)
   cAAGGGCTCTGTCACTTGCGTAGCGGTAGCCGGCTCTCGTGTGCTGGCGGTCGGGCTGGATGGGCTTAGCGTGCAGAGCAGCGATGGCGGGCACAGTTTCCAGGAAACGCAGACCGCGGATGGCCTTTCACTGACGGCGGCGTTGTTc                                                                                                                                                    >  6:3097/1‑148 (MQ=255)
    aaGGGCTCTGTCACTTGCGTAGCGGTAGCCGGCTCTCGTGTGCTGGCGGTCGGGCTGGATGGGCTTAGCGTGCAGAGCAGCGATGGCGGGCACAGTTTCCAGGAAACGCAGACCGCGGATGGCCTTTCACTGACGGCGGCGTTGTTCaa                                                                                                                                                  >  7:281128/1‑149 (MQ=255)
     aGGGCTCTGTCACTTGCGTAGCGGTAGCCGGCTCTCGTGTGCTGGCGGTCGGGCTGGATGGGCTTAGCGTGCAGAGCAGCGATGGCGGGCACAGTTTCCAGGAAACGCAGACCGCGGATGGCCTTTCACTGACGGCGGCGTTGTTCAAc                                                                                                                                                 >  3:226900/1‑149 (MQ=255)
      gggCTCTGTCACTTGCGTAGCGGTAGCCGGCTCTCGTGTGCTGGCGGTCGGGCTGGATGGGCTTAGCGTGCAGAGCAGCGATGGCGGGCACAGTTTCCAGGAAACGCAGACCGCGGATGGCCTTTCACTGACGGCGGCGTTGTTCAACg                                                                                                                                                >  6:43858/1‑149 (MQ=255)
        gCTCTGTCACTTGCGTAGCGGTAGCCGGCTCTCGTGTGCTGGCGGTCGGGCTGGATGGGCTTAGCGTGCAGAGCAGCGATGGCGGGCACAGTTTCCAGGAAACGCAGACCGCGGATGGCCTTTCACTGACGGCGGCGTTGTTCAACGcc                                                                                                                                              <  1:43063/149‑1 (MQ=255)
            tGTCACTTGCGTAGCGGTAGCCGGCTCTCGTGTGCTGGCGGTCGGGCTGGATGGGCTTAGCGTGCAGAGCAGCGATGGCGGGCACAGTTTCCAGGAAACGCAGACCGCGGATGGCCTTTCACTGACGGCGGCGTTGAACAACGCCgc                                                                                                                                            <  5:52756/147‑2 (MQ=255)
              tCACTTGCGTAGCGGTAGCCGGCTCTCGTGTGCTGGCGGTCGGGCTGGATGGGCTTAGCGTGCAGAGCAGCGATGGCGGGCACAGTTTCCAGGAAACGCAGACCGCGGATGGCCTTTCACTGACGGCGGCGTTGTTCAACGCCGaa                                                                                                                                           <  3:266078/146‑1 (MQ=255)
              tCACTTGCGTAGCGGTAGCCGGCTCTCGTGTGCTGGCGGTCGGGCTGGATGGGCTTAGCGTGCAGAGCAGCGATGGCGGGCACAGTTTCCAGGAAACGCAGACCGCGGATGGCCTTTCACTGACGGCGGCGTTGAACAACGCCgcc                                                                                                                                           <  1:329001/146‑3 (MQ=255)
                 cTTGCGTAGCGGTAGCCGGCTCTCGTGTGCTGGCGGTCGGGCTGGATGGGCTTAGCGTGCAGAGCAGCGATGGCGGGCACAGTTTCCAGGAAACGCAGACCGCGGATGGCCTTTCACTGACGGCGGCGTTGTTCAACGCCGAAGGTGTa                                                                                                                                     >  3:300058/1‑149 (MQ=255)
                  ttGCGTAGCGGTAGCCGGCTCTCGTGTGCTGGCGGTCGGGCTGGATGGGCTTAGCGTGCAGAGCAGCGATGGCGGGCACAGTTTCCAGGAAACGCAGACCGCGGATGGCCTTTCACTGACGGCGGCGTTGTTCAACGCCGAAGGTGTAc                                                                                                                                    >  4:36452/1‑149 (MQ=255)
                    gCGTAGCGGTAGCCGGCTCTCGTGTGCTGGCGGTCGGGCTGGATGGGCTTAGCGTGCAGAGCAGCGATGGCGGGCACAGTTTCCAGGAAACGCAGACCGCGGATGGCCTTTCACTGACGGCGGCGTTGTTCAACGCCGAAGGTGTAcc                                                                                                                                   <  6:293294/148‑1 (MQ=255)
                    gCGTAGCGGTAGCCGGCTCTCGTGTGCTGGCGGTCGGGCTGGATGGGCTTAGCGTGCAGAGCAGCGATGGCGGGCACAGTTTCCAGGAAACGCAGACCGCGGATGGCCTTTCACTGACGGCGGCGTTGTTCAACGCCGAAGGTGTAc                                                                                                                                    <  5:254595/147‑1 (MQ=255)
                     cGTAGCGGTAGCCGGCTCTCGTGTGCTGGCGGTCGGGCTGGATGGGCTTAGCGTGCAGAGCAGCGATGGCGGGCACAGTTTCCAGGAAACGCAGACCGCGGATGGCCTTTCACTGACGGCGGCGTTGTTCAACGCCGAAGGTGTACCTg                                                                                                                                 <  3:101489/149‑1 (MQ=255)
                      gTAGCGGTAGCCGGCTCTCGTGTGCTGGCGGTCGGGCTGGATGGGCTTAGCGTGCAGAGCAGCGATGGCGGGCACAGTTTCCAGGAAACGCAGACCGCGGATGGCCTTTCACTGACGGCGGCGTTGTTCAACGCCGAAGGTGTACCTGt                                                                                                                                >  5:31999/1‑149 (MQ=255)
                      gTAGCGGTAGCCGGCTCTCGTGTGCTGGCGGTCGGGCTGGATGGGCTTAGCGTGCAGAGCAGCGATGGCGGGCACAGTTTCCAGGAAACGCAGACCGCGGATGGCCTTTCACTGACGGCGGCGTTGTGCAACGCCGAAGGTGTACCTg                                                                                                                                 >  3:240174/1‑148 (MQ=255)
                         gCGGTAGCCGGCTCTCGTGTGCTGGCGGTCGGGCTGGATGGGCTTAGCGTGCAGAGCAGCGATGGCGGGCACAGTTTCCAGGAAACGCAGACCGCGGATGGCCTTTCACTGACGGCGGCGTTGTTCAACGCCGAAGGTGTACCTGTAtt                                                                                                                             <  3:307479/149‑1 (MQ=255)
                         gCGGTAGCCGGCTCTCGTGTGCTGGCGGTCGGGCTGGATGGGCTTAGCGTGCAGAGCAGCGATGGCGGGCACAGTTTCCAGGAAACGCAGACCGCGGATGGCCTTTCACTGACGGCGGCGTTGTTCAACGCCGAAGGTGTACCTGTAtt                                                                                                                             <  5:286486/149‑1 (MQ=255)
                         gCGGTAGCCGGCTCTCGTGTGCTGGCGGTCGGGCTGGATGGGCTTAGCGTGCAGAGCAGCGATGGCGGGCACAGTTTCCAGGAAACGCAGACCGCGGATGGCCTTTCACTGACGGCGGCGTTGAACAACGCCgc                                                                                                                                            >  6:271970/1‑133 (MQ=255)
                         gCGGTAGCCGGCTCTCGTGTGCTGGCGGTCGGGCTGGATGGGCTTAGCGTGCAGAGCAGCGATGGCGGGCACAGTTTCCAGGAAACGCAGACCGCGGATGGCCTTTCACTGACGGCGGCGTTGAACAACGCCgc                                                                                                                                            <  5:271970/134‑2 (MQ=255)
                          ctgTAGCCGGCTCTCGTGTGCTGGCGGTCGGGCTGGATGGGCTTAGCGTGCAGAGCAGCGATGGCGGGCACAGTTTCCAGGAAACGCAGACCGCGGATGGCCTTTCACTGACGGCGGCGTTGTTCAACGCCGAAGGTGTACCTGTAtt                                                                                                                             <  7:43705/146‑1 (MQ=255)
                          cGGTAGCCGGCTCTCGTGTGCTGGCGGTCGGGCTGGATGGGCTTAGCGTGCAGAGCAGCGATGGCGGGCACAGTTTCCAGGAAACGCAGACCGCGGATGGCCTTTCACTGACGGCGGCGTTGTTCAACGCCGAAGGTGTACCTGTAtt                                                                                                                             <  6:339528/148‑1 (MQ=255)
                               gCCGGCTCTCGTGTGCTGGCGGTCGGGCTGGATGGGCTTAGCGTGCAGAGCAGCGATGGCGGGCACAGTTTCCAGGAAACGCAGACCGCGGATGGCCTTTCACTGACGGCGGCGTTGTTCAACGCCGAAGGTGTACCTGTATTGtttt                                                                                                                        <  5:43858/148‑1 (MQ=255)
                               gCCGGCTCTCGTGTGCTGGCGGTCGGGCTGGATGGGCTTAGCGTGCAGAGCAGCGATGGCGGGCACAGTTTCCAGGAAACGCAGACCGCGGATGGCCTTTCACTGACGGCGGCGTTGTTCAACGCCGAAGGTGTACCTGTATTGTTTTc                                                                                                                       <  4:257075/149‑1 (MQ=255)
                                 cGGCTCTCGTGTGCTGGCGGTCGGGCTGGATGGGCTTAGCGTGCAGAGCAGCGATGGCGGGCACAGTTTCCAGGAAACGCAGACCGCGGATGGCCTTTCACTGACGGCGGCGTTGTTCAACGCCGAAGGTGTACCTGTATTGTTTTCAc                                                                                                                     >  8:266410/1‑149 (MQ=255)
                                  ggCTCTCGTGTGCTGGCGGTCGGGCTGGATGGGCTTAGCGTGCAGAGCAGCGATGGCGGGCACAGTTTCCAGGAAACGCAGACCGCGGATGGCCTTTCACTGACGGCGGCGTTGTTCAACGCCGAAGGTGTACCTGTATTGTTTTCACg                                                                                                                    >  5:80615/1‑149 (MQ=255)
                                         gtgtGCTGGCGGTCGGGCTGGATGGGCTTAGCGTGCAGAGCAGCGATGGCGGGCACAGTTTCCAGGAAACGCAGACCGCGGATGGCCTTTCACTGACGGCGGCGTTGTTCAACGCCGAAGGTGTACCTGTATTGTTTTCACGCCGAg                                                                                                               <  8:281128/147‑1 (MQ=255)
                                         gtgtGCTGGCGGTCGGGCTGGATGGGCTTAGCGTGCAGAGCAGCGATGGCGGGCACAGTTTCCAGGAAACGCAGACCGCGGATGGCCTTTCACTGACGGCGGCGTTGTTCAACGCCGAAGGTGTACCTGTATTGTTTTCACGCCGAg                                                                                                               <  7:266410/147‑1 (MQ=255)
                                         gtgtGCTGGCGGTCGGGCTGGATGGGCTTAGCGTGCAGAGCAGCGATGGCGGGCACAGTTTCCAGGAAACGCAGACCGCGGATGGCCTTTCACTGACGGCGGAGTTGTTCAACGCCGAAGGTGTACCTGTATTGGTTTCACGCCGAggg                                                                                                             >  8:303565/1‑149 (MQ=255)
                                                       gggCTGGATGGGCTTAGCGTGCAGAGCAGCGATGGCGGGCACAGTTTCCAGGAAACGCAGACCGCGGATGGCCTTTCACTGACGGCGGCGTTGTTCAACGCCGAAGGTGTACCTGTATTGtttt                                                                                                                        <  8:105275/124‑1 (MQ=255)
                                                       gggCTGGATGGGCTTAGCGTGCAGAGCAGCGATGGCGGGCACAGTTTCCAGGAAACGCAGACCGCGGATGGCCTTTCACTGACGGCGGCGTTGTTCAACGCCGAAGGTGTACCTGTATTGtttt                                                                                                                        >  7:105275/1‑124 (MQ=255)
                                                       gggCTGGATGGGCTTAGCGTGCAGAGCAGCGATGGCGGGCACAGTTTCCAGGAAACGCAGACCGCGGATGGCCTTTCACTGACGGCGGCGTTGTTCAACGCCGAAGGTGTACCTGTATTGTTTTCACGCCGAGGGGTAATGCCCTAcc                                                                                                <  4:293103/148‑1 (MQ=255)
                                                           tGGATGGGCTTAGCGTGCAGAGCAGCGATGGCGGGCACAGTTTCCAGGAAACGCAGACCGCGGATGGCCTTTCACTGACGGCGGCGTTGTTCAACGCCGAAGGTGTACCTGTATTGTTTTCACGCCGAGCGGTAATGCCCTACCAGGAg                                                                                           >  6:159622/1‑149 (MQ=255)
                                                             gATGGGCGTAGCGTGCAGAGCAGCGATGGCGGGCACAGTTTCCAGGAAACGCAGACCGCGGAGGGCCTTTCACTGACGGCGGCGTTGTTCAACGCCGAAGGTGTACCTGTATTGTTTTCACGCCGAGGGGTAATGCCCTACCAGGAg                                                                                           <  6:67308/147‑1 (MQ=255)
                                                               tGGGCTTAGCGTGCAGAGCAGCGATGGCGGGCACAGTTTCCAGGAAACGCAGACCGCGGATGGCCTTTCACTGACGGCGGCGTTGTTCAACGCCGAAGGTGTACCTGTATTGTTTTCACGCCGAGGGGTAATGCCCTACCAGGAGTg                                                                                         >  8:287225/1‑147 (MQ=255)
                                                                 ggCTTAGCGTGCAGAGCAGCGATGGCGGGCACAGTTTCCAGGAAACGCAGACCGCGGATGGCCTTTCACTGACGGCGGCGTTGTTCAACGCCGAAGGTGTACCTGTATTGTTTTCACGCCGAGGGGTAATGCCCTACCAGGAGTGAAgg                                                                                     <  1:295724/149‑1 (MQ=255)
                                                                  gCTTAGCGTGCAGAGCAGCGATGGCGGGCACAGTTTCCAGGAAACGCAGACCGCGGATGGCCTTTCACTGACGGCGGCGTTGTTCAACGCCGAAGGTGTACCTGTATTGTTTTCACGCCGAGGGGTAATGCCCTACCAGGAGTGAAggg                                                                                    <  4:300058/149‑1 (MQ=255)
                                                                    ttAGCGTGCAGAGCAGCGATGGCGGGCACAGTTTCCAGGAAACGCAGACCGCGGATGGCCTTTCACTGACGGCGGCGTTGTTCAACGCCGAAGGTGTACCTGTATTGTTTTCACGCCGAGGGGTAATGCCCTACCAGGAGTGAAGGGtt                                                                                  >  7:239889/1‑149 (MQ=255)
                                                                      aGCGTGCAGAGCAGCGATGGCGGGCACAGTTTCCAGGAAACGCAGACCGCGGATGGCCTTTCACTGACGGCGGCGTTGTTCAACGCCGAAGGTGTACCTGTATTGTTTTCACGCCGAGGGGTAATGCCCTACCAGGAGTGAAGGGtt                                                                                  <  3:36452/147‑1 (MQ=255)
                                                                          tGCAGAGCAGCGATGGCGGGCACAGTTTCCAGGAAACGCAGACCGCGGATGGCCTTTCACTGACGGCGGCGTTGTTCAACGCCGAAGGTGTACCTGTATTGTTTTCACGCCGAGGGGTAATGCCCTACCAGGAGTGAAGGGTTGGCCAc                                                                            <  6:108220/149‑1 (MQ=255)
                                                                                gcagcGATGGCGGGCACAGTTTCCAGGAAACGCAGACCGCGGATGGCCTTTCACTGACGGCGGCGTTGTTCAACGCCGAAGGTGTACCTGTATTGTTTTCACGCCGAGGGGTAATGCCCTACCAGGAGTGAAGGGTTGGCCACTCGCTc                                                                      <  6:31999/149‑1 (MQ=255)
                                                                                  agcGATGGCGGGCACAGTTTCCAGGAAACGCAGACCGCGGATGGCCTTTCACTGACGGCGGCGTTGTTCAACGCCGAAGGTGTACCTGTATTGTTTTCACGCCGAGGGGTAATGCCCTACCAGGAGTGAAGGGTTGGCCACTCGCTCa                                                                     >  6:46429/1‑148 (MQ=255)
                                                                                     gATGGCGGGCACAGTTTCCAGGAAACGCAGACCGCGGATGGCCTTTCACTGACGGCGGCGTTGTTCAACGCCGAAGGTGTACCTGTATTGTTTTCACGCCGAGGGGTAATGCCCTACCAGGAGTGAAGGGTTGGCCACTCGCTCAg                                                                    >  6:187378/1‑146 (MQ=255)
                                                                                     gATGGCGGGCACAGTTTCCAGGAAACGCAGACCGCGGATGGCCTTTCACTGACGGCGGCGTTGTTCAACGCCGAAGGTGTACCTGTATTGTTTTCACGCCGAGGGGTAATGCCCTACCAGGAGTGAAGGGTTGGCCACTCGCTCAGaa                                                                  <  5:187378/148‑1 (MQ=255)
                                                                                         gCGGGCACAGTTTCCAGGAAACGCAGACCGCGGATGGCCTTTCACTGACGGCGGCGTTGTTCAACGCCGAAGGTGTACCTGTATTGTTTTCACGCCGAGGGGTAATGCCCTACCAGGAGTGAAGGGTTGGCCACTCGCTCAGAAGCCAg                                                             <  4:226900/149‑1 (MQ=255)
                                                                                         gCGGGCACAGTTTCCAGGAAACGCAGACCGCGGATGGCCTTTCACTGACGGCGGCGTTGTTCAACGCCGAAGGTGTACCTGTATTGTTTTCACGCCGAGGGGTAATGCCCTACCAGGAGTGAAGGGTTGGCCACTCGCTCAGAAGCCAg                                                             <  2:333545/149‑1 (MQ=255)
                                                                                             gCACAGTTTCCAGGAAACGCAGACCGCGGATGGCCTTTCACTGACGGCGGCGTTGTTCAACGCCGAAGGTGTACCTGTATTGTTTTCACGCCGAGGGGTAATGCCCTACCa                                                                                               <  5:234478/111‑1 (MQ=255)
                                                                                             gCACAGTTTCCAGGAAACGCAGACCGCGGATGGCCTTTCACTGACGGCGGCGTTGTTCAACGCCGAAGGTGTACCTGTATTGTTTTCACGCCGAGGGGTAATGCCCTACCa                                                                                               >  6:234478/1‑111 (MQ=255)
                                                                                               acaGTTTCCAGGAAACGCAGACCGCGGATGGCCTTTCACTGACGGCGGCGTTGTTCAACGCCGAAGGTGTACCTGTATTGTTTTCACGCCGAGGGGTAATGCCCTACCAGGAGTGAAGGGTTGGCCACTCGCTCAGAAGCCAGGCATCg                                                       >  7:12517/1‑149 (MQ=255)
                                                                                                        aGGAAACGCAGACCGCGGATGGCCTTTCACTGACGGCGGCGTTGTTCAACGCCGAAGGTGTACCTGTATTGTTTTCACGCCGAggg                                                                                                             >  8:22849/1‑86 (MQ=255)
                                                                                                           aaaCGCAGACCGCGGATGGCCTTTCACTGACGGCGGCGTTGTTCAACGCCGAAGGTGTACCTGTATTGTTTTCACGCCGAGGGGTAATGCCCTACCAGGAGTGAAGGGTTGGCCACTCGCTCAGAAGCCAGGCATCGc                                                      <  6:240925/138‑1 (MQ=255)
                                                                                                           aaaCGCAGACCGCGGATGGCCTTTCACTGACGGCGGCGTTGTTCAACGCCGAAGGTGTACCTGTATTGTTTTCACGCCGAGGGGTAATGCCCTACCAGGAGTGAAGGGTTGGCCACTCGCTCAGAAGCCAGGCATCGc                                                      >  5:240925/1‑138 (MQ=255)
                                                                                                               gCAGACCGCGGATGGCCTTTCACTGACGGCGGCGTTGTTCAACGCCGAAGGTGTACCTGTATTGTTTTCACGCCGAGGGGTAATGCCCTACCAGGAGTGAAGGGTTGGCCACTCGCTCAGAAGCCAGGCATCGCAAACCGATgcctggc                                       >  2:95168/1‑149 (MQ=255)
                                                                                                                      gcgGATGGCCTTTCACTGACGGCGGCGTTGTTCAACGCCGAAGGTGTACCTGTATTGTTTTCACGCCGAGGGGTAATGCCCTACCAGGAGTGAAGGGTTGGCCACTCGCTCAGAAGCCAGGCATCGCAAACCGATGCCTGGCCTGtttt                                <  7:287225/149‑1 (MQ=255)
                                                                                                                       cgGATGGCCTTTCACTGACGGCGGCGTTGTTCAACGCCGAAGGTGTACCTGTATTGTTTTCACGCCGAGGGGTAATGCCCTACCATGAGTGAAGGGTTGGCCACTCGCTCAGAAGCCAGGCATCGCAAACCGATGCCTGGCCTGTTTTc                               <  6:80615/149‑1 (MQ=255)
                                                                                                                        ggATGGCCTTTCACTGACGGCGGCGTTGTTCAACGCCGAAGGTGTACCTGTATTGTTTTCACGCCGAGGGGTAATGCCCTACCAGGAGTGAAGGGTTGGCCACTCGCTCAGAAGCCAGGCATCGCAAACCGATGCCTGGCCTGTTTTCt                              >  3:126165/1‑149 (MQ=255)
                                                                                                                         gATGGCCTTTCACTGACGGCGGCGTTGTTCAACGCCGAAGGTGTACCTGTATTGTTTTCACGCCGAGGGGTAATGCCCTACCAGGAGTGAAGGGTTGGCCACTCGCTCAGAAGCCAGGCATCGCAAACCGATGCCTGGCCTGTTTTCtt                             <  5:329767/149‑1 (MQ=255)
                                                                                                                                tttCACTGACGGCGGCGTTGTTCAACGCCGAAGGTGTACCTGTATTGTTTTCACGCCGAGGGGTAATGCCCTACCAGGAGTGAAGGGTTGGCCAc                                                                            >  6:160699/1‑95 (MQ=255)
                                                                                                                                tttCACTGACGGCGGCGTTGTTCAACGCCGAAGGTGTACCTGTATTGTTTTCACGCCGAGGGGTAATGCCCTACCAGGAGTGAAGGGTTGGCCACTCGCTCAGAAGCCAGGCATCGCAAACCGATGCCTGGCCTGTTTTCTTGCCTggg                      >  2:16033/1‑149 (MQ=255)
                                                                                                                                  tCACTGACGGCGGCGTTGTTCAACGCCGAAGGTGTACCTGTATTGTTTTCACGCCGAGGGGTAATGCCCTACCAGGAGTGAAGGGTTGGCCACTCGCTCAGAAGCCAGGCATCGCAAACCGATGCCTGGCCTGTTTTCTTGCCTGGGc                     <  4:41299/148‑1 (MQ=255)
                                                                                                                                   cACTGACGGCGGCGTTGTTCAACGCCGAAGGTGTACCTGTATTGTTTTCACGCCGAGGGGTAATGCCCTACCAGGAGTGAAGGGTTGGCCACTCGCTCAGAAGCCAGGCATCGCAAACCGATgcctg                                         <  5:144554/127‑1 (MQ=255)
                                                                                                                                   cACTGACGGCGGCGTTGTTCAACGCCGAAGGTGTACCTGTATTGTTTTCACGCCGAGGGGTAATGCCCTACCAGGAGTGAAGGGTTGGCCACTCGCTCAGAAGCCAGGCATCGCAAACCGATgcctg                                         >  6:144554/1‑127 (MQ=255)
                                                                                                                                      tGACGGCGGCGTTGTTCAACGCCGAAGGTGTACCTGTATTGTTTTCACGCCGAGGGGTAATGCCCTACCAGGAGTGAAGGGTTGGCCACTCGCTCAGAAGCCAGGCATCGCAAACCGATGCCTGGCCTGTTTTCTTGCCTGGGCGAATg                >  8:105836/1‑149 (MQ=255)
                                                                                                                                        aCGGCGGCGTTGTTCAACGCCGAAGGTGTACCTGTATTGTTTTCACGCCGAGGGGTAATGCCCTACCAGGAGTGAAGGGTTGGCCACTCGCTCAGAAGCCAGGCATCGCAAACCGATGCCTGGCCTGTTTTCTTGCCTGGGCGAATg                <  5:46429/147‑1 (MQ=255)
                                                                                                                                          ttcggcgTTGAACAACGCCGAAGGTGTACCTGTATTGTTTTCACGCCGAGGGGTAATGCCCTACCAGGAGTGAAGGGTTGGCCACTCGCTCAGAAGCCAGGCATCGCAAACCGATGCCTGGCCTGTTTTCTTGCCTGGGCGAATGggtg            >  2:109374/3‑149 (MQ=255)
                                                                                                                                             ggcgTTGTTCAACGCCGAAGGTGTACCTGTATTGTTTTCACGCCGAGGGGTAATGCCCTACCAGGAGTGAAGGGTTGGCCACTCGCTCAGAAGCCAGGCATCGCAAACCGATGCCTGGCCTGTTTTCTTGCCTGGGCGAATGGGTGGTc         >  6:209792/1‑149 (MQ=255)
                                                                                                                                                 ttgttCAACGCCGAAGGTGTACCTGTATTGTTTTCACGCCGAGGGGTAATGCCCTACCAGGAGTGAAGGGTTGGCCACTCGCTCAGAAGCCAGGCATCGCAAACCGATGCCTGGCCTGTTTTCTTGCCTGGGCGAATGGGTGGTCGTgg     <  4:126165/149‑1 (MQ=255)
                                                                                                                                                    ttCAACGCCGAAGGTGTACCTGTATTGTTTTCACGCCGAGGGGTAATGCCCTACCAGGAGTGAAGGGTTGGCCACTCGCTCAGAAGCCAGGCATCGCAAACCGATGCCTGGCCTGTTTTCTTGCCTGGGCGAATGGGTGGTCGTGGCAc  <  5:159622/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
AAGCAAGGGCTCTGTCACTTGCGTAGCGGTAGCCGGCTCTCGTGTGCTGGCGGTCGGGCTGGATGGGCTTAGCGTGCAGAGCAGCGATGGCGGGCACAGTTTCCAGGAAACGCAGACCGCGGATGGCCTTTCACTGACGGCGGCGTTGTTCAACGCCGAAGGTGTACCTGTATTGTTTTCACGCCGAGGGGTAATGCCCTACCAGGAGTGAAGGGTTGGCCACTCGCTCAGAAGCCAGGCATCGCAAACCGATGCCTGGCCTGTTTTCTTGCCTGGGCGAATGGGTGGTCGTGGCAC  >  NC_002947/3209529‑3209825

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑