Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	3,223,223	G→T	11.0%	intergenic (‑530/+30)	PP_2823 ← / ← PP_2824	methyl‑accepting chemotaxis transducer/TetR family transcriptional regulator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	3,223,223	0	G	T	11.0%	152.7 / 8.1	54	intergenic (‑530/+30)	PP_2823/PP_2824	methyl‑accepting chemotaxis transducer/TetR family transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base G (20/28);  new base T (4/2);  total (24/30)
Fisher's exact test for biased strand distribution p-value = 3.89e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.04e-01

GACGCAATGGCGCCAGAGCCTGCAAAAAGCAGGGCAGGCGCTATCGGGGCCTGGTGCTTGGGTTGGATCGGCAAGGCGCGGGGCGGCAGTTGCGCGACGACGGAAGGCGGCGAAGACACGAGCGGTTTGGTGGGGGTGACGCCTTCCAGGGAAGGCGCCGGGTCTACCGGGAGGCGACTTAGGACTTGGCGGTTTTGGGTGGAGAAATCATGACAATGAAGGCGTCAATCAAGGCTTTCCCGCGCTCTGCGCTAACGGGTTCGGACTCGAAAGGCAGCATCATGAGCCAGCCATCAC  >  NC_002947/3223075‑3223371                                                                                                                                                     |                                                                                                                                                     gACGCAATGGCGCCAGAGCCTGCAAAAAGCAGGGCAGGCGCTATCGGGGCCTGGTGCTTGGGTTGGATCGGCAAGGCGCGGGGCGGCAGGTGCGCGACGACGGAAGGCGGCGAAGACACGAGCGGTTTGGTGGGGGTGACGCCTTCCAg                                                                                                                                                      <  3:64158/149‑1 (MQ=255)   cGCAATGGCGCCAGAGCCTGCAAAAAGCAGGGCAGGCGCTATCGGGGCCTGGTGCTTGGGTTGGATCGGCAAGGCGCGGGGCGGCAGTTGCGCGACGACGGAAGGCGGCGAAGACACGAGCGGTTTGGTGGGGGTGACGCCTTCCAggg                                                                                                                                                    <  5:278590/149‑1 (MQ=255)               agagCCTGCAAAAAGCAGGGCAGGCGCTATCGGGGCCTGGTGCTTGGGTTGGATCGGCAAGGCGCGGGGCGGCAGTTGCGCGACGACGGAAGGCGGCGAAGACACGAGCGGTTTGGTGGGGGTGACGCCTTCCAGGGAAGGCGCCGGGt                                                                                                                                        <  5:72056/149‑1 (MQ=255)                   ccTGCAAAAAGCAGGGCAGGCGCTATCGGGGCCTGGTGCTTGGGTTGGATCGGCAAGGCGCGGGGCGGCAGTTGCGCGACGACGGAAGGCGGCGAAGACACGAGCGGTTTGGTGGGGGTGACGCCTTCCAGGGAAGGCGCCGGGTCTAc                                                                                                                                    >  7:108929/1‑149 (MQ=255)                      gCAAAAAGCAGGGCAGGCGCTATCGGGGCCTGGTGCTTGGGTTGGATCGGCAAGGCGCGGGGCGGCAGTTGCGCGACGACGGAAGGCGGCGAAGACACGAGCGGTTTGGTGGGGGTGACGCCTTCCAGGGAAGGCGCCGGGTCTACCgg                                                                                                                                 >  8:265052/1‑149 (MQ=255)                      gCAAAAAGCAGGGCAGGCGCTATCGGGGCCTGGTGCTTGGGTTGGATCGGCAAGGCGCGGGGCGGCAGTTGCGCGACGACGGAAGGCGGCGAAGACACGAGCGGTTTGGTGGGGGTGACGCCTTCCAGGGAAGGCGCCGGGTCTACCgg                                                                                                                                 >  3:48782/1‑149 (MQ=255)                       cAAATAGCAGGGCAGGCGCTATCGGGGCCTGGTGCTTGGGTTGGATCGGTAAGGCGCGGGGCGGCAGTTGCGCGACGACGGAAGGCGGCGAAGACACGAGCGGTTTGGTGGGGGTGACGCCTTCCAGGGAAGGCGCCGGGTCTACCgg                                                                                                                                 <  6:246168/148‑1 (MQ=255)                       cAAAAAGCAGGGCAGGCGCTATCGGGGCCTGGTGCTTGGGTTGGATCGGCAAGGCGCGGGGCGGCAGTTGCGCGACGACGGAAGGCGGCGAAGACACGAGCGGTTTGGTGGGGGTGACGCCTTCCAGGGAAGGCGCCGGGTCTACCgg                                                                                                                                 <  5:335851/148‑1 (MQ=255)                       cAAAAAGCAGGGCAGGCGATATCGGGGCCTGGTGCTTGGGGTGGATCGGCAAGGCGCTGGGCGGCAGTTGCGCGACGACGGAAGGCGGCGAAGACACGAGCGGTTTGGTGGGGGTGACGCCTTCCAGGGAAGGCGCCGGGTCTACCgg                                                                                                                                 <  7:300650/148‑1 (MQ=255)                        aaaaaGCAGGGCAGGCGCTATCGGGGCCTGGTGCTTGGGTTGGATCGGCAAGGCGCGGGGCGGCAGTTGCGCGACGACGGAAGGCGGCGAAGACACGAGCGGGTTGGTGGGGGTGACGCCTTCCAGGGAAGGCGCCGGGTCTACCgg                                                                                                                                 >  7:301281/1‑147 (MQ=255)                           aaGCAGGGCAGGCGCTATCGGGGCCTGGTGCTTGGGTTGGATCGGCAAGGCGCGGGGCGGCAGTTGCGCGACGACGGAAGGCGGCGAAGACACGAGCGGTTTGGTGGGGGTGACGCCTTCCAGGGAAGGCGCCGGGTCTACCGGGAGGc                                                                                                                            >  3:162829/1‑149 (MQ=255)                               agggcaggCGCTATCGGGGCCTGGTGCTTGGGTTGGATCGGCAAGGCGCGGGGCGGCAGTTGCGCGACGACGGAAGGCGGCGAAGACACGAGCGGTTTGGTGGGGGTGACGCCTTCCAGGGAAGGCGCCGGGTCTACCGGGAGGCGACt                                                                                                                        >  1:35445/1‑149 (MQ=255)                                gggcaggCGCTATCGGGGCCTGGTGCTTGGGTTGGATCGGCAAGGCGCGGGGCGGCAGTTGCGCGACGACGGAAGGCGGCGAAGACACGAGCGGTTTGGTGGGGGTGACGCCTTCCCTGGAAGgc                                                                                                                                               >  5:315188/1‑125 (MQ=255)                                gggcaggCGCTATCGGGGCCTGGGGCTTGGGTTGGATCGGCAAGGCGCGGGGCGGCAGTTGCGCGACGACGGAAGGCGGCGAAGACACGAGCGGTTTGGTGGGGGTGACGCCTTCCCTGGAAGgc                                                                                                                                               <  6:315188/125‑1 (MQ=255)                                 ggcaggCGCTATCGGGGCCTGGTGCTTGGGTTGGATCGGCAAGGCGCGGGGCGGCAGTTGCGCGACGACGGAAGGCGGCGAAGACACGAGCGGTTTGGTGGGGGTGACGCCTTCCAGGGAAGGCGCCGGGTCTACCGGGAGGCGACt                                                                                                                        >  4:106730/1‑147 (MQ=255)                                  gcaggCGCTATCGGGGCCGGGTGCTTGGGTTGGATCGGCAAGGCGCGGGGCGGCAGTTGCGCGACGACGGAAGGCGGCGAAGACACGAGCGGTTTGGTGGGGGTGACGCCTTCCAGGGAAGGCGCCGGGTCTACCGGGAGGCGACTTAg                                                                                                                     <  5:87543/149‑1 (MQ=255)                                    aggCGCTATCGGGGCCGGGGGCTTGGGTGTGCGCGGCAAGGCTCGGGGCGGCAGTTGCGCGACGACGGAAGGCGTCGAAGACACGATCGGTTGTGTGGGGGGGCCGCCTTCCAGGGAAGGCGACGGGTCTACCGGGAGGCGACTTAGGa                                                                                                                   <  2:209663/149‑1 (MQ=255)                                      gcgcTATCGGGGCCTGGTGCTTGGGTTGGATCGGCAAGGCGCGGGGCGGCAGTTGCGCGACGACGGAAGGCGGCGAAGACACGAGCGGTTTGGTGGGGGTGACGCCTTCCAGGGAAGGCGCCGGGTCTACCGGGAGGCGACTTAGGACt                                                                                                                 >  6:267714/1‑149 (MQ=255)                                        gcTATCGGGGCGTGGTGCTGGGGGTGGATCGGCAAGGCGCGGGGCGGCAGTTGCGCGACGACGGAAGGCGGCGAAGACACGAGCGGTTTGGTGGGGGTGACTCCTTCCAGGGAAGGCGCCGGGTCTACCGGGAGGCGACTTAGGACTTg                                                                                                               <  4:195290/149‑1 (MQ=255)                                         cTATCGGGGCCTGGTGCTTGGGTTGGATCGGCAAGGCGCGGGGCGGCAGTTGCGCGACGACGGAAGGCGGCGAAGACACGAGCGGTTTGGTGGGGGTGACGCCTTCCAGGGAAGGCGCCGGGTCTACCGGGATGCGACTTAGGACTTgg                                                                                                              <  7:265052/149‑1 (MQ=255)                                               gggCCTGGTGCTTGGGTTGGATCGGCAAGGCGCGGGGCGGCAGTTGCGCGACGACGGAAGGCGGCGAAGACACGAGCGGTTTGGTGGGGGTGACGCCTTCCAGGGAAGGCGCCGGGTCTACCGGGAGGCGACTTAGGACTTGGCGGttt                                                                                                        >  4:323628/1‑149 (MQ=255)                                                 gCCTGGTGCTTGGGTTGGATCGGCAAGGCGCGGGGCGGCAGTTGCGCGACGACGGAAGGCGGCGAAGACACGAGCGGTTTGGTGGGGGTGACGCCTTCCAGGGAAGGCGCCGGGTCTACCGGGAGGCGACTTAGGACTTGGCGGTTTTg                                                                                                      <  2:314367/149‑1 (MQ=255)                                                  ccTGGTGCTTGGGTTGGATCGGCAAGGCGCGGGGCGGCAGTTGCGCGACGACGGAAGGCGGCGAAGACACGAGCGGTTTGGTGGGGGTGACGCCTTCCAGGGAAGGCGCCGGGTCTACCGGGAGGCGACTTAGGACTTGGCGGTTTTgg                                                                                                     <  8:108929/149‑1 (MQ=255)                                                       tGCTTGGGTTGGATCGGCAAGGCGCGGGGCGGCAGTTGCGCGACGACGGAAGGCGGCGAAGACACGAGCGGTTTGGTGGGGGTGACGCCTTCCAGGGAAGGCGCCGGGTCTACCGGGAGGCGACTTAGGACTTGGCGGTTTTGGGTGga                                                                                                <  2:223262/149‑1 (MQ=255)                                                         cTTGGGTTGGATCGGCAAGGCGCGGGGCGGCAGTTGCGCGACGACGGAAGGCGGCGAAGACACGAGCGGTTTGGTGGGGGTGACGCCTTCCAGGGAAGGCGCCGGGTCTACCGGGAGGCGACTTAGGACTTGGCGGTTTTGGGTGgag                                                                                               >  7:299464/1‑148 (MQ=255)                                                          ttGGGTTGGATCGGCAAGGCGCGGGGCGGCAGTTGCGCGACGACGGAAGGCGGCGAAGACACGAGCGGTTTGGTGGGGGTGACGCCTTCCAGGGAAGGCGCCGGGTCTACCGGGAGGCGACTTAGGACTTGGCGGTTTTGGGTGgaga                                                                                              >  6:213416/1‑148 (MQ=255)                                                             ggTTGGATCGGCAAGGCGCGGGGCGGCAGTTGCGCGACGACGGAAGGCGGCGAAGACACGAGCGGTTTGGTGGGGGTGACGCCTTCCAGGGAAGGCGCCGGGTCTACCGGGAGGCGACTTAGGACTTGGCGGTTTTGGGTGGAGAAATc                                                                                          <  3:323628/149‑1 (MQ=255)                                                              gTTGGATCGGCAAGGCGCGGGGCGGCAGTTGCGCGACGACGGAAGGCGGCGAAGACACGAGCGGTTTGGTGGGGGTGACGCCTTCCAGGGAAGGCGCCGGGTCTACCGGGAGGCGACTTAGGACTTGGCGGTTTTGGGTGGAGAAATCa                                                                                         <  4:252774/149‑1 (MQ=255)                                                               ttGGATCGGCAAGGCGCGGGGCGGCAGTTGCGCGACGACGGAAGGCGGCGAAGACACGAGCGGTTTGGTGGGGGTGACGCCTTCCAGGGAAGGCGCCGGGTCTACCGGGAGGCGACTTAGGACTTGGCGGTTTTGGGTGGAGAAATCAt                                                                                        <  8:75933/149‑1 (MQ=255)                                                                    tCGGCAAGGCGCGGGGCGGCAGTTGCGCGACGACGGAAGGCGGCGAAGACACGAGCGGTTTGGTGGGGGTGACGCCTTCCAGGGAAGGCGCCGGGTCTACCGGGAGGCGACTTAGGACTTGGCGGTTTTgg                                                                                                     <  2:256723/131‑1 (MQ=255)                                                                    tCGGCAAGGCGCGGGGCGGCAGTTGCGCGACGACGGAAGGCGGCGAAGACACGAGCGGTTTGGTGGGGGTGACGCCTTCCAGGGAAGGCGCCGGGTCTACCGGGAGGCGACTTAGGACTTGGCGGTTTTgg                                                                                                     >  1:256723/1‑131 (MQ=255)                                                                       gCAAGGCGCGGGGCGGCAGTTGCGCGACGACGGAAGGCGGCGAAGACACGAGCGGTTTGGTGGGGGTGACGCCTTCCAGGGAAGGCGCCGGGTCTACCGGGAGGCGACTTAGGACTTg                                                                                                               >  2:94432/1‑118 (MQ=255)                                                                       gCAAGGCGCGGGGCGGCAGTTGCGCGACGACGGAAGGCGGCGAAGACACGAGCGGTTTGGTGGGGGTGACGCCTTCCAGGGAAGGCGCCGGGTCTACCGGGAGGCGACTTAGGACTTg                                                                                                               <  1:94432/118‑1 (MQ=255)                                                                         aaGGCGCGGGGCGGCAGTTGCGCGACGACGGAAGGCGGCGAAGACACGAGCGGTTTGGTGGGGGTGACGCCTTCCAGGGAAGGCGCCGGGTCTACCGGGAGGCGACTTAGGACTTGGCGGTTTTGGGTGGAGAAATCATGACAATGAAg                                                                              <  4:48782/149‑1 (MQ=255)                                                                           ggCGCGGGGCGGCAGTTGCGCGACGACGGAAGGCGGCGAAGACACGAGCGGTTTGGTGGGGGTGACGCCTTCCAGGGAAGGCGCCGGGTCTACCGGGAGGCGACTTAGGACTTGGCGGTTTTGGGTGGAGAAATCATGACAATGAAGGc                                                                            <  5:267714/149‑1 (MQ=255)                                                                                     ggcAGTTGCGCGACGACGGAAGGCGGCGAAGACACGAGCGGTTTGGTGGGGGTGACGCCTTCCAGGGAAGGCGCCGGGTCTACCGGGAGGCGACTTAGGACTTGGCGGTTTTGGGTGGAGAAATCATGACAATGAAGGCGTCAATCAAg                                                                  <  2:35445/149‑1 (MQ=255)                                                                                                      ggAAGGCGGCGAAGACACGAGCGGTTTGGTGGGGGTGACGCCTTCCAGGGAAGGCGCCGGGTCTACCGGGAGGCGACTTAGGACTTGGCGGTTTTGGGTGGAGAAATCATGACAATGAAGGCGTCAATCAAGGCTTTCCCGCGCTCTgc                                                 >  1:228261/1‑149 (MQ=255)                                                                                                           gcggcgAAGACACGAGCGGTTTGGTGGGGGTGACGCCTTCCAGGGAAGGCGCCGGGTCTACCGGGAGGCGACTTAGGACTTGGCGGTTTTGGGTGGAGAAATCATGACAATGAAGGCGTCAATCAAGGCTTTCCCGCGCTCTGCGCTaa                                            <  8:299464/149‑1 (MQ=255)                                                                                                              gcgAAGACACGTGCGGTTTGGTGGGGGTGACGCCTTCCAGGGAACGCGCCGGGTCTACCGGGATGCGATTTATGACTTGGCTGTTTTGGGTGGAGAAATCAAGACAATGAACGCGTCAATCAAGGCTTTCCCGCGCTCTGCGCTAACgg                                         <  2:259966/149‑1 (MQ=255)                                                                                                                   gACACGAGCGGTTTGGTGGGGGTGACGCCTTCCAGGGAAGGCGCCGGGTCTACCGGGAGGCGACTTAGGACTTGGCGGTTTTGGGTGGAGAAATCATGACAATGAAGGCGTCAATCAAGGCTTTCCCGCGCTCTGCGCTAACGGGTTCg                                    <  8:301281/149‑1 (MQ=255)                                                                                                                     cacGAGCGGTTTGGTGGGGGTGACGCCTTCCAGGGAAGGCGCCGGGTCTACCGGGAGGCGACTTAGGACTTGGCGGTTTTGGGTGGAGAAATCATGACAATGAAGGCGTCAATCAAGGCTTTCCCGCGCTCTGCGCTAACGGGTTCGGa                                  <  2:228261/149‑1 (MQ=255)                                                                                                                           cGGTTTGGTGGGGGTGACGCCTTCCAGGGAAGGCGCCGGGTCTACCGGGAGGCGACTTAGGACTTGGCGGTTTTGGGTGGAGAAATCATGACAATGAAGGCGTCAATCAAGGCTTTCCCGCGCTCTGCGCTAACGGGTTCGGACTCGa                             >  1:27121/1‑148 (MQ=255)                                                                                                                             gTTTGGTGGGGGGGACGCCTTCCAGGGAAGGCGCCGGGTCTACCGGGAGGCGACTTAGGACTTGGCGGTTTTGGGTGGAGAAATCATGACAATGAAGGCGTCAATCAAGGCTTTCCCGCGCTCTGCGCTAACGGGTTCGGACTCGAAAg                          <  6:132352/149‑1 (MQ=255)                                                                                                                              tttGGTGGGGGTGACGCCTTCCAGGGAAGGCGCCGGGTCTACCGGGAGGCGACTTAGGACTTGGCGGTTTTGGGTGGAGAAATCATGACAATGAAGGCGTCAATCAAGGCTTTCCCGCGCTCTGCGCTAACGGGTTCGGACTCGAAAgg                         >  5:331960/1‑149 (MQ=255)                                                                                                                                tggtggGGGTGACGCCTTCCAGGGAAGGCGCCGGGTCTACCGGGAGGCGACTTAGGACTTGGCGGTTTTGGGTGGAGAAATCATGACAATGAAGGCGTCAATCAAGGCTTTCCCGCGCTCTGCGCTAACGGGTTCGGACTCGAAAGgc                        <  4:162829/148‑1 (MQ=255)                                                                                                                                       ggTGACGCCTTCCAGGGAAGGCGCCGGGTCTACCGGGAGGCGACTTAGGACTTGGCGGTTTTGGGTGGAGAAATCATGACAATGAAGGCGTCAATCAAGGCTTTCCCGCGCTCTGCGCTAACGGGTTCGGACTCGAAAGGCAGcatcat                <  5:213416/149‑1 (MQ=255)                                                                                                                                       ggTGACGCCTTCCAGGGAAGGCGCCGGGTCTACCGGGAGGCGACTTAGGACTTGGCGGTTTTGGGTGGAGAAATCATGACAATGAAGGCGTCAATCAAGGCTTTCCCGCGCTCTGCGCTAACGGGTTCGGACTCGAAAGGCAGcatcat                >  6:31164/1‑149 (MQ=255)                                                                                                                                        ccggcGCCTTCCCTGGAAGGCGCCGGGTCTACCGGGAGGCGACTTAGGACTTGGCGGTTTTGGGTGGAGAAATCATGACAATGAAGGCGTCAATCAAGGCTTTCCCGCGCTCTGCGCTAACGGGTTCGGACTCGAAAGGCAGcatcat                >  7:269033/5‑148 (MQ=255)                                                                                                                                        ccggcGCCTTCCCTGGAAGACGCCGGGTCTACCGGGAGGCGACTTAGGACTTGGCGGTTTTGGGTGGAGAAATCATGACAATGAAGGCGTCAATCAAGGCTTTCCCGCGCTCTGCGCTAACGGGTTCGGACTCGAAAGGCAGcatcat                >  2:180478/5‑148 (MQ=255)                                                                                                                                         tGACGCCTTCCAGGGAAGGCGCCGGGTCTACCGGGAGGCGACTTAGGACTTGGCGGTTTTGGGTGGAGAAATCATGACAATGAAGGCGTCAATCAAGGCTTTCCCGCGCTCTGCGCTAACGGGTTCGGACTCGAAAGGCAGCATCATGa              <  5:252486/149‑1 (MQ=255)                                                                                                                                             gCCTTCCCTGGAAGGCGCCGGGTCTACCGGGAGGCGACTTAGGACTTGGCGGTTTTGGGTGga                                                                                                >  2:261756/1‑63 (MQ=255)                                                                                                                                             gCCTTCCCTGGAAGGCGCCGGGTCTACCGGGAGGCGACTTAGGACTTGGCGGTTTTGGGTGga                                                                                                <  1:261756/63‑1 (MQ=255)                                                                                                                                                ttCCAGGGAAGGCGCCGGGTCTACCGGGAGGCGACTTAGGACTTGGCGGTTTTGGGTGGAGAAATCATGACAATGAAGGCGTCAATCAAGGCTTTCCCGCGCTCTGCGCTAACGGGTTCGGACTCGAAAGGCAGCATCATGagccagc        >  5:306690/1‑148 (MQ=255)                                                                                                                                                ttCCAGGGAAGGCGCCGGGTCTACCGGGAGGCGACTTAGGACTTGGCGGTTTTGGGTGGAGAAATCATGACAATGAAGGCGTCAATCAAGGCTTTCCCGCGCTCTGCGCTAACGGGTTCGGACTCGAAAGGCAGCATCATGagccagc        >  3:167252/1‑148 (MQ=255)                                                                                                                                                   cAGGGAAGGCGCCGGGTCTACCGGGAGGCGACTTAGGACTTGGCGGTTTTGGGTGGAGAAATCAt                                                                                        <  2:251552/65‑1 (MQ=255)                                                                                                                                                   cAGGGAAGGCGCCGGGTCTACCGGGAGGCGACTTAGGACTTGGCGGTTTTGGGTGGAGAAATCAt                                                                                        >  1:251552/1‑65 (MQ=255)                                                                                                                                                     gggAAGGCGCCGGGTCTACCGGGAGGCGACTTAGGACTTGGCGGTTTTGGGTGGAGAAATCATGACAATGAAGGCGTCAATCAAGGCTTTCCCGCGCTCTGCGCTAACGGGTTCGGACTCGAAAGGCAGCATCATGAGCCAGCCATCAc  <  5:31164/149‑1 (MQ=255)                                                                                                                                                     |                                                                                                                                                     GACGCAATGGCGCCAGAGCCTGCAAAAAGCAGGGCAGGCGCTATCGGGGCCTGGTGCTTGGGTTGGATCGGCAAGGCGCGGGGCGGCAGTTGCGCGACGACGGAAGGCGGCGAAGACACGAGCGGTTTGGTGGGGGTGACGCCTTCCAGGGAAGGCGCCGGGTCTACCGGGAGGCGACTTAGGACTTGGCGGTTTTGGGTGGAGAAATCATGACAATGAAGGCGTCAATCAAGGCTTTCCCGCGCTCTGCGCTAACGGGTTCGGACTCGAAAGGCAGCATCATGAGCCAGCCATCAC  >  NC_002947/3223075‑3223371

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑