Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	3,262,053	C→T	6.5%	G25R (GGG→AGG)	PP_2859 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	3,262,053	0	C	T	6.5%	179.2 / 2.3	61	G25R (GGG→AGG)	PP_2859	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (31/26);  new base T (2/2);  total (33/28)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.93e-01

CTGCTCCCGCGCACGGTCCTCCCATGCCAGTTGTCCGCGGGCGATGGCGGGAATATCGGCGTAGCAGGTGTCG‑CGGCATTGCTGGTCGATGAAATGACGCACGTATTCGCCCACCACCAGGCCACCAAAATGTGCCTGGAT‑CACCCCGCTAAGCCAGCTTTTGCCACTGGATTCTGGGCCGCACAGTACCAGCACTTTCATTGCTTGCGGCGCACTCATGGCTGTACCACCGCCGGGTCGCGCCGCCATTCCAGCCAGCCGCGTACGGCAATCAGGGTGAGCACGGCGAAGAAG  >  NC_002947/3261907‑3262200                                                                                                                                                     |                                                                                                                                                    cTGCTCCCGCGCACGGTCCTCCCATGCCAGTTGTCCGCGGGCGATGGCGGGAATATCGGCGTAGCAGGTGTCG‑CGGCATTGCTGGTCGATGAAATGACGCACGTATTCGCCCACCACCAGGCCACCAAAATGTGCCTGGAT‑CACCCCGc                                                                                                                                                   >  5:325861/1‑149 (MQ=255)   gCTCCCGCGCACGGTCCTCCCATGCCAGTTGTCCGCGGGCGATGGCGGGAATATCGGCGTAGCAGGTGTCG‑CGGCATTGCTGGTCGATGAAATGACGCACGTATTCGCCCACCACCAGGCCACCAAAATGTGCCTGGAT‑CACCCCGCt                                                                                                                                                  >  5:219354/1‑148 (MQ=255)       ccGCGCACGGTCCTCCCATGCCAGTTGTCCGCGGGCGATGGCGGGAATATCGGCGTAGCAGGTGTCGAGGGCATTGCAGGGGCTTGAAATGACGCCCGTATTAGCCCACCCCCAGGCCCCCAAAATTTGCCGGGATGCACCCCgaaagc                                                                                                                                               >  1:105846/1‑144 (MQ=255)       ccGCGCACGGTCCTCCCATGCCAGTTGTCCGCGGGCGATGGCGGGAATATCGGCGTAGCAGGTGTCG‑CGGCATTGCTGGTCGATGAAATGACGCACGTGTTCGCCCACCACCAGGCCACCAAAATGTGCCTGGAT‑CACCCCACTAAGc                                                                                                                                              <  8:41788/148‑1 (MQ=255)       ccGCGCACGGTCCTCCCATGCCAGTTGTCCGCGGGCGATGGCGGGAATATCGGCGTAGCAGGTGTCG‑CGGCATTGCTGGTCGATGAAATGACGCACGTATTCGCCCACCACCAGGCCACCAAAATGTGCCTGGAT‑CACCCCGCTAAGcc                                                                                                                                             >  3:261360/1‑149 (MQ=255)           gcACGGTCCTCCCATGCCAGTTGTCCGCGGGCGATGGCGGGAATATCGGCCTAGCAGGTGTCG‑CGGCATTGCTGGTCGATGAAATGACGCACGTATTCGCCCACCACCAGGCCACCAAAATGTGCCTGGAT‑CACCCCGCTAAGCCAGCt                                                                                                                                         <  7:177073/149‑1 (MQ=255)              cGGTCCTCCCATGCCAGTTGTCCGCGGGCGATGGCGGGAATATCGGCGTAGCAGGTGTCG‑CGGCATTGCTGGTCGATGAAATGACGCACGTATTCGCCCACCACCAGGCCACCAAAATGTGCCTGGAT‑CACCCCGCTAAGCCAGCtttt                                                                                                                                      <  2:252791/149‑1 (MQ=255)              cGGTCCTCCCATGCCAGTTGTCCGCGGGCGATGGCGGGAATATCGGCGTAGCAGGTGTCG‑CGGCATTGCTGGTCGATGAAATGACGCACGTATTCGCCCACCACCAGGCCACCAAAATGTGCCTGGAT‑CACCCCGCTAAGCCAGCtttt                                                                                                                                      <  6:219354/149‑1 (MQ=255)               ggTCCTCCCATGCCAGTTGTCCGCGGGCGATGGCGGGAATATCGGCGTAGCAGGTGTCG‑CGGCATTGCTGGTCGATGAAATGACGCACGTATTCGCCCACCACCAGGCCACCAAAATGTGCCTGGAT‑CACCCCGCTAAGCCAGCTTTTg                                                                                                                                     >  4:242512/1‑149 (MQ=255)               ggTCCTCCCATGCCAGTTGTCCGCGGGCGATGGCGGGAATATCGGCGTAGCAGGTGTCG‑CGGCATTGCTGGTCGATGAAATGACGCACGTATTCGCCCACCACCAGGCAACCAAAATGTGCCTGGAT‑CACCCCGCTAAGCCAGCTTTTg                                                                                                                                     >  2:29639/1‑149 (MQ=255)                gTCCTCCCATGCCAGTTGTCCGCGGGCGATGGCGGGAATATCGGCGTAGCAGGTGTCG‑CGGCATTGCTGGTCGATGAAATGACGCACGTATTCGCCCACCACCAGGCCACCAAAATGTGCCTGGAT‑CACCCCGCTAAGCCAGCTTTTGc                                                                                                                                    <  6:325861/149‑1 (MQ=255)                    tccCATGCCAGTTGTCCGCGGGCGAAGGCGGGAATATCGGCGTAGCAGGTGTCG‑CGGCATTGCTGGTCGATGAAATGACGCACGTATTCGCCCACCACCAGGCCACCAAAATGTGCCTGGAT‑CACCCCGCTAAGCCAGCTTTTGCCACt                                                                                                                                >  2:117316/1‑149 (MQ=255)                      ccATGCCAGTTGTCCGCGGGCGATGGCGGGAATATCGGCGTAGCAGGTGTCG‑CGGCATTGCTGGTCGATGAAATGACGCACGTATTCGCCCACCACCAGGCCACCAAAATGTGCCTGGAT‑CACCCCGCTAAGCCAGCTTTTGCCACTgg                                                                                                                              <  5:194209/149‑1 (MQ=255)                       cATGCCAGTTGTCCGCGGGCGATGGCGGGAATATCGGCGTAGCAGGTGTCG‑CGGCATTGCTGGTCGATGAAATGACGCACGTATTCGCCCACCACCAGGCCACCAAAATGTGCCTGGAT‑CACCCCGCTAAGCCAGCTTTTGCCACTGGa                                                                                                                             >  1:202352/1‑149 (MQ=255)                        aTGCCAGTTGTCCGCGGGCGATGGCGGGAATATCGGCGTAGCAGGTGTCG‑CGGCATTGCTGGTCGATGAAATGACGCACGTATTCGCCCACCACCAGGCCACCAAAATGTGCCTGGAT‑CACCCCGCTAAGCCAGCTTTTGCCACTGGAt                                                                                                                            >  6:256813/1‑149 (MQ=255)                            cAGTTGTCCGCGGGCGATGGCGGGAATATCGGCGTAGCAGGTGTCG‑CGGCATTGCTGGTCGATGAAATGACGCACGTATTCGCCCACCACCAGGCCACCAAAATGTGCCTGGAT‑CACCCTGCTAAGCCAGCTTTTGCCACTGGATTCTg                                                                                                                        >  6:89957/1‑149 (MQ=255)                            cAGTTGTCCGCGGGCGATGGCGGGAATATCGGCGTAGCAGGTGTCG‑CGGCATTGCTGGTCGATGAAATGACGCACGTATTCGCCCACCACCAGGCCACCAAAATGTGCCTGGAT‑CACCCTGCTAAGCCAGCTTTTGCCACTGGATTCTg                                                                                                                        >  6:94735/1‑149 (MQ=255)                            cAGTTGTCCGCGGGCGATGGCGGGAATATCGGCGTAGCAGGTGTCG‑CGGCATTGCTGGTCGATGAAATGACGCACGTATTCGCCCACCACCAGGCCACCAAAATGTGCCTGGAT‑CACCCCGCTAAGCCAGCTTTTGCCACTGGATTCTg                                                                                                                        <  2:202352/149‑1 (MQ=255)                             aGTTGTCCGCGGGCGATGGCGGGAATATCGGCGTAGCAGGTGTCG‑CGGCATTGCTGGTCGATGAAATGACGCACGTATTCGCCCACCACCAGGCCACCAAAATGTGCCTGGAT‑CACCCCGCTAAGCCAGCTTTTGCCACTGGATTCTgg                                                                                                                       >  4:42220/1‑149 (MQ=255)                                   ccGCGGGCGATGGCGGGAATATCGGCGTAGCAGGTGTCG‑CGGCATTGCTGGGCGATGAAATGACGCACGTATTCGCCCACCACCAGGCCACCAAAATGTGCCTGGAT‑CACCCCGCTAAGCCAGCTTTTGCCACTGGATTCTGGGCCGca                                                                                                                 <  6:216369/149‑1 (MQ=255)                                           gATGGCGGGAATATCGGCGTAGCAGGTGTCG‑CGGCATTGCTGGTCGATGAAATGACGCACGTATTCGCCCACCACCAGGCCACCAAAATGTGCCTGGAT‑CACCCCGCTAAGCCAGCTTTTGCCACTGGATTCTGGGCCGCACAGTAc                                                                                                           >  8:145372/1‑147 (MQ=255)                                             tGGCGGGAATATCGGCGTAGCAGGTGTCG‑CGGCATTGCTGGTCGATGAAATGACGCACGTATTCGCCCACCACCAGGCCACCAAAATGTGCCTGGAT‑CACCCCGCTAAGCCAGCTTTTGCCACTGGATTCTGGGCCGCACAGTACCAGc                                                                                                       <  3:42220/149‑1 (MQ=255)                                               gCGGGAATATCGGCGTAGCAGGTGTCG‑CGGCATTGCTGGTCGATGAAATGACGCACGTATTCGCCCACCACCAGGCCACCAAAATGTGCCTGGAT‑CACCCCGCTAAGCCAGCTTTTGc                                                                                                                                    <  6:253329/118‑1 (MQ=255)                                                      tatCGGCGTAGCAGGTGTCG‑CGGCATTGCTGGTCGATGAAATGACGCACGTATTCGCCCACCACCAGGCCACCAAAATGTGCCTGGAT‑CACCCCGCTAAGCCAGCTTTTGCCACTGGATTCTGGGCCGCACAGTACCAGCACTTTCAtt                                                                                              <  1:124296/149‑1 (MQ=255)                                                             gTAGCAGGTGTCG‑CGGCATTGCTGGTCGATGAAATGACGCACGTATTCGCCCACCACCAGGCCACCAAAATGTGCCTGGAT‑CACCCCGCTAAGCCAGCTTTTGCCACTGGATTCTGGGCCGCACAGTACCAGCACTTTCATTGCTTgcg                                                                                       <  7:195447/149‑1 (MQ=255)                                                                 cAGGTGTCG‑CGGCATTGCTGGTCGATGAAATGACGCACGTATTCGCCCACCACCAGGCCACCAAAATGTGCCTGGAT‑CACCCCGCTAAGCCAGCTTTTGCCACTGGATTCTGGGCCGCACAGTACCAGCACTTTCATTGCTTgcggcg                                                                                    >  6:79584/1‑148 (MQ=255)                                                                   ggTGTCG‑CGGCATTGCTGGTCGATGAAATGACGCACGTATTCGCCCACCACCAGGCCACCAAAATGTGCCTGGAT‑CACCCCGCTAAGCCAGCTTTTGCCACTGGATTCTGGGCCACACAGTACCAGCACTTTCATTGCTTGCGGCGCAc                                                                                 >  2:152275/1‑149 (MQ=255)                                                                    gtgtCG‑CGGCATTGCTGGTCGATGAAATGACGCACGTATTCGCCCACCACCAGGCCACCAAAATGTGCCTGGAT‑CACGCCGCTAAGCCAGCTTTTGCCACTGGa                                                                                                                             >  1:107487/1‑104 (MQ=255)                                                                    gtgtCG‑CGGCATTGCTGGTCGATGAAATGACGCACGTATTCGCCCACCACCAGGCCACCAAAATGTGCCTGGAT‑CACCCCGCTAAGCCAGCTTTTGCCACTGGa                                                                                                                             <  2:107487/104‑1 (MQ=255)                                                                               aTTGCTGGTCGATGAAATGACGCACGTATTCGCCCACCACCAGGCCACCAAAATGTGCCTGGAT‑CACCCCGCTAAGCCAGCTTTTGCCACTGGATTCTGGGCCGCACAGTACCAGCACTTTCATTGCTTGCGGCGCACTCATGGCTGTa                                                                      <  1:117316/149‑1 (MQ=255)                                                                                   cTGGTCGATGAAATGACGCACGTATTCGCCCACCACCAGGCCACCAAAATGTGCCTGGAT‑CACCCCGCTAAGCCAGCTTTTGCCACTGGATTCTGGGCCGCACAGTACCAGCACTTTCATTGCTTGCGGCGCACTCATGGCTGTaccac                                                                  >  6:35174/1‑149 (MQ=255)                                                                                    tGGTCGATGAAATGACGCACGTATTCGCCCACCACCAGGCCACCAAAATGTGCCTGGAT‑CACCCCGCTAAGCCAGCTTTTGCCACTGGATTCTGGGCCGCACAGTACCAGCACTTTCATTGCTTGCGGCGCACTCATGGCTGTaccacc                                                                 >  4:15440/1‑149 (MQ=255)                                                                                     ggTCGATGAAATGACGCACGTATTCGCCCACCACCAGGCCACCAAAATGTGCCTGGAT‑CACCCCGCTAAGCCAGCTTTTGCCACTGGATTCTGGGCCGCACAGTACCAGCACTTTCATTGCTTGCGGCGCACTCATGGCTGTACCAccg                                                                >  3:75049/1‑149 (MQ=255)                                                                                      gTCGATGAAATGACGCACGTATTCGCCCACCACCAGGCCACCAAAATGTGCCTGGTT‑CACCCCGCTAAGCCAGCTTTTGCCACTGGATTCTGGGCCGCACAGTACCAGCACTTTCATTGCTTGCGGCGCACTCATGGCTGTACCAccgc                                                               >  3:161786/1‑149 (MQ=255)                                                                                       tCGATGAAATGACGCACGTATTCGCCCACCACCAGGCCACCAAAATGTGCCTGGAT‑CACCCCGCTAAGCCAGCTTTTGCCACTGGATTCTGGGCCGCACAGTACCAGCACTTTCATTGCTTGCGGCGCACTCATGGCTGTACCAccgcc                                                              >  6:159382/1‑149 (MQ=255)                                                                                         gATGAAATGACGCACGTATTCGCCCACCACCAGGCCCCCAAAATGTGCCTGGAT‑CACCCCGCTAAGCCAGCTTTTGCCACTGGATTCTGGGCCGCACAGTACCAGCAGTGTCATTGCTTGCGGCGGACTCATGGCTGTACCACCGCCgg                                                            >  4:322056/1‑149 (MQ=255)                                                                                          aTGAAATGACGCACGTATTCGCCCACCACCAGGCCACCAAAATGTGCCTGGAT‑CACCCCGCTAAGCCAGCTTTTGCCACTGGATTCTGGGCCGCACAGTACCAGCACTTTCATTGCTTGCGGCGCACTCATGGCTGTACCACCGCCggg                                                           <  3:275268/149‑1 (MQ=255)                                                                                          aTGAAATGACGCACGTATTCGCCCACCACCAGGCCACCAAAATGTGCCTGGAT‑CACCCCGCTAAGCCAGCTTTTGCCACTGGATTCTGGGCCGCACAGTACCAGCACTTTCATTGCTTGCGGCGCACTCATGGCTGTACCACCGCCggg                                                           <  3:242512/149‑1 (MQ=255)                                                                                              aaTGACGCACGTATTCGCCCACCACCAGGCCACCAAAATGTGCCTGGAT‑CACCCCGCTAAGCCAGCTTTTGCCACTGGATTCTGGGCCGCACAGTACCAGCACTTTCATTGCTTGCGGCGCACTCATGGCTGTACCACCGCCGGGTcgc                                                       <  5:159382/149‑1 (MQ=255)                                                                                                      aCGTATTCGCCCACCACCAGGCCACCAAAATGTGCCTGGAT‑CACCCCGCTAAGCCAGCTTTTGCCACTGGATTCTGGGCCGCACAGTACCAGCACTTTCATTGCTTGCGGCGCACTCATGGCTGTACCACCGCCGGGTCGCGCCGCCAt                                               >  2:191902/1‑149 (MQ=255)                                                                                                        gTATTCGCCCACCACCAGGCCACCAAAATGTGCCTGGAT‑CACCCCGCTAAGCCAGCTTTTGCCACTGGATTCTGGGCCGCACAGTACCAGCACTTTCATTGCTTGCGGCGCACTCATGGCTGTACCACCGCCGGGTCGCGCCGCCATTc                                             >  1:248283/1‑149 (MQ=255)                                                                                                           ttCGCCCACCACCAGGCCACCAAAATGTGCCTGGAT‑CACCCCGCTAAGCCAGCTTTTGCCACTGGATTCTGGGCCGCACAGTACCAGCACTTTCATTGCTTGCGGCGCACTCATGGCTGTACCACCGCCGGGTCGCGCCGCCATTccag                                          >  1:269204/1‑149 (MQ=255)                                                                                                           ttCGCCCACCACCAGGCCACCAAAATGTGCCTGGAT‑CACCCCGCTAAGCCAGCTTTTGCCACTGGATTCTGGGCCACACAGTACCAGCACTTTCATTGCTTGCGGCGCACTCATGGCTGTACCACCGCCGGGTCGCGCCGCCATTcca                                           <  1:152275/148‑1 (MQ=255)                                                                                                             cGCCCACCACCAGGCCACCAAAATGTGCCTGGAT‑CACCCCGCTAAGCCAGCTTTTGCCACTGGATTCTGGGCCGCACAGTACCAGCACTTTCATTGCTTGCGGCGCACTCATGGCTGTACCACCGCCGGGTCGCGCCGCCATTccagc                                         >  7:313980/1‑148 (MQ=255)                                                                                                                ccaccaccaGGCCAACAAACTGTGCCTGGAT‑AACCCCGATAAGCAAGCTTTTGCCACTGGATTCAGAGCCGCACAGTACCAGCACTTACATTGCTTGCGGAGCACTCATAGCAGTACCACCGCCGAGTCGCGCCTCCATTGcagccagc                                     >  5:123946/1‑149 (MQ=255)                                                                                                                   ccaccaGGCCACCAAAATGTGCCTGGAT‑CACCCCGCTAAGCCAGCTTTTGCCACTGGATTCTGGGCCGCACAGTACCAGCACTTTCATTGCTTGCGGCGCACTCATGGCTGTACCACCGCCGGGTCGCGCCGCCATTCCAGCCAGCcgc                                  <  4:261360/149‑1 (MQ=255)                                                                                                                   ccaccaGGCCACCAAAATGTGCCTGGAT‑CACCCCGCTAAGCCAGCTTTTGCCACTGGATTCTGGGCCGCACAGTACCAGCACTTTCATTGCTTGCGGCGCACTCATGGCTGTACCACCGCCGGGTCGCGCCGCCATTCCAGCCAGCcgc                                  <  2:269204/149‑1 (MQ=255)                                                                                                                    caccaGGCCACCAAAATGTGCCTGGAT‑CACCCCGCTAAGCCAGCTTTTGCCACTGGATTCTGGGCCGCACAGTACCAGCACTTTCATTGCTTGCGGCGCACTCATGGCTGTACCACCGCCGGGTCGCGCCGCCATTCCAGCCAGCcgcg                                 >  2:213822/1‑149 (MQ=255)                                                                                                                     accaGGCCACCAAAATGTGCCTGGAT‑CACCCCGCTAAGCCAGCTTTTGCCACTGGATTCTGGGCCGCACAGTACCAGCACTTTCATTGCTTGCGGCGCACTCATGGCTGTACCACCGCCGGGTCGCGCCGCCATTCCAGCCAGCcgcg                                 >  2:994/1‑148 (MQ=255)                                                                                                                      ccaGGCCACCAAAATGTGCCTGGAT‑CACCCCGCTAAGCCAGCTTTTGCCACTGGATTCTGGGCCGCACAGTACCAGCACTTTCATTGCTTGCGGCGCACTCATGGCTGTACCACCGCCGGGTCGCGCCGCCATTCCAGCCAGCCGCGTa                               <  5:256813/149‑1 (MQ=255)                                                                                                                        aGGCCACCAAAATGTGCCTGGAT‑CACCCCGCTAAGCCAGCTTTTGCCACTGGATTCTGGGCCGCACAGTACCAGCACTTTCATTGCTTGCGGCGCACTCATGGCTGTACCACCGCCGGGTCGCGCCGCCATTCCAGCCAGCCGCGTACg                             >  6:10722/1‑149 (MQ=255)                                                                                                                            caccaAAATGTGCCTGGAT‑CACCCCGCTAAGCCAGCTTTTGCCACTGGATTCTGGGCCGCACAGTACCAGCACTTTCATTGCTTGCGGCGCACTCATGGCTGTACCACCGCCGGGTCGCGCCGCCATTCCAGCCAGCCGCGTACGGCaa                         >  4:160455/1‑149 (MQ=255)                                                                                                                                 aaaTGTGCCTGGAT‑CACCCCGCTAAGCCAGCTTTTGCCACTGGATTCTGGGCCGCACAGTACCAGCACTTTCATTGCTTGCGGCGCACTCATGGCTGTACCACCGCCGGGTCGCGCCGCCATTCCAGCCAGCCGCGTACGGCAATCAgg                    >  7:208235/1‑149 (MQ=255)                                                                                                                                   aTGTGCCTGGAT‑CACCCTGCTAAGCCAGCTTTTGCCACTGGATTCTGGGCCGCACAGTACCAGCACTTTCATTGCTTGCGGCGCACTCATGGCTGTACCACCGCCGGGTCGCGCCGCCATTCCAGCCAGCCGCGTACGGCAATCAGGGt                  <  5:94735/149‑1 (MQ=255)                                                                                                                                   aTGTGCCTGGAT‑CACCCTGCTAAGCCAGCTTTTGCCACTGGATTCTGGGCCGCACAGTACCAGCACTTTCATTGCTTGCGGCGCACTCATGGCTGTACCACCGCCGGGTCGCGCCGCCATTCCAGCCAGCCGCGTACGGCAATCAGGGt                  <  5:89957/149‑1 (MQ=255)                                                                                                                                    tgtgCCTGGAT‑CACCCCGCTAAGCCAGCTTTTGCCACTGGATTCTGGGCCGCACAGTACCAGCACTTTCATTGCTTGCGGCGCACTCATGGCTGTACCACCGCCGGGTCGCGCCGCCATTCCAGCCAGCCGCGTACGGCAATCAGGGTg                 <  2:105846/149‑1 (MQ=255)                                                                                                                                        ccTGGAT‑CACCCCGCTAAGCCAGCTTTTGCCACTGGATTCTGGGCCGCACAGTACCAGCACTTTCATTGCTTGCGGCGCACTCATGGCTGTACCACCGCCGGGTCGCGCCGCCATTCCAGCCAGCCGCATACGGCAATCAGGGTGAGc              <  8:313980/148‑1 (MQ=255)                                                                                                                                         cTGGAT‑CACCCCGCTAAGCCAGCTTTTGCCACTGGATTCTGGGCCTCACAGTACCAGCACTTTCATTGCTTGCGGCGCACTCATGGCTGTACCACCGCCGGGTCGCGCCGCCATTCCAGCCAGCCGCGTACGGCAATCAGGGTGAGCa             <  1:213822/148‑1 (MQ=255)                                                                                                                                            gAT‑CACCCCGCTAAGCCAGCTTTTGCCACTGGATTCTGGGCCGCACAGTACCAGCACTTTCATTGCTTGCGGCGCACTCATGGCTGTACCACCGCCGGGTCGCGCCGCCATTCCAGCCAGCCGCGTACGGCAATCAGGGTGAGCACGGc         >  2:107287/1‑149 (MQ=255)                                                                                                                                            gAT‑CACCCCGCTAAGCCAGCTTTTGCCACTGGATTCTGGGCCGCACAGTACCAGCACTTTCATTGCTTGCGGCGCACTCATGGCTGTACCACCGCCGGGTCGCGCCGCCATTCCAGCCAGCCGCGTACGGCAATCAGGGTGAGCACGGc         <  8:208235/149‑1 (MQ=255)                                                                                                                                              t‑CACCCCGCTAAGCCAGCTTTTGCCACTGGATTCTGGGCCGCACAGTACCAGCACTTTCATTGCTTGCGGCGCACTCATGGCTGTACCACCGCCGGGTCGCGCCGCCATTCCAGCCAGCCGCGTACGGCAATCAGGGTGAGCACGGCga       <  4:75049/149‑1 (MQ=255)                                                                                                                                                   cccGCTAAGCCAGCTTTTGCCACTGGATTCTGGGCCGCACAGTACCAGCACTTTCATTGCTTGCGGCGCACTCATGGCTGTACCACCGCCGGGTCGCGCCGCCATTCCAGCCAGCCGCGTACGGCAATCAGGGTGAGCACGGCgaagaa   <  5:79584/149‑1 (MQ=255)                                                                                                                                                   cccGCTAAGCCAGCTTTTGCCACTGGATTCTGGGCCGCACAGTACCAGCACTTTCATTGCTTGCGGCGCACTCATGGCTGTACCACCGCCGGGTCGCGCCGCCATTCCAGCCAGCCGCGTACGGCAATCAGGGTGAGCACGGCgaagaa   <  1:994/149‑1 (MQ=255)                                                                                                                                                    ccGCTAAGCCAGCTTTTGCCACTGGATTCTGGGCCGCACAGTACCAGCACTTTCATTGCTTGCGGCGCACAAATGGCTGTACCACCGCCGGGTCGCGCCGCCATTCCAGCCAGCCGCGTAAGGCAATCAGGGTGAGCACGGCGaagaag  >  1:274782/1‑149 (MQ=255)                                                                                                                                                     |                                                                                                                                                    CTGCTCCCGCGCACGGTCCTCCCATGCCAGTTGTCCGCGGGCGATGGCGGGAATATCGGCGTAGCAGGTGTCG‑CGGCATTGCTGGTCGATGAAATGACGCACGTATTCGCCCACCACCAGGCCACCAAAATGTGCCTGGAT‑CACCCCGCTAAGCCAGCTTTTGCCACTGGATTCTGGGCCGCACAGTACCAGCACTTTCATTGCTTGCGGCGCACTCATGGCTGTACCACCGCCGGGTCGCGCCGCCATTCCAGCCAGCCGCGTACGGCAATCAGGGTGAGCACGGCGAAGAAG  >  NC_002947/3261907‑3262200

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑