Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	3,349,962	C→G	7.2%	T153T (ACC→ACG) ‡	PP_2945 →	two‑component system sensor histidine kinase/response regulator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	3,349,962	0	C	G	7.2%	169.5 / 4.5	56	T153T (ACC→ACG) ‡	PP_2945	two‑component system sensor histidine kinase/response regulator
Reads supporting (aligned to +/- strand):  ref base C (28/23);  new base G (2/2);  total (31/25)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.52e-01

AGGACCCGCTGTTGCAGGTAGTCGTCTGCACTGCCTACTCGGACTATTCCTGGGATGAGTTGCTCGACCGGCTGCAGGCCCACGATCGCTTGCT‑GATCCTGAAAAAACCGTTCGACAACATCGAGGTCCAGCAAATGGCCAGCACCTTGCTGACCAAGTGGGAAATGACCCAACGAGCCTCGCTGAAAATGCACCAGCTCGAACAGCGGGTAGAGCGGCGTACCCAGCAACTGACCCAGGCCAGCGAGGCGCTGCAGCAGGAGATCGAGGAGCGCAAGCAGCTGGAAA  >  NC_002947/3349817‑3350104                                                                                                                                                   |                                                                                                                                               agGACCCGCTGTTGCAGGTAGTCGTCTGCACTGCCTACTCGGACTATTCCTGGGATGAGTTGCTCGACCAGCTGCAGGCCCACGATCGCTTGCT‑GATCCTGAAAAAACCGTTCGACAACATCGAGGTCCAGCAAATGGCCAGCACCTTg                                                                                                                                             <  8:78944/149‑1 (MQ=255)   gACCCGCTGTTGCAGGTAGTCGTCTGCACTGCCTACTCGGACTATTCCTGGGATGAGTTGCTCGACCGGCTGCAGGCCCACGATCGCTTGCT‑GATCCTGAAAAAACCGTTCGACAACATCGAGGTCCAGCAAATGGCCAGCACCTTGCt                                                                                                                                           >  7:117527/1‑149 (MQ=255)        gCTGTTGCAGGTAGTCGTCTGCACTGCCTACTCGGACTATTCCTGGGATGAGTTGCTCGACCGGCCGCAGGCCCACGATCGCTTGCT‑GATCCTGAAAAAACCGTTCGACAACATCGAGGTCCAGCAAATGGTCAGCAAGGTGCTGGCCa                                                                                                                                      >  3:315878/1‑149 (MQ=255)              gCAGGTAGTCGTCTGCACTGCCTACTCGGACTATTCCTGGGATGAGTTGCTCGACCGGCCGCAGGCCCACGATCGCTTGCT‑GATCCTGAAAAAACCGTTCGACAACATCGAGGTCCAGCAAATGGTCAGCAAGGTGCTGGCCatttgc                                                                                                                                 <  4:315878/148‑6 (MQ=255)              gCAGGTAGTCGTCTGCACTGCCTACTCGGACTATTCCTGGGATGAGTTGCGCGACCGGCTGCAGGCCCACGATCGCTTGCT‑GATCCTGAAAAAACCGTTCGACAACATCGAGGTCCAGCAAATGGCCAGCACCTTGCTGACCAAGTgg                                                                                                                                 <  8:191631/148‑1 (MQ=255)                aGGTAGTCGTCTGCACTGCCTACTCGGACTATTCCTGGGATGAGTTGCTCGACCGGCTGCAGGCCCACGATCGCTTGCT‑GATCCTGAAAAAACCGTTCGACAACATCGAGGTCCAGCAAATGGCCAGCACCTTGCTGACCAAGTGGGa                                                                                                                               >  3:9795/1‑148 (MQ=255)                        gtcTGCACTGCCTACTCGGACTATTCCTGGGATGAGATGCTCGACCGGCTGCAGGACCACGATCCCTTGCT‑GATAATGAAAAAACCGTTCGACAAAATCGAGGTCCAGCAAATGGCAAGCACCTTGCTGACAAAGTGTGAAATGAcac                                                                                                                       >  7:189918/1‑146 (MQ=255)                           tGCACTGCCTACTCGGACTATTCCTGGGATGAGTTGCTCGACCGGCTGCAGGCCCACGATCGCTTGCT‑GATCCTGAAAAAACCGTTCGACAACATCGAGGTCCAGCAAATGGACAGCAAGGTGCTGGCCatttgct                                                                                                                                >  3:54706/1‑130 (MQ=255)                           tGCACTGCCTACTCGGACTATTCCTGGGATGAGTTGCTCGACCGGCTGCAGGCCCACGATCGCTTGCT‑GATCCTGAAAAAACCGTTCGACAACATCGAGGTCCAGCAAATGGACAGCAAGGTGCTGGCCatttgct                                                                                                                                <  4:54706/136‑7 (MQ=255)                               cTGCCTACTCGGACTATTCCTGGGATGAGTTGCTCGACCGGCTGCAGGCCCACGATCGCTTGCT‑GATCCTGAAAAAACCGTTCGACAACATCGAGGTCCAGCAAATGGCCAGCACCTTGCTGACCAAGTGGGAAATGACCCAACGAGc                                                                                                                >  4:17473/1‑148 (MQ=255)                               cTGCCTACTCGGACTATTCCTGGGATGAGTTGCTCGAACGGCTGCAGGCCCACGATCGCTTGCT‑GATCCTGAAAAAACCGTTCGACAACATCGAGGTCCAGCAAATGGCCAGCACCTTGCTGACCAAGTGGGAAATGACCCAACGAGc                                                                                                                >  4:21999/1‑148 (MQ=255)                                tGCCTACTCGGACTATTCCTGGGATGAGTTGCTCGACCGGCTGCAGGCCCACGATCGCTTGCT‑GATCCTGAAAAAACCGTTCGACAACATCGAGGTCCAGCAAATGGCCAGCAcc                                                                                                                                                <  6:225743/115‑1 (MQ=255)                                  ccTACTCGGACTATTCCTGGGATGAGTTGCTCGACCGGCTGCAGGCCCACGATCGCTTGCT‑GATCCTGAAAAAACCGTTCGACAACATCGAGGTCCAGCAAATGGCCAGCACCTTGCTGACCAAGTGGGAAATGACCCAACGAGCCTc                                                                                                             <  1:284227/148‑1 (MQ=255)                                    tACTCGGACTATTCCTGGGATGAGTTGCTCGACCGGCTGCAGGCCCACGATCGCTTGCT‑GATCCTGAAAAAACCGTTCGACAACATCGAGGTCCAGCAAATGGCCAGCACCTTGCTGACCAAGt                                                                                                                                   >  8:265009/1‑124 (MQ=255)                                    tACTCGGACTATTCCTGGGATGAGTTGCTCGACCGGCTGCAGGCCCACGATCGCTTGCT‑GATCCTGAAAAAACCGTTCGACAACATCGAGGTCCAGCAAATGGCCAGCACCTTGCTGACCAAGt                                                                                                                                   <  7:265009/124‑1 (MQ=255)                                     aCTCGGACTATTCCTGGGATGAGTTGCTCGACCGGCTGCAGGCCCACGATCGCTTGCT‑GATCCTGAAAAAACCGTTCGACAACATCGAGGTCCAGCAAATGGCCAGCACCTTGCTGACCAAGTGGGAAATGACCCAACGAGCCTCGCt                                                                                                          >  8:285170/1‑148 (MQ=255)                                       tCGGACTATTCCTGGGATGAGTTGCTCGACCGGCTGCAGGCCCACGATCGCTTGCT‑GATCCTGAAAAAACCGTTCGACAACATCGAGGTCCAGCAAATGGCCAGCACCTTGCTGACCAAGTGGGAAATGACCCAACGAGCCTCGCTGa                                                                                                        >  2:167581/1‑148 (MQ=255)                                         ggACTATTCCTGGGATGAGTTGCTCGACCGGCTGCCGGCCCACGATCGCTTGCT‑GATCCTGAAAAAACCGTTCGCCAACATCGAGGTCCAGCAAATGGCCAGCACCGTGCTGACCAAGTGGGAAATGACCCAACGAGACTCGCTGaaaa                                                                                                     >  6:243079/1‑149 (MQ=255)                                         ggACTATTCCTGGGATGAGTTGCTCGACCGGCTGCAGGCCCACGATCGCTTGCT‑GATCCTGAAAAAACCGTTCGACAACATCGAGGTCCAGCAAATGGCCAGCACCTTGCTGACCAAGTGGGAAATGACCCAACGAGCCTCGCTGaaaa                                                                                                     >  6:143382/1‑149 (MQ=255)                                          gACTATTCCTGGGATGAGTTGCTCGACCGGCTGCAGGCCCACGATCGCTTGCT‑GATCCTGAAAAAACCGTTCGACAACATCGAGGTCCAGCAAATGGCCAGCACCTTGCTGACCAAGTGGGAAATGACCCAACGAGCCTCGCTGAAAAt                                                                                                    <  4:9795/149‑1 (MQ=255)                                                  cTGGGATGAGTTGCTCGACCGGCTGCAGGCCCACGATCGCTTGCT‑GCTCCTGAAAAAACCGTTCGACAACATCGAGGTCCAGCAAATGGCCAGCACCTTGCTGACCAAGTGGGAAATGACCCAACGAGCCTCGCTGTAAATGCACCAGc                                                                                            >  3:271322/1‑149 (MQ=255)                                                  cTGGGATGAGTTGCTCGACCGGCTGCAGGCCCACGATCGCTTGCT‑GATCCTGAAAAAACCGTTCGACAACATCGAGGTCCAGCAAATGGCCCGCACCTTGCTGACCAAGTGGGAAATGACCCAACGAGCCTCGCTGAAAATGCACCAGc                                                                                            >  7:134430/1‑149 (MQ=255)                                                  cTGGGATGAGTTGCTCGACCGGCTGCAGGCCCACGATCGCTTGCT‑GATCCTGAAAAAACCGTTCGACAACATCGAGGTCCAGCAAATGGCCAGCACCTTGCTGACCAAGTGGGAAATGACCCAACGAGCCTCGCTGAAAATGCACCAg                                                                                             <  8:117527/148‑1 (MQ=255)                                                  cTGGGATGAGTTGCTCGACCGGCTGCAGGCCCACGATCGCTTGCT‑GATCCTGAAAAAACCGTTCGACAACATCGAGGTCCAGCAAATGGCCAGCACCTTGCTGACCAAGTGGGAAATGACCCAACGAGCCTCGCTGAAAATGCACCAGc                                                                                            >  4:121747/1‑149 (MQ=255)                                                        tGAGTTGCTCGACCGGCTGCAGGCCCACTATCGCTTGCT‑GATCCTGAAAAAACCGTTCGACAACATCGAGGTCCATCAAATGGCCAGCACCTTGCTGACCAAGTGGGAAATGACCCAACGAGCCTCGCTGAAAATGCACCAGCTCGAAc                                                                                      <  7:110630/149‑1 (MQ=255)                                                              gCTCGACCGGCTGCAGGCCCACGATCGCTTGCT‑GATCCTGAAAAAACCGTTCGACAACATCGAGGTCCAGCAAATGGCCAGCACCTTGCTGACCAAGTGGGAAATGACCCAACGAGCCTCGCTGAAAATGCACCAGCTCGAACAGCgg                                                                                 <  3:17473/148‑1 (MQ=255)                                                              gCTCGAACGGCTGCAGGCCCACGATCGCTTGCT‑GATCCTGAAAAAACCGTTCGACAACATCGAGGTCCAGCAAATGGCCAGCACCTTGCTGACCAAGTGGGAAATGACCCAACGAGCCTCGCTGAAAATGCACCAGCTCGAACAGCgg                                                                                 <  3:21999/148‑1 (MQ=255)                                                                tCGACCGGCTGCAGGCCCACGATCGCTTGCTAGATCCTGAAAAAACCGTTCGACAACATCGAGGTCCAGCAAATGGCCAGCACATTGCTGACCAAGTGGGAAATGACCCAACGAGCCTCGCTGAAAATGCACCAGCTCGAACAGCggg                                                                                >  1:208450/1‑148 (MQ=255)                                                                  gACCGGCTGCAGGCCCACGATCGCTTGCT‑GATCCTGAAAAAACCGTTCGACAACATCGAGGTCCAGCAAATGGCCAGCACCTTGCTGCCGAAGTGGGAAATGACCCAACGAGCCTCGCTGAAAATGCACCAGCTCGAACAGCGGGTa                                                                              >  5:263045/1‑147 (MQ=255)                                                                  gACCGGCTGCAGGCCCACGATCGCTTGCT‑GATCCTGAAAAAACCGTTCGACAACATCGAGGTCCAGCAAATGGCCAGCACCTTGCTGACCAAGTGGGAAATGACCCAACGAGCCTCGCTGAAAATGCACCAGCTCGAACAGCGGGTa                                                                              >  4:139355/1‑147 (MQ=255)                                                                    ccGGCTGCAGGCCCACGATCGCTTGCT‑GATCCTGAAAAAACCGTTCGACAACATCGAGGTCCAGCAAATGGCCAGCACCTTGCTGACCAAGTGGGAAATGACCCAACGAGCCTCGCTGAAAATGCACCAGCTCGAACAGCGGGTAGAgc                                                                          <  3:121747/149‑1 (MQ=255)                                                                                ccACGATCGCTTGCT‑GATCCTGAAAAAACCGTTCGACAACATCGAGGTCCAGCAAATGGCCAGCACCTTGCTGACCAAGTGGGAAATGACCCAACGAGCCTCGCTGAAAATGCACCAGCTCGAACAGCGGGTAGAGCGGCGTACCCAg                                                               >  3:47771/1‑148 (MQ=255)                                                                                   cGATCGCTTGCT‑GATCCTGAAAAAACCGTTCGACAACATCGAGGTCCAGCAAATGGCCAGCACCTTGCTGACCAAGTGGGAAATGACCCAACGAGCCTCGCTGAAAATGCACCAGCTCGAACAGCGGGTAGAGCGGCGTACCCAGCAAc                                                           >  6:340072/1‑149 (MQ=255)                                                                                    gATCGCTTGCT‑GATCCTGAAAAAACCGTTCGACAACATCGAGGTCCAGCAAATGGCCAGCACCTTGCTGACCAAGTGGGAAATGACCCAACGAGCCTCGCTGAAAATGCACCAGCTCGAACAGCGGGTAGAGCGGCGTACCCAGCAACt                                                          >  5:26819/1‑149 (MQ=255)                                                                                    gATCGCTTGCT‑GATCCTGAAAAAACCGTTCGACAACATCGAGGTCCAGCAAATGGCCAGCACCTTGCTGACCAAGTGGGAAATGACCCAACGAGCCTCGCTGAAAATGCACCAGCTCGAACAGCGGGTAGAGCGGCGTACCCAGCAACt                                                          >  5:21839/1‑149 (MQ=255)                                                                                    gATCGCTTGCT‑GATCCTGAAAAAACCGTTCGACAACATCGAGGTCCAGCAAATGGCCAGCACCTTGCTGACCAAGTGGGAAATGACCCAACGAGCCTCGCTGAAAATGCACCAGCTCGAACAGCGGGTAGAGCGGCGTACCCAGCAACt                                                          >  3:107269/1‑149 (MQ=255)                                                                                        gCTTGCT‑GATCCTGAAAAAACCGTTCGACAACATCGAGGTCCAGCAAATGGCCAGCACCTTGCTGACCAAGTGGGAAATGACCCAACGAGCCTCGCTGAAAATGCACCAGCTCGAACAGCGGGTAGAGCGGCGTACCCAGCAACTGAc                                                       <  5:143382/148‑1 (MQ=255)                                                                                         cTTGCT‑GATCCTGAAAAAACCGTTCGACAACATCGAGGTCCAGCAAATGGCCAGCACCTTGCTGACCAAGTGGGAAATGACCCAACGAGCCTCGCTGAAAATGCACCAGCTCGAACAGCGGGTAGAGCGGCGTACCCAGCAACTGAcc                                                      >  5:338051/1‑148 (MQ=255)                                                                                          ttGCT‑GATCCTGAAAAAACCGTTCGACAACATCGAGGTCCAGCAAATGGCCAGCACCTTGCTGACCAAGTGGGAAATGACCCAACGAGCCTCGCTGAAAATGCACCAGCTCGAACAGCGGGTAGAGCGGCGTACCCAGCAACTGAcc                                                      >  7:86115/1‑147 (MQ=255)                                                                                            gCT‑GATCCTGATAAAACCTTTCGACAACATCGAGGTCCAGCAAATGGCAAGCACCTTGCTTACCAAGTGGGAAATGACCCTACGAGCCTCGCTGAAAATGCACCAGCTCGAACAGCGGGTAGAGCGGCGTACCCAGCAACTGACCCa                                                    <  8:189918/147‑1 (MQ=255)                                                                                             cT‑GATCCTGAAAAAACCGTTCGACAACATCGAGGTCCAGCAAATGGCCAGCACCTTGCTGACCAAGTGGGAAATGACCCAACGAGCCTCGCTGAAAATGCACCAGCTCGAACAGCGGGTAGAGCGGCGTACCCAGCAACTGACCCAGGc                                                 >  5:224895/1‑149 (MQ=255)                                                                                                 aTCCTGAAAAAACCGTTCGACAACATCGAGGTCCAGCAAATGGCCAGCACCTTGCTGACCAAGTGGGAAATGACCCAACGAGCCTCGCTGAAAATGCACCAGCTCGAACAGCGGGTAGAGCGGCGTACCCAGCAACTGACCCAGGCCAg                                              >  7:97745/1‑149 (MQ=255)                                                                                                     tGAAAAAACCGTTCGACAACATCGAGGTCCAGCAAATGGCCAGCACCTTGCTGACCAAGTGGGAAATGACCCAACGAGCCTCGCTGAAAATGCACCAGCTCGAACAGCGGGTAGAGCGGCGTACCCAGCAACTGACCCAGGCCAGCGAg                                          >  2:175546/1‑149 (MQ=255)                                                                                                        aaaaaCCGTTCGACAACATCGAGGTCCAGCAAATGGCCAGCACCTTGCTGACCAAGTGGGAAATGACCCAACGAGCCTCGCTGAAAATGCACCAGCTCGAACAGCGGGTAGAGCGGCGTACCCAGCAACTGACCCAGGCCAGCGAGgcg                                       <  4:47771/149‑1 (MQ=255)                                                                                                        aaaaaCCGTTCGACAACATCGAGGTCCAGCAAATGGCCAGCACCTTGCTGACCAAGTGGGAAATGACCCAACGAGCCTCGCTGAAAATGCACCAGCTCGAACAGCGGGTAGAGCGGCGTACCCAGCAACTGACCCAGGCCAGCGAGgcg                                       <  4:107269/149‑1 (MQ=255)                                                                                                         aaaaCCGTTCGACAACATCGAGGTCCAGCAAATGGCCAGCACCTTGCTGACCAAGTGGGAAATGACCCAACGAGCCTCGCTGAAAATGCACCAGCTCGAACAGCGGGTAGAGCGGCGTACCCAGCAACTGACCCAGGCCAGCGAGgcgc                                      >  5:141309/1‑149 (MQ=255)                                                                                                             ccGTTCGACAACATCGAGGTCCAGCAAATGGCCAGCACCTTGCTGACCAAGTGGGAAATGACCCAACGAGCCTCGCTGAAAATGCACCAGCTCGAACAGCGGGTAGAGCGGCGTACCCAGCAACTGACCCAGGCCAGCGAGGCGCTgca                                  <  6:21839/149‑1 (MQ=255)                                                                                                             ccGTTCGACAACATCGAGGTCCAGCAAATGGCCAGCACCTTGCTGACCAAGTGGGAAATGACCCAACGAGCCTCGCTGAAAATGCACCAGCTCGAACAGCGGGTAGAGCGGCGTACCCAGCAACTGACCCAGGCCAGCGAGGCGCTgca                                  <  6:26819/149‑1 (MQ=255)                                                                                                                  cGACAACATCGAGGTCCAGCAAATGGCCAGCACCTTGCTGACCAAGTGGGAAATGACCCAACGAGCCTCGCTGAAAATGCACCAGCTCGAACAGCGGGTAGAGCGGCGTACCCAGCAACTGACCCAGGCCAGCGAGGCGCTGcagcag                              <  6:338051/148‑1 (MQ=255)                                                                                                                      aacaTCGAGGTCCAGCAAATGGCCAGCACCTTGCTGACCAAGTGGGAAATGACACAACGAGCCTCGCTGAAAATGCACCAGCTCGAACAGCGGGTAGAGCGGCGTACCCAGCAACTGACCCAGGCCAGCGAGGCGCTGcagcag                              >  6:277752/1‑144 (MQ=255)                                                                                                                       acaTCGAGGTCCAGCAAATGGCCAGCACCTTGCTGACCAAGTGGGAAATGACACAACGAGCCTCGCTGAAAATGCACCAGCTCGAACAGCGGGTAGAGCGGCGTACCCAGCAACTGACCCAGGCCAGCGAGGCGCTGCAGCAGGAGATc                        <  5:277752/149‑1 (MQ=255)                                                                                                                          tCGAGGTCCAGCAAATGGCCAGCACCTTGCTGACCAAGTGGGAAATGACCCAACGAGCCTCGCTGAAAATGCACCAGCTCGAACAGCGGGTAGAGCGGCGTACCCAGCAACTGACCCAGGCCAGCGAGGCGCTGCAGCAGGAGATCgag                     <  7:285170/149‑1 (MQ=255)                                                                                                                                tCCAGCAAATGGCCAGCACCTTGCTGACCAAGTGGGAAATGACCCAACGAGCCTCGCTGAAAATGCACCAGCTCGAACAGCGGGTAGAGCGGCGTACCCAGCAACTGACCCAGGCCAGCGAGGCGCTGCAGCAGGAGATCGAGGAgcgc               <  8:86115/149‑1 (MQ=255)                                                                                                                                  cAGCAAATGGCCAGCACCTTGCTGACCAAGTGGGAAATGACCCAACGAGCCTCGCTGAAAATGCACCAGCTCGAACAGCGGGTAGAGCGGCGTACCCAGCAACTGACCCAGGCCAGCGAGGCGCTGCAGCAGGAGATCGAGGAGCGCaa             >  2:169539/1‑149 (MQ=255)                                                                                                                                        aTGGCCAGCACCTTGCTGACCAAGTGGGAAATGACCCAACGAGCCTCGCTGAAAATGCACCAGCTCGAACAGCGGGTAGAGCGGCGTACCCAGCAACTGACCCAGGCCAGCGAGGCGCTGCAGCAGGAGATCGAGGAGCGCAAGCAGCt       <  6:224895/149‑1 (MQ=255)                                                                                                                                           gCCAGCACCTTGCTGACCAAGTGGGAAATGACCCAACGAGCCTCGCTGAAAATGCACCAGCTCGAACAGCGGGTAGAGCGGCGTACCCAGCAACTGACCCAGGCCAGCGAGGCGCTGCAGCAGGAGATCGAGGAGCGCAAGCAGCTgg     <  1:167581/148‑1 (MQ=255)                                                                                                                                             cAGCACCTTGCTGACCAAGTGGGAAATGACCCAACGAGCCTCGCTGAAAATGCACCAGCTCGAACAGCGGGTAGAGCGGCGTACCCAGCAACTGACCCAGGCCAGCGAGGCGCTGCAGCAGGAGATCGAGGAGCGCAAGCAGCTGGaaa  <  5:281270/149‑1 (MQ=255)                                                                                                                                                   |                                                                                                                                               AGGACCCGCTGTTGCAGGTAGTCGTCTGCACTGCCTACTCGGACTATTCCTGGGATGAGTTGCTCGACCGGCTGCAGGCCCACGATCGCTTGCT‑GATCCTGAAAAAACCGTTCGACAACATCGAGGTCCAGCAAATGGCCAGCACCTTGCTGACCAAGTGGGAAATGACCCAACGAGCCTCGCTGAAAATGCACCAGCTCGAACAGCGGGTAGAGCGGCGTACCCAGCAACTGACCCAGGCCAGCGAGGCGCTGCAGCAGGAGATCGAGGAGCGCAAGCAGCTGGAAA  >  NC_002947/3349817‑3350104

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑