Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,352,168 A→G 6.3% intergenic (+58/+84) PP_2947 → / ← PP_2948 transcriptional regulator MvaT/GntR family transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,352,1680AG 6.3% 184.9 / 2.3 63intergenic (+58/+84)PP_2947/PP_2948transcriptional regulator MvaT/GntR family transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base A (26/33);  new base G (2/2);  total (28/35)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.93e-01

GGTCATCGAGACCAAGGGTGGCAACCACAAGCTGCTCAAGGAATGGAAAGCCCAGTACGGGGCAGAAGAAGTGGAATCCTGGGTGTCCTGATTTGTGCTGATGTCGCCCTCATTGCGCTATCTGAAACGCCTTGTTCTTTTTCGAGGAAGCGACAGCGTCGCCTCCTCGAAACAGGGCGTTCATCGTGGGTGGGCGGAATGAGCTGACGTGGTTAAACAGGAAGTTTCGCCACTAAGCGCTCAGTGTCCGTTTCAATACTTCGTAAATTTTGGCATCAGCCGATTGGGCGA  >  NC_002947/3352020‑3352310
                                                                                                                                                    |                                                                                                                                              
ggTCATCGAGACCAAGGGTGGCAACCACAAGCTGCTCAAGGAATGGAAAGCCCAGTACGGGGCAGAAGAAGTGGAATCCTGGGTGTCCTGATTTGTGCTGATGTCGCCCTCATTGCGCTATCTGAAACGCCTTGTTCTTTTTCGAGGaa                                                                                                                                                <  1:268762/149‑1 (MQ=255)
 gTCATCGAGACCAAGGGTGGCAACCACAAGCTGCTCAAGGAATGGAAAGCCCAGTACGGGGCAGAAGAAGTGGAATCCTGGGTGTCCTGATTTGTGCTGATGTCGCCCTCATTGCGCTATCTGAAACGCCTTGTTCTTTTTCGAGGAAg                                                                                                                                               <  8:2747/149‑1 (MQ=255)
       gagaCCAAGGGTGGCAACCACAAGCTGCTCAAGGAATGGAAAGCCCAGTACGGGGCAGAAGAAGTGGAATCCTGGGTGTCCTGATTTGTGCTGATGTCGCCCTCATTGCGCTATCTGAAACGCCTTGTTCTTTTTCGAGGAAGCGACa                                                                                                                                          <  2:179886/148‑1 (MQ=255)
        agaCCAAGGGTGGCAACCACAAGCTGCTCAAGGAATGGAAAGCCCAGTACGGGGCAGAAGAAGTGGAATCCTGGGTGTCCTGATTTGTGCTGATGTCGCCCTCATTGCGCTATCTGAAACGCCTTGTTATTTTTCGAGGAAGCGACAGc                                                                                                                                        >  5:267470/1‑149 (MQ=255)
          aCCAAGGGTGGCAACCACAAGCTGCTCAAGGAACGGAAAGCCCAGTACGGGGCAGAAGAAGTGGAATCCTGGGTGTCCTGATTTGTGCTGATGTCGCCCTCATTGCGCTATCTGAAACGCCTTGTTCTTTTTCGAGGAAGCGACAGCGt                                                                                                                                      <  4:149801/149‑1 (MQ=255)
           ccAAGGGTGGCAACCACAAGCTGCTCAAGGAATGGAAAGCCCAGTACGGGGCAGAAGAAGTGGAATCCTGGGTGTCCTGATTTGTGCTGATGTCGCCCTCATTGCGCTATCTGAAACGCCTTGTTCTTTTTCGAGGAAGCGACAGCGTc                                                                                                                                     >  4:253292/1‑149 (MQ=255)
                    gCAACCACAAGCTGCTCAAGGAATGGAAAGCCCAGTACGGGGCAGAAGAAGTGGAATCCTGGGTGTCCTGATTTGTGCTGATGTCGCCCTCATTGCGCTATCTGAAACGCCTTGTTCTTTTTCGAGGAAGCGACAGCGTCGCctcctc                                                                                                                             <  6:153937/148‑1 (MQ=255)
                      aaCCACAAGCTGCTCAAGGAATGGAAAGCCCAGTACGGGGCAGAAGAAGTGGAATCCTGGGTGTCCTGATTTGTGCTGATGTCGCCCTCATTGCGCTATCTGAAACGCCTTGTTCTGTTTAGAGGAGGCGACGCTGTCGCTTCCTCGa                                                                                                                           >  7:114938/1‑148 (MQ=255)
                        ccACAAGCTGCTCAAGGAATGGAAAGCCCAGTACGGGGCAGAAGAAGTGGAATCCTGGGTGTCCTGATTTGTGCTGATGTCGCCCTCATTGCGCTATCTGAAACGCCTTGTTCTTTTTCGAGGAAGCGACAGCGTCGCCTCCTCGaaa                                                                                                                         >  1:2496/1‑148 (MQ=255)
                          acaAGCTGCTCAAGGAATGGAAAGCCCAGTACGGGGCAGAAGAAGTGGAATCCTGGGTGTCCTGATTTGTGCTGATGTCGCCCTCATTGCGCTATCTGAAACGCCTTGTTCTTTTTCGAGGAAGCGACAGCGTCGCCTCCTCGAAACAg                                                                                                                      <  2:172733/149‑1 (MQ=255)
                          acaAGCTGCTCAAGGAATGGAAAGCCCAGTACGGGGCAGAAGAAGTGGAATCCTGGGTGGCCTGATTTGTGCTGATGTCGCCCTCATTGCGCTATCTGAAACGCCTTGTTCTTTTTCGAGGAAGCGACAGCGTCGCCTCCTCGAAACAg                                                                                                                      <  6:267470/149‑1 (MQ=255)
                           caAGGTGGTCAAGGAATGGAAAGCCCAGTACGGGGCAGAAGAAGTGGAATCCTGGGTGTCCTGATTTGTGCTGATGTGGCCCTCATTGCGCTATCTGAAACGCCTTGTTCTTTTTCGAGGAAGCGACAGCGTCGCCTCCTCGAAACAgg                                                                                                                     <  4:68031/149‑1 (MQ=255)
                           caAGCTGCTCAAGGAATGGAAAGCCCAGTACGGGGCAGAAGAAGTGGAATCCTGGGTGTCCTGATTTGTGCTGATGTCGCCCTCATTGCGCTATCTGAAACGCCTTGTTCTTTTTCGAGGAAGCGACAGCGTCGCCTCCTCGAAACAgg                                                                                                                     <  1:199810/149‑1 (MQ=255)
                             aGCTGCTCAAGGAATGGAAAGCCCAGTACGGGGCAGAAGAAGTGGAATCCTGGGTGTCCTGATTTGTGCTGATGTCGCCCTCATTGCGCTATCTGAAACGCCTTGTTCTTTTTCGAGGAAGCGACAGCGTCGCCTCCTCGAAACAGGGc                                                                                                                   <  2:73412/149‑1 (MQ=255)
                                  ctCAAGGTATGGAAAGCCCAGTACGTGGCAGAAGAAGTGGAATCGTGGGTGTCCTGATTTGTGCTGATGTCGCCCTCATTGCGCTATCTGAAACGCCTTGTTCTGTTTCGAGGAGGCGACGCTGTCGCTTCCTCGAAAAAgaacaag                                                                                                                <  8:114938/147‑7 (MQ=25)
                                  ctCAAGGAATGGAAAGCCCAGTACGGGGCAGAAGAAGTGGAATCCTGGGTGTCCTGATTTGTGCTGATGTCGCCCTCATTGCGCTATCTGAAACGCCTTGTTCTTTTTCGAGGAAGCGACAGCGTCGCCTCCTCGAAACAGGGCGTTc                                                                                                               <  8:273854/148‑1 (MQ=255)
                                   tCAAGGAATGGAAAGCCCAGTACGGGGCAGAAGAAGTGGAATCCTGGGTGTCCTGATTTGTGCTGATGTCGCCCTCATTGCGCTATCTGAAACGCCTTGTTCTTTTTCGAGGAAGCGACAGCGTCGCCTCCTCGAAACAGGGCGTTCa                                                                                                              >  8:159727/1‑148 (MQ=255)
                                   tCAAGGAATGGAAAGCCCAGTACGGGGCAGAAGAAGTGGAATCCTGGGTGTCCTGATTTGTGCTGATGTCGCCCTCATTGCGCTATCTGAAACGCCTTGTTCTTTTTCGAGGAAGCGACAGCGTCGCCTCCTCGAAACAGGGCGTTCAt                                                                                                             >  7:240986/1‑149 (MQ=255)
                                    cAAGGAATGGAAAGCCCAGTACGGGGCAGAAGAAGTGGAATCCTGGGTGTCCTGATTTGTGCTGATGTCGCCCTCATTGCGCTATCTGAAACGCCTTGTTCTTTTTCGAGGAAGCGACAGCGTCGCCTCCTCGAAACAGGGCGTTCATc                                                                                                            >  4:293734/1‑149 (MQ=255)
                                     aaGGAATGGAAAGCCCAGTACGGGGCAGAAGAAGTGGAATCCTGGGTGTCCTGATTTGTGCTGATGTCGCCCTCATTGCGCTATCTGAAACGCCTTGTTCTTTTTCGAGGAAGCGACAGCGTCGCCTCCTCGAAACAGGGCGTTCATc                                                                                                            <  7:159727/148‑1 (MQ=255)
                                      aGGAATGGAAAGCCCAGTACGGGGCAGAAGAAGTGGAATCCTGGGTGTCCTGATTTGTGCTGATGTCGCCCTCATTGCGCTATCTGAAACGCCTTGTTCTTTTTCGAGGAAGCGACAGCGTCGCCTCCTCGAAACAGGGCGTTCATCgt                                                                                                          >  8:194520/1‑149 (MQ=255)
                                        gAATGGAAAGCCCTGTACGGGGCAGAAGACGTGGAATCCTGGGTGTCCTGATTTGTGCTGATGTCGCCCTCATTGCGCTATCTGAAACGCCTTGTTCTTTTTCGAGGAAGCGACAGCGTCGCCTCCTCGAAACAGGGCGTTCATCgtgg                                                                                                        <  7:279116/149‑1 (MQ=255)
                                         aaTGGAAAGCCCAGTACGGGGCAGAAGAAGTGGAATCCTGGGTGTCCTGATTTGTGCTGATGTCGCCCTCATTGCGCTATCTGAAACGCCTTGTTCTTTTTCGAGGAAGCGACAGCGTCGCCTCCTCGAAACAGGGCGTTCATCgtgg                                                                                                        <  3:82679/148‑1 (MQ=255)
                                             gAAAGCCCAGTACGGGGCAGAAGAAGTGGTATCCTGGGTGTCCTGAGTTGTGCTGATGTCGCGCTCATTGCGCTATCTGAAACGCCTTGTGCTTTTTCGAGGAAGCGACAGCGTCTCCTCCTCGAAACAGGGCGTTCATCgtgggtgg                                                                                                    <  3:164761/148‑1 (MQ=255)
                                              aaaGCCCAGTACGGGGCAGAAGAAGTGGAATCCTGGGTGTCCTGATTTGTGCTGATGTCGCCCTCATTGCGCTATCTGAAACGCCTTGTTCTTTTTCGAGGAAGCGACAGCGTCGCCTCCTCGAAACAGGGCGTTCATCGTGGGTGGGc                                                                                                  >  3:200420/1‑149 (MQ=255)
                                              aaaGCCCAGTACGGGGCAGAAGAAGTGGAATCCTGGGTGTCCTGATTTGTGCTGATGTCGCCCTCATTGCGCTATCTGAAACGCCTTGTTCTTTTTCGAGGAAGCGACAGCGTCGCCTCCTCGAAACAGGGCGTTCATCGTGGGTGGGc                                                                                                  >  1:206628/1‑149 (MQ=255)
                                                  cccAGTACGGGGCAGAAGAAGTGGAATCCTGGGTGTCCTGATTTGTGCTGATGTCGCCCTCATTGCGCTATCTGAAACGCCTTGTTCTTTTTCGAGGAAGCGACAGCGTCGCCTCCTCGAAACAGGGCGTTCATCGTGGGTGGGCGGaa                                                                                              <  7:231877/149‑1 (MQ=255)
                                                  cccAGTACGGGGCAGAAGAAGTGGAATCCCGGGTGTCCTGATTTGTGCTGATGTCGCCCTCATTGCGCTATCTGAAACGCCTTGTTCTTTTTCGAGGAAGCGACAGCGTCGCCTCCTCGAAACAGGGCGTTCATCGTGGGTGGGCGGat                                                                                              <  5:302879/149‑2 (MQ=255)
                                                   ccAGTACGGGGCAGAAGAAGTGGAATCCTGGGTGTCCTGATTTGTGCTGATGTCGCCCTCATTGCGCTATCTGAAACGCGTTGTTCTTTTTCGAGGAAGCGACAGCGTCGCCTCCTCGAAACAGGGCGTTCATCGTGGGTGGGCGGAAt                                                                                             >  8:79107/1‑149 (MQ=255)
                                                      gTACGGGGCAGAAGAAGTGGAATCCTGGGTGTCCTGATTTGTGCTGATGTCGCCCTCATTGCGCTATCTGAAACGCCTTGTTCTTTTTCGAGGAAGCGACAGCGTCGCCTCATCGAAACAGGGCGTTCATCGTGGGTGGGCGGAATGAg                                                                                          <  4:301442/149‑1 (MQ=255)
                                                               agaagaAGTGGAATCCTGGGTGTCCTGATTTGTGCTGATGTCGCCCTCATTGCGCTATCTGAAACGCCTTGTTCTTTTTCGAGGAAGCGACAGCGTCGCCTCCTCGAAACAGGGCGTTCATCGTGGGTGGGCGGAATGAGCTGACGTgg                                                                                 >  6:314723/1‑149 (MQ=255)
                                                                   gaagTGGAATCCTGGGTGTCCTGATTTGTGCTGATGTCGCCCTCATTGCGCTATCTGAAACGCCTTGTTCTTTTTCGAGGAAGCGACAGCGTCGCCTCCTCGAAACAGGGCGTTCATCGTGGGTGGGCGGAATGAGCTGACg                                                                                    >  1:182706/1‑142 (MQ=255)
                                                                   gaagTGGAATCCTGGGTGTCCTGATTTGTGCTGATGTCGCCCTCATTGCGCTATCTGAAACGCCTTGTTCTTTTTCGAGGAAGCGACAGCGTCGCCTCCTCGAAACAGGGCGTTCATCGTGGGTGGGCGGAATGAGCTGACg                                                                                    <  2:182706/142‑1 (MQ=255)
                                                                     agTGGAATCCTGGGTGTCCTGATTTGTGCTGATGTCGCCCTCATTGCGCTATCTGAAACGCCTTGTTCTTTTTCGAGGAGGCGACGCTGTCGcttcct                                                                                                                              >  8:194660/1‑98 (MQ=38)
                                                                     agTGGAATCCTGGGTGTCCTGATTTGTGCTGATGTCGCCCTCATTGCGCTATCTGAAACGCCTTGTTCTTTTTCGAGGAGGCGACGCTGTCGcttcct                                                                                                                              <  7:194660/98‑1 (MQ=38)
                                                                          aaTCCTGGGTGTCCTGATTTGTGCTGATGTCGCCCTCATTGCGCTATCTGAAACGCCTTGTTCTTTTTCGAGGAAGCGACAGCGTCGCCTCCTCGAAACAGGGCGTTCATCGTGGGTGGGCGGAATGAGCTGACGTGGTTAAACAGGaa                                                                      <  7:44173/149‑1 (MQ=255)
                                                                                ggttgtgCTGCTTTGTGCTGATGTCGACCTCATTTCGCTATCTGAAAGGCCTTGTTCTTTTTCGAGGAAGCGACAGCGTCGCCTCCTCGAAACAGGGCGTTCATCGTGGGTGGGCGGAATGAGCTGACGTGGTTAAACAGGAAGTTTc                                                                 <  4:209874/145‑1 (MQ=255)
                                                                                   tgtCCTGATTTGTGCTGATGTCGCCCTCATTGCGCTATCTGAAACGCCTTGTTCTTTTTCGAGGAAGCGACAGCGTCGCCTCCTCGAAACAGGGCGTTCATCGTGGGTGGGCGGAATGAGCTGACGTGGTTAAACAGGAAGTTTCGCCa                                                             >  2:234643/1‑149 (MQ=255)
                                                                                       cTGATTTGTGCTGATGTCGCCCTCATTGCGCTATCTGAAACGCCTTGTTCTTTTTCGAGGAAGCGACAGCGTCGCCTCCTCGAAACAGGGCGTTCATCGTGGGTGGGCGGAATGAGCTGACGTGGTTAAACAGGAAGTTTCGCCACTac                                                         >  2:297335/1‑148 (MQ=255)
                                                                                       cTGATTTGTGCTGATATCGCCCTCATTGCGCTATCTGAAACGCCTTGTTCTTTTTCGAGGAAGCGACAGCGTCGCCTCCTCGAAACAGGGCGTTCATCGTGGGTGGGCGGAATGAGCTGACGTGGTTAAACAGGAAGTTTCGCCACt                                                           >  3:57605/1‑147 (MQ=255)
                                                                                        tGATTTGTGCTGATGTCGCCCTCATTGCGCTATCTGAAACGCCTTGTTCTTTTTCGAGGAAGCGACAGCGTCGCCTCCTCGAAACAGGGCGTTCATCGTGGGTGGGCGGAATGAGCTGACGTGGTTAAACAGGAAGTTTCGCCACTAAg                                                        >  8:307644/1‑149 (MQ=255)
                                                                                         gATTTGTGCTGATGTCGCCCTCATTGCGCTATCTGAAACGCCTTGTTCTTTTTCGAGGAAGCGACAGCGTCGCCTCCTCGAAACAGGGc                                                                                                                   <  8:43379/89‑1 (MQ=255)
                                                                                         gATTTGTGCTGATGTCGCCCTCATTGCGCTATCTGAAACGCCTTGTTCTTTTTCGAGGAAGCGACAGCGTCGCCTCCTCGAAACAGGGc                                                                                                                   >  7:43379/1‑89 (MQ=255)
                                                                                               tgCTGATGTCGCCCTCATTGCGCTATCTGAAACGCCTTGTTCTTTTTCGAGGAAGCGACAGCGTCGCCTCCTCGAAACAGGGCGTTCATCGTGGGTGGGCGGAATGAGCTGACGTGGTTAAACAGGAAGTTTCGCCACTAAGCGCTCa                                                  <  3:253292/148‑1 (MQ=255)
                                                                                               tgCTGATGTCGCCCTCATTGCGCTATCTGAAACGCCTTGTTCTTTTTCGAGGAAGCGACAGCGTCGCCTCCTCGAAACAGGGCGTTCATCGTGGGTGGGCGGAATGAGCTGACGTGGTTAAACAGGAAGTTTCGCCACTAAGCGCTCa                                                  <  8:240986/148‑1 (MQ=255)
                                                                                               tgCTGATGTCGCCCTCATTGCGCTATCTGAAACGCCTTGTTCTTTTTCGAGGAAGCGACAGCGTCGCCTCCTCGAAACAGGGCGTTCATCGTGGGTGGGCGGAATGAGCTGACGTGGTTAAACAGGAAGTTTCGCCACTAAGCGCTCa                                                  <  2:2496/148‑1 (MQ=255)
                                                                                                  tGATGTCGCCCTCATTGCGCTATCTGAAACGCCTTGTTCTTTTTCGAGGAAGCGACAGCGTCGCCTCCTCGAAACAGGGCGTTCATCGTGGGTGGGCGGAATGAGCTGACGTGGTTAAACAGGAAGTTTCGCCACTAAGCGCTCAgtgt                                              >  3:155506/1‑149 (MQ=255)
                                                                                                  tGATGTCGCCCTCATTGCGCTATCTGAAACGCCTTGTTCTTTTTCGAGGAAGCGACAGCGTCGCCTCCTCGAAACAGGGCGTTCATCGTGGGTGGGCGGAATGAGCTGACGTGGTTAAACAGGAAGTTTCGCCACTAAGCGCTCAgtgt                                              >  4:174497/1‑149 (MQ=255)
                                                                                                    aTGTCGCCCTCATTGCGCTATCTGAAACGCCTTGTTCTTTTTCGAGGAAGCGACAGCGTCGCCTCCTCGAAACAGGGCGTTCATCGTGGGTGGGCGGAATGAGCTGACGTGGTTAAACAGGAAGTTTCGCCACTAAGCGCTCAGTGTcc                                            <  1:297335/149‑1 (MQ=255)
                                                                                                         gCCCTCATTGCGCTATCTGAAACGCCTTGTTCTTTTTCGAGGAAGCGACAGCGTCGCCTCCTCGAAACAGGGCGTTCATCGTGGGTGGGCGGAATGAGCTGACGTGGTTAAACAGGAAGTTTCGCCACTAAGCGCTCAGTGTCCGttt                                        >  7:306911/1‑148 (MQ=255)
                                                                                                         gCCCTCATTGCGCTATCTGAAACGCCTTGTTCTTTTTCGAGGAAGCGACAGCGTCGCCTCCTCGAAACAGGGCGTTCATCGTGGGTGGGCGGAATGAGCTGACGTGGTTAAACAGGAAGTTTCGCCACTAAGCGCTCAGTGTCCGTTTc                                       <  3:174497/149‑1 (MQ=255)
                                                                                                           ccTCATTGCGCTATCTGAAACGCCTTGTTCTTTTTCGAGGAAGCGACAGCGTCGCCTCCTCGAAACAGGGCGTTCATCGTGGGTGGGCGGAATGAGCTGACGTGGTTAAACAGGAAGTTTCGCCACTAAGCGCTCAGTGTCCGTTTCaa                                     >  1:321735/1‑149 (MQ=255)
                                                                                                                  gcgcTATCTGAAACGCCTTGTTCTTTTTCGAGGAAGCGACAGCGTCGCCTCCTCGAAACAGGGCGTTCATCGTGGGTGGGCGGAATGAGCTGACGGGGTTAAACAGGAAGGTTCGCCACTAAGCGCTCAGTGTCCGTTTCAATACTTc                               <  2:84752/148‑1 (MQ=255)
                                                                                                                    gcTATCTGAAACGCCTTGTTCTTTTTCGAGGAAGCGACAGCGTCGCCTCCTCGAAACAGGGCGTTCATCGTGGGTGGGCGGAATGAGCTGACGTGGTTAAACAGGAAGTTTCGCCACTAAGCGCTCAGTGTCCGTTTCAATACTTc                               >  8:160176/1‑146 (MQ=255)
                                                                                                                    gcTATCTGAAACGCCTTGTTCTTTTTCGAGGAAGCGACAGCGTCGCCTCCTCGAAACAGGGCGTTCATCGTGGGTGGGCGGAATGAGCTGACGTGGTTAAACAGGAAGTTTCGCCACTAAGCGCTCAGTGTCCGTTTCAATACTTc                               <  7:160176/146‑1 (MQ=255)
                                                                                                                     cTATCTGAAACGCCTTGTTCTTTTTCGAGGAAGCGACAGCGTCGCCTCCTCGAAACAGGGCGTTCATCGTGGGTGGGCGGAATGAGCTGACGTGGTTAAACAGGAAGTTTCGCCACTaa                                                         <  6:240336/119‑1 (MQ=255)
                                                                                                                     cTATCTGAAACGCCTTGTTCTTTTTCGAGGAAGCGACAGCGTCGCCTCCTCGAAACAGGGCGTTCATCGTGGGTGGGCGGAATGAGCTGACGTGGTTAAACAGGAAGTTTCGCCACTaa                                                         >  5:240336/1‑119 (MQ=255)
                                                                                                                        tCTGAAACGCCTTGTTCTTTTTCGAGGAAGCGACAGCGTCGCCTCCTCGAAACAGGGCGTTCATCGTGGGTGGGCGGAATGAGCTGACGTGGTTAAACAGGAAGTTTCGCCACTAAGCGCTCAGTGTCCGTTTCAATACTTCGTAAAt                         <  1:234643/148‑1 (MQ=255)
                                                                                                                           gAAACGCCTTGTTCTTTTTCGAGGAAGCGACAGCGTCGCCTCCTCGAAACAGGGCGTTCATCGTGGGTGGGCGGAATGAGCTGACGTGGTTAAACAGGAAGTTTCGCCACTAAGCGCTCAGTGTCCGTTTCAATACTTCGTAAATTTTg                     >  6:241971/1‑149 (MQ=255)
                                                                                                                            aaaCGCCTTGTTCTTTTTCGAGGAAGCGACAGCGTCGCCTCCTCGAAACAGGGCGTTCATCGTGGGTGGGCGGAATGAGCTGACGTGGTTAAACAGGAAGTTTCGCCACTAAGCGCTCAGTGTCCGTTTCAATACTTCGTAAAtttt                      <  7:194520/147‑1 (MQ=255)
                                                                                                                                 ccTTGTTCTTTTTCGAGGAAGCGACAGCGTCGCCTCCTCGAAACAGGGCGTTCATCGTGGGTGGGCGGAATGAGCTGACGTGGTTAAACAGGAAGTTTCGCCACTAAGCGCTCAGTGTCCGTTTCAATACTTCGTAAATTTTGGCATCa               <  4:200420/149‑1 (MQ=255)
                                                                                                                                  cTTGTTCTTTTTCGAGGAAGCGACAGCGTCGCCTCCTCGAAACAGGGCGTTCATCGTGGGTGGGCGGAATGAGCTGACGTGGTTAAACAGGAAGTTTCGCCACTAAGCGCTCAGTGTCCGTTTCAATACTTCGTAAATTTTGGCATCAg              >  5:127317/1‑149 (MQ=255)
                                                                                                                                     gTTCTTTTTCGAGGAAGCGACAGCGTCGCCTCCTCGAAACAGGGCGTTCATCGTGGGTGGGCGGAATGAGCTGACGTGGTTAAACAGGAAGTTTCGCCACTAAGCGCTCAGTGTCCGTTTCAATACTTCGTAAATTTTGGCATCAGCCg           >  5:127330/1‑149 (MQ=255)
                                                                                                                                           tttCGAGGAAGCGACAGCGTCGCCTCCTCGAAACAGGGCGTTCATCGTGGGTGGGCGGAATGAGCTGACGTGGTTAAACAGGAAGTTTCGCCACTAAGCGCTCAGTGTCCGTTTCAATACTTCGTAAATTTTGGCATCAGCCGATTgg      <  3:293734/148‑1 (MQ=255)
                                                                                                                                               gaggagGCGACGCTGTCGCTTCCTCGAAACAGGGCGTTCATCGTGGGTGGGCGGAATGAGCTGACGTGGTTAAACAGGAAGTTTCGCCACTAAGCGCTCAGTGTCCGTTTCAATACTTCGTAAATTTTGGCATCAGCCGATTGGGCGa  >  5:63693/1‑148 (MQ=255)
                                                                                                                                                    |                                                                                                                                              
GGTCATCGAGACCAAGGGTGGCAACCACAAGCTGCTCAAGGAATGGAAAGCCCAGTACGGGGCAGAAGAAGTGGAATCCTGGGTGTCCTGATTTGTGCTGATGTCGCCCTCATTGCGCTATCTGAAACGCCTTGTTCTTTTTCGAGGAAGCGACAGCGTCGCCTCCTCGAAACAGGGCGTTCATCGTGGGTGGGCGGAATGAGCTGACGTGGTTAAACAGGAAGTTTCGCCACTAAGCGCTCAGTGTCCGTTTCAATACTTCGTAAATTTTGGCATCAGCCGATTGGGCGA  >  NC_002947/3352020‑3352310

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑