Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,463,619 G→A 7.4% P247S (CCA→TCA)  PP_3078 ← ABC transporter substrate‑binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,463,6190GA 7.4% 155.8 / 2.1 55P247S (CCA→TCA) PP_3078ABC transporter substrate‑binding protein
Reads supporting (aligned to +/- strand):  ref base G (23/27);  new base A (2/2);  total (26/29)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.59e-01

CCACTTCGGTGTCGCCGAAGTACTCGATGCGCAGTTTGTCGAAATTGTAGCGGCCACGGCTGGCGGGCAGGTCACGTGCCCACCATGCCGGGTCGCGTTCGAAGGTGATGCTGCGGCCGTTGTCG‑ATG‑‑CGCCCAATGCGGT‑ATG‑GCCCGCTGCCGACTGGCTTGTCGAAGCCGGCACCGTTG‑GCAAAGTCGCGCTGCTGCAGGTCGTGCTCGGGCATTACCGGCAGGCTGGCGAGGTCCAGCGCCAGGGTGCGGCCGTGCTCGGGCTTCAAGTCGAATCGCACGCTGTGCAG  >  NC_002947/3463475‑3463766
                                                                                                                                                     |                                                                                                                                                    
ccACTTCGGTGTCGCCGAAGTACTCGATGCGCAGTTTGTCGAAATTGTAGCGGCCACGGCTGGCGGGCAGGTCACGTGCCCACCATGCCGGGTCGCGTTCGAAGGTGATGCTGCGGCCGTTGTCG‑ATG‑‑CGCCCAATGCGGT‑ATG‑GCCCg                                                                                                                                                  <  8:19038/149‑1 (MQ=255)
 cACTTCGGTGTCGCCGAAGAACTCGATGCGCAGTTTGTCGAAATTGTAGCGGCCACGGCTGGCGGGCAGGTCACGTGCCCACCATGCCGGGTCGCGTTCGAAGGTGATGCTGCGGCCGTTGTCG‑ATG‑‑CGCCCAATGCGGT‑ATG‑GCCCGc                                                                                                                                                 <  4:110567/149‑1 (MQ=255)
  aCTTCGGTGTCGCCGAAGTACTCGATGCGCAGTTTGTCGAAATTGTAGCGGCCACGGCTGGCGGGCAGGTCACGTGCCCACCATGCCGGGTCGCGTTCGAAGGTGATGCTGCGGCCGTTGTCG‑ATG‑‑CGCCCAATGCGGT‑ATG‑GCCCGCt                                                                                                                                                <  2:104671/149‑1 (MQ=255)
        gtgtCGCCGAAGTACTCGATGCGCAGTTTGTCGAAATTGTAGCGGCCACGGCTGGCGGGCAGGTCACGTGCCCACCATGCCGGGTCGCGTTCGAAGGTGATGCTGCGGCCGTTGTCG‑ATG‑‑CGCCCAATGCGGT‑ATG‑GCCCGCTGCCGAc                                                                                                                                          <  1:22094/149‑1 (MQ=255)
        gtgtCGCCGAAGTACTCGATGCGCAGTTTGTCGAAATTGTAGCGGCCACGGCTGGCGGGCAGGTCACGTGCCCACCATGCCGGGTCGCGTTCGAAGGTGATGCTGCGGCCGTTGTCG‑ATG‑‑CGCCCAATGCGGT‑ATG‑GCCCGCTGCCGAc                                                                                                                                          <  6:275639/149‑1 (MQ=255)
        gtgtCGCCGAAGTACTCGATGCGCAGTTTGTCGAAATTGTAGCGGCCACGGCTGGCGGGCAGGTCACGTGCCCACCATGCCGGGTCGCGTTCGAAGGTGATGCTGCGGCCGTTGTCG‑ATG‑‑CGCCCAATGCGGT‑ATG‑GCCCGCTGCCGAc                                                                                                                                          <  7:143499/149‑1 (MQ=255)
             gCCGAAGTACTCGATGCGCAGTTTGTCGAAATTGTAGCGGCCACGGCTGGCGGGCAGGTCACGTGCCCACCATGCCGGGTCGCGTTCGAAGGTGATGCTGCGGCCGTTGTCG‑ATG‑‑CGCCCAATGCGGT‑ATG‑GCCCGCTGCCGACTGGCt                                                                                                                                     >  5:50304/1‑149 (MQ=255)
             gCCGAAGTACTCGATGCGCAGTTTGTCGAAATTGTAGCGGCCACGGCTGGCGGGCAGGTCACGTGCCCACCATGCCGGGTCGCGTTCGAAGGTGATGCTGCGGCCGTTGTCG‑ATG‑‑CGCCCAATGCGGT‑ATG‑GCCCGCTGCCGACTGGCt                                                                                                                                     >  6:242990/1‑149 (MQ=255)
                gAAGTACTCGATGCGCAGTTTGTCGAAATTGTAGCGGCCACGGCTGGCGGGCAGGTCACGTGCCCACCATGCCGGGTCGCGTTCGAAGGTGATGCTGCGGCCGTTGTCG‑ATG‑‑CGCCCAATGCGGT‑ATG‑GCCCGCTGCCGACTGGCTTGt                                                                                                                                  >  2:149890/1‑149 (MQ=255)
                 aaGTACTCGATGCGCAGTTTGTCGAAATTGTAGCGGCCACGGCTGGCGGGCAGGTCACGTGCCCACCATGCCGGGTCGCGTTCGAAGGTGATGCTGCGGCCGTTGTCG‑ATG‑‑CGCCCAATGCGGT‑ATG‑GCCCGCt                                                                                                                                                >  1:130006/1‑134 (MQ=255)
                 aaGTACTCGATGCGCAGTTTGTCGAAATTGTAGCGGCCACGGCTGGCGGGCAGGTCACGTGCCCACCATGCCGGGTCGCGTTCGAAGGTGATGCTGCGGCCGTTGTCG‑ATG‑‑CGCCCAATGCGGT‑ATG‑GCCCGCt                                                                                                                                                <  2:130006/134‑1 (MQ=255)
                         gATGCGCAGTTTGTCGAAATTGTAGCGGCCACGGCTGGCGGGCAGGTCACGTGCCCACCATGCCGGGTCGCGTTCGAAGGTGATGCTGCGGCCGTTGTCG‑ATG‑‑CGCCCAATGCGGT‑ATG‑GCCCGCTGCCGACTGGCTTGTCGAAGCCgg                                                                                                                         <  4:174402/149‑1 (MQ=255)
                         gATGCGCAGTTTGTCGAAATTGTAGCGGCCACGGCTGGCGGGCAGGTCACGTGCCCACCATGCCGGGTCGCGTTCGAAGGTGATGCTGCGGCCGTTGTCG‑ATG‑‑CGCCCAATGCGGT‑ATG‑GCCCGCTGCCGACTGGCTTGTCGAAGCCgg                                                                                                                         >  8:124611/1‑149 (MQ=255)
                         gATGCGCAGTTTGTCGAAATTGTAGCGGCCACGGCTGGCGGGCAGGTCACGTGCCCACCATGCCGGGTCGCGTTCGAAGGTGATGCTGCGGCCGTTGTCG‑ATG‑‑CGCCCAATGCGGT‑ATG‑GCCCGCTGCCGACTGGCTTGTCGAAGCCgg                                                                                                                         >  5:9333/1‑149 (MQ=255)
                               cAGTTTGTCGAAATTGTAGCGGCCACGGCTGGCGGGCAGGTCACGTGCCCACCATGCCGGGTCGCGTTCGAAGGTGATGCTGCGGCCGTTGTCG‑ATG‑‑CGCCCAATGCGGT‑ATG‑GCCCGCTGCCGACTGGCTTGTCGAAGCCGGCACCGt                                                                                                                   <  7:124611/149‑1 (MQ=255)
                                  tttGTCGAAATTGTAGCGGCCACGGCTGGCGGGCAGGTCACGTGCCCACCATGCCGGGTCGCGTTCGAAGGTGATGCTGCGGCCGTTGTCG‑ATG‑‑CGCCCAATGCGGT‑ATG‑GCCCGCTGCCGACTGGCTTGTCGAAGCCGGCACCGTTg‑g                                                                                                               >  6:263969/1‑149 (MQ=255)
                                        gAAATTGTAGCGGCCACGGCTGGCGGGCAGGTCACGTGCCCACCATGCCGGGTCGCGTTCGAAGGTGATGCTGCGGCCGTTGTCG‑ATG‑‑CGCCCAATGCGGT‑ATG‑GCCCGCTGCCGCCTGGCTTGTCGAAGCCGGCACCGTTG‑GCAAAGt                                                                                                         >  6:168791/1‑149 (MQ=255)
                                          aaTTGTAGCGGCCACGGCTGGCGGGCAGGTCACGTGCCCACCATGCCGGGTCGCGTTCGAAGGTGATGCTGCGGCCGTTGTCG‑ATG‑‑CGCCCAATGCGGT‑ATG‑ACCCGCTGCCGACTGGCTTGTCGAAGCCGGCACCGTTG‑GCAAAGTcg                                                                                                       >  7:198032/1‑149 (MQ=255)
                                          aaTTGTAGCGGCCACGGCTGGCGGGCAGGTCACGTGCCCACCATGCCGGGTCGCGTTCGAAGGTGATGCTGCGGCCGTTGTCG‑ATG‑‑CGCCCAATGCGGT‑ATG‑ACCCGCTGCCGACTGGCTTGTCGAAGCCGGCACCGTTG‑GCAAAGTcg                                                                                                       >  7:304075/1‑149 (MQ=255)
                                           aTTGTAGCGGCCACGGCTGGCGGGCAGGTCACGTGCCCACCATGCCGGGTCGCGTTCGAAGGTGATGCTGCGGCCGTTGTCG‑ATG‑‑CGCCCAATGCGGT‑ATG‑GCCCGCTGCCGACTGGCTTGTCGAAGCCGGCACCGTTG‑GCAAAGTcgc                                                                                                      <  6:50304/149‑1 (MQ=255)
                                                 gCGGCCACGGCTGGCGGGCAGGTCACGTGCCCACCATGCCGGGTCGCGTTCGAAGGTGATGCTGCGGCCGTTGTCG‑ATG‑‑CGCCCAATGCGGT‑ATG‑GCCCGCTGCCGACTGGCTTGTCGAAGCCGGCACCGTTG‑GCAAAGTCGCgctgct                                                                                                <  1:149890/149‑1 (MQ=255)
                                                   ggCCACGGCTGGCGGGCAGGTCACGTGCCCACCATGCCGGGTCGCGTTCGAAGGTGATGCTGCGGCCGTTGTCG‑ATG‑‑CGCCCAATGCGGT‑ATG‑GCCCGCTGCCGACTGGCTTGTCGAAGCCGGCACCGTTG‑GCAAAGTCGCGCtgctgc                                                                                              <  4:91740/149‑1 (MQ=255)
                                                    gCCACGGCTGGCGGGCAGGTCACGTGCCCACCATGCCGGGTCGCGTTCGAAGGTGATGCTGCGGCCGTTGTCG‑ATG‑‑CGCCCAATGCGGT‑ATG‑GTCCGCTGCCGACTGGCTTGTCGAAGCCGGCACCGTTG‑GCAAAGTCGCGctgctg                                                                                               >  6:178865/1‑147 (MQ=255)
                                                     ccACGGCTGGCGGGCAGGTCACGTGCCCACCATGCCGGGTCGCGTTCGAAGGTGATGCTGCGGCCGTTGTCG‑ATG‑‑CGCCCAATGCGGT‑ATG‑GCCCGCTGCCGACTGGCTTGTCGAAGCCGGCACCGTTG‑GCAAAGTCGCGCTGCTGCAg                                                                                            >  5:183265/1‑149 (MQ=255)
                                                     ccACGGCTGGCGGGCAGGTCACGTGCCCACCATGCCGGGTCGCGTTCGAAGGTGATGCTGCGGCCGTTGTCG‑ATG‑‑CGCCCAATGCGGT‑ATG‑GCCCGCTGCCGACTGGCTTGTCGAAGCCGGCACCGTTG‑GCAAAGTCGCGCTGCTGCAg                                                                                            >  2:63710/1‑149 (MQ=255)
                                                      cACGGCTGGCGGGCAGGTCACGTGCCCACCATGCCGGGTCGCGTTCGAAGGTGATGCTGCGGCCGTTGTCG‑ATG‑‑CGCCCAATGCGGT‑ATG‑GCCCGCTGCCGACTGGCTTGTCGAAGCCGGCACCGTTG‑GCAAAGTCGCGCTGCTGCAgg                                                                                           >  8:151292/1‑149 (MQ=255)
                                                       aCGGCTGGCGGGCAGGTCACGTGCCCACCATGCCGGGTCGCGTTCGAAGGTGATGCTGCGGCCGTTGTCGAATGGAGGCCCAATGCGGTGATGCCCCCGCAGCCGAGTGTCTTGTCGAAGCCCGC‑CGGTTGCAAAAAGTCGCTctgctg                                                                                               >  1:276112/1‑149 (MQ=255)
                                                         ggCTGGCGGGCAGGTCACGTGCCCACCATGCCGGGTCGCGTTCGAAGGTGATGCTGCGGCCGTTGTCG‑ATG‑‑CGCCCAATGCGGT‑ATG‑GCCCGCTGCCGAc                                                                                                                                          <  6:294604/100‑1 (MQ=255)
                                                          gCTGGCGGGCAGGTCACGTGCCCACCATGCCGGGTCGCGTTCGAAGGTGATGCTGCGGCCGTTGTCG‑ATG‑‑CGCCCAATGCGGT‑ATG‑GCCCGCTGCCGACTGGCTTGTCGAAGCCGGCACCGTTG‑GCAAAGTCTCGCTGCTGCAGGTCGt                                                                                       <  1:63710/149‑1 (MQ=255)
                                                            tggtggGCACGTGACCTGCCCGCCATGCCGGGTCGCGTTCGAAGGTGATGCTGCGGCCGTTGTCG‑ATG‑‑CGCCCAATGCGGT‑ATG‑GCCCGCTGCCGACTGGCTTGTCGAAGCCGGCACCGTTG‑GCAAAGTCGCGCTGCTGCAGGTCGTGc                                                                                     >  1:73247/1‑149 (MQ=255)
                                                              gCGGGCAGGTCACGTGCCCACCATGCCGGGTCGCGTTCGAAGGTGATGCTGCGGCCGTTGTCG‑ATG‑‑CGCCCAATGCGGT‑ATG‑GCCCGCTGCCGACTGGCTTGTCGAAGCCGGCACCGTTG‑GCAAAGTCGCGCTGCTGCAGGTCGTGCt                                                                                    <  2:116318/148‑1 (MQ=255)
                                                               cGGGCAGGTCACGTGCCCACCATGCCGGGTCGCGTTCGAAGGTGATGCTGCGGCCGTTGTCG‑ATG‑‑CGCCCAATGCGGT‑ATG‑GCCCGCTGCCGACTGGCTTGTCGAAGCCGGCACCGTTG‑GCAAAGTCGCGCTGCTGCAGGTCGTGCTCg                                                                                  <  4:126786/149‑1 (MQ=255)
                                                                gggCAGGTCACGTGCCCACCAACACGGGTCGCGTTCGAAGGTCATGCTCCGGCACTTGTCC‑ATG‑‑CGCCCAATGCGGT‑ATG‑GCCAGCTGCCGACTGGCTTGTCGAAGCCGCCACCgaag                                                                                                                 >  6:316017/1‑115 (MQ=255)
                                                                     ggTCACGTGCCCACCATGCCGGGTCGCGTTCGAAGGTGATGCTGCGGCCGTTGTCG‑ATG‑‑CGCCCAATGCGGT‑ATG‑GCCCGCTGCCGACTGGCTTGTCGAAGCCGGCACCGTTG‑GCAAAGTCGCGCTGCTGCAGGTCGTGCTCGGGCAtt                                                                            <  7:151292/149‑1 (MQ=255)
                                                                       tCACGTGCCCACCATGCCGGGTCGCGTTCGAAGGTGATGCTGCGGCCGTTGTCG‑ATG‑‑CGCCCAATGCGGT‑ATG‑GCCCGCTGCCGACTGGCTTGTCGAAGCCGGCACCGTTG‑GCAAAGTCGCGCTGCTGCAGGTCGTGCTCGGGCATTAc                                                                          <  5:263969/149‑1 (MQ=255)
                                                                         aCGTGCCCACCATGCCGGGTCGCGTTCGAAGGTGATGCTGCGGCCGTTGTCG‑ATG‑‑CGCCCAATGCGGT‑ATG‑GCCCGCTGCCGACTGGCTTGTCGAAGCCGGCACCGTTG‑GCAAAGTCGCGCTGCTGCAGGTCGTGCTCGGGCATTAcc                                                                         <  2:188730/148‑1 (MQ=255)
                                                                           gTGCCCACCATGCCGGGTCGCGTTCGAAGGTGATGCTGCGGCCGTTGTCG‑ATG‑‑CGCCCAATGCGGT‑ATG‑GCCCGCTGCCGACTGGCTTGTCGAAGCCGGCACCGTTG‑GCAAAGTCGCGCTGCTGCAGGTCGTGCTCGGGCATTACCgg                                                                       >  6:118057/1‑148 (MQ=255)
                                                                             gCCCACCATGCCGGGTCGCGTTCGAAGGTGATGCTGCGGCCGTTGTCG‑ATG‑‑CGCCCAATGCGGT‑ATG‑GCCCGCTGCCGACTGGCTTGTCGAAGCCGGCACCGTTG‑GCAAAGTCGCGCTGCTGCAGGTCGTGCTCGGGCATTACCGGCAg                                                                    >  6:137618/1‑149 (MQ=255)
                                                                                  ccaTGCCGGGTCGCGTTCGAAGGTGATGCTGCGGCCGTTGTCG‑ATG‑‑CGCCCAATGCGGT‑ATG‑GCCCGCTGCCGACTGGCTTGTCGAAGCCGGCACCGTTG‑GCAAAGTCGCGCTGCTGCAGGTCGTGCTCGGGCATTACCGGCAGGCTgg                                                               <  5:137618/149‑1 (MQ=255)
                                                                                   cctGCCGGGTCGCGTTCGAAGGTGAGGCTGCGGCCGTGGTCG‑ATG‑‑CGCCCAATGCGGT‑ATG‑GCCCGCTGCCGACAGGCTTGTCGAAGCCGGCACCGTTG‑GCAAAGTCGCGCTGCTGCAGGGCTTGCTCGGGCATTACCGGCAGGCTGGc                                                              <  6:183265/147‑1 (MQ=255)
                                                                                       ccGGGTCGCGTTCGAAGGTGATGCTGCGGCCGTTGTCG‑ATG‑‑CGCCCAATGCGGT‑ATG‑GCCCGCTGCCGACTGGCTTGTCGAAGCCGGCACCGTTG‑GCAAAGTCGCGCTGCTGCAGGTCGTGCTCGGGCATTACCGGCAGGCTGGCGAg                                                           <  5:242990/148‑1 (MQ=255)
                                                                                         gggTCGCGTTCGAAGGTGATGCTGCGGCCGTTGTCG‑ATG‑‑CGCCCAATGCGGT‑ATG‑ACCCGCTGCCGACTGGCTTGTCGAAGCCGGCACCGTTG‑GCAAAGTCGCGCTGCTGCAGGTCGTGCTCGGGCATTACCGGCAGGCTGGCGAGGTc                                                        <  8:304075/149‑1 (MQ=255)
                                                                                         gggTCGCGTTCGAAGGTGATGCTGCGGCCGTTGTCG‑ATG‑‑CGCCCAATGCGGT‑ATG‑ACCCGCTGCCGACTGGCTTGTCGAAGCCGGCACCGTTG‑GCAAAGTCGCGCTGCTGCAGGTCGTGCTCGGGCATTACCGGCAGGCTGGCGAGGTc                                                        <  8:198032/149‑1 (MQ=255)
                                                                                              gcgTTCGAAGGTGATGCTGCGGCCGTTGTCG‑ATG‑‑CGCCCAATGCGGT‑ATG‑GCCCGCTGCCGACTGGCTTGTCGAAGCCGGCACCGTTG‑GCAAAGTCGCGCTGCTGCAGGTCGTGCTCGGGCATTACCGGCAGGCTGGCGAGGTCCAgcg                                                   >  8:264019/1‑149 (MQ=255)
                                                                                                gTTCGAAGGTGATGCTGCGGCCGTTGTCG‑ATG‑‑CGCCCAATGCGGT‑ATG‑GCCCGCTGCCGACTGGCTTGTCGAAGCCGGCACCGTTG‑GCAAAGTCGCGCTGCTGCAGGTCGTGCTCGGGCATTACCGGCAGGCTGGCGAGGTCCAgcgc                                                  >  4:49124/1‑148 (MQ=255)
                                                                                                   cGAAGGTGATGCTGCGGCCGTTGTCG‑ATG‑‑CGCCCAATGCGGT‑ATG‑GTCCGCTGCCGACTGGCTTGTCGAAGCCGGCACCGTTG‑GCAAAGTCGCGCTGCTGCAGGTCGTGCTCGGGCATTACCGGCAGGCTGGCGAGGTCCAGCGCCAg                                               <  5:178865/148‑1 (MQ=255)
                                                                                                           aTGCTGCGGCCGTTGTCG‑ATG‑‑CGCCGAATGCGGT‑ATG‑GCCCGCTGCCGACTGGCTTGTCGAAGCCGGCAGCGTTG‑GCAAAGTCGCGCTGCTGCAGGTCGTGCTCGGGCATTACCGGCAGGCTGGCGAGGGCCAGCGCCAGGGTGCGGcc                                      <  2:127986/149‑1 (MQ=255)
                                                                                                            tgctgcGGCCGTTGTCG‑ATG‑‑CGCCCAATGCGGT‑ATG‑GCCCGCTGCCGACTGGCTTGTCGAAGCCGGCACCGTTG‑GCAAAGTCGCGCTGCTGCAGGTCGTGCTCGGGCATTACCGGCAGGCTGGCGAGGTCCAGCGCCAGGGTGCGGCCg                                     <  2:73247/149‑1 (MQ=255)
                                                                                                             gctgcGGCCGTTGTCG‑ATG‑‑CGCCCAATGCGGT‑ATG‑GCCCGCTGCCGACTGGCTTGTCGAAGCCGGCACCGTTG‑GCAAAGTCGCGCTGCTGCAGGTCGTGCTCGGGCATTACCGGCAGGCTGGCGAGGTCCAGCGCCAGGGTGCGGCCGt                                    >  3:134895/1‑149 (MQ=255)
                                                                                                                        tGTCG‑ATG‑‑CGCCCAATGCGGT‑ATG‑GCCCGCTGCCGACTGGCTTGTCGAAGCCGGCACCGTTG‑GCAAAGTCGCGCTGCTGCAGGTCGTGCTCGGGCATTACCGGCAGGCTGGCGAGGTCCAGCGCCAGGGTGCGGCCGTGCTCGGGCtt                          >  1:175065/1‑148 (MQ=255)
                                                                                                                          tCG‑ATG‑‑CGCCCAATGCGGT‑ATG‑GCCCGCTGCCGACTGGCTTGTCGAAGCCGGCACCGTTG‑GCAAAGTCGCGCTGCTGCAGGTCGTGCTCGGGCATTACCGGCAGGCTGGCGAGGTCCAGCGCCAGGGTGCGGCCGTGCTCGGGCTTCaa                       <  3:49124/149‑1 (MQ=255)
                                                                                                                            g‑ATG‑‑CGCCCAATGCGGT‑ATG‑GCCCGCTGCCGACTGGCTTGTCGAAGCCGGCACCGTTG‑GCAAAGTCGCGCTGCTGCAGGTCGTGCTCGGGCATTACCGGCAGGCTGGCGAGGTCCAGCGCCAGGGTGCGGCCGTGCTCGGGCTTCAAGt                     <  4:134895/149‑1 (MQ=255)
                                                                                                                                       cAATGCGGT‑ATG‑GCCCGCTGCCGACTGGCTTGTCGAAGCCGGCACCGTTG‑GCAAAGTCGCGCTGCTGCAGGTCGTGCTCGGGCATTACCGGCAGGCTGGCGAGGTCCAGCGCCAGGGTGCGGCCGTGCTCGGGCTTCAAGTCGAATCg              >  6:66532/1‑148 (MQ=255)
                                                                                                                                        aaTGCGGT‑ATG‑GCCCGCTGCCGACTGGCTTGTCGAAGCCGGCACCGTTG‑GCAAAGTCGCGCTGCTGCAGGTCGTGCTCGGGCATTACCGGCAGGCTGGCGAGGTCCAGCGCCAGGGTGCGGCCGTGCTCGGGCTTCAAGTCGAATCGCa            >  1:21186/1‑149 (MQ=255)
                                                                                                                                        aaTGCGGT‑ATG‑GCCCGCTGCCGACTGGCTTGTCGAAGCCGGCACCGTTG‑GCAAAGTCGCGCTGCTGCAGGTCGTGCTCGGGCATTACCGGCAGGCTGGCGAGGTCCAGCGCCAGGGTGCGGCCGTGCTCGGGCTTCAAGTCGAATCGCa            >  4:79802/1‑149 (MQ=255)
                                                                                                                                                     gCCCGCTGCCGACTGGCTTGTCGAAGCCGGCACCGTTG‑GCAAAGTCGCGCTGCTGCAGGTCGTGCTCGGGCATTACCGGCAGGCTGGCGAGGTCCAGCGCCAGGGTGCGGCCGTGCTCGGGCTTCAAGTCGAATCGCACGCTGTGCAg  <  7:264019/148‑1 (MQ=255)
                                                                                                                                                     gCCCGCTGCCGACTGGCTTGTCGAAGCCGGCACCGTTG‑GCAAAGTCGCGCTGCTGCAGGTCGTGCTCGGGCATTACCGGCAGGCTGGCGAGGTCCAGCGCCAGGGTGCGGCCGTGCTCGGGCTTCAAGTCGAATCGCACGCTGTGCAg  <  4:48786/148‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
CCACTTCGGTGTCGCCGAAGTACTCGATGCGCAGTTTGTCGAAATTGTAGCGGCCACGGCTGGCGGGCAGGTCACGTGCCCACCATGCCGGGTCGCGTTCGAAGGTGATGCTGCGGCCGTTGTCG‑ATG‑‑CGCCCAATGCGGT‑ATG‑GCCCGCTGCCGACTGGCTTGTCGAAGCCGGCACCGTTG‑GCAAAGTCGCGCTGCTGCAGGTCGTGCTCGGGCATTACCGGCAGGCTGGCGAGGTCCAGCGCCAGGGTGCGGCCGTGCTCGGGCTTCAAGTCGAATCGCACGCTGTGCAG  >  NC_002947/3463475‑3463766

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑