Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,491,487 T→C 6.9% E311G (GAG→GGG)  PP_3096 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,491,4870TC 6.9% 209.8 / 4.3 72E311G (GAG→GGG) PP_3096hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (30/37);  new base C (2/3);  total (32/40)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.78e-01

GGTTCACAAGGCGACCCCATCGTAAGGAATGTGGAAGACGGCGCAACGCGAAGGCTCCGCGTCACGACCAGGGCGTTTGAGCCAACCCAGCCAGCCCAGGGCGACATTGCCGCTGCGGCCCAGTTGGGCGCGTGGTACCGCCTCGCGCTTGAGGCGCGGGGCAATTTCGAAATCCAGTTCGGCACCTATATAGAAGCGCACCAACTCGACCAGCTTGCGGTAGCAGGCGGTGCCCGGCTGGAAGCGCTGGTAGTCGGCCAGGCTGAGCGGGCCCAGCTCGATGCGGA  >  NC_002947/3491345‑3491631
                                                                                                                                              |                                                                                                                                                
ggTTCACAAGGCGACCCCATCGTAAGGAATGTGGAAGACGGCGCAACGCGAAGGCTCCGCGTCACGACCAGGGCGTTTGAGCCAACCCAGCCAGCCCAGGGCGACATTGCCGCTGCGGCCCAGTTGGGCGCGTGGTACCGCCTCGCGCt                                                                                                                                            >  7:117632/1‑149 (MQ=255)
 gTTCACAAGGCGACCCCATCGTAAGGAATGTGGAAGACGGCGCAACGCGAAGGCTCCGCGTCACGACCAGGGCGTTTGAGCCAACCCAGCCAGCCCAGGGCGACATTGCCGCTGCGGCCCAGTTGGGCGCGTGGTACCGCCTCGCGCtt                                                                                                                                           <  4:149135/149‑1 (MQ=255)
   tCACAAGGCGACCCCATCGTAAGGAATGTGGAAGACGGCGCAACGCGAAGGCTCCGCGTCACGACCAGGGCGTTTGAGCCAACCCAGCCAGCCCAGGGCGACATTGCCGCTGCGGCCCAGTTGGGCGCGTGGTACCGCCTCGCGCtt                                                                                                                                           >  1:109783/1‑147 (MQ=255)
     acaAGGCGACCCCATCGTAAGGAATGTGGAAGACGGCGCAACGCGAAGGCTCCGCGTCACGACCAGGGCGTTTGAGCCATCCCAGCCAGCCCAGGGCGACATTGCCGCTGCGGCCCAGTTGGGCGCGTGGTACCGCCTCGCGCTTGAgg                                                                                                                                       >  1:239184/1‑149 (MQ=255)
      caAGGCGACCCCATCGTAAGGAATGTGGAAGACGGCGCAACGCGAAGGCTCCGCGTCACGACCAGGGCGTTTGAGCCAACCCAGCCAGCCCAGGGCGACATTGCCGCTGCGGCCCAGTTGGGCGCGTGGTACCGCCTCGCGCTTGAGgc                                                                                                                                      <  8:32248/149‑1 (MQ=255)
       aaGGCGACCCCATCGTAAGGAATGTGGAAGACGGCGCAACGCGAAGGCTCCGCGTCACGACCAGGGCGTTTGAGCCAACCCAGCCAGCCCAGGGCGACATTGCCGCTGCGGCCCAGTTGGGCGCGTGGTACCGCCTCGCGCTTGAGgcg                                                                                                                                     >  7:29421/1‑149 (MQ=255)
          gCGACCCCATCGTAAGGAATGTGGAAGACGGCGCAACGCGAAGGCTCCGCGTCACGACCAGGGCGTTTGAGCCAACCCAGCCAGCCCAGGGCGACATTGCCGCTGCGGCCCAGTTGGGCGCGTGGTACCGCCTCGCGCTTGAGGCGCgg                                                                                                                                  >  5:90215/1‑149 (MQ=255)
           cGACCCCATCGTAAGGAATGTGGAAGACGGCGCAACGCGAAGGCTCCGCGTCACGACCAGGGCGTTTGAGCCATCCCAGCCAGCCCAGGGCGACATTGCCGCTGCGGCCCAGTTGGGCGCGTGGTACCGCCTCGCGCTTGAGGCGCggg                                                                                                                                 <  2:239184/149‑1 (MQ=255)
           cGACCCCATCGTAAGGAATGTGGAAGACGGCGCAACGCGAAGGCTCCGCGTCACGACCAGGGCGTTTGAGCCAACCCAGCCAGCCCAGGGCGACATTGCCGCTGCGGCCCAGTTGGGCGCGTGGTACCGCCTCGCGCTTGAGGCGCggg                                                                                                                                 <  1:17899/149‑1 (MQ=255)
            gACCCCATCGTAAGGAATGTGGAAGACGGCGCAACGCGAAGGCTCCGCGTCACGACCAGGGCGTTTGAGCCAACCCAGCCAGCCCAGGGCGACATTGCCGCTGCGGCCCAGTTGGGCGCGTGGTACCGCCTCGCGCTTGAGGCGCgggg                                                                                                                                >  3:316001/1‑149 (MQ=255)
             aCCCCATCGTAAGGAATGTGGAAGACGGCGCAACGCGAAGGCTCCGCGTCACGACCAGGGCGTTTGAGCCAACCCAGCCAGCCCAGGGCGACATTGCCGCTGCGGCCCAGTTGGGCGCGTGGTACCGCCTCGCGCTTGAGGCGCGGGGc                                                                                                                               <  1:16415/149‑1 (MQ=255)
               cccATCGTAAGGAATGTGGAAGACGGCGCAACGCGAAGGCTCCGCGTCACGACCAGGGCGTTTGAGCCAACCCAGCCAGCCCAGGGCGACATTGCCGCTGCGGCCCAGTTGGGCGCGTGGTACCGCCTCGCGCTTGAGGCGCGGGGCaa                                                                                                                             >  8:64694/1‑149 (MQ=255)
               cccATCGTAAGGAATGTGGAAGACGGCGCAACGCGAAGGCTCCGCGTCACGACCAGGGCGTTTGAGCCAACCCAGCCAGCCCAGGGCGACATTGCCGCTGCGGCCCAGTTGGGCGCGTGGTACCGCCCCGCGCCTCAAGCGCGAGGcgg                                                                                                                             <  7:40302/149‑3 (MQ=255)
                 cATCGTAAGGAATGTGGAAGACGGCGCAACGCGAAGGCTCCGCGTCACGACCAGGGCGTTTGAGCCAACCCAGCCAGCCCAGGGCGACATTGCCGCTGCGGCCCAGTTGGGCGCGTGGTACCGCCTCGCGCTTGAGGCGCGGGGCAAtt                                                                                                                           <  8:117632/149‑1 (MQ=255)
                 cATCGTAAGGAATGTGGAAGACGGCGCAACGCGAAGGCTCCGCGTCACGACCAGGGCGTTTGAGCCAACCCAGCCAGCCCAGGGCGACATTGCCGCTGCGGCCCAGTTGGGCGCGTGGTACCGCCTCGCGCTTGAGGCGCGGGGCAAtt                                                                                                                           >  1:6389/1‑149 (MQ=255)
                   tCGTAAGGAATGTGGAAGACGGCGCAACGCGAAGGCTCCGCGTCACGACCAGGGCGTTTGAGCCAACCCAGCCAGCCCAGGGCGACATTGCCGCTGCGGCCCAGTTGGGCGCGTGGTACCGCCTCGCGCTTGAGGCGCGGGGCAATTTc                                                                                                                         <  6:133963/149‑1 (MQ=255)
                   tCGTAAGGAATGTGGAAGACGGCGCAACGCGAAGGCTCCGCGTCACGACCAGGGCGTTTGAGCCAACCCAGCCAGCCCAGGGCGACATTGCCGCTGCGGCCCAGTTGGGCGCGTGGTACCGCCTCGCGCTTGAGGCGCGGGGCAATTTc                                                                                                                         >  1:112199/1‑149 (MQ=255)
                     gTAAGGAATGTGGAAGACGGCGCAACGCGAAGGCTCCGCGTCACGACCAGGGCGTTTGAGCCAACCCAGCCAGCCCAGGGCGACATTGCCGCTGCGGCCCAGTTGGGCGCGTGGTACCGCCTCGCGCTTGAGGCGCGGGGCAATTTCg                                                                                                                        <  3:50178/148‑1 (MQ=255)
                      tAAGGAATGTGGAAGACGGCGCAACGCGAAGGCTCCGCGTCACGACCAGGGCGTTTGAGCCAACCCAGCCAGCCCAGGGCGACATTGCCGCTGCGGCCCAGTTGGGCGCGTGGTACCGCCTCGCGCTTGAGGCGCGGGGCAATTTCGaa                                                                                                                      <  7:67668/149‑1 (MQ=255)
                      tAAGGAATGTGGAAGACGGCGCAACGCGAAGGCTCCGCGTCACGACCAGGGCGTTTGAGCCAACCCAGCCAGCCCAGGGCGACATTGCCGCTGCGGCCCAGTTGGGCGCGTGGTACCGCCTCGCGCTTGAGGCGCGGGGCAATTTCGa                                                                                                                       >  6:106414/1‑148 (MQ=255)
                           aaTGTGGAAGACGGCGCAACGCGAAGGCTCCGCGTCACGACCAGGGCGTTTGAGCCAACCCAGCCAGCCCAGGGCGACATTGCCGCTGCGGCCCAGTTGGGCGCGTGGTACCGCCTCGCGCTTGAGGCGCGGGGCAATTTCGAAATCCa                                                                                                                 <  5:136924/149‑1 (MQ=255)
                            aTGTGGAAGACGGCGCAACGCGAAGGCTCCGCGTCACGACCAGGGCGTTTGAGCCAACCCAGCCAGCCCAGGGCGACATTGCCGCTGCGGCCCAGTTGGGCGCGTGGTACCGCCTCGCGCTTGAGGCGCGGGGCAATTTCGAAATCCAg                                                                                                                <  7:128118/149‑1 (MQ=255)
                            aTGTGGAAGACGGCGCAACGCGAAGGCTCCGCGTCACGACCAGGGCGTTTGAGCCAACCCAGCCAGCCCAGGGCGACATTGCCGCTGCGGCCCAGTTGGGCGCGTGGTACCGCCTCGCGCTTGAGGCGCGGGGCAATTTCGAAATCCAg                                                                                                                <  6:109445/149‑1 (MQ=255)
                                ggAAGACGGCGCAACGCGAAGGCTCCGCGTCACGACCAGGGCGTTTGAGCCAACCCAGCCAGCCCAGGGCGACATTGCCGCTGCGGCCCAGTTGGGCGCGTGGTACCGCCTCGCGCTTGAGGCGCGGGGCAATTTCGAAATCCAGTTCg                                                                                                            >  4:326638/1‑149 (MQ=255)
                                 gAAGACGGCGCAACGCGAAGGCTCCGCGTCACGACCAGGGCGTTTGAGCCAACCCAGCCAGCCCAGGGCGACATTGCCGCTGCGGCCCAGTTGGGCGCGTGGTACCGCCTCGCGCTTGAGGCGCGGGGCAATTTCGAAATCCAGTTCg                                                                                                            <  7:64201/148‑1 (MQ=255)
                                  aaGACGGCGCAACGCGAAGGCTCCGCGTCACGACCAGGGCGTTTGAGCCAACCCAGCCAGCCCAGGGCGACATTGCCGCTGCGGCCCAGTTGGGCGCGTGGTACCGCCTCGCGCTTGAGGCGCGGGGCAATTTCGAAATCCAGTTCGGc                                                                                                          <  2:91679/149‑1 (MQ=255)
                                      cGGCGCAACGCGAAGGCTCCGCGTCACGACCAGGGCGTTTGAGCCAACCCAGCCAGCCCAGGGCGACATTGCCGCTGCGGCCCAGTTGGGCGCGTGGTACCGCCTCGCGCTTGAGGCGCGGGGCAATTTCGAAATCCAGTTCGGCACCt                                                                                                      <  2:6389/149‑1 (MQ=255)
                                      cGGCGCAACGCGAAGGCTCCGCGTCACGACCAGGGCGTTTGAGCCAACCCAGCCAGCCCAGGGCGACATTGCCGCTGCGGCCCAGTTGGGCGCGTGGTACCGCCTCGCGCTTGAGGCGCGGGGCAATTTCGAAATCCAGTTCGGCACCt                                                                                                      <  7:38768/149‑1 (MQ=255)
                                       ggCGCAACGCGAAGGCTCCGCGTCACGACCAGGGCGTTTGAGCCAACCCAGCCAGCCCAGGGCGACATTGCCGCTGCGGCCCAGTTGGGCGCGTGGTACCGCCTCGCGCTTGAGGCGCGGGGCAATTTCGAAATCCAGTTCGGCACCta                                                                                                     >  6:249620/1‑149 (MQ=255)
                                         cgcAACGCGAAGGCTCCGCGTCACGACCAGGGCGTTTGAGCCAACCCAGCCAGCCCAGGGCGACATTGCCGCTGCGGCCCAGTTGGGCGCGTGGTACCGCCTCGCGCTTGAGGCGCGGGGCAATTTCGAAATCCAGTTCGGCACCtata                                                                                                   <  2:109783/149‑1 (MQ=255)
                                          gcAACGCGAAGGCTCCGCGTCACGACCAGGGCGTTTGAGCCAACCCAGCCAGCCCAGGGCGACATTGCCGCTGCGGCCCAGTTGGGCGCGTGGTACCGCCTCGCGCTTGAGGCGCGGGGCAATTTCGAAATCCAGTTCGGCACCtata                                                                                                   >  1:276743/1‑148 (MQ=255)
                                          gcAACGCGAAGGCTCCGCGTCACGACCAGGGCGTTTGAGCCAACCCAGCCAGCCCAGGGCGACATTGCCGCTGCGGCCCAGTTGGGCGCGTGGTACCGCCTCGCGCTGGAGGCGCGGGGCAATTTCGAAATCCAGTTCGGCACCtata                                                                                                   >  4:89006/1‑148 (MQ=255)
                                              cgcgAAGGCTCCGCGTCACGACCAGGGCGTTTGAGCCAACCCAGCCAGCCCAGGGCGACATTGCCGCTGCGGCCCAGTTGGGCGCGTGGTACCGCCTCGCGCTTGAGGCGCGGGGCAATTTCGAAATCCAGTTCGGCACCTATATAGaa                                                                                              <  8:29421/149‑1 (MQ=255)
                                                cgAAGGCTCCGCGTCACGACCAGGGCGTTTGAGCCAACCCAGCCAGCCCAGGGCGACATTGCCGCTGCGGCCCAGTTGGGCGCGTGGTACCGCCTCGCGCTTGAGGCGCGGGGCAATTTCGAAATCCAGTTCGGCACCTATATAGAAgc                                                                                            <  7:83614/149‑1 (MQ=255)
                                                cgAAGGCTCCGCGTCACGACCAGGGCGTTTGAGCCAACCCAGCCAGCCCAGGGCGACATTGCCGCTGCGGCCCAGTTGGGCGCGTGGTACCGCCTCGCGCTTGAGGCGCGGGGCAATTTCGAAATCCAGTTCGGCACCTATATAGAAgc                                                                                            <  7:24131/149‑1 (MQ=255)
                                                       tCCGCGTCACGACCAGGGCGTTTGAGCCAACCCAGCCAGCCCAGGGCGACATTGCCGCTGCGGCCCAGTTGGGCGCGTGGTACCGCCTCGCGCTTGAGGCGCGGGGCAATTTCGAAATCCAGTTCGGCACCTATATAGAAGCGCACCaa                                                                                     >  5:333373/1‑149 (MQ=255)
                                                            gTCACGACCAGGGCGTTTGAGCCAACCCAGCCAGCCCAGGGCGACATTGCCGCTGCGGCCCAGTTGGGCGCGTGGTACCGCCTCGCGCTTGAGGCGCGGGGCAATTTCGAAATCCAGTTCGGCACCTATATAGAAGCGCACCAACTCGa                                                                                <  3:326638/149‑1 (MQ=255)
                                                             tCACGACCAGGGCGTTTGAGCCAACCCAGCCAGCCCAGGGCGACATTGCCGCTGCGGCCCAGTTGGGCGCGTGGTACCGCCTCGCGCTTGAGGCGCGGGGCAATTTCGAAATCCAGTTCGGCACCTATATAGCAGCGCACCAACTCGAc                                                                               <  4:316001/149‑1 (MQ=255)
                                                             tCACGACCAGGGCGTTTGAGCCAACCCAGCCAGCCCAGGGCGACATTGCCGCTGCGGCCCAGTTAGGCGCGTGGTACCGCCTCGCGCTTGAGGCGCGGGGCAATTTCGAAATCCAGTTCGGCACCTATATAGAAGCGCACCAACTCGAc                                                                               >  7:204935/1‑149 (MQ=255)
                                                              cACGACCAGGGCGTTTGAGCCAACCCAGCCAGCCCAGGGCGACATTGCCGCTGCGGCCCAGTTGGGCGCGTGGTACCGCCTCGCGCTTGAGGCGCGGGGCAATTTCGAAATCCAGTTCGGCACCTATATAGAAGCGCACCAACTCGAc                                                                               >  2:340404/1‑148 (MQ=255)
                                                                cGACCAGGGAGTTTGAGCCAACCCAGCCAGCCCAGCGCGACATTGCCGCTGCGGCCCAGTTGGGCGCGTGGTACCGCCTCGCGCTTGAGGCGCGGGGCAAATTCGAAAACCAGTTCGGCACCTATATAGAAGCGCACCAACTCCAccc                                                                             >  6:76227/1‑147 (MQ=255)
                                                                 gACCAGGGCGTTTGAGCCAACCCAGCCAGCCCAGGGCGACATTGCCGCTGCGGCCCAGTAGGGCGCGTGGTACCGCCTCGCGCTAGAGGCGCGCGGCAAATTCGAAATCCAGTAAGGCACCTATATAGAAGCCCACCAACTCGACCAGc                                                                           >  2:117505/1‑149 (MQ=255)
                                                                  aCCAGGGCGTTTGAGCCAACCCAGCCAGCCCAGGGCGACATTGCCGCTGCGGCCCAGTTGGGCGCGTGGTACCGCCTcgc                                                                                                                                               >  6:308681/1‑80 (MQ=255)
                                                                   ccAGGGCGTTTGAGCCAACCCAGCCAGCCCAGGGCGACATTGCCGCTGCGGCCCAGTTGGGCGCGTGGTACCGCCTCGCGCTTGAGGcgcg                                                                                                                                   <  1:314141/91‑1 (MQ=255)
                                                                   ccAGGGCGTTTGAGCCAACCCAGCCAGCCCAGGGCGACATTGCCGCTGCGGCCCAGTTGGGCGCGTGGTACCGCCTCGCGCTTGAGGcgcg                                                                                                                                   >  2:314141/1‑91 (MQ=255)
                                                                    cAGGGCGTTTGAGCTATCCCAGCCAGCCCAGGGCGACATTGCCGCTGCGGCCCAGTTGGGCGCGTGGTACCGCCTCGCGCTTGAGGCGCGGGGCAATTTCGAAATCCAGTTCGGCACCTATATAGAAGCGCACCAACTCGACCAGCTTg                                                                        <  1:117505/149‑1 (MQ=255)
                                                                       ggCGTTTGAGCCAACCCAGCCAGCCCAGGGCGACATTGCCGCTGCGGCCCAGTTGGGCGCGTGGTACCGCCTCGCGCTTGAGGCGCGGGGCAATTTCGAAATCCAGTTCGGCACCTATATAGAAGCGCACCAACTCGACCAGCTTGCgg                                                                     <  6:333373/149‑1 (MQ=255)
                                                                       ggCGTTTGAGCCAACCCAGCCAGCCCAGGGCGACATTGCCGCTGCGGCCCAGTTGGGCGCGTGGTACCGCCTCGCGCTTGAGGCGCGGGGCAATTTCGAAATCCAGTTCGGCACCTATATAGAAGCGCACCAACTCGACCAGCTTGCgg                                                                     <  5:106414/149‑1 (MQ=255)
                                                                         cGTTTGAGCCAACCCAGCCAGCCCAGGGCGACATTGCCGCTGCGGCCCAGTTGGGCGCGTGGTACCGCCTCGCGCTTGAGGCGCGGGGCAATTTCGAAATCCAGTTCGGCACCTATATAGAAGCGCACCAACTCGACCAGCTTGCGGTa                                                                   <  2:112199/149‑1 (MQ=255)
                                                                                gCCAACCCAGCCAGCCCAGGGCGACATTGCCGCTGCGGCCCAGTTGGGCGCGTGGTACCGCCTCGCGCTTGAGGCGCGGGGCAATTTCGAAATCCAGTTCGGCACCTATATAGAAGCGCACCAACTCGACCAGCTTGCGGTAGCAGGCg                                                            >  5:200409/1‑149 (MQ=255)
                                                                                 ccAACCCAGCCAGCCCAGGGCGACATTGCCGCTGCGGCCCAGTTGGGCGCGTGGTACCGCCTCGCGCTTGAGGCGCGGGGCAATTTCGAAATCCAGTTCGGCACATATATAGAAGCGCACAAACTCGACCAGCTTGCGGTAGCAGGCgt                                                           >  6:324293/1‑148 (MQ=255)
                                                                                  cAACCCAGCCAGCCCAGGGCGACATTGCCGCTGCGGCCCAGTTGGGCGCGTGGTACCGCCTCGCGCTTGAGGCGCGGGGCAATTTCGAAATCCAGTTCGGCACCTATATAGAAGCGCACCAACTCGACCAGCTTGCGGTAGCAGGCGGt                                                          <  2:81732/149‑1 (MQ=255)
                                                                                  cAACCCAGCCAGCCCAGGGCGACATTGCCGCTGCGGCCCAGTTGGGCGCGTGGTACCGCCTAGCCCTTCAGCCCCGGGGCAAATTCGAAATACAGATCGGCACCTATATAGAAGCGCACCAACTCGACCAGCTAGCGGTAGCAGCCGGt                                                          >  8:58383/1‑149 (MQ=255)
                                                                                    aCCCAGCCAGCCCAGGGCGACATTGCCGCTGCGGCCCAGTTGGGCGCGTGGTACCGCCTCGCGCTTGAGGCGCGGGGCAATTTCGAAATCCAGTTCGGCACCTATATAGAAGCGCACCAACTCGACCAGCTTGCGGTAGCAGGCGGTGc                                                        >  5:273640/1‑149 (MQ=255)
                                                                                      ccagccagCCCAGGGCGACATTGCCGCTGCGGCCCAGTTGGGCGCGTGGTACCGCCTCGCGCTTGAGGCGCGGGGCAATTTCGAAATCCAGTTCGGCACCTATATAGAAGCGCACCAACTCGACCAGCTTGCGGTAGCAGGCGGTGccc                                                      >  7:136696/1‑149 (MQ=255)
                                                                                            agccCAGGGCGACATTGCCGCTGCGGCCCAGTTGGGCGCGTGGTACCGCCTCGCGCTTGAGGCGCGGGGCAATTTCGAAATCCAGTTCGGCACCTATATAGAAGCGCACCAACTCGACCAGCTTGCGGTAGCAGGCGGTGCCCGGATg                                                 >  3:284268/1‑148 (MQ=255)
                                                                                              cccAGGGCGACATTGCCGCTGCGGCCCAGTTGGGCGCGTGGTACCGCCTCGCGCTTGAGGCGCGGGGCAATTTCGAAATCCAGTTCGGCACCTATATAGAAGCGCACCAACTCGACCAGCTTGCGGTAGCAGGCGGTGCCCGGCTGGaa                                              <  8:136696/149‑1 (MQ=255)
                                                                                                     cGACATTGCCGCTGCGGCCCAGTTGGGCGCGTGGTACCGCCTCGCGCTTGAGGCGCGGGGCAATTTCGAAATCCAGTTCGGCACCTATATAGAAGCGCACCAACTCGACCAGCTTGCGGTAGCAGGCGGTGCCCGGCTGGAAGCGCTgg                                       <  6:90215/149‑1 (MQ=255)
                                                                                                     cGACATTGCCGCTGCGGCCCAGTTGGGCGCGTGGTACCGCCTCGCGCTTGAGGCGCGGGGCAATTTCGAAATCCAGTTCGGCACCTATATAGAAGCGCACCAACTCGACCAGCTTGCGGTAGCAGGCGGTGCCCGGCTGGAAGCGCTgg                                       <  7:64694/149‑1 (MQ=255)
                                                                                                             ccGCTGCGGCCCAGGTGGGCGCGTGGTACCGCCTCGCGCTTGAGGCGCGGGGCAATTTCGAAATCCAGTTCGGCACCTATATAGAAGCGCACCAACTCGACCAGCTTGCGGTAGCAGGCGGTGCCCGGCTGGAAGCGCTGGTAGTCGGc                               <  8:216303/149‑1 (MQ=255)
                                                                                                               gCTGCGGCCCAGTTGGGCGCGTGGTACCGCCTCGCGCTTGAGGCGCGGGGCAATTTCGAAATCCAGTTCGGCACCTATATAGAAGCGCACCAACTCGACCAGCTTGCGGTTGCAGTCTGTGCCAGGCTTGAAGCGCTGGTAGTCGGCCa                             <  5:76227/149‑1 (MQ=255)
                                                                                                                   cGGCCCAGTTGGGCGCGTGGTACCGCCTCGCGCTTGAGGCGCGGGGCAATTTCGAAATCCAGTTCGGCACCTATATAGAAGCGCACCAACTCGACCAGCTTGCGGTAGCAGGCGGTGCCCGGCTGGAAGCGCTGGTAGTCGGCCAGGc                          <  1:4909/148‑1 (MQ=255)
                                                                                                                          gTTGGGCGCGTGGTACCGCCTCGCGCTTGAGGCGCGGGGCAATTTCGAAATCCAGTTCGGCACCTATATAGAAGCGCACCAACTCGACCAGc                                                                           >  2:86768/1‑92 (MQ=255)
                                                                                                                          gTTGGGCGCGTGGTACCGCCTCGCGCTTGAGGCGCGGGGCAATTTCGAAATCCAGTTCGGCACCTATATAGAAGCGCACCAACTCGACCAGc                                                                           <  1:86768/92‑1 (MQ=255)
                                                                                                                                  cgTGGTACCGCCTCGCGCTTGAGGCGCGGGGCAATTTCGAAATCCAGTTCGGCACCTATATAGAAGCGCACCAACTCGACCAGCTTGCGGTAGCAGGCGGTGCCCGGCTg                                                 <  5:189588/110‑1 (MQ=255)
                                                                                                                                  cgTGGTACCGCCTCGCGCTTGAGGCGCGGGGCAATTTCGAAATCCAGTTCGGCACCTATATAGAAGCGCACCAACTCGACCAGCTTGCGGTAGCAGGCGGTGCCCGGCTg                                                 >  6:189588/1‑110 (MQ=255)
                                                                                                                                     gaaattgCCCCGCGCCTCAAGCGCGGGGCAATTTCGAAATCCAGTTCGGCACCTATATAGAAGCGCACCAACTCGACCAGCTTGCGGTAGCAGGCGGTGCCCGGTTGGAAGCGCTGGTAGTCGGCCAGGCTGAGCGGGCCCAGCTCGAt       >  4:245168/7‑149 (MQ=255)
                                                                                                                                     gaaattgCCCCGCGCCTCAAGCGCGGGGCAATTTCGAAATCCAGTTCGGCACCTATATAGAAGCGCACCAACTCGACCAGCTTGCGGTAGCAGGCGGTGCCCGGTTGGAAGCGCTGGTAGTCGGCCAGGCTGAGCGGGCCCAGCTCGAt       >  2:55792/7‑149 (MQ=255)
                                                                                                                                       tACCGCCTCGCGCTTGAGGCGCGGGGCAATTTCGAAATCCAGTTCGGCACCTATATAGAAGCGCACCAACTCGACCAGCTTGCGGTAGCAGGCGGTGCCCGGCTGGAAGCGCTGGTAGTCGGCCAGGCTGAGCGGGCCCAGCTCGATGc     <  1:340404/149‑1 (MQ=255)
                                                                                                                                       tACCGCCTCGCGCTTGAGGCGCGGGGCAATTTCGAAATCCAGTTCGGCACCTATATAGAAGCGCACCAACTCGACCAGCTTGCGGTAGCAGGCGGTGCCCGGCTGGAAGCGCTGGTAGTCGGCCAGGCTGAGCGGGCCCAGCTCGATGc     >  6:309484/1‑149 (MQ=255)
                                                                                                                                           gCCCCGCGCCTCAAGCGCGGGGCAATTTCGAAATCCAGTTCGGCACCTATATAGAAGCGCACCAACTCGACCAGCTTGCGGTAGCAGGCGGTGCCCGGTTGGAAGCGCTGGTAGTCGGCCAGGCTGAGCGGGCCCAGCTCGATGCGGa  <  3:245168/148‑1 (MQ=255)
                                                                                                                                           gCCCCGCGCCTCAAGCGCGGGGCAATTTCGAAATCCAGTTCGGCACCTATATAGAAGCGCACCAACTCGACCAGCTTGCGGTAGCAGGCGGTGCCCGGTTGGAAGCGCTGGTAGTCGGCCAGGCTGAGCGGGCCCAGCTCGATGCGGa  <  1:55792/148‑1 (MQ=255)
                                                                                                                                              |                                                                                                                                                
GGTTCACAAGGCGACCCCATCGTAAGGAATGTGGAAGACGGCGCAACGCGAAGGCTCCGCGTCACGACCAGGGCGTTTGAGCCAACCCAGCCAGCCCAGGGCGACATTGCCGCTGCGGCCCAGTTGGGCGCGTGGTACCGCCTCGCGCTTGAGGCGCGGGGCAATTTCGAAATCCAGTTCGGCACCTATATAGAAGCGCACCAACTCGACCAGCTTGCGGTAGCAGGCGGTGCCCGGCTGGAAGCGCTGGTAGTCGGCCAGGCTGAGCGGGCCCAGCTCGATGCGGA  >  NC_002947/3491345‑3491631

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑