Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	3,491,493	T→C	8.5%	K309R (AAG→AGG)	PP_3096 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	3,491,493	0	T	C	8.5%	195.6 / 6.4	70	K309R (AAG→AGG)	PP_3096	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (27/37);  new base C (3/3);  total (30/40)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.93e-01

GGTTCACAAGGCGACCCCATCGTAAGGAATGTGGAAGACGGCGCAACGCGAAGGCTCCGCGTCACGACCAGGGCGTTTGAGCCAACCCAGCCAGCCCAGGGCGACATTGCCGCTGCGGCCCAGTTGGGCGCGTGGTACCGCCTCGCGCTTGAGGCGCGGGGCAATTTCGAAATCCAGTTCGGCACCTATATAGAAGCGCACCAACTCGACCAGCTTGCGGTAGCAGGCGGTGCCCGGCTGGAAGCGCTGGTAGTCGGCCAGGCTGAGCGGGCCCAGCTCGATGCGGATGCACGAC  >  NC_002947/3491345‑3491639                                                                                                                                                     |                                                                                                                                                   ggTTCACAAGGCGACCCCATCGTAAGGAATGTGGAAGACGGCGCAACGCGAAGGCTCCGCGTCACGACCAGGGCGTTTGAGCCAACCCAGCCAGCCCAGGGCGACATTGCCGCTGCGGCCCAGTTGGGCGCGTGGTACCGCCTCGCGCt                                                                                                                                                    >  7:117632/1‑149 (MQ=255)  gTTCACAAGGCGACCCCATCGTAAGGAATGTGGAAGACGGCGCAACGCGAAGGCTCCGCGTCACGACCAGGGCGTTTGAGCCAACCCAGCCAGCCCAGGGCGACATTGCCGCTGCGGCCCAGTTGGGCGCGTGGTACCGCCTCGCGCtt                                                                                                                                                   <  4:149135/149‑1 (MQ=255)    tCACAAGGCGACCCCATCGTAAGGAATGTGGAAGACGGCGCAACGCGAAGGCTCCGCGTCACGACCAGGGCGTTTGAGCCAACCCAGCCAGCCCAGGGCGACATTGCCGCTGCGGCCCAGTTGGGCGCGTGGTACCGCCTCGCGCtt                                                                                                                                                   >  1:109783/1‑147 (MQ=255)      acaAGGCGACCCCATCGTAAGGAATGTGGAAGACGGCGCAACGCGAAGGCTCCGCGTCACGACCAGGGCGTTTGAGCCATCCCAGCCAGCCCAGGGCGACATTGCCGCTGCGGCCCAGTTGGGCGCGTGGTACCGCCTCGCGCTTGAgg                                                                                                                                               >  1:239184/1‑149 (MQ=255)       caAGGCGACCCCATCGTAAGGAATGTGGAAGACGGCGCAACGCGAAGGCTCCGCGTCACGACCAGGGCGTTTGAGCCAACCCAGCCAGCCCAGGGCGACATTGCCGCTGCGGCCCAGTTGGGCGCGTGGTACCGCCTCGCGCTTGAGgc                                                                                                                                              <  8:32248/149‑1 (MQ=255)        aaGGCGACCCCATCGTAAGGAATGTGGAAGACGGCGCAACGCGAAGGCTCCGCGTCACGACCAGGGCGTTTGAGCCAACCCAGCCAGCCCAGGGCGACATTGCCGCTGCGGCCCAGTTGGGCGCGTGGTACCGCCTCGCGCTTGAGgcg                                                                                                                                             >  7:29421/1‑149 (MQ=255)           gCGACCCCATCGTAAGGAATGTGGAAGACGGCGCAACGCGAAGGCTCCGCGTCACGACCAGGGCGTTTGAGCCAACCCAGCCAGCCCAGGGCGACATTGCCGCTGCGGCCCAGTTGGGCGCGTGGTACCGCCTCGCGCTTGAGGCGCgg                                                                                                                                          >  5:90215/1‑149 (MQ=255)            cGACCCCATCGTAAGGAATGTGGAAGACGGCGCAACGCGAAGGCTCCGCGTCACGACCAGGGCGTTTGAGCCATCCCAGCCAGCCCAGGGCGACATTGCCGCTGCGGCCCAGTTGGGCGCGTGGTACCGCCTCGCGCTTGAGGCGCggg                                                                                                                                         <  2:239184/149‑1 (MQ=255)            cGACCCCATCGTAAGGAATGTGGAAGACGGCGCAACGCGAAGGCTCCGCGTCACGACCAGGGCGTTTGAGCCAACCCAGCCAGCCCAGGGCGACATTGCCGCTGCGGCCCAGTTGGGCGCGTGGTACCGCCTCGCGCTTGAGGCGCggg                                                                                                                                         <  1:17899/149‑1 (MQ=255)             gACCCCATCGTAAGGAATGTGGAAGACGGCGCAACGCGAAGGCTCCGCGTCACGACCAGGGCGTTTGAGCCAACCCAGCCAGCCCAGGGCGACATTGCCGCTGCGGCCCAGTTGGGCGCGTGGTACCGCCTCGCGCTTGAGGCGCgggg                                                                                                                                        >  3:316001/1‑149 (MQ=255)              aCCCCATCGTAAGGAATGTGGAAGACGGCGCAACGCGAAGGCTCCGCGTCACGACCAGGGCGTTTGAGCCAACCCAGCCAGCCCAGGGCGACATTGCCGCTGCGGCCCAGTTGGGCGCGTGGTACCGCCTCGCGCTTGAGGCGCGGGGc                                                                                                                                       <  1:16415/149‑1 (MQ=255)                cccATCGTAAGGAATGTGGAAGACGGCGCAACGCGAAGGCTCCGCGTCACGACCAGGGCGTTTGAGCCAACCCAGCCAGCCCAGGGCGACATTGCCGCTGCGGCCCAGTTGGGCGCGTGGTACCGCCTCGCGCTTGAGGCGCGGGGCaa                                                                                                                                     >  8:64694/1‑149 (MQ=255)                cccATCGTAAGGAATGTGGAAGACGGCGCAACGCGAAGGCTCCGCGTCACGACCAGGGCGTTTGAGCCAACCCAGCCAGCCCAGGGCGACATTGCCGCTGCGGCCCAGTTGGGCGCGTGGTACCGCCCCGCGCCTCAAGCGCGAGGcgg                                                                                                                                     <  7:40302/149‑3 (MQ=255)                  cATCGTAAGGAATGTGGAAGACGGCGCAACGCGAAGGCTCCGCGTCACGACCAGGGCGTTTGAGCCAACCCAGCCAGCCCAGGGCGACATTGCCGCTGCGGCCCAGTTGGGCGCGTGGTACCGCCTCGCGCTTGAGGCGCGGGGCAAtt                                                                                                                                   <  8:117632/149‑1 (MQ=255)                  cATCGTAAGGAATGTGGAAGACGGCGCAACGCGAAGGCTCCGCGTCACGACCAGGGCGTTTGAGCCAACCCAGCCAGCCCAGGGCGACATTGCCGCTGCGGCCCAGTTGGGCGCGTGGTACCGCCTCGCGCTTGAGGCGCGGGGCAAtt                                                                                                                                   >  1:6389/1‑149 (MQ=255)                    tCGTAAGGAATGTGGAAGACGGCGCAACGCGAAGGCTCCGCGTCACGACCAGGGCGTTTGAGCCAACCCAGCCAGCCCAGGGCGACATTGCCGCTGCGGCCCAGTTGGGCGCGTGGTACCGCCTCGCGCTTGAGGCGCGGGGCAATTTc                                                                                                                                 <  6:133963/149‑1 (MQ=255)                    tCGTAAGGAATGTGGAAGACGGCGCAACGCGAAGGCTCCGCGTCACGACCAGGGCGTTTGAGCCAACCCAGCCAGCCCAGGGCGACATTGCCGCTGCGGCCCAGTTGGGCGCGTGGTACCGCCTCGCGCTTGAGGCGCGGGGCAATTTc                                                                                                                                 >  1:112199/1‑149 (MQ=255)                      gTAAGGAATGTGGAAGACGGCGCAACGCGAAGGCTCCGCGTCACGACCAGGGCGTTTGAGCCAACCCAGCCAGCCCAGGGCGACATTGCCGCTGCGGCCCAGTTGGGCGCGTGGTACCGCCTCGCGCTTGAGGCGCGGGGCAATTTCg                                                                                                                                <  3:50178/148‑1 (MQ=255)                       tAAGGAATGTGGAAGACGGCGCAACGCGAAGGCTCCGCGTCACGACCAGGGCGTTTGAGCCAACCCAGCCAGCCCAGGGCGACATTGCCGCTGCGGCCCAGTTGGGCGCGTGGTACCGCCTCGCGCTTGAGGCGCGGGGCAATTTCGaa                                                                                                                              <  7:67668/149‑1 (MQ=255)                       tAAGGAATGTGGAAGACGGCGCAACGCGAAGGCTCCGCGTCACGACCAGGGCGTTTGAGCCAACCCAGCCAGCCCAGGGCGACATTGCCGCTGCGGCCCAGTTGGGCGCGTGGTACCGCCTCGCGCTTGAGGCGCGGGGCAATTTCGa                                                                                                                               >  6:106414/1‑148 (MQ=255)                            aaTGTGGAAGACGGCGCAACGCGAAGGCTCCGCGTCACGACCAGGGCGTTTGAGCCAACCCAGCCAGCCCAGGGCGACATTGCCGCTGCGGCCCAGTTGGGCGCGTGGTACCGCCTCGCGCTTGAGGCGCGGGGCAATTTCGAAATCCa                                                                                                                         <  5:136924/149‑1 (MQ=255)                             aTGTGGAAGACGGCGCAACGCGAAGGCTCCGCGTCACGACCAGGGCGTTTGAGCCAACCCAGCCAGCCCAGGGCGACATTGCCGCTGCGGCCCAGTTGGGCGCGTGGTACCGCCTCGCGCTTGAGGCGCGGGGCAATTTCGAAATCCAg                                                                                                                        <  6:109445/149‑1 (MQ=255)                             aTGTGGAAGACGGCGCAACGCGAAGGCTCCGCGTCACGACCAGGGCGTTTGAGCCAACCCAGCCAGCCCAGGGCGACATTGCCGCTGCGGCCCAGTTGGGCGCGTGGTACCGCCTCGCGCTTGAGGCGCGGGGCAATTTCGAAATCCAg                                                                                                                        <  7:128118/149‑1 (MQ=255)                                 ggAAGACGGCGCAACGCGAAGGCTCCGCGTCACGACCAGGGCGTTTGAGCCAACCCAGCCAGCCCAGGGCGACATTGCCGCTGCGGCCCAGTTGGGCGCGTGGTACCGCCTCGCGCTTGAGGCGCGGGGCAATTTCGAAATCCAGTTCg                                                                                                                    >  4:326638/1‑149 (MQ=255)                                  gAAGACGGCGCAACGCGAAGGCTCCGCGTCACGACCAGGGCGTTTGAGCCAACCCAGCCAGCCCAGGGCGACATTGCCGCTGCGGCCCAGTTGGGCGCGTGGTACCGCCTCGCGCTTGAGGCGCGGGGCAATTTCGAAATCCAGTTCg                                                                                                                    <  7:64201/148‑1 (MQ=255)                                   aaGACGGCGCAACGCGAAGGCTCCGCGTCACGACCAGGGCGTTTGAGCCAACCCAGCCAGCCCAGGGCGACATTGCCGCTGCGGCCCAGTTGGGCGCGTGGTACCGCCTCGCGCTTGAGGCGCGGGGCAATTTCGAAATCCAGTTCGGc                                                                                                                  <  2:91679/149‑1 (MQ=255)                                       cGGCGCAACGCGAAGGCTCCGCGTCACGACCAGGGCGTTTGAGCCAACCCAGCCAGCCCAGGGCGACATTGCCGCTGCGGCCCAGTTGGGCGCGTGGTACCGCCTCGCGCTTGAGGCGCGGGGCAATTTCGAAATCCAGTTCGGCACCt                                                                                                              <  2:6389/149‑1 (MQ=255)                                       cGGCGCAACGCGAAGGCTCCGCGTCACGACCAGGGCGTTTGAGCCAACCCAGCCAGCCCAGGGCGACATTGCCGCTGCGGCCCAGTTGGGCGCGTGGTACCGCCTCGCGCTTGAGGCGCGGGGCAATTTCGAAATCCAGTTCGGCACCt                                                                                                              <  7:38768/149‑1 (MQ=255)                                        ggCGCAACGCGAAGGCTCCGCGTCACGACCAGGGCGTTTGAGCCAACCCAGCCAGCCCAGGGCGACATTGCCGCTGCGGCCCAGTTGGGCGCGTGGTACCGCCTCGCGCTTGAGGCGCGGGGCAATTTCGAAATCCAGTTCGGCACCta                                                                                                             >  6:249620/1‑149 (MQ=255)                                          cgcAACGCGAAGGCTCCGCGTCACGACCAGGGCGTTTGAGCCAACCCAGCCAGCCCAGGGCGACATTGCCGCTGCGGCCCAGTTGGGCGCGTGGTACCGCCTCGCGCTTGAGGCGCGGGGCAATTTCGAAATCCAGTTCGGCACCtata                                                                                                           <  2:109783/149‑1 (MQ=255)                                           gcAACGCGAAGGCTCCGCGTCACGACCAGGGCGTTTGAGCCAACCCAGCCAGCCCAGGGCGACATTGCCGCTGCGGCCCAGTTGGGCGCGTGGTACCGCCTCGCGCTTGAGGCGCGGGGCAATTTCGAAATCCAGTTCGGCACCtata                                                                                                           >  1:276743/1‑148 (MQ=255)                                           gcAACGCGAAGGCTCCGCGTCACGACCAGGGCGTTTGAGCCAACCCAGCCAGCCCAGGGCGACATTGCCGCTGCGGCCCAGTTGGGCGCGTGGTACCGCCTCGCGCTGGAGGCGCGGGGCAATTTCGAAATCCAGTTCGGCACCtata                                                                                                           >  4:89006/1‑148 (MQ=255)                                               cgcgAAGGCTCCGCGTCACGACCAGGGCGTTTGAGCCAACCCAGCCAGCCCAGGGCGACATTGCCGCTGCGGCCCAGTTGGGCGCGTGGTACCGCCTCGCGCTTGAGGCGCGGGGCAATTTCGAAATCCAGTTCGGCACCTATATAGaa                                                                                                      <  8:29421/149‑1 (MQ=255)                                                 cgAAGGCTCCGCGTCACGACCAGGGCGTTTGAGCCAACCCAGCCAGCCCAGGGCGACATTGCCGCTGCGGCCCAGTTGGGCGCGTGGTACCGCCTCGCGCTTGAGGCGCGGGGCAATTTCGAAATCCAGTTCGGCACCTATATAGAAgc                                                                                                    <  7:24131/149‑1 (MQ=255)                                                 cgAAGGCTCCGCGTCACGACCAGGGCGTTTGAGCCAACCCAGCCAGCCCAGGGCGACATTGCCGCTGCGGCCCAGTTGGGCGCGTGGTACCGCCTCGCGCTTGAGGCGCGGGGCAATTTCGAAATCCAGTTCGGCACCTATATAGAAgc                                                                                                    <  7:83614/149‑1 (MQ=255)                                                        tCCGCGTCACGACCAGGGCGTTTGAGCCAACCCAGCCAGCCCAGGGCGACATTGCCGCTGCGGCCCAGTTGGGCGCGTGGTACCGCCTCGCGCTTGAGGCGCGGGGCAATTTCGAAATCCAGTTCGGCACCTATATAGAAGCGCACCaa                                                                                             >  5:333373/1‑149 (MQ=255)                                                             gTCACGACCAGGGCGTTTGAGCCAACCCAGCCAGCCCAGGGCGACATTGCCGCTGCGGCCCAGTTGGGCGCGTGGTACCGCCTCGCGCTTGAGGCGCGGGGCAATTTCGAAATCCAGTTCGGCACCTATATAGAAGCGCACCAACTCGa                                                                                        <  3:326638/149‑1 (MQ=255)                                                              tCACGACCAGGGCGTTTGAGCCAACCCAGCCAGCCCAGGGCGACATTGCCGCTGCGGCCCAGTTGGGCGCGTGGTACCGCCTCGCGCTTGAGGCGCGGGGCAATTTCGAAATCCAGTTCGGCACCTATATAGCAGCGCACCAACTCGAc                                                                                       <  4:316001/149‑1 (MQ=255)                                                              tCACGACCAGGGCGTTTGAGCCAACCCAGCCAGCCCAGGGCGACATTGCCGCTGCGGCCCAGTTAGGCGCGTGGTACCGCCTCGCGCTTGAGGCGCGGGGCAATTTCGAAATCCAGTTCGGCACCTATATAGAAGCGCACCAACTCGAc                                                                                       >  7:204935/1‑149 (MQ=255)                                                               cACGACCAGGGCGTTTGAGCCAACCCAGCCAGCCCAGGGCGACATTGCCGCTGCGGCCCAGTTGGGCGCGTGGTACCGCCTCGCGCTTGAGGCGCGGGGCAATTTCGAAATCCAGTTCGGCACCTATATAGAAGCGCACCAACTCGAc                                                                                       >  2:340404/1‑148 (MQ=255)                                                                 cGACCAGGGAGTTTGAGCCAACCCAGCCAGCCCAGCGCGACATTGCCGCTGCGGCCCAGTTGGGCGCGTGGTACCGCCTCGCGCTTGAGGCGCGGGGCAAATTCGAAAACCAGTTCGGCACCTATATAGAAGCGCACCAACTCCAccc                                                                                     >  6:76227/1‑147 (MQ=255)                                                                  gACCAGGGCGTTTGAGCCAACCCAGCCAGCCCAGGGCGACATTGCCGCTGCGGCCCAGTAGGGCGCGTGGTACCGCCTCGCGCTAGAGGCGCGCGGCAAATTCGAAATCCAGTAAGGCACCTATATAGAAGCCCACCAACTCGACCAGc                                                                                   >  2:117505/1‑149 (MQ=255)                                                                    ccAGGGCGTTTGAGCCAACCCAGCCAGCCCAGGGCGACATTGCCGCTGCGGCCCAGTTGGGCGCGTGGTACCGCCTCGCGCTTGAGGcgcg                                                                                                                                           >  2:314141/1‑91 (MQ=255)                                                                    ccAGGGCGTTTGAGCCAACCCAGCCAGCCCAGGGCGACATTGCCGCTGCGGCCCAGTTGGGCGCGTGGTACCGCCTCGCGCTTGAGGcgcg                                                                                                                                           <  1:314141/91‑1 (MQ=255)                                                                     cAGGGCGTTTGAGCTATCCCAGCCAGCCCAGGGCGACATTGCCGCTGCGGCCCAGTTGGGCGCGTGGTACCGCCTCGCGCTTGAGGCGCGGGGCAATTTCGAAATCCAGTTCGGCACCTATATAGAAGCGCACCAACTCGACCAGCTTg                                                                                <  1:117505/149‑1 (MQ=255)                                                                        ggCGTTTGAGCCAACCCAGCCAGCCCAGGGCGACATTGCCGCTGCGGCCCAGTTGGGCGCGTGGTACCGCCTCGCGCTTGAGGCGCGGGGCAATTTCGAAATCCAGTTCGGCACCTATATAGAAGCGCACCAACTCGACCAGCTTGCgg                                                                             <  5:106414/149‑1 (MQ=255)                                                                        ggCGTTTGAGCCAACCCAGCCAGCCCAGGGCGACATTGCCGCTGCGGCCCAGTTGGGCGCGTGGTACCGCCTCGCGCTTGAGGCGCGGGGCAATTTCGAAATCCAGTTCGGCACCTATATAGAAGCGCACCAACTCGACCAGCTTGCgg                                                                             <  6:333373/149‑1 (MQ=255)                                                                          cGTTTGAGCCAACCCAGCCAGCCCAGGGCGACATTGCCGCTGCGGCCCAGTTGGGCGCGTGGTACCGCCTCGCGCTTGAGGCGCGGGGCAATTTCGAAATCCAGTTCGGCACCTATATAGAAGCGCACCAACTCGACCAGCTTGCGGTa                                                                           <  2:112199/149‑1 (MQ=255)                                                                                 gCCAACCCAGCCAGCCCAGGGCGACATTGCCGCTGCGGCCCAGTTGGGCGCGTGGTACCGCCTCGCGCTTGAGGCGCGGGGCAATTTCGAAATCCAGTTCGGCACCTATATAGAAGCGCACCAACTCGACCAGCTTGCGGTAGCAGGCg                                                                    >  5:200409/1‑149 (MQ=255)                                                                                  ccAACCCAGCCAGCCCAGGGCGACATTGCCGCTGCGGCCCAGTTGGGCGCGTGGTACCGCCTCGCGCTTGAGGCGCGGGGCAATTTCGAAATCCAGTTCGGCACATATATAGAAGCGCACAAACTCGACCAGCTTGCGGTAGCAGGCgt                                                                   >  6:324293/1‑148 (MQ=255)                                                                                   cAACCCAGCCAGCCCAGGGCGACATTGCCGCTGCGGCCCAGTTGGGCGCGTGGTACCGCCTCGCGCTTGAGGCGCGGGGCAATTTCGAAATCCAGTTCGGCACCTATATAGAAGCGCACCAACTCGACCAGCTTGCGGTAGCAGGCGGt                                                                  <  2:81732/149‑1 (MQ=255)                                                                                   cAACCCAGCCAGCCCAGGGCGACATTGCCGCTGCGGCCCAGTTGGGCGCGTGGTACCGCCTAGCCCTTCAGCCCCGGGGCAAATTCGAAATACAGATCGGCACCTATATAGAAGCGCACCAACTCGACCAGCTAGCGGTAGCAGCCGGt                                                                  >  8:58383/1‑149 (MQ=255)                                                                                     aCCCAGCCAGCCCAGGGCGACATTGCCGCTGCGGCCCAGTTGGGCGCGTGGTACCGCCTCGCGCTTGAGGCGCGGGGCAATTTCGAAATCCAGTTCGGCACCTATATAGAAGCGCACCAACTCGACCAGCTTGCGGTAGCAGGCGGTGc                                                                >  5:273640/1‑149 (MQ=255)                                                                                       ccagccagCCCAGGGCGACATTGCCGCTGCGGCCCAGTTGGGCGCGTGGTACCGCCTCGCGCTTGAGGCGCGGGGCAATTTCGAAATCCAGTTCGGCACCTATATAGAAGCGCACCAACTCGACCAGCTTGCGGTAGCAGGCGGTGccc                                                              >  7:136696/1‑149 (MQ=255)                                                                                             agccCAGGGCGACATTGCCGCTGCGGCCCAGTTGGGCGCGTGGTACCGCCTCGCGCTTGAGGCGCGGGGCAATTTCGAAATCCAGTTCGGCACCTATATAGAAGCGCACCAACTCGACCAGCTTGCGGTAGCAGGCGGTGCCCGGATg                                                         >  3:284268/1‑148 (MQ=255)                                                                                               cccAGGGCGACATTGCCGCTGCGGCCCAGTTGGGCGCGTGGTACCGCCTCGCGCTTGAGGCGCGGGGCAATTTCGAAATCCAGTTCGGCACCTATATAGAAGCGCACCAACTCGACCAGCTTGCGGTAGCAGGCGGTGCCCGGCTGGaa                                                      <  8:136696/149‑1 (MQ=255)                                                                                                      cGACATTGCCGCTGCGGCCCAGTTGGGCGCGTGGTACCGCCTCGCGCTTGAGGCGCGGGGCAATTTCGAAATCCAGTTCGGCACCTATATAGAAGCGCACCAACTCGACCAGCTTGCGGTAGCAGGCGGTGCCCGGCTGGAAGCGCTgg                                               <  6:90215/149‑1 (MQ=255)                                                                                                      cGACATTGCCGCTGCGGCCCAGTTGGGCGCGTGGTACCGCCTCGCGCTTGAGGCGCGGGGCAATTTCGAAATCCAGTTCGGCACCTATATAGAAGCGCACCAACTCGACCAGCTTGCGGTAGCAGGCGGTGCCCGGCTGGAAGCGCTgg                                               <  7:64694/149‑1 (MQ=255)                                                                                                              ccGCTGCGGCCCAGGTGGGCGCGTGGTACCGCCTCGCGCTTGAGGCGCGGGGCAATTTCGAAATCCAGTTCGGCACCTATATAGAAGCGCACCAACTCGACCAGCTTGCGGTAGCAGGCGGTGCCCGGCTGGAAGCGCTGGTAGTCGGc                                       <  8:216303/149‑1 (MQ=255)                                                                                                                gCTGCGGCCCAGTTGGGCGCGTGGTACCGCCTCGCGCTTGAGGCGCGGGGCAATTTCGAAATCCAGTTCGGCACCTATATAGAAGCGCACCAACTCGACCAGCTTGCGGTTGCAGTCTGTGCCAGGCTTGAAGCGCTGGTAGTCGGCCa                                     <  5:76227/149‑1 (MQ=255)                                                                                                                    cGGCCCAGTTGGGCGCGTGGTACCGCCTCGCGCTTGAGGCGCGGGGCAATTTCGAAATCCAGTTCGGCACCTATATAGAAGCGCACCAACTCGACCAGCTTGCGGTAGCAGGCGGTGCCCGGCTGGAAGCGCTGGTAGTCGGCCAGGc                                  <  1:4909/148‑1 (MQ=255)                                                                                                                           gTTGGGCGCGTGGTACCGCCTCGCGCTTGAGGCGCGGGGCAATTTCGAAATCCAGTTCGGCACCTATATAGAAGCGCACCAACTCGACCAGc                                                                                   <  1:86768/92‑1 (MQ=255)                                                                                                                           gTTGGGCGCGTGGTACCGCCTCGCGCTTGAGGCGCGGGGCAATTTCGAAATCCAGTTCGGCACCTATATAGAAGCGCACCAACTCGACCAGc                                                                                   >  2:86768/1‑92 (MQ=255)                                                                                                                                   cgTGGTACCGCCTCGCGCTTGAGGCGCGGGGCAATTTCGAAATCCAGTTCGGCACCTATATAGAAGCGCACCAACTCGACCAGCTTGCGGTAGCAGGCGGTGCCCGGCTg                                                         >  6:189588/1‑110 (MQ=255)                                                                                                                                   cgTGGTACCGCCTCGCGCTTGAGGCGCGGGGCAATTTCGAAATCCAGTTCGGCACCTATATAGAAGCGCACCAACTCGACCAGCTTGCGGTAGCAGGCGGTGCCCGGCTg                                                         <  5:189588/110‑1 (MQ=255)                                                                                                                                      gaaattgCCCCGCGCCTCAAGCGCGGGGCAATTTCGAAATCCAGTTCGGCACCTATATAGAAGCGCACCAACTCGACCAGCTTGCGGTAGCAGGCGGTGCCCGGTTGGAAGCGCTGGTAGTCGGCCAGGCTGAGCGGGCCCAGCTCGAt               >  2:55792/7‑149 (MQ=255)                                                                                                                                      gaaattgCCCCGCGCCTCAAGCGCGGGGCAATTTCGAAATCCAGTTCGGCACCTATATAGAAGCGCACCAACTCGACCAGCTTGCGGTAGCAGGCGGTGCCCGGTTGGAAGCGCTGGTAGTCGGCCAGGCTGAGCGGGCCCAGCTCGAt               >  4:245168/7‑149 (MQ=255)                                                                                                                                        tACCGCCTCGCGCTTGAGGCGCGGGGCAATTTCGAAATCCAGTTCGGCACCTATATAGAAGCGCACCAACTCGACCAGCTTGCGGTAGCAGGCGGTGCCCGGCTGGAAGCGCTGGTAGTCGGCCAGGCTGAGCGGGCCCAGCTCGATGc             <  1:340404/149‑1 (MQ=255)                                                                                                                                        tACCGCCTCGCGCTTGAGGCGCGGGGCAATTTCGAAATCCAGTTCGGCACCTATATAGAAGCGCACCAACTCGACCAGCTTGCGGTAGCAGGCGGTGCCCGGCTGGAAGCGCTGGTAGTCGGCCAGGCTGAGCGGGCCCAGCTCGATGc             >  6:309484/1‑149 (MQ=255)                                                                                                                                            gCCCCGCGCCTCAAGCGCGGGGCAATTTCGAAATCCAGTTCGGCACCTATATAGAAGCGCACCAACTCGACCAGCTTGCGGTAGCAGGCGGTGCCCGGTTGGAAGCGCTGGTAGTCGGCCAGGCTGAGCGGGCCCAGCTCGATGCGGa          <  3:245168/148‑1 (MQ=255)                                                                                                                                            gCCCCGCGCCTCAAGCGCGGGGCAATTTCGAAATCCAGTTCGGCACCTATATAGAAGCGCACCAACTCGACCAGCTTGCGGTAGCAGGCGGTGCCCGGTTGGAAGCGCTGGTAGTCGGCCAGGCTGAGCGGGCCCAGCTCGATGCGGa          <  1:55792/148‑1 (MQ=255)                                                                                                                                             ccccgcgCCTCAAGCGCGGGGCAATTTCGAAATCCAGTTCGGCACCTATATAGAAGCGCACCAACTCGACCAGCTTGCGGTAGCAGGCGGTGCCCGGCTGGAAGCCCTGGTAGTCGGCCAGGCTGAGCGGGCCCAGCTCGATGCGGATg        >  1:13000/4‑149 (MQ=255)                                                                                                                                                   gcTTGAGGCGCGGGGCAATTTCGAAATCCAGTTCGGCACCTATATAGAAGCGCACCAACTCGACCAGCTTGCGGTAGCAGGCGGTGCCCGGCTGGAAGCGCTGGTAGTCGGCCAGGCTGAGCGGGCCCAGCTCGATGCGGATGCACGAc  <  5:324293/149‑1 (MQ=255)                                                                                                                                                     |                                                                                                                                                   GGTTCACAAGGCGACCCCATCGTAAGGAATGTGGAAGACGGCGCAACGCGAAGGCTCCGCGTCACGACCAGGGCGTTTGAGCCAACCCAGCCAGCCCAGGGCGACATTGCCGCTGCGGCCCAGTTGGGCGCGTGGTACCGCCTCGCGCTTGAGGCGCGGGGCAATTTCGAAATCCAGTTCGGCACCTATATAGAAGCGCACCAACTCGACCAGCTTGCGGTAGCAGGCGGTGCCCGGCTGGAAGCGCTGGTAGTCGGCCAGGCTGAGCGGGCCCAGCTCGATGCGGATGCACGAC  >  NC_002947/3491345‑3491639

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑