Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	3,491,495	G→C	9.7%	L308L (CTC→CTG) ‡	PP_3096 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	3,491,495	0	G	C	9.7%	211.9 / 9.3	70	L308L (CTC→CTG) ‡	PP_3096	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (26/37);  new base C (4/3);  total (30/40)
Fisher's exact test for biased strand distribution p-value = 4.52e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ACAAGGCGACCCCATCGTAAGGAATGTGGAAGACGGCGCAACGCGAAGGCTCCGCGTCACGACCAGGGCGTTTGAGCCAACCCAGCCAGCCCAGGGCGACATTGCCGCTGCGGCCCAGTTGGGCGCGTGGTACCGCCTCGCGCTTGAGGCGCGGGGCAATTTCGAAATCCAGTTCGGCACCTATATAGAAGCGCACCAACTCGACCAGCTTGCGGTAGCAGGCGGTGCCCGGCTGGAAGCGCTGGTAGTCGGCCAGGCTGAGCGGGCCCAGCTCGATGCGGATGCACGACTGG  >  NC_002947/3491350‑3491642                                                                                                                                                  |                                                                                                                                                    acaAGGCGACCCCATCGTAAGGAATGTGGAAGACGGCGCAACGCGAAGGCTCCGCGTCACGACCAGGGCGTTTGAGCCATCCCAGCCAGCCCAGGGCGACATTGCCGCTGCGGCCCAGTTGGGCGCGTGGTACCGCCTCGCGCTTGAgg                                                                                                                                                  >  1:239184/1‑149 (MQ=255)  caAGGCGACCCCATCGTAAGGAATGTGGAAGACGGCGCAACGCGAAGGCTCCGCGTCACGACCAGGGCGTTTGAGCCAACCCAGCCAGCCCAGGGCGACATTGCCGCTGCGGCCCAGTTGGGCGCGTGGTACCGCCTCGCGCTTGAGgc                                                                                                                                                 <  8:32248/149‑1 (MQ=255)   aaGGCGACCCCATCGTAAGGAATGTGGAAGACGGCGCAACGCGAAGGCTCCGCGTCACGACCAGGGCGTTTGAGCCAACCCAGCCAGCCCAGGGCGACATTGCCGCTGCGGCCCAGTTGGGCGCGTGGTACCGCCTCGCGCTTGAGgcg                                                                                                                                                >  7:29421/1‑149 (MQ=255)      gCGACCCCATCGTAAGGAATGTGGAAGACGGCGCAACGCGAAGGCTCCGCGTCACGACCAGGGCGTTTGAGCCAACCCAGCCAGCCCAGGGCGACATTGCCGCTGCGGCCCAGTTGGGCGCGTGGTACCGCCTCGCGCTTGAGGCGCgg                                                                                                                                             >  5:90215/1‑149 (MQ=255)       cGACCCCATCGTAAGGAATGTGGAAGACGGCGCAACGCGAAGGCTCCGCGTCACGACCAGGGCGTTTGAGCCATCCCAGCCAGCCCAGGGCGACATTGCCGCTGCGGCCCAGTTGGGCGCGTGGTACCGCCTCGCGCTTGAGGCGCggg                                                                                                                                            <  2:239184/149‑1 (MQ=255)       cGACCCCATCGTAAGGAATGTGGAAGACGGCGCAACGCGAAGGCTCCGCGTCACGACCAGGGCGTTTGAGCCAACCCAGCCAGCCCAGGGCGACATTGCCGCTGCGGCCCAGTTGGGCGCGTGGTACCGCCTCGCGCTTGAGGCGCggg                                                                                                                                            <  1:17899/149‑1 (MQ=255)        gACCCCATCGTAAGGAATGTGGAAGACGGCGCAACGCGAAGGCTCCGCGTCACGACCAGGGCGTTTGAGCCAACCCAGCCAGCCCAGGGCGACATTGCCGCTGCGGCCCAGTTGGGCGCGTGGTACCGCCTCGCGCTTGAGGCGCgggg                                                                                                                                           >  3:316001/1‑149 (MQ=255)         aCCCCATCGTAAGGAATGTGGAAGACGGCGCAACGCGAAGGCTCCGCGTCACGACCAGGGCGTTTGAGCCAACCCAGCCAGCCCAGGGCGACATTGCCGCTGCGGCCCAGTTGGGCGCGTGGTACCGCCTCGCGCTTGAGGCGCGGGGc                                                                                                                                          <  1:16415/149‑1 (MQ=255)           cccATCGTAAGGAATGTGGAAGACGGCGCAACGCGAAGGCTCCGCGTCACGACCAGGGCGTTTGAGCCAACCCAGCCAGCCCAGGGCGACATTGCCGCTGCGGCCCAGTTGGGCGCGTGGTACCGCCTCGCGCTTGAGGCGCGGGGCaa                                                                                                                                        >  8:64694/1‑149 (MQ=255)           cccATCGTAAGGAATGTGGAAGACGGCGCAACGCGAAGGCTCCGCGTCACGACCAGGGCGTTTGAGCCAACCCAGCCAGCCCAGGGCGACATTGCCGCTGCGGCCCAGTTGGGCGCGTGGTACCGCCCCGCGCCTCAAGCGCGAGGcgg                                                                                                                                        <  7:40302/149‑3 (MQ=255)             cATCGTAAGGAATGTGGAAGACGGCGCAACGCGAAGGCTCCGCGTCACGACCAGGGCGTTTGAGCCAACCCAGCCAGCCCAGGGCGACATTGCCGCTGCGGCCCAGTTGGGCGCGTGGTACCGCCTCGCGCTTGAGGCGCGGGGCAAtt                                                                                                                                      >  1:6389/1‑149 (MQ=255)             cATCGTAAGGAATGTGGAAGACGGCGCAACGCGAAGGCTCCGCGTCACGACCAGGGCGTTTGAGCCAACCCAGCCAGCCCAGGGCGACATTGCCGCTGCGGCCCAGTTGGGCGCGTGGTACCGCCTCGCGCTTGAGGCGCGGGGCAAtt                                                                                                                                      <  8:117632/149‑1 (MQ=255)               tCGTAAGGAATGTGGAAGACGGCGCAACGCGAAGGCTCCGCGTCACGACCAGGGCGTTTGAGCCAACCCAGCCAGCCCAGGGCGACATTGCCGCTGCGGCCCAGTTGGGCGCGTGGTACCGCCTCGCGCTTGAGGCGCGGGGCAATTTc                                                                                                                                    >  1:112199/1‑149 (MQ=255)               tCGTAAGGAATGTGGAAGACGGCGCAACGCGAAGGCTCCGCGTCACGACCAGGGCGTTTGAGCCAACCCAGCCAGCCCAGGGCGACATTGCCGCTGCGGCCCAGTTGGGCGCGTGGTACCGCCTCGCGCTTGAGGCGCGGGGCAATTTc                                                                                                                                    <  6:133963/149‑1 (MQ=255)                 gTAAGGAATGTGGAAGACGGCGCAACGCGAAGGCTCCGCGTCACGACCAGGGCGTTTGAGCCAACCCAGCCAGCCCAGGGCGACATTGCCGCTGCGGCCCAGTTGGGCGCGTGGTACCGCCTCGCGCTTGAGGCGCGGGGCAATTTCg                                                                                                                                   <  3:50178/148‑1 (MQ=255)                  tAAGGAATGTGGAAGACGGCGCAACGCGAAGGCTCCGCGTCACGACCAGGGCGTTTGAGCCAACCCAGCCAGCCCAGGGCGACATTGCCGCTGCGGCCCAGTTGGGCGCGTGGTACCGCCTCGCGCTTGAGGCGCGGGGCAATTTCGaa                                                                                                                                 <  7:67668/149‑1 (MQ=255)                  tAAGGAATGTGGAAGACGGCGCAACGCGAAGGCTCCGCGTCACGACCAGGGCGTTTGAGCCAACCCAGCCAGCCCAGGGCGACATTGCCGCTGCGGCCCAGTTGGGCGCGTGGTACCGCCTCGCGCTTGAGGCGCGGGGCAATTTCGa                                                                                                                                  >  6:106414/1‑148 (MQ=255)                       aaTGTGGAAGACGGCGCAACGCGAAGGCTCCGCGTCACGACCAGGGCGTTTGAGCCAACCCAGCCAGCCCAGGGCGACATTGCCGCTGCGGCCCAGTTGGGCGCGTGGTACCGCCTCGCGCTTGAGGCGCGGGGCAATTTCGAAATCCa                                                                                                                            <  5:136924/149‑1 (MQ=255)                        aTGTGGAAGACGGCGCAACGCGAAGGCTCCGCGTCACGACCAGGGCGTTTGAGCCAACCCAGCCAGCCCAGGGCGACATTGCCGCTGCGGCCCAGTTGGGCGCGTGGTACCGCCTCGCGCTTGAGGCGCGGGGCAATTTCGAAATCCAg                                                                                                                           <  6:109445/149‑1 (MQ=255)                        aTGTGGAAGACGGCGCAACGCGAAGGCTCCGCGTCACGACCAGGGCGTTTGAGCCAACCCAGCCAGCCCAGGGCGACATTGCCGCTGCGGCCCAGTTGGGCGCGTGGTACCGCCTCGCGCTTGAGGCGCGGGGCAATTTCGAAATCCAg                                                                                                                           <  7:128118/149‑1 (MQ=255)                            ggAAGACGGCGCAACGCGAAGGCTCCGCGTCACGACCAGGGCGTTTGAGCCAACCCAGCCAGCCCAGGGCGACATTGCCGCTGCGGCCCAGTTGGGCGCGTGGTACCGCCTCGCGCTTGAGGCGCGGGGCAATTTCGAAATCCAGTTCg                                                                                                                       >  4:326638/1‑149 (MQ=255)                             gAAGACGGCGCAACGCGAAGGCTCCGCGTCACGACCAGGGCGTTTGAGCCAACCCAGCCAGCCCAGGGCGACATTGCCGCTGCGGCCCAGTTGGGCGCGTGGTACCGCCTCGCGCTTGAGGCGCGGGGCAATTTCGAAATCCAGTTCg                                                                                                                       <  7:64201/148‑1 (MQ=255)                              aaGACGGCGCAACGCGAAGGCTCCGCGTCACGACCAGGGCGTTTGAGCCAACCCAGCCAGCCCAGGGCGACATTGCCGCTGCGGCCCAGTTGGGCGCGTGGTACCGCCTCGCGCTTGAGGCGCGGGGCAATTTCGAAATCCAGTTCGGc                                                                                                                     <  2:91679/149‑1 (MQ=255)                                  cGGCGCAACGCGAAGGCTCCGCGTCACGACCAGGGCGTTTGAGCCAACCCAGCCAGCCCAGGGCGACATTGCCGCTGCGGCCCAGTTGGGCGCGTGGTACCGCCTCGCGCTTGAGGCGCGGGGCAATTTCGAAATCCAGTTCGGCACCt                                                                                                                 <  7:38768/149‑1 (MQ=255)                                  cGGCGCAACGCGAAGGCTCCGCGTCACGACCAGGGCGTTTGAGCCAACCCAGCCAGCCCAGGGCGACATTGCCGCTGCGGCCCAGTTGGGCGCGTGGTACCGCCTCGCGCTTGAGGCGCGGGGCAATTTCGAAATCCAGTTCGGCACCt                                                                                                                 <  2:6389/149‑1 (MQ=255)                                   ggCGCAACGCGAAGGCTCCGCGTCACGACCAGGGCGTTTGAGCCAACCCAGCCAGCCCAGGGCGACATTGCCGCTGCGGCCCAGTTGGGCGCGTGGTACCGCCTCGCGCTTGAGGCGCGGGGCAATTTCGAAATCCAGTTCGGCACCta                                                                                                                >  6:249620/1‑149 (MQ=255)                                     cgcAACGCGAAGGCTCCGCGTCACGACCAGGGCGTTTGAGCCAACCCAGCCAGCCCAGGGCGACATTGCCGCTGCGGCCCAGTTGGGCGCGTGGTACCGCCTCGCGCTTGAGGCGCGGGGCAATTTCGAAATCCAGTTCGGCACCtata                                                                                                              <  2:109783/149‑1 (MQ=255)                                      gcAACGCGAAGGCTCCGCGTCACGACCAGGGCGTTTGAGCCAACCCAGCCAGCCCAGGGCGACATTGCCGCTGCGGCCCAGTTGGGCGCGTGGTACCGCCTCGCGCTTGAGGCGCGGGGCAATTTCGAAATCCAGTTCGGCACCtata                                                                                                              >  1:276743/1‑148 (MQ=255)                                      gcAACGCGAAGGCTCCGCGTCACGACCAGGGCGTTTGAGCCAACCCAGCCAGCCCAGGGCGACATTGCCGCTGCGGCCCAGTTGGGCGCGTGGTACCGCCTCGCGCTGGAGGCGCGGGGCAATTTCGAAATCCAGTTCGGCACCtata                                                                                                              >  4:89006/1‑148 (MQ=255)                                          cgcgAAGGCTCCGCGTCACGACCAGGGCGTTTGAGCCAACCCAGCCAGCCCAGGGCGACATTGCCGCTGCGGCCCAGTTGGGCGCGTGGTACCGCCTCGCGCTTGAGGCGCGGGGCAATTTCGAAATCCAGTTCGGCACCTATATAGaa                                                                                                         <  8:29421/149‑1 (MQ=255)                                            cgAAGGCTCCGCGTCACGACCAGGGCGTTTGAGCCAACCCAGCCAGCCCAGGGCGACATTGCCGCTGCGGCCCAGTTGGGCGCGTGGTACCGCCTCGCGCTTGAGGCGCGGGGCAATTTCGAAATCCAGTTCGGCACCTATATAGAAgc                                                                                                       <  7:83614/149‑1 (MQ=255)                                            cgAAGGCTCCGCGTCACGACCAGGGCGTTTGAGCCAACCCAGCCAGCCCAGGGCGACATTGCCGCTGCGGCCCAGTTGGGCGCGTGGTACCGCCTCGCGCTTGAGGCGCGGGGCAATTTCGAAATCCAGTTCGGCACCTATATAGAAgc                                                                                                       <  7:24131/149‑1 (MQ=255)                                                   tCCGCGTCACGACCAGGGCGTTTGAGCCAACCCAGCCAGCCCAGGGCGACATTGCCGCTGCGGCCCAGTTGGGCGCGTGGTACCGCCTCGCGCTTGAGGCGCGGGGCAATTTCGAAATCCAGTTCGGCACCTATATAGAAGCGCACCaa                                                                                                >  5:333373/1‑149 (MQ=255)                                                        gTCACGACCAGGGCGTTTGAGCCAACCCAGCCAGCCCAGGGCGACATTGCCGCTGCGGCCCAGTTGGGCGCGTGGTACCGCCTCGCGCTTGAGGCGCGGGGCAATTTCGAAATCCAGTTCGGCACCTATATAGAAGCGCACCAACTCGa                                                                                           <  3:326638/149‑1 (MQ=255)                                                         tCACGACCAGGGCGTTTGAGCCAACCCAGCCAGCCCAGGGCGACATTGCCGCTGCGGCCCAGTTGGGCGCGTGGTACCGCCTCGCGCTTGAGGCGCGGGGCAATTTCGAAATCCAGTTCGGCACCTATATAGCAGCGCACCAACTCGAc                                                                                          <  4:316001/149‑1 (MQ=255)                                                         tCACGACCAGGGCGTTTGAGCCAACCCAGCCAGCCCAGGGCGACATTGCCGCTGCGGCCCAGTTAGGCGCGTGGTACCGCCTCGCGCTTGAGGCGCGGGGCAATTTCGAAATCCAGTTCGGCACCTATATAGAAGCGCACCAACTCGAc                                                                                          >  7:204935/1‑149 (MQ=255)                                                          cACGACCAGGGCGTTTGAGCCAACCCAGCCAGCCCAGGGCGACATTGCCGCTGCGGCCCAGTTGGGCGCGTGGTACCGCCTCGCGCTTGAGGCGCGGGGCAATTTCGAAATCCAGTTCGGCACCTATATAGAAGCGCACCAACTCGAc                                                                                          >  2:340404/1‑148 (MQ=255)                                                            cGACCAGGGAGTTTGAGCCAACCCAGCCAGCCCAGCGCGACATTGCCGCTGCGGCCCAGTTGGGCGCGTGGTACCGCCTCGCGCTTGAGGCGCGGGGCAAATTCGAAAACCAGTTCGGCACCTATATAGAAGCGCACCAACTCCAccc                                                                                        >  6:76227/1‑147 (MQ=255)                                                             gACCAGGGCGTTTGAGCCAACCCAGCCAGCCCAGGGCGACATTGCCGCTGCGGCCCAGTAGGGCGCGTGGTACCGCCTCGCGCTAGAGGCGCGCGGCAAATTCGAAATCCAGTAAGGCACCTATATAGAAGCCCACCAACTCGACCAGc                                                                                      >  2:117505/1‑149 (MQ=255)                                                               ccAGGGCGTTTGAGCCAACCCAGCCAGCCCAGGGCGACATTGCCGCTGCGGCCCAGTTGGGCGCGTGGTACCGCCTCGCGCTTGAGGcgcg                                                                                                                                              <  1:314141/91‑1 (MQ=255)                                                               ccAGGGCGTTTGAGCCAACCCAGCCAGCCCAGGGCGACATTGCCGCTGCGGCCCAGTTGGGCGCGTGGTACCGCCTCGCGCTTGAGGcgcg                                                                                                                                              >  2:314141/1‑91 (MQ=255)                                                                cAGGGCGTTTGAGCTATCCCAGCCAGCCCAGGGCGACATTGCCGCTGCGGCCCAGTTGGGCGCGTGGTACCGCCTCGCGCTTGAGGCGCGGGGCAATTTCGAAATCCAGTTCGGCACCTATATAGAAGCGCACCAACTCGACCAGCTTg                                                                                   <  1:117505/149‑1 (MQ=255)                                                                   ggCGTTTGAGCCAACCCAGCCAGCCCAGGGCGACATTGCCGCTGCGGCCCAGTTGGGCGCGTGGTACCGCCTCGCGCTTGAGGCGCGGGGCAATTTCGAAATCCAGTTCGGCACCTATATAGAAGCGCACCAACTCGACCAGCTTGCgg                                                                                <  6:333373/149‑1 (MQ=255)                                                                   ggCGTTTGAGCCAACCCAGCCAGCCCAGGGCGACATTGCCGCTGCGGCCCAGTTGGGCGCGTGGTACCGCCTCGCGCTTGAGGCGCGGGGCAATTTCGAAATCCAGTTCGGCACCTATATAGAAGCGCACCAACTCGACCAGCTTGCgg                                                                                <  5:106414/149‑1 (MQ=255)                                                                     cGTTTGAGCCAACCCAGCCAGCCCAGGGCGACATTGCCGCTGCGGCCCAGTTGGGCGCGTGGTACCGCCTCGCGCTTGAGGCGCGGGGCAATTTCGAAATCCAGTTCGGCACCTATATAGAAGCGCACCAACTCGACCAGCTTGCGGTa                                                                              <  2:112199/149‑1 (MQ=255)                                                                            gCCAACCCAGCCAGCCCAGGGCGACATTGCCGCTGCGGCCCAGTTGGGCGCGTGGTACCGCCTCGCGCTTGAGGCGCGGGGCAATTTCGAAATCCAGTTCGGCACCTATATAGAAGCGCACCAACTCGACCAGCTTGCGGTAGCAGGCg                                                                       >  5:200409/1‑149 (MQ=255)                                                                             ccAACCCAGCCAGCCCAGGGCGACATTGCCGCTGCGGCCCAGTTGGGCGCGTGGTACCGCCTCGCGCTTGAGGCGCGGGGCAATTTCGAAATCCAGTTCGGCACATATATAGAAGCGCACAAACTCGACCAGCTTGCGGTAGCAGGCgt                                                                      >  6:324293/1‑148 (MQ=255)                                                                              cAACCCAGCCAGCCCAGGGCGACATTGCCGCTGCGGCCCAGTTGGGCGCGTGGTACCGCCTCGCGCTTGAGGCGCGGGGCAATTTCGAAATCCAGTTCGGCACCTATATAGAAGCGCACCAACTCGACCAGCTTGCGGTAGCAGGCGGt                                                                     <  2:81732/149‑1 (MQ=255)                                                                              cAACCCAGCCAGCCCAGGGCGACATTGCCGCTGCGGCCCAGTTGGGCGCGTGGTACCGCCTAGCCCTTCAGCCCCGGGGCAAATTCGAAATACAGATCGGCACCTATATAGAAGCGCACCAACTCGACCAGCTAGCGGTAGCAGCCGGt                                                                     >  8:58383/1‑149 (MQ=255)                                                                                aCCCAGCCAGCCCAGGGCGACATTGCCGCTGCGGCCCAGTTGGGCGCGTGGTACCGCCTCGCGCTTGAGGCGCGGGGCAATTTCGAAATCCAGTTCGGCACCTATATAGAAGCGCACCAACTCGACCAGCTTGCGGTAGCAGGCGGTGc                                                                   >  5:273640/1‑149 (MQ=255)                                                                                  ccagccagCCCAGGGCGACATTGCCGCTGCGGCCCAGTTGGGCGCGTGGTACCGCCTCGCGCTTGAGGCGCGGGGCAATTTCGAAATCCAGTTCGGCACCTATATAGAAGCGCACCAACTCGACCAGCTTGCGGTAGCAGGCGGTGccc                                                                 >  7:136696/1‑149 (MQ=255)                                                                                        agccCAGGGCGACATTGCCGCTGCGGCCCAGTTGGGCGCGTGGTACCGCCTCGCGCTTGAGGCGCGGGGCAATTTCGAAATCCAGTTCGGCACCTATATAGAAGCGCACCAACTCGACCAGCTTGCGGTAGCAGGCGGTGCCCGGATg                                                            >  3:284268/1‑148 (MQ=255)                                                                                          cccAGGGCGACATTGCCGCTGCGGCCCAGTTGGGCGCGTGGTACCGCCTCGCGCTTGAGGCGCGGGGCAATTTCGAAATCCAGTTCGGCACCTATATAGAAGCGCACCAACTCGACCAGCTTGCGGTAGCAGGCGGTGCCCGGCTGGaa                                                         <  8:136696/149‑1 (MQ=255)                                                                                                 cGACATTGCCGCTGCGGCCCAGTTGGGCGCGTGGTACCGCCTCGCGCTTGAGGCGCGGGGCAATTTCGAAATCCAGTTCGGCACCTATATAGAAGCGCACCAACTCGACCAGCTTGCGGTAGCAGGCGGTGCCCGGCTGGAAGCGCTgg                                                  <  6:90215/149‑1 (MQ=255)                                                                                                 cGACATTGCCGCTGCGGCCCAGTTGGGCGCGTGGTACCGCCTCGCGCTTGAGGCGCGGGGCAATTTCGAAATCCAGTTCGGCACCTATATAGAAGCGCACCAACTCGACCAGCTTGCGGTAGCAGGCGGTGCCCGGCTGGAAGCGCTgg                                                  <  7:64694/149‑1 (MQ=255)                                                                                                         ccGCTGCGGCCCAGGTGGGCGCGTGGTACCGCCTCGCGCTTGAGGCGCGGGGCAATTTCGAAATCCAGTTCGGCACCTATATAGAAGCGCACCAACTCGACCAGCTTGCGGTAGCAGGCGGTGCCCGGCTGGAAGCGCTGGTAGTCGGc                                          <  8:216303/149‑1 (MQ=255)                                                                                                           gCTGCGGCCCAGTTGGGCGCGTGGTACCGCCTCGCGCTTGAGGCGCGGGGCAATTTCGAAATCCAGTTCGGCACCTATATAGAAGCGCACCAACTCGACCAGCTTGCGGTTGCAGTCTGTGCCAGGCTTGAAGCGCTGGTAGTCGGCCa                                        <  5:76227/149‑1 (MQ=255)                                                                                                               cGGCCCAGTTGGGCGCGTGGTACCGCCTCGCGCTTGAGGCGCGGGGCAATTTCGAAATCCAGTTCGGCACCTATATAGAAGCGCACCAACTCGACCAGCTTGCGGTAGCAGGCGGTGCCCGGCTGGAAGCGCTGGTAGTCGGCCAGGc                                     <  1:4909/148‑1 (MQ=255)                                                                                                                      gTTGGGCGCGTGGTACCGCCTCGCGCTTGAGGCGCGGGGCAATTTCGAAATCCAGTTCGGCACCTATATAGAAGCGCACCAACTCGACCAGc                                                                                      >  2:86768/1‑92 (MQ=255)                                                                                                                      gTTGGGCGCGTGGTACCGCCTCGCGCTTGAGGCGCGGGGCAATTTCGAAATCCAGTTCGGCACCTATATAGAAGCGCACCAACTCGACCAGc                                                                                      <  1:86768/92‑1 (MQ=255)                                                                                                                              cgTGGTACCGCCTCGCGCTTGAGGCGCGGGGCAATTTCGAAATCCAGTTCGGCACCTATATAGAAGCGCACCAACTCGACCAGCTTGCGGTAGCAGGCGGTGCCCGGCTg                                                            <  5:189588/110‑1 (MQ=255)                                                                                                                              cgTGGTACCGCCTCGCGCTTGAGGCGCGGGGCAATTTCGAAATCCAGTTCGGCACCTATATAGAAGCGCACCAACTCGACCAGCTTGCGGTAGCAGGCGGTGCCCGGCTg                                                            >  6:189588/1‑110 (MQ=255)                                                                                                                                 gaaattgCCCCGCGCCTCAAGCGCGGGGCAATTTCGAAATCCAGTTCGGCACCTATATAGAAGCGCACCAACTCGACCAGCTTGCGGTAGCAGGCGGTGCCCGGTTGGAAGCGCTGGTAGTCGGCCAGGCTGAGCGGGCCCAGCTCGAt                  >  2:55792/7‑149 (MQ=255)                                                                                                                                 gaaattgCCCCGCGCCTCAAGCGCGGGGCAATTTCGAAATCCAGTTCGGCACCTATATAGAAGCGCACCAACTCGACCAGCTTGCGGTAGCAGGCGGTGCCCGGTTGGAAGCGCTGGTAGTCGGCCAGGCTGAGCGGGCCCAGCTCGAt                  >  4:245168/7‑149 (MQ=255)                                                                                                                                   tACCGCCTCGCGCTTGAGGCGCGGGGCAATTTCGAAATCCAGTTCGGCACCTATATAGAAGCGCACCAACTCGACCAGCTTGCGGTAGCAGGCGGTGCCCGGCTGGAAGCGCTGGTAGTCGGCCAGGCTGAGCGGGCCCAGCTCGATGc                >  6:309484/1‑149 (MQ=255)                                                                                                                                   tACCGCCTCGCGCTTGAGGCGCGGGGCAATTTCGAAATCCAGTTCGGCACCTATATAGAAGCGCACCAACTCGACCAGCTTGCGGTAGCAGGCGGTGCCCGGCTGGAAGCGCTGGTAGTCGGCCAGGCTGAGCGGGCCCAGCTCGATGc                <  1:340404/149‑1 (MQ=255)                                                                                                                                       gCCCCGCGCCTCAAGCGCGGGGCAATTTCGAAATCCAGTTCGGCACCTATATAGAAGCGCACCAACTCGACCAGCTTGCGGTAGCAGGCGGTGCCCGGTTGGAAGCGCTGGTAGTCGGCCAGGCTGAGCGGGCCCAGCTCGATGCGGa             <  3:245168/148‑1 (MQ=255)                                                                                                                                       gCCCCGCGCCTCAAGCGCGGGGCAATTTCGAAATCCAGTTCGGCACCTATATAGAAGCGCACCAACTCGACCAGCTTGCGGTAGCAGGCGGTGCCCGGTTGGAAGCGCTGGTAGTCGGCCAGGCTGAGCGGGCCCAGCTCGATGCGGa             <  1:55792/148‑1 (MQ=255)                                                                                                                                        ccccgcgCCTCAAGCGCGGGGCAATTTCGAAATCCAGTTCGGCACCTATATAGAAGCGCACCAACTCGACCAGCTTGCGGTAGCAGGCGGTGCCCGGCTGGAAGCCCTGGTAGTCGGCCAGGCTGAGCGGGCCCAGCTCGATGCGGATg           >  1:13000/4‑149 (MQ=255)                                                                                                                                              gcTTGAGGCGCGGGGCAATTTCGAAATCCAGTTCGGCACCTATATAGAAGCGCACCAACTCGACCAGCTTGCGGTAGCAGGCGGTGCCCGGCTGGAAGCGCTGGTAGTCGGCCAGGCTGAGCGGGCCCAGCTCGATGCGGATGCACGAc     <  5:324293/149‑1 (MQ=255)                                                                                                                                                  gAGGCGCGGGTCAATTTCGAAATCCAGTTCGGCACCTATATAGAAGCGCACCAACTCGACCAGCTTGCGGTAGCAGGCGGTGCCCGGCTGGAAGCGCTGGTAGTCGGCCAGGCTGAGCGGGCCCAGCTCGATGCGGATGCACGACTgg  <  8:204935/148‑1 (MQ=255)                                                                                                                                                  |                                                                                                                                                    ACAAGGCGACCCCATCGTAAGGAATGTGGAAGACGGCGCAACGCGAAGGCTCCGCGTCACGACCAGGGCGTTTGAGCCAACCCAGCCAGCCCAGGGCGACATTGCCGCTGCGGCCCAGTTGGGCGCGTGGTACCGCCTCGCGCTTGAGGCGCGGGGCAATTTCGAAATCCAGTTCGGCACCTATATAGAAGCGCACCAACTCGACCAGCTTGCGGTAGCAGGCGGTGCCCGGCTGGAAGCGCTGGTAGTCGGCCAGGCTGAGCGGGCCCAGCTCGATGCGGATGCACGACTGG  >  NC_002947/3491350‑3491642

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑