Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,570,543 T→C 6.8% M108V (ATG→GTG)  sad‑II ← NAD+‑dependent succinate semialdehyde dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,570,5430TC 6.8% 210.5 / 4.0 73M108V (ATG→GTG) sad‑IINAD+‑dependent succinate semialdehyde dehydrogenase
Reads supporting (aligned to +/- strand):  ref base T (28/40);  new base C (2/3);  total (30/43)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.30e-01

ACGTAGGTGTTACCCGCCAGAATCGCCGGCACGGCGCCGCGCAGCACCTGCCAGACCGGGAAGTTCCACGGCATCACGGCCAGGATCGGGCCCAGCGGGCGGTATTCGATACGGGCTTTTTCGACCTGGGTCGGCTCGGCGGCGAGCATGGCCGGGCCGTGCTCGGCGTACCAGCGGCAAAGGCCTACACACTTGCTGACTTCACCGCGAGCCTGGGCGATCGGCTTGCCAATTTCGCGGCTGATCATGTGCGCGAAAGCTTCAGCCTTGGCTTCGAGGGTGTCGGCCAGGGCCAG  >  NC_002947/3570395‑3570690
                                                                                                                                                    |                                                                                                                                                   
aCGTAGGTGTTACCCGCCAGAATCGCCGGCACGGCGCCGCGCAGCACCTGCCAGACCGGGAAGTTCCACGGCATCACGGCCAGGATCGGGCCCAGCGGGCGGTATTCGATACGGGCTTTTTCGACCTGGGTCGGCTCGGCGGCGAGCAt                                                                                                                                                     >  4:311550/1‑149 (MQ=255)
    aGGTGTTACCCGCCAGAATCGCCGGCACGGCGCCGCGCAGCACCTGCCAGACCGGGAAGTTCCACGGCATCACGGCCAGGATCGGGCCCAGCGGGCGGTATTCGATACGGGCTTTTTCGACCTGGGTCGGCTCGGCGGCGAGCATggcc                                                                                                                                                 >  2:52774/1‑149 (MQ=255)
     ggTGTTACCCGCCAGAATCGCCGGCACGGCGCCGCGCAGCACCTGCCAGACCGGGAAGTTCCACGGCATCACGGCCAGGATCGGGCCCAGCGGGCGGTATTCGATACGGGCTTTTTCGACCTGGGTCGGCTCGGCGGCGAGCATggccg                                                                                                                                                >  8:249269/1‑149 (MQ=255)
         ttACCCGCCAGAATCGCCGGCACGGCGCCGCGCAGCACCTGCCAGACCGGGAAGTTCCCCGGCATCACGGCCAGGATCGGGCCCAGCGGGCGGTATTCGATACGGGCTTTTTCGACCTGGGTCGGCTCGGCGGCGAGCATggccgggcc                                                                                                                                            >  6:157234/1‑149 (MQ=255)
             ccGCCAGAATCGCCGGCACGGCGCCGCGCAGCACCTGCCAGACCGGGAAGTTCCACGGCATCACGGCCAGGATCGGGCCCAGCGGGCGGTATTCGATACGGGCTTTTTCGACCTGGGTCGGCTCGGCGGCGAGCATGGCCGGGCCGTGc                                                                                                                                        >  2:19893/1‑149 (MQ=255)
              cGCCAGAATCGCCGGCACGGCGCCGCGCAGCACCTGCCAGACCGGGAAGTTCCACGGCATCACGGCCAGGATCGGGCCCAGCGGGCGGTATTCGATACGGGCTTTTTCGACCTGGGTCGGCTCGGCGGCGAGCATGGCCGGGCCGTGCt                                                                                                                                       >  2:13788/1‑149 (MQ=255)
               gCCAGAATCGCCGGCACGGCGCCGCGCAGCACCTGCCAGACCGGGAAGTTCCACGGCATCACGGCCAGGATCGGGCCCAGCGGGCGGTATTCGCTACGGGCTTTTTCGACCTGGGTCGGCTCGGCGGCGAGCATGGCCGGGCCGTGCTc                                                                                                                                      >  1:196399/1‑149 (MQ=255)
               gCCAGAATCGCCGGCACGGCGCCGCGCAGCACCTGCCAGACCGGGAAGTTCCACGGCATCACGGCCAGGATCGGGCCCAGCGGGCGGTATTCGATACGGGCTTTTTCGACCTGGGTCGGCTCGGCGGCGAGCACGGCCCGGCCATGCTc                                                                                                                                      <  8:300365/149‑1 (MQ=255)
               gCCAGAATCGCCGGCACGGCGCCGCGCAGCACCTGCCAGACCGGGAAGTTCCACGGCATCACGGCCAGGATCGGGCCCAGCGGGCGGTATTCGATACGGGCTTTTTCGACCTGGGTCGGCTCGGCGGCGAGCACGGCCCGGCCATGCTc                                                                                                                                      <  5:84809/149‑1 (MQ=255)
               gCCAGAATCGCCGGCACGGCGCCGCGCAGCACCTGCCAGACCGGGAAGTTCCACGGCATCACGGCCAGGATCGGGCCCAGCGGGCGGTATTCGATACGGGCTTTTTCGACCTGGGTCGGCTCGGCGGCGAGCACGGCCCGGCCATGCTc                                                                                                                                      >  6:84809/1‑149 (MQ=255)
                ccAGAATCGCCGGCACGGCGCCGCGCAGCACCTGCCAGACCGGGAAGTTCCACGGCATCACGGCCAGGATCGGGCCCAGCGGGCGGTATTCGATACGGGCTTTTTCGACCTGGGTCGGCTCGGCGGCGAGCATGGCCGGGCCGTGCTCg                                                                                                                                     <  3:62812/149‑1 (MQ=255)
                ccAGAATCGCCGGCACGGCGCCGCGCAGCACCTGCCAGACCGGGAAGTTCCACGGCATCACGGCCAGGATCGGGCCCAGCGGGCGGTATTCGATACGGGCTTCTTCGACCTGGGTCGGCTCGGCGGCGAGCATGGCCGGGCCGTGCTCg                                                                                                                                     <  1:245637/149‑1 (MQ=255)
                 cAGAATCGCCGGCACGGCGCCGCGCAGCACCTGCCAGACCGGGAAGTTCCACGGCATCACGGCCAGGATCGGGCCCAGCGGGCGGTATTCGATACGGGCTTTTTCGACCTGGGTCGGCTCGGCGGCGAGCATGGCCGGGCCGTGCTCgg                                                                                                                                    <  2:262784/149‑1 (MQ=255)
                   gAATCGCCGGCACGGCGCCGCGCAGCACCTGCCAGACCGGGAAGTTCCACGGCATCACGGCCCGGATCGGGCCCCGCGGGCGGTATTCGATACGGGCTTTTTCGACCTGGGTCGGCTCGGCGGCGAGCATGGCCGGGCCGTGCTCGGCg                                                                                                                                  >  3:89028/1‑149 (MQ=255)
                     aTCGCCGGCACGGCGCCGCGCAGCACCTGCCAGACCGGGAAGTTCCACGGCATCACGGCCAGGATCGGGCCCAGCGGGCGGTATTCGATACGGGCTTTTTCGACCTGGGTCGGCTCGGCGGCGAGCATGGCCGGGCCGTGCTCGGCGt                                                                                                                                 <  4:128192/148‑1 (MQ=255)
                      tCGCCGGCACGGCGCCGCGCAGCACCTGCCAGACCGGGAAGTTCCACGGCATCACGGCCAGGATCGGGCCCAGCGGGCGGTATTCGATACGGGCTTTTTCGACCTGGGTCGGCTCGGCGGCGAGCATGGCCGGGCCGTGCTCGGCGTAc                                                                                                                               <  3:242142/149‑1 (MQ=255)
                             cACGGCGCCGCGCAGCACCTGCCAGACCGGGAAGTTCCACGGCATCACGGCCAGGATCGGGCCCAGCGGGCGGTATTCGATACGGGCTTTTTCGACCTGGGTCGGCTCGGCGGCGAGCATggccggg                                                                                                                                              <  3:62403/127‑1 (MQ=255)
                             cACGGCGCCGCGCAGCACCTGCCAGACCGGGAAGTTCCACGGCATCACGGCCAGGATCGGGCCCAGCGGGCGGTATTCGATACGGGCTTTTTCGACCTGGGTCGGCTCGGCGGCGAGCATggccggg                                                                                                                                              >  4:62403/1‑127 (MQ=255)
                              aCGGCGCCGCGCAGCACCTGCCAGACCGGGAAGTTCCACGGCATCACGGCCAGGATCGGGCCCAGCGGGCGGTATTCGATACGGGCTTTTTCGACCTGGGTCGGCTCGGCGGCGAGCATGGCCGGGCCGTGCTCGGCGTACCAGCGGc                                                                                                                        >  1:52566/1‑148 (MQ=255)
                                 gcgcCGCGCAGCACCTGCCAGACCGGGAAGTTCCACGGCATCACGGCCAGGATCGGGCCCAGCGGGCGGTATTCGATACGGGCTTTTTCGACCTGGGTCGGCTCGGCGGCGAGCATGGCCGGGCCGTGCTCGGCGTACCAGCGGCAAAg                                                                                                                    <  7:249269/149‑1 (MQ=255)
                                       cgcAGCACCTGCCAGACCGGGAAGTTCCACGGCATCACGGCCAGGATCGGGCCCAGCGGGCGGTATTCGATACGGGCTTTTTCGACCTGGGTCGGCTCGGCGGCGAGCATGGCCGGGCCGTGCTGGGCGTACCAGCGGCAAAGGCCTac                                                                                                              <  7:48727/149‑1 (MQ=255)
                                           gcaCCTGCCAGACCGGGAAGTTCCACGGCATCACGGCCAGGATCGGGCCCAGCGGGCGGTATTCGATACGGGCTTTTTCGACCTGGGTCGGCTCGGCGGCGAGCATGGCCGGGCCGTGCTCGGCGTACCAGCGGCAAAGGCCTacacac                                                                                                          <  2:142675/149‑1 (MQ=255)
                                               cTGCCAGACCGGGAAGTTCCACGGCATCACGGCCAGGATCGGGCCCAGCGGGCGGTATTCGATACGGGCTTTTTCGACCTGGGTCGGCTCGGCGGCGAGCATGGCCGGGCCGTGCTCGGCGTACCAGCGGCAAAGGCCTACACACTTGc                                                                                                      <  2:52566/149‑1 (MQ=255)
                                                  ccAGACCGGGAAGTTCCACGGCATCACGGCCAGGATCGGGCCCAGCGGGCGGTATTCGATACGGGCTTTTTCGACCTGGGTCGGCTCGGCGGCGAGCATGGCCGGGCCGTGCTCGGCGTACCAGCGGCAAAGGCCTACACACTTGCTGa                                                                                                   <  3:311550/149‑1 (MQ=255)
                                                  ccAGACCGGGAAGTTCCACGGCATCACGGCCAGGATCGGGCCCAGCGGGCGGTATTCGATACGGGCTTTTTCGACCTGGGTCGGCTCGGCGGCGAGCATGGCCGGGCCGTGCTCGGCGTACCAGCGGCAAAGGCCTACACACTTGCTGa                                                                                                   <  6:49926/149‑1 (MQ=255)
                                                  ccAGACCGGGAAGTTCCACGGCATCACGGCCAGGATCGGGCCCAGCGGGCGGTATTCGATACGGGCTTTTTCGACCTGGGGCGGCTCGGCGGCGAGCATGGCCGGGCCGTGCTCGGCGTACCAGCGGCAAAGGCCTACACACTTGCt                                                                                                     <  1:266503/147‑1 (MQ=255)
                                                       ccGGGAAGTTCCACGGCATCACGGCCAGGATCGGGCCCAGCGGGCGGTATTCGATACGGGCTTTTTCGACCTGGGTCGGCTCGGCGGCGAGCATGGCCGGGCCGTGCTCGGCGTACCAGCGGCAAAGGCCTACACACTTGCTGACTTCa                                                                                              <  1:13788/149‑1 (MQ=255)
                                                        cGGGAAGTTCCACGGCATCACGGCCAGGATCGGGCCCAGCGGGCGGTATTCGATACGGGCTTTTTCGACCTGGGTCGGCTCGGCGGCGAGCATGGCCGGGCCGTGCTCGGCGTACCAGCGGCAAAGGCCTACACACTTGCTGACTTctc                                                                                             >  1:321787/1‑147 (MQ=255)
                                                        cGGGAAGTTCCACGGCATCACGGCCAGGATCGGGCCCAGCGGGCGGTATTCGATACGGGCTTTTTCGACCTGGGTCGGCTCGGCGGCGAGCATGGCCGGGCCGTGCTCGGCGTACCAGCGGCAAAGGCCTACACACTTGCTGACTTCAc                                                                                             >  8:250461/1‑149 (MQ=255)
                                                           gAAGTTCCACGGCATCACGGCCAGGATCGGGCCCAGCGGGCGGTATTCGATACGGGCTTTTTCGACCTGGGTCGGCTCGGCGGCGAGCATGGCCGGGCCGTGCTCGGCGTACCAGCGGCAAAGGCCTACACACTTGCTGACTTCACcgc                                                                                          <  1:19893/149‑1 (MQ=255)
                                                             aGTTCCACGGCATCACGGCCAGGATCGGGCCCAGCGGGCGGTATTCGATACGGTCTTTTTCGACCTGGGTCGGCTCGGCGGCGAGCATGGCCGGGCCGTGCTCGGCGTACCAGCGGCAAAGGCCTACACACTTGCTGACTTCACCGCGa                                                                                        <  2:215836/149‑1 (MQ=255)
                                                               ttCCACGGCATCACGGCCAGGATCGGGCCCAGCGGGCGGTATTCGATACGGGCTTTTTCGACCTGGGTCGGCTCGGCGGCGAGCATGGCCGGGCCGTGCTCGGCGTACCAGCGGCAAAGGCCTACACACTTGCTGACTTCACCGCGAGc                                                                                      <  8:172535/149‑1 (MQ=255)
                                                                 ccACGGCATCACGGCCAGGATCGGGCCCAGCGGGCGGTATTCGATACGGGCTTTTTCGACCTGGGTCGGCTCGGCGGCGAGCATGGCCGGGCCGTGCTCGGCGTACCAGCGGCAAAGGCCTacacac                                                                                                          <  7:69327/127‑1 (MQ=255)
                                                                 ccACGGCATCACGGCCAGGATCGGGCCCAGCGGGCGGTATTCGATACGGGCTTTTTCGACCTGGGTCGGCTCGGCGGCGAGCATGGCCGGGCCGTGCTCGGCGTACCAGCGGCAAAGGCCTacacac                                                                                                          >  8:69327/1‑127 (MQ=255)
                                                                  cACGGCATCACGGCCAGGATCGGGCCCAGCGGGCGGTATTCGATACGGGCTTTTTCGACCTGGGTCGGCTCGGCGGCGAGCATGGCCGGGCCGTGCTCGGCGTACCAGCGGCAAAGGCCTACACACTTGCTGACTTCACCGCGAGCCTg                                                                                   >  6:166105/1‑149 (MQ=255)
                                                                  cACGGCATCACGGCCAGGATCGGGCCCAGCGGGCGGTATTCGATACGGGCTTTTTCGACCTGGGTCGGCTCGGCGGCGAGCATGGCCGGGCCGTGCTCGGCGTACCAGCGGCAAAGGCCTACACACTTGCTGACTTCACCGCGAGCCTg                                                                                   >  4:302443/1‑149 (MQ=255)
                                                                   aCGGCATCACGGCTAGGATCGGGCCCAGCGGGCGGTATTCGATACGGGCTTTTTCGACCTGGGTCGGCTCGGCGGCGAGCATGGCCGGGCCGTGCTCGGCGTACCAGCGGCAAAGGCCTACACACTTGCTGACTTCACCGCGAGCCTgg                                                                                  >  5:69788/1‑149 (MQ=255)
                                                                     ggCATCACGGCCAGGATCGGGCCCAGCGGGCGGTATTCGATACGGGCTTTTTCGACCTGGGTCGGCTCGGCGGCGAGCATGGCCGGGCCGTGCTCGGCGTACCAGCGGCAAAGGCCTACACACTTGCTGACTTCACCGCGAGCCTGGGc                                                                                <  1:135597/149‑1 (MQ=255)
                                                                              gCCAGGATCGGGCCCAGCGGGCGGTATTCGATACGGGCTTTTTCGACCTGGGTCGGCTCGGCGGCGAGCATGGCCGGGTCGTGCTCGGCGTACCAGCGGCAAAGGCCTACACACTTGCTGACTTCACCGCGAGCCTGGGCGATCGGCtt                                                                       <  3:222623/149‑1 (MQ=255)
                                                                               ccAGGATCGGGCCCAGCGGGCGGTATTCGATACGGGCTTTTTCGACCTGGGTCGGCTCGGCGGCGAGCATGGCCGGGCCGTGCTCGGCGTACCAGCGGCAAAGGCCTACACACTTGCTGACTTCTCCGCGAGCCTGGGCGATCGGCTTg                                                                      <  2:321787/149‑1 (MQ=255)
                                                                                cAGGATCGGGCCCAGCGGGCGGTATTCGATACGGGCTTTTTCGACCTGGGTCGGCTCGGCGGCGAGCATGGCCGGGCCGTGCTCGGCGTACCAGCGGCAAAGGCCTACACACTTGCTGACTTCACCGCGAGCCTGGGCGATCGGCTTGc                                                                     <  5:84321/149‑1 (MQ=255)
                                                                                cAGGATCGGGCCCAGCGGGCGGTATTCGATACGGGCTTTTTCGACCTGGGTCGGCTCGGCGGCGAGCATGGCCGGGCCGTGCTCGGCGTACCAGCGGCAAAGGCCTACACACTTGCTGACTTCACCGCGAGCCTGGGCGATCGGCTTGc                                                                     >  3:152232/1‑149 (MQ=255)
                                                                                          cccAGCGGGCGGTATTCGATACGGGCTTTTTCGACCTGGGTCGGCTCGGCGGCGAGCATGGCCGGGCCGTGCTCGGCGTACCAGCGGCAAAGGCCTACACACTTGCTGACTTCACCGCGAGCCTGGGCGATCGGCTTGCCAATTTcgcg                                                           <  7:250461/149‑1 (MQ=255)
                                                                                          cccAGCGGGCGGTATTCGATACGGGCTTTTTCGACCTGGGTCGGCTCGGCGGCGAGCATGGCCGGGCCGTGCTCGGCGTACCAGCGGCAAAGGCCTACACACTTGCTGACTTCACCGCGAGCCTGGGCGATCGGCTTGCCAATTTcgcg                                                           <  3:302443/149‑1 (MQ=255)
                                                                                             aGCGGGCGGTATTCGATACGGGCTTTTTCGACCTGGGTCGGCTCGGCGGCGAGCATGGCCGGGCCGTGCTCGGCGTACCAGCGGCAAAGGCCTACACACTTGCTGACTTCACCGCGAGCCTGGGCGATCGGCTTGACAATTTCGCGGCt                                                        >  3:172634/1‑149 (MQ=255)
                                                                                                 ggcggTATTCGATACGGGCTTTTTCGACCTGGGTCGGCTCGGCGGCGAGCATGGCCGGGCCGTGCTCGGCGTACCAGCGGCAAAGGCCTACACACTTGCTGACTTCACCGCGAGCCTGGGCGATCGGCTTGCCAATTTCGCGGCTGAt                                                     >  4:54210/1‑148 (MQ=255)
                                                                                                   cctgtggcGATACGGGCTTTTTCGACCTGGGTCGGCTCGGCGGCGAGCATGGCCGGGCCGTGCTCGGCGTACCAGCGGCAAAGGCCTACACACTTGCTGACTTCACCGCAAGCCTGGGCGATCGGCTTGCCAATTTCGCGGCTGATCAt                                                  <  8:29971/142‑1 (MQ=255)
                                                                                                    ggTATTCGATACGGGCTTTTTCGACCTGGGTCGGCTCGGCGGCGAGCATGGCCGGGCCGTGCTCGGCGTACCAGCGGCAAAGGCCTACACACTTGCTGACTTCACCGCGAGCCTGGGCGATCGGCtt                                                                       <  1:320040/127‑1 (MQ=255)
                                                                                                    ggTATTCGATACGGGCTTTTTCGACCTGGGTCGGCTCGGCGGCGAGCATGGCCGGGCCGTGCTCGGCGTACCAGCGGCAAAGGCCTACACACTTGCTGACTTCACCGCGAGCCTGGGCGATCGGCtt                                                                       >  2:320040/1‑127 (MQ=255)
                                                                                                        ttCGATACGGGCTTTTTCGACCTGGGTCGGCTCGGCGGCGAGCATGGCCGGGCCGTGCTCGGCGTACCAGCGGCAAAGGCCTACACACTTGCTGACTTCACCGCGAGCCTGGGCGATCGGCTTGCCAATTTCGCGGCTGATCATGTgc                                              <  4:89028/148‑1 (MQ=255)
                                                                                                           gATACGGGCTTTTTCGACCTGGGTCGGCTCGGCGGCGAGCATGGCCGGGCCGTGCTCGGCGTACCAGCGGCAAAGGCCTACACACTTGCTGACTTCACCGCGAGCCTGGGCGATCGGCTTGCCAATTTCGCGGCTGATCATGTGcgcg                                           >  7:179748/1‑148 (MQ=255)
                                                                                                           gATACGGGCTTTTTCGACCTGGGTCGGCTCGGCGGCGAGCATGGCCGGGCCGTGCTCGGCGTACCAGCGGCAAAGGCCTACACACTTGCTGACTTCACCGCGAGCCTGGGCGATCGGCTTGCCAATTTCGCGCCTGATCATGTGcgcg                                           >  7:247380/1‑148 (MQ=255)
                                                                                                             tACGGGCTTTTTCGACCTGGGTCGGCTCGGCGGCGAGCATGGCCGGGCCGTGCTCGGCGTACCAGCGGCAAAGGCCTACACACTTGCTGACTTCACCGCGAGCCTGGGCGATCGGCTTGCCAATTTCGCGGCTGATCATGTgcgc                                            <  8:227495/145‑1 (MQ=255)
                                                                                                             tACGGGCTTTTTCGACCTGGGTCGGCTCGGCGGCGAGCATGGCCGGGCCGTGCTCGGCGTACCAGCGGCAAAGGCCTACACACTTGCTGACTTCACCGCGAGCCTGGGCGATCGGCTTGCCAATTTCGCGGCTGATCATGTgcgc                                            >  7:227495/1‑145 (MQ=255)
                                                                                                             tACGGGCTTTTTCGACCTGGGTCGGCTCGGCGGCGAGCATGGCCGGGCCGTGCTCGGCGTACCAGCGGCAAAGGCCTACACACTTGCTGACTTCACCGCGAGCCTGGGCGATCGGCTTGCCAATTTCGCGGCTGATCATGTGCGCGaaa                                        <  4:16815/149‑1 (MQ=255)
                                                                                                                gggCTTTTTCGACCTGGGTCGGCTCGGCGGCGAGCATGGCCGGGCCGTGCTCGGCGTACCAGCGGCAAAGGCCTACACACTTGCTGACTTCACCGCGAGCCTGGGCGATCGGCTTGCCAATTTCGCGGCTGATCATGTGCGCGAAAGCt                                     >  5:267992/1‑149 (MQ=255)
                                                                                                                  gCTTTTTCGACCTGGGTCGGCTCGGCGGCGAGCATGGCCGGGCCGTGCTCGGCGTACCAGCGGCAAAGGCCTACACACTTGCTGACTTCACCGCGAGCCTGGGCGATCGGCTTGCCAATTTCGCGGCTGATCATGTGCGCGAAAGCTTc                                   <  4:172634/149‑1 (MQ=255)
                                                                                                                   cTTTTTCGACCTGGGTCGGCTCGGCGGCGAGCATGGCCGGGCCGTGCTCGGCGTACCAGCGGCAAAGGCCTACACACTTGCTGACTTCACCGCGAGCCTGGGCGATCGGCTTGCCAATTTCGCGGCTGATCATGTGCGCGAAAGCTTCa                                  <  7:210057/149‑1 (MQ=255)
                                                                                                                          gACCTGGGTCGGCTCGGCGGCGAGCATGGCCGGGCCGTGCTCGGCGTACCAGCGGCAAAGGCCTACACACTTGCTGACTTCACCGCGAGCCTGGGCGATCGGCTTGCCAATTTCGCGGCTGATCATGTGCGCGAAAGCTTCAGCCTTgg                           >  3:217761/1‑149 (MQ=255)
                                                                                                                          gACCTGGGTCGGCTCGGCGGCGAGCATGGCCGGGCCGTGCTCGGCGTACCAGCGGCAAAGGCCTACACACTTGCTGACTTCACCGCGAGCCTGGGCGATCGGCTTGCCAATTTCGCGGCTGATCATGTGCGCGAAAGCTTCAGCCTTg                            <  1:52774/148‑1 (MQ=255)
                                                                                                                          gACCTGGGTCGGCTCGGCGGCGAGCATGGCCGGGCCGTGCTCGGCGTACCAGCGCCAAAGGCCTACACACTTGCTGACTTCACCGCCAGCCTGGGCGTTCGGCTTGCCAATTTCGCGGCCGATCATGTGCGCGAAAGCTTCAGCCTTg                            <  1:20263/148‑1 (MQ=255)
                                                                                                                                ggTCGGCTGGGCGGCGAGCATGGCCGGGCCGTGCTCGGCGTACCAGCGGCAATGGCCTACACACTTGCTGAGTTCACCGCGAGCCTTGGCGATCGGCTTGCCAATTTCGCGGCTGATCATGTGCGCGAAAGCTTCAGCCTTGGCTTCGa                     <  5:157234/149‑1 (MQ=255)
                                                                                                                                ggTCGGCTCGGCGGCGAGCATGGCCGGGCCGTGCTCGGCGTACCAGCGGCAAAGGCCTACACACTTGCTGACTTCACCGCGAGCCTGGGCGATCGGCTTGCCAATTTCGCGGCTGATCATGTGCGCGAAAGCTTCAGCCTTGGCTTCGa                     <  4:152232/149‑1 (MQ=255)
                                                                                                                                 gTCGGCTCGGCGGCGAGGATGGCCGGGCCGTGCTCGGCGTACCAGCGGCAAAGGCCTACACACTTGCTGACTTCACCGCGAGCCTGGGCGATCGGCTTGCCAATTTCGCGGCTGATCATGTGCGCGAAAGCTTGAGCCTTGGCTTCGAg                    <  3:54210/149‑1 (MQ=255)
                                                                                                                                    ggctcggcGGCGAGCATGGCCGGGCCGTGCTCGGCGTACCAGCGGCAAAGGCCTACACACTTGCTGACTTCACCGCGAGCCTGGGCGATCGGCTTGCCAATTTCGCGGCTGATCATGTGCGCGAAAGCTTCAGCCTTGGCTTCGAGGgt                 <  6:267992/149‑1 (MQ=255)
                                                                                                                                      ctcggcGGCGAGCATGGCCGGGCCGTGCTCGGCGTACCAGCGGCAAAGGCCTACACACTTGCTGACTTCACCGCGAGCCTGGGCGATCGGCTTGCCAATTTCGCGGCTGATCATGTGCGCGAAAGCTTCAGCCtt                             <  2:195874/135‑1 (MQ=255)
                                                                                                                                      ctcggcGGCGAGCATGGCCGGGCCGTGCTCGGCGTACCAGCGGCAAAGGCCTACACACTTGCTGACTTCACCGCGAGCCTGGGCGATCGGCTTGCCAATTTCGCGGCTGATCATGTGCGCGAAAGCTTCAGCCtt                             >  1:195874/1‑135 (MQ=255)
                                                                                                                                         ggcggcGAGCATGGCCGGGCCGTGCTCGGCGTACCAGCGGCAAAGGCCTACACACTTGCTGACTTCACCGCGAGCCTGGGCGATCGGCTTGCCAATTTCGCGGCTGATCATGTGCGCGAAAGCTTCAGCCTTGGCTTCGAGGGTGTCg             <  4:119003/148‑1 (MQ=255)
                                                                                                                                              cgAGCACGGCCCGGCCATGCTCGGCGTACCAGCGGCAAAGGCCTACACACTTGCTGACTTCACCGCGAGCCTGGGCGATCGGCTTGCCAATTTCGCGGCTGATCATGTGCGCGAAAGCTTCAGCCTTGGCTTCGAGGGTGTCggccagg       >  6:266561/1‑149 (MQ=255)
                                                                                                                                                aGCACGGCCCGGCCATGCTCGGCGTACCAGCGGCAAAGGCCTACACACTTGCTGACTTCACCGCGAGCCTGGGCGATCGGCTTGCCAATTTCGCGGCTGATCATGTGCGCGAAAGCTTCAGCCTTGGCTTCGAGGGTGTCggccagggc     <  5:266561/149‑1 (MQ=255)
                                                                                                                                                 gCATGGCCGGGCCGTGCTCGGCGTACCAGCGGCAAAGGCCTACACACTTGCTGACTTCACCGCGAGCCTGGGCGATCGGCTTGCCAATTTCGCGGCTGATCATGTGCGCGAAAGCTTCAGCCTTGGCTTCGAGGGTGTCggccagggcc    >  8:223550/1‑149 (MQ=255)
                                                                                                                                                 gCATGGCCGGGCCGTGCTCGGCGTACCAGCGGCAAAGGCCTACACACTTGCTGACTTCACAGCGAGCCTGGGCGATCGGCTTGCCAATTTCGCGGCTGATCATGTGCGCGAAAGCTTCAGCCTTGGCTTCGAGGGTGTCggccagggc     >  4:247198/1‑148 (MQ=255)
                                                                                                                                                   aTGGCCGGGCCGTGCTCGGCGTACCAGCGGCAAAGGCCTACACACTTGCTGACTTCACCGCGAGCCTGGGCGATCGGCTTGCCAATTTCGCGGCTGATCATGTGCGCGAAAGCTTCAGCCTTGGCTTCGAGGGTGTCggccagggccag  <  6:69788/149‑1 (MQ=255)
                                                                                                                                                   aTGGCCGGGCCGTGCTCGGCGTACCAGCGGCAAAGGCCTACACACTTGCTGACTTCACCGCGAGCCTGGGCGATCGGCTTGCCAATTTCGCGGCTGATCATGTGCGCGAAAGCTTCAGCCTTGGCTTCGAGGGTGTCggccagggccag  >  3:29477/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
ACGTAGGTGTTACCCGCCAGAATCGCCGGCACGGCGCCGCGCAGCACCTGCCAGACCGGGAAGTTCCACGGCATCACGGCCAGGATCGGGCCCAGCGGGCGGTATTCGATACGGGCTTTTTCGACCTGGGTCGGCTCGGCGGCGAGCATGGCCGGGCCGTGCTCGGCGTACCAGCGGCAAAGGCCTACACACTTGCTGACTTCACCGCGAGCCTGGGCGATCGGCTTGCCAATTTCGCGGCTGATCATGTGCGCGAAAGCTTCAGCCTTGGCTTCGAGGGTGTCGGCCAGGGCCAG  >  NC_002947/3570395‑3570690

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑