Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,630,322 T→C 6.6% W315R (TGG→CGG)  PP_3200 → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,630,3220TC 6.6% 172.7 / 2.2 60W315R (TGG→CGG) PP_3200hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (27/29);  new base C (2/2);  total (29/31)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.18e-01

CTATTTCTCCACAGACGACTTCTTTGGCGATGGCGCCGAGCGCATGTTCGTCGGCGCGCCGGTGTTCCCCAGTGGCCAACCTCTG‑GCGTTCTTCCACGGCAACGACAAGCAAGCCAAGGACTCAGGGCAGGGCGGCATGCAGGTGCGCTGGCGCACCGAGACGGTCGACTGGGGCCTGTACGCCATCCGTTTCCACGACAAGTCGCCGCAACTCAATGTACGCCCGAACTTCGCCAACCTCGACCCGGTCAGCGGCAAGGCCGGCGAGTTCTACTGGGTGTATCCCGAGGGTATC  >  NC_002947/3630174‑3630468
                                                                                                                                                     |                                                                                                                                                  
cTATTTCTCCACAGACGACTTCTTTGGCGATGGCGCCGAGCGCATGTTCGTCGGCGCGCCGGTGTTCCCCAGTGGCCAACCTCTG‑GCGTTCTTCCACGGCATCGACAAGCAAGCCAAGGACTCAGGGCAGGGCGGCATGCAGGTGCGCt                                                                                                                                                    <  8:192974/149‑1 (MQ=255)
  aTTTCTCCACAGACGACTTCTTTGGCGATGGCGCCGAGCGCATGTTCGTCGGCGCGCCGGTGTTCCCCAGTGGCCAACCTCTG‑GCGTTCTTCCACGGCAACGACAAGCAAGCCAAGGACTCAGGGCAGGGCGGCATGCAGGTGCGCTgg                                                                                                                                                  >  4:252026/1‑149 (MQ=255)
   tttCTCCACAGACGACTTCTTTGGCGATGGCGCCGAGCGCATGTTCGTCGGCGCGCCGGTGTTCCCCAGTGGCCAACCTCTG‑GCGTTCTTCCACGGCAACGACAAGCAAGCCAAGGACTCAGGGCAGGGCGGCATGCAGGTGCGCTGgc                                                                                                                                                 >  7:14553/1‑149 (MQ=255)
   tttCTCCACAGACGACTTCTTTGGCGATGGCGCCGAGCGCATGTTCGTCGGCGCGCCGGTGTTCCCCAGTGGCCAACCTCTG‑GCGTTCTTCCACGGCAACGACAAGCAAGCCAAGGACTCAGGGCAGGGCGGCATGCAGGTGCGCTGgc                                                                                                                                                 >  2:329583/1‑149 (MQ=255)
        ccACAGACGACTTCTTTGGCGATGGCGCCGAGCGCATGTTCGTCGGCGCGCCGGTGTTCCCCAGTGGCCAACCTCTG‑GCGTTCTTCCACGGCAACGACAAGCAAGCCAAGGACTCAGGGCAGGGCGGCATGCAGGTGCGCTGGCGCAcc                                                                                                                                            >  2:126143/1‑149 (MQ=255)
         cacaGACGACTTCTTTGGCGATGGCGCCGAGCGCATGTTCGTCGGCGCGCCGGTGTTCCCCAGTGGCCAACCTCTG‑GCGTTCTTCCACGGCAACGACAAGCAAGCCAAGGACTCAGGGCAGGGCGGCATGCAGGTGCGCTGGCGCAcc                                                                                                                                            <  6:312810/148‑1 (MQ=255)
            agccgacTTCTTTGTCGAGGGCGCCGAGCGCATGTTCGGCGGCGCGCCGGTGTTCCCCAGTGGCCAACCTCTA‑GCGTGCTTCCACGGCAACGACAAGCAAGCCAAGGACTCAGGGCAGGGCGGCATGCAGCTGCGCTGGCGCACCgaga                                                                                                                                        <  6:236744/146‑1 (MQ=255)
              acgacTTCTTTGGCGATGGCGCCGAGCGCATGTTCGTCGGCGCGCCGGTGTTCCCCAGTGGCCAACCTCTG‑GCGTTCTTCCACGGCAACGACAAGCAAGCCAAGGACTCAGGGCAGGGCGGCATGCAGGTGCGCTGGCGCACCGAGACg                                                                                                                                      <  3:325696/149‑1 (MQ=255)
               cgacTTCTTTGGCGATGGCGCCGAGCGCATGTTCGTCGGCGCGCCGGTGTTCCCCAGTGGCCAACCTCTG‑GCGTTCTTCCACGGCAACGACAAGCAAGCCAAGGACTCAGGGCAGGGCGGCATGCAGGTGCGCTGGCGCACCGAGACgg                                                                                                                                     <  1:329583/149‑1 (MQ=255)
                gacTTCTTTGGCGATGGCGCCGAGCGCATGTTCGTCGGCGCGCCGGTGTTCCCCAGTGGCCAACCTCTG‑GCGTTCTTCCACGGCAACGACAAGCAAGCCAAGGACTCAGGGCAGGGCGGCATGCAGGTGCGCTGGCGCACCGAGACGGt                                                                                                                                    >  2:265416/1‑149 (MQ=255)
                 acTTCTTTGGCGATGGCGCCGAGCGCATGTTCGTCGGCGCGCCGGTGTTCCCCAGTGGCCAACCTCTG‑GCGTTCTTCCACGGCAACGACAAGCAAGCCAAGGACTCAGGGCAGGGCGGCATGCAGGTGCGCTGGCGCACCGAGACGGTc                                                                                                                                   <  4:329725/149‑1 (MQ=255)
                   ttcttTGGCGATGGCGCCGAGCGCATGTTCGTCGGCGCGCCGGTGTTCCCCAGTGGCCAACCTCTGAGCGTTCTTCCACGGCAACGACAAGCAAGCCAAGGACTCAGGGCAGGGCGGCATGCAGGTGCGCTGGCGCACCGAGACGGTc                                                                                                                                   >  1:271518/1‑148 (MQ=255)
                   ttcttTGGCGATGGCGCCGAGCGCATGTTCGTCGGCGCGCCGGTGTTCCCCAGTGGCCAACCTCTG‑GCGTTCTTCCACGGCAACGACAAGCAAGCCAAGGACTCAGGGCAGGGCGGCATGCAGGTGCGCTGGCGCACCGAGACGGTCGa                                                                                                                                 >  1:176640/1‑149 (MQ=255)
                   ttcttTGGCGATGGCGCCGAGCGCATGTTCGTCGGCGCGCCGGTGTTCCCCAGTGGCCAACCTCTG‑GCGTTCTTCCACGGCAACGACAAGCAAGCCAAGGACTCAGGGCAGGGCGGCATGCAGGTGCGCTGGCGCACCGAGACGGTCGa                                                                                                                                 >  5:127606/1‑149 (MQ=255)
                   ttcttTGGCGATGGCGCCGAGCGCATGTTCGTCGGCGCGCCGGTGTTCCCCAGTGGCCAACCTCTG‑GCGTTCTTCCACGGCAACGACAAGCAAGCCAAGGACTCAGGGCAGGGCGGCATGCAGGTGCGCCAGCGCAcctgcat                                                                                                                                       >  4:182435/1‑138 (MQ=255)
                   ttcttTGGCGATGGCGCCGAGCGCATGTTCGTCGGCGCGCCGGTGTTCCCCAGTGGCCAACCTCTG‑GCGTTCTTCCACGGCAACGACAAGCAAGCCAAGGACTCAGGGCAGGGCGGCATGCAGGTGCGCCAGCGCAcctgcat                                                                                                                                       <  3:182435/143‑6 (MQ=255)
                     cttTGGCGATGGCGCCGAGCGCATGTTCGTCGGCGCGCCGGTGTTCCCCAGTGGCCAACCTCTG‑GCGTTCTTCCACGGCAACGACAAGCAAGCCAAGGACTCAGGGCAGGGCGGCATGCAGGTGCGCCAGCGCAcctg                                                                                                                                          <  2:261173/138‑3 (MQ=255)
                     cttTGGCGATGGCGCCGAGCGCATGTTCGTCGGCGCGCCGGTGTTCCCCAGTGGCCAACCTCTG‑GCGTTCTTCCACGGCAACGACAAGCAAGCCAAGGACTCAGGGCAGGGCGGCATGCAGGTGCGCCAGCGCAcctg                                                                                                                                          >  1:261173/1‑136 (MQ=255)
                        tGGCGATGGCGCCGAGCGCATGTTCGTCGGCGCGCCGGTGTTCCCCAGTGGCCAACCTCTG‑GCGTTCTTCCACGGCAACGACAAGCAAGCCAAGGACTCAGGGCAGGGCGGCATGCAGGTGCGCTGGCGCACCGAGACGGTCGACTgg                                                                                                                             <  7:25941/148‑1 (MQ=255)
                         ggCGATGGCGCCGAGCGCATGTTCGTCGGCGCGCCGGTGTTCCCCAGTGGCCAACCTCTG‑GCGTTCTTCCACGGCAACGACAAGCAAGCCAAGGACTCAGGGCAGGGCGGCATGCAGGTGCGCTGGCGCACCGAGACGGTCGACTggg                                                                                                                            <  6:168985/148‑1 (MQ=255)
                         ggCGATGGCGCCGAGCGCATGTTCGTCGGCGCGCCGGTGTTCCCCAGTGGCCAACCTCTG‑GCGTTCTTCCACGGCAACGACAAGCAAGCCAAGGACTCAGGGCAGGGCGGCATGCAGGTGCGCTGGCGCACCGAGACGGTCGACTggg                                                                                                                            <  5:176542/148‑1 (MQ=255)
                          gCGATGGCGCCGAGCGCATGTTCGTCGGCGCGCCGGTGTTCCCCAGTGGCCAACCTCTG‑GCGTTCTTCCACGGCAACGACAAGCAAGCCAAGGACTCAGGGCAGGGCGGCATGCAGGTGCGCTGGCGCACCGAGACGGTCGACTGGGGc                                                                                                                          <  4:67435/149‑1 (MQ=255)
                          gCGATGGCGCCGAGCGCATGTGCGTCGGCGCGCCGGTGTTCCCCAGTGGCCAACCTCTG‑GCGTTCTTCCACGGCAACGACAAGCAAGCCAAGGACTCAGGGCAGGGCGGCATGCAGGTGCGCTGGCGCACCGAGACGGTCGACTGGGGc                                                                                                                          <  2:162704/149‑1 (MQ=255)
                                gcgcCGAGCGCATGTTCGTCGGCGCGCCGGTGTTCCCCAGTGGCCAACCTCTG‑GCGTTCTTCCACGGCAACGACAAGCAAGCCAAGGACTCAGGGCAGGGCGGCATGCAGGTGCGCTGGCGCACCGAGACGGTCGACTGGGGCCTGTa                                                                                                                     <  6:127606/148‑1 (MQ=255)
                                           aTGTTCGTCGGCGCGCCGGTGTTCCCCAGTGGCCAACCTCTG‑GCGTTCTTCCACGGCAACGACAAGCAAGCCAAGGACTCAGGGCAGGGCGGCATGCAGGTGCGCTGGCGCACCGAGACGGTCGACTGGGGCCTGTACGCCATCCGttt                                                                                                         >  5:30728/1‑149 (MQ=255)
                                               tcgtcgGCGCGCCGGTGTTCCCCAGTGGCCAACCTCTG‑GCGTTCTTCCACGGCAACGACAAGCAAGCCAAGGACTCAGGGCAGGGCGGCATGCAGGTGCGCTGGCGCACCGAGACGGTCGACTGGGGCCTGTACGCCATCCGTTTCCAc                                                                                                     >  5:181040/1‑149 (MQ=255)
                                               tcgtcgGCGCGCCGGTGTTCCCCAGTGGCCAACCTCTG‑GCGTTCTTCCACGGCAACGACAAGCAAGCCAAGGACTCAGGGCAGGGCGGCATGCAGGTGCGCTGGCGCACCGAGACGGTCGACTGGGGCCTGTACGCCATCCGTTTCCAc                                                                                                     >  2:90043/1‑149 (MQ=255)
                                                cgtcgGCGCGCCGGTGTTCCCCAGTGGCCAACCTCTG‑GCGTTCTTCCACGGCAACGACAAGCAAGCCAAGGACTCAGGGCAGGGCGGCATGCAGGTGCGCTGGCGCACCGAGACGGTCGACTGGGGCCTGTACGCCATCCGTTTCCAc                                                                                                     <  2:176640/148‑1 (MQ=255)
                                                       gcgcCGGTGTTCCCCAGTGGCCAACCTCTG‑GCGTTCTTCCACGGCAACGACAAGCAAGCCAAGGACTCAGGGCAGGGCGGCATGCAGGTGCGCTGGCGCACCGAGACGGTCGACTGGGGCCTGTACGCCATCCGTTTCCACGACAAGTc                                                                                             >  3:260015/1‑149 (MQ=255)
                                                          ccGGTGTTCCCCAGTGGCCAACCTCTG‑GCGTTCTTCCACGGCAACGACAAGCAAGCCAAGGACTCAGGGCAGGGCGGCATGCAGGTGCGCTGGCGCACCGAGACGGTCGACTGGGGCCTGTACGCCATCCGTTTCCACGACAAGTcgcc                                                                                          <  2:121418/149‑1 (MQ=255)
                                                             gtgtTCCCCAGTGGCCAACCTCTG‑GCATTCTTCCACGGCAACGACAAGCAAGCCAAGGACTCAGGGCAGGGCGGCATGCAGGTGCGCTGGCGCACCGAGACGGTCGACTGGGGCCTGTACGCCATCCGTTTCCAc                                                                                                     <  2:293386/135‑1 (MQ=255)
                                                             gtgtTCCCCAGTGGCCAACCTCTG‑GCATTCTTCCACGGCAACGACAAGCAAGCCAAGGACTCAGGGCAGGGCGGCATGCAGGTGCGCTGGCGCACCGAGACGGTCGACTGGGGCCTGTACGCCATCCGTTTCCAc                                                                                                     >  1:293386/1‑135 (MQ=255)
                                                              tgtTCCCCAGTGGCCAACCTCTG‑GCGTTCTTCCACGGCAACGACAAGCAAGCCAAGGACTCAGGGCAGGGCGGCATGCAGGTGCGCTGGCGCACCGAGACGGTCGACTGGGGCCTGTACGCCATCCGTTTCCACGACAAGTCGCCGCaa                                                                                      <  3:160350/149‑1 (MQ=255)
                                                                ttCCCCAGTGGCCAACCTCTG‑GCGTTCTTCCACGGCAACGACAAGCACGCCAAGGACTCAGGGCAGGGCGGCATGCAGGTGCGCTGGCGCACCGAGACGGTCGACTGGGGCCTGTACGCCATCCGTTTCCACGACAAGTCGCCGCAACt                                                                                    <  2:236350/149‑1 (MQ=255)
                                                                  ccccAGTGGCCAACCTCTG‑GCGTTCTTCCACGGCAACGACAAGCAAGCCAAGGACTCAGGGCAGGGCGGCATGCAGGTGCGCTGGCGCACCGAGACGGTCGACTGGGGCCTGTACGCCATCCGTTTCCACGACAAGTCGCCGCAACTc                                                                                   >  3:204009/1‑148 (MQ=255)
                                                                             aaCCTCTG‑GCGTTCTTCCACGGCAACGACAAGCAAGCCAAGGACTCAGGGCAGGGCGGCATGCAGGTGCGCTGGCGCACCGAGACGGTCGACTGGGGCCTGTACGCCATCCGTTTCCACGACAAGTCGCCGCAACTCAATGTACGccc                                                                        >  2:263602/1‑148 (MQ=255)
                                                                                  ctG‑GCGTTCTTCCACGGCAACGACAAGCAAGCCAAGGACTCAGGGCAGGGCGGCATGCAGGTGCGCTGGCGCACCGAGACGGTCGACTGGGGCCTGTa                                                                                                                     >  8:268085/1‑98 (MQ=255)
                                                                                  ctG‑GCGTTCTTCCACGGCAACGACAAGCAAGCCAAGGACTCAGGGCAGGGCGGCATGCAGGTGCGCTGGCGCACCGAGACGGTCGACTGGGGCCTGTa                                                                                                                     <  7:268085/98‑1 (MQ=255)
                                                                                  ctG‑GCGTTCTTCCACGGCAACGACAAGCAAGCCAAGGACTCAGGGCAGGGCGGCATGCAGGTGCGCTGGCGCACCGAGACGGTCGACTGGGGCCTGTACGCCATCCGTTTCCACGACAAGTCGCCGCAACTCAATGTACGCCCGAACtt                                                                  >  4:82183/1‑149 (MQ=255)
                                                                                      gCGTTCTTCCACGGCAACGACAAGCAAGCCAAGGACTCAGGGCAGGGCGGCATGCAGGTGCGCTGGCGCACCGAGACGGTCGACTGGGGCCTGTACGCCATCCGTTTCCACGACAAGTCGCCGCAACTCAATGTACGCCCGAACTTCGc                                                               <  6:30728/149‑1 (MQ=255)
                                                                                              ccACGGCAACGACAAGCAAGCCAAGGACTCAGGGCAGGGCGGCATGCAGGTGCGCTGGCGCACCGAGACGGTCGACTGGGGCCTGTACGCCATCCGTTTCCACGACAAGTCGCCGCAACTCAATGTACGCCCGAACTTCGCCAAcc                                                          <  8:14553/146‑1 (MQ=255)
                                                                                                aCGGCAACGACAAGCAAGCCAAGGACTCAGGGCAGGGCGGCATGCAGGTGCGCTGGCGCACCGAGACGGTCGACTGGGGCCTGTACGCCATCCGTTTCCACGACAAGTCGCCGCAACTCAATGTACGCCCGAACTTCGCCAACCTCGAc                                                     <  1:90043/149‑1 (MQ=255)
                                                                                                 cGGCAACGACAAGCAAGCCAAGGACTCAGGGCAGGGCGGCATGCAGGTGCGCTGGCGCACCGAGACGGTCGACTGGGGCCTGTACGCCATCCGTTTCCACGACAAGTCGCCGCAACTCAATGTACGCCCGAACTTCGCCAACCTCGAcc                                                    <  6:181040/149‑1 (MQ=255)
                                                                                                       cGACAAGCAAGCCAAGGACTCAGGGCAGGGCGGCATGCAGGTGCGCTGGCGCACCGAGACGGTCGACTGGGGCCTGTACGCCATCCGTTTCCACGACAAGTCGCCGCAACTCAATGTACGCCCGAACTTCGCCAACCTCGACCCGGTCa                                              <  3:252026/149‑1 (MQ=255)
                                                                                                            agcaagcCAAGGACTCAGGGCAGGGCGGCATGCAGGTGCGCTGGCGCACCGAGACGGTCGACTGGGGCCTGTACGCCATCCGTTTCCACGACAAGTCGCCGCAACTCAATGTACGCCCGAACTTCGCCAACCTCGACCCGGTCAGCgg                                          >  6:218208/1‑148 (MQ=255)
                                                                                                            agcaagcCAAGGACTCAGGGCAGGGCGGCATGCAGGTGCGCTGGCGCACCGAGACGGTCGACTGGGGCCTGTACGCCATCCGTTTCCACGACAAGTCGCCGCAACTCAATGTACGCCCGAACTTCGCCAACCTCGACCCGGTCAGCGGc                                         <  4:260015/149‑1 (MQ=255)
                                                                                                            agcaagcCAAGGACTCAGGGCAGGGCGGCATGCAGGTGCGCTGGCGCACCGAGACGGTCGACTGGGGCCTGTACGCCATCCGTTTCCACGACAAGTCGCCGCAACTCAATGTACGCCCGAACTTCGCCAACCTCGACCCGGTCAGCGGc                                         <  1:126143/149‑1 (MQ=255)
                                                                                                               aagcCAAGGACTCAGGGCAGGGCGGCATGCAGGTGCGCTGGCGCACCGAGACGGTCGACTGGGGCCTGTACGCCATCCGTTTCCACGACAAGTCGCCGCAACTCAATGTACGCCCGAACTTCGCCAACCTCGACCCGGTCAGCGGCAAg                                      <  1:265416/149‑1 (MQ=255)
                                                                                                                  ccAAGGACTCAGGGCAGGGCGGCATGCAGGTGCGCTGGCGCACCGAGACGGTCGACTGGGGCCTGTACGCCATCCGTTTCCACGACAAGTCGCCGCAACTCAATGTACGCCCGAACTTCGCCAACCTCGACCCGGTCAGCGGCAAGGcc                                   >  2:139779/1‑149 (MQ=255)
                                                                                                                  ccAAGGACTCAGGGCAGGGCGGCATGCAGGTGCGCTGGCGCACCGAGACGGTCGACTGGGGCCTGTACGCCATCCGTTTCCACGACAAGTCGCCGCAACTCAATGTACGCCCGAACTTCGCCAACCTCGACCCGGTCAGCGGCAAGGcc                                   >  2:55045/1‑149 (MQ=255)
                                                                                                                    aaGGACTCAGGGCAGGGCGGCATGCAGGTGCGCTGGCGCACCGAGACGGTCGACTGGGGCCTGTACGCCATCCGTTTCCACGACAAGTCGCCGCAACTCAATGTACGCCCGAACTTCGCCAACCTCGACCCGGTCAGCGGCAAGGCCgg                                 >  3:221816/1‑149 (MQ=255)
                                                                                                                     aGGACTCAGGGCAGGGCGGCATGCAGGTGCGCTGGCGCACCGAGACGGTCGACTGGGGCCTGTACGCCATCCGTTTCCACGACAAGTCGCCGCAACTCAATGTACGCCCGAACTTCGCCAACCTCGACCCGGTCAGCGGCAAGGCCGGc                                >  2:156622/1‑149 (MQ=255)
                                                                                                                       gACGCAGGGCAGGGCGGCATGCAGGTGCGCTGGCGCACCGAGACGGGCGACTGGGGCCTGTACGCCATCCGTTTCCACGCCAAGTCGCCGCAACTCAATGTACGCCCGAACTTCGCCAACCTCGACCCGGTCAGCGGCAAGGCCGGCGa                              <  2:99807/149‑1 (MQ=255)
                                                                                                                          tCAGGGCAGGGCGGCATGCAGGTGCGCTGGCGCACCGAGACGGTCGACTGGGGCCTGTACGCCATCCGTTTCCACGACAAGTCGCCGCAACTCAATGTACGCCCGAACTTCGCCAACCTCGACCCGGTCAGCGGCAAGGCCGGCGAGtt                           >  3:222555/1‑149 (MQ=255)
                                                                                                                             gggcagggcGGCATGCAGGTGCGCTGGCGCACCGAGACGGTCGACTGGGGCCTGTACGCCATCCGTTTCCACGACAAGTCGCCGCAACTCAATGTACGCCCGAACTTCGCCAACCTCGACCCGGTCAGCGGCAAGGCCGGCGAGTTCTa                        <  3:82183/149‑1 (MQ=255)
                                                                                                                               gcagggcGGCATGCAGGTGCGCTGGCGCACCGAGACGGTCGACTGGGGCCTGTACGCCATCCGTTTCCACGACAAGTCGCCGCAACTCAATGTACGCCCGAACTTCGCCAACCTCGACCCGGTCAGCGGCAAGGCCGGCGAGTTCTACt                      >  4:106853/1‑149 (MQ=255)
                                                                                                                                 agggcGGCATGCAGGTGCGCTGGCGCACCGAGACGGTCGACTGGGGCCTGTACGCCATCCGTTTCCACGACAAGTCGCCGCAACTCAATGTACGCCCGAACTTCGCCAACCTCGACCCGGTCAGCGGCAAGGCCGGCGAGTTCTACTgg                    <  1:156622/149‑1 (MQ=255)
                                                                                                                                  gggcGGCATGCAGGTGCGCTGGCGCACCGAGACGGTCGACTGGGGCCTGTACGCCATCCGTTTCCACGACAAGTCGCCGCAACTCAATGTACGCCCGAACTTCGCCAACCTCTACCCGGTCAGCGGCAAGGCCGGCGAGTTCTACTggg                   <  1:55045/149‑1 (MQ=255)
                                                                                                                                        cATGCAGGTGCGCTGGCGCACCGAGACGGTCGACTGGGGCCTGTACGCCATCCGTTTCCACGACAAGTCGCCGCAACTCAATGTACGCCCGAACTTCGCCAACCTCGACCCGGTCAGCGGCAAGGCCGGCGAGTTCTACTGGGTGTATc             >  6:249346/1‑149 (MQ=255)
                                                                                                                                                   gcTGGCGCACCGAGACGGTCGACTGGGGCCTGTACGCCATCCGTTTCCACGACAAGTCGCCGCAACTCAATGTACGCCCGAACTTCGCCAACCTCGACCCGGTCAGCGGCAAGGCCGGCGAGTTCTACTGGGTGTATCCCGAGGGTATc  >  1:319770/1‑149 (MQ=255)
                                                                                                                                                    cTGGCGCACCGAGACGGTCGACTGGGGCCTGTACGCCATCCGTTTCCACGACAAGTCGCCGCAACTCAATGTACGCCCGAACTTCGCCAACCTCGACCCGGTCAGCGGCAAGGCCGGCGAGTTCTACTGGGTGTATCCCGAGGGTATc  >  6:239842/1‑148 (MQ=255)
                                                                                                                                                     |                                                                                                                                                  
CTATTTCTCCACAGACGACTTCTTTGGCGATGGCGCCGAGCGCATGTTCGTCGGCGCGCCGGTGTTCCCCAGTGGCCAACCTCTG‑GCGTTCTTCCACGGCAACGACAAGCAAGCCAAGGACTCAGGGCAGGGCGGCATGCAGGTGCGCTGGCGCACCGAGACGGTCGACTGGGGCCTGTACGCCATCCGTTTCCACGACAAGTCGCCGCAACTCAATGTACGCCCGAACTTCGCCAACCTCGACCCGGTCAGCGGCAAGGCCGGCGAGTTCTACTGGGTGTATCCCGAGGGTATC  >  NC_002947/3630174‑3630468

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑