Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,692,619 T→A 9.4% Q336L (CAG→CTG)  PP_3259 ← acyl‑CoA dehydrogenase‑related protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,692,6190TA 9.4% 181.0 / 7.7 64Q336L (CAG→CTG) PP_3259acyl‑CoA dehydrogenase‑related protein
Reads supporting (aligned to +/- strand):  ref base T (24/34);  new base A (2/4);  total (26/38)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.79e-01

GCCCCGGCGGGCACAGTTGGCGTGAGTCGAGCCAGGTCGCACGCGCCAGGTGCGCGCTTTGCTGCCAGGCTGCCCAAGGCGTGCCGCCCGCGGACTGGATCAGGTTCTGGCTCATAGTTGCCACACCCCGGCTGGCAACTGGGCCAGCTGCTGGCCCAGCATCGCCAGATCACGCTCGGCGTGGCTCTGACGCAGGAACACCGGCAGGTCGGCGCTCAGCCGGGCAAAATGACTGCTGCGACAGAACGGCGTGGCCCCCAAGGCACGTCCGACGTGGCTTATGACCTGCT  >  NC_002947/3692471‑3692760
                                                                                                                                                    |                                                                                                                                             
gcCCCGGCGGGCACAGTTGGCGTGAGTCGAGCCAGGTCGCACGCGCCAGGTGCGCGCTTTGCTGCCAGGCTGCCCAAGGCGTGCCGCCCGCGGACTGGATCAGGTTCTGGCTCATAGTTGCCACACCCCGGCTGGCAACTGGGCCAgct                                                                                                                                               >  3:224606/1‑149 (MQ=255)
 ccccGGCGGGCACAGTTGGCGTGAGTCGAGCCAGGTCGCACGCGCCAGGTGCGCGCTTTGCTGCCAGGCTGCCCAAGGCGTGCCGCCCGCGGACTGGATCAGGTTCTGGCTCATAGTTGCCACACCCCGGCTGGCAACTGGGCCAgctg                                                                                                                                              >  6:213973/1‑149 (MQ=255)
 ccccGGCGGGCACAGTTGGCGTGAGTCGAGCCAGGTCGCACGCGCCAGGTGCGCGCTTTGCTGCCAGGCTGCCCAAGGCGTGCCGCCCGCGGACTGGATCAGGTTCTGGCTCATAGTTGCCACACCCCGGCTGGCAACTGGGCCAgctg                                                                                                                                              >  1:62450/1‑149 (MQ=255)
  cccGGCGGGCACAGTTGGCGTGAGTCGAGCCAGGTCGCACGCGCCAGGTGCGCGCTTTGCTGCCAGGCTGCCCAAGGCGTGCCGCCCGCGGACTGGATCAGGTTCTGGCTCATAGTTGCCACACCCCGGCTGGCAACTGGGCCAgctgc                                                                                                                                             <  4:301774/149‑1 (MQ=255)
   ccGGCGGGCACAGTTGGCGTGAGTCGAGCCAGGTCGCACGCGCCAGGTGCGCGCTTTGCTGCCAGGCTGCCCAAGGCGTGCCGCCCGCGGACTGGATCAGGTTCTGGCTCATAGTTGCCACACCCCGGCTGGCAACTGGGCCAgctgct                                                                                                                                            >  5:120235/1‑149 (MQ=255)
    cggcggGCACAGTTGGCGTGAGTCGAGCCAGGTCGCACGCGCCAGGTGCGCGCTTTGCTGCCAGGCTGCCCAAGGCGTGCCGCCCGCGGACTGGATCAGGTTCTGGCTCATAGTTGCCACACCCCGGCTGGCAACTGGGCCAGctgctg                                                                                                                                           <  4:150974/149‑1 (MQ=255)
      gcggGCACAGTTGGCGTGAGTCGAGCCAGGTCGCACGCGCCAGGTGCGCGCTTTGCTGCCAGGCTGCCCAAGGCGTGCCGCCCGCGGACTGGATCAGGTTCTGGCTCATAGTTGCCACACCCCGGCTGGCAGCTGGCCCAgttgccagc                                                                                                                                         <  4:59177/149‑5 (MQ=255)
       cggGCACAGTTGGCGTGAGTCGAGCCAGGTCGCACGCGCCAGGTGCGCGCTTTGCTGCCAGGCTGCCCAAGGCGTGCCGCCCGCGGACTGGATCAGGTTCTGGCTCATAGTTGCCACACCCCGGCTGGCAACTGGGCCAGCAGCTGGcc                                                                                                                                        <  1:131472/149‑1 (MQ=255)
        gggCACAGTTGGCGTGAGTCGAGCCAGGTCGCACGCGCCAGGTGCGCGCTTTGCTGCCAGGCTGCCCAAGGCGTGCCGCCCGCGGACTGGATCAGGTTCTGGCTCATAGTTGCCACACCCCGGCTGGCAACTGGGCCAGCTGCTGGccc                                                                                                                                       <  2:94558/149‑1 (MQ=255)
         ggCACAGTTGGCGTGAGTCGAGCCAGGTCGCACGCGCCAGGTGCGCGCTTTGCTGCCAGGCTGCCCAAGGCGTGCCGCCCGCGGACTGGATCAGGTTCTGGCTCATAGTTGCCACACCCCGGCTGGCAACTGGGCCAGCTGCTGGCCCa                                                                                                                                      >  8:31963/1‑149 (MQ=255)
         ggCACAGTTGGCGTGAGTCGAGCCAGGTCGCACGCGCCAGGTGCGCGCTTTGCTGCCAGGCTGCCCAAGGCGTGCCGCCCGCGGACTGGATCAGGTTCTGGCTCATAGTTGCCACACCCCGGCTGGCAACTGGGCCAGCTGCTGGCCCa                                                                                                                                      <  6:120235/149‑1 (MQ=255)
          gCACAGTTGGCGTGAGTCGAGCCAGGTCGCACGCGCCAGGTGCGCGCTTTGCTGCCAGGCTGCCCAAGGCGTGCCGCCCGCGGACTGGATCAGGTTCTGGCTCATAGTTGCCACACCCCGGCTGGCAACTGGGCCAGCAGCTGGCCCAg                                                                                                                                     <  5:50917/149‑1 (MQ=255)
              aGTTGGCGTGAGTCGAGCCAGGTCGCACGCGCCAGGTGCGCGCTTTGCTGCCAGGCTGCCCAAGGCGTGCCGCCCGCGGACTGGATCAGGTTCTGGCTCATAGTTGCCACACCCCGGCTGGCAACTGGGCCAGCTGCTGGCCCAGCATc                                                                                                                                 >  7:229793/1‑149 (MQ=255)
                ttGGCGTGAGTCGAGCCAGGTCGCACGCGCCAGGTGCGCGCTTTGCTGCCAGGCTGCCCAAGGCGTGCCGCCCGCGGACTGGATCAGGTTCTGGCTCATAGTTGCCACACCCCGGCTGGCAACTGGGCCAGCTGCTGGCCCAGCATCGc                                                                                                                               <  5:122230/149‑1 (MQ=255)
                      tGAGTCGAGCCAGGTCGCACGCGCCAGGTGCGCGCTTTGCTGCCAGGCTGCCCAAGGCGTGCCGCCCGCGGACTGGATCAGGTTCTGGCTCATAGTTGCCACACCCCGGCTGGCAACTGGGCCAGCTGCTGGCCCAGCATCGCCAGAt                                                                                                                          >  3:162176/1‑148 (MQ=255)
                            gAGCCAGGTCGCACGCGCCAGGTGCGCGCTTTGCTGCCAGGCTGCCCAAGGCGTGCCGCCCGCGGACTGGATCAGGTTCTGGCTCATAGTTGCCACACCCCGGCTGGCAACTGGGCCAGCTGCTGGCCCAGCATCGCCAGATCACGCTc                                                                                                                   >  2:244617/1‑149 (MQ=255)
                            gAGCCAGGTCGCACGCGCCAGGTGCGCGCTTTGCTGCCAGGCTGCCCAAGGCGTGCCGCCCGCGGACTGGATCAGGTTCTGGCTCATAGTTGCCACACCCCGGCTGGCAACTGGGCCAGCTGCTGGCCCAGCATCGCCAGATCACGCTc                                                                                                                   <  7:43029/149‑1 (MQ=255)
                               ccAGGTCGCACGCGCCAGGTGCGCGCTTTGCTGCCAGGCTGCCCAAGGCGTGCCGCCCGCGTACTGGATCAGGTTCTGGCTCATAGTTGCCACACCCCGGCTGGCAACTGGGCCAGCTGCTGGCCCAGCATCGCCAGATCACGCTCGGc                                                                                                                <  8:292335/149‑1 (MQ=255)
                                 aGGTCGCACGCGCCAGGTGCGCGCTTTGCTGCCAGGCTGCCCAAGGCGTGCCGCCCGCGGACTGGATCAGGTTCTGGCTCATAGTTGCCACACCCCGGCTGGCAACTGGGCCAGCTGCTGGCCCAGCATCGCCAGATCACTCTCGGCGt                                                                                                              <  4:224606/149‑1 (MQ=255)
                                   gTCGCACGCGCCAGGTGCGCGCTTTGCTGCCAGGCTGCCCAAGGCGTGCCGCCCGCGGACTGGATCAGGTTCTGGCTCATAGTTGCCACACCCCGGCTGGCAACTGGGCCAGCTGCTGGCCCAGCATCGCCAGATCACGCTCGGCGTgg                                                                                                            <  8:229793/149‑1 (MQ=255)
                                    tCGCACGCGCCAGGTGCGCGCTTTGCTGCCAGGCTGCCCAAGGCGTGCCGCCCGCGGACTGGATCAGGTTCTGGCTCATAGTTGCCACACCCCGGCTGGCAACTGGGCCAGCTGCTGGCCCAGCATCGCCAGATCACGCTCGGCGTGGc                                                                                                           >  2:69451/1‑149 (MQ=255)
                                        aCGCGCCAGGTGCGCGCTTTGCTGCCAGGCTGCCCAATGCGTGCCGCCCGCGGTCTTGATCAGTTTCTGGCTCATAGTTGCCACACCCCGGCTGGCAACTGGGCCAGCTGCTGGCCCAGCATCGCCAGATCACGCTCGGCGTGGCTCTg                                                                                                       <  1:280625/149‑1 (MQ=255)
                                        aCGCGCCAGGTGCGCGCTTTGCTGCCAGGCTGCCCAAGGCGTGCCGCCCGCGGACTGGATCAGGTTCTGGCTCATAGTTGCCACACCCCGGCTGGCAACTGGGCCAGCTGCTGGCCCAGCATCGCCAGATCACGCTCGGCGTGGctct                                                                                                        >  6:320113/1‑148 (MQ=255)
                                        aCGCGCCAGGTGCGCGCTTTGCTGCCAGGCTGCCCAAGGCGTGCCGCCCGCGGACTGGATCAGGTTCTGGCTCATAGTTGCCACACCCCGGCTGGCAACTGGGCCAGCTGCTGGCCCAGCATCGCCAGATCACGCTCGGCGTGGCTCTg                                                                                                       <  1:69451/149‑1 (MQ=255)
                                         cgcgCCAGGTGCGCGCTTTGCTGCCAGGCTGCCCAAGGCGTGCCGCCCGCGGACTGGATCAGGTTCTGGCTCATAGTTGCCACACCCCGGCTGGCAACTGGGCCAGCTGCTGGCCCAGCATCGCCAGATCACGCTCGGCGTGGCTCTGa                                                                                                      <  5:213973/149‑1 (MQ=255)
                                                ggTGCGCGCTTTGCTGCCAGGCTGCCCAAGGCGTGCCGCCCGCGGACTGGATCAGGTTCTGGCTCATAGTTGCCACACCCCGGCTGGCAACTGGGCCAGCTGCTGGCCCAGCATCGCCAGATCACGCTCGGCGTGGCTCTGACGCAGGa                                                                                               <  4:93036/149‑1 (MQ=255)
                                                 gTGCGCGCTTTGCTGCCAGGCTGCCCAAGGCGTGCCGCCCGCGGACTGGATCAGGTTCTGGCTCATAGTTGCCACACCCCGGCTGGCAACTGGGCCAGCTGCTGGCCCAGCATCGCCAGATCACGCTCGGCGTGGCTCTGACGCAGGaa                                                                                              >  8:153715/1‑149 (MQ=255)
                                                 gTGCGCGCTTTGCTGCCAGGCTGCCCAAGGCGTGCCGCCCGCGGACTGGATCAGGTTCTGGCTCATAGTTGCCACACCCCGGCTGGCAACTGGGCCAGCTGCTGGCCCAGCATCGCCAGATCACGCTCGGCGTGGCTCTGACGCAGGaa                                                                                              <  1:229461/149‑1 (MQ=255)
                                                 gTGCGCGCTTTGCTGCCAGGCTGCCCAAGGCGTGCCGCCCGCGGACTGGATCAGGTTCTGGCTCATAGTTGCCACACCCCGGCTGGCAACTGGGCCAGCTGCTGGCCCAGCATCGCCAGATCACGCTCGGCGTGGCTCTGACGCAGGaa                                                                                              >  3:194997/1‑149 (MQ=255)
                                                     gcgcTTTGCTGCCAGGCTGCCCAAGGCGTGCCGCCCGCGGACTGGATCAGGTTCTGGCTCATAGTTGCCACACCCCGGCTGGCAACTGGGCCAGCTGCTGGCCCAGCATCGCCAGATCACGCTCGGCGTGGCTCTGAc                                                                                                     <  6:332444/138‑1 (MQ=255)
                                                     gcgcTTTGCTGCCAGGCTGCCCAAGGCGTGCCGCCCGCGGACTGGATCAGGTTCTGGCTCATAGTTGCCACACCCCGGCTGGCAACTGGGCCAGCTGCTGGCCCAGCATCGCCAGATCACGCTCGGCGTGGCTCTGAc                                                                                                     >  5:332444/1‑138 (MQ=255)
                                                        cTTTGCTGCCAGGCTGCCCAAGGCGTGCCGCCCGCGGACTGGATCAGGTTCTGGCTCATAGTTGCCACACCCCGGCTGGCAACTGGGCCAGCTGCTGGCCCAGCATCGCCAGATCACGCTCGGCGTGGCTCTGACGCAGGAACACCGGc                                                                                       <  4:194997/149‑1 (MQ=255)
                                                             ctgcCAGGCTGCCCAAGGCGTGCCGCCCGCGGACTGGATCAGGTTCTGGCTCATAGTTGCCACACCCCGGCTGGCAACTGGGCCAGCTGCTGGCCCAGCATCGCCAGATCACGCTCGGCGTGGCTCTGACGCAGGAACACCGGCAGGt                                                                                   <  7:31963/148‑1 (MQ=255)
                                                             ctgcCAGGCTGCCCAAGGCGTGCCGCCCGCGGACTGGATCAGGTTCTGGCTCATAGTTGCCACACCCCGGCTGGCAACTGGGCCAGCTGCTGGCCCAGCATCGCCAGATCACGCTCGGCGTGGCTCTGACGCAGGAACACCGGCAGGt                                                                                   <  2:62450/148‑1 (MQ=255)
                                                                ccAGGCTGCCCAAGGCGTGCCGCCCGCGGACTGGATCAGGTTCTGGCTCATAGTTGCCACACCCCGGCTGGCAACTGGGCCAGCTGCTGGCCCAGCATCGCCAGATCACGCTCGGCGTGGCTCTGACGCAGGAACACCGGCAGGTCGgc                                                                               >  2:241998/1‑149 (MQ=255)
                                                                ccAGGCTGCCCAAGGCGTGCCGCCCGCGGACTGGATCAGGTTCTGGCTCATAGTTGCCACACCCCGGCTGGCAACTGGGCCAGCTGCTGGCCCAGCATCGCCAGATCACGCTCGGCGTGGCTCTGACGCAGGAACACCGGCAGGTCGgc                                                                               <  4:162176/149‑1 (MQ=255)
                                                                   ggCTGCCCAAGGCGTGCCGCCCGCGGACTGGATCAGGTTCTGGCTCATAGTTGCCACACCCCGGCTGGCAACTGGGCCAGCTGCTGGCCCAGCATCGCCAGATCACGCTCGGCGTGGCTCTGACGCAGGAACACCGGCAGGTCGGCGCt                                                                            >  8:151531/1‑149 (MQ=255)
                                                                         ccAAGGCGTGCCGCCCGCGGACTGGATCAGGTTCTGGCTCATAGTTGCCACACCCCGGCTGGCAACTGGGCCAGCTGCTGGCCCAGCATCGCCAGATCACGCTCGGCGTGGCTCTGACGCAGGAACACCGGCAGGTCGGCGCTCAGCCg                                                                      >  3:170419/1‑149 (MQ=255)
                                                                         ccAAGGCGTGCCGCCCGCGGACTGGATCAGGTTCTGGCTCATAGTTGCCACACCCCGGCTGGCAACTGGGCCAGCAGCTGGcc                                                                                                                                        >  1:5849/1‑83 (MQ=255)
                                                                         ccAAGGCGTGCCGCCCGCGGACTGGATCAGGTTCTGGCTCATAGTTGCCACACCCCGGCTGGCAACTGGGCCAGCAGCTGGcc                                                                                                                                        <  2:5849/83‑1 (MQ=255)
                                                                          cAAGGCGTGCCGCCCGCGGACTGGATCAGGTTCTGGCTCATAGTTGCCACACCCCGGCTGGCAACTGGGCCAGCTGCTGGCCCAGCATCGCCAGATCACGCTCGGCGTGGCTCTGACGCAGGAACACCGGCAGGTCGGCGCTCAGCCgg                                                                     <  5:320113/149‑1 (MQ=255)
                                                                          cAAGGCGTGCCGCCCGCGGACTGGATCAGGTTCTGGCTCATAGTTGCCACACCCCGGCTGGCAACTGGGCCAGCTGCTGGCCCAGCATCGCCAGATCACGCTCGGCGTGGCTCTGACGCAGGAACACCGGCAGGTCGGCGCTCAGCCgg                                                                     >  7:76240/1‑149 (MQ=255)
                                                                          cAAGGCGTGCCGCCCGCGGACTGGATCAGGTTCTGGCTCATAGTTGCCACACCCCGGCTGGCAACTGGGCCAGCTGCTGGCCCAGCATCGCCAGATCACGCTCGGCGTGGCTCTGACGCAGGAACACCGGCAGGTCGGCGCTCAGCCgg                                                                     >  5:218363/1‑149 (MQ=255)
                                                                          cAAGGCGTGCCGCCCGCGGACTGGATCAGGTTCTGGCTCATAGTTGCCACACCCCGGCTGGCAACTGGGCCAGCTGCTGGCCCAGCATCGCCAGATCACGCTCGGCGTGGCTCTGACGCAGGAACACCGGCAGGTCGGCGCTCAGCCgg                                                                     >  5:154613/1‑149 (MQ=255)
                                                                           aaGGCGTGCCGCCCGCGGACTGGATCAGGTTCTGGCTCATAGTTGCCACACCCCGGCTGGCAACTGGGCCAGCTGCTGGCCCAGCATCGCCAGATCACGCTCGGCGTGGCTCTGACGCAGGAACACCGGCAGGTCGGCGCTCAGCCggg                                                                    <  7:260382/149‑1 (MQ=255)
                                                                                   ccgctcgcTGTCTGGATGAGGTTCTGGCTCATAGTTTCCACACCGCGGCTGGCAACTGGGCCAGCTGCTGGCCCAGCATCGCCAGATCACGCTCGGCGTGGCTCTGACGCAGGAACAGCGGCAGGTCGGCGCTCAGCCGGGCAAAATGa                                                            <  7:77234/149‑1 (MQ=255)
                                                                                    cgcccgcGGACTGGATCAGGTTCTGGCTCATAGTTGCCACACCCCGGCTGGCAACTGGGCCAGCTGCTGGCCCAGCATCGCCAGATCACGCTCGGCGTGGCTCTGACGCAGGAACACCGGCAGGTCGGCGCTCAGCCGGGCAAAATGAc                                                           <  1:244617/149‑1 (MQ=255)
                                                                                     gcccgcGGACTGGATCAGGTTCTGGCTCATAGTTGCCACACCCCGGCTGGCAACTGGGCCAGCTGCTGGCCCAGCATCGCCAGATCACGCTCGGCGTGGCTCTGACGCAGGAACACCGGCAGGTCGGCGCTCAGCCGGGCAAAATGAct                                                          <  7:153715/149‑1 (MQ=255)
                                                                                                 gATCAGGTTCTGGCTCATAGTTGCCACACCCCGGCTGGCAACTGGGCCAGCTGCTGGCCCAGCATCGCCAGATCACGCTCGGCGTGGCTCTGACGCAGGAACACCGGCAGGTCGGCGCTCAGCCGGGCAAAATGACTGCTGCGACAGaa                                              >  4:110259/1‑149 (MQ=255)
                                                                                                  aTCAGGTTCTGGCTCATAGTTGCCACACCCCGGCTGGCAACTGGGCCAGCTGCTGGCCCAGCATCGCCAGATCACGCTCGGCGTGGCTCTGACGCAGGAACACAGGCAGGTCGGCGCTCAGCCGGGCAAAATGACTGCTGCGACAGAAc                                             <  1:241998/149‑1 (MQ=255)
                                                                                                       gTTCTGGCTCATAGTTGCCACACCCCGGCTGGCAACTGGGCCAGCTGCTGGCCCAGCATCGCCAGATCACGCTCGGCGTGGCTCTGACGCAGGAACACCGGCAGGTCGGCGCTCAGCCGGGCAAAATGActg                                                         >  8:149424/1‑132 (MQ=255)
                                                                                                       gTTCTGGCTCATAGTTGCCACACCCCGGCTGGCAACTGGGCCAGCTGCTGGCCCAGCATCGCCAGATCACGCTCGGCGTGGCTCTGACGCAGGAACACCGGCAGGTCGGCGCTCAGCCGGGCAAAATGActg                                                         <  7:149424/132‑1 (MQ=255)
                                                                                                       gTTCTGGCTCATAGTTGCCACACCCCGGCTGGCAACTGGGCCAGCTGCTGGCCCAGCATCGCCAGATCACGCTCGGCGTGGCTCTGACGCAGGAACACCGGCAGGTCGGCGCTCAGCCGGGCAAAATGACTGCTGCGACAGAACGGCGt                                        <  3:110259/149‑1 (MQ=255)
                                                                                                       gTTCTGGCTCATAGTTGCCACACCCCGGCTGGCAACTGGGCCAGCTGCTGGCCCAGCATCGCCAGATCACGCTCGGCGTGGCTCTGACGCAGGAACACCGGCAGGTCGGCGCTCAGCCGGGCAAAATGACTGCTGCGACAGAACGGCGt                                        >  3:317479/1‑149 (MQ=255)
                                                                                                             gCTCATAGTTGCCACACCCCGGCTGGCAACTGGGCCAGCTGCTGGCCCAGCATCGCCAGATCACGCTCGGCGTGGCTCTGACGCAGGAACACCGGCAGGTCGGCGCTCAGCCGGGCAAAATGACTGCTGCGACAGAACGGCGTGGccc                                   <  6:218363/148‑1 (MQ=255)
                                                                                                             gCTCATAGTTGCCACACCCCGGCTGGCAACTGGGCCAGCTGCTGGCCCAGCATCGCCAGATCACGCTCGGCGTGGCTCTGACGCAGGAACACCGGCAGGTCGGCGCTCAGCCGGGCAAAATGACTGCTGCGACAGAACGGCGTGGc                                     <  4:170419/146‑1 (MQ=255)
                                                                                                               tCATAGTTGCCACACCCCGGCTGGCAACTGGGCCAGCTGCTGGCCCAGCATCGCCAGATCACGCTCGGCGTGGCTCTGACGCAGGAACACCGGCAGGTCGGCGCTCAGCCGGGCAAAATGACTGCTGCGACAGAACGGCGTGGccccc                                 >  2:312064/1‑148 (MQ=255)
                                                                                                                 aTAGTTGCCACACCCCGGCTGGCAACTGGGCCAGCTGCTGGCCCAGCATCGCCAGATCACGCTCGGCGTGGCTCTGACGCAGGAACACCGGCAGGTCGGCGCTCAGCCGGGCAAAATGACTGCTGCGACAGAACGGCGTGGCCCCCaa                               <  1:312064/148‑1 (MQ=255)
                                                                                                                 aTAGTTGCCACACCCCGGCTGGCAACTGGGCCAGCTGCTGGCCCAGCATCGCCAGATCACGCTCGGCGTGGCTCTGACGCAGGAACACCGGCAGGTCGGCGCTCAGCCGGGCAAAATGACTGCTGCGACAGAACGGCGTGGCCCCCAAg                              >  1:63150/1‑149 (MQ=255)
                                                                                                                           cacCCCGGCTGGCAACTGGGCCAGCTGCTGGCCCAGCATCGCCAGATCACGCTCGGCGTGGCTCTGACGCAGGAACACCGGCAGGTCGGCGCTCAGCCGGGCAAAATGACTGCTGCGACAGAACGGCGTGGCCCCCAAGGCACGTCCGa                    <  7:151531/149‑1 (MQ=255)
                                                                                                                            acCCCGGCTGGCAACTGGGCCAGCTGCTGGCCCAGCATCGCCAGATCACGCTCGGCGTGGCTCTGACGCAGGAACACCGGCAGGTCGGCGCTCAGCCGGGCAAAATGACTGCTGCGACAGAACAGCGTTGCCCCCAAGGCACGTACGAc                   >  2:63365/1‑149 (MQ=255)
                                                                                                                                 ggCTGGCAACTGGGCCAGCTGCTGGCCCAGCATCGCCAGATCACGCTCGGCGTGGCTCTGACGCAGGAACACCGGCAGGTCGGCGCTCAGCCGGGCAAAATGACTGCTGCGACAGAACGGCGTGGCCCCCAAGGCACGTCCGACGTGGc              <  4:317479/149‑1 (MQ=255)
                                                                                                                                      gCAACTGGGCCAGCTGCTGGCCCAGCATCGCCAGATCACGCTCGGCGTGGCTCTGACGCAGGAACACCGGCAGGTCGGCGCTCAGCCGGGCAAAATGACTGCTGCGACAGAACGGCGTGGCCCCCAAGGCACGTCCGACGTGGCTTAt          >  3:19381/1‑148 (MQ=255)
                                                                                                                                             ggCCAGCTGCTGGCCCAGCATCGCCAGATCACGCTCGGCGTGGCTCTGACGCAGGAACACCGGCAGGTCGGCGCTCAGCCGGGCAAAATGACTGCTGCGACAGAACGGCGTGGCCCCCAAGGCACGTCCGACGTGGCTTATGACCTGct  <  6:154613/149‑1 (MQ=255)
                                                                                                                                             ggCCAGCTGCTGGCCCAGCATCGCCAGATCACGCTCGGCGTGGCTCTGACGCAGGAACACCGGCAGGTCGGCGCTCAGCCGGGCAAAATGACTGCTGCGACAGAACGGCGTGGCCCCCAAGGCACGTCCGACGTGGCTTATGACCTGc   <  8:76240/148‑1 (MQ=255)
                                                                                                                                             ggCCAGCAGCTGGCCCAGCATCGCCAGATCACGCTCGGCGTGGCTCTGACGCAGGAACACCGGCAGGTCGGCGCTCAGCCGGGCAAAATGACTGCTGCGACAGAACGGCGTGGCCCCCAAGGCACGTCCGACGTGGCTTATGAcc      >  7:176625/1‑145 (MQ=255)
                                                                                                                                             ggCCAGCAGCTGGCCCAGCATCGCCAGATCACGCTCGGCGTGGCTCTGACGCAGGAACACCGGCAGGTCGGCGCTCAGCCGGGCAAAATGACTGCTGCGACAGAACGGCGTGGCCCCCAAGGCACGTCCGACGTGGCTTATGAcc      <  8:176625/145‑1 (MQ=255)
                                                                                                                                                cAGCTGCTGGCCCAGCATCGCCAGATCACGCTCGGCGTGGCTCTGACGCAGGAACACCGGCAGGTCGGCGCTCAGCCGGGCAAAATGACTGCTGCGACAGAACGGCGTGGCCCCCAAGGCACGTCCGACGTGGc              >  3:313670/1‑134 (MQ=255)
                                                                                                                                                cAGCTGCTGGCCCAGCATCGCCAGATCACGCTCGGCGTGGCTCTGACGCAGGAACACCGGCAGGTCGGCGCTCAGCCGGGCAAAATGACTGCTGCGACAGAACGGCGTGGCCCCCAAGGCACGTCCGACGTGGc              <  4:313670/134‑1 (MQ=255)
                                                                                                                                                cAGCTGCTGGCCCAGCATCGCCAGATCACGCTCGGCGTGGCTCTGACGCAGGAACACCGGCAGGTCGGCGCTCAGCCGGGCAAAATGACTGCTGCGACAGAACGGCGTGGCCCCCAAGGCACGTCCGACGTGGCTTATGACCTGc   <  7:223790/145‑1 (MQ=255)
                                                                                                                                                cAGCTGCTGGCCCAGCATCGCCAGATCACGCTCGGCGTGGCTCTGACGCAGGAACACCGGCAGGTCGGCGCTCAGCCGGGCAAAATGACTGCTGCGACAGAACGGCGTGGCCCCCAAGGCACGTCCGACGTGGCTTATGACCTGc   >  8:223790/1‑145 (MQ=255)
                                                                                                                                                    |                                                                                                                                             
GCCCCGGCGGGCACAGTTGGCGTGAGTCGAGCCAGGTCGCACGCGCCAGGTGCGCGCTTTGCTGCCAGGCTGCCCAAGGCGTGCCGCCCGCGGACTGGATCAGGTTCTGGCTCATAGTTGCCACACCCCGGCTGGCAACTGGGCCAGCTGCTGGCCCAGCATCGCCAGATCACGCTCGGCGTGGCTCTGACGCAGGAACACCGGCAGGTCGGCGCTCAGCCGGGCAAAATGACTGCTGCGACAGAACGGCGTGGCCCCCAAGGCACGTCCGACGTGGCTTATGACCTGCT  >  NC_002947/3692471‑3692760

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑