Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,698,182 T→C 7.4% N97D (AAT→GAT)  ybhN ← phospholipid modification enzyme

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,698,1820TC 7.4% 152.4 / 2.8 54N97D (AAT→GAT) ybhNphospholipid modification enzyme
Reads supporting (aligned to +/- strand):  ref base T (27/23);  new base C (2/2);  total (29/25)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.74e-01

AAATACGGTGCCGGCCAGCAGCATATAGCCCATCCAGTTGGTCAACAGCCCCAAGGTGAGGATTCGTGTAATGGTCGCGGTATCCAGGCCCAGCCGCCCATAAAGGCGGTAGCGCAAGGCAACCCCGCCTACCCAGGTAGTGAAATTGAGGTTAAAGGCGTAGCACACGAACGCGACGGGCAATACCTGGCGGGCAGGTAGCGTATGGCCGGTGTAAGCCCGGGCCAACAGGTCGTAACTGGCAAACGTGAAGTAGCTGCACAACGCGATCGCCAAGCCGATGGCAAGGGTGC  >  NC_002947/3698036‑3698328
                                                                                                                                                  |                                                                                                                                                  
aaaTACGGTGCCGGCCAGCAGCATATAGCCCATCCAGTTGGTCAACAGCCCCAAGGTGAGGATTCGTGTAATGGTCGCGGTATCCAGGCCCAGCCGCCCATAAAGGCGGTAGCGCAAGGCAACCCCGCCTACCCAGGTAGTGAAAtt                                                                                                                                                    >  4:68326/1‑147 (MQ=255)
        tGCCGGCCAGCAGCATATAGCCCATCCAGTTGGTCAACAGCCCCAAGGTGAGGATTCGTGTAATGGTCGCGGTATCCAGGCCCAGCCGCCCATAAAGGCGGTAGCGCAAGGCAACCCCGCCTACCCAGGTAGTGAAATTGAGGTTAAAg                                                                                                                                          <  5:156208/149‑1 (MQ=255)
          ccGGCCAGCAGCATATAGCCCATCCAGTTGGTCAACAGCCCCAAGGTGAGGATTCGTGTAATGGTCGCGGTATCCAGGCCCAGCCGCCCATAAAGGCGGTAGCGCAAGGCAACCCCGCCTACCCAGGTAGTGAAATTGAGGTTAAAGGc                                                                                                                                        <  3:18174/149‑1 (MQ=255)
            ggCCAGCAGCATATAGCCCATCCAGTTGGTCAACAGCCCCAAGGTGAGGATTCGTGTAATGGTCGCGGTATCCAGGCCCAGCCGCCCATAAAGGCGGTAGCGCAAGGCAACCCCGCCTACCCAGGTAGTGAAATTGAGGTTAAAGGCg                                                                                                                                       >  6:230460/1‑148 (MQ=255)
              ccAGCAGCATATACCCCATCCAGTTGCTCAACAGCCCCAAGGTGAGGATTCGTGTAATGGTCGCGGTATCCAGGCCCAGCCGCCCATAAAGGCGGTAGCGCAAGGCAACCCCGCCTACCCAGGTAGTGAAATTGAGGTTAAAGGCGTAg                                                                                                                                    <  4:113308/149‑1 (MQ=255)
                agcagcATATAGCCCATCCAGTTGGTCAACAGCCCCAAGGTGAGGATTCGTGTAATGGTCGCGGTATCCAGGCCCAGCCGCCCATAAAGGCGGTAGCGCAAGGCAACCCCGCCTACCCAGGTAGTGAAATTGAGGTTAAAGGCGTAGca                                                                                                                                  >  8:267488/1‑149 (MQ=255)
                       tataGCCCATCCAGTTGGTCAACAGCCCCAAGGTGAGGATTCGTGTAATGGTCGCGGTATCCAGGCCCAGCCGCCCATAAAGGCGGTAGCGCAAGGCAGCCCCGCCTACCCAGGTAGTGAAATTGAGGTTAAAGGCGTAg                                                                                                                                    >  8:273037/1‑140 (MQ=255)
                       tataGCCCATCCAGTTGGTCAACAGCCCCAAGGTGAGGATTCGTGTAATGGTCGCGGTATCCAGGCCCAGCCGCCCATAAAGGCGGTAGCGCAAGGCAGCCCCGCCTACCCAGGTAGTGAAATTGAGGTTAAAGGCGTAg                                                                                                                                    <  7:273037/140‑1 (MQ=255)
                         taGCCCATCCAGTTGGTCAACAGCCCCAAGGTGAGGATTCGTGTAATGGTCGCGGTATCCAGGCCCAGCCGCCCATAAAGGCGGTAGCGCAAGGCAACCCCGCCTACCCAGGTAGTGAAATTGAGGTTAAAGGCGTAGCACACGAAcgc                                                                                                                         >  5:208795/1‑149 (MQ=255)
                                     ttGGTCAACAGCCCCAAGGTGAGGATTCGTGTAATGGTCGCGGTATCCAGGCCCAGCCGCCCATAAAGGCGGTAGCGCAAGGCAACCCCGCCTACCCAGGTAGTGAAATTGAGGTTAAAGGCGTAGCACACGAACGCGACGGGCAATAc                                                                                                             <  1:140327/149‑1 (MQ=255)
                                       ggTCAACAGCCCCAAGGTGAGGATTCGTGTAATGGTCGCGGTATCCAGGCCCAGCCGCCCATAAAGGCGGTAGCGCAAGGCAACCCCGCCTACCCAGGTAGTGAAATTGAGGTTAAAGGCGTAGCACACGAACGCGACGGGCAATACCt                                                                                                           >  4:289692/1‑149 (MQ=255)
                                           aaCAGCCCCAAGGTGAGGATTCGTGTAATGGTCGCGGTATCCAGGCCCAGCCGCCCATAAAGGCGGTAGCGCAAGGCAACCCCGCCTACCCAGGTAGTGAAATTGAGGTTAAAGGCGTAGCACACGAACGCGACGGGCAATACCTGGc                                                                                                        <  3:28063/148‑1 (MQ=255)
                                           aaCAGCCACAAGGTGAGGATTCGTGTAATGGTCGCGGTATCCAGGCCCAGCCGCCCATAAAGGCGGTAGCGCAAGGCAACCCCGCCTACCCAGGTAGTGAAATTGAGGTTAAAGGCGTAGCACACGAACGCGACGGGCAATACCTGGCg                                                                                                       >  2:184215/1‑149 (MQ=255)
                                            aCAGCCCCAAGGTGAGGATTCGTGTAATGGTCGCGGTATCCAGGCCCAGCCGCCCATAAAGGCGGTAGCGCAAGGCAACCCCGCCTACCCAGGTAGTGAAATTGAGGTTAAAGGCGTAGCACACGAACGCGACGGGCAATACCTGGCgg                                                                                                      <  7:267488/149‑1 (MQ=255)
                                                       gTGAGGATTCGTGTAATGGTCGCGGTATCCAGGCCCAGCCGCCCATAAAGGCGGTAGCGCAAGGCAACCCCGCCTACCCAGGTAGTGAAATTGAGGTTAAAGGCGTAGCACACGAACGCGACGGGCAATACCTGGCGGGCAGGTAGCg                                                                                            >  5:318040/1‑148 (MQ=255)
                                                            gATTCGTGTAATGGTCGCGGTATCCAGGCCCAGCCGCCCATAAAGGCGGTAGCGCAAGGCAACCCCGCCTACCCAGGTAGTGAAATTGAGGTTAAAGGCGTAGCACACGAACGCGACGGGCAATACCTGGCGGGCAGGTAGCGTATGGc                                                                                      >  6:270653/1‑149 (MQ=255)
                                                              ttCGTGTAATGGTCGCGGTATCCAGGCCCAGCCGCCCATAAAGGCGGTAGCGCAAGGCAACCCCGCCTACCCAGGTAGTGAAATTGAGGTTAAAGGCGTAGCACACGAACGCGACGGGCAATACCTGGCGGGCAGGTAGCGTATGGCCg                                                                                    <  6:318040/149‑1 (MQ=255)
                                                              ttCGTGTAATGGTCGCGGTATCCAGGCCCAGCCGCCCATAAAGGCGGTAGCGCAAGGCAACCCCGCCTACCCAGGTAGTGAAATTGAGGTTAAAGGCGTAGCACACGAACGCGACGGGCAATACCTGGCGGGCAGGTAGCGTATGGCCg                                                                                    >  1:162987/1‑149 (MQ=255)
                                                                 gtgtAATGGTCGCGGTATCCAGGCCCAGCCGCCCATAAAGGCGGTAGCGCAAGGCAACCCCGCCTACCCAGGTAGTGAAATTGAGGTTAAAGGCGTAGCACACGAACGCGACGGGCAATACCTGGCGGGCATGTAGCGTATGGCCGgt                                                                                  <  7:299419/148‑1 (MQ=255)
                                                                 gtgtAATGGTCGCGGTATCCAGGCCCAGCCGCCCATAAAGGCGGTAGCGCAAGGCAACCCCGCCTACCCAGGTAGTGAAATTGAGGTTAAAGGCGTAGCACACGAACGCGACGGGCAATACCTGGCGGGCAGGTAGCGTATGGCCGgtg                                                                                 >  4:252710/1‑149 (MQ=255)
                                                                    tAATGGTCGCGGTATCCAGGCCCAGCCGCCCATAAAGGCGGTAGCGCAAGGCAACCCCGCCTACCCAGGTAGTGAAATTGAGGTTAAAGGCGTAGCACACGAACGCGACGGGCAATACCTGGCGGGCAGGTAGCGTATGGCCGGTGTaa                                                                              <  5:230460/149‑1 (MQ=255)
                                                                          tCGCGGTATCCAGGCCCAGCCGCCCATAAAGGCGGTAGCGCAAGGCAACCCCGCCTACCCAGGTTGTGAAATTGAGGTTAAAGGCGTAGCACACGAACGCGACGGGCAATACCTGGCg                                                                                                       >  3:285233/1‑118 (MQ=255)
                                                                          tCGCGGTATCCAGGCCCAGCCGCCCATAAAGGCGGTAGCGCAAGGCAACCCCGCCTACCCAGGTAGTGAAATTGAGGTTAAAGGCGTAGCACACGAACGCGACGGGCAATACCTGGCg                                                                                                       <  4:285233/118‑1 (MQ=255)
                                                                          tCGCGGTATCCAGGCCCAGCCGCCCATAAAGGCGGTAGCGCAAGGCAACCCCGCCTACCCAGGTAGTGAAATTGAGGTTAAAGGCGTAGCACACGAACGCGACGGGCAATACCTGGCGGGCAGGTAGCGTATGGCCGGTGTAAGCCCgg                                                                        <  8:15073/149‑1 (MQ=255)
                                                                            gcgGTATCCAGGCCCAGCCGCCCATAAAGGCGGTAGCGCAAGGCAACCCCGCCTACCCAGGTAGTGAAATTGAGGTTAAAGGCGTAGCACACGAACGCGACGGGCAATACCTGGCGGGCAGGTAGCGTATGGCCg                                                                                    <  6:198489/135‑1 (MQ=255)
                                                                            gcgGTATCCAGGCCCAGCCGCCCATAAAGGCGGTAGCGCAAGGCAACCCCGCCTACCCAGGTAGTGAAATTGAGGTTAAAGGCGTAGCACACGAACGCGACGGGCAATACCTGGCGGGCAGGTAGCGTATGGCCg                                                                                    >  5:198489/1‑135 (MQ=255)
                                                                               gTATCCAGGCCCAGCCGCCAATAAAGGCGGTAGCGCAAGGCAACCCCGCCTACCCAGGTAGTGAAATTGAGGTTAAAGGCGTAGCACACGAACGCGACGGGCAATACCTGGCGGGCAGGTAGCGTATGGCCGGTGTAAGCCCGGGCCaa                                                                   >  3:203457/1‑149 (MQ=255)
                                                                                  tCCAGGCCCAGCCGCCCATAAAGGCGGTAGCGCAAGGCAACCCCGCCTACCCAGGTAGTGAAATTGAGGTTAAAGGCGTAGCACACGAACGCGACGGGCAATACCTGGCGGGCAGGTAGCGTATGGCCGGTGTAAGCCCGGGCCAACa                                                                 <  7:113414/148‑1 (MQ=255)
                                                                                  tCCAGGCCCAGCCGCCCATAAAGGCGGTAGCGCAAGGCAACCCCGCCTACCCAGGTAGTGAAATTGAGGTTAAAGGCGTAGCACACGAACGCGACAGGCAATACCTGGCGGGCAGGTAGCGTATGGCCGGTGTAAGCCCGGGCCAACAg                                                                >  2:89626/1‑149 (MQ=255)
                                                                                   ccAGGCCCAGCCGCCCATAAAGGCGGTAGCGCAAGGCAACCCCGCCTACCCAGGTAGTGAAATTGAGGTTAAAGGCGTAGCACACGAACGCGACGGGCAATACCTGGCGGGCAGGTAGCGTATGGCCGGTGTAAGCCCGGGCCAACAgg                                                               >  1:96103/1‑149 (MQ=255)
                                                                                          cAGCCGCCCATAAAGGCGGTAGCGCAAGGCAACCCCGCCTACCCAGGTAGTGAAATTGAGGTTAAAGGCGTAGCACACGAACGCGACGGGCAATACCTGGCGGGCAGGTAGCGt                                                                                           <  4:298104/114‑1 (MQ=255)
                                                                                          cAGCCGCCCATAAAGGCGGTAGCGCAAGGCAACCCCGCCTACCCAGGTAGTGAAATTGAGGTTAAAGGCGTAGCACACGAACGCGACGGGCAATACCTGGCGGGCAGGTAGCGt                                                                                           >  3:298104/1‑114 (MQ=255)
                                                                                           aGCCGCCCATAAAGGCGGTAGCGCAAGGCAACCCCGCCTACCCAGGTAGTGAAATTGAGGTTAAAGGCGTAGCACACGAACGCGACGGGCAATACCTGGCGGGCAGGTAGCGTATGGCCGGTGTAAGCCCGGGCCAACAGGTCGTAACt                                                       >  2:183239/1‑149 (MQ=255)
                                                                                                 ccATAAAGGCGGTAGCGCAAGGCAACCCCGCCTACCCAGGTAGTGAAATTGAGGTTAAAGGCGTAGCACACGAACGCGACGGGCAATACCTGGCGGGCAGGTAGCGTATGGCCGGTGTAAGCCCGGGCCAACAGGTCGTAACTGGCaaa                                                 >  4:232787/1‑149 (MQ=255)
                                                                                                      aaGGCGGTAGCGCAAGGCAACCCCGCCTACCCAGGTAGTGAAATTGAGGTTAAAGGCGTAGCACACGAACGCGACGGGCAATACCTGGCGGGCAGGTAGCGTATGGCCGGTGTAAGCCCGGGCCAACAGGTCGTAACTGGCAAACGTGa                                            <  3:68326/149‑1 (MQ=255)
                                                                                                         gCGGTAGCGCAAGGCAACCCCGCCTACCCAGGTAGTGAAATTGAGGTTAAAGGCGTAGCACACGAACGCGACGGGCAATACCTGGCGGGCAGGTAGCGTATGGCCGGTGTAAGCCCGGGCCAACAGGTCGTAACTGGCAAACGTGAAg                                          >  8:199656/1‑148 (MQ=255)
                                                                                                             tAGCGCAAGGCAACCCCGCCTCCCCAGGTAGTGAAATCGAGGTTAAAGGCGTAGCACACGAACGCGACGGGCAATACCTGGCGGGCAGGTAGCGTATGGCCGGTGTAAGCCCGGGCCAACAGGTCGTAACTGGCAAACGTGAAGTAGCt                                     >  3:25937/1‑149 (MQ=255)
                                                                                                             tAGCGCAAGGCAACCCCGCCTACCCAGGTAGTGAAATTGAGGTTAAAGGCGTAGCACACGAACGCGACGGGCAATACCTGGCGGGCAGGTAGCGTATGGCCGGTGTAAGCCCGGGCCAACAGGTCGTAACTGGCAAACGTGAAGTAGCt                                     >  6:153021/1‑149 (MQ=255)
                                                                                                             tAGCGCAAGGCAACCCCGCCTACCCAGGTAGTGAAATTGAGGTTAAAGGCGTAGCACACGAACGCGACGGGCAATACCTGGCGGGCAGGTAGCGTATGGCCGGTGTAAGCCCGGGCCAACAGGTCGTAACTGGCAAACGTGAAGTAGCt                                     <  3:252710/149‑1 (MQ=255)
                                                                                                             tAGCGCAAGGCAACCCCGCCTACCCAGGTAGTGAAATTGAGGTTAAAGGCGTAGCACACGAACGCGACGGGCAATACCTGGCGGGCAGGTAGCGTATGGCCGGTGTAAGCCCGGGCCAACAGGTCGTAACTGGCAAACGTGAAGTAGCt                                     <  2:162987/149‑1 (MQ=255)
                                                                                                             tAGCGCAAGGCAACCCCGCCTACCCAGGTAGTGAAATCGAGGTTAAAGGCGTAGCACACGAACGCGACGGGCAATACCTGGCGGGCAGGTAGCGTATGGCCGGTGTAAGCCCGGGCCAACAGGTCGTAACTGGCAAACGTGAAGTAGCt                                     >  3:25939/1‑149 (MQ=255)
                                                                                                                 gcAAGGCAACCCCGCCTACCCAGGTAGTGAAATTGAGGTTAAAGGCGTAGCACACGAACGCGACGGGCAATACCTGGCGGGCAGGTAGCGTATGGCCGGTGTAAGCCCGGGCCAACAGGTCGTAACTGGCAAACGTGAAGTAGCTGca                                  >  1:43894/1‑148 (MQ=255)
                                                                                                                          ccccGCCTACCCAGGTAGTGAAATTGAGGTTAAAGGCGTAGCACACGAACGCGACGGGCAATACCTGGCGGGCAGGTAGCGTATGGCCGGTGTAAGCCCGGGCCAACAGGTCGTAACTGGCAAACGTGAAGTAGCTGCACAACGCGATc                        >  4:113248/1‑149 (MQ=255)
                                                                                                                             cGCCTACCCAGGTAGTGAAATTGAGGTTAAAGGCGTAGCACACGAACGCGACGGGCAATACCTGGCGGGCAGGTAGCGTATGGCCGGTGTAAGCCCGGGCCAACAGGTCGTAACTGGCaaa                                                 >  5:283917/1‑121 (MQ=255)
                                                                                                                             cGCCTACCCAGGTAGTGAAATTGAGGTTAAAGGCGTAGCACACGAACGCGACGGGCAATACCTGGCGGGCAGGTAGCGTATGGCCGGTGTAAGCCCGGGCCAACAGGTCGTAACTGGCaaa                                                 <  6:283917/121‑1 (MQ=255)
                                                                                                                               ccTACCCAGGTAGTGAATTCGAGGTTAAAGGCGTATCACACGAACGCGAGGGGCAATACCTGGCGGGCAGGTAGCGTATGGCCGGTGTAAGCCCGGGCCAACAGGTCGTAACTGGCAAACGTGAAGTAGCTGCACAACGCGATCGCCaa                   <  4:25939/149‑1 (MQ=255)
                                                                                                                               ccTACCCAGGTAGTGAAATCGAGGTTAAAGGCGTAGCACACGAACGCGACGGGCAATACCTGGCGGGCAGGTAGCGTATGGCCGGTGTAAGCCCGGGCCAACAGGTCGTAACTGGCAAACGTGAAGTAGCTGCACAACGCGATCGCCaa                   <  4:25937/149‑1 (MQ=255)
                                                                                                                                   cccAGGTAGTGAAATTGAGGTTAAAGGCGTAGCACACGAACGCGACGGGCAATACCTGGCGGGCAGGTAGCGTATGGCCGGTGTAAGCCCGGGCCAACAGGTCGTAACTGGCAAACGTGAAGTAGCTGCACAACGCGATCGCCAAGcc                >  1:199716/1‑148 (MQ=255)
                                                                                                                                     cAGGTAGTGAAATTGAGGTTAAAGGCGTAGCACACGAACGCGACGGGCAATACCTGGCGGGCAGGTAGCGTATGGCCGGTGTAAGCCCGGGCCAACAGGTCGTAACTGGCAAACGTGAAGTAGCTGCACAACGCGATCGCCAAGCCGAt             <  6:208795/149‑1 (MQ=255)
                                                                                                                                      aGGTAGTTAAATTGAGGTTAAAGGCGTAGCACACTAACGCGACGGGCAATACCTGGCGGGCAGGTAGCGTATGGCCGGTGTAAGCCCGGGCCAACAGGTCGTAACTGGCAAACGTGAAGTAGCTGCACAACGCGATCGCCAAGCCGATg            <  1:184215/149‑1 (MQ=255)
                                                                                                                                            tGAAATTGAGGTTAAAGGCGTAGCACACGAACGCGACGGGCAATACCTGGCGGGCAGGTAGCGTATGGCCGGTGTAAGCCCGGGCCAACAGGTCGTAACTGGCAAACGTGAAGTAGCTGCACAACGCGATCGCCAAGCCGATGGCAAgg      >  5:108841/1‑149 (MQ=255)
                                                                                                                                            tGAAATTGAGGTTAAAGGCGTAGCACACGAACGCGACGGGCAATACCTGGCGGGCAGGTAGCGTATGGCCGGTGTAAGCCCGGGCCAACAGGTCGTAACTGGCAAACGTGAAGTAGCTGCACAACGCGATCGCCAAGCCGATGGCAAgg      <  2:43894/149‑1 (MQ=255)
                                                                                                                                            tGAAATTGAGGTTAAAGGCGTAGCACACGAACGCGACGGGCAATACCTGGCGGGCAGGTAGCGTATGGCCGGTGTAAGCCCGGGCCAACAGGTCGTAACTGGCAAACGTGAAGTAGCTGCACAACGCGATCGCCAAGCCGATGGCAAgg      >  6:327442/1‑149 (MQ=255)
                                                                                                                                            tGAAATTGAGGTTAAAGGCGTAGCACACGAACGCGACGGGCAATACCTGGCGGGCAGGTAGCGTATGGCCGGTGTAAGCCCGGGCCAACAGGTCGTAACTGGCAAACGTGAAGTAGCTGCACAACGCGATCGCCAAGCCGATGGCAAgg      >  5:125627/1‑149 (MQ=255)
                                                                                                                                              aaaTTGAGGTTAAAGGCGTAGCACACGAACGCGACGGGCAATACCTGGCGGGCAGGTAGCGTATGGCCGGTGTAAGCCCGGGCCAACAGGTCGTAACTGGCAAACGTGAAGTAGCTGCACAACGCGATCGCCAAGCCGATGGCAAGGGt    <  3:113248/149‑1 (MQ=255)
                                                                                                                                                 ttGAGGTTAAAGGCGTAGCACACGAACGCGACGGGCAATACCTGGCGGGCAGGTAGCGTATGGCCGGTGTAAGCCCGGGCCAACAGGTCGTAACTGGCAAACGTGAAGTAGCTGCACAACGCGATCGCCAAGCCGATGGCAAGGGTGc  >  3:192/1‑148 (MQ=255)
                                                                                                                                                  |                                                                                                                                                  
AAATACGGTGCCGGCCAGCAGCATATAGCCCATCCAGTTGGTCAACAGCCCCAAGGTGAGGATTCGTGTAATGGTCGCGGTATCCAGGCCCAGCCGCCCATAAAGGCGGTAGCGCAAGGCAACCCCGCCTACCCAGGTAGTGAAATTGAGGTTAAAGGCGTAGCACACGAACGCGACGGGCAATACCTGGCGGGCAGGTAGCGTATGGCCGGTGTAAGCCCGGGCCAACAGGTCGTAACTGGCAAACGTGAAGTAGCTGCACAACGCGATCGCCAAGCCGATGGCAAGGGTGC  >  NC_002947/3698036‑3698328

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑