| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 3,733,263 | T→A | 8.2% | intergenic (‑23/‑49) | PP_3299 ← / → PP_3300 | lipoprotein/Transcriptional regulator, tetR family |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 3,733,263 | 0 | T | A | 8.2% | 177.5 / 5.7 | 61 | intergenic (‑23/‑49) | PP_3299/PP_3300 | lipoprotein/Transcriptional regulator, tetR family |
| Reads supporting (aligned to +/- strand): ref base T (28/28); new base A (3/2); total (31/30) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.63e-01 | |||||||||||
CGACGTAATGATCGGCCAGCGCAACCTCTGGCTGTTTATAATCCGGGCCAACCATGCACCCTGCAGTGAGACTGGCGCTCAACAGCAGAGCAATCGTTCGGTAGGGAAGCATGATGATTCTCATAGACCAAGAGGAGGTGTGACCATATTATATTTTGGTCACACTCTGTCAATCAGTTCCAGGATGCTTCAAAAATGAGTAAAT‑CTTCTCCACCCTCGCCGGCGCGCGGGCCAGCCGACCACGACGTGAGGGATCAGATCATCCAGGCTGCCATGGAGCACTTCGCCCATTA > NC_002947/3733117‑3733409 | cgACGTAATGATCGGCCAGCGCAACCTCTGGCTGTTTATAATCCGGGCCAACCATGCACCCTGCAGTGAGACTGGCGCTCAACAGCAGAGCAATCGTTCGGTAGGGAAGCATGATGATTCTCATAGACCAAGAGGAGGTGTGACCatat > 2:306474/1‑149 (MQ=255) gTAATGATCGGCCAGCGCAACCTCTGGCTGTTTATAATCCGGGCCAACCATGCACCCTGCAGTGAGACTGGCGCTCAACAGCAGAGCAATCGTTCGGTAGGGAAGCATGATGATTCTCATAGACCAAGAGGAGGTGTGACCatattata < 1:326665/149‑1 (MQ=255) ggCCAGCGCAACCTCTGGCTGTTTATAATCCGGGCCAACCATGCACCCTGCAGTGAGACTGGCGCTCAACAGCAGAGCAATCGTTCGGTAGGGAAGCATGATGATTCTCATAGACCAAGAGGAGGTGTGACCATATTATATTTTGGTca > 5:200633/1‑149 (MQ=255) ccAGCGCAACCTCTGGCTGTTTATAATCCGGGCCAACCATGCACCCTGCAGTGAGACTGACGCTCAACAGCAGAGCAATCGTTCGGTAGGGAAGCATGATGATTCTCATAGACCAAGAGGAGGTGTGACCAAAATATAATATGGTcaca < 2:86488/149‑1 (MQ=255) cAACCTCTGGCTGTTTATAATCCGGGCCAACCATGCACCCTGCAGTGAGACTGGCGCTCAACAGCAGAGCAATCGTTCGGTAGGGAAGCATGATGATTCTCATAGACCAAGAGGAGGTGTGACCATATTATATTTTGGTCACACTCTGt < 1:306474/149‑1 (MQ=255) aaCCTCTGGCTGTTTATAATCCGAGCCAACCATGCACCCTGCAGTGAGACTGGCGCTCAACAGCAGAGCAATCGTTCGGTAGGGAAGCATGATGATTCTCATAGACCAAGAGGAGGTGTGACCATATTATATTTTGGTCACACTCTGt > 1:63635/1‑148 (MQ=255) aCCTCTGGCTGTTTATAATCCGGGCCAACCATGCACCCTGCAGTGAGACTGGCGCTCAACAGCAGAGCAATCGTTCGGTAGGGAAGCATGATGATTCTCATAGACCAAGAGGAGGTGTGACCATATTATATTTTGGTCACACTCTGTc > 4:109262/1‑148 (MQ=255) ctctGGCTGTTTATAATCCGGGCCAACCATGCACCCTGCAGTGAGACTGGCGCTCAACAGCAGAGCAATCGTTCGGTAGGGAAGCATGATGATTCTCATAGACCAAGAGGAGGTGTGACCATATTATATTTTGGTCACACTCTGTCAAt < 8:117573/149‑1 (MQ=255) ctctGGCTGTTTATAATCCGGGCCAACCATGCACCCTGCAGTGAGACTGGCGCTCAACAGCAGAGCAATCGTTCGGTAGGGAAGCATGATGATTCTCATAGACCAAGAGGAGGTGTGACCATATTATATTTTGGTCACACTCTGTCAAt < 3:153505/149‑1 (MQ=255) ctctGGCTGTTTATAATCCGGGCCAACCATGCACCCTGCAGTGAGACTGGCGCTCAACAGCAGAGCAATCGCTCGGTAGGGAAGCATGATGATTCTCATAGACCAAGAGGAGGTGTGACCATATTATATTTTGGTCACACTCTGTCAAt < 2:101749/149‑1 (MQ=255) tGGCTGTTTATAATCCGGCCCAACCATGCACCCTGCAGTGAGACTGTCGCTCAACATCAGAGCAATCGTTCGGTAGGGAAGCATGATGATTCTCATAGACCAAGAGGAGGTGTGACCATATTATATTTTGGTCACACTCTGTCAATCa < 5:343092/148‑1 (MQ=255) tttATAATCCGGGCCAACCATGCACCCTGCAGTGAGACTGGCGCTCAACAGCAGAGCAATCGTTCGGTAGGGAAGCATGATGATTCTCATAGACCAAGAGGAGGTGTGACCATATTATATTTTGGTCACActct < 5:251884/134‑1 (MQ=255) tttATAATCCGGGCCAACCATGCACCCTGCAGTGAGACTGGCGCTCAACAGCAGAGCAATCGTTCGGTAGGGAAGCATGATGATTCTCATAGACCAAGAGGAGGTGTGACCATATTATATTTTGGTCACActct > 6:251884/1‑134 (MQ=255) tacGGGCCAACCATGCACCCTGCAGTGAGACTGGCGCTCAACAGCAGAGCAATCGTTCGGTAGGGAAGCATGATGATTCTCATAGACCAAGAGGAGGTGTGACCATATTATATTTTGGTCACACTCTGTCAATCAGTTCCAGGATGCtt < 8:112347/147‑1 (MQ=255) tCCGGGCCAACCATGCACCCTGCAGTGAGACTGGCGCTCAACAGCAGAGCAATCGTTCGGTAGGGAAGCATGATGATTCTCATAGACCAAGAGGAGGTGTGACCATATTATATTTAGGTCACACTCTGTCAATCAGTTCCAGGATGCtt > 3:299780/1‑149 (MQ=255) cgtgCCAACCATGCACCCTGCAGTGAGACTGGCGCTCAACAGCAGAGCAATCGTTCGGTAGGGAAGCATGATGATTCTCATAGACCAAGAGGAGGTGTGACCATATTATATTTTGGTCACACTCTGTCAATCAGTTCCAGGATGCTTCa < 5:115232/146‑1 (MQ=255) ccAACCATGCACCCTGCAGTGAGACTGGCGCTCAACAGCAGAGCAATCGTTCGGTAGGGAAGCATGATGATTCTCATAGACCAAGAGGAGGTGTGACCATATTATATTTTGGTCACACTCTGTCAATCAGTTCCa < 6:187361/135‑1 (MQ=255) ccAACCATGCACCCTGCAGTGAGACTGGCGCTCAACAGCAGAGCAATCGTTCGGTAGGGAAGCATGATGATTCTCATAGACCAAGAGGAGGTGTGACCATATTATATTTTGGTCACACTCTGTCAATCAGTTCCa > 5:187361/1‑135 (MQ=255) ccAACCATGCACCCTGCAGTGAGACTGGCGCTCAACAGCAGAGCAATCGTTCGGTAGGGAAGCATGATGATTCTCATAGACCAAGAGGAGGTGTGACCATATTATATTTTGGTCACACTCTGTCAATCAGTTCCAGGATGCTTCaaaaa > 4:10650/1‑149 (MQ=255) aaCCATGCACCCTGCAGTGAGACTGGCGCTCAACAGCAGAGCAATCGTTCGGTAGGGAAGCATGATGATTCTCATAGACCAAGAGGAGGTGTGACCATATTATATTTTGGTCACACTCTGTCAATCAGTTCCAGGATGCTTCAAAAAt > 6:70650/1‑148 (MQ=255) aaCCATGCACCCTGCAGTGAGACTGGCGCTCAACAGCAGAGCAATCGTTCGGTAGGGAAGCATGATGATTCTCATAGACCAAGAGGAGGTGTGACCATATTATATTTTGGTCACACTCTGTCAATCAGTTCCAGGATGCTTCAAAAAt > 6:15492/1‑148 (MQ=255) aCCATGCACCCTGCAGTGAGACTGGCGCTCAACAGCAGAGCAATCGTTCGGTAGGGAAGCATGATGATTCTCATAGACCAAGAGGAGGTGTGACCATATTATATTTTGGTCACACTCTGTCAATCAGTTCCGGGAt > 1:243032/1‑136 (MQ=255) aCCATGCACCCTGCAGTGAGACTGGCGCTCAACAGCAGAGCAATCGTTCGGTAGGGAAGCATGATGATTCTCATAGACCAAGAGGAGGTGTGACCATATTATATTTTGGTCACACTCTGTCAATCAGTTCCGGGAt < 2:243032/136‑1 (MQ=255) aCCATGCACCCTGCAGTGAGACTGGCGCTCAACAGCAGAGCAATCGTTCGGTAGGGAAGCATGATGATTCTCATAGACCAAGAGGAGGTGTGACCATATTATATTTTGGTCACACTCTGTCAATCAGTTCCAGGATGCTTCAAAAATGa < 3:109262/149‑1 (MQ=255) cATGCACCCTGCAGTGAGACTGGCGCTCAACAGCAGAGCAATCGTTCGGTAGGGAAGCATGATGATTCTCATAGACCAAGAGGAGGTGTGACCATATTATATTTTGGTCACACTCTGTCAATCAGTTCCAGGATGCTTCAAAAATGAGt < 5:70650/149‑1 (MQ=255) tGCACCCTGCAGTGAGACTGGCGCTCAACAGCAGAGCAATCGTTCGGTAGGGAAGCATGATGATTCTCATAGACCAAGAGGAGGTGTGACCATATTATATTTTGGTCACACTCTGTCAATCAGTTCCAGGATGCTTCTAAAATGAGTa > 7:125660/1‑148 (MQ=255) ccTGCAGTGAGACTGGCGCTCAACAGCAGAGCAATCGTTCGGTAGGGAAGCATGATGATTCTCATAGACCAAGAGGAGGTGTGACCATATTATATTTTGGTca > 4:75979/1‑103 (MQ=255) ccTGCAGTGAGACTGGCGCTCAACAGCAGAGCAATCGTTCGGTAGGGAAGCATGATGATTCTCATAGACCAAGAGGAGGTGTGACCATATTATATTTTGGTca < 3:75979/103‑1 (MQ=255) cTGCAGTGAGACTGGCGCTCAACAGCAGAGCAATCGTTCGGTAGGGAAGCATGATGATTCTCATAGACCAAGAGGAGGTGTGACCATATTATATTTTGGTCACACTCTGTCAATCAGTTCCAGGATGCTTCAAAAATGAGTa > 5:221157/1‑142 (MQ=255) cTGCAGTGAGACTGGCGCTCAACAGCAGAGCAATCGTTCGGTAGGGAAGCATGATGATTCTCATAGACCAAGAGGAGGTGTGACCATATTATATTTTGGTCACACTCTGTCAATCAGTTCCAGGATGCTTCAAAAATGAGTa < 6:221157/142‑1 (MQ=255) cAGTGAGACTGGCGCTCAACAGCAGAGCAATCGTTCGGTAGGGAAGCATGATGATTCTCATAGACCAAGAGGAGGTGTGACCATATTATATTTTGGTCACACTCTGTCAATCAGTTCCAGGATGCTTCAAAAATGAGTAAAT‑CTTCTcc < 3:6438/149‑1 (MQ=255) cAGTGAGACTGGCGCTCAACAGCAGAGCAATCGTTCGGTAGGGAAGCATGATGATTCTCATAGACCAAGAGGAGGTGTGACCATATTATATTTAGGTCACACTCTGTCAATCAGTTCCAGGATGCTTCAAAAATGAGTAAAT‑CTTCTcc < 4:299780/149‑1 (MQ=255) gcTCAACAGCAGAGCAATCGTTCGGTAGGGAAGCATGATGATTCTCATAGACCAAGAGGAGGTGTGACCATATTATATTTTGGTCACACTCTGTCAATCAGTTCCAGGATGCTTCAAAAATGAGTAAAT‑CTTCTCCACCCTCGCCGgcg > 5:239298/1‑149 (MQ=255) tCAACAGCAGAGCAATCGTTCGGTAGGGAAGCATGATGATTCTCATAGACCAAGAGGAGGTGTGACCATATTATATTTTGGTCACACTCTGTCAATCAGTTCCAGGATGCTTCAAAAATGAGTAAAT‑CTTCGCCACCCTCGCCGgcgcg > 6:82364/1‑149 (MQ=255) aaCAGCAGAGCAATCGTTCGGTAGGGAAGCATGATGATTCTCATAGACCAAGAGGAGGTGTGACCATATTATATTTTGGTCACACTCTGTCAATCAGTTCCAGGATGCTTCAAAAATGAGTAAAT‑CTTCTCCACCCTCGCCGGcgcgcg > 8:160765/1‑149 (MQ=255) aaCAGCAGAGCAATCGTTCGGTAGGGAAGCATGATGATTCTCATAGACCAAGAGGAGGTGTGACCATATTATATTTTGGTCACACTCTGTCAATCAGTTCCAGGATGCTTCAAAAATGAGTAAAT‑CTTCTCCACCCTCGCCGGcgcgcg < 6:239298/149‑1 (MQ=255) gcagAGCAATCGTTCGGTAGGGAAGCATGATGATTCTCATAGACCAAGAGGAGGTGTGACCATATTATATTTTGGTCACACTCTGTCAAt < 8:136008/90‑1 (MQ=255) gcagAGCAATCGTTCGGTAGGGAAGCATGATGATTCTCATAGACCAAGAGGAGGTGTGACCATATTATATTTTGGTCACACTCTGTCAAt > 7:136008/1‑90 (MQ=255) tCGTTCGGTAGGGAAGCATGATGATTCTCATAGACCAAGAGGAGGTGTGACCATATTATATTTTGGTCACACTCTGTCAATCAGTTCCAGGATGCTTCAAAAATGAGTAAAT‑CTTCTCCACCATCGCCGGCGCGCGGGCCAGCCGACCa > 3:66314/1‑149 (MQ=255) cGTTCGGTAGGGAAGCATGATGATTCTCATAGACCAAGAGGAGGTGTGACCATATTATATTTTGGTCACACTCTGTCAATCAGTTCCAGGATGCTTCAAAAATGAGTAAAT‑CTTCTCCACCCTCGCCGGCGCGCGGGCCAGCCGACCac > 5:288498/1‑149 (MQ=255) gTATGGAATCATTATGAATCTCATAGAACAATACGATGTGTGACCATATTATATTTTGGTCAAAATCTGTCAATCATTTCCAGGATGCTTCAAAAATGATTAAATACTTCTCCACCCTCGCCGGCGCGCGGGCCAGCCGACCACGACGt < 1:65763/149‑1 (MQ=255) gggAAGCATGATGATTCTCATAGACCAAGAGGAGGTGTGACCATATTATATTTTGGTCACACTCTGTCAATCAGTTCCAGGATGCTTCAAAAATGAGTAAAT‑CTTCTCCACCCTCGCCGGCGCGCGGGCCAGCCGACCACGACGTGAgg < 3:10650/149‑1 (MQ=255) aGCATGATGATTCTCATAGACCAAGAGGAGGTGTGACCATATTATATTTTGGTCACACTCTGTCAATCAGTTCCAGGATGCTTCAAAAATGAGTAAAT‑CTTCTCCACCCTCGCCGGCGCGCGGGCCAGCCGACCACGACGTGAGGgatc < 6:224363/149‑1 (MQ=255) atgatgATTCTCATAGACCAAGAGGAGGTGTGACCATATTATATTTTGGTCACACTCTGTCAATCAGTTCCAGGATGCTTCAAAAATGAGTAAAT‑CTTCTCCACCCTCGCCGGCGCGCGGGCCAGCCGACCACGACGTGAGGgatcaga < 5:15492/149‑1 (MQ=255) tgatTCTCATAGACCAAGAGGAGGTGTGACCATATTATATTTTGGTCACACTCTGTCAATCAGTTCCAGGATGCTTCAAAAATGAGTAAAT‑CTTCTCCACCCTCGCCGGCGCGCGGGCCAGCCGACCACGACGTGAGGgatcagatca < 6:200633/148‑1 (MQ=255) gatTCTCATAGACCAAGAGGAGGTGTGACCATATTATATTTTGGTCACACTCTGTCAATCAGTTCCAGGATGCTTCAAAAATGAGTAAAT‑CTTCTCCACCCTCGCCGGCGCGCGGGCCAGCCGa > 4:24797/1‑124 (MQ=255) gatTCTCATAGACCAAGAGGAGGTGTGACCATATTATATTTTGGTCACACTCTGTCAATCAGTTCCAGGATGCTTCAAAAATGAGTAAAT‑CTTCTCCACCCTCGCCGGCGCGCGGGCCAGCCGACCACGACGTGAGGGATCAGatcatc > 6:319761/1‑149 (MQ=255) ttCTCATAGACCAAGAGGAGGTGTGACCATATTATATTTTGGTCACACTCTGTCAATCAGTTCCAGGATGCTTCAAAAATGAGTAAAT‑CTTCTCCACCCTCGCCGGCGCGCGGGCCAGCCGACCACGACGTGAGGGATCAGatcatc > 2:323770/1‑147 (MQ=255) tcATAGACCAAGAGGAGGTGTGACCATATTATATTTTGGTCACACTCTGTCAATCAGTTCCAGGATGCTTCAAAAATGAGTAAAT‑CTTCTCCACCCTCGCCGGCGCGCGGGCCAGCCGACCACGACGTGAGGGATCAGATCATCCAGGc > 7:13590/1‑149 (MQ=255) aTAGACCAAGAGGAGGTGTGACCATATTATATTTTGGTCACACTCTGTCAATCAGTTCCAGGATGCTTCAAAAATGAGTAAAT‑CTTCTCCACCCTCGCCGGCGCGCGGGCCAGCCGACCACGACGTGAGGGATCAGATCATCCAGGCTg > 5:251459/1‑149 (MQ=255) aTAGACCAAGAGGAGGTGTGACCATATTATATTTTGGTCACACTCTGTCAATCAGTTCCAGGATGCTTCAAAAATGAGTAAAT‑CTTCTCCACCCTCGCCGGCGCGCGGGCCAGCCGACCACGACGTGAGGGATCAGATCATCCAGGCTg > 6:179850/1‑149 (MQ=255) gACCAAGAGGAGGTGTGACCATATTATATTTTGGTCACACTCTGTCAATCAGTTCCAGGATGCTTCAAAAATGAGTAAAT‑CTTCTCCACCCTCGCCGGCGCGCGGGCCAGCCGACCACGACGTGAGGGATCAGATCATCCAGGCTGCCa > 8:118811/1‑149 (MQ=255) aaGAGGAGGTGTGACCATATTATATTTTGGTCACACTCTGTCAATCAGTTCCAGGATTCTTCAAAAATGAGTAAAT‑CTTCTCCACCCTCGCCGGCGCGCGGGCCAGCCGACCACGACGTGAGGGATCAGATCATCCAGGCTGCCATGGa < 6:251459/149‑1 (MQ=255) gaggagGTGTGACCATATTATATTTTGGTCACACTCTGTCAATCAGTTCCAGGATGCTTCAAAAATGAGTAAAT‑CTTCTCCACCCTCGCCGGCGCGCGGGCCAGCCGACCACGACGTGAGGGATCAGATCATCCAGGCTGCCATGGAGc < 2:63635/149‑1 (MQ=255) agtgtGACCAAAATATAATATGGTCACACTCTGTCAATCAGTTCCAGGATGCTTCAAAAATGAGTAAAT‑CTTCTCCACACTCGCCGGCGCGCGGGCCAGCCGACCACGACGTGAGGGATCAGATCATCAAGGCTCCCATGCAGCACtac > 8:258100/2‑147 (MQ=255) gtgtGACCATATTATATTTTGGTCACACTCTGTCAATCAGTTCCAGGATGCTTCAAAAATGAGTAAAT‑CTtctc < 3:5245/74‑1 (MQ=255) gtgtGACCATATTATATTTTGGTCACACTCTGTCAATCAGTTCCAGGATGCTTCAAAAATGAGTAAAT‑CTtctc > 4:5245/1‑74 (MQ=255) gtgtGACCAAAATATAATATGGTCACACTCTGTCAATCAGTTCCAGGATGCTTCAAAAATGAGTAAAT‑CTTCTCCACCCTCGCCGGCGCGCGGGCCAGCCGACCACGACGTGAGGGATCAGATCATCCAGGc > 7:172936/1‑132 (MQ=255) gtgtGACCAAAATATAATATGGTCACACTCTGTCAATCAGTTCCAGGATGCTTCAAAAATGAGTAAAT‑CTTCTCCACCCTCGCCGGCGCGCGGGCCAGCCGACCACGACGTGAGGGATCAGATCATCCAGGc < 8:172936/132‑1 (MQ=255) tgtgACCAAAATATAATATGGTCACACTCTGTCAATCAGTTCCAGGATGCTTCAAAAATGAGTAAAT‑CTTCTCCACCCTCGCCGGCGCGCGGGCCAGCCGACCACGACGTGAGGGATCAGATCATCCAGGTTGCCATGGAGCACTTCGc > 7:62737/1‑149 (MQ=255) aCCATATTATATTTTGGTCACACTCTGTCAATCAGTTCCAGGATGCTTCAAAAATGAGTAAAT‑CTTCTCCACCCTCGCCGGCGCGCGGGCCAGCCGACCACGACGTGAGGGATCAGATCATCCAGGCTGCCATGGAGCACTTCGCCCAt < 6:288498/149‑1 (MQ=255) aCCATATTATATTTTGGTCACACTCTGTCAATCAGTTCCAGGATGCTTCAAAAATGAGTAAAT‑CTTCTCCACCCTCGCCGGCGCGCGGGCCAGCCGACCACGACGTGAGGGATCAGATCATCCAGGCTGCCATGGAGCACTTCGCCCAt > 6:202317/1‑149 (MQ=255) cATATTATATTTTGGTCACACTCTGTCAATCAGTTCCAGGATGCTTCAAAAATGAGTAAAT‑CTTCTCCACCCTCGCCGGCGCGCGGGCCAGCCGACCACGACGTGAGGGATCAGATCATCCAGGCTGCCATGGAGCACTTCGCCCATTa < 1:323770/149‑1 (MQ=255) | CGACGTAATGATCGGCCAGCGCAACCTCTGGCTGTTTATAATCCGGGCCAACCATGCACCCTGCAGTGAGACTGGCGCTCAACAGCAGAGCAATCGTTCGGTAGGGAAGCATGATGATTCTCATAGACCAAGAGGAGGTGTGACCATATTATATTTTGGTCACACTCTGTCAATCAGTTCCAGGATGCTTCAAAAATGAGTAAAT‑CTTCTCCACCCTCGCCGGCGCGCGGGCCAGCCGACCACGACGTGAGGGATCAGATCATCCAGGCTGCCATGGAGCACTTCGCCCATTA > NC_002947/3733117‑3733409 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |