Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,753,598 A→C 8.5% V95V (GTT→GTG)  PP_3316 ← chaperone‑associated ATPase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,753,5980AC 8.5% 208.7 / 8.8 71V95V (GTT→GTG) PP_3316chaperone‑associated ATPase
Reads supporting (aligned to +/- strand):  ref base A (33/32);  new base C (3/3);  total (36/35)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.37e-01

GCTTGGCGGCTTCCTGCAACAGGGCCTCGGAGTGTTCGCTGATGCGGCTGGCCAGCCCGCTGCTGCGGCGGCGTGGCGTGCCGGACGCTGCGCGGCCAGGT‑TCCCCAGACGACGCTTCAGCCACTTCATCGGCATCCGTGGCAACGGATGCCGAGTCTTCTCCGATGCGCAGCAAATCGCTGCCCATGAACTCTTCGAACAAACCGCTGCGCGAGCCGAAAAGGGCTTCCAGTGGGGAAAGGGTGCGTTTTTGCTGGCGCACCAATTGGCGGTAGTGATCATCACACAACA  >  NC_002947/3753456‑3753746
                                                                                                                                               |                                                                                                                                                    
gCTTGGCGGCTTCCTGCAACAGGGCCTCGGAGTGTTCGCTGATGCGGCTGGCCAGCCCGCTGCTGCGGCGGCGTGGCGTGCCGGACGCTGCGCGGCCAGGT‑TCCCCAGACGACGCTTCAGCCACTTCATCGGCATCCGTGGCAACGGAt                                                                                                                                                >  8:187518/1‑149 (MQ=255)
  ttGGCGGCTTCCTGCAACAGGGCCTCGGAGTGTTCGCTGATGCGGCTGGCCAGCCCGCTGCTGCGGCGGCGTGGCGTGCCGGACGCTGCGCGGCCAGGT‑TCCCCAGACGACGCTTCAGCCACTTCATCGGCATCCGTGGCAACGGATGc                                                                                                                                              >  7:107266/1‑149 (MQ=255)
     gcggcTTCGTGCAACCGGGCCTGGGAGTGTTCGCTGATGCGGCTGGCCAGCCCGCTGCTGCGGCGGCGTGGCGTGCCGGACGCTGCGCGGCCAGGT‑TCCCCAGACGACGCTTCAGCCACTTCATCGGCATCCGTTGCCACGGATGCCGa                                                                                                                                           <  1:160898/149‑1 (MQ=255)
     gcggcTTCCTGCAACAGGGCCTCGGAGTGTTCGCTGATGCGGCTGGCCAGCCCGCTGCTGCGGCGGCGTGGCGTGCCGGACGCTGCGCGGCCAGGT‑TCCCCAGACGACGCTTCAGCCACTTCATCGGCATCCGTGGCAACGGATGcc                                                                                                                                             >  3:107660/1‑147 (MQ=255)
     gcggcTTCCTGCAACAGGGCCTCGGAGTGTTCGCTGATGCGGCTGGCCAGCCCGCTGCTGCGGCGGCGTGGCGTGCCGGACGCTGCGCGGCCAGGT‑TCCCCAGACGACGCTTCAGCCACTTCATCGGCATCCGTGGCAACGGATGCCGa                                                                                                                                           <  8:792/149‑1 (MQ=255)
         cTTCCTGCAACAGGGCCTCGGAGTGTTCGCTGATGCGGCTGGCCAGCCCGCTGCTGCGGCGGCGTGGCGTGCCGGACGCTGCGCGGCCAGGT‑TCCCCAGACGACGCTTCAGCCACTTCATCGGCATCCGTGGCAACGGATGCCGAGtct                                                                                                                                       >  7:301389/1‑149 (MQ=255)
         cTTCCTGCAACAGGGCCTCGGAGTGTTCGCTGATGCGGCTGGCCAGCCCGCTGCTGCGGCGGCGTGGCGTGCCGGACGCTGCGCGGCCAGGT‑TCCCCAGACGACGCTTCAGCCACTTCATCGGCATCCGTGGCAACGGATGCCGAGtct                                                                                                                                       >  5:346276/1‑149 (MQ=255)
             cTGCAACAGGGCCTCGGAGTGTTCGCTGATGCGGCTGGCCAGCCCGCTGCTGCGGCGGCGTGGCGTGCCGGACGCTGCGCGGCCAGGT‑TCCCCAGACGACGCTTCAGCCACTTCATCGGCATCCGTGGCAACGGATGCCGa                                                                                                                                           >  1:94503/1‑141 (MQ=255)
             cTGCAACAGGGCCTCGGAGTGTTCGCTGATGCGGCTGGCCAGCCCGCTGCTGCGGCGGCGTGGCGTGCCGGACGCTGCGCGGCCAGGT‑TCCCCAGACGACGCTTCAGCCACTTCATCGGCATCCGTGGCAACGGATGCCGa                                                                                                                                           <  2:94503/141‑1 (MQ=255)
              tGCAACAGGGCCTCGGAGTGTTCGCTGATGCGGCTGGCCAGCCCGCTGCTGCGGCGGCGTGGCGTGCCGGACGCTGCGCGGCCAGGT‑TCCCCAGACGACGCTTCAGCCACTTCATCGGCATCCGTTGCCACGGATGCCGa                                                                                                                                           <  7:307990/140‑1 (MQ=255)
              tGCAACAGGGCCTCGGAGTGTTCGCTGATGCGGCTGGCCAGCCCGCTGCTGCGGCGGCGTGGCGTGCCGGACGCTGCGCGGCCAGGT‑TCCCCAGACGACGCTTCAGCCACTTCATCGGCATCCGTTGCCACGGATGCCGa                                                                                                                                           >  8:307990/1‑140 (MQ=255)
                 aaCAGGGCCTCGGAGTGTTCGCTGATGCGGCTGGCCAGCCCGCTGCTGCGGCGGCGTGGCGTGCCGGACGCTGCGCGGCCAGGT‑TCCCCAGACGACGCTTCAGCCACTTCATCGGCATCCGTGGCAACGGATGCCGAGTCTTCTCCGAt                                                                                                                               <  1:4971/149‑1 (MQ=255)
                 aaCAGGGCCTCGGAGTGTTCGCTGATGCGGCTGGCCAGCCCGCTGCTGCGGCGGCGTGGCGTGCCGGACGCTGCGCGGCCAGGT‑TCCCCAGACGACGCTTCAGCCACTTCATCGGCATCCGTGGCAACGGATGCCGAGTCTTCTCCGAt                                                                                                                               >  8:162900/1‑149 (MQ=255)
                 aaCAGGGCCTCGGAGTGTTCGCTGATGCGGCTGGCCAGCCCGCTGCTGCGGCGGCGTGGCGTGCCGGACGCTGCGCGGCCAGGT‑TCCCCAGACGACGCTTCAGCCACTTCATCGGCATCCGTGGCAACGGATGCCGAGTCTTCTCCGAt                                                                                                                               <  3:57446/149‑1 (MQ=255)
                              aGTGTTCGCTGATGCGGCTGGCCAGCCCGCTGCTGCGGCGGCGTGGCGTGCCGGACGCTGCGCGGCCAGGT‑TCCCCAGACGACGCTTCAGCCACTTCATCGGCATCCGTGGCAACGGATGCCGAGTCTTCTCCGATGCGCAGCAAATCg                                                                                                                  <  3:81617/149‑1 (MQ=255)
                                tggtCGCTGAGGCGGCTGCCCAGCACGCTGCTGCGGCGGCGGGGCGTGCCGGACGCGGCGCGGCCAGGT‑TCCCCAGACGACGCTTCAGCCACTTCATCGGCATCCGTGGCAACGGATGCCGAGTCTTCTCCGATGCGCAGCAAATCGCt                                                                                                                <  4:18364/146‑1 (MQ=255)
                                  ttCGCTGTTGCGGCGGGCCAGCCCGCTGCTGCGGGGGCGTGGCGTGCGGGACGCTGCGCGGCCAGGT‑TCCCCAGACGACGCTTCAGCCACTTCATCGGCATCCGTGGCAACGGATGCCGAGTCTTCTCCGATGCGCAGCAAATCGCTg                                                                                                               <  1:171422/148‑1 (MQ=255)
                                  ttCGCTGATGCGGCTGGCCAGCCCGCTGCTGCGGCGGCGTGGCGTGCCGGACGCTGCGCGGCCAGGT‑TCCCCAGACGACGCTTCAGCCACTTCATCGGCATCCGTGGCAACGGATGCCGAGTCTTCTCCGATGCgcag                                                                                                                         <  5:266023/138‑1 (MQ=255)
                                  ttCGCTGATGCGGCTGGCCAGCCCGCTGCTGCGGCGGCGTGGCGTGCCGGACGCTGCGCGGCCAGGT‑TCCCCAGACGACGCTTCAGCCACTTCATCGGCATCCGTGGCAACGGATGCCGAGTCTTCTCCGATGCgcag                                                                                                                         >  6:266023/1‑138 (MQ=255)
                                  ttCGCTGATGCGGCTGGCCAGCCCGCTGCTGCGGCGGCGTGGCGTGCCGGACGCTGCGCGGCCAGGT‑TCCCCAGACGACGCTTCAGCCACTTCATCGGCATCCGTGGCAACGGATGCCGAGTCTTCTCCGATGCGCAGCAAATCGCTGc                                                                                                              >  1:316504/1‑149 (MQ=255)
                                  ttCGCTGATGCGGCTGGCCAGCCCGCTGCTGCGGCGGCGTGGCGTGCCGGACGCTGCGCGGCCAGGT‑TCCCCAGACGACGCTTCAGCCACTTCATCGGCATCCGTGGCAACGGATGCCGAGTCTTCTCCGATGCGCAGCAAATCGCTGc                                                                                                              >  4:121279/1‑149 (MQ=255)
                                       tGATGCGGCTGGCCAGCCCGCTGCTGCGGCGGCGTGGCGTGCCGGACGCTGCGCGGCCAGGT‑TCCCCAGACGACGCTTCAGCCACTTCATCGGCATCCGTGGCAACGGATGCCGAGTCTTCTCCGATGCgca                                                                                                                          <  1:159468/132‑1 (MQ=255)
                                       tGATGCGGCTGGCCAGCCCGCTGCTGCGGCGGCGTGGCGTGCCGGACGCTGCGCGGCCAGGT‑TCCCCAGACGACGCTTCAGCCACTTCATCGGCATCCGTGGCAACGGATGCCGAGTCTTCTCCGATGCgca                                                                                                                          >  2:159468/1‑132 (MQ=255)
                                         aTGCGGCTGGCCAGCCCGCTGCTGCGGCGGCGTGGCGTGCCGGACGCTGCGCGGCCAGGT‑TCCCCAGACGACGCTTCAGCCACTTCATCGGCATCCGTGGCAACGGATGCCGAGTCTTCTCCGATGCGCAGCAAATCGCTGCCCATgca                                                                                                       >  3:249595/1‑147 (MQ=255)
                                         aTGCGGCTGGCCAGCCCGCTGCTGCGGCGGCGTGGCGTGCCGGACGCTGCGCGGCCAGGT‑TCCCCAGACGACGCTTCAGCCACTTCATCGGCATCCGTGGCAACGGATGCCGAGTCTTCTCCGATGCGCAGCAAATCGCTGCCCATGaa                                                                                                       <  6:346276/149‑1 (MQ=255)
                                                 ggCCAGCCCGCTGCTGCGGCGGCGTGGCGTGCCGGACGCTGCGCGGCCAGGT‑TCCCCAGACGACGCTTCAGCCACTTCATCGGCATCCGTGGCAACGGATGCCGAGTCTTCTCCGATGCGCAGCAAATCGCTGCCCATGAACTCTTCg                                                                                                >  6:197852/1‑148 (MQ=255)
                                                  gCCAGCCCGCTGCTGCGGCGGCGTGGCGTGCCGGACGCTGCGCGGGCAGGT‑TCCCCAGACGACGCTTCAGCCACTTCATCGGCATCCGTGGCAACGGATGCCGAGTCTTCTCCGATGCGCAGCAAATCGCTGCCCATGAACTCTTCGaa                                                                                              >  1:241973/1‑149 (MQ=255)
                                                   ccAGCCCGCTGCTGCGGCGGCGTGGCGTGCCGGACGCTGCGCGGCCAGGT‑TCCCCAGACGACGCTTCAGCCACTTCATCGGCATCCGTGGCAACGGATGCCGAGTCTTCTCCGAt                                                                                                                               <  6:128668/115‑1 (MQ=255)
                                                    ctgCCCGGTGCTGCGGCGGCGTGGCGTGCCGTACGCTGCGCGGCCAGGT‑TCCCCAGACGACGCTTCAGCCACTTCATCGGCATCCGTGGCAACGGATGCCGAGTCTTCTCCGAt                                                                                                                               <  4:93061/112‑1 (MQ=255)
                                                    cAGCCCGCTGCTGCGGCGGCGTGGCGTGCCGGACGCTGCGCGGCCAGGT‑TCCCCAGACGACGCTTCAGCCACTTCATCGGCATCCGTGGCAACGGATGCCGAGTCTTCTCCGATGCGCAGCAAATCGCTGCCCATGAACTCTTCGAAc                                                                                             >  5:275983/1‑148 (MQ=255)
                                                          gctgctGCGGCGGCGTGGCGTGCCGGACGCTGCGCGGCCAGGT‑TCCCCAGACGACGCTTCAGCCACTTCATCGGCATCCGTGGCAACGGATGCCGAGTCTTCTCCGATGCGCAGCAAATCGCTGCCCATGAACTCTTCGAACAAACCGc                                                                                      >  3:136324/1‑149 (MQ=255)
                                                           ctgctgCGGCGGCGTGGCGTGCCGGACGCTGCGCGGCCAGGTGTCCCCAGACGACGCTTCAGCCACTTCATCGGCATCCGTGGCAACGGATGCCGAGTCTTCTCCGATGCGCAGCAAATCGCTGCCCATGAACTCTTCGAACAAACCGc                                                                                      >  1:65745/1‑149 (MQ=255)
                                                                 cggcggggTGGCGTGCCGGACGCTGCGCGGCCAGGT‑TCCCCAGACGACGCTTCAGCCACTTCATCGGCATCCGTGGCAACGGATGCCGAGTCTTCTCCGATGCGCAGCAAATCGCTGCCCATGAACTCTTCGAACaa                                                                                           <  8:224173/137‑1 (MQ=255)
                                                                 cggcggcgTGGCGTGCCGGACGCTGCGCGGCCAGGT‑TCCCCAGACGACGCTTCAGCCACTTCATCGGCATCCGTGGCAACGGATGCCGAGTCTTCTCCGATGCGCAGCAAATCGCTGCCCATGAACTCTTCGAACaa                                                                                           >  7:224173/1‑137 (MQ=255)
                                                                            cgtgCCGGACGCTGCGCGGCCAGGT‑TCCCCAGACGACGCTTCAGCCACTTCATCGGCATCCGTGGCAACGGATGCCGAGTCTTCTCCGATGCGCAGCAAATCGCTGCCCATGAACTCTTCGAACAAACCGCTGCGCGAGCCGAAAAggg                                                                    >  2:254059/1‑149 (MQ=255)
                                                                                 cGGACGCTGCGCGGCCAGGT‑TCCCCAGACGACGCTTCAGCCACTTCATCGGCATCCGTGGCACCGGATGCCGAGTCTTCTCCGATGCGCCGCAAATCGCTGCCCATGAACTCTGCGACCAAACCGCTGCGCGAGCCGAAAAGGGCTTcc                                                               >  7:231305/1‑149 (MQ=255)
                                                                                             ggCCAGGT‑TCCCCAGACGACGCTTCAGCCACTTCATCGGCATCCGTGGCAACGGATGCCGAGTCTTCTCCGATGCGCAGCAAATCGCTGCCCATGAACTCTTCGAACAAACCGCTGCGCGAGCCGAAAAGGGCTTCCAGTGGGGAAAgg                                                   <  7:187518/149‑1 (MQ=255)
                                                                                               ccAGGT‑TCCCCAGACGACGCTTCAGCCACTTCATCGGCATCCGTGGCAACGGATGCCTAGTCTTCTCCGATGCGCAGCAAATCGCTGCCCATGAACTCTTCGAACAAACCGCTGCGCGAGCCGAAAAGGGCTTCCAGTGGGGAAAGGGt                                                 <  4:249595/149‑1 (MQ=255)
                                                                                               ccAGGT‑TCCCCAGACGACGCTTCAGCCACTTCATCGGCATCCGTGGCAACGGATGCCGAGTCTTCTCCGATGCGCAGCAAATCGCTGCCCATGAACTCTTCGAACAAACCGCTGCGCGAGCCGAAAAGGGCTTCCAGTGGGGAAAGGGt                                                 >  4:3987/1‑149 (MQ=255)
                                                                                               ccAGGT‑TCCCCAGACGACGCTTCAGCCACTTCATCGGCATCCGTGGCAACGGATGCCGAGTCTTCTCCGATGCGCAGCAAATCGCTGCCCATGAACTCTTCGAACAAACCGCTGCGCGAGCCG‑AAAGGGCTTCCAGTGGGGAAAGGGTg                                                >  8:230601/1‑149 (MQ=255)
                                                                                                    t‑tCCCCAGACGACGCTTCAGCCACTTCATCGGCATCCGTGGCAACGGATGCCGAGTCTTCTCCGATGCGCAGCAAATCGCTGCCCATGAACTCTTCGAACAAACCGCTGCGCGAGCCGAAAAGGGCTTCCAGTGGGGAAAGGGTGCGtt                                            <  2:241973/149‑1 (MQ=255)
                                                                                                      ttaccAGACGACGCTTCAGCCACTTCATCGGCATCCGTGGCAACGGATGCCGAGTCTTCTCCGATGCGCAGCAAATCGCTGCCCATGAACTCTTCGAACAAACCGCTGCGCGAGCCGAAAAGGGCTTCCAGTGGGGAAAGGGTGCGtt                                            <  1:121307/145‑1 (MQ=255)
                                                                                                         ccAGACGACGCTTCAGCCACTTCATCGGCATCCGTGGCAACGGATGCCGAGTCTTCTCCGATGCGCAGCAAATCGCTGCCCATGAACTCTTCGAACAAACCGCTGCGCGAGCCGAAAAGGGCTTCCAGTGGGGAAAGGGTGCGTTTTTg                                        >  1:191333/1‑149 (MQ=255)
                                                                                                          cAGACGACGCTTCAGCCACTTCATCGGCATCCGTGGCAACGGATGCCGAGTCTTCTCCGATGCGCAGCAAATCGCTGCCCATGAACTCTTCGAACAAACCGCTGCGCGAGCCGAAAAGGGCTTCCAGTGGGGAAAGGGTGCGTTTTTGc                                       >  3:26983/1‑149 (MQ=255)
                                                                                                            gacgacGCTTCAGCCACTTCATCGGCATCCGTGGCAACGGATGCCGAGTCTTCTCCGATGCGCAGCAAATCGCTGCCCATGAACTCTTCGAACAAACCGCTGCGCGAGCCGAAAAGGGCTTCCAGTGGGGAAAGGGTGCGTTTTTGCTg                                     >  7:43755/1‑149 (MQ=255)
                                                                                                             acgacgCTTCAGCCACTTCATCGGCATCCGTGGCAACGGATGCCGAGTCTTCTCCGATGCGCAGCAAATCGCTGCCCATGAACTCTTCGAACAAACCGCTGCGCGAGCCGAAAAGGGCTTCCAGTGGGGAAAGGGTGCGTTTTTGCTgg                                    >  4:31224/1‑149 (MQ=255)
                                                                                                             acgacgCTTCAGCCACTTCATCGGCATCCGTGGCAACGGATGCCGAGTCTTCTCCGATGCGCAGCAAATCGCTGCCCATGAACTCTTCGAACAAACCGCTGCGCGAGCCGAAAAGGGCTTCCAGTGGGGAAAGGGTGCGTTTTTGCTgg                                    >  1:113060/1‑149 (MQ=255)
                                                                                                               gacgCTTCAGCCACTTCATCGGCATCCGTGGCAACGGATGCCGAGTCTTCTCCGATGCGCAGCAAATCGCTGCCCATGAACTCTTCGAACAAACCGCTGCGCGAGCCGAAAAGGGCTTCCAGTGGGGAAAGGGTGCGTTTTTGCTGgcg                                  >  4:24536/1‑149 (MQ=255)
                                                                                                                acgCTTCAGCCACTTCATCGGCATCCGTGGCAACGGATGCCGAGTCTTCTCCGATGCGCAGCAAATCGCTGCCCATGAACTCTTCGAACAAACCGCTGCGCGAGCCGAAAAgg                                                                     >  5:50338/1‑113 (MQ=255)
                                                                                                                  gCTTCAGCCACTTCATCGGCATCCGTGGCAACGGATGCCGAGTCTTCTCCGATGCGCAGCAAATCGCTGCCCATGAACTCTTCGAACAAACCGCTGCGCGAGCCGAAAAGGGCTTCCAGTGGGGAAAGGGTGCGTTTTTGCTGGCGCAc                               >  6:282137/1‑149 (MQ=255)
                                                                                                                    ttCAGCCACTTCATCGGCATCCGTGGCAACGGATGCCGAGTCTTCTCCGATGCGCAGCAAATCGCTGCCCATGAACTCTTCGAACAAACCGCTGCGCGAGCCGAAAAGGGCTTCCAGTGGGGAAAGGGTGCGTTTTTGCTGGCGCagc                              >  4:237839/1‑146 (MQ=255)
                                                                                                                      cAGCCACTTCATCGGCATCCGTGGCAACGGATGCCGAGTCTTCTCCGATGCGCAGCAAATCGCTGCCCATGAACTCTTCGAACAAACCGCTGCGCGAGCCGAAAAGGGCTTCCAGTGGGGAAAGGGTGCGTTTTTGCTGGCGCACCaa                            <  8:301389/148‑1 (MQ=255)
                                                                                                                         gagaagaaTCGGCATCCGTGGCAACGGATGCCGAGTCTTCTCCGATGCGCAGCAAATCGCTGCCCATGAACTCTTCGAACAAACCGCTGCGCGAGCCGAAAAGGGCTTCCAGTGGGGAAAGGGTGCGTTTTTGCTGGCGCACCAATTgg                        >  3:23841/8‑149 (MQ=255)
                                                                                                                         ccACTTCATCGGCATCCGTGGCAACGGATGCCGAGTCTTCTCCGATGCGCAGCAAATCGCTGCCCATGAACTCTTCGAACAAACCGCTGCGCGAGCCGAAAAGGGCTTCCAGTGGGGAAAGGGTGCGTTTTTGCTGGCGCACCAATTgg                        <  2:113060/149‑1 (MQ=255)
                                                                                                                           gaagaaTCGGCATCCGTGGCAACGGATGCCGAGTCTTCTCCGATGCGCAGCAAATCGCTGCCCATGAACTCTTCGAACAAACCGCTGCGCGAGCCGAAAAGGGCTTCCAGTGCGGAAAGGGTGCGTTTTTGCTGGCGCACCAATTGGCg                      <  4:23841/144‑1 (MQ=255)
                                                                                                                           aCTTCATCGGCATCCGTGGCAACGGATGCCGAGTCTTCTCCGATGCGCAGCAAATCGCTGCCCATGAACTCTTCGAACAAACCGCTGCGCGAGCCGAAAAGGGCTTCCAGTGGGGAAAGGGTGCGTTTTTGCTGGCGCACCAATTGGCg                      <  4:131936/149‑1 (MQ=255)
                                                                                                                           aCTTCATCGGCATCCGTGGCAACGGATGCCGAGTCTTCTCCGATGCGCAGCAAATCGCTGCCCATGAACTCTTCGAACAAACCGCTGCGCGAGCCGAAAAGGGCTTCCAGTGGGGAAAGGGTGCGTTTTTGCTGGCGCACCAATTGGCg                      <  3:24536/149‑1 (MQ=255)
                                                                                                                            cTTCATCGGCATCCGTGGCAACGGATGCCGAGTCTTCTCCGATGCGCAGCAAATCGCTGCCCATGAACTCTTCGAACAAACCGCTGCGCGAGCCGAAAAGGGCTTCCAGTGGGGAAAGGGTGCGTTTTTGCTGGCGCACCAATTGGCgg                     <  4:136324/149‑1 (MQ=255)
                                                                                                                               cATCGGCATCCTTTGCAACGGATGCCGAGTCTTCTCCGATGCGCAGCAAATCGCTGCCCATGAACTCTTCGAACAAACCGCTGCGCGAGCCGAAAAGGGCTTCCAGTGGGGAAAGGGTGCGTTTTTGCTGGCGCACCAATTGGCGGTa                   <  5:339052/148‑1 (MQ=255)
                                                                                                                               cATCGGCATCCTTGGCAACGGATGCCGAGTCTTCTCCGATGCGCAGCAAATCGCTGCCCATGAACTCTTCGAACAAACCGCTGCGCGAGCCGAAAAGGGCTTCCAGTGGGGAAAGGGTGCGTTTTTGCTGGCGCACCAATTGGCGGTa                   <  8:107266/148‑1 (MQ=255)
                                                                                                                                aTCGGCATCCGTGGCAACGGATGCCGAGTCTTCTCCGATGCGCAGCAAATCGCTGCCCATGAACTCTTCGAACAAACCGCTGCGCGAGCCGAAAAGGGCTTCCAGTGGGGAAAGGGTGCGTTTTTGCTGGCGCACCAATTGGCGGTAg                  <  5:197852/148‑1 (MQ=255)
                                                                                                                                aTCGGCATCCGTGGCAACGGATGCCGAGTCTTCTCCGATGCGCAGCAAATCGCTGCCCATGAACTCTTCGAACAAACCGCTGCGCGAGCCGAAAAGGGCTTCCAGTGGGGAAAGGGTGCGTTTTTGCTGGCGCACCAATTGGCGGTAg                  <  1:254059/148‑1 (MQ=255)
                                                                                                                                 tCGGCATCCGTTGCCACGGATGCCGAGTCTTCTCCGATGCGCAGCAAATCGCTGCCCATGAACTCTTCGAACAAACCGCTGCGCGAGCCGAAAAGGGCTTCCAGTGGGGAAAGGGTGCGttttt                                         <  6:51616/124‑1 (MQ=255)
                                                                                                                                 tCGGCATCCGTTGCCACGGATGCCGAGTCTTCTCCGATGCGCAGCAAATCGCTGCCCATGAACTCTTCGAACAAACCGCTGCGCGAGCCGAAAAGGGCTTCCAGTGGGGAAAGGGTGCGttttt                                         >  5:51616/1‑124 (MQ=255)
                                                                                                                                   ggCATCCGTGGCAACGGATGCCGAGTCTTCTCCGATGCGCAGCAAATCGCTGCCCATGAACTCTTCGAACAAACCGCTGCGCGAGCCGAAAAGGGCTTCCAGTGGGGAAAGGGTGCGTTTTTGCTGGCGCACCAATTGGCGGTAGTGat              <  2:65745/149‑1 (MQ=255)
                                                                                                                                    gCATCCGTTGCCACGGATGCCGAGTCTTCTCCGATGCGCAGCAAATCGCTGCCCATGAACTCTTCGAACAAACCGCTGCGCGAGCCGAAAAGGGCTTCCAGTGGGGAAAGGGTGCGTTTTTGCTGGCGCACAAATTGGCGGTTGTGat              >  6:29512/1‑148 (MQ=255)
                                                                                                                                    gCATCCGTGGCAACGGATGCCGAGTCTTCTCCGATGCGCAGCAAATCGCTGCCCATGAACTCTTCGAACAAACCGCTGCGCGAGCCGAAAAGGGCTTCCAGTGGGGAAAGGGTGCGTTTTTGCTGGCGCACCAATTGGCGGTAGTGatc             <  7:162900/149‑1 (MQ=255)
                                                                                                                                      aTCCGTGGCAACGGATGCCGAGTCTTCTCCGATGCGCAGCAAATCGCTGCCCATGAACTCTTCGAACAAACCGCTGCGCGAGCCGAAAAGGGCTTCCAGTGGGGAAAGGGTGCGTTTTTGCTGGCGCACCAATTGGCGGTAGTGatcat           <  2:191333/149‑1 (MQ=255)
                                                                                                                                           tGGCAACGGATGCCGAGTCTTCTCCGATGCGCAGCAAATCGCTGCCCATGAACTCTTCGAACAAACCGCTGCGCGAGCCGAAAAGGGCTTCCAGTGGGGAAAGGGTGCGTTTTTGCTGGCGCACCAATTGGCGGTAGTGATCATcacac      <  4:26983/149‑1 (MQ=255)
                                                                                                                                           tGGCAACGGATGCCGAGTCTTCTCCGATGCGCAGCAAATCGCTGCCCATGAACTCTTCGAACAAACCGCTGCGCGAGCCGAAAAGGGCTTCCAGTGGGGAAAGGGTGCGTTTTTGCTGGCGCACCAATTGGCGGTAGTGATCATcaca       >  2:105967/1‑148 (MQ=255)
                                                                                                                                              cAACGGATGCCGAGTCTTCTCCGATGCGCAGCAAATCGCTGCCCATGAACTCTTCGAACAAACCGCTGCGCGAGCCGAAAAGGGCTTCCAGT‑GGGAAAGGGTGCGTTTTTGCTGGCGCACCAATTGGCGGTAGTGATCATCACacaaca  <  3:121279/149‑1 (MQ=255)
                                                                                                                                               |                                                                                                                                                    
GCTTGGCGGCTTCCTGCAACAGGGCCTCGGAGTGTTCGCTGATGCGGCTGGCCAGCCCGCTGCTGCGGCGGCGTGGCGTGCCGGACGCTGCGCGGCCAGGT‑TCCCCAGACGACGCTTCAGCCACTTCATCGGCATCCGTGGCAACGGATGCCGAGTCTTCTCCGATGCGCAGCAAATCGCTGCCCATGAACTCTTCGAACAAACCGCTGCGCGAGCCGAAAAGGGCTTCCAGTGGGGAAAGGGTGCGTTTTTGCTGGCGCACCAATTGGCGGTAGTGATCATCACACAACA  >  NC_002947/3753456‑3753746

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑