Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,853,771 T→C 6.8% intergenic (+54/+28) PP_3401 → / ← PP_3402 hypothetical protein/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,853,7710TC 6.8% 213.2 / 4.3 73intergenic (+54/+28)PP_3401/PP_3402hypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (33/35);  new base C (2/3);  total (35/38)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.11e-01

CTTCGTCTGGGATGGGCGGAGTTATCACGGCGTGGTTCGCGAGAAGGGAAAGTGCGGCGACGGTGGCCTGAACCTGCTGCTGGAACTGC‑AATAAGCCGATCAGCGGCGGCCGTGGCCACGCTCGTGGCTGCTTATACCCGGCCAGCCTGGGCTGACCGGGGCACGACAGGCGCGCTCAGGCAATCGCCTTGAGTGGCGCCGGGTGGCTATCACGCAACGCATGCAGCTGCTCCACCAGGTTGACCAGGTCACGACAACTGTTGAGGTCTTGCAGCGCGACCCCGTTGAGGGTCAC  >  NC_002947/3853624‑3853918
                                                                                                                                                    |                                                                                                                                                   
cTTCGTCTGGGATTGGCGGAGTTATCACGGCGTGGTTCGCTAGAAGGGAAAGTGCGGCGACGGTGGCCTGAACCTGCTGCTGGAACTGC‑AATTAGGCGATCAGCGGCGGCCTTGGCCACGCTAGTGGCTGCTTATACCCGGACAGCCTg                                                                                                                                                    <  5:30387/149‑1 (MQ=255)
 ttCGTCTGGGATGGGCGGAGTTATCACGGCGTGGTTCGCGAGAAGGGAAAGTGCGGCGACGGTGGCCTGAACCTGCTGCTGGAACTGC‑AATAAGCGGATCAGCGGCGGCCGTGGCCACGCTCGTGGCTGCTTATACCCGGCCAGCCTgg                                                                                                                                                   >  7:248769/1‑149 (MQ=255)
   cGTCTGGGATGGGCGGAGTTATCACGGCGTGGTTCGCGAGAAGGGAAAGTGCGGCGACGGTGGCCTGAACCTGCTGCTGGAACTGC‑AATAAGCCGATCAGCGGCGGCCGTGGCCACGCTCGTGGCTGCTTATACCCGGCCAGCCTgg                                                                                                                                                   <  3:301959/147‑1 (MQ=255)
     tCTGGGATGGGCGGAGTTATCACGGCGTGGTTCGCGAGAAGGGAAAGTGCGGCGACGGTGGCCTGAACCTGCTGCTGGAACTGC‑AATAAGCCGATCAGCGGCGGCCGTGGCCACGCTCGTGGCTGCTTATACCCGGCCAGCCTGGGCTg                                                                                                                                               >  6:250497/1‑149 (MQ=255)
          gATGGGCGGAGTTATCACGGCGTGGTTCGCGAGAAGGGAAAGTGCGGCGACGGTGGCCTGAACCTGCTGCTGGAACTGC‑AATAAGCCGATCAGCGGCGGCCGTGGCCACGCTCGTGGCTGCTTATACCCGGTCAGCCCAGGCTGGCCgg                                                                                                                                          <  5:85857/149‑1 (MQ=255)
          gATGGGCGGAGTTATCACGGCGTGGTTCGCGAGAAGGGAAAGTGCGGCGACGGTGGCCTGAACCTGCTGCTGGAACTGC‑AATAAGCCGATCAGCGGCGGCCGTGGCCACGCTCGTGGCTGCTTATACCCGGCCAGCCTGGGCTGACCgg                                                                                                                                          <  3:37636/149‑1 (MQ=255)
             gggCGGAGTTATCACGGCGTGGTTCGCGAGAAGGGAAAGTGCGGCGACGGTGGCCTGAACCTGCTGCTGGAACTGC‑AATAAGCCGATCAGCGGCGGCCGTGGCCACGCTCGTGGCTGCTTATACCCGGCCAGCCTGGGCTGACCgggg                                                                                                                                        <  8:118357/148‑1 (MQ=255)
                 ggAGTTATCACGGCGTGGTTCGCGAGAAGGGAAAGTGCGGCGACGGTGGCCTGAACCTGCTGCTGGAACTGC‑AATAAGCCGATCAGCGGCGGCCGTGGCCACGCTCGTGGCTGCTTATACCCGGCCAGCCTGGGCTGACCGGGGCACGa                                                                                                                                   <  2:120855/149‑1 (MQ=255)
                    gTTATCACGGCGTGGTTCGCGAGAAGGGAAAGTGCGGCGACGGTGGCCTGAACCTGCTGCTGGAACTGC‑AATAAGCCGATCAGCGGCGGCCGTGGCCACGCTCGTGGCTGCTTATACCCGGCCAGCCTGGGCTGACCGGGGCACGACAg                                                                                                                                <  5:75215/149‑1 (MQ=255)
                       aTCACGGCGTGGTTCGCGAGAAGGGAAAGTGCGGCGACGGTGGCCTGAACCTGCTGCTGGAACTGC‑AATAAGCCGATCAGCGGCGGCCGTGGCCACGCTCGTGGCTGCTTATACCCGGCCAGCCTGGGCTGCCCGGGGCACGACAGgcg                                                                                                                             >  7:153161/1‑149 (MQ=255)
                       aTCACGGCGTGGTTCGCGAGAAGGGAAAGTGCGGCGACGGTGGCCTGAACCTGCTGCTGGAACTGC‑AATAAGCCGATCAGCGGCGGCCGTGGCCACGCTCGTGGCTGCTTATACCCGGCCAGCCTGGGCTGACCGGGGCACGACAGgc                                                                                                                              <  1:179235/148‑1 (MQ=255)
                       aTCACGGCGTGGTTCGCGAGAAGGGAAAGTGCGGCGACGGTGGCCTGAACCTGCTGCTGGAACTGC‑AATAAGCCGATCAGCGGCGGCCGTGGCCACGCTCGTGGCTGCTTATACCCGGCCAGCCTGGGCTGACCGGGGCACGACAGgc                                                                                                                              <  3:88002/148‑1 (MQ=255)
                       aTCACGGCGTGGTTCGCGAGAAGGGAAAGTGCGGCGACGGTGGCCTGAACCTGCTGCTGGAACTGC‑AATAAGCCGATCAGCGGCGGCCGTGGCCACGCTCGTGGCTGCTTATACCCGGCCAGCCTGGGCTGACCGGGGCACGACAGgc                                                                                                                              <  8:248769/148‑1 (MQ=255)
                        tCACGGCGTGGTTCGCGAGAAGGGAAAGTGCGGCGACGGTGGCCTGAACCTGCTGCTGGAACTGC‑AATAAGCCGATCAGCGGCGGCCGTGGCCACGCTCGTGGCTGCTTATACCCGGCCAGCCTGGGCTGACCGGGGCACGACAGgcgc                                                                                                                            >  2:113844/1‑149 (MQ=255)
                        tCACGGCGTGGTTCGCGAGAAGGGAAAGTGCGGCGACGGTGGCCTGAACCTGCTGCTGGAACTGC‑AATAAGCCGATCAGCGGCGGCCGTGGCCACGCTCGTGGCTGCTTATACCCGGCCAGCCTGGGCTGACCGGGGCACGACAGgcg                                                                                                                             <  5:250497/148‑1 (MQ=255)
                            ggCGTGGTTCGCGAGAAGGGAAAGTGCGGCGACGGTGGCCTGAACCTGCTGCTGGAACTGC‑AATAAGCCGATCAGCGGCGGCCGTGGCCACGCTCGTGGCTGCTTATACCCGGCCAGCCTGGGCTGACCGGGGCACGACAGGCGCGCt                                                                                                                         >  3:318889/1‑148 (MQ=255)
                             gCGTGGTTCGCGAGAAGGGAAAGTGCGGCGACGGTGGCCTGAACCTGCTGCTGGAACTGC‑AATAAGCCGATCAGCGGCGGCCGTGGCCACGCTCGTGGCTGCTTATACCCGGCCAGCCTGGGCTGACCGGGGCACGACAGGCGCGCTCa                                                                                                                       <  3:42209/149‑1 (MQ=255)
                             gCGGGGTTCGCGAGAAGGGAAAGTGCGGCGACGGTGGCCTGAACCTGCTGCTGGAACTGC‑AATAAGCCGATCAGCGGCGGCCGTGGCCACGCTCGTGGCTGCTTATACCCGGCCAGCCTGGGCTGACCGGGGCACGACAGGCGCGCTCa                                                                                                                       <  5:200397/149‑1 (MQ=255)
                                    tCGCGAGAAGGGAAAGTGCGGCGACGGTGGCCTGAACCTGCTGCTGGAACTGC‑AATAAGCCGATCAGCGGCGGCCGTGGCCACGCTCGTGGCTGCTTATACCCGGCCAGCCTGGGCTGACCGGGGCACGACAGGCGCGCTCAGGCAATc                                                                                                                <  1:234305/149‑1 (MQ=255)
                                        gagaAGGGAAAGTGCGGCGACGGTGGCCTGAACCTGCTGCTGGAACTGC‑AATAAGCCGATCAGCGGCGGCCGTGGCCACGCTCGTGGCTGCTTATACCCGGCCAGCCTGGGCTGACCGGGGCACGACAGGCGCGCTCAGGCAATCGCCt                                                                                                            >  1:305548/1‑149 (MQ=255)
                                        gagaAGGGAAAGTGCGGCGACGGTGGCCTGAACCTGCTGCTGGAACTGC‑AATAAGCCGATCAGCGGCGGCCGTGGCCACGCTCGTGGCTGCTTATACCCGGCCAGCCTGGGCTGACCGGGGCACGACAGGCGCGCTCAGGCAATCGCCt                                                                                                            >  6:183932/1‑149 (MQ=255)
                                             gggAAAGTGCGGCGACGGTGGCCTGAACCTGCTGCTGGAACTGC‑AATAAGCCGATCAGCGGCGGCCGTGGCCACGCTCGTGGCTGCTTATACCCGGCCAGCCTGGGCTGACCGGGGCACGACAGGCGCGCTCAGGCAATCGCCTTGAGt                                                                                                       <  6:25353/149‑1 (MQ=255)
                                                aaaGTGCGGCGACGGTGGCCTGAACCTGCTGCTGGAACTGC‑AATAAGACGATCAGCGGCGGCAGTGGCCACGCTCGTGGCTGCTTATACCCGGCCAGCCTGGGCTGACCGGGGCACGACAGGCGCGCTCAGGCAATCGCCTTGAGTGgc                                                                                                    >  4:118516/1‑149 (MQ=255)
                                                    tGCGGCGACGGTGGCCTGAACCTGCTGCTGGAACTGCGAATAAGCCGATCAGCGGCGGCCGTGGCCACGCTCGTGGCTGCTTATACCCGGCCAGCCTGGGCTGACCGGGGCACGACAGGCGCGCTCAGGCAATCGCCTTGAGTGGCGcc                                                                                                 >  1:231594/1‑149 (MQ=255)
                                                     gcggcgACGGTGGCCTGAACCTGCTGCTGGAACTGC‑AATAAGCCGATCAGCGGCGGCCGTGGCCACGCTCGTGGCTGCTTATACCCGGCCAGCCTGGGCTGACCGGGGCACGACAGGCGCGCTCAGGCAATCGCCTTGAGTGGCGCCgg                                                                                               >  7:101598/1‑149 (MQ=255)
                                                      cggcgACGGTGGCCTGAACCTGCTGCTGGAACTGC‑AATAAGCCGATCAGCGGCGGCCGTGGCCACGCTCGTGGCTGCTTATACCCGGCCAGCCTGGGCTGACCGGGGCACGACAGGCGCGCTCAGGCAATCGCCTTGAGTGGCGCCggg                                                                                              <  2:231594/149‑1 (MQ=255)
                                                       ggcgACGGTGGCCTGAACCTGCTGCTGGAACTGC‑AATAAGCCGATCAGCGGCGGCCGTGGCCACGCTCGTGGCTGCTTATACCCGGCCAGCCTGGGCTGACCGGGGCACGACAGGCGCGCTCAGGCAATCGCCTTGAGTGGCGCCGGGt                                                                                             <  1:86181/149‑1 (MQ=255)
                                                             ggTGGCCTGAACCTGCTGCTGGAACTGC‑AATAAGCCGATCAGCGGCGGCCGTGGCCACGCTCGTGGCTGCTTATACCCGGCCAGCCTGGGCTGACCGGGGCACGACAGGCGCGCTCAGGCAATCGCCTTGAg                                                                                                        >  7:264025/1‑132 (MQ=255)
                                                             ggTGGCCTGAACCTGCTGCTGGAACTGC‑AATAAGCCGATCAGCGGCGGCCGTGGCCACGCTCGTGGCTGCTTATACCCGGCCAGCCTGGGCTGACCGGGGCACGACAGGCGCGCTCAGGCAATCGCCTTGAg                                                                                                        <  8:264025/132‑1 (MQ=255)
                                                                   cTGAACCTGCTGCTGGAACTGC‑AATAAGCCGATCAGCGGCGGCCGTGGCCACGCTCGTGGCTGCTTATACCCGGCCAGCCTCGGCTGACCGGGGCACGACAGGCGCGCTCAGGCAATCGCCTTGAGTGGCGCCGGGTGGCTATCacgc                                                                                  >  2:236055/1‑148 (MQ=255)
                                                                         ctgctgctgGAACTGC‑AATAAGCCGATCAGCGGCGGCCGTGGCCACGCTCGTGGCTGCTTATACCCGGCCAGCCTGGGCTGACCGGGGCACGACAGGCGCGCTCAGGCAATCGCCTTGAGTGGCGCCGGTTGGCTATAACGCAACGAAt                                                                           >  6:235065/1‑149 (MQ=255)
                                                                         ctgctgctgGAACTGC‑AATAAGCCGATCAGCGGCGGCCGTGGCCACGCTCGTGGCTGCTTATACCCGGCCAGCCTGGGCTGACCGGGGCACGACAGGCGCGCTCAGGCAATCGCCTTGAGTGGCGCCGGGTGGCTATCACGCAACGCAt                                                                           >  6:223272/1‑149 (MQ=255)
                                                                         ctgctgctgGAACTGC‑AATAAGCCGATCAGCGGCGGCCGTGGCCACGCTCGTGGCTGCTTATACCCGGCCAGCCTGGGCTGACCGGGGCACGACAGGCGCGCTCAGGCAATCGCCTTGAGTGGCGCCGGGTGGCTATCACGCAACGCAt                                                                           <  7:85219/149‑1 (MQ=255)
                                                                            ctgctgGAACTGC‑AATAAGCCGATCAGCGGCGGCCGTGGCCACGCTCGTGGCTGCTTATACCCGGCCAGCCTGGGCTGACCGGGGCACGACAGGCGCGCTCAGGCAATCGCCTTGAGTGGCGCCGGGTg                                                                                            >  8:66517/1‑129 (MQ=255)
                                                                                tgGAACTGC‑AATAAGCCGATCAGCGGCGGCCGTGGCCACGCTCGTGGCTGCTTATACCCGGCCAGCCTGGGCTGACCGGGGCACGACAGGCGCGCTCAGGCAATCGCCTTGAGTGGCGCCGGGTGGCTATCACGCAACGCATGCAgctg                                                                    >  1:182319/1‑149 (MQ=255)
                                                                                    aCTGC‑AATAAGCCGATCAGCGGCGGCCGTGGCCACGCTCGTGGCTGCTTATACCCGGCCAGCCTGGGCTGACCGGGGCACGACAGGCGCGCTCAGGCAATCGCCTTGAGTGGCGCCGGGTGGCTATCacgcaac                                                                               >  2:284475/1‑134 (MQ=255)
                                                                                    aCTGC‑AATAAGCCGATCAGCGGCGGCCGTGGCCACGCTCGTGGCTGCTTATACCCGGCCAGCCTGGGCTGACCGGGGCACGACAGGCGCGCTCAGGCAATCGCCTTGAGTGGCGCCGGGTGGCTATCacgcaac                                                                               <  1:284475/134‑1 (MQ=255)
                                                                                    aCTGC‑AATAAGCCGATCAGCGGCGGCCGTGGCCACGCTCGTGGCTGCTTATACCCGGCCAGCCTGGGCTGACCGGGGCACGACAGGCGCGCTCAGGCAATCGCCTTGAGTGGCGCCGGGTGGCTATCACGCAACGCATGCAGCTGCTc                                                                 <  2:305548/148‑1 (MQ=255)
                                                                                      tGC‑AATAAGCCGATCAGCGGCGGCCGTGGCCACGCTCGTGGCTGCTTATTCCCGGCCAGCCTGGGCTGACCGGGGCACGACAGGCGCGCTCAGGCAATCGCCTTGAGTGGCGCCGGGTGGCTATCACGCAACGCATGCAGCTGCTccac                                                              >  1:260592/1‑149 (MQ=255)
                                                                                          aaTAAGCCGATCAGCGGCGGCCGTGGCCACGCTCGTGGCTGCTTATACCCGGCCAGCCTGGGCTGACCGGGGCACGACAGGCGCGCTCAGGCAATCGCCTTGAGTGGCGCCGGGTGGCt                                                                                         <  7:235284/119‑1 (MQ=255)
                                                                                          aaTAAGCCGATCAGCGGCGGCCGTGGCCACGCTCGTGGCTGCTTATACCCGGCCAGCCTGGGCTGACCGGGGCACGACAGGCGCGCTCAGGCAATCGCCTTGAGTGGCGCCGGGTGGCt                                                                                         >  8:235284/1‑119 (MQ=255)
                                                                                            tAAGCCGATCAGCGGCGGCCGTGGCCACGCTCGTGGCTGCTTATACCCGGCCAGCCTGGGCTGACCGGGGCACGACAGGCGCGCTCAGGCAATCGCCTTGAGTGGCGCCGGGTGGCTATCACGCAACGCATGCAGCTGCTCCACCAGGt                                                         >  5:280135/1‑149 (MQ=255)
                                                                                              aGCCGATCAGCGGCGGCCGTGGCCACGCTCGTGGCTGCTTATACCCGGCCAGCCTGGGCTGACCGGGGCACGACAGGCGCGCTCAGGCAATCGCCTTGAGTGGCGCCGGGTGGCTATCACGCAACGCATGCa                                                                        <  3:136789/132‑1 (MQ=255)
                                                                                              aGCCGATCAGCGGCGGCCGTGGCCACGCTCGTGGCTGCTTATACCCGGCCAGCCTGGGCTGACCGGGGCACGACAGGCGCGCTCAGGCAATCGCCTTGAGTGGCGCCGGGTGGCTATCACGCAACGCATGCa                                                                        >  4:136789/1‑132 (MQ=255)
                                                                                              aGCCGATCAGCGGCGGCCGTGGCCACGCTCGTGGCTGCTTATACCCGGCCAGCCTGGGCTGACCGGGGCACGACAGGCGCGCTCAGGCAATCGCCTTGAGTGGCGCCGGGTGGCTATCACGCAACGCATGCAGCTGCTCCACCAGGTTg                                                       <  8:160216/149‑1 (MQ=255)
                                                                                                ccGATCAGCGGCGGCCGTGGCCACGCTCGTGGCTGCTTATACCCGGCCAGCCTGGGCTGACCGGGGCACGACAGGCGCGCTCAGGCAATCGCCTTGAGTGGCGCCGGGTGGCTATCACGCAACGCATGCAGCTGCTCCACCAGGTTGAc                                                     <  6:280135/149‑1 (MQ=255)
                                                                                                  gATCAGCGGCGGCCGTGGCCACGCTCGTGGCTGCTTATACCCGGCCAGCCTGGGCTGACCGGGGCACGACAGGCGCGCTCAGGCAATCGCCTTGAGTGGCGCCGGGTGGCTATCACGCAACGCATGCAGCTGCTCCACCAGGTTGAc                                                     >  5:8578/1‑147 (MQ=255)
                                                                                                       gcggcgGCCGTGGCCACGCTCGTGGCTGCTTATACCCGGCCAGCCTGGGCTGACCGGGGCACGACAGGCGCGCTCAGGCAATCGCCTTGAGTGGCGCCGGGTGGCTATCACGCAACGCATGCAGCTGCTCCACCAGGTTGACCAGGTCa                                              <  8:101598/149‑1 (MQ=255)
                                                                                                       gcggcgGCCGTGGCCACGCTCGTGGCTGCTTATACCCGGCCAGCCTGGGCTGACCGGGGCACGACAGGCGCGCTCAGGCAATCGCCTTGAGTGGCGCCGGGTGGCTATCACGCAACGCATGCAGCTGCTCCACCAGGTTGACCAGGTCa                                              >  3:199144/1‑149 (MQ=255)
                                                                                                        cggcggCCGTGGCCACGCTCGTGGCTGCTTATACCCGGCCAGCCTGGGCTGACCGGGGCACGACAGGCGCGCTCAGGCAATCGCCTTGAGTGGCGCCGGGTGGCTATCACGCAACGCATGCAGCTGCTCCACCAGGTTGACCAGGTCAc                                             >  4:142442/1‑149 (MQ=255)
                                                                                                           cggcCGTGGCCACGCTCGTGGCTGCTTATACCCGGCCAGCCTGGGCTGACCGGGGCACGACAGGCGCGCTCAGGCAATCGCCTTGAGTGGCGCCGGGTGGCTATCACGCAACGCATGCAGCTGCTCCACCAGGTTGACCAGGTCACGAc                                          <  4:318889/149‑1 (MQ=255)
                                                                                                               cGTGGCCACGCTCGTGGCTGCTTATACCCGGCCAGCCTGGGCTGACCGGGGCACGACAGGCGCGCTCAGGCAATCGCCTTGAGTGGCGCCGGGTGGCTATCACGCAACGCATGCAGCTGCTCCACCAGGTTGACCAGGTCACGACAACt                                      <  8:25652/149‑1 (MQ=255)
                                                                                                                 tGGCCACGCTCGTGGCTGCTTATACCCGGCCAGCCTGGGCTGACCGGGGCACGACAGGCGCGCTCAGGCAATCGCCTTGAGTGGCGCCGGGTGGCTATCACGCAACACATGCAGCTGCTCCACCAGGTTGACCAGGTCACGACAACTGt                                    <  1:236055/149‑1 (MQ=255)
                                                                                                                     cACGCTCGTGGCTGCTTATACCCGGCCAGCCTGGGCTGACCGGGGCACGACAGGCGCGCTCAGGCAATCGCCTTGAGTGGCGCCGGGTGGCTATCACGCAACGCATGCAGCTGCTCCACCAGGTTGACCAGGTCACGACAACTGTTGAg                                <  5:223272/149‑1 (MQ=255)
                                                                                                                     cACGCTCGTGGCTGCTTATACCCGGCCAGCCTGGGCTGACCGGGGCACGACAGGCGCGCTCAGGCAATCGCCTTGAGTGGCGCCGGGTGGCTATCACGCAACGCATGCAGCTGCTCCACCAGGTTGACCAGGTCACGACAACTGTTGAg                                <  3:118516/149‑1 (MQ=255)
                                                                                                                          tCGTGGCTGCTTATACCCGGCCAGCCTGGGCTGACCGGGGCACGACAGGCGCGCTCAGGCAATCGCCTTGAGTGGCGCCGGGTGGCTATCACGCAACGCATGCAGCTGCTCCACCAGGTTGACCAGGTCACGACAACTGTTGAGGTCtt                           <  8:153161/149‑1 (MQ=255)
                                                                                                                            gTGGCTGCTTATACCCGGCCAGCCTGGGCTGACCGGGGCACGACAGGCGCGCTCAGGCAATCGCCTTGAGTGGCGCCGGGTGGCTATCACGCAACGCATGCAGCTGCTCCACCAGGTTGACCAGGTCACGACAACTGTTGAGGTCtt                           <  3:142442/147‑1 (MQ=255)
                                                                                                                              ggCTGCTTATACCCGGCCAGCCTGGGCTGACCGGGGCCCGACAGGCGCGCTCAGGCAATCGCCTTGAGTGGCGCCGGGTGGCTATCACGCAACGCATGCAGCTGCTCCACCAGGTTGACCAGGTCACGACAACTGTTGAGGTCTTGCAg                       >  5:227383/1‑149 (MQ=255)
                                                                                                                                  gctTATACCCGGCCAGCCTGGGCTGACCGGGGCACGACAGGCGCGCTCAGGCAATCGCCTTGAGTGGCGCCGGGTGGCTATCACGCAACGCATGCAGCTGCTCCACCAGGTTGACCAGGTCACGACAACTGTTGAGGTCTTGCAgcgc                    >  8:148179/1‑148 (MQ=255)
                                                                                                                                        aCCCGGCCAGCCTGGGCTGACCGGGGCACGACAGGCGCGCTCAGGCAATCGCCTTGAGTGGCGCCGGGTGGCTATCACGCAACGCATGCAGCTGCTCCACCAGGTTGACCAGGTCACGACAACTGTTGAGGTCTTGCAGCGCGACCCCg             >  5:234256/1‑149 (MQ=255)
                                                                                                                                        aCCCGGCCAGCCTGGGCTGACCGGGGCACGACAGGCGCGCTCAGGCAATCGCCTTGAGTGGCGCCGGGTGGCTATCACGCAACGCATGCAGCTGCTCCACCAGGTTGACCAGGTCACGACAACTGTTGAGGTCTTGCAGCGCGACCCCg             >  6:100296/1‑149 (MQ=255)
                                                                                                                                         ccggggcAGCCCAGGCTGGCGGGGGCACGACAGGCGCGCTCAGTCAATCGCCTTGAGTGGCGCCGGGTGGCTATCACGCAACGCATGCAGCTGCTCCACCAGGTTGACCAGGTCACGACAACTGTTGAGGTCTTGCAg                       <  5:204176/132‑1 (MQ=255)
                                                                                                                                         cccGGTCAGCCCAGGCTGGCCGGGGCACGACAGGCGCGCTCAGGCAATCGCCTTGAGTGGCGCCGGGTGGCTATCACGCAACGCATGCAGCTGCTCCAACAGGTTTACCAGGTCACGACAACTGTTGAGGACTTGCAg                       >  6:204176/1‑138 (MQ=255)
                                                                                                                                          ccGGTCAGCCCAGGCTGGCCGGGGCACGACAGGCGCGCTCAGGCAATCGCCTTGAGTGGCGCCGGGTGGCTATCACGCAACGCATGCAGCTGCTCCACCAGGTTGACCAGGTCACGACAACTGTTGAGGTCTTGCAGCGCGACCCCGtt           >  7:120204/1‑149 (MQ=255)
                                                                                                                                          ccGGCCAGCCTGGGCTGACCGGGGCACGACAGGCGCGCTCAGGCAATCGCCTTGAGTGGCGCCGGGTGGCTATCACGCAACGCATGCAGCTGCTCCACCAGGTTGACCAGGTCACGACAACTGTTGAGGTCTTGCAGCGCGACCCCGtt           >  7:3121/1‑149 (MQ=255)
                                                                                                                                          ccGGCCAGCCTGGGCTGACCGGGGCACGACAGGCGCGCTCAGGCAATCGCCTTGAGTGGCGCCGGGTGGCTATCACGCAACGCATGCAGCTGCTCCACCAGGTTGACCAGGTCACGACAACTGTTGAGGTCTTGCAGCGCGACCCCGtt           >  5:250117/1‑149 (MQ=255)
                                                                                                                                            ggCCAGCCTGGGCTGACCGGGGCACGACAGGCGCGCTCAGGCAATCGCCTTGAGTGGCGCCGGGTGGCTATCACGCAACGCATGCAGCTGCTCCACCAGGTTGACCAGGTCACGACAACTGTTGAGGTCTTGCAGCGCGACCCCGtt           >  8:53964/1‑147 (MQ=255)
                                                                                                                                             gCCAGCCTGGGCTGACCGGGGCACGACAGGCGCGCTCAGGCAATCGCCTTGAGTGGCGCCGGGTGGCTATCACGCAACGCATGCAGCTGCTCCACCAGGTTGACCAGGTCACGACAACTGTTGAGGTCTTGCAGCGCGACCCCGTTGAg        <  6:250117/149‑1 (MQ=255)
                                                                                                                                              tcAGCCCAGGCTGGCCGGGGCACGACAGGCGCGCTCAGGCAATCGCCTTGAGTGGCGCCGGGTGGCTATCACGCAACGCATGCAGCTGCTCCACCAGGTTGACCAGGTCACGACAACTGTTGAGGTCTTGCAGCGCGACCCCGTTGAgg       <  8:120204/148‑1 (MQ=255)
                                                                                                                                              ccAGCCTGGGCTGACCGGGGCACGACAGGCGCGCTCAGGCAATCGCCTTGAGTGGCGCCGGGTGGCTATCACGCAACGCATGCAGCTGCTCCACCAGGTTGACCAGGTCACGACAACTGTTGAGGTCTTGCAGCGCGACCCCGTTGAgg       <  5:183932/149‑1 (MQ=255)
                                                                                                                                               cAGCCTGGGCTGACCGGGGCACGACAGGCGCGCTCAGGCAATCGCCTTGAGTGGCGCCGGGTGGCTATCACGCAACGCATGCAGCTGCTCCACCAGGTTGACCAGGTCACGACAACTGTTGAGGTCTTGCAGCGCGACCCCGTTGAggg      <  8:3121/149‑1 (MQ=255)
                                                                                                                                                 gCCTGGGCTGACCGGGGCACGACAGGCGCGCTCAGGCAATCGCCTTGAGTGGCGCCGGGTGGCTATCACGCAACGCATGCAGCTGCTCCACCAGGTTGACCAGGTCACGACAACTGTTGAGGTCTTGCAGCGCGACCCCGTTGAGGGt     >  2:229766/1‑148 (MQ=255)
                                                                                                                                                   cTGGGCTGACCGGGGCACGACAGGCGCGCTCAGGCAATCGCCTTGAGTGGCGCCGGGTGGCTATCACGCAACGCATGCAGCTGCTCCACCAGGTTGACCAGGTCACGACAACTGTTGAGGTCTTGCAGCGCGACCCCGTTGAGGGTCAc  >  8:98310/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
CTTCGTCTGGGATGGGCGGAGTTATCACGGCGTGGTTCGCGAGAAGGGAAAGTGCGGCGACGGTGGCCTGAACCTGCTGCTGGAACTGC‑AATAAGCCGATCAGCGGCGGCCGTGGCCACGCTCGTGGCTGCTTATACCCGGCCAGCCTGGGCTGACCGGGGCACGACAGGCGCGCTCAGGCAATCGCCTTGAGTGGCGCCGGGTGGCTATCACGCAACGCATGCAGCTGCTCCACCAGGTTGACCAGGTCACGACAACTGTTGAGGTCTTGCAGCGCGACCCCGTTGAGGGTCAC  >  NC_002947/3853624‑3853918

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑