Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	3,894,392	G→T	8.8%	L223M (CTG→ATG) ‡	yegH ←	putative transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	3,894,392	0	G	T	8.8%	210.7 / 9.5	68	L223M (CTG→ATG) ‡	yegH	putative transporter
Reads supporting (aligned to +/- strand):  ref base G (29/33);  new base T (3/3);  total (32/36)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.94e-01

GCAATCTCCTCGCCGACCTCGTCAGCCTCGACCCGGCGGCCGCCCAGCAGGCGCAGCACGGCATGGGCAGTACGTTCGCGCAACGGCCTGTGCTGTTGCAGGCTGCGCTTGCGGCGGGCACGGGCCAGCTGGTTGAACAGCTCGATCAGGATCGAGAAGCCGATGGCCGCGTACAGGTAGCCTTTGGGGATATGGAAGCCCAGGCCTTCGGCGGTGAGGCTGAAACCGATCATCATCAGGAAGCCCAGGCACAGCATGATCACCGTGGGGTGGGCGTTGACGAAGCGGGTCAG  >  NC_002947/3894244‑3894536                                                                                                                                                     |                                                                                                                                                 gCAATCTCCTCGCCGACCTCGTCAGCCTCGACCCGGCGGCCGCCCAGCAGGCGCAGCACGGCATGGGCAGTACGTTCGCGCAACGGCCTGTGCTGTTGCAGGCTGCGCTTGCGGCGGGCACGGGCCAGCTGGTTGAACAGCTCGATCAg                                                                                                                                                  <  7:115779/149‑1 (MQ=255) gCAATCTCCTCGCCGACCTCGTCAGCCTCGACCCGGCGGCCGCCCAGCAGGCGCAGCACGGCATGGGCAGTACGTTCGCGCAACGGCCTGTGCTGTTGCAGGCTGCGCTTGCGGCGGGCACGGGCCAGCTGGTTGAACAGCTCGATCAg                                                                                                                                                  >  5:153899/1‑149 (MQ=255)  cAATCTCCTCGCCGACCTCGTCAGCCTCGACCCGGCGGCCGCCCAGCAGGCGCAGCACGGCATGGGCAGTACGTTCGCGCAACGGCCTGTGCTGTTGCAGGCTGCGCTTGCGGCGGGCACGGGCCAGCTGGTTGAACAGCTCGATCAg                                                                                                                                                  >  6:176773/1‑148 (MQ=255)        cctcGCCGACCTCGTCAGCCTCGACCCGGCGGCCGCCCAGCAGGCGCAGCACGGCATGGGCAGTACGTTCGCGCAACGGCCTGTGCTGTTGCAGGCTGCGCTTGCGGCGGGCACGGGCCAGCTGGTTGAACAGCTCGATCAGGATCg                                                                                                                                             <  2:238116/147‑1 (MQ=255)        cctcGCCGACCTCGTCAGCCTCGACCCGGCGGCCGCCCAGCAGGCCCAGCACGGCATGGGCAGTACGTTCGCGCAACGGCCTGTGCTGTTGCAGGCTGCGCTTGCGGCGGGCACGGGCCAGCTGGTTGAACAGCTCGATCAGGATCg                                                                                                                                             >  1:238116/1‑147 (MQ=255)         ctcGCCGACCTCGTCAGCCTCGACCCGGCGGCCGCCCAGCAGGCGCAGCACGGCATGGGCAGTACGTTCGCGCAACGGCTTGTGCTGTTGCAGGCTGCGCTTGCGGCGGGCACGGGCCAGCTGGTTGAACAGCTCGATCAGGATCgag                                                                                                                                           <  2:187015/148‑1 (MQ=255)         ctcGCCGACCTCGTCAGCCTCGACCCGGCGGCCGCCCAGCAGGCGCAGCACGGCATGGGCAGTACGTTCGCGCAACGGCCTGTGCTGTTGCAGGCTGCGCTTGCGGCGGGCACGGGCCAGCTGGTTGAACAGCTCGATCCTGATc                                                                                                                                              <  5:15071/145‑1 (MQ=255)         ctcGCCGACCTCGTCAGCCTCGACCCGGCGGCCGCCCAGCAGGCGCAGCACGGCATGGGCAGTACGTTCGCGCAACGGCCTGTGCTGTTGCAGGCTGCGCTTGCGGCGGGCACGGGCCAGCTGGTTGAACAGCTCGATCAGGATc                                                                                                                                              <  3:210652/145‑1 (MQ=255)            gCCGACCTCGTCAGCCTCGACCCGGCGGCCGCCCAGCAGGCGCAGCACGGCATGGGCAGTACGTTCGCGCAACGGCCTGTGCTGTTGCAGGCTGCGCTTGCGGCGGGCACGGGCCAGCTGGTTGAACAGCTCGATCCTGATCgagctgt                                                                                                                                       <  4:277314/149‑5 (MQ=255)            gCCGACCTCGTCAGCCTCGACCCGGCGGCCGCCCAGCAGGCGCAGCACGGCATGGGCAGTACGTTCGCGCAACGGCCTGTGCTGTTGCAGGCTGCGCTTGCGGCGGGCACGGGCCAGCTGGTTGAACAGCTCGATCAGGATCGAGaa                                                                                                                                         <  3:250661/147‑1 (MQ=255)             ccGACCTCGTCAGCCTCGACCCGGCGGCCGCCCAGCAGGCGCAGCACGGCATGGGCAGTACGTTCGCGCAACGGCCTGTGCTGTTGCAGGCTGCGCTTGCGGCGGGCACGGGCCAGCTGGTTGAACAGCTCGATCAGGATCGAGAAGcc                                                                                                                                      <  5:35515/149‑1 (MQ=255)                 ccTCGTCAGCCTCGACCCGGCGGCCGCCCAGCAGGCGCAGCACGGCATGGGCAGTACGTTCGCGCAACGGCCTGTGCTGTTGCAGGCTGCGCTTGCGGCGGGCACGGGCCAGCTGGTTGAACAGCTCGATCAGGATCGAGAAGCCGATg                                                                                                                                  <  3:328276/149‑1 (MQ=255)                 ccTCGTCAGCCTCGACCCGGCGGCCGCCCAGCAGGCGCAGCACGGCATGGGCAGTACGTTCGCGCAACGGCCTGTGCTGTTGCAGGCTGCGCTTGCGGCGGGCACGGGCCAGCTGGTTGAACAGCTCGATCAGGATCGAGAAGCCGATg                                                                                                                                  <  5:176773/149‑1 (MQ=255)                  cTCGTCAGCCTCGACCCGGCGGCCGCCCAGCAGGCGCAGCACGGCATGGGCAGTACGTTCGCGCAACGGCCTGTGCTGTTGCAGGCTGCGCTTGCGGCGGGCCCGGGCCAGCTGGTTGAACAGCTCGATCAGGATCGAGAAGCCGATgg                                                                                                                                 >  5:192986/1‑149 (MQ=255)                     gTCAGCCTCGACCCGGCGGCCGCCCAGCAGGCGCAGCACGGCATGGGCAGTACGTTCGCGCAACGGCCTGTGCTGTTGCAGGCTGCGCTTGCGGCGGGCACGGGCCAGCTGGTTGAACAGCTCGATCAGGATCGAGAAGCCGATGGcc                                                                                                                               <  8:194327/148‑1 (MQ=255)                          ccTCGACCCGGCGGCCGCCCAGCAGGCGCAGCACGGCATGGGCAGTACGTTCGCGCAACGGCCTGTGCTGTTGCAGGCTGCGCTTGCGGCGGGCACGGGCCAGCTGGTTGAACAGCTCGATCAGGATCGAGAAGCCGATGGCCGCGTAc                                                                                                                         <  6:153899/149‑1 (MQ=255)                           cTCGACCCGGCGGCCGCCCAGCAGGCGCAGCACGGCATGGGCAGTACGTTCGCGCAACGGCCTGTGCTGTTGCAGGCTGCGCTTGCGGCGGGCACGGGCCAGCTGGTTGAACAGCTCGATCAGGATCGAGAAGCCGATGGCCGCGTa                                                                                                                          >  6:211803/1‑147 (MQ=255)                              gACCCGGCGGCCGCCCAGCAGGCGCAGCACGGCATGGGCAGTACGTTCGCGCAACGGCCTGTGCTGTTGCAGGCTGCGCTTGCGGCGGGCACGGGCCAGCTGGTTGAACAGCTCGATCAGGATCGAGAAGCCGATGGCCGCGTACAg                                                                                                                       <  1:308668/147‑1 (MQ=255)                                 ccGGCGGCCGCCCAGCAGGCGCAGCACGGCATGGGCAGTACGTTCGCGCAACGGCCTGTGCTGTTGCAGGCTGCGCTTGCGGCGGGCACGGGCCAGCTGGTTGAACAGCTCGATCAGGATCGAGAAGCCGATGGCCGCGTACAGGTAGc                                                                                                                  >  7:85857/1‑149 (MQ=255)                                       gccgccCAGCAGGCGCAGCACGGCATGGGCAGTACGTTCGCGCAACGGCCTGTGCTGTTGCAGGCTGCGCTTGCGGCGGGCACGGGCCAGCTGGTTGAACAGCTCGATCAGGATCGAGAAGCCGATGGCCGCGTACAGGTAGCCTTTgg                                                                                                            <  7:110258/149‑1 (MQ=255)                                        ccgccCAGCAGGCGCAGCACGGCATGGGCAGTACGTTCGCGCAACGGCCTGTGCTGTTGCAGGCTGCGCTTGCGGCGGGCACGGGCCAGCTGGTTGAACAGCTCGATCAGGATCGAGAAGCCGATGGCCGCGTACAGGTAGCCTTTggg                                                                                                           <  2:63625/149‑1 (MQ=255)                                         cgccCAGCAGGCGCAGCACGGCATGGGCAGTACGTTCGCGCAACGGCCTGTGCTGTTGCAGGCTGCGCTTGCGGCGGGCACGGGCCAGCTGGTTGAACAGCTCGATCAGGATCGAGAAGCCGATGGCCGCGTACAGGTAGCCTTTggg                                                                                                           >  2:111476/1‑148 (MQ=255)                                         cgccCAGCAGGCGCAGCACGGCATGGGCAGTACGTTCGCGCAACGGCCTGTGCTGTTGCAGGCTGCGCTTGCGGCGGGCACGGGCCAGCTGGTTGAACAGCTCGATCAGGATCGAGAAGCCGATGGCCGCGTACAGGTAGCCTTTggg                                                                                                           >  4:296614/1‑148 (MQ=255)                                          gccCAGCAGGCGCAGCACGGCATGGGAAGTACGTTCGCGCAACGGCCTGTGCTGTTGCAGGCTGCGCTTGCGGCGGGCACCGGCAAGATGGTTGATCAGCTCGATAAGGATCGAGAAGCCGAGGGCCGCGTACAGGTAGCCTTTGGGGa                                                                                                         >  6:313889/1‑149 (MQ=255)                                                            ggCATGGGCAGTACGTTCGCGCAACGGCCTGTGCTGTTGCAGGCTGCGCTTGCGGCGGGCACGGGCCAGCTGGTTGAACAGCTCGATCAGGATCGAGAAGCCGATGGCCGCGTACAGGTAGCCTTTGGGGATATGGAAGCCCAGGCCtt                                                                                       >  4:254862/1‑149 (MQ=255)                                                                tGGGCAGTACGTTCGCGCAACGGCCCGTGCTGTTGCAGGCTGCGCTTGCGGCGGGCACGGGCCAGCTGGTTGAACAGCTCGATCAGGATCGAGAAGCCGATGGCCGCGTACAGGTAGCCTTTGGGGATATGGAAGCCCAGGCCTTcggc                                                                                   >  1:105607/1‑149 (MQ=255)                                                                  ggCAGTACGTTCGCGCAACGGCCTGTGCTGTTGCAGGCTGCGCTTGCGGCGGGCACGGGCCAGCTGGTTGAACAGCTCGATCAGGATCGAGAAGCCGATGGCCGCGTACAGGTAGCCTTTGGGGATATGGAAGCCCAGGCCTTcggcgg                                                                                 <  3:296614/149‑1 (MQ=255)                                                                  ggCAGTACGTTCGCGCAACGGCCTGTGCTGTTGCAGGCTGCGCTTGCGGCGGGCACGGGCCAGCTGGTTGAACAGCTCGATCAGGATCGAGAAGCCGATGGCCGCGTACAGGTAGCCTTTGGGGATATGGAAGCCCAGGCCTTcggcgg                                                                                 <  3:324469/149‑1 (MQ=255)                                                                         cGTTCGCGCAACGGCCCGTGCTGTTGCAGGCTGCGCTTGCGGCGGGCACGGGCCAGCTGGTTGAACAGCTCGATCAGGATCGAGAAGCCGATGGCCGCGTACAGGTAGCCTTTGGGGATATGGAAGCCCAGGCCTTCGGCGGTGAGGCt                                                                          <  2:105607/149‑1 (MQ=255)                                                                          gTTCGCGCAACGGCCTGTGCTGTTGCAGGCTGCGCTTGCGGCGGGCACGGGCCAGCTGGTTGAACAGCTCGATCAGGATCGAGAAGCCGATGGCCGCGTACAGGTAGCCTTTGGGGATATGGAAGCCCAGGCCTTCGGCGGTGAGGCt                                                                          >  4:69083/1‑148 (MQ=255)                                                                           ttCGCGCAACGGCCTGTGCTGTTGCAGGCTGCGCTTGCGGCGGGCACGGGCCAGCTGGTTGAACAGCTCGATCAGGATCGAGAAGCCGATGGCCGCGTACAGGTAGCCTTTGGGGATATGGAAGCCCAGGCCTTCGGCGGTGAGGCTGa                                                                        <  8:85857/149‑1 (MQ=255)                                                                              gcgcAACGGCCTGTGCTGTTGCAGGCTGCGCTTGCGGCGGGCACGGGCCAGCTGGTTGAACAGCTCGATCAGGATCGAGAAGCCGATGGCCGCGTACAGGTAGCCTTTGGGGATATGGAAGCCCAGGCCTTCGGCGGTGAGGCTGAAAc                                                                     >  4:300691/1‑149 (MQ=255)                                                                              gcgcAACGGCCTGTGCTGTTGCAGGCTGCGCTTGCGGCGGGCACGGGCCAGCTGGTTGAACAGCTCGATCAGGATCGAGAAGCCGATGGCCGCGTACAGGTAGCCTTTGGGGATATGGAAGCCCAGGCCTTCGGCGGTGAGGCTGAAAc                                                                     >  4:305522/1‑149 (MQ=255)                                                                                 cAACGGCCTGTGCTGTTGCAGGCTGCGCTTGCGGCGGGCACGGGCCAGCTGGTTGAACAGCTCGATCAGGATCGAGAAGCCGATGGCCGCGTACAGGTAGCCTTTGGGGATATGGAAGCCCAGGCCTTCGGCGGTGAGGCTGAAACCGa                                                                  <  3:22553/149‑1 (MQ=255)                                                                                  aaCGGCCTGTGCTGTTGCAGGCTGCGCTTGCGGCGGGCACGGGCCAGCTGGTTGAACAGCTCGATCAGGATCGAGAAGCCGATGGCCGCGTACAGGTAGCCTTTGGGGATATGGAAGCACAGGCCTTCGGCGGTGAGGCTGAAACCGa                                                                  >  6:8322/1‑148 (MQ=255)                                                                                    cGGCCTGTGCTGTTGCAGGCTGCGCTTGCGGCGGGCACGGGCCAGCTGGTTGAACAGCTCGATCAGGATCGAGAAGCCGATGGCCGCGTACAGGTAGCCTTTGGGGATATGGAAGCCCAGGCCTTCGGCGGTGAGGCTGAAACCGatca                                                               >  2:308195/1‑149 (MQ=255)                                                                                         tgtgCTGTTGCAGGCTGCGCTTGCGGCGGGCACGGGCCAGCTGGTTGAACAGCTCGATCAGGATCGAGAAGCCGATGGCCGCGTACAGGTAGCCTTTGGGGATATGGAAGCCCAGGCCTTCGGCGGTGAGGCTGaaa                                                                      >  1:339482/1‑137 (MQ=255)                                                                                         tgtgCTGTTGCAGGCTGCGCTTGCGGCGGGCACGGGCCAGCTGGTTGAACAGCTCGATCAGGATCGAGAAGCCGATGGCCGCGTACAGGTAGCCTTTGGGGATATGGAAGCCCAGGCCTTCGGCGGTGAGGCTGaaa                                                                      <  2:339482/137‑1 (MQ=255)                                                                                           tgCTGTTGCAGGCTGCGCTTGCGGCGGGCACGGGCCAGCTGGTTGAACAGCTCGATCAGGATCGAGAAGCCGATGGCCGCGTACAGGTAGCCTTTGGGGATATGGAAGCCCAGGCCTTCGGCGGTGAGGCTGAAACCGatcatcatc                                                          <  8:196798/147‑1 (MQ=255)                                                                                           tgCTGTTGCAGGCTGCGCTTGCGGCGGGCACGGGCCAGCTGGTTGAACAGCTCGATCAGGATCGAGAAGCCGATGGCCGCGTACAGGTAGCCTTTGGGGATATGGAAGCCCAGGCCTTCGGCGGTGAGGCTGAAACCGAtcatcatca                                                         <  5:211803/148‑1 (MQ=255)                                                                                                ttGCAGGCTGCGCTTGCGGCGGGCACGGGCCAGCTGGTTGAACAGCTCGATCAGGATCGAGAAGCCGATGGCCGCGTACAGGTAGCCTTTGGGGATATGGAAGCCCAGGCCTTCGGCGGTGAGGCTGAAACCGATCATCATCAGGaa                                                     <  1:308195/147‑1 (MQ=255)                                                                                                ttGCAGGCTGCGCTTGCGGCGGGCACGGGCCAGCTGGTTGAACAGCTCGATCAGGATCGAGAAGCCGATGGCCGCGTACAGGTAGCCTTTGGGGATATGGAAGCCCAGGCCTTCGGCGGTGAGGCTGAAACCGATCATCATCAGGAAGc                                                   >  1:256606/1‑149 (MQ=255)                                                                                                  gCAGGCTGCGCTTGCGGCGGGCATGGGCCAGCTGGTTGAACAGCTCGATCAGGATCGAGAAGCCGATGGCCGCGTACAGGTAGCCTTTGGGGATATGGAAGCCCAGGCCTTCGGCGGTGAGGCTGAAACCGATCATCATCAGGAAGcc                                                  >  2:322607/1‑148 (MQ=255)                                                                                                  gCAGGCTGCGCTTGCGGCGGGCACGGGCCAGCTGGTTGAACAGCTCGATCAGGATCGAGAAGCCGATGGCCGCGTACAGGTAGCCTTTGGGGATATGGAAGCCCAGGCCTTCGGCGGTGAGGCTGAAACCGATCATCATCAGGAAGcc                                                  >  7:260693/1‑148 (MQ=255)                                                                                                   cAGGCTGCGCTTGCGGCGGGCACGGGCCAGCTGGTTGAACAGCTCGATCAGGATCGAGAAGCCGATGGCCGCGTACAGGTAGCCTTTGGGGATATGGAAGCCCAGGCCTTCGGCGGTGAGGCTGAAACCGATCATCATCAGGAAGccc                                                 <  8:260693/148‑1 (MQ=255)                                                                                                     ggCTGCGCTTGCGGCGGGCACGGGCCAGCTGGTTGAACAGCTCGATCAGGATCGAGAAGCCGATGGCCGCGTACAGGTAGCCTTTGGGGATATGGAAGCCCAGGCCTTCGGCGGTGAGGCTGAAACCGATCATCATCAGGAAGCCCAgg                                              <  1:111476/149‑1 (MQ=255)                                                                                                               gcggcgGGCACGGGCCAGCTGGTTGAACAGCTCGATCAGGATCGAGAAGCCGATGGCCGCGTACAGGTAGCCTTTGGGGATATGGAAGCCCAGGCCTTCGGCGGTGAGGCTGAAACCGATCATCATCAGGAAGCCCAGGCACAGCATg                                     <  3:69083/148‑1 (MQ=255)                                                                                                                   cggGCACGGGCCAGCTGGTTGAACAGCTCGATCAGGATCGAGAAGCCGATGGCCGCGTACAGGTAGCCTTTGGGGATATGGAAGCCCAGGCCTTCGGCGGTGAGGCTGAAACCGATCATCATCAGGAAGCCCAGGCACAGCATGATCAc                                <  3:300691/149‑1 (MQ=255)                                                                                                                   cggGCACGGGCCAGCTGGTTGAACAGCTCGATCAGGATCGAGAAGCCGATGGCCGCGTACAGGTAGCCTTTGGGGATATGGAAGCCCAGGCCTTCGGCGGTGAGGCTGAAACCGATCATCATCAGGAAGCCCAGGCACAGCATGATCAc                                <  3:305522/149‑1 (MQ=255)                                                                                                                      gCACGGGCCAGCTGGTTGAACAGCTCGATCAGGATCGAGAAGCCGATGGCCGCGTACAGGTAGCCTTTGGGGATATGGAAGCCCAGGCCTTCGGCGGTGAGGCTGAAACCGATCATCATCAGGAAGCCCAgg                                              <  4:313178/132‑1 (MQ=255)                                                                                                                      gCACGGGCCAGCTGGTTGAACAGCTCGATCAGGATCGAGAAGCCGATGGCCGCGTACAGGTAGCCTTTGGGGATATGGAAGCCCAGGCCTTCGGCGGTGAGGCTGAAACCGATCATCATCAGGAAGCCCAgg                                              >  3:313178/1‑132 (MQ=255)                                                                                                                       catgggCCAGCTGGTTGAACAGCTCGATCAGGATCGAGAAGCCGATGGCCGCGTACAGGTAGCCTTTGGGGATATGGAAGCCCAGGCCTTCGGCGGTGAGGCTGAAACCGATCATCATCAGGAAGCCCAGGCACAGCATGATCACCgtg                            <  1:322607/146‑1 (MQ=255)                                                                                                                       cACGGGCCAGCTGGTTGAACAGCTCGATCAGGATCGAGAAGCCGATGGCCGCGTACAGGTAGCCTTTGGGGATATGGAAGCCCAGGCCTTCGGCGGTGAGGCTGAAACCGATCATCATCAGGAAGCCCAGGCACAGCATGATCACCgtg                            >  3:108249/1‑149 (MQ=255)                                                                                                                           ggCCAGCTGGTTGAACAGCTCGATCAGGATCGAGAAGCCGATGGCCGCGTACAGGTAGCCTTTGGGGATATGGAAGCCCAGGCCTTCGGCGGTGAGGCTGAAACCGATCATCATCAGGAAGCCCAGGCACAGCATGATCACCgtggggt                        <  7:154507/149‑1 (MQ=255)                                                                                                                              cAGCTGGTTGAACAGCTCGATCAGGATCGAGAAGCCGATGGCCGCGTACAGGTAGCCTTTGGGGATATGGAAGCCCAGGCCTTCGGCGGTGAGGCTGAAACCGATCATCATCAGGAAGCCCAGGCACAGCATGATCACCgtggggtggg                     >  3:318310/1‑149 (MQ=255)                                                                                                                               aGCTGGTTGAACAGCTCGATCAGGATCGAGAAGCCGATGGCCGCGTACAGGTAGCCTTTGGGGATATGGAAGCCCAGGCCTTCGGCGGTGAGGCTGAAACCGATCATCATCAGGATGCCCAGGCACAGCATGATCACCGTGGGGTGGGc                    >  8:111149/1‑149 (MQ=255)                                                                                                                                 cTGGTTGAACAGCTCGATCAGGATCGAGAAGCCGATGGCCGCGTACAGGTAGCCTTTGGGGATATGGAAGCCCAGGCCTTCGGCGGTGAGGCTGAAACCGATCATCATCAGGAAGCCCAGGCACAGCATGATCACCGTGGGGTGGGCGt                  <  6:192986/149‑1 (MQ=255)                                                                                                                                    gTTGAACAGCTCGATCAGGATCGAGAAGCCGATGGCCGCGTACAGGTAGCCTTTGGGGATATGGAAGCCCAGGCCTTCGGCGGTGAGGCTGACACCGATTATAATCAGGAAGCCCAGGCACAGCAGGCTCACCGTGTGGTGGGCGtggc               >  4:52582/1‑146 (MQ=255)                                                                                                                                    gTTGAACAGCTCGATCAGGATCGAGAAGCCGATGGCCGCGTACAGGTAGCCTTTGGGGATATGGAAGCCCAGGCCTTCGGCGGTGAGGCTGAAACCGATCATCATCAGGACGCCCAGGTACAGCATGATCACCGTGGGGTGGGCGTTGa               >  6:4805/1‑149 (MQ=255)                                                                                                                                    gTTGAACAGCTCGATCAGGATCGAGAAGCCGATGGCCGCGTACAGGTAGCCTTTGGGGATATGGAAGCCCAGGCCTTCGGCGGTGAGGCTGAAACCGATCATCATCAGGAAGCCCAGGCACAGCATGATCACCGTGGGGTGGGCGTTGa               >  8:220042/1‑149 (MQ=255)                                                                                                                                      tcggcttcTCGATCCTGATCGAGCAGCCGATGGCCGCGTACAGGTAGCCTTTGGGGATATGGAAGCCCAGGCCTTCGGCGGTGAGGCTGAAACCGATCATCATCAGGAAGCCCAGGCACAGCATGATCACCGTGGGGTGGGCGTTGAc              >  6:57596/8‑148 (MQ=255)                                                                                                                                        aaCAGCTCGATCAGGATCGAGAAGCCGATGGCCGCGTACAGGTAGCCTTTGGGGATATGGAAGCCCAGGCCTTCGGCGGGGAGGCTGACACCGATCATCCTCAGGAAGCCCAGGCAGAGCATGCTCACCCTGGGGTGGGCGTGGACGac           >  8:138198/1‑148 (MQ=255)                                                                                                                                          cttcTCGATCCTGATCGAGAAGCCGATGGCCGCGTACAGGTAGCCTTTGGGGATATGGAAGCCCAGGCCTTCGGCGGTGAGGCTGAAACCGATCATCATCAGGAAGCCCAGGCACAGCATGATCACCGTGGGGTGGGCGTTGACGAAGc         >  5:257183/4‑149 (MQ=255)                                                                                                                                           ttcTCGATCCTGATCGAGAAGCCGATGGCCGCGTACAGGTAGCCTTTGGGGATATGGAAGCCCAGGCCTTCGGCGGTGAGGCTGAAACCGATCATCATCAGGAAGCCCAGGCACAGCATGATCACCgtggggtg                       >  1:14796/3‑134 (MQ=255)                                                                                                                                           ttcTCGATCCTGATCGAGAAGCCGATGGCCGCGTACAGGTAGCCTTTGGGGATATGGAAGCCCAGGCCTTCGGCGGTGAGGCTGAAACCGATCATCATCAGGAAGCCCAGGCACAGCATGATCACCgtggggtg                       <  2:14796/132‑1 (MQ=255)                                                                                                                                            gCTCGATCAGGATCGAGAAGCCGATGGCCGCGTACAGGTAGCCTTTGGGGATATGGAAGCCCAGGCCTTCGGCGGTGAGGCTGAAACCGATCATCATCAGGAAGCCCAGGCACAGCATGATCACCGTGGGGTGGGCGTTGACGAAGCgg       <  4:318310/149‑1 (MQ=255)                                                                                                                                             cTCGATCAGGATCGAGAAGCCGATGGCCGCGTACAGGTAGCCTTTGGGGATATGGAAGCCCAGGCCTTCGGCGGTGAGGCTGAAACCGATCATCATCAGGAAGCCCAGGCACAGCATGATCACCGTGGGGTGGGCGTTGACGAAGCggg      >  1:315681/1‑149 (MQ=255)                                                                                                                                                gATCAGGATCGAGAAGCCGATGGCCGCGTACAGGTAGCCTTTGGGGATATGGAAGCCCAGGCCTTCGGCGGTGAGGCTGAAACCGATCATCATCAGGAAGCCCAGGCACAGCATGATCACCGTGGGGTGGGCGTTGACGAAGCGGGt     >  8:103769/1‑147 (MQ=255)                                                                                                                                                gATCAGGATCGAGAAGCCGATGGCCGCGTACAGGTAGCCTTTGGGGATATGGAAGCCCAGGCCTTCGGCGGTGAGGCTGAAACCGATCATCATCAGGAAGCCCA‑GCACAGCATGATCACCgtggg                          >  5:98534/1‑125 (MQ=255)                                                                                                                                                gATCAGGATCGAGAAGCCGATGGCCGCGTACAGGTAGCCTTTGGGGATATGGAAGCCCAGGCCTTCGGCGGTGAGGCTGAAACCGATCATCATCAGGAAGCCCA‑GCACAGCATGATCACCgtggg                          <  6:98534/125‑1 (MQ=255)                                                                                                                                                 aTCAGGATCGAGAAGCCGATGGCCGCGTACAGGTAGCCTTTGGGGATATGGAAGCCCAGGCCTTCGGCGGTGAGGCTGAAACCGATCATCATCAGGAAGCCCAGGCACAGCATGATCACCGTGGGGTGGGCGTTGACGAAGCGGGTCAg  <  7:220042/149‑1 (MQ=255)                                                                                                                                                     |                                                                                                                                                 GCAATCTCCTCGCCGACCTCGTCAGCCTCGACCCGGCGGCCGCCCAGCAGGCGCAGCACGGCATGGGCAGTACGTTCGCGCAACGGCCTGTGCTGTTGCAGGCTGCGCTTGCGGCGGGCACGGGCCAGCTGGTTGAACAGCTCGATCAGGATCGAGAAGCCGATGGCCGCGTACAGGTAGCCTTTGGGGATATGGAAGCCCAGGCCTTCGGCGGTGAGGCTGAAACCGATCATCATCAGGAAGCCCAGGCACAGCATGATCACCGTGGGGTGGGCGTTGACGAAGCGGGTCAG  >  NC_002947/3894244‑3894536

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑