Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 305,153 T→G 8.6% intergenic (+11/+18) hslR → / ← PP_0251 heat shock protein Hsp15/membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947305,1530TG 8.6% 168.4 / 8.1 58intergenic (+11/+18)hslR/PP_0251heat shock protein Hsp15/membrane protein
Reads supporting (aligned to +/- strand):  ref base T (25/28);  new base G (2/3);  total (27/31)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.89e-01

GAAACCGAAGAAAGTGTGCGTCGCCGTGAACAGGCGGCTGAAATGCGTAAGGCCGGGGCCATGGGCGTGACCACCGATGGCCGGCCGACCAAGAAGCAGCGGCGGCAGATTCACCAGCTGCATGGAAGTTACGATTGAATGGGGCTGCTGCGCAGCCCCAAAAAATCTAGCGCAACACCGCCATTCGTCCGACCAACGGTAACGTGGACAGCAGCTTGAACAACGGCGCTGTCCAGCGCATCAACGCCTCGCTGCCTTTGGCCGCCAGCGGCGTGTAGTAGCTCCAGCCCAGCGC  >  NC_002947/305005‑305299
                                                                                                                                                    |                                                                                                                                                  
gAAACCGAAGAAAGTGTGCGTCGCCGTGAACAGGCGGCTGAAATGCGTAAGGCCGGGGCCATGGGCGTGACCACCGATGGCCGGCCGACCAAGAAGCAGCGGCGGCAGATTCACCAGCTGCATGGAAGTTACGATTGAATGGGgctgct                                                                                                                                                    <  7:232123/149‑1 (MQ=255)
     cGAAGAAAGTGTGCGTCGCCGTGAACAGGCGGCTGAAATGCGTAAGGCCGGGGCCATGGGCGTGACCACCGATGGCCGGCCGACCAAGAAGCAGCGGCGGCAGATTCACCAGCTGCATGGAAGTTACGATTGAATGGGGCTGCTgcg                                                                                                                                                 >  6:294214/1‑147 (MQ=255)
     cGAAGAAAGTGTGCGTCGCCGTGAACAGGCGGCTGAAATGCGTAAGGCCGGGGCCATGGGCGTGACCACCGATGGCCGGCCGACCAAGAAGCAGCGGCGGCAGATTCACCAGCTGCATGGAAGTTACGATTGAATGGGGCTGCTgcg                                                                                                                                                 >  7:148897/1‑147 (MQ=255)
      gaagaaAGTGTGCGTCGCCGTGAACAGGCGGCTGAAATGCGTAAGGCCGGGGCCATGGGCGTGACCACCGATGGCCGGCCGACCAAGAAGCAGCGGCGGCAGATTCACCAGCTGCATGGAAGTTACGATTGAATGGGGCTGCTGCGCAg                                                                                                                                              >  1:138847/1‑149 (MQ=255)
      gaagaaAGTGTGCGTCGCCGTGAACAGGCGGCTGAAATGCGTAAGGCCGGGGCCATGGGCGTGACCACCGATGGCCGGCCGACCAAGAAGCAGCGGCGGCAGATTCACCAGCTGCATGGAAGTTACGATTGAATGGGGCTGCTGCGCAg                                                                                                                                              >  7:38432/1‑149 (MQ=255)
         gaaAGTGTGCGTCGCCGTGAACAGGCGGCTGAAATGCGTAAGGCCGGGGCCATGGGCGTGACCACCGATGGCCGGCCGACCAAGAAGCAGCGGCGGCAGATTCACCAGCTGCATGGAAGTTACGATTGAATGGGGCTGCTGCGCAGccc                                                                                                                                           <  6:240887/149‑1 (MQ=255)
             gtgtGCGTCGCCGTGAACAGGCGGCTGAAATGCGTAAGGCCGGGGCCATGGGCGTGACCACCGATGGCCGGCCGACCAAGAAGCAGCGGCGGCAGATTCACCAGCTGCATGGAAGTTACGATTGAATGGGGCTGCGCAGCAGCCCCatt                                                                                                                                       <  3:164033/149‑3 (MQ=255)
                tgCGTCGCCGTGAACAGGCGGCTGAAATGCGTAAGGCCGGGGCCATGGGCGTGTCCACCGATGGCCGGCCGACCAAGAAGCAGCGGCGGCAGATTCACCAGCTGCATGGAAGTTACGATTGAATGGGGCTGCTGCGCAGCCCCaaaaaa                                                                                                                                    <  6:207548/149‑1 (MQ=255)
                 gCGTCGCCGTGAACAGGCGGCTGAAATGCGTAAGGCCGGGGCCATGGGCGTGACCACCGATGGCCGGCCGACCAAGAAGCAGCGGCGGCAGATTCACCAGCTGCATGGAAGTTACGATTGAATGGGGCTGCTGCGCAGCCCCAAAAAAt                                                                                                                                   <  7:291941/149‑1 (MQ=255)
                       ccGTGAACAGGCGGCTGAAATGCGTAAGGCCGGGGCCATGGGCGTGACCACCGATGGCCGGCCGACCAAGAAGCAGCGGCGGCAGATTCACCAGCTGCATGGAAGTTACGATTGAATGGGGCTGCGCAGCAGCCCCa                                                                                                                                         >  3:289555/1‑137 (MQ=255)
                       ccGTGAACAGGCGGCTGAAATGCGTAAGGCCGGGGCCATGGGCGTGACCACCGATGGCCGGCCGACCAAGAAGCAGCGGCGGCAGATTCACCAGCTGCATGGAAGTTACGATTGAATGGGGCTGCGCAGCAGCCCCa                                                                                                                                         <  4:289555/137‑1 (MQ=255)
                        cGTGAGCAGGCGGCTGAAATGCGTAAGGCCGGGGCCATGGGCGTGACCACCGATGGCCGGCCGACCAAGAAGCAGCGGCGGCAGATTCACCAGCTGCATGGAAGTTACGATTGAATGGGGCTGCTGCGCAGCCCCAAAAAATCTAgcg                                                                                                                             >  6:279948/1‑148 (MQ=255)
                        cGTGAACAGGCGGCTGAAATGCGTAAGGCCGGGGCCATGGGCGTGACCACCGATGGCCGGCCGACCAAGAAGCAGCGGCGGCAGATTCACCAGCTGCATGGAAGTTACGATTGAATGGGGCTGCTGCGCAGCCCCAAAAAATCTAgcgc                                                                                                                            <  2:291392/149‑1 (MQ=255)
                        cGTGAACAGGCGGCTGAAATGCGTAAGGCCGGGGCCATGGGCGTGACCACCGATGGCCGGCCGACCAAGAAGCAGCGGCGGCAGATTCACCAGCTGCATGGAAGTTACGATTGAATGGGGCTGCTGCGCAGCCCCAAAAAATCTAg                                                                                                                               <  2:161934/146‑1 (MQ=255)
                        cGTGAACAGGCGGCTGAAATGCGTAAGGCCGGGGCCATGGGCGTGACCACCGATGGCCGGCCGACCAAGAAGCAGCGGCGGCAGATTCACCAGCTGCATGGAAGTTACGATTGAATGGGGCTGCTGCGCAGCCCCAAAAAATCTAg                                                                                                                               >  1:161934/1‑146 (MQ=255)
                                     cTGAAATGCGTAAGGCCGGGGCCATGGGCGTGACCACCGATGGCCGGCCGACCAAGAAGCAGCGGCGGCAGATTCACCAGCTGCAAGGAAGTTACGATTGAATGGGGCTGCTGCGCAGCCCCAAAAAATCTAGCGCAACACCGCCATTc                                                                                                               >  5:146870/1‑149 (MQ=255)
                                       gAAATGCGTAAGGCCGGGGCCATGGGCGTGACCACCGATGGCCGGCCGACCAAGAAGCAGCGGCGGCAGATTCACCAGCTGCATGGAAGTTACGATTGAATGGGGCTGCGCAGCAGCCCCatt                                                                                                                                       <  5:185886/123‑3 (MQ=255)
                                       gAAATGCGTAAGGCCGGGGCCATGGGCGTGACCACCGATGGCCGGCCGACCAAGAAGCAGCGGCGGCAGATTCACCAGCTGCATGGAAGTTACGATTGAATGGGGCTGCGCAGCAGCCCCatt                                                                                                                                       >  6:185886/1‑121 (MQ=255)
                                        aaaTGCGTAAGGCCGGGGCCATGGGCGTGACCACCGATGGCCGGCCGACCAAGAAGCAGCGGCGGCAGATTCACCAGCTGCATGGAAGTTACGATTGAATGGGGCTGCTGCGCAGCCCCAAAAAATCTAGCGCAACACCGCCATTCGTc                                                                                                            >  3:147530/1‑149 (MQ=255)
                                           tGCGTAAGGCCGGGGCCATGGGCGTGACCACCGATGGCCGGCCGACCAAGAAGCAGCGGCGGCAGATTCACCAGCTGCATGGAAGTTACGATTGAATGGGGCTGCTGCGCAGCCCCAAAAAATCTAGCGCAACACCGCCATTCGTCCGa                                                                                                         <  4:147530/149‑1 (MQ=255)
                                               tAAGGCCGGGGCCATGGGCGTGACCACCGATGGCCGGCCGACCAAGAAGCAGCGGCGGCAGATTCACCAGCTGCATGGAAGTTACGATTGAATGGGGCTGCTGCGCAGCCCCAAAAAATCTAGCGCAACACCGCCATTCGTCCGACCaa                                                                                                     <  5:279948/149‑1 (MQ=255)
                                                aaGGCCGGGGCCATGGGCGTGACCACCGATGGCCGGCCGACCAAGAAGCAGCGGCGGCAGATTCACCAGCTGCATGGAAGTTACGATTGAATGGGGCTGCTGCGCAGCCCCAAAAAATCTAGCGCAACACCGCCATTCGTCCGACCAAc                                                                                                    >  5:169118/1‑149 (MQ=255)
                                                 aGGCCGGGGCCATGGGCGTGACCACCGATGGCCGGCCGACGAAGAAGCAGCGGCGGCAGATTCACCAGCTGCATGGAAGTTACGATTGAATGGGGCTGCTGCGCAGCCCCAAAAAATCTAGCGCAACACCGCCATTCGTCCGACCAAc                                                                                                    <  5:294214/148‑1 (MQ=255)
                                                   gCCGGGGCCATGGGCGTGACCACCGATGGCCGGCCGACCAAGAAGCAGCGGCGGCAGATTCACCAGCTGCATGGAAGTTACGATTGAATGGGGCTGCTGCGCAGCCCCAAAAAATCTAGCGCAACACCGCCATTCGTCCGACCAACgg                                                                                                  >  5:196087/1‑148 (MQ=255)
                                                      ggggCCATGGGCGTGACCACCGATGGCCGGCCGACCAAGAAGCAGCGGCGGCAGATTCACCAGCTGCATGGAAGTTACGATTGAATGGGGCTGCTGCGCAGCCCCAAAAAATCTAGCGCAACACCGCCATTCGTCCGACCAACGGTaa                                                                                               <  7:248126/148‑1 (MQ=255)
                                                      ggggCCATGGGCGTGACCACCGATGGCCGGCCGACCAAGAAGCAGCGGCGGCAGATTCACCAGCTGCATGGAAGTTACGATTGAATGGGGCTGCTGCGCAGCCCCAAAAAATCTAGCGCAACACCGCCATTCGTCCGACCAACGGTaa                                                                                               >  8:248126/1‑148 (MQ=255)
                                                           cATGGGCGTGACCACCGATGGCCGGCCGACCAAGAAGCAGCGGCGGCAGATTCACCAGCTGCATGGAAGTTACGATTGAATGGGGCTGCTGCGCAGCCCCAAAAAATCTAGCGCAACACCGCCATTCGTCCGACCAACGGTAACGTGGa                                                                                         <  1:37157/149‑1 (MQ=255)
                                                             tGGGCGTGACCACCGATGGCCGGCCGACCAAGAAGCAGCGGCGGCAGATTCACCAGCTGCATGGAAGTTACGATTGAATGGGGCTGCTGCGCAGCCCCAAAAAATCTAGCGCAACACCGCCATTCGTCCGACCAACGGTAACGTGGa                                                                                         >  7:241994/1‑147 (MQ=255)
                                                               ggCGTGACCACCGATGGCCGGCCGACCAAGAAGCAGCGGCGGCAGATTCACCAGCTGCATGGAAGTTACGATTGAATGGGGCTGCTGCGCAGCCCCAAAAAATCTAGCGCAACACCGCCATTCGTCCGACCAACGGTAACGTGGAcag                                                                                      <  6:196087/148‑1 (MQ=255)
                                                                 cGTGACCACCGATGGCCGGCCGACCAAGAAGCAGCGGCGGCAGATTCACCAGCTGCATGGAAGTTACGATTGAATGGGGCTGCTGCGCAGCCCCAAAAAATCTAGCGCAACACCGCCATTCGTcc                                                                                                           >  5:139172/1‑125 (MQ=255)
                                                                 cGTGACCACCGATGGCCGGCCGACCAAGAAGCAGCGGCGGCAGATTCACCAGCTGCATGGAAGTTACGATTGAATGGGGCTGCTGCGCAGCCCCAAAAAATCTAGCGCAACACCGCCATTCGTcc                                                                                                           <  6:139172/125‑1 (MQ=255)
                                                                     accaccGATGGCCGGCCGACCAAGAAGCAGCGGCGGCAGATTCACCAGCTGCATGGAAGTTACGATTGAATGGGGCTGCTGCGCAGCCCCAAAAAATCTAGCGCAACACCGCCATTCGTCCGACCAACGGTAACGTGGACAGCAGCTTg                                                                               <  8:38432/149‑1 (MQ=255)
                                                                       caccGATGGCCGGCCGACCAAGAAGCAGCGGCGGCAGATTCACCAGCTGCATGGAAGTTACGATTGAATGGGGCTGCTGCGCAGCCCCAAAAAATCTAGCGCAACACCGCCATTCGTCCGACCAACGGTAACGTGGACAGCAGCTTGaa                                                                             >  1:321777/1‑149 (MQ=255)
                                                                        accGATGGCCGGCCGACCAAGAAGCAGCGGCGGCAGATTCACCAGCTGCATGGAAGTTACGATTGAATGGGGCTGCTGCGCAGCCCCAAAAAATCTAGCGCAACACCGCCATTCGTCCGACCAACGGTAACGTGGACAGCAGCTTGaac                                                                            <  2:252550/149‑1 (MQ=255)
                                                                                 cggccgACCAAGAAGCAGCGGCGGCAGATTCACCAGCTGCATGGAAGTTACGATTGAATGGGGCTGCTGCGCAGCCCCAAAAAATCTAGCGCAACACCGCCATTCGTCCGACCAACGGTAACGTGGACAGCAGCTTGAACAACGGCGCt                                                                   >  8:151408/1‑149 (MQ=255)
                                                                                    ccgACCAAGAAGCAGCGGCGGCAGATTCACCAGCTGCATGGAAGTTACGATTGAATGGGGCTGCTGCGCAGCCCCAAAAAATCTAGCGCAACACCGCCATTCGTCCGACCAACGGTAACGTGGACAGCAGCTTGAACAACGGCGCTGTc                                                                >  1:159279/1‑149 (MQ=255)
                                                                                      gACCAAGAAGCAGCGGCGGCAGATTCACCAGCTGCATGGAAGTTACGATTGAATGGGGCTGCTGCGCAGCCCCAAAAAATCTAGCGCAACACCGCCATTCGTCCGACCAACGGTAACGTGGACAGCAGCTTGAACAACGGCGCTGTCCa                                                              <  2:138847/149‑1 (MQ=255)
                                                                                        ccAAGAAGCAGCCGCGGCAGATTCACCAGCTGCATGGAAGTTACGATTGAATGGGGCTGCTGCGCAGCCCCAAAAAATCTAGCGCAACACCGCCATTCGTCCGACCAACGGTAACGTGGACAGCAGCTTGAACAACGGCGCTGTCCAgc                                                            <  8:148897/149‑1 (MQ=255)
                                                                                         cAAGAAGCAGCGGCGGCAGATTCACCAGCTGCATGGAAGTTACGATTGAATGGGGCTGCTGCGCAGCCCCAAAAAATCTAGCGCAACACCGCCATTCGTCCGACCAACGGTAACGTGGACAGCAGCTTGaa                                                                             >  4:67312/1‑131 (MQ=255)
                                                                                         cAAGAAGCAGCGGCGGCAGATTCACCAGCTGCATGGAAGTTACGATTGAATGGGGCTGCTGCGCAGCCCCAAAAAATCTAGCGCAACACCGCCATTCGTCCGACCAACGGTAACGTGGACAGCAGCTTGaa                                                                             <  3:67312/131‑1 (MQ=255)
                                                                                         cAAGAAGCAGCGGCGGCAGATTCACCAGCTGCATGGAAGTTACGATTGAATGGGGCTGCTGCGCAGCCCCAAAAAATCTAGCGCAACACCGCCATTCGTCCGACCAACGGTAACGTGGACAGCAGCTTGAACAACGGCGCTGTCCAgcg                                                           >  4:199427/1‑149 (MQ=255)
                                                                                         cAAGAAGCAGCGGCGGCAGATTCACCAGCTGCATGGAAGTTACGATTGAATGGGGCTGCTGCGCAGCCCCAAAAAATCTAGCGCAACACCGCCATTCGTCCGACCAACGGTAACGTGGACAGCAGCTTGAACAACGGCGCTGTCCAgcg                                                           >  3:53507/1‑149 (MQ=255)
                                                                                            gaagCAGCGGCGGCAGATTCACCAGCTGCATGGAAGTTACGATTGAATGGGGCTGCTGCGCAGCCCCAAAAAATCTAGCGCGACACCGCCATTCGTCCGACCAACGGTAACGTGGACAGCAGCTTGAACAACGGCGCTGTCCAGCGCAt                                                        <  5:140661/149‑1 (MQ=255)
                                                                                                cagcGGCGGCAGATTCACCAGCTGCATGGAAGTTACGATTGAATGGGGCTGCTGCGCAGCCCCAAAAAATCTAGCGCAACACCGCCATTCGTCCGACCAACGGTAACGTGGACAGCAGCTTGAACAACGGCGCTGTCCAGCGCATCAAc                                                    <  3:199427/149‑1 (MQ=255)
                                                                                                          aGATTCACCAGCTGCATGGAAGTTACGAATGAATGGGGCTGCTGCGCAGCCCCAAAAAATCTAGCGCAACACCGCCATTCGTCCGACCCACGGTAACGTGGACAGCAGCTTGAACAACGGCGCTGTCCAGCGCATCAACGCCTCGCTGc                                          <  7:84609/149‑1 (MQ=255)
                                                                                                            aTTCACCAGCTGCATGGAAGTTACGATTGAATGGGGCTGCTGCGCAGCCCCAAAAAATCTAGCGCAACACCGCCATTCGTCCGACCAACGGTAACGTGGACAGCAGCTTGAACAACGGCGCTGTCCAGCGCATCAACGCCTCGCTGCCt                                        >  1:159417/1‑149 (MQ=255)
                                                                                                                aCCAGCTGCATGGAAGTTACGATTGAATGGGGCTGCTGCGCAGCCCCAAAAAATCTAGCGCAACACCGCCATTCGTCCGACCAACGGTAACGTGGACAGCAGCTTGAACAACGGCGCTGTCCAGCGCATCAACGCCTCGCTGCCTTTgg                                    <  4:53507/149‑1 (MQ=255)
                                                                                                                  cAGCTGCATGGAAGTTACGATTGAATGGGGCTGCTGCGCAGCCCCAAAAAATCTAGCGCAACACCGCCATTCGTCCGACCAACGGTAACGTGGACAGCAGCTTGAACAACGGCGCTGTCCAGCGCATCAACGCCTCGCTGCCTTTGgcc                                  <  7:151408/149‑1 (MQ=255)
                                                                                                                        cATGGAAGTTACGATTGAATGGGGCTGCTGCGCAGCCCCAAAAAATCTAGCGCAACACCGCCATTCGTCCGACCAACGGTAACGTGGACAGCAGCTTGAACAACGGCGCTGTCCAGCGCATCAACGCCTCGCTGCCTTTGGCCGCCAgc                            <  2:321777/149‑1 (MQ=255)
                                                                                                                         aTGGAAGTTACGATTGAATGGGGCTGCTGCGCAGCCCCAAAAAATCTAGCGCAACACCGCCATTCGTCCGACCAACGGTAACGTGGACAGCAGCTTGAACAACGGCGCTGTCCAGCGCATCAACGCCTCGCTGCCTTTGGCCGCCAgcg                           >  6:130968/1‑149 (MQ=255)
                                                                                                                          tGGAAGTTACGATTGAATGGGGCTGCTGCGCAGCCCCAAAAAATCTAGCGCAACACCGCCATTCGTCCGACCAACGGTAACGTGGACAGCAGCTTGAACAACGGCGCt                                                                   >  7:202104/1‑108 (MQ=255)
                                                                                                                          tGGAAGTTACGATTGAATGGGGCTGCTGCGCAGCCCCAAAAAATCTAGCGCAACACCGCCATTCGTCCGACCAACGGTAACGTGGACAGCAGCTTGAACAACGGCGCt                                                                   <  8:202104/108‑1 (MQ=255)
                                                                                                                               gTTACGATTGAATGGGGCTGCTGCGCAGCCCCAAAAAATCTAGCGCAACACCGCCATTCGTCCGACCAACGGTAACGTGGACAGCAGCTTGAACAACGGCGCTGTCCAGCGCATCAACGCCTCGCTGCCTTTGGCCGCCAGCGGCgtg                      >  3:59234/1‑148 (MQ=255)
                                                                                                                                   cGATTGAATGGGGCTGCTGCGCAGCCCCAAAAAATCTAGCGCAACACCGCCATTCGTCCGACCAACGGTAACGTGGACAGCAGCTTGAACAACGGCGCTGTCCAGCGCATCAACGCCTCGCTGCCTTTGGCCGCCAGCGGCGTgtagta                 <  5:167076/149‑1 (MQ=255)
                                                                                                                                   cGATTGAATGGGGCTGCTGCGCAGCCCCAAAAAATCTAGCGCAACACCGCCATTCGTCCGACCAACGGTAACGTGGACAGCAGCTTGAACAACGGCGCTGTCCAGCGCATCAACGCCTCGCTGCCTTTGGCCGCCAGCGGCGTgtagt                  <  6:169118/148‑1 (MQ=255)
                                                                                                                                     aTTGAATGGGGCTGCTGCGCAGCCCCAAAAAATCTAGCGCAACACCGCCATTCGTCCGACCAACGGTAACGTGGACAGCAGCTTGAACAACGGCGCTGTCCAGCGCATCAACGCCTCGCTGCCTTTGGCCGCCAGCGGCgt                       <  8:55965/141‑1 (MQ=255)
                                                                                                                                     aTTGAATGGGGCTGCTGCGCAGCCCCAAAAAATCTAGCGCAACACCGCCATTCGTCCGACCAACGGTAACGTGGACAGCAGCTTGAACAACGGCGCTGTCCAGCGCATCAACGCCTCGCTGCCTTTGGCCGCCAGCGGCgt                       >  7:55965/1‑141 (MQ=255)
                                                                                                                                        gAATGGGGCTGCTGCGCAGCCCCAAAAAATCTAGCGCAACACCGCCATTCGTCCGACCAACGGTAACGTGGACAGCAGCTTGAACAACGGCGCTGTCCAGCGCATCAACGCCTCGCTGCCTTTGGCCGCCAGCGGCGTGTAGTAGCTc             <  6:146870/148‑1 (MQ=255)
                                                                                                                                          aTGGGGCTGCTGCGCAGCCCCAAAAAATCTAGCGCAACACCGCCATTCGTCCGACCAACGGTAACGTGGACAGCAGCTTGAACAACGGCGCTGTCCAGCGCATCAACGCCTCGCTGCCTTTGGCCGCCAGCGGCGTGTAGTAGCTccag          >  5:298503/1‑149 (MQ=255)
                                                                                                                                               gctgctGCGCAGCCCCAAAAAATCTAGCGCAACACCGCCATTCGTCCGACCAACGGTAACGTGGACAGCAGCTTGAACAACGGCGCTGTCCAGCGCATCAACGCCTCGCTGCCTTTGGCCGCCAGCGGCGTGTAGTAGCTccagcccag     <  2:159279/149‑1 (MQ=255)
                                                                                                                                                    tgcGCAGCCCCAAAAAATCTAGCGCAACACCGCCATTCGTCCGACCAACGGTAACGTGGACAGCAGCTTGAACAACGGCGCTGTCCAGCGCATCAACGCCTCGCTGCCTTTGGCCGCCAGCGGCGTGTAGTAGCTCCAGCCCAgcgc  >  1:300328/1‑147 (MQ=255)
                                                                                                                                                    |                                                                                                                                                  
GAAACCGAAGAAAGTGTGCGTCGCCGTGAACAGGCGGCTGAAATGCGTAAGGCCGGGGCCATGGGCGTGACCACCGATGGCCGGCCGACCAAGAAGCAGCGGCGGCAGATTCACCAGCTGCATGGAAGTTACGATTGAATGGGGCTGCTGCGCAGCCCCAAAAAATCTAGCGCAACACCGCCATTCGTCCGACCAACGGTAACGTGGACAGCAGCTTGAACAACGGCGCTGTCCAGCGCATCAACGCCTCGCTGCCTTTGGCCGCCAGCGGCGTGTAGTAGCTCCAGCCCAGCGC  >  NC_002947/305005‑305299

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑