Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,912,130 A→G 5.4% E159G (GAG→GGG)  PP_3452 → GGDEF domain‑containing protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,912,1300AG 5.4% 224.2 / 2.3 74E159G (GAG→GGG) PP_3452GGDEF domain‑containing protein
Reads supporting (aligned to +/- strand):  ref base A (38/32);  new base G (2/2);  total (40/34)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.06e-01

CGATAGCCCTGCGCCCGGTGTGGTTGGTGCTCGGGGTGTCCACCGTGCTGTATGCCATGTCGGTGCTACCGGTGCGGCGCTGGTCATTCTTCTGCGCCATGGTCATCACCTGGGTGATGCTTAATCCGTTCTACCACACCCGCATCGAGCCCGATGCGCTCGAAGGCGCCATGCTGATCAGCTATGCCGTGTTTCTAAGCGGCCTGGTCAGCTACAGCTACCTGCAGATCCGCAAGGCCAAGCTGCACAATTTCTACCTGTCCAAGGTGTTGCTGGAGCAAGCCTACGT  >  NC_002947/3911983‑3912271
                                                                                                                                                   |                                                                                                                                             
cGATAGCCCTGCGCCCGGTGTGGTTGGTGCTCGGGGTGTCCACCGTGCTGTATGCCATGTCGGTGCTACCGGTGCGGCGCTGGTCATTCTTCTGCGCCATGGTCATCACCTGGGTGATGCTTAATCCGTTCTACCACACCCGCATCGAg                                                                                                                                              <  3:43496/149‑1 (MQ=255)
 gATAGCCCTGCGCCCGGTGTGGTTGGTGCTCGGGGTGTCCACCGTGCTGTATGCCATGTCGGTGCTACCGGTGCGGCGCTGGTCATTCTTCTGCGCCATGGTCATCACCTGGGTGATGCTTAATCCGTTCTACCACACCCGCATCGAGc                                                                                                                                             >  7:266914/1‑149 (MQ=255)
           cgcCCGGTGTGGTTGGTGCTCGGGGTGTCCACCGTGCTGTATGCCATGTCGGTGCTACCGGTGCGGCGCTGGTCATTCTTCTGCGCCATGGTCATCACCTGGGTGATGCTTAATCCGTTCTACCACACCCGCATCGGGCTCGATgcgg                                                                                                                                    <  5:280132/148‑2 (MQ=255)
               cGGTGTGGTTGGTGCTCGGGGTGTCCACCGTGCTGTATGCCATGTCGGTGCTACCGGTGCGGCGCTGGTCATTCTTCTGCGCCATGGTCATCACCTGGGTGATGCTTAATCCGTTCTACCACACCCGCATCGAGCCCGATGCGCTCGaa                                                                                                                               <  5:234055/149‑1 (MQ=255)
                  tgtgGTTGGTGCTCGGGGTGTCCACCGTGCTGTATGCCATGTCGGTGCTACCGGTGCGGCGCTGGTCATTCTTCTGCGCCATGGTCATCACCTGGGTGATGCTTAATCCGTTCTACCACACCCGCATCGAGCCCGATGCGCTCGAAGgc                                                                                                                            <  3:274423/149‑1 (MQ=255)
                                ggggTGTCCACCGTGCTGTATGCCATGTCGGTGCTACCGGTGCGGCGCTGGTCATTCTTCTGCGCCATGGTCATCACCTGGGTGATGCTTAATCCGTTCTACCACACCCGCATCGAGCCCGATGCGCTCGAAGGCGCCATGCTGATc                                                                                                                <  6:170252/147‑1 (MQ=255)
                                     gtCCACCGTGCTGTATGCCATGTCGGTGCTACCGGTGCGGCGCTGGTCATTCTTCTGCGCCATGGTCATCACCTGGGTGATGCTTAATCCGTTCTACCACACCCGCATCGAGCCCGAt                                                                                                                                        <  7:300553/118‑1 (MQ=255)
                                     gtCCACCGTGCTGTATGCCATGTCGGTGCTACCGGTGCGGCGCTGGTCATTCTTCTGCGCCATGGTCATCACCTGGGTGATGCTTAATCCGTTCTACCACACCCGCATCGAGCCCGAt                                                                                                                                        >  8:300553/1‑118 (MQ=255)
                                     gtCCACCGTGCTGTATGCCATGTCGGTGCTACCGGTGCGGCGCTGGTCATTCTTCTGCGCCATGGTCATCACCTGGGTGATGCTTAATCCGTTCTACCACACCCGCATCGAGCCCGATGCGCTCGAAGGCGCCATGCTGATCAGCTATg                                                                                                         >  4:5504/1‑149 (MQ=255)
                                        cACCGTGCTGTATGCCATGTCGGTGCTACCGGTGCGGCGCTGGTCATTCTTCTGCGCCATGGTCATCAACTGGGTGATGCTTAATCCGTTCTACCACACCCGCATCGAGCCCGATGCGCTCGAAGGCGCCATGCTGATCAGCTATGCCg                                                                                                      >  6:63640/1‑149 (MQ=255)
                                         aCCGTGCTGTATGCCATGTCGGTGCTACCGGTGCGGCGCTGGTCATTCTTCTGCGCCATGGTCATCACCTGGGTGATGCTTAATCCGTTCTACCACACCCGCATCGAGCCCGATGCGCTCGAAGGCGCCATGCTGATCAGCTATGCCgt                                                                                                     >  4:214180/1‑149 (MQ=255)
                                           cGTGCTGTATGCCATGTCGGTGCTACCGGTGCGGCGCTGGTCATTCTTCTGCGCCATGGTCATCACCTGGGTGATGCTTAATCCGTTCTACCACACCCGCATCGAGCCCGATGCGCTCGAAGGCGCCATGc                                                                                                                     >  2:337522/1‑131 (MQ=255)
                                               cTGTATGCCATGTCGGTGCTACCGGTGCGGCGCTGGTCATTCTTCTGCGCCATGGTCATCACCTGGGTGATGCTTAATCCGTTCTACCACACCCGCATCGAGCCCGATGCGCTCGAAGGCGCCATGCTGATCAGCTATGCCGTGTTTc                                                                                                >  6:41383/1‑148 (MQ=255)
                                                  tATGCCATGTCGGTGCTACCGGTGCGGCGCTGGTCATTCTTCTGCGCCATGGTCATCACCTGGGTGATGCTTAATCCGTTCTACCACATCCGCATCGAGCCCGATGCGCTCGAAGGCGCCATGCTGATCAGCTATg                                                                                                         <  1:249606/136‑1 (MQ=255)
                                                  tATGCCATGTCGGTGCTACCGGTGCGGCGCTGGTCATTCTTCTGCGCCATGGTCATCACCTGGGTGATGCTTAATCCGTTCTACCACACCCGCATCGAGCCCGATGCGCTCGAAGGCGCCATGCTGATCAGCTATg                                                                                                         >  2:249606/1‑136 (MQ=255)
                                                      ccATGTCGGTGCTACTGGTGCGGCGCTGGTCATTCTTCTGCGCCATGGTCATCACCTGGGTGATGCTTAATCCGTTCTACCACACCCGCATCGAGCCCGATGCGCTCGAAGGCGCCATGCTGATCAGCTATGCCGTGTTTCTAAGCGGc                                                                                        >  7:76582/1‑149 (MQ=255)
                                                      ccATGTCGGTGCTACCGGTGCGGCGCTGGTCATTCTTCTGCGCCATGGTCATCACCTGGGTGATGCTTAATCCGTTCTACCACACCCGCATCGAGCCCGATGCGCTCGAAGGCGCCATGCTGATCAGCTATGCCGTGTTTCTAAGCGGc                                                                                        >  4:34129/1‑149 (MQ=255)
                                                           tCGGTGCTACCGGTGCGGCGCTGGTCATTCTTCTGCGCCATGGTCATCACCTGGGTGATGCTTAATCCGTTCTACCACACCCGCATCGAGCCCGATGCGCTCGAAGGCGCCATGCTGATCAGCTATGCCGTGTTTCTAAGCGGCCTGGt                                                                                   >  1:228539/1‑149 (MQ=255)
                                                             ggTGCTACCGGTGCGGCGCTGGTCATTCTTCTGCGCCATGGTCATCACCTGGGTGATGCTTAATCCGTTCTACCACACCCGCATCGAGCCCGATGCGCTCGAAGGCGCCATGCTGATCAGCTATGCCGTGTTTCTAAGCGGCCTGGt                                                                                   >  5:62169/1‑147 (MQ=255)
                                                              gTGCTACCGGTGCGGCGCTGGTCATTCTTCTGCGCCATGGTCATCACCTGGGTGATGCTTAATCCGTTCTACCACACCCGCATCGAGCCCGATGCGCTCGAAGGCGCCATGCTGATCAGCTATGCCGTGTTTCTaa                                                                                             >  8:186874/1‑136 (MQ=255)
                                                              gTGCTACCGGTGCGGCGCTGGTCATTCTTCTGCGCCATGGTCATCACCTGGGTGATGCTTAATCCGTTCTACCACACCCGCATCGAGCCCGATGCGCTCGAAGGCGCCATGCTGATCAGCTATGCCGTGTTTCTaa                                                                                             <  7:186874/136‑1 (MQ=255)
                                                               tGCTACCGGTGCGGCGCTGGTCATTCTTCTGCGCCATGGTCATCACCTGGGTGATGCTTAATCCGTTCTACCACACCCGCATCGAGCCCGATGCGCTCGAAGGCGCCATGCTGATCAGCTATGCCGTGTTTCTAAGCGGCCTGGTcagc                                                                               >  7:89422/1‑149 (MQ=255)
                                                               tGCTACCGGTGCGGCGCTGGTCATTCTTCTGCGCCATGGTCATCACCTGGGTGATGCTTAATCCGTTCTACCACACCCGCATCGAGCCCGATGCGCTCGAAGGCGCCATGCTGATCAGCTATGCCGTGTTTCTAAGCGGCCTGGTcagc                                                                               >  1:298361/1‑149 (MQ=255)
                                                                  tACCGGTGCGGCGCTGGTCATTCTTCTGCGCCATGGTCATCACCTGGGTGATGCTTAATCCGTTCTACCACACCCGCATCGAGCCCGATGCGCTCGAAGGCGCCATGCTGATCAGCTATGCCGTGTTTCTAAGCGGCCTGGTcagcta                                                                             >  6:275848/1‑148 (MQ=255)
                                                                    ccGGTGCGGCGCTGGTCATTCTTCTGCGCCATGGTCATCACCTGGGTGATGCTTAATCCGTTCTACCACACCCGCATCGAGCCCGATGCGCTCGAAGGCGCCATGCTGATCAGCTATGCCGTGTTTCTAAGCGGCCTGGTcagctacag                                                                          >  5:60603/1‑149 (MQ=255)
                                                                    ccGGTGCGGCGCTGGTCATTCTTCTGCGCCATGGTCATCACCTGGGTGATGCTTAATCCGTTCTACCACACCCGCATCGAGCCCGATGCGCTCGAAGGCGCCATGCTGATCAGCTATGCCGTGTTTCTAAGCGGCCTGGTcagctac                                                                            <  3:34129/147‑1 (MQ=255)
                                                                        tGCGGCGCTGGTCATTCTTCTGCGCCATGGTCATCACCTGGGTGATGCTTAATCCGTTCTACCACACCCGCATCGAGCCCGATGCGCTCGAAGGCGCCATGCTGATCAGCTATGCCGTGTTTCTAAGCGGCCTGGTCagctacagctac                                                                      <  3:214180/149‑1 (MQ=255)
                                                                           ggcgCTGGTCATTCTTCTGCGCCATGGTCATCACCTGGGTGATGCTTAATCCGTTCTACCACACCCGCATCGAGCCCGATGCGCTCGAAGGCGCCATGCTGATCAGCTATGCCGTGTTTCTAAGCGGCCTGGTCAGCTACAGCTACCTg                                                                   <  8:266914/149‑1 (MQ=255)
                                                                                  gTCATTCTTCTGCGCCATGGTCATCACCTGGGTGATGCTTAATCCGTTCTACCACACCCGCATCGAGCCCGATGCGCTCGAAGGCGCCATGCTGATCAGCTATGCCGTGTTTCTAAGCGGCCTGGTCAGCTACAGCTACCTGCAGATcc                                                            >  3:196401/1‑149 (MQ=255)
                                                                                  gTCATTCTTCTGCGCCATGGTCATCACCTGGGTGATGCTTAATCCGTTCTACCACACCCGCATCGAGCCCGATGCGCTCGAAGGCGCCATGCTGATCAGCTATGCCGTGTTTCTAAGCGGCCTGGTCAGCTACAGCTACCTGCAGATcc                                                            >  3:136612/1‑149 (MQ=255)
                                                                                     aTTCTTCTGCGCCATGGTCATCACCTGGGTGATGCTTAATCCGTTCTACCACACCCGCATCGAGCCCGATGCGCTCGAAGGCGCCATGCTGATCAGCTATGCCGTGTTTCTAAGCGGCCTGGTCAGCTACAGCTACCTGCAGATCCGc                                                          >  4:88453/1‑148 (MQ=255)
                                                                                     aTTCTTCTGCGCCATGGTCATCACCTGGGTGATGCTTAATCCGTTCTACCACACCCGCATCGAGCCCGATGCGCTCGAAGGCGCCATGCTGATCAGCTATGCCGTGTTTCTAAGCGGCCTGGTCAGCTACAGCTACCTGCAGATCCGc                                                          >  6:237878/1‑148 (MQ=255)
                                                                                        cttctGCGCCATGGTCATCACCTGGGTGATGCTTAATCCGTTCTACCACACCCGCATCGAGGCCGATGCGCTCGAAGGCGCCATGCTGATCAGCTATGCCGTGTTTCTAAGCGGCCTGGTCAGCTACAGCTACCTGCAGATCCGCAAg                                                       <  5:63640/148‑1 (MQ=255)
                                                                                        cttctGCGCCATGGTCATCACCTGGGTGATGCTTAATCCGTTCTACCACACCCGCATCGAGCCCGATGCGCTCGAAGGCGCCATGCTGATCAGCTATGCCGTGTTTCTAAGCGGCCTGGTCAGCTACAGCTACCTGCAGATCCGCAAg                                                       <  8:89422/148‑1 (MQ=255)
                                                                                           ctGCGCCATGGTCCTCACCTGGGTGATGCTTAATCCGTTCTACCACACCCGCATCGAGCCCGATGCGCTCGAAGGCGCCATGCTGATCAGCTATGCCGTGTTTCTAAGCGGCCTGGTCAGCTACAGCTACCTGCAGATCCGCAAg                                                       <  8:222137/145‑1 (MQ=255)
                                                                                                gattaagcatcaCCCAGGTGATGCTTAATCCGTTCTACCACACCCGCATCGAGCCCGATGCGCTCGAAGGCGCCATGCTGATCAGCTATGCCGTGTTTCTAAGCGGCCTGGTCAGCTACAGCTACATGCAGATCCGCAAGGCCAAGCTg                                              >  5:187681/8‑149 (MQ=255)
                                                                                                gattaagcatcaCCCAGGTGATGCTTAATCCGTTCTACCACACCCGCATCGAGCCCGATGCGCTCGAAGGCGCCATGCTGATAAGCTATGCCGTGTTTCTAAGCGGCCTGGTCAGCTACAGCTACCTGCAGATCCGCAAGGCCAAGCTg                                              >  7:173668/8‑149 (MQ=255)
                                                                                                  aTGGTCATCACCTGGGTGATGCTTAATCCGTTCTACCACACCCGCATCGAGCCCGATGCGCTCGAAGGCGCCATGCTGATCAGCTATGCCGTGTTTCTAAGCGGCCTGGTCAGCTACAGCTACCTGCAGATCCGCAAGGCCAAGCTGca                                            >  1:40723/1‑149 (MQ=255)
                                                                                                   tGGTCATCACCTGGGTGATGCTTAATCCGTTCTACCACACCCGCATCGAGCCCGATGCGCTCGAAGGCGCCATGCTGATCAGCTATGCCGTGTTTCTAAGCGGc                                                                                        >  2:44794/1‑104 (MQ=255)
                                                                                                      tcatcaCCTGGGTGATGCTTAATCCGTTCTACCACACCCGCATCGAGCCCGATGCGCTCGAAGGCGCCATGCTGATCAGCTATGCCGTGTTTCTAAGCGGCCTGGTCAGCTACAGCTACCTGCAGATCCGCAAGGCCAAGCTGCACAAt                                        <  2:298361/149‑1 (MQ=255)
                                                                                                       catcaCCTGGGCGATGCTTAATCCGTTCTACCACACCCGCATCGAGCCCGATGCGCTCGAAGGCGCCATGCTGACCAGCTATGCCGTGTTTCTAAGCGGCCTGGTCAGCTACAGCTACCTGCAGATCCGCAAGGCCAAGCTGCACAAtt                                       >  2:152936/1‑149 (MQ=255)
                                                                                                         tcaCCTGGGTGATGCTTAATCCGTTCTACCACACCCGCATCGAGCCCGATGCGCTCGAAGGCGCCATGCTGATCAGCTATGCCGTGTTTCTAAGCGGCCTGGTCAGCTACAGCTACCTGCAGATCCGCAAGGCCAAGCTGCACAATTTc                                     <  5:275848/149‑1 (MQ=255)
                                                                                                          caCCTGGGTGATGCTTAATCCGTTCTACCACACCCGCATCGAGCCCGATGCGCTCGAAGGCGCCATGCTGATCAGCTATGCCGTGTTTCTAAGCGGCCTGGTCAGCTACAGCTACCTGCAGATCCGCAAGGCCAAGCTGCACAATTTc                                     >  6:14685/1‑148 (MQ=255)
                                                                                                          caCCTGGGTGATGCTTAATCCGTTCTACCACACCCGCATCGAGCCCGATGCGCTCGAAGGCGCCATGCTGATCAGCTATGCCGTGTTTCTAAGCGGCCTGGTCAGCTACAGCTACCTGCAGATCCGCAAGGCCAAGCTGCACAATTTc                                     >  7:302469/1‑148 (MQ=255)
                                                                                                           aCCTGGGTGATGCTTAATCCGTTCTACCACACCCGCATCGAGCCCGATGCGCTCGAAGGCGCCATGCTGATCAGCTATGCCGTGTTTCTAAGCGGCCTGGTCAGCTACAGCTACCTGCAGATCCGCAAGGCCAAGCTGCACAATTTCt                                    <  6:60603/148‑1 (MQ=255)
                                                                                                           aCCTGGGTGATGCTTAATCCGTTCTACCACACCCGCATCGAGCCCGATGCGCTCGAAGGCGCCATGCTGATCAGCTATGCCGTGTTTCTAAGCGGCCTGGTCAGCTACAGCTACCTGCAGATCCGCAAGGCCAAGCTGCACAATTTCTa                                   <  5:41383/149‑1 (MQ=255)
                                                                                                           aCCTGGGTGATGCTTAATCCGTTCTACCACACCCGCATCGAGCCCGATGCGCTCGAAGGCGCCATGCTGATCAGCTATGCCGTGTTTCTAAGCGGCCTGGTCAGCTACAGCTACCTGCAGATCCGCAAGGCCAAGCTGCACAATTTCTa                                   >  4:315354/1‑149 (MQ=255)
                                                                                                           aCCTGGGTGATGCTTAATCCGTTCTACCACACCCGCATCGAGCCCGATGCGCTCGAAGGCGCCATGCTGATCAGCTATGCCGTGTTTCTAAGCGGCCTGGTCAGCTACAGCTACCTGCAGATCCGCAAGGCCAAGCTGCACAATTTCTa                                   <  8:302469/149‑1 (MQ=255)
                                                                                                            ccTGGGTGATGCTTAATCCGTTCTACCACACCCGCATCGAGCCCGATGCGCTCGAAGGCGCCATGCTGATCAGCTATGCCGTGTTTCTAAGCGGCCTGGTCAGCTACAGCTACCTGCAGATCCGCAAGGCCAAGCTGCACAATTTCTAc                                  <  5:237878/149‑1 (MQ=255)
                                                                                                            ccTGGGTGATGCTTAATCCGTTCTACCACACCCGCATCGAGCCCGATGCGCTCGAAGGCGCCATGCTGATCAGCTATGCCGTGTTTCTAAGCGGCCTGGTCAGCTACAGCTACCTGCAGATCCGCAAGGCCAAGCTGCACAATTTCTAc                                  <  3:24901/149‑1 (MQ=255)
                                                                                                             cTGGGTGATGCTTAATCCGTTCTACCACACCCGCATCGAGCCCGATGCGCTCGAAGGCGCCATGCTGATCAGCTATGCCGTGTTTCTAAGCGGCCTGGTCAGCTACAGCTACCTGCAGATCCGCAAGGCCAAGCTGCACAATTTCTAcc                                 >  1:174771/1‑149 (MQ=255)
                                                                                                                ggTGATGCTTAATCCGTTCTACCACACCCGCATCGAGCCCGATGCGCTCGAAGGCGCCATGCTGATCAGCTATGCCGTGTTTCTAAGCGGCCTGGTCAGCTACAGCTACCTGCAGATCCGCAAGGCCAAGCTGCACAATTTCTACCTGt                              >  6:269448/1‑149 (MQ=255)
                                                                                                                ggTGATGCTTAATCCGTTCTACCACACCCGCATCGAGCCCGATGCGCTCGAAGGCGCCATGCTGATCAGCTATGCCGTGTTTCTAAGCGGCCTGGTCAGCTACAGCTACCTGCAGATCCGCAAGGCCAAGCTGCACAATTTCTACCTGt                              >  3:284393/1‑149 (MQ=255)
                                                                                                                ggTGATGCTTAATCCGTTCTACCACACCCGCATCGAGCCCGATGCGCTCGAAGGCGCCATGCTGATCAGCTATGCCGTGTTTCTAAGCGGCCTGGTCAGCTACAGCTACCTGCAGATCCGCAAGGCCAAGCTGCACAATTTCTACCTGt                              <  3:5504/149‑1 (MQ=255)
                                                                                                                     tGCTTAATCCGTTCTACCACACCCGCATCGAGCCCGATGCGCTCGAAGGCGCCATGCTGATCAGCTATGCCGTGTTTCTAAGCGGCCTGGTCAGCTACAGCTACCTGCAGATCCGCAAGGCCAAGCTGCACAATTTCTACCTGTCCAAg                         <  2:228539/149‑1 (MQ=255)
                                                                                                                           aTCCGTTCTACCACACCCGCATCGAGCCCGATGCGCTCGAAGGCGCCATGCTGATCAGCTATGCCGTGTTTCTAAGCGGCCTGGTCAGCTACAGCTACCTGCAGATCCGCAAGGCCAAGCTGCACAATTTCTACCTGTCCAAGGTGTTg                   <  4:136612/149‑1 (MQ=255)
                                                                                                                              cGTTCTACCACACCCGCATCGAGCCCGATGCGCTCGAAGGCGCCATGCTGATCAGCTATGCCGTGTTTCTAAGCGGCCTGGTCAGCTACAGCTACCTGCAGATCCGCAAGGCCAAGCTGCACAATTTCTACCTGTCCAAGGTGTTGCTg                <  2:40723/149‑1 (MQ=255)
                                                                                                                              cGTTCTACCACACCCGCATCGAGACCGATTCGCTCGAAGGCGCCATGCTGATCAGCTATGCCGTGTTTCTAAGCGGCCTGGTCAGCTACAGCTACCTGCAGATCCGCAAGGCCAAGCTGCACAATTTCTACATGTCCAAGGTGTTGCTg                >  3:180575/1‑149 (MQ=255)
                                                                                                                                ttCTACCACACCCGCATCGAGCCCGATGCGCTCGAAGGCGCCATGCTGATCAGCTATGCCGTGTTTCTAAGCGGCCTGGTCAGCTACAGCTACCTGCAGATc                                                             >  1:185522/1‑102 (MQ=255)
                                                                                                                                ttCTACCACACCCGCATCGAGCCCGATGCGCTCGAAGGCGCCATGCTGATCAGCTATGCCGTGTTTCTAAGCGGCCTGGTCAGCTACAGCTACCTGCAGATc                                                             <  2:185522/102‑1 (MQ=255)
                                                                                                                                ttCTACCACACCCGCATCGAGCCCGATGCGCTCGAAGGCGCCATGCTGATCAGCTATGCCGTGTTTCTAAGCGGCCTGGTCAGCTACAGCTACCTGCAGATCCGCAAGGCCAAGCTGCACAATTTCTACCTGTCCAAGGTGTTGCTGGa              <  6:62169/149‑1 (MQ=255)
                                                                                                                                   tACCACACCCGCATCGAGCCCGATGCGCTCGAAGGCGCCATGCTGATCAGCTATGCCGTGTTTCTAAGCGGCCTGGTCAGCTACAGCTACCTGCAGATCCGCAAGGCCAAGCTGCACAATTTCTACCTGTCCAAGGTGTTGCTGGAGc            >  6:236950/1‑148 (MQ=255)
                                                                                                                                     ccACACCCGCATCGAGCCCGATGCGCTCGAAGGCGCCATGCTGATCAGCTATGCCGTGTTTCTAAGCGGCCTGGTCAGCTACAGCTACCTGCAGATCCGCAAGGCCAAGCTGCACAATTTCTACCTGTCCAATGTGTTGCTGGAGc            <  5:236950/146‑1 (MQ=255)
                                                                                                                                     ccACACCCGCATCGAGCCCGATGCGCTCGAAGGCGCCATGCTGATCAGCTATGCCGTGTTTCTAAGCGGCCTGGTCAGCTACAGCTACCTGCAGATCCGCAAGGCCAAGCTGCACAATTTCTACCTGTCCAAGGTGTTGCTGGAGCaa          <  5:269448/148‑1 (MQ=255)
                                                                                                                                     ccACACCCACATCGAGCCCGATGCGCTCGAAGGCGCCATGCTGATCAGCTATGCCGTGTTTCTAAGCGGCCTGGTCAGCTACAGCTACCTGCAGATCCGCAAGGCCAAGCTGCACAATTTCTACCTGTCCAAGGTGTTGCTGGAGCAAg         >  4:103181/1‑149 (MQ=255)
                                                                                                                                      cacaCCCGCATCGAGCCCGATGCGCTCGAAGGCGCCATGCTGATCAGCTATGCCGTGTTTCTAAGCGGCCTGGTCAGCTACAGCTACCTGCAGATCCGCAAGGCCAAGCTGCACAATTTCTACCTGTCCAAGGTGTTGCTGGAGCAAGc        <  4:284393/149‑1 (MQ=255)
                                                                                                                                        cacCCGCATCGAGCCCGATGCGCTCGAAGGCGCCATGCTGATCAGCTATGCCGTGTTTCTAAGCGGCCTGGTCAGCTACAGCTACCTGCAGATCCGCAAGGCCAAGCTGCACAATTTCTACCTGTCCAAGGTGTTGCTGGAGCAAGCCt      <  8:76582/149‑1 (MQ=255)
                                                                                                                                          agcGCATCGGGCTCGATGCGCTCGAAGGCGCCATGCTGATCAGCTATGCCGTGTTTCTAAGCGGCCTGGTCAGCTACAGCTACCTGCAGATCCGCAAGGCCAAGCTGCACAATTTCTACCTGTCCAAGGTGTTGCTGGAGCAAGCCTAc    >  6:130997/3‑149 (MQ=255)
                                                                                                                                           gcGCATCGGGCTCGATGCGCTCGAAGGCGCCATGCTGATCAGCTATGCCGTGTTTCTAAGCGGCCTGGTCAGCTACAGCTACCTGCAGATCCGCAAGGCCAAGCTGCACAATTTCTACCTGTCCAAGGTGTTGCTGGAGCAAGCCTACg   <  5:130997/148‑1 (MQ=255)
                                                                                                                                           gcGCATCGGGCTCGATGCGCTCGAAGGCGCCATGCTGATCAGCTATGCCGTGTTTCTAAGCGGCCTGGTCAGCTACAGCTACCTGCAGAACCGCAAGGCCAAGCTGCACAATTTCTACCTGTCCAAGGTGTTGCTGGAGCAAGCCTACg   >  6:119787/2‑149 (MQ=255)
                                                                                                                                           ccGCATCGAGCCCGATGCGCTCGAAGGCGCCATGCTGATCAGCTATGCCGTGTTTCTAAGCGGCCTGGTCAGCTACAGCTACCTGCAGATCCGCAAGGCCAAGCTGCACAATTTCTACCTGTCCAAGGTGTTGCTGGAGCAAGCCTACg   >  5:71835/1‑149 (MQ=255)
                                                                                                                                            cGCATCGAGCCCGATGCGCTCGAAGGCGCCATGCTGATCAGCTATGCCGTGTTTCTAAGCGGCCTGGTCAGCTACAGCTACCTGCAGATCCGCAAGGCCAAGCTGCACAATTTCTACCTGTCCAAGGTGTTGCTGGAGCAAGCCTACGt  <  2:174771/149‑1 (MQ=255)
                                                                                                                                                  gAGCCCGATGCGCTCGAAGGCGCCATGCTGATCAGCTATGCCGTGTTTCTAAGCGGCCTGGTCAGCTACAGCTACCTGCAGATc                                                             <  7:164969/84‑1 (MQ=255)
                                                                                                                                                  gAGCCCGATGCGCTCGAAGGCGCCATGCTGATCAGCTATGCCGTGTTTCTAAGCGGCCTGGTCAGCTACAGCTACCTGCAGATc                                                             >  8:164969/1‑84 (MQ=255)
                                                                                                                                                   |                                                                                                                                             
CGATAGCCCTGCGCCCGGTGTGGTTGGTGCTCGGGGTGTCCACCGTGCTGTATGCCATGTCGGTGCTACCGGTGCGGCGCTGGTCATTCTTCTGCGCCATGGTCATCACCTGGGTGATGCTTAATCCGTTCTACCACACCCGCATCGAGCCCGATGCGCTCGAAGGCGCCATGCTGATCAGCTATGCCGTGTTTCTAAGCGGCCTGGTCAGCTACAGCTACCTGCAGATCCGCAAGGCCAAGCTGCACAATTTCTACCTGTCCAAGGTGTTGCTGGAGCAAGCCTACGT  >  NC_002947/3911983‑3912271

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑