| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 305,155 | C→A | 8.8% | intergenic (+13/+16) | hslR → / ← PP_0251 | heat shock protein Hsp15/membrane protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 305,155 | 0 | C | A | 8.8% | 179.5 / 6.0 | 57 | intergenic (+13/+16) | hslR/PP_0251 | heat shock protein Hsp15/membrane protein |
| Reads supporting (aligned to +/- strand): ref base C (23/29); new base A (2/3); total (25/32) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.87e-01 | |||||||||||
CGAAGAAAGTGTGCGTCGCCGTGAACAGGCGGCTGAAATGCGTAAGGCCGGGGCCATGGGCGTGACCACCGATGGCCGGCCGACCAAGAAGCAGCGGCGGCAGATTCACCAGCTGCATGGAAGTTACGATTGAATGGGGCTGCTGCGCAGCCCCAAAAAATCTAGCGCAACACCGCCATTCGTCCGACCAACGGTAACGTGGACAGCAGCTTGAACAACGGCGCTGTCCAGCGCATCAACGCCTCGCTGCCTTTGGCCGCCAGCGGCGTGTAGTAGCTCCAGCCCAGCGCCA > NC_002947/305010‑305301 | cGAAGAAAGTGTGCGTCGCCGTGAACAGGCGGCTGAAATGCGTAAGGCCGGGGCCATGGGCGTGACCACCGATGGCCGGCCGACCAAGAAGCAGCGGCGGCAGATTCACCAGCTGCATGGAAGTTACGATTGAATGGGGCTGCTgcg > 6:294214/1‑147 (MQ=255)cGAAGAAAGTGTGCGTCGCCGTGAACAGGCGGCTGAAATGCGTAAGGCCGGGGCCATGGGCGTGACCACCGATGGCCGGCCGACCAAGAAGCAGCGGCGGCAGATTCACCAGCTGCATGGAAGTTACGATTGAATGGGGCTGCTgcg > 7:148897/1‑147 (MQ=255) gaagaaAGTGTGCGTCGCCGTGAACAGGCGGCTGAAATGCGTAAGGCCGGGGCCATGGGCGTGACCACCGATGGCCGGCCGACCAAGAAGCAGCGGCGGCAGATTCACCAGCTGCATGGAAGTTACGATTGAATGGGGCTGCTGCGCAg > 1:138847/1‑149 (MQ=255) gaagaaAGTGTGCGTCGCCGTGAACAGGCGGCTGAAATGCGTAAGGCCGGGGCCATGGGCGTGACCACCGATGGCCGGCCGACCAAGAAGCAGCGGCGGCAGATTCACCAGCTGCATGGAAGTTACGATTGAATGGGGCTGCTGCGCAg > 7:38432/1‑149 (MQ=255) gaaAGTGTGCGTCGCCGTGAACAGGCGGCTGAAATGCGTAAGGCCGGGGCCATGGGCGTGACCACCGATGGCCGGCCGACCAAGAAGCAGCGGCGGCAGATTCACCAGCTGCATGGAAGTTACGATTGAATGGGGCTGCTGCGCAGccc < 6:240887/149‑1 (MQ=255) gtgtGCGTCGCCGTGAACAGGCGGCTGAAATGCGTAAGGCCGGGGCCATGGGCGTGACCACCGATGGCCGGCCGACCAAGAAGCAGCGGCGGCAGATTCACCAGCTGCATGGAAGTTACGATTGAATGGGGCTGCGCAGCAGCCCCatt < 3:164033/149‑3 (MQ=255) tgCGTCGCCGTGAACAGGCGGCTGAAATGCGTAAGGCCGGGGCCATGGGCGTGTCCACCGATGGCCGGCCGACCAAGAAGCAGCGGCGGCAGATTCACCAGCTGCATGGAAGTTACGATTGAATGGGGCTGCTGCGCAGCCCCaaaaaa < 6:207548/149‑1 (MQ=255) gCGTCGCCGTGAACAGGCGGCTGAAATGCGTAAGGCCGGGGCCATGGGCGTGACCACCGATGGCCGGCCGACCAAGAAGCAGCGGCGGCAGATTCACCAGCTGCATGGAAGTTACGATTGAATGGGGCTGCTGCGCAGCCCCAAAAAAt < 7:291941/149‑1 (MQ=255) ccGTGAACAGGCGGCTGAAATGCGTAAGGCCGGGGCCATGGGCGTGACCACCGATGGCCGGCCGACCAAGAAGCAGCGGCGGCAGATTCACCAGCTGCATGGAAGTTACGATTGAATGGGGCTGCGCAGCAGCCCCa > 3:289555/1‑137 (MQ=255) ccGTGAACAGGCGGCTGAAATGCGTAAGGCCGGGGCCATGGGCGTGACCACCGATGGCCGGCCGACCAAGAAGCAGCGGCGGCAGATTCACCAGCTGCATGGAAGTTACGATTGAATGGGGCTGCGCAGCAGCCCCa < 4:289555/137‑1 (MQ=255) cGTGAGCAGGCGGCTGAAATGCGTAAGGCCGGGGCCATGGGCGTGACCACCGATGGCCGGCCGACCAAGAAGCAGCGGCGGCAGATTCACCAGCTGCATGGAAGTTACGATTGAATGGGGCTGCTGCGCAGCCCCAAAAAATCTAgcg > 6:279948/1‑148 (MQ=255) cGTGAACAGGCGGCTGAAATGCGTAAGGCCGGGGCCATGGGCGTGACCACCGATGGCCGGCCGACCAAGAAGCAGCGGCGGCAGATTCACCAGCTGCATGGAAGTTACGATTGAATGGGGCTGCTGCGCAGCCCCAAAAAATCTAgcgc < 2:291392/149‑1 (MQ=255) cGTGAACAGGCGGCTGAAATGCGTAAGGCCGGGGCCATGGGCGTGACCACCGATGGCCGGCCGACCAAGAAGCAGCGGCGGCAGATTCACCAGCTGCATGGAAGTTACGATTGAATGGGGCTGCTGCGCAGCCCCAAAAAATCTAg < 2:161934/146‑1 (MQ=255) cGTGAACAGGCGGCTGAAATGCGTAAGGCCGGGGCCATGGGCGTGACCACCGATGGCCGGCCGACCAAGAAGCAGCGGCGGCAGATTCACCAGCTGCATGGAAGTTACGATTGAATGGGGCTGCTGCGCAGCCCCAAAAAATCTAg > 1:161934/1‑146 (MQ=255) cTGAAATGCGTAAGGCCGGGGCCATGGGCGTGACCACCGATGGCCGGCCGACCAAGAAGCAGCGGCGGCAGATTCACCAGCTGCAAGGAAGTTACGATTGAATGGGGCTGCTGCGCAGCCCCAAAAAATCTAGCGCAACACCGCCATTc > 5:146870/1‑149 (MQ=255) gAAATGCGTAAGGCCGGGGCCATGGGCGTGACCACCGATGGCCGGCCGACCAAGAAGCAGCGGCGGCAGATTCACCAGCTGCATGGAAGTTACGATTGAATGGGGCTGCGCAGCAGCCCCatt < 5:185886/123‑3 (MQ=255) gAAATGCGTAAGGCCGGGGCCATGGGCGTGACCACCGATGGCCGGCCGACCAAGAAGCAGCGGCGGCAGATTCACCAGCTGCATGGAAGTTACGATTGAATGGGGCTGCGCAGCAGCCCCatt > 6:185886/1‑121 (MQ=255) aaaTGCGTAAGGCCGGGGCCATGGGCGTGACCACCGATGGCCGGCCGACCAAGAAGCAGCGGCGGCAGATTCACCAGCTGCATGGAAGTTACGATTGAATGGGGCTGCTGCGCAGCCCCAAAAAATCTAGCGCAACACCGCCATTCGTc > 3:147530/1‑149 (MQ=255) tGCGTAAGGCCGGGGCCATGGGCGTGACCACCGATGGCCGGCCGACCAAGAAGCAGCGGCGGCAGATTCACCAGCTGCATGGAAGTTACGATTGAATGGGGCTGCTGCGCAGCCCCAAAAAATCTAGCGCAACACCGCCATTCGTCCGa < 4:147530/149‑1 (MQ=255) tAAGGCCGGGGCCATGGGCGTGACCACCGATGGCCGGCCGACCAAGAAGCAGCGGCGGCAGATTCACCAGCTGCATGGAAGTTACGATTGAATGGGGCTGCTGCGCAGCCCCAAAAAATCTAGCGCAACACCGCCATTCGTCCGACCaa < 5:279948/149‑1 (MQ=255) aaGGCCGGGGCCATGGGCGTGACCACCGATGGCCGGCCGACCAAGAAGCAGCGGCGGCAGATTCACCAGCTGCATGGAAGTTACGATTGAATGGGGCTGCTGCGCAGCCCCAAAAAATCTAGCGCAACACCGCCATTCGTCCGACCAAc > 5:169118/1‑149 (MQ=255) aGGCCGGGGCCATGGGCGTGACCACCGATGGCCGGCCGACGAAGAAGCAGCGGCGGCAGATTCACCAGCTGCATGGAAGTTACGATTGAATGGGGCTGCTGCGCAGCCCCAAAAAATCTAGCGCAACACCGCCATTCGTCCGACCAAc < 5:294214/148‑1 (MQ=255) gCCGGGGCCATGGGCGTGACCACCGATGGCCGGCCGACCAAGAAGCAGCGGCGGCAGATTCACCAGCTGCATGGAAGTTACGATTGAATGGGGCTGCTGCGCAGCCCCAAAAAATCTAGCGCAACACCGCCATTCGTCCGACCAACgg > 5:196087/1‑148 (MQ=255) ggggCCATGGGCGTGACCACCGATGGCCGGCCGACCAAGAAGCAGCGGCGGCAGATTCACCAGCTGCATGGAAGTTACGATTGAATGGGGCTGCTGCGCAGCCCCAAAAAATCTAGCGCAACACCGCCATTCGTCCGACCAACGGTaa > 8:248126/1‑148 (MQ=255) ggggCCATGGGCGTGACCACCGATGGCCGGCCGACCAAGAAGCAGCGGCGGCAGATTCACCAGCTGCATGGAAGTTACGATTGAATGGGGCTGCTGCGCAGCCCCAAAAAATCTAGCGCAACACCGCCATTCGTCCGACCAACGGTaa < 7:248126/148‑1 (MQ=255) cATGGGCGTGACCACCGATGGCCGGCCGACCAAGAAGCAGCGGCGGCAGATTCACCAGCTGCATGGAAGTTACGATTGAATGGGGCTGCTGCGCAGCCCCAAAAAATCTAGCGCAACACCGCCATTCGTCCGACCAACGGTAACGTGGa < 1:37157/149‑1 (MQ=255) tGGGCGTGACCACCGATGGCCGGCCGACCAAGAAGCAGCGGCGGCAGATTCACCAGCTGCATGGAAGTTACGATTGAATGGGGCTGCTGCGCAGCCCCAAAAAATCTAGCGCAACACCGCCATTCGTCCGACCAACGGTAACGTGGa > 7:241994/1‑147 (MQ=255) ggCGTGACCACCGATGGCCGGCCGACCAAGAAGCAGCGGCGGCAGATTCACCAGCTGCATGGAAGTTACGATTGAATGGGGCTGCTGCGCAGCCCCAAAAAATCTAGCGCAACACCGCCATTCGTCCGACCAACGGTAACGTGGAcag < 6:196087/148‑1 (MQ=255) cGTGACCACCGATGGCCGGCCGACCAAGAAGCAGCGGCGGCAGATTCACCAGCTGCATGGAAGTTACGATTGAATGGGGCTGCTGCGCAGCCCCAAAAAATCTAGCGCAACACCGCCATTCGTcc > 5:139172/1‑125 (MQ=255) cGTGACCACCGATGGCCGGCCGACCAAGAAGCAGCGGCGGCAGATTCACCAGCTGCATGGAAGTTACGATTGAATGGGGCTGCTGCGCAGCCCCAAAAAATCTAGCGCAACACCGCCATTCGTcc < 6:139172/125‑1 (MQ=255) accaccGATGGCCGGCCGACCAAGAAGCAGCGGCGGCAGATTCACCAGCTGCATGGAAGTTACGATTGAATGGGGCTGCTGCGCAGCCCCAAAAAATCTAGCGCAACACCGCCATTCGTCCGACCAACGGTAACGTGGACAGCAGCTTg < 8:38432/149‑1 (MQ=255) caccGATGGCCGGCCGACCAAGAAGCAGCGGCGGCAGATTCACCAGCTGCATGGAAGTTACGATTGAATGGGGCTGCTGCGCAGCCCCAAAAAATCTAGCGCAACACCGCCATTCGTCCGACCAACGGTAACGTGGACAGCAGCTTGaa > 1:321777/1‑149 (MQ=255) accGATGGCCGGCCGACCAAGAAGCAGCGGCGGCAGATTCACCAGCTGCATGGAAGTTACGATTGAATGGGGCTGCTGCGCAGCCCCAAAAAATCTAGCGCAACACCGCCATTCGTCCGACCAACGGTAACGTGGACAGCAGCTTGaac < 2:252550/149‑1 (MQ=255) cggccgACCAAGAAGCAGCGGCGGCAGATTCACCAGCTGCATGGAAGTTACGATTGAATGGGGCTGCTGCGCAGCCCCAAAAAATCTAGCGCAACACCGCCATTCGTCCGACCAACGGTAACGTGGACAGCAGCTTGAACAACGGCGCt > 8:151408/1‑149 (MQ=255) ccgACCAAGAAGCAGCGGCGGCAGATTCACCAGCTGCATGGAAGTTACGATTGAATGGGGCTGCTGCGCAGCCCCAAAAAATCTAGCGCAACACCGCCATTCGTCCGACCAACGGTAACGTGGACAGCAGCTTGAACAACGGCGCTGTc > 1:159279/1‑149 (MQ=255) gACCAAGAAGCAGCGGCGGCAGATTCACCAGCTGCATGGAAGTTACGATTGAATGGGGCTGCTGCGCAGCCCCAAAAAATCTAGCGCAACACCGCCATTCGTCCGACCAACGGTAACGTGGACAGCAGCTTGAACAACGGCGCTGTCCa < 2:138847/149‑1 (MQ=255) ccAAGAAGCAGCCGCGGCAGATTCACCAGCTGCATGGAAGTTACGATTGAATGGGGCTGCTGCGCAGCCCCAAAAAATCTAGCGCAACACCGCCATTCGTCCGACCAACGGTAACGTGGACAGCAGCTTGAACAACGGCGCTGTCCAgc < 8:148897/149‑1 (MQ=255) cAAGAAGCAGCGGCGGCAGATTCACCAGCTGCATGGAAGTTACGATTGAATGGGGCTGCTGCGCAGCCCCAAAAAATCTAGCGCAACACCGCCATTCGTCCGACCAACGGTAACGTGGACAGCAGCTTGaa > 4:67312/1‑131 (MQ=255) cAAGAAGCAGCGGCGGCAGATTCACCAGCTGCATGGAAGTTACGATTGAATGGGGCTGCTGCGCAGCCCCAAAAAATCTAGCGCAACACCGCCATTCGTCCGACCAACGGTAACGTGGACAGCAGCTTGaa < 3:67312/131‑1 (MQ=255) cAAGAAGCAGCGGCGGCAGATTCACCAGCTGCATGGAAGTTACGATTGAATGGGGCTGCTGCGCAGCCCCAAAAAATCTAGCGCAACACCGCCATTCGTCCGACCAACGGTAACGTGGACAGCAGCTTGAACAACGGCGCTGTCCAgcg > 4:199427/1‑149 (MQ=255) cAAGAAGCAGCGGCGGCAGATTCACCAGCTGCATGGAAGTTACGATTGAATGGGGCTGCTGCGCAGCCCCAAAAAATCTAGCGCAACACCGCCATTCGTCCGACCAACGGTAACGTGGACAGCAGCTTGAACAACGGCGCTGTCCAgcg > 3:53507/1‑149 (MQ=255) gaagCAGCGGCGGCAGATTCACCAGCTGCATGGAAGTTACGATTGAATGGGGCTGCTGCGCAGCCCCAAAAAATCTAGCGCGACACCGCCATTCGTCCGACCAACGGTAACGTGGACAGCAGCTTGAACAACGGCGCTGTCCAGCGCAt < 5:140661/149‑1 (MQ=255) cagcGGCGGCAGATTCACCAGCTGCATGGAAGTTACGATTGAATGGGGCTGCTGCGCAGCCCCAAAAAATCTAGCGCAACACCGCCATTCGTCCGACCAACGGTAACGTGGACAGCAGCTTGAACAACGGCGCTGTCCAGCGCATCAAc < 3:199427/149‑1 (MQ=255) aGATTCACCAGCTGCATGGAAGTTACGAATGAATGGGGCTGCTGCGCAGCCCCAAAAAATCTAGCGCAACACCGCCATTCGTCCGACCCACGGTAACGTGGACAGCAGCTTGAACAACGGCGCTGTCCAGCGCATCAACGCCTCGCTGc < 7:84609/149‑1 (MQ=255) aTTCACCAGCTGCATGGAAGTTACGATTGAATGGGGCTGCTGCGCAGCCCCAAAAAATCTAGCGCAACACCGCCATTCGTCCGACCAACGGTAACGTGGACAGCAGCTTGAACAACGGCGCTGTCCAGCGCATCAACGCCTCGCTGCCt > 1:159417/1‑149 (MQ=255) aCCAGCTGCATGGAAGTTACGATTGAATGGGGCTGCTGCGCAGCCCCAAAAAATCTAGCGCAACACCGCCATTCGTCCGACCAACGGTAACGTGGACAGCAGCTTGAACAACGGCGCTGTCCAGCGCATCAACGCCTCGCTGCCTTTgg < 4:53507/149‑1 (MQ=255) cAGCTGCATGGAAGTTACGATTGAATGGGGCTGCTGCGCAGCCCCAAAAAATCTAGCGCAACACCGCCATTCGTCCGACCAACGGTAACGTGGACAGCAGCTTGAACAACGGCGCTGTCCAGCGCATCAACGCCTCGCTGCCTTTGgcc < 7:151408/149‑1 (MQ=255) cATGGAAGTTACGATTGAATGGGGCTGCTGCGCAGCCCCAAAAAATCTAGCGCAACACCGCCATTCGTCCGACCAACGGTAACGTGGACAGCAGCTTGAACAACGGCGCTGTCCAGCGCATCAACGCCTCGCTGCCTTTGGCCGCCAgc < 2:321777/149‑1 (MQ=255) aTGGAAGTTACGATTGAATGGGGCTGCTGCGCAGCCCCAAAAAATCTAGCGCAACACCGCCATTCGTCCGACCAACGGTAACGTGGACAGCAGCTTGAACAACGGCGCTGTCCAGCGCATCAACGCCTCGCTGCCTTTGGCCGCCAgcg > 6:130968/1‑149 (MQ=255) tGGAAGTTACGATTGAATGGGGCTGCTGCGCAGCCCCAAAAAATCTAGCGCAACACCGCCATTCGTCCGACCAACGGTAACGTGGACAGCAGCTTGAACAACGGCGCt > 7:202104/1‑108 (MQ=255) tGGAAGTTACGATTGAATGGGGCTGCTGCGCAGCCCCAAAAAATCTAGCGCAACACCGCCATTCGTCCGACCAACGGTAACGTGGACAGCAGCTTGAACAACGGCGCt < 8:202104/108‑1 (MQ=255) gTTACGATTGAATGGGGCTGCTGCGCAGCCCCAAAAAATCTAGCGCAACACCGCCATTCGTCCGACCAACGGTAACGTGGACAGCAGCTTGAACAACGGCGCTGTCCAGCGCATCAACGCCTCGCTGCCTTTGGCCGCCAGCGGCgtg > 3:59234/1‑148 (MQ=255) cGATTGAATGGGGCTGCTGCGCAGCCCCAAAAAATCTAGCGCAACACCGCCATTCGTCCGACCAACGGTAACGTGGACAGCAGCTTGAACAACGGCGCTGTCCAGCGCATCAACGCCTCGCTGCCTTTGGCCGCCAGCGGCGTgtagta < 5:167076/149‑1 (MQ=255) cGATTGAATGGGGCTGCTGCGCAGCCCCAAAAAATCTAGCGCAACACCGCCATTCGTCCGACCAACGGTAACGTGGACAGCAGCTTGAACAACGGCGCTGTCCAGCGCATCAACGCCTCGCTGCCTTTGGCCGCCAGCGGCGTgtagt < 6:169118/148‑1 (MQ=255) aTTGAATGGGGCTGCTGCGCAGCCCCAAAAAATCTAGCGCAACACCGCCATTCGTCCGACCAACGGTAACGTGGACAGCAGCTTGAACAACGGCGCTGTCCAGCGCATCAACGCCTCGCTGCCTTTGGCCGCCAGCGGCgt > 7:55965/1‑141 (MQ=255) aTTGAATGGGGCTGCTGCGCAGCCCCAAAAAATCTAGCGCAACACCGCCATTCGTCCGACCAACGGTAACGTGGACAGCAGCTTGAACAACGGCGCTGTCCAGCGCATCAACGCCTCGCTGCCTTTGGCCGCCAGCGGCgt < 8:55965/141‑1 (MQ=255) gAATGGGGCTGCTGCGCAGCCCCAAAAAATCTAGCGCAACACCGCCATTCGTCCGACCAACGGTAACGTGGACAGCAGCTTGAACAACGGCGCTGTCCAGCGCATCAACGCCTCGCTGCCTTTGGCCGCCAGCGGCGTGTAGTAGCTc < 6:146870/148‑1 (MQ=255) aTGGGGCTGCTGCGCAGCCCCAAAAAATCTAGCGCAACACCGCCATTCGTCCGACCAACGGTAACGTGGACAGCAGCTTGAACAACGGCGCTGTCCAGCGCATCAACGCCTCGCTGCCTTTGGCCGCCAGCGGCGTGTAGTAGCTccag > 5:298503/1‑149 (MQ=255) gctgctGCGCAGCCCCAAAAAATCTAGCGCAACACCGCCATTCGTCCGACCAACGGTAACGTGGACAGCAGCTTGAACAACGGCGCTGTCCAGCGCATCAACGCCTCGCTGCCTTTGGCCGCCAGCGGCGTGTAGTAGCTccagcccag < 2:159279/149‑1 (MQ=255) tgcGCAGCCCCAAAAAATCTAGCGCAACACCGCCATTCGTCCGACCAACGGTAACGTGGACAGCAGCTTGAACAACGGCGCTGTCCAGCGCATCAACGCCTCGCTGCCTTTGGCCGCCAGCGGCGTGTAGTAGCTCCAGCCCAgcgc > 1:300328/1‑147 (MQ=255) cgcAGCCCCAAAAAATCTAGCGCAACACCGCCATTCGTCCGACCAACGGTAACGTGGACAGCAGCTTGAACAACGGCGCTGTCCAGCGCATCAACGCCTCGCTGCCTTTGGCCGCCAGCGGCGTGTAGTAGCTCCAGCCCAGCGCCa < 2:159417/147‑1 (MQ=255) | CGAAGAAAGTGTGCGTCGCCGTGAACAGGCGGCTGAAATGCGTAAGGCCGGGGCCATGGGCGTGACCACCGATGGCCGGCCGACCAAGAAGCAGCGGCGGCAGATTCACCAGCTGCATGGAAGTTACGATTGAATGGGGCTGCTGCGCAGCCCCAAAAAATCTAGCGCAACACCGCCATTCGTCCGACCAACGGTAACGTGGACAGCAGCTTGAACAACGGCGCTGTCCAGCGCATCAACGCCTCGCTGCCTTTGGCCGCCAGCGGCGTGTAGTAGCTCCAGCCCAGCGCCA > NC_002947/305010‑305301 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |