Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	3,989,011	A→C	7.9%	H510P (CAC→CCC)	oplA →	5‑oxoprolinase A

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	3,989,011	0	A	C	7.9%	194.5 / 7.5	63	H510P (CAC→CCC)	oplA	5‑oxoprolinase A
Reads supporting (aligned to +/- strand):  ref base A (29/29);  new base C (2/3);  total (31/32)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01

CACGTGGTGCGCCTGGCCGAAGAAATTGGCATGCACACCGTGCTGATCCCCAAGGTGGCCTCGGGCTTGTGTGCGTTCGGCCAGATCCTGTCCGACGTGCGCTA‑CGACCAGCTCACCAGCCTGTCGATGCGCCTGGACGCCGGGCACGTCGACCTGGCGCAACTCAACCAGGCCCTCGCCGAACTACGTCAGCAAGGCCTTGCCAACCTGCGCGAAGACGGCTTTGGCGACCAGGCCAGCAGTTGCCACTACACCCTGGAGATGCGCTACCTGGGGCAGATTCATGAGTGCAGT  >  NC_002947/3988866‑3989159                                                                                                                                                   |                                                                                                                                                     cACGTGGTGCGCCTGGCCGAAGAAATTGGCATGCACACCGTGCTGATCCCCAAGGTGGCCTCGGGCTTGTGTGCGTTCGGCCAGATCCTGTCCGACGTGCGCTA‑CGACCAGCTCACCAGCCTGTCGATGCGCCTGGACGCCGGGCACg                                                                                                                                                    <  5:178320/148‑1 (MQ=255)     tggtgCGCCTGGCCGAAGAAATTGGCATGCACACCGTGCTGATCCCCAAGGTGGCCTCGGGCTTGTGTGCGTTCGGCCAGATCCTGTCCGACGTGCGCTA‑CGACCAGCTCACCAGCCTGTCGATGCGCCTGGACGCCGGGCACGTCGAc                                                                                                                                               <  4:171420/149‑1 (MQ=255)     tggtgCGCCTGGCCGAAGAAATTGGCATGCACACCGTGCTGATCCCCAAGGTGGCCTCGGGCTTGTGTGCGTTCGGCCAGATCCTGTCCGACGTGCGCTA‑CGACCAGCTCACCAGCCTGTCGATGCGCCTGGACGCCGGGCACGTCGAc                                                                                                                                               >  2:23827/1‑149 (MQ=255)          cgcCTGGCCGAAGAAATTGGCATGCACACCGTGCTGATCCCCAAGGTGGCCTCGGGCTTGTGTGCGTTCGGCCAGATCCTGTCCGACGTGCGCTACCTACCAGCTCACCAGCCTGTCGATGCGCCTGGACGCCGGGCACGTCGACCTgg                                                                                                                                           <  1:84771/149‑1 (MQ=255)                gCCGAAGAAATTGGCATGCACACCGTGCTGATCCCCAAGGTGGCCTCGGGCTTGTGTGCGTTCGGCCAGATCCTGTCCGACGTGCGCTA‑CGACCAGCTCACCAGCCTGTCGATGCGCCAGGTCGACGTGCCCGGCGTCCAGGCGCat                                                                                                                                      <  5:222669/147‑2 (MQ=38)                gCCGAAGAAATTGGCATGCACACCGTGCTGATCCCCAAGGTGGCCTCGGGCTTGTGTGCGTTCGGCCAGATCCTGTCCGACGTGCGCTA‑CGACCAGCTCACCAGCCTGTCGATGCGCCAGGTCGACGTGCCCGGCGTCCAGGCGCat                                                                                                                                      <  5:121480/147‑2 (MQ=38)                 ccGAAGAAATTGGCATGCACACCGTGCTGATCCCCAAGGTGGCCTCGGGCTTGTGTGCGTTCGGCCAGATCCTGTCCGACGTGCGCTA‑CGACCAGCTCACCAGCCTGTCGATGCGCCTGGACGCCGGGCACGTCGACCTGGCGCAACt                                                                                                                                    >  7:143665/1‑148 (MQ=255)                   gaagaaATTGGCATGCACACCGTGCTGATCCCCAAGGTGGCCTCGGGCTTGTGTGCGTTCGGCCAGATCCTGTCCTCCGTGCGCTA‑CGACCAGCTCACCAGCCTGTCGATGCGCATGGACGCCGGGCACGTCGCCCTGGCGCACCTCaa                                                                                                                                 >  2:233932/1‑149 (MQ=255)                   gaagaaATTGGCATGCACACCGTGCTGATCCCCAAGGTGGCCTCGGGCTTGTGTGCGTTCGGCCAGATCCTGTCCGACGTGCGCTA‑CGACCAGCTCACCAGCCTGTCGATGCGCCTGGACGCCGGGCACGTCGACCTGGCGCAACTCaa                                                                                                                                 >  4:81414/1‑149 (MQ=255)                   gaagaaATTGGCATGCACACCGTGCTGATCCCCAAGGTGGCCTCGGGCTTGTGTGCGTTCGGCCAGATCCTGTCCGACGTGCGCTA‑CGACCAGCTCACCAGCCTGTCGATGCGCCTGGACGCCGGGCACGTCGACCTGGCACAACTCaa                                                                                                                                 >  5:139440/1‑149 (MQ=255)                        aaTTGGCATGCACACCGTGCTGATCCCCAAGGTGGCCTCGGGCTTGTGTGCGTTCGGCCAGATCCTGTCCGACGTGCGCTA‑CGACCAGCTCACCAGCCTGTCGATGCGCCTGGACGCCGGGCACGTCGACCTGGCGCAACTCAACCAgg                                                                                                                            <  1:319/149‑1 (MQ=255)                         aTTGGCATGCACACCGTGCTGATCCCCAAGGTGGCCTCGGGCTTGTGTGCGTTCGGCCAGATCCTGTCCGACGTGCGCTA‑CGACCAGCTCACCAGCCTGTCGATGCGCCTGGACGCCGGGCACGTCGACCTGGCGCAACTCAACCAGGc                                                                                                                           <  4:279567/149‑1 (MQ=255)                                    cacCGTGCTGATCCCCAAGGTGGCCTCGGGCTTGTGTGCGTTCGGCCAGATCCTGTCCGACGTGCGCTA‑CGACCAGCTCACCAGCCTGTCGATGCGCCTGGACGCCGGGCACGTCGACCTGGCGCAACTCAACCAGGCCCTCGCCGaa                                                                                                                 >  6:102450/1‑148 (MQ=255)                                    cacCGTGCTGATCCCCAAGGTGGCCTCGGGCTTGTGTGCGTTCGGCCAGATCCTGTCCGACGTGCGCTA‑CGACCAGCTCACCAGCCTGTCGATGCGCCTGGACGCCGGGCACGTCGACCTGGCGCAACTCAACCAGGCCCTCGCCGAAc                                                                                                                <  8:143665/149‑1 (MQ=255)                                       cGTGCTGATCCCCAAGGTGGCCTCGGGCTTGTGTGCATTCGGCCAGATCCTGTCCGACGTGCGCTA‑CGACCAGCTCACCAGCCTGTCGATGCGCCTGGACGCCGGGCACGTCGACCTGGCGCAACTCAACCAGGCCCTCGCCGAACTAc                                                                                                             <  5:7142/149‑1 (MQ=255)                                           cTGATCCCCAAGGTGGCCTCGGGCTTGTGTGCGTTCGGCCAGATCCTGTCCGACGTGCGCTA‑CGACCAGCTCACCAGCCTGTCGATGCGCCTGGACGCCGGGCACGTCGACCTGGCGCAACTCAACCAGGCCCTCGCCGAACTACGTc                                                                                                          <  1:23827/148‑1 (MQ=255)                                              aTCCCCAAGGTGGCCTCGGGCTTGTGTGCGTTCGGCCAGATCCTGTCCGACGTGCGCTA‑CGACCAGCTCACCAGCCTGTCGATGCGCCTGGACGCCGGGCACGTCGACCTGGCGCAACTCAACCAGGCCCTCGCCGAACTACGTCAGc                                                                                                       <  6:143230/148‑1 (MQ=255)                                               tCCCCAAGGTGGCCTCGGGCTTGTGTGCGTTCGGCCAGATCCTGTCCGACGTGCGCTA‑CGACCAGCTCACCAGCCTGTCGATGCGCCTGGACGCCGGGCACGTCGACCTGGCGCAACTCAACCAGGCCCTCGCCGAACTACGTCAGCaa                                                                                                     <  3:81414/149‑1 (MQ=255)                                                ccccAAGGTGGCCTCGGGCTTGTGTGCGTTCGGCCAGATCCTGTCCGACGTGCGCTA‑CGACCAGCTCACCAGCCTGTCGATGCGCCAGGTCGACGTGCCCGGCGTCCAGgc                                                                                                                                          >  7:286200/1‑111 (MQ=37)                                                ccccAAGGTGGCCTCGGGCTTGTGTGCGTTCGGCCAGATCCTGTCCGACGTGCGCTA‑CGACCAGCTCACCAGCCTGTCGATGCGCCAGGTCGACGTGCCCGGCGTCCAGgc                                                                                                                                          <  8:286200/111‑1 (MQ=37)                                                 cccAAGGTGGCCTCGGGCTTGTGTGCGTTCGGCCAGATCCTGTCCGACGTGCGCTA‑CGACCAGCTCACCAGCCTGTCGATGCGCCTGGACGCCGGGCACGTCGACCTGGCGCAACTCAACCAGGCCCTCGCCGAACTACGTCAGCAAgg                                                                                                   >  8:188450/1‑149 (MQ=255)                                                 cccAAGGTGGCCTCGGGCTTGTGTGCGTTCGGCCAGATCCTGTCCGACGTGCGCTA‑CGACCAGCTCACCAGCCTGTCGATGCGCCTGGACGCCGGGCACGTCGACCTGGCGCAACTCAACCAGGCCCTCGCCGAACTACGTCAGCAAg                                                                                                    <  3:64888/148‑1 (MQ=255)                                                  ccAAGGTGGCCTCGGGCTTGTGTGCGTTCGGCCAGATCCTGTCCGACGTGCGCTA‑CGACCAGCTCACCAGCCTGTCGATGCGCCTGGACGCCGGGCACGTCGACCTGGCGCAACTCAACCAGGCCCTCGCCGAACTACGTCAGCAAGGc                                                                                                  >  6:94143/1‑149 (MQ=255)                                                          gCCTCGGGCTTGTGCGCGTTCGGCCAGATCCTGTCCGACGTGCGCTA‑CGACCAGCTCACCAGCCTGTCGATGCGCCTGGACGCCGGGCACGTCGACCTGGCGCAACTCAACCAGGCCCTCGCCGAACTACGTCAGCAAAGCCTTGCCaa                                                                                          >  1:85783/1‑149 (MQ=255)                                                             tCGGGCTTGTGTGCGTTCGGCCAGATCCTGTCCGACGTGCGCTA‑CGACCAGCTCACCAGCCTGTCGATGCGCCTGGACGCCGGGCACGTCGACCTGGCGCAACTCAACCAGGCCCTCGCCGAACTACGTCAGCAAGGCCtt                                                                                               <  3:221771/141‑1 (MQ=255)                                                             tCGGGCTTGTGTGCGTTCGGCCAGATCCTGTCCGACGTGCGCTA‑CGACCAGCTCACCAGCCTGTCGATGCGCCTGGACGCCGGGCACGTCGACCTGGCGCAACTCAACCAGGCCCTCGCCGAACTACGTCAGCAAGGCCtt                                                                                               >  4:221771/1‑141 (MQ=255)                                                                 gCTTGTGTGCGTTAGGCCAGATCCTGTCCGACGTGCGCTA‑CGACCAGCTCACCAGCCTGTCGATGCGCCTGGACGCCGGGCACGTCGACCTGGCGCAACTCAACCAGGCCCTCTCCGAACTACGTCAGCAAGGCCTTGCCAACCTgcgc                                                                                   <  7:189516/149‑1 (MQ=255)                                                                   ttGTGTGCGTTCGGCCAGATCCTGTCCGACGAGCGCTA‑CGACCAGCTCACCAGCCTGTCCATCCGCCTGGACGCCGGGCACTACGACCTGGCGCAACTCAACCAGGCCCTCGCCGAACTACCTCAGCAAGGCCTTGCCAACCTccgcc                                                                                  >  2:79556/1‑147 (MQ=255)                                                                        tgCGTTCGGCCAGATCCTGTCCGACGTGCGCTA‑CGACCAGCTCACCAGCCTGTCGATGCGCCTGGACGCCGGGCACGTCGACCTGGCGCAACTCAACCAGGCCCTCGCCGAACTACGTCAGCAAGGCCTTg                                                                                              >  7:79565/1‑131 (MQ=255)                                                                        tgCGTTCGGCCAGATCCTGTCCGACGTGCGCTA‑CGACCAGCTCACCAGCCTGTCGATGCGCCTGGACGCCGGGCACGTCGACCTGGCGCAACTCAACCAGGCCCTCGCCGAACTACGTCAGCAAGGCCTTg                                                                                              <  8:79565/131‑1 (MQ=255)                                                                          cGTTCGGCCAGATCCTGTCCGACGTGCGCTA‑CGACCAGCTCACCAGCCTGTCGATGCGCCTGGACGCCGGGCACGTCGACCTGGCGCAACTCAACCAGGCCCTCGCCGAACTACGTCAGCAAGGCCTTGCCAACCTGCGCGAAGACGGc                                                                          >  1:270341/1‑149 (MQ=255)                                                                           gTTCGGCCAGATCCTGTCCGACGTGCGCTA‑CGACCAGCTCACCAGCCTGTCGATGCGCCTGGACGCCGGGCACGTCGACCTGGCGCAACTCAACCAGGCCCTCGCCGAACTACGTCAGCAAGGCCTTGCCAACCTGCGCGAAGACGGCt                                                                         >  8:37334/1‑149 (MQ=255)                                                                                      tCCTGTCCGACGTGCGCTA‑CGACCAGCTCACCAGCCTGTCGATGCGCCTGGACGCCGGGCACGTCGACCTGGCGCAACTCAACCAGGCCCTCGCCGAACTACGTCAGCAAGGCCTTGCCAACCTGCGCGAAGACGGCTTTGGCGACCAg                                                              <  7:37334/149‑1 (MQ=255)                                                                                      tCCTGTCCGACGTGCGCTA‑CGACCAGCTCACCAGCCTGTCGATGCGCCTGGACGCCGGGCACGTCGACCTGGCGCAACTCAACCAGGCCCTCGCCGAACTACGTCAGCAAGGCCTTGCCAACCTGCGCGAAGACGGCTTTGGCGACCAg                                                              <  5:260114/149‑1 (MQ=255)                                                                                       ccTGTCCGACGTGCGCTA‑CGACCAGCTCACCAGCCTGTCGATGCGCCTGGACGCCGGGCACGTCGACCTGGCGCAACTCAACCAGGCCCTCGCCGAACTACGTCAGCAAGGCCTTGCCAACCTGCGCGAAGACGGCTTTGGCGACCAgg                                                             >  6:109090/1‑149 (MQ=255)                                                                                        cTGTCCGACGTGCGCTA‑CGACCAGCTCACCAGCCTGTCGATGCGCCTGGACGCCGGGCACGTCGACCTGGCGCAACTCAACCAGGCCCTCGCCGAACTACGTCAGCAAGGCCTTGCCAACCTGCGCGAAGACGGCTTTGGCGACCAGGc                                                            >  1:104974/1‑149 (MQ=255)                                                                                             cGACGTGCGCTA‑CGACCAGCTCACCAGCCTGTCGATGCGCCTGGACGCCGGGCACGTCGACCTGGCGCAACTCAACCAGGCCCTCGCCGAACTACGTCAGCAAGGCCTTGCCAACCTGCGCGAAGACGGCTTTGGCGACCAGGCcag                                                         >  5:153520/1‑147 (MQ=255)                                                                                             cGACGTGCGCTA‑CGACCAGCTCACCAGCCTGTCGATGCGCCTGGACGCCGGGCACGTCGACCTGGCGCAACTCAACCAGGCCCTCGCCGAACTACGTCAGCAAGGCCTTGCCAACCTGCGCGAAGACGGCTTTGGCGACCAGGCcag                                                         >  5:162554/1‑147 (MQ=255)                                                                                                     gcTA‑CGACCAGCTCACCAGCCTGTCGATGCGCCTGGACGCCGGGCACGTCGACCTGGCGCAACTCAACCAGGCCCTCGCCGAACTACGTCAGCAAGGCCTTGCCAACCTGCGCGAAGACGGCTTTGGCGACCAGGCCAGCAGTTGCCAc                                               <  2:270341/149‑1 (MQ=255)                                                                                                       tA‑CGACCAGCTCACCAGCCTGTCGATGCGCCTGGACGCCGGGCACGTCGACCTGGCGCAACTCAACCAGGCCCTCGCCGAACTACGTCAGCAAGGCCTTGCCAACCTGCGCGAAGACGGCTTTGGCGACCAGGCCAGCAGTTGCCACTa                                             >  7:219168/1‑149 (MQ=255)                                                                                                             ccAGCTCACCAGCCTGTCGATGCGCCTGGACGCCGGGCACGTCGACCTGGCGCAACTCAACCAGGCCCTCGCCGAACTAAGTCAGCAAGGCCTTGCCAACCTGCGCGAAGACGGCTTTGGCGACCAGGCCAGCAGTTGCCACTACAcc                                         >  8:128388/1‑148 (MQ=255)                                                                                                               aGCTCACCAGCCTGTCGATGCGCCTGGACGCCGGGCACGTCGACCTGGCGCAACTCAACCAGGCCCTCGCCGAACTACGTCAGCAAGGCCTTGCCAACCTGCGCGAAGACGGCTTTGGCGACCAGGCCAGCAGTTGCCACTACACCCTg                                      <  5:94143/149‑1 (MQ=255)                                                                                                                 cTCACCAGCCTGTCGATGCGCCTGGACGCCGGGCACGTCGACCTGGCGCAACTCAACCAGGCCCTCGCCGAACTACGTCAGCAAGGCCTTGCCAACCTGCGCGAAGACGGCTTTGGCGACCAGGCCAGCAGTTGCCACTACACCCTGga                                    <  5:109090/149‑1 (MQ=255)                                                                                                                  tCACCAGCCTGTCGATGCGCCTGGACGCCGGGCACGTCGACCTGGCGCAACTCAACCAGGCCCTCGCCGAACTACGTCAGCAAGGCCTTGCCAACCTGCGCGAAGACGGCTTTGGCGACCAGGCCAGCAGTTGCCACTACACCCTg                                      >  5:187300/1‑146 (MQ=255)                                                                                                                    aCCAGCCTGTCGATGCGCCTGGACGCCGGGCACGTCGACCTGGCGCAACTCAACCAGGCCCTCGCCGAACTACGTCAGCAAGGCCTTGCCAACCTGCGCGAAGACGGCTTTGGCGACCAGGCCAGCAGTTGCCACTACACCCTGGAGAt                                 >  2:55412/1‑149 (MQ=255)                                                                                                                      cAGCCTGTCGATGCGCCTGGACGCCGGGCACGTCGACCTGGCGCAACTCAACCAGGCCCTCGCCGAACTACGTCAGCAAGGCCTTGCCAACCTGCGCGaa                                                                                <  5:253101/100‑1 (MQ=255)                                                                                                                      cAGCCTGTCGATGCGCCTGGACGCCGGGCACGTCGACCTGGCGCAACTCAACCAGGCCCTCGCCGAACTACGTCAGCAAGGCCTTGCCAACCTGCGCGaa                                                                                >  6:253101/1‑100 (MQ=255)                                                                                                                        gCCTGTCGATGCGCCTGGACGCCGGGCACGTCGACCTGGCGCAACTCAACCAGGCCCTCGCCGAACTACGTCAGCAAGGCCTTGCCAACCTGCGCGAAGACGGCTTTGGCGACCAGGCCAGCAGTTGCCACTACACCCTGGAGATgcgc                             >  2:315013/1‑149 (MQ=255)                                                                                                                         ccTGTCGATGCGCCTGGACGCCGGGCACGTCGACCTGGCGCAACTCAACCAGGCCCTCGCCGAACTACGTCAGCAAAGCCTTGCCAACCTGCGCGAAGACGGCTTTGGCGACCAGGCCAGCAGTTGCCACTACACCCTGGAGATGCGCt                            <  2:85783/149‑1 (MQ=255)                                                                                                                             tCGATGCGCCTGGACGCCGGGCACGTCGACCTGGCGCAACTCAACCAGGCCCTCGCCGAACTACGTCAGCAAGGCCTTGCCAACCTGCGCGAAGACGGCTTTGGCGACCAGGCCAGCAGTTGCCACTACACCCTGGAGATGCGCTACCt                        <  1:49085/149‑1 (MQ=255)                                                                                                                               gATGCGCCTGGACGCCGGGCACGTCGACCTGGCGCAACTCAACCAGGCCCTCGCCGAACTACGTCAGCAAGGCCTTGCCAACCTGCGCGATGACGGCTTTGGCGACCAGGCCAGCAGTTGCCACTa                                             <  1:51191/126‑1 (MQ=255)                                                                                                                               gATGCGCCTGGACGCCGGGCACGTCGACCTGGCGCAACTCAACCAGGCCCTCGCCGAACTACGTCAGCAAGGCCTTGCCAACCTGCGCGAAGACGGCTTTGGCGACCAGGCCAGCAGTTGCCACTa                                             >  2:51191/1‑126 (MQ=255)                                                                                                                                aTGCGCCTGGACGCCGGGCACGTCGACCTGGCGCAACTCAACCAGGCCCTCGCCGAACTACGTCAGCAAGGCCTTGCCAACCTGCGCGAAGACGGCTTTGGCGACCAGGCCAGCAGTTGCCACTACACCCTGGAGATGCGCTACCTggg                     <  6:162554/149‑1 (MQ=255)                                                                                                                                aTGCGCCTGGACGCCGGGCACGTCGACCTGGCGCAACTCAACCAGGCCCTCGCCGAACTACGTCAGCAAGGCCTTGCCAACCTGCGCGAAGACGGCTTTGGCGACCAGGCCAGCAGTTGCCACTACACCCTGGAGATGCGCTACCTggg                     >  4:292329/1‑149 (MQ=255)                                                                                                                                  gcgcCAGGTCGACGTGCCCGGCGTCCAGGCGCAACTCAACCAGGCCCTCGCCGAACTACGTCAGCAAGGCCTTGCCAACCTGCGCGAAGACGGCTTTGGCGACCAGGCCAGCAGTTGCCACTACACCCTGGAGATGCGCTACCTgggg                    >  5:162412/1‑148 (MQ=255)                                                                                                                                   cgcCTGGACGCCGGGCACGTCGACCTGGCGCAACTCAACCAGGCCCTCGCCGAACTACGTCAGCAAGGCCTTGCCAACCTGCGCGAAGACGGCTTTGGCGACCAGGCCAGCAGTTGCCACTACACCCTGGAGATGCGCTACCTGGGGc                   <  1:233932/148‑1 (MQ=255)                                                                                                                                    gcCTGGACGCCGGGCACGTCGACCTGGCGCAACTCAACCAGGCCCTCGCCGAACTACGTCAGCAAGGCCTTGCCAACCTGCGCGAAGACGGCTTTGGCGACCAGGCCAGCAGTTGCCACTACACCCTGGAGATGCGCTACCTgggg                    >  2:227057/1‑146 (MQ=255)                                                                                                                                     ccTGGACGCCGGGCACGTCGACCTGGCGCAACTCAACCAGGCCCTCGCCGAACTACGTCAGCAAGGCCTTGCCAACCTGCGCGAAGACGGCTTTGGCGACCAGGCCAGCAGTTGCCACTACACCCTGGAGATGCGCTACCTGGGGCAGa                <  2:42621/149‑1 (MQ=255)                                                                                                                                          aCGCCGGGCACGTCGACCTGGCGCAACTCAACCAGGCCCTCGCCGAACTACGTCAGCAAGGCCTTGCCAACCTGCGCGAAGACGGCTTTGGCGACCAGGCCAGCAGTTGCCACTACACCCTGGAGATGCGCTACCTGGGGCAGATTCAt           <  6:153520/149‑1 (MQ=255)                                                                                                                                           cGCCGGGCACGTCTACCTGGCGCAACTCAACCAGGCACTCGCCGAACTACGTCAGCAAGGCCTTGCCAACCTGCGCGAAGACGGCTTTGGCGACCAGCCCAAAAGTTGCCACTACACACTGGAGATGCGCTACCTGGGGCAGATTCAt           >  8:249860/1‑148 (MQ=255)                                                                                                                                            gCCGGGCACGTCGACCTGGCACAACTCAACCAGGCCCTCGCCGAACTACGTCAGCAAGGCCTTGCCAACCTGCGCGAAGACGGCTTTGGCGACCAGGCCAGCAGTTGCCACTACACCCTGGAGATGCGCTACCTGGGGCAGATTCATGa         <  6:139440/149‑1 (MQ=255)                                                                                                                                                 gCACGTCGACCTGGCGCAACTCAACCAGGCCCTCGCCGAACTACGTCAGCAAGGCCTTGCCAACCTGCGCGAAGACGGCTTTGGCGACCAGGCCAGCAGTTGCCACTACACCCTGGAGATGCGCTACCTGGGGCAGATTCATGAGTg      >  3:19433/1‑147 (MQ=255)                                                                                                                                                 gCACGTCGACCTGGCGCAACTCAACCAGGCCCTCGCCGAACTACGTCAGCAAGGCCTTGCCAACCTGCGCGAAGACGGCTTTGGCGACCAGGCCAGCAGTTGCCACTACACCCTGGAGATGCGCTACCTGGGGCAGATTCATGAGTGCa    <  1:55412/149‑1 (MQ=255)                                                                                                                                                   aCGTCGACCTGGCGCAACTCAACCAGGCCCTCGCCGAACTACGTCAGCAAGGCCTTGCCAACCTGCGCGAAGACGGCTTTGGCGACCAGGCCAGCAGTTGCCACTACACCCTGGAGATGCGCTACCTGGGGCAGATTCATGAGTGCAgt  <  7:188450/149‑1 (MQ=255)                                                                                                                                                   |                                                                                                                                                     CACGTGGTGCGCCTGGCCGAAGAAATTGGCATGCACACCGTGCTGATCCCCAAGGTGGCCTCGGGCTTGTGTGCGTTCGGCCAGATCCTGTCCGACGTGCGCTA‑CGACCAGCTCACCAGCCTGTCGATGCGCCTGGACGCCGGGCACGTCGACCTGGCGCAACTCAACCAGGCCCTCGCCGAACTACGTCAGCAAGGCCTTGCCAACCTGCGCGAAGACGGCTTTGGCGACCAGGCCAGCAGTTGCCACTACACCCTGGAGATGCGCTACCTGGGGCAGATTCATGAGTGCAGT  >  NC_002947/3988866‑3989159

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑