| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 4,079,170 | T→A | 9.4% | E9D (GAA→GAT) ‡ | sdaC ← | serine:H+ symport permease |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 4,079,170 | 0 | T | A | 9.4% | 142.3 / 5.8 | 53 | E9D (GAA→GAT) ‡ | sdaC | serine:H+ symport permease |
| Reads supporting (aligned to +/- strand): ref base T (23/25); new base A (3/2); total (26/27) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.69e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.63e-01 | |||||||||||
GCAGCGGCCAGAAGCCGCCAAGGCCGGCGTTGATCGGCAGGAACAGTGTCCCGGCGCCGATGGCTGTGCCGAACAGGCCCAGCATCCAGGTGGTGTCGTGACGCGCCCAGGTGCCGAGGGCGGC‑GGGGGTCGATTCTGCAAAGCGTTGTTCAACGCTTGGGGCCTGCTCATTCATTCCGGGTGAAGCTCCACTCGCAAGACTGCAACAGGCTGAGAATGGCGAAATAGACGTTTCGCCCAACTCAACCGGAAAAGAGGGGCGCGATTGTGCACGGAAAGGTTGCACTTGCGAA > NC_002947/4079022‑4079314 | gcagcGGCCAGAAGCCGCCAAGGCCGGCGTTGATCGGCAGGAACAGTGTCCCGGCGCCGATGGCTGTGCCGAACAGGCCCAGCATCCAGGTGGTGTCGTGACGCGCCCAGGTGCCGAGGGCGGC‑GGGGGTCGATTCTGCAAAGCgttgt > 6:65555/1‑149 (MQ=255) cagcGGCCAGAAGCCGCCAAGGCCGGCGTTGATCGGCAGGAACAGTGTCCCGGCGCCGATGGCTGTGCCGAACAGGCCCAGCATCCAGGTGGTGTCGTGACGCGCCCAGGTGCCGAGGGCGGC‑GGGGGTCGATTCTGCAAAGCgttgtt > 3:96643/1‑149 (MQ=255) cagcGGCCAGAAGCCGCCAAGGCCGGCGTTGATCGGCAGGAACAGTGTCCCGGCGCCGATGGCTGTGCCGAACAGGCCCAGCATCCAGGTGGTGTCGTGACGCGCCCAGGTGCCGAGGGCGGC‑GGGGGTCGATTCTGCAAAGCgttgtt < 7:202589/149‑1 (MQ=255) gcGGCCAGAAGCCGCCAAGGCCGGCGTTGATCGGCAGGAACAGTGTCCCGGCGCCGATGGCTGTGCCGAACAGGCCCAGCATCCAGGTGGTGTCGTGACGCGCCCAGGTGCCGAGGGCGGA‑GGGGGTCGATTCTGCAAAGCGTTGTTCa > 5:206167/1‑149 (MQ=255) aaGCCGCCAAGGCCGGCGTTGATCGGCAGGAACAGTGTCCCGGCGCCGATGGCTGTGCCGAACAGGCCCAGCATCCAGGTGGTGTCGTGACGCGCCCAGGTGCCGAGGGCGGC‑GGGGGTCGATTCTGCAAAGCGTTGTTCAACGCTTgg < 5:65555/149‑1 (MQ=255) aaGCCGCCAAGGCCGGCGTTGATCGGCAGGAACAGTGTCCCGGCGCCGATGGCTGTGCCGAACAGGCCCAGCATCCAGGTGGTGTCGTGACGCGCCCAGGTGCCGAGGGCGGC‑GGGGGTCGATTCTGCAAAGCGTTGTTCAACGCTTgg > 2:196974/1‑149 (MQ=255) aGCCGCCAAGGCCGGCGTTGATCGGCAGGAACAGTGTCCCGGCGCCGATGGCTGTGCCGAACAGGCCCAGCATCCAGGTGGTGTCGTGACGCGCCCAGGTGCCGAGGGCGGC‑GGGGGTCGATTCTGCAAAGCGTTGTTCAACGCTTggg > 4:323235/1‑149 (MQ=255) ccgccAAGGCCGGCGTTGATCGGCAGGAACAGTGTCCCGGCGCCGATGGCTGTGCCGAACAGGCCCAGCATCCAGGTGGTGTCGTGACGCGCCCAGGTGCCGAGGGCGGC‑GGGGGTCGATTCTGCAAAGCGTTGTTCAACGCTTGGGGc < 1:19625/149‑1 (MQ=255) aaGGCCGGCGTTGATCGGCAGGAACAGTGTCCCGGCGCCGATGGCTGTGCCGAACAGGCCCAGCATCCAGGGGGTGTCGTGACGCGCCCAGGTGCCGAGGGCGGC‑GGGGGGCGATTCTGCAAAGCGTTGTTCAACGCTTGGGGCCTGCt < 7:252948/149‑1 (MQ=255) tCGGCAGGAACAGTGTCCCGGCGCCGATGGCTGTGCCGAACAGGCCCAGCATCCAGGTGGTGTCGTGACGCGCCCAGGTGCCGAGGGCGGC‑GGGGGTCGATTCTGCAAAGCGTTGTTCAACGCTTGGGGCCt > 6:184863/1‑132 (MQ=255) tCGGCAGGAACAGTGTCCCGGCGCCGATGGCTGTGCCGAACAGGCCCAGCATCCAGGTGGTGTCGTGACGCGCCCAGGTGCCGAGGGCGGC‑GGGGGTCGATTCTGCAAAGCGTTGTTCAACGCTTGGGGCCt < 5:184863/132‑1 (MQ=255) tCGGCAGGAACAGTGTCCCGGCGCCGATGGCTGTGCCGAACAGGCCCAGCATCCAGGTGGTGTCGTGACGCGCCCAGGTGCCGAGGGCGGC‑GGGGGTCGATTCTGCAAAGCGTTGTTCAACGCTTGGGGCCTGCTCATTCATTCCGGGt < 4:14009/149‑1 (MQ=255) cGGCAGGAACAGTGTCCCGGCGCCGATGGCTGTGCCGAACAGGCCCAGCATCCAGGTGGTGTCGTGACGCGCCCAGGTGCCGAGGGCGGC‑GGGGGTCGATTCTGCAAAGCGTTGTTCAACGCTTGGGGCCTGCTCATTCATTCCGGGt < 4:113283/148‑1 (MQ=255) ggCAGGAACAGTGTCCCGGCGCCGATGGCTGTGCCGAACAGGCCCAGCATCCAGGTGGTGTCGTGACGCGCCCAGGTGCCGAGGGCGGC‑GGGGGTCGATTCTGCAAAGCGTTGTTCAACGCTTGGGGCCTGCTCATTCATTCCGGGTg > 5:98229/1‑148 (MQ=255) aGGAACAGTGTCCCGGCGCCGATGGCTGTGCCGAACAGGCCCAGCATCCAGGTGGTGTCGTGACGCGCCCAGGTGCCGAGGGCGGC‑GGGGGTCGATTCTGCAAAGCGTTGTTCAACGCTTGGGGCCTGCTCATTCATTCCGGGTGAAGc > 5:95939/1‑149 (MQ=255) aGTGTCCCGGCGCCGATGGCTGTGCCGAACAGGCCCAGCATCCAGGTGGTGTCGTGACGCGCCCAGGTGCCGAGGGCGGC‑GGGGGTCGATTCTGCAAAGCGTTGTTCAACGCTTGGGGCCTGCTCATTCATTCCGGGTGAAGCTCCACt < 7:239798/149‑1 (MQ=255) tCCCGGCGCCGATGGCTGTGCCGAACAGGCCCAGCATCCAGGTGGTGTCGTGACGCGCCCAGGTGCCGAGGGCGGC‑GGGGGTCGATTCTGCAAAGCGTTGTTCAACGCTTGGGGCCTGCTCATTCATTCCGGGTGAAGCTCCACTCGc > 5:214591/1‑148 (MQ=255) tCCCGGCGCCGATGGCTGTGCCGAACAGGCCCAGCATCCAGGTGGTGTCGTGACGCGCCCAGGTGCCGAGGGCGGC‑GGGGGTCGATTCTGCAAAGCGTTGTTCAACGCTTGGGGCCTGCTCATTCATTCCGGGTGAAGCTCCACTCGc > 6:173198/1‑148 (MQ=255) ccGGCGCCGATGGCTGTGCCGAACAGGCCCAGCATCCAGGTGGTGTCGTGACGCGCCCAGGTGCCGAGGGCGGC‑GGGGGTCGATTCTGCAAAGCGTTGTTCAACGCTTGGGGCCTGCTCATTCATTCCGGGTGAAGCTCCACTCGc > 4:83016/1‑146 (MQ=255) ccGGCGCCGATGGCTGTGCCGAACAGGCCCAGCATCCAGGTGGTGTCGTGACGCGCCCAGGTGCCGAGGGCGGC‑GGGGGTCGATTCTGCAAAGCGTTGTTCAACGCTTGGGGCCTGCTCATTCATTCCGGGTGAAGCTACACTCga > 8:122822/1‑145 (MQ=255) tGGCTGTGCCGAACAGGCCCAGCATCCAGGTGGTGTCGTGACGCGCCCAGGTGCCGAGGGCGGC‑GGGGGTCGATTCTGCAAAGCGTTGTTCAACGCTTGGGGCCTGCTCATTCATTCCGGGTGAAGCTCCACTCGCAAGACTGCAACAg > 2:334499/1‑149 (MQ=255) tGGCTGTGCCGAACAGGCCCAGCATCCAGGTGGTGTCGTGACGCGCCCAGGTGCCGAGGGCGGC‑GGGGGTCGATTCTGCAAAGCGTTGTTCAACGCTTGGGGCCTGCTCATTCATTCCGGGTGAAGCTCCACTCGCAAGACTGCAACAg > 3:51128/1‑149 (MQ=255) gCTGTGCCGAACAGGCCCAGCATCCAGGTGGTGTCGTGACGCGCCCAGGTGCCGAGGGCGGC‑GGGGGTCGATTCTGCAAAGCGTTGTTCAACGCTTGGGGCCTGCTCATTCATTCCGGGTGAAGCTCCACTCGCAAGACTGCAACAGGc > 4:73058/1‑149 (MQ=255) gAACAGGCCCAGCATCCAGGTGGTGTCGTGACGCGCCCAGGTGCCGAGGGCGGC‑GGGGGTCGATTCTGCAAAGCGTTGTTCAACGCTTGGGGCCTGCTCATTCATTCCGGGTGAAGCTCCACTCGCAAGACTGCAACAGGCTGAGAATg < 1:334499/149‑1 (MQ=255) ttcggCACAGCATCCAGGTGGTGTCGTGACGCGCCCAGGTGCCGAGGGCGGC‑GGGGGTCGATTCTGCAAAGCGTTGTTCAACGCTTGGGGCCTGCTCATTCATTCCGGGTGAAGCTCCACTCGCAAGACTGCAACAGGCTGAGAATgg < 8:44025/145‑1 (MQ=255) aCAGGCCCAGCATCCAGGGGGTGTCGTGACGCGCCCAGGTGCCGAGGGCGGC‑GGGGGTCGATTCTGCAAAGCGTTGTTCAACGCTTGGGGCCTGCTCATTCATTCCGGGTGAAGCTCCACTCGCAAGACTGCAACAGGCTGAGAATGGc < 2:311214/149‑1 (MQ=255) cAGGCCCAGCATCCAGGTGGTGTCGTGACGCGCCCAGGTGCCGAGGGCGGC‑GGGGGTCGATTCTGCAAAGCGTTGTTCAACGCTTGGGGCCTGCTCATTCATTCCGGGTGAAGCTCCACTCGCAAGACTGCAACAGGCTGAGAATGGCg < 3:83016/149‑1 (MQ=255) gCCCAGCATCCAGGTGGTGTCGTGACGCGCCCAGGTGCCGAGGGCGGC‑GGGGGTCGATTCTGCAAAGCGTTGTTCAACGCTTGGGGCCTGCTCATTCATTCCGGGTGAAGCTCCACTCGCAAGACTGCAACAGGCTGAGAATGGCGaaa > 8:102257/1‑149 (MQ=255) gCCCAGCATCCAGGTGGTGTCGTGACGCGCCCAGGTGCCGAGGGCGGC‑GGGGGTCGATTCTGCAAAGCGTTGTTCAACGCTTGGGGCCTGCTCATTCATTCCGGGTGAAGCTCCACTCGCAAGACTGCAACAGGCTGAGAATGGCGaaa < 3:323235/149‑1 (MQ=255) ccAGCATCCAGGTGGTGTCGTGACGCGCCCAGGTGCCGAGGGCGGC‑GGGGGTCGATTCTGCAAAGCGTTGAACAACGCttt > 1:37466/1‑80 (MQ=255) ccAGCATCCAGGTGGTGTCGTGACGCGCCCAGGTGCCGAGGGCGGC‑GGGGGTCGATTCTGCAAAGCGTTGAACAACGCttt < 2:37466/81‑2 (MQ=255) gCATCCAGGTGGTGTCGTGACGCGCCCAGGTGCCGTGGGCGTC‑GGGGGTCGATTCTGCAAAGCGTTGTTCAACGCTTGGGGCCTGCTCATTCATTCCTGGTGAAGCTCCACTCGCAAGACTGCAACAGGCTGAGAATGGCGAAATAGAc < 1:255407/149‑1 (MQ=255) cATCCAGGTGGTGTCGTGACGCGCCCAGGTGCCGAGGGCGGC‑GGGGGTCGATTCTGCAAAGCGTTGTTCAACGCTTGGGGCCTGCTCATTCATTCCGGGTGAAGCTCCACTCGCAAGACTGCAACAGGCTGAGAATGGCGAAATAGACg < 2:22278/149‑1 (MQ=255) aTCCAGGTGGTGTCGTGACGCGCCCAGGTGCCGAGGGCGGCGGGGGGTCGATTCTGCAAAGCGTTGTTCAACGCTTGGGGCCTGCTCATTCATTCCGGGTGAAGCTCCACTCGCAAGACTGCAACAGGCTGAGAATGGCGAAATAGACg > 1:70801/1‑149 (MQ=255) aGGTGGTGTCGTGACGCGCCCAGGTGCCGAGGGCGGC‑GGGGGTCGATTCTGCAAAGCGTTGTTCAACGCTTGGGGCCTGCTCATTCATTCCGGGTGAAGCTCCACTCGCAAGACTGCAACAGGCTGAGAATGGCGAAATAGACGTTTCg < 6:214591/149‑1 (MQ=255) aGGTGGTGTCGTGACGCGCCCAGGTGCCGAGGGCGGC‑GGGGGTCGATTCTGCAAAGCGTTGTTCAACGCTTGGGGCCTGCTCATTCATTCCGGGTGAAGCTCCACTCGCAAGACTGCAACAGGCTGAGAATGGCGAAATAGACGTTTCg < 3:329760/149‑1 (MQ=255) gtggtgTCGTGACGCGCCCAGGTGCCGAGGGCGGC‑GGGGGTCGATTCTGCAAAGCGTTGTTCAACGCTTGGGGCCTGCTCATTCATTCCGGGTGAAGCTCCACTCGCAAGACTGCAACAGGCTGAGAATGGCGAAATAGACGTTTCGcc < 4:96643/149‑1 (MQ=255) gtCGTGACGCGCCCAGGTGCCGAGGGCGGC‑GGGGGTCGATTCTGCAAAGCGTTGTTCAACGCTTGGGGCCTGCTCATTCATTCCGGGTGAAGCTCCACTCGCAAGACTGCAACAGGCTGAGAATGGCGAAATAGACGTTTCGCCCAACt > 5:244554/1‑149 (MQ=255) tCGTGACGCGCCCAGGTGCCGAGGGCGGC‑GGGGGTCGATTCTGCAAAGCGTTGTTCAACGCTTGGGGCCTGCTCATTCATTCCGGGTGAAGCTCCACTCGCAAGACTGCAACAGGCTGAGAATGGCGAAATAGACGTTTCggcgaaag > 3:244723/1‑141 (MQ=255) ccAGGTGCCGAGGGCGGC‑GGGGGTCGATTCTGCAAAGCGTTGTTCAACGCTTGGGGCCTGCTCATTCATTCCGGGTGAAGCTCCACTCGCAAGACTGCAACAGGCTGAGAATGGCGAAATAGACGTTTCGCCCAACTCAACCGGaa > 4:71026/1‑146 (MQ=255) ggTGCCGAGGGCGGC‑GGGGGTCGATTCTGCAAAGCGTTGTTCAACGCTTGGGGCCTGCTCATTCATTCCGGGTGAAGCTCCACTCGCAAGACTGCAACAGGCTGAGAATGGCGAAATAGACGTTTCGCCCAACTCAACCGGAAAAGAgg < 6:206167/149‑1 (MQ=255) gTGCCGAGGGCGGC‑GGGGGTCGATTCTGCAAAGCGTTGTTCAACGCTTGGGGCCTGCTCATTCATTCCGGGTGAAGCTCCACTCGCAAGACTGCAACAGGCTGAGAATGGCGAAATAGACGTTTCGCCCAACTCAACCGGAAAAGAggg < 6:95939/149‑1 (MQ=255) ccGAGGGCGGC‑GGGGGTCGATTCTGCAAAGCGTTGTTCAACGCTTGGGGCCTGCTCATTCATTCCGGGTGAAGCTCCACTCGCAAGACTGCAACAGGCTGAGAATGGCGAAATAGACGTTTCGCCCAACTCAACCGGAAAAGAGGGgc < 1:196974/148‑1 (MQ=255) aGGGCGGC‑GGGGGTCGATTCTGCAAAGCGTTGTTCAACGCTTGGGGCCTGCTCATTCATTCCGGGTGAAGCTCCACTCGCAAGACTGCAACAGGCTGAGAATGGCGAAATAGACGTTTCGCCCAACTCAACCGGAAAAGAGGGGCGCGa > 7:283441/1‑149 (MQ=255) ggc‑ggGGGTCGATTCTGCAAAGCGTTGTTCAACGCTTGGGGCCTGCTCATTCATTCCGGGTGAAGCTCCACTCGCAAGACTGCAACAGGCTGAGAATGGCGAAATAGACGTTTCGCCCAACTCAACCGGAAAAGAGGGGCGCGATtgtg > 6:294359/1‑149 (MQ=255) gc‑ggGGGTCGATTCTGCAAAGCGTTGTTCAACGCTTGGGGCCTGCTCATTCATTCCGGGTGAAGCTCCACTCGCAAGACTGCAACAGGCTGAGAATGGCGAAATAGACGTTTCGCCCAACTCAACCGGAAAaga > 6:144571/1‑134 (MQ=255) gc‑ggGGGTCGATTCTGCAAAGCGTTGTTCAACGCTTGGGGCCTGCTCATTCATTCCGGGTGAAGCTCCACTCGCAAGACTGCAACAGGCTGAGAATGGCGAAATAGACGTTTCGCCCAACTCAACCGGAAAaga < 5:144571/134‑1 (MQ=255) gc‑ggGGGTCGATTCTGCAAAGCGTTGTTCAACGCTTGGGGCCTGCTCATTCATTCCGGGTGAAGCTCCACTCGCAAGACTGCAACAGGCTGAGAATGGCGAAATAGACGTTTCGCCCAACTCAACCGGAAAAGAGGGGCGCGATTGTGc < 3:71026/149‑1 (MQ=255) c‑ggGGGTCGATTCTGCAAAGCGTTGTTCAACGCTTGGGGCCTGCTCATTCATTCCGGGTGAAGCTCCACTCGCAAGACTGCAACAGGCTGAGAATGGCGAAATAGACGTTTCGCCCAACTCAACCGGAAAAGAGGGGCGCGATTGTGCa < 4:51128/149‑1 (MQ=255) ttCTGCAAAGCGTTGTTCAACGCTTGGGGCCTGCTCATTCATTCCGGGTGAAGCTCCACTCGCAAGACTGCAACAGGCTGAGAATGGCGAAATAGACGTTTCGCCCAACTCAACCGGAAAAGAGGGGCGCGATTGTGCACGGAAAGGtt < 7:102257/149‑1 (MQ=255) aggccccaaGCGTTGAACAACGCTTTGGGCCTGCTCATTCATTCCGGGTGAAGCTCCACTCGCAAGACTGCAACAGGCTGAGAATGGCGAAATAGACGTTTCGCCCAACTCAACCGGAAAAGAGGGGCGCGATTGTGCACGGaaa > 3:75769/8‑145 (MQ=255) aggccccaaGCGTTGAACAACGCTTTGGGCCTGCTCATTCATTCCGGGTGAAGCTCCACTCGCAAGACTGCAACAGGCTGAGAATGGCGAAATAGACGTTTCGCCCAACTCAACCGGAAAAGAGGGGCGCGATTGTGCACGGaaa < 4:75769/138‑1 (MQ=255) cTGCCAAGCGTTGTTCAACGCTTGGGGCCTGCTCATTCATTCCGGGTGAAGCTCCACTCGCAAGACTTCAACAGGCTGAGAATGGCGAAATAGACGTTTCGCCCAACTCAACCGGAAAAGAGGGGCGCGATTGTGCACGGAAAGGTTg < 7:287450/148‑1 (MQ=255) gCAAAGCGTTGTTCAACGCTTGGGGCCTGCTCATTCATTCCGGGTGAAGCTCCACTCGCAAGACTGCAACAGGCTGAGAATGGCGAAATAGACGTTTCGCCCAACTCAACCGGAAAAGAGGGGCGCGATTGTGCACGGAAAGGTTGCAc > 2:190861/1‑149 (MQ=255) cAAATCGTTGTTCAACGCTTGGTGCCAGCTCATTCATTCCGGGTGAAGCTCCACTCTCAAGACTGCAACAGGCTGAGAATGGCGAAAAAGACGTTTCGCCAAACACAACCTGAAAAGAGGGGCGCGAATGTGCACGGAAAGGTTGCACt > 2:298557/1‑149 (MQ=255) caaGCGTTGAACAACGCTTGGGGCCTGCTCATTCATTCCGGGTGAAGCTCCACTCGCAAGACTGCAACAGGCTGAGAATGGCGAAATAGACGTTTCGCCCAACTCAACCGGAAAAGAGGGGCGCGATTGTGCACGGAAAGGTTGCACtt > 5:342483/2‑149 (MQ=255) gttgttCAACGCTTGGGGCCTGCTCATTCATTCCGGGTGAAGCTCCACTCGCAAGACTGCAACAGGCTGAGAATGGCGAAATAGACGTTTCGCCCAACTCAACCGGAAAAGAGGGGCGCGATTGTGCACGGAAAGGTTGCACTTGCGaa < 1:190861/149‑1 (MQ=255) | GCAGCGGCCAGAAGCCGCCAAGGCCGGCGTTGATCGGCAGGAACAGTGTCCCGGCGCCGATGGCTGTGCCGAACAGGCCCAGCATCCAGGTGGTGTCGTGACGCGCCCAGGTGCCGAGGGCGGC‑GGGGGTCGATTCTGCAAAGCGTTGTTCAACGCTTGGGGCCTGCTCATTCATTCCGGGTGAAGCTCCACTCGCAAGACTGCAACAGGCTGAGAATGGCGAAATAGACGTTTCGCCCAACTCAACCGGAAAAGAGGGGCGCGATTGTGCACGGAAAGGTTGCACTTGCGAA > NC_002947/4079022‑4079314 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |