Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,096,650 C→A 5.1% S86* (TCG→TAG) ‡ PP_5608 → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,096,6500CA 5.1% 257.9 / 3.1 79S86* (TCG→TAG) ‡PP_5608hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (37/38);  new base A (2/2);  total (39/40)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.75e-01

GCCGCCGAGGCCCAACAGGCCAAGCTTTCCGGGGTAACCCTGTACGCCACGCGCAAGAAGCTGCAGGTACGCAAATTCCCCAAACCCTGGATGCGCATGACTGCCTTCGGCATTACCGAGCAAACCTACAACAGCCGCTCGCGGCTCAAGCCAGCAGCGATCAAGCAGACGTACTTCGAGCAGTGCGTTCAGCATGCCGTTGCAAGACGATAACTCTTTACTTATCACGCCTGCAAAGGCCGCATCAGCGTCTCCATGGAGGGCCTGCCTTGAAGCGATCTTCCAGC  >  NC_002947/4096511‑4096797
                                                                                                                                           |                                                                                                                                                   
gccgccGAGGCCCAACAGGCCAAGCTTTCCGGGGTAACCCTGTACGCCACGCGCAAGAAGCTGCAGGTACGCAAATTCCCCAAACCCTGGATGCGCATGACTGCCTTCGGCATTACCGAGCAAACCTACAACAGCCGCTCGCGGCTCaa                                                                                                                                            <  2:92617/149‑1 (MQ=255)
 ccgccgAGGCCCAACAGGCCAAGCTTTCCGGGGTAACCCTGTACGCCACGCGCAAGAAGCTGCAGGTACGCAAATTCTCCAAACCCTGGATGCGCATGACTGCCTTCGGCATTACCGAGCAAACCTACAACAGCCGCTCGCGGCTCAAg                                                                                                                                           >  1:144526/1‑149 (MQ=255)
 ccgccgAGGCCCAACAGGCCAAGCTTTCCGGGGTAACCCTGTACGCCACGCGCAAGAAGCTGCAGGTACGCAAATTCCCCAAACCCTGGATGCGCATGACTGCCTTCGGCATTACCGAGCAAACCTACAACAGCCGCTCGCGGCTCAAg                                                                                                                                           >  4:97378/1‑149 (MQ=255)
  cgccgAGGCCCAACAGGCCAAGCTTTCCGGGGTAACCCTGTACGCCACGCGCAAGAAGCTGCAGGTACGCAAATTCCCCAAACCCTGGATGCGCATGACTGCCTTCGGCATTACCGAGCAAACCTACAACAGCCGCTCGCGGCTCAAGc                                                                                                                                          <  3:122891/149‑1 (MQ=255)
   gccgAGGCCCAACAGGCCAAGCTTTCCGGGGTAACCCTGTACGCCACGCGCAAGAAGCTGCAGGTACGCAAATTCCCCAAACCCTGGATGCGCATGACTGCCTTCGGCATTACCGAGCAAACCTACAACAGCCGCGAGCGGCtgttgta                                                                                                                                         <  2:30538/149‑7 (MQ=255)
       aGGCCCAACAGGCCAAGCTTTCCGGGGTAACCCTGTACGCCACGCGCAAGAAGCTGCAGGTACGCAAATTCCCCAAACCCTGGATGCGCATGACTGCCTTCGGCATTACCGAGCAAACCTACAACAGCCGCTCGCGGCTCAAGCcagca                                                                                                                                     <  4:266906/149‑1 (MQ=255)
            cAACAGGCCAAGCTTTCCGGGGTAACCCTGTACGCCACGCGCAAGAAGCTGCAGGTACGCAAATTCTCCAAACCCTGGATGCGCATGACTGCCTTCGGCATTACCGAGCAAACCTACAACAGCCGCTCGCGGCTCAAGCCAGCAGCGAt                                                                                                                                <  2:144526/149‑1 (MQ=255)
                aGGCCAAGCTTTCCGGGGTAACCCTGTACGCCACGCGCAAGAAGCTGCAGGTACGCAAATTCCCCAAACCCTGGATGCGCATGACTGCCTTCGGCATTACCGAGCAAACCTACAACAGCCGCTCGCGGCTCAAGCCAGCAGCGATCAAg                                                                                                                            <  5:167759/149‑1 (MQ=255)
                aGGCCAAGCTTTCCGGGGTAACCCTGTACGCCACGCGCAAGAAGCTGCAGGTACGCAAATTCCCCAAACCCTGGATGCGCATGACTGCCTTCGGCATTACCGAGCAAACCTACAACAGCCGCTCGCGGCTCAAGCCAGCAGCGATCAAg                                                                                                                            <  3:68383/149‑1 (MQ=255)
                  gCCAAGCTTTCCGGGGTAACCCTGTACGCCACGCGCAAGAAGCTGCAGGTACGCAAATTCCCCAAACCCTGGATGCGCATGACTGCCTTCGGCATTACCGAGCAAACCTACAACAGCCGCTCGCGGCTCAAGCCAGCAGCGATCAAg                                                                                                                            >  2:74395/1‑147 (MQ=255)
                   ccAAGCTTTCCGGGGTAACCCTGTACGCCACGCGCAAGAAGCTGCAGGTACGCAAATTCCCCAAACCCTGGATGCGCATGACTGCCTTCGGCATTACCGAGCAAACCTACAACAGCCGCTCGCGGCTCAAGCCAGCAGCGATCAAg                                                                                                                            >  1:217242/1‑146 (MQ=255)
                   ccAAGCTTTCCGGGGTAACCCTGTACGCCACGCGCAAGAAGCTGCAGGTACGCAAATTCCCCAAACCCTGGATGCGCATGACTGCCTTCGGCATTACCGAGCAAACCTACAACAGCCGCGAGCGGCtgttgt                                                                                                                                          >  1:257072/1‑127 (MQ=255)
                   ccAAGCTTTCCGGGGTAACCCTGTACGCCACGCGCAAGAAGCTGCAGGTACGCAAATTCCCCAAACCCTGGATGCGCATGACTGCCTTCGGCATTACCGAGCAAACCTACAACAGCCGCGAGCGGCtgttgt                                                                                                                                          <  2:257072/132‑6 (MQ=255)
                      aGCTTTCCGGGGTAACCCTGTACGCCACGCGCAAGAAGCTGCAGGTACGCAAATTCCCCAAACCCTGGATGCGCATGACTGCCTTCGGCATTACCGAGCAAACCTACAACAGCCGCTCGCGGCTCAAGCCAGCAGCGATCAAGCAGACg                                                                                                                      <  4:324473/149‑1 (MQ=255)
                           tCCGGGGTAACCCTGTACGCCACGCGCAAGAAGCTGCAGGTACGCAAATTCCCCAAACCCTGGATGCGCATGACTGCCTTCGGCATTACCGAGCAAACCTACAACAGCCGCTCGCGGCTCAAGCCAGCAGCGATCAAGCAGACGTACt                                                                                                                  <  8:289800/148‑1 (MQ=255)
                            ccGGGGTAACCCTGTACGCCACGCGCAAGAAGCTGCAGGTACGCAAATTCCCCAAACCCTGGATGCGCATGACTGCCTTCGGCATTACCGAGCAAACCTACAACAGCCGCTCGCGGCTCAAGCCAGCAGCGATCAAGCAGACGTACTTc                                                                                                                >  2:338565/1‑149 (MQ=255)
                             cGGGGTAACCCTGTACGCCACGCGCAAGAAGCTGCAGGTACGCAAATTCCCCAAACCCTGGATGCGCATGACTGCCTTCGGCATTACCGAGCAAACCTACAACAGCCGCTcgc                                                                                                                                                   <  6:191415/113‑1 (MQ=255)
                             cGGGGTAACCCTGTACGCCACGCGCAAGAAGCTGCAGGTACGCAAATTCCCCAAACCCTGGATGCGCATGACTGCCTTCGGCATTACCGAGCAAACCTACAACAGCCGCTcgc                                                                                                                                                   >  5:191415/1‑113 (MQ=255)
                               gggTAACCCTGTACGCCACGCGCAAGAAGCTGCAGGTACGCAAATTCCCCAAACCCTGGATGCGCATGACTGCCTTCGGCATTACCGAGCAAACCTACAACAGCCGCTCGCGGCTCAAGCCAGCAGCGATCAAGCAGACGTACTTCGAg                                                                                                             <  1:234156/149‑1 (MQ=255)
                                 gTAACCCTGTACGCCACGCGCAAGAAGCTGCAGGTACGCAAATTCCCCAAACCCTGGATGCGCATGACTGCCTTCGGCATTACCGAGCAAACCTACAACAGCCGCTCGCGGCTCAAGCCAGCAGCGATCAAGCAGACGTACTTCGAg                                                                                                             >  3:7312/1‑147 (MQ=255)
                                          tACGCCACGCGCAAGAAGCTGCAGGTACGCAAATTCCCCAAACCCTGGATGCGCATGACTGCCTTCGGCATTACCGAGCAAACCTACAACAGCCGCTCGCGGCTCAAGCCAGCAGCGATCAAGCAGACGTACTTCGAGCAGTGCGTTc                                                                                                   <  4:274781/148‑1 (MQ=255)
                                          tACGCCACGCGCAAGAAGCTGCAGGTACGCAAATTCCCCAAACCCTGGATGCGCATGACTGCCTTCGGCATTACCGAGCAAACCTACAACAGCCGCTCGCGGCTCAAGCCAGCAGCGATCAAGCAGACGTACTTCGAGCAGTGCGTTCa                                                                                                  <  3:286308/149‑1 (MQ=255)
                                               cACGCGCAAGAAGCTGCAGGTACGCAAATTCCCCAAACCCTGGATGCGCATGACTGCCTTCGGCATTACCGAGCAAACCTACAACAGCCGCTCGCGGCTCAAGCCAGCAGCGATCAAGCAGACGTACTTCGAGCAGTGCGTTCAGCATg                                                                                             >  1:48664/1‑149 (MQ=255)
                                                  gcgcAAGAAGCTGCAGGTACGCAAATTCCCCAAACCCTGGATGCGCATGACTGCCTTCGGCATTACCGAGCAAACCTACAACAGCCGCTCGCGGCTCAAGCCAGCAGCGATCAAGCAGACGTACTTCGAGCAGTGCGTTCAGCATGCCg                                                                                          >  8:183262/1‑149 (MQ=255)
                                                    gcAAGAAGCTGCATGTACGCAAATTCCCCAATCCCTGGATGCGCATGACTGCCTTCGGCATTACCGAGCAAACCTACAACAGCCGCTCGCGGCTCAAGCCAGCAGCGATCAAGCAGACGTACTTCGAGCAGTGCGTTCAGCATGCCGtt                                                                                        <  1:301577/149‑1 (MQ=255)
                                                    gcAAGAAGCTGCAGGTACGCAAATTCCCCAAACCCTGGATGCGCATGACTGCCTTCGGCATTACCGAGCAAACCTACAACAGCCGCTCGCGGCTCAAGCCAGCAGCGATCAAGCAGACGTACTTCGAGCAGTGCGTTCAGCATGCCGtt                                                                                        >  1:139275/1‑149 (MQ=255)
                                                     cAAGAAGCTGCAGGTACGCAAATTCCCCAAACCCTGGATGCGCATGACTGCCTTCGGCATTACCGAGCAAACCTACAACAGCCGCTCGCgg                                                                                                                                                 >  7:278927/1‑91 (MQ=255)
                                                     cAAGAAGCTGCAGGTACGCAAATTCCCCAAACCCTGGATGCGCATGACTGCCTTCGGCATTACCGAGCAAACCTACAACAGCCGCTCGCgg                                                                                                                                                 <  8:278927/91‑1 (MQ=255)
                                                      aagaagCTGCAGGTACGCAAATTCCCCAAACCCTGGATGCGCATGACTGCCTTCGGCATTACCGAGCAAACCTACAACAGCCGCTCGCGGCTCAAGCCAGCAGCGATCAAGCAGACGTACTTCGAGCAGt                                                                                                         >  3:189116/1‑130 (MQ=255)
                                                      aagaagCTGCAGGTACGCAAATTCCCCAAACCCTGGATGCGCATGACTGCCTTCGGCATTACCGAGCAAACCTACAACAGCCGCTCGCGGCTCAAGCCAGCAGCGATCAAGCAGACGTACTTCGAGCAGt                                                                                                         <  4:189116/130‑1 (MQ=255)
                                                       agaagCTGCAGGTACGCAAATTCCCCAAACCCTGGATGCGCATGACTGCCTTCGGCATTACCGAGCAAACCTACAACAGCCGCTCGCGGCTCAAGCCAGCAGCGATCAAGCAGACGTACTTCGAGCAGTGCGTTCAGCATGCCGTTGc                                                                                      >  1:125902/1‑148 (MQ=255)
                                                       agaagCTGCAGGTACGCAAATTCCCCAAACCCTGGATGCGCATGACTGCCTTCGGCATTACCGAGCAAACCTACAACAGCCGCTCGCGGCTCAAGCCAGCAGCGATCAAGCAGACGTACTTCGAGCAGTGCGTTCAGCATGCCGTTGCa                                                                                     <  1:74395/149‑1 (MQ=255)
                                                          agCTGCAGGTACGCAAATTCCCCAAACCCTGGATGCGCATGACTGCCTTCGGCATTACCGAGCAAACCTACAACAGCCGCTCGCGGCTCAAGCCAGCAGCGATCAAGCAGACGTACTTCGAGCAGTGCGTTCAGCATGCCGTTGCAAGa                                                                                  <  3:97378/149‑1 (MQ=255)
                                                                aGGTACGCAAATTCCCCAAACCCTGGATGCGCATGACTGCCTTCTGCATTACCGAGCAAACCTACAACAGCCGCTCGCGGCTCAAGCCAGCAGCGATCAAGAAGACGTACATCGAGCAGTGCTTTCAGCATGCCGTTGCAAGACGATa                                                                             >  5:140905/1‑148 (MQ=255)
                                                                aGGTACGCAAATTCCCCAAACCCTGGATGCGCATGACTGCCTTCGGCATTACCGAGCAAACCTACAACAGCCGCTCGCGGCTCAAGCCAGCAGCGATCAAGCAGACGTACTTCGAGCAGTGCGTTCAGCAt                                                                                              <  5:9193/131‑1 (MQ=255)
                                                                aGGTACGCAAATTCCCCAAACCCTGGATGCGCATGACTGCCTTCGGCATTACCGAGCAAACCTACAACAGCCGCTCGCGGCTCAAGCCAGCAGCGATCAAGCAGACGTACTTCGAGCAGTGCGTTCAGCAt                                                                                              >  6:9193/1‑131 (MQ=255)
                                                                aGGTACGCAAATTCCCCAAACCCTGGATGCGCATGACTGCCTTCGGCATTACCGAGCAAACCTACAACAGCCGCTCGCGGCTCAAGCCAGCAGCGATCAAGCAGACGTACTTCGAGCAGTGCGTTCAGCATGCCGTTGCAAGACGATaa                                                                            >  4:61324/1‑149 (MQ=255)
                                                                  gTACGCAAATTCCCCAAACCCTGGATGCGCATGACTGCCTTCGGCATTACCGAGCAAACCTACAACAGCCGCTCGCGGCTCAAGCCAGCAGCGATCAAGCAGACGTACTTCGAGTAGTGCGTTCAGCATGCCGTTGCAAGACGATAAct                                                                          <  1:51622/149‑1 (MQ=255)
                                                                   tACGCAAATTCCCCAAACCCTGGATGCGCATGACTGCCTTCGGCATTACCGAGCAAACCTACAACAGCCGCTCGCGGCTCAAGCCAGCAGCGATCAAGCAGACGTACTTCGAGCAGTGCGTTCAGCATGCCGTTGCAAGACGATAActc                                                                         >  6:265289/1‑149 (MQ=255)
                                                                     cGCAAATTCCCCAAACCCTGGATGCGCATGACTGCCTTCGGCATTACCGAGCAAACCTACAACAGCCGCTCGCGGCTCAAGCCAGCAGCGATCAAGCAGACGTACTTCGAGCAGTGCGTTCAGCATGCCGTTGCAAGACGATAACTCtt                                                                       <  2:125902/149‑1 (MQ=255)
                                                                     cGCAAATTCCCCAAACCCTGGATGCGCATGACTGCCTTCGGCATTACCGAGCAAACCTACAACAGCCGCTCGCGGCTCAAGCCAGCAGCGATCAAGCAGACGTACTTCGAGCAGTGCGTTCAGCATGCCGTTGCAAGACGATAACTCtt                                                                       >  3:223978/1‑149 (MQ=255)
                                                                       cAAATTCCCCAAACCCTGGATGCGCATGACTGCCTTCGGCATTACCGAGCAAACCTACAACAGCCGCTCGCGGCTCAAGCCAGCAGCGATCAAGCAGACGTACTTCGAGCAGTGCGTTCAGCATGCCGTTGCAAGACGATAACTCTTTa                                                                     <  6:68949/149‑1 (MQ=255)
                                                                          aTTCCCCAAACCCTGGATGCGCATGACTGCCTTCGGCATTACCGAGCAAACCTACAACAGCCGCTCGCGGCTCAAGCCAGCAGCGATCAAGCAGACGTACTTCGAGCAGTGCGTTCAGCATGCCGTTGCAAGACGATAACTCTTTACtt                                                                  <  7:183262/149‑1 (MQ=255)
                                                                              cccAAACCCTGGATGCGCATGACTGCCTTCGGCATTACCGAGCAAACCTACAACAGCCGCTCGCGGCTCAAGCCAGCAGCGATCAAGCAGACGTACTTCGAGCAGTGCGTTCAGCATGCCGTTGCAAGACGATAACTCTTTACTTATc                                                               <  4:7312/148‑1 (MQ=255)
                                                                              cccAAACCCTGGATGCGCATGACTGCCTTCGGCATTACCGAGCAAACCTACAACAGCCGCTCGCGGCTCAAGCCAGCAGCGATCAAGCAGACGTACTTCGAGCAGTGCGTTCAGCATGCCGTTGCAAGACGATAACTCTTTACTTATCa                                                              >  5:197807/1‑149 (MQ=255)
                                                                               ccAAACCCTGGATGCGCATGACTGCCTTGGGCATTACCGAGCAAACCTACAACAGCCGCTCGCGGCTCAAGCCAGCAGCGATCAAGCAGACGTACTTCGAGCAGTGCGTTCAGCATGCCGTTGCAAGACGATAACTCTTTACTTATCAc                                                             >  1:189627/1‑149 (MQ=255)
                                                                               ccAAACCCTGGATGCGCATGACTGCCTTCGGCATTACCGAGCAAACCTACAACAGCCGCTCGCGGCTCAAGCCAGCAGCGATCAAGCAGACGTACTTCGAGCAGTGCGTTCAGCATGCCGTTGCAAGACGATAACTCTTTACTTATCAc                                                             >  6:54171/1‑149 (MQ=255)
                                                                               ccAAACCCTGGATGCGCATGACTGCCTTCGGCATTACCGAGCAAACCTACAACAGCCGCTCGCGGCTCAAGCCAGCAGCGATCAAGCAGACGTACTTCGAGCAGTGCGTTCAGCATGCCGTTGCAAGACGATAACTCTTTACTTATCAc                                                             >  7:93702/1‑149 (MQ=255)
                                                                                 aaaCCCTGGATGCGCATGACTGCCTTCGGCATTACCGAGCAAACCTACAACAGCCGCTCGCGGCTCAAGCCAGCAGCGATCAAGCAGACGTACTTCGAGCAGTGCGTTCAGCATGCCGTTGCAAGACGATAACTCTTTACTTATCACGc                                                           <  8:93702/149‑1 (MQ=255)
                                                                                   aCCCTGGATGCGCATGACTGCCTTCGGCATTACCGAGCAAACCCACAACAGCCGCTCGCGGCTCAAGCCAGCAGCGATCAAGCAGACGTACTTCGAGCAGTGCGTTCAGCATGCCGTTGCAAGACGATAACTCTTTACTTATCACGCCt                                                         >  7:261421/1‑149 (MQ=255)
                                                                                    cccTGGATGCGCATGACTGCCTTCGGCATTACCGAGCAAACCTACAACAGCCGCTCGCGGCTCAAGCCAGCAGCGATCAAGCAGACGTACTTCGAGCAGTGCGTTCAGCATGCCGTTGCAAGACGATAACTCTTTACTTATCACGCCTg                                                        <  2:139275/149‑1 (MQ=255)
                                                                                     ccTGGATGCGCATGACTGCCTTCGGCATTACCGAGCAAACCTACAACAGCCGCTCGCGGCTCAAGCCAGCAGCGATCAAGCAGACGTACTTCGAGCAGTGCGTTCAGCATGCCGTTGCAAGACGATAACTCTTTACTTATCACGCCTg                                                        >  6:226971/1‑148 (MQ=255)
                                                                                         gATGCGCATGACTGCCTTCGGCATTACCGAGCAAACCTACAACAGCCGCTCGCGGCTCAAGCCAGCAGCGATCAAGCAGACGTACTTCGAGCAGTGCGTTCAGCATGCCGTTGCAAGACGATAACTCTTTACTTATCACGCCTGCaaa                                                    <  3:61324/148‑1 (MQ=255)
                                                                                              gcATGACTGCCTTCGGCATTACCGAGCAAACCTACAACAGCCGCTCGCGGCTCAAGCCAGCAGCGATCAAGCAGACGTACTTCGAGCAGTGCGTTCAGCATGCCGTTGCAAGACGATAACTCTTTACTTATCACGCCTGCAAAGGCCg                                               >  8:99027/1‑148 (MQ=255)
                                                                                                  gACTGCCTTCGGCATTACCGAGCAAACCTACAACAGCCGCTCGCGGCTCAAGCCAGCAGCGATCAAGCAGACGTACTTCGAGCAGTGCGTTCAGCATGCCGTTGCAAGACGATAACTCTTTa                                                                     >  4:212630/1‑122 (MQ=255)
                                                                                                  gACTGCCTTCGGCATTACCGAGCAAACCTACAACAGCCGCTCGCGGCTCAAGCCAGCAGCGATCAAGCAGACGTACTTCGAGCAGTGCGTTCAGCATGCCGTTGCAAGACGATAACTCTTTa                                                                     <  3:212630/122‑1 (MQ=255)
                                                                                                   aCTGCCTTCGGCATTACCGAGCAAACCTACAACAGCCGCTCGCGGCTCAAGCCAGCAGCGATCAAGCAGACGTACTTCGAGCAGTGCGTTCAGCATGCCGTTGCAAGACGATAACTCTTTACTTATCACGCCTGCAAAGGCCGCATCa                                          <  2:217242/148‑1 (MQ=255)
                                                                                                    cTGCCTTCGGCATTACCGAGCAAACCTACAACAGCCGCTCGCGGCTCAAGCCAGCAGCGATCAAGCAGACGTACTTCGAGCAGTGCGTTCAGCATGCCGTTGCAAGACGATAACTCTTTACTTATCACGCCTGCAAAGGCCGCATCAGc                                        <  5:265289/149‑1 (MQ=255)
                                                                                                    cTGCCTTCGGCATTACCGAGCAAACCTACAACAGCCGCTCGCGGCTCAAGCCAGCAGCGATCAAGCAGACGTACTTCGAGCAGTGCGTTCAGCATGCCGTTGCAAGACGATAACTCTTTACTTATCACGCCTGCAAAGGCCGCATCAGc                                        >  5:173228/1‑149 (MQ=255)
                                                                                                    cTGCCTTCGGCATTACCGAGCAAACCTACAACAGCCGCTCGCGGCTCAAGCCAGCAGCGATCAAGCAGACGTACTTCGAGCAGTGCGTTCAGCATGCCGTTGCAAGACGATAACTCTTTACTTATCACGCCTGCAAAGGCCGCATCAGc                                        <  7:99027/149‑1 (MQ=255)
                                                                                                        cTTCGGCATTACCGAGCAAACCTACAACAGCCGCTCGCGGCTCAAGCCAGCAGCGATCAAGCAGACGTACTTCGAGCAGTGCGTTCAGCATGCCGTTGCAAGACGATAACTCTTTACTTATCACGCCTGCAAAGGCCGCATCAGCGtct                                    <  1:338565/149‑1 (MQ=255)
                                                                                                         ttCGGCATTACCGAGCAAACCTACAACAGCAGCTCGCGGCTCAAGCCAGCAGCGATCAAGCAGCCGTACTTCGAGCAGTGCGTTCAGCATGCCGTTg                                                                                       >  5:97236/1‑97 (MQ=255)
                                                                                                            ggCATTACCGAGCAAACCTACAACAGCCGCTCGCGGCTCAAGCCAGCAGCGATCAAGCAGACGTACTTCGAGCAGTGCGTTCAGCATGCCGTTGCAAGACGATAACTCTTTACTTATCACGCCTGCAAAGGCCGCATCAGCGTCTCCAt                                <  6:197807/149‑1 (MQ=255)
                                                                                                                ttACCGAGCAAACCTACAACAGCCGCTCGCGGCTCAAGCCAGCAGCGATCAAGCAGACGTACTTCGAGCAGTGCGTTCAGCATGCCGTTGCAAGACGATAACTCTTTACTTATCACGCCTGCAAAGGCCGCATCAGCGTCTCCATGGAg                            <  2:48664/149‑1 (MQ=255)
                                                                                                                  aCCGAGCAAACCTACAACAGCCGCTCGCGGCTCAAGCCAGCAGCGATCAAGCAGACGTACTTCGAGCAGTGCGTTCAGCATGCCGTTGCAAGACGATAACTCtt                                                                       >  7:145323/1‑104 (MQ=255)
                                                                                                                  aCCGAGCAAACCTACAACAGCCGCTCGCGGCTCAAGCCAGCAGCGATCAAGCAGACGTACTTCGAGCAGTGCGTTCAGCATGCCGTTGCAAGACGATAACTCtt                                                                       <  8:145323/104‑1 (MQ=255)
                                                                                                                   ccGAGCAAACCTACAACAGCCGCTCGCGGCTCAAGCCAGCAGCGATCAAGCAGACGTACTTCGAGCAGTGCGTTCAGCATGCCGTTGCAAGACGATAACTCTTTACTTATTACGCCTGCAAAGGCCGCATCAGCGTCTCCATGGAGGgc                         >  4:266526/1‑149 (MQ=255)
                                                                                                                   cagAGCAAACCTACAACAGCCGCTCGCGGCTCAAGCCAGCAGCGATCAAGCAGACGTACTTCGAGCAGTGCGTTCAGCATGCCGTTGCAAGACGATAACTCTTTACTTATCACGCCTGCAAAGGCCGCATCAGCGtc                                     >  7:226234/3‑137 (MQ=255)
                                                                                                                   cagAGCAAACCTACAACAGCCGCTCGCGGCTCAAGCCAGCAGCGATCAAGCAGACGTACTTCGAGCAGTGCGTTCAGCATGCCGTTGCAAGACGATAACTCTTTACTTATCACGCCTGCAAAGGCCGCATCAGCGtc                                     <  8:226234/135‑1 (MQ=255)
                                                                                                                         aaaCCTACAACAGCCGCTCGCGGCTCAAGCCAGCAGCGATCAAGCAGACGTACTTCGAGCAGTGCGTTCAGCATGCCGTTGCAAGACGATAACTCTTTACTTATCACGCCTGCAAAGGCCGCATCAg                                         <  4:205243/127‑1 (MQ=255)
                                                                                                                             tggcttgaGCCGCGAGCGGCTCAAGCCAGCAGCGATCAAGCAGACGTACTTCGAGCAGTGCGTTCAGCATGCCGTTGCAAGACGAAAACTCTTTACTTATCAAGCATGCAAAGGAAGCATCAGCGTCTCCATGGAGGGCCTCCCTTGaa               >  2:1266/8‑149 (MQ=255)
                                                                                                                             cTACAACAGCCGCTCGCGGCTCAAGCCAGCAGCGATCAAGCAGACGTACTTCGAGCAGTGCGTTCAGCATGCCGTTGCAAGACGATAACTCTTTACTTATCACGCCTGCAAAGGCCGCATCAGCGTCTCCATGGAGGGCCTGCCTTg                 >  5:172039/1‑147 (MQ=255)
                                                                                                                             cTACAACAGCCGCTCGCGGCTCAAGCCAGCAGCGATCAAGCAGACGTACTTCGAGCAGTGCGTTCAGCATGCCGTTGCAAGACGATAACTCTTTACTTATCACGCCTGCAAAGGCCGCATCAGCGTCTCCATGGAGGGCCTGCCTTg                 >  5:332743/1‑147 (MQ=255)
                                                                                                                             cTACAACAGCCGCTCGCGGCTCAAGCCAGCAGCGATCAAGCAGACGTACTTCGAGCAGTGCGTTCAGCATGCCGTTGCAAGACGATAACTCTTTACTTATCACGCCTGCAAAGGCCGCATCAGCGTCTCCATGGAGGGCCTGCCTTGaa               <  5:54171/149‑1 (MQ=255)
                                                                                                                              tACAACAGCCGCTCGCGGCTCAAGCCAGCAGCGATCAAGCAGACGTACTTCGAGCAGTGCGTTCAGCATGACGTTGCAAGACGATAACTCTTTACTTATCACGCCTGCAAAGGCCTCATAAGCGTCTCCATGGAGGgactggctgg                 >  2:195910/1‑144 (MQ=255)
                                                                                                                                 aacaGCCGCTCGCGGCTCAAGCCAGCAGCGATCAAGCAGACGTACTTCGAGCAGTGCGTTCAGCATGCCGTTGCAAGACGATAACTCTTTACTTATCACGCCTGCAAAGGCCGCATCAGCGTCTCCATGGAGGGCCTGCCTTGAAGCGa           >  2:1404/1‑149 (MQ=255)
                                                                                                                                  acaGCCGCTCGCGGCTCAAGCCAGCAGCGATCAAGCAGACGTACTTCGAGCAGTGCGTTCAGCATGCCGTTGCAAGACGATAACTCTTTACTTATCACGCCTGGAAAGGCCGCATCAGCGTCTCCATGGAGGGCCTGCCTTGAAGCGAt          <  5:226971/149‑1 (MQ=255)
                                                                                                                                    aGCCGCTCGCGGCTCAAGCCAGCAGCGATCAAGCAGACGTACTTCGAGCAGTGCGTTCAGCATGCCGTTGCAAGACGATAACTCTTTACTTATCACGCCTGCAAAGGCCGCATCAGCGTCTCCATGGAGGGCCTGCCTTGAAGCGATCt        <  2:189627/149‑1 (MQ=255)
                                                                                                                                         cTCGCGGCTCAAGCCAGCAGCGATCAAGCAGACGTACTTCGAGCAGTGCGTTCAGCATGCCGTTGCTAGACGATAACTCTTTACTTATCACGCCTGCAAAGGCCGCATCAGCGTCTCCATGGAGGGCCTGCCTTGAAGCGATCTTccag   >  1:219074/1‑149 (MQ=255)
                                                                                                                                          tCGCGGCTCAAGCCAGCAGCGATCAAGCAGACGTACTTCGAGCAGTGCGTTCAGCATGCCGTTGCAAGACGATAACTCTTTACTTATCACGCCTGCAAAGGCCGCATCAGCGTCTCCATGGAGGGCCTGCCTTGAAGCGATCTTccagc  >  3:318355/1‑149 (MQ=255)
                                                                                                                                          tCGCGGCTCAAGCCAGCAGCGATCAAGCAGACGTACTTCGAGCAGTGCGTTCAGCATGCCGTTGCAAGACGATAACTCTTTACTTATCACGCCTGCAAAGGCCGCATCAGCGTCTCCATGGAGGGCCTGCCTTGAAGCGATCTTccagc  >  3:323435/1‑149 (MQ=255)
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GCCGCCGAGGCCCAACAGGCCAAGCTTTCCGGGGTAACCCTGTACGCCACGCGCAAGAAGCTGCAGGTACGCAAATTCCCCAAACCCTGGATGCGCATGACTGCCTTCGGCATTACCGAGCAAACCTACAACAGCCGCTCGCGGCTCAAGCCAGCAGCGATCAAGCAGACGTACTTCGAGCAGTGCGTTCAGCATGCCGTTGCAAGACGATAACTCTTTACTTATCACGCCTGCAAAGGCCGCATCAGCGTCTCCATGGAGGGCCTGCCTTGAAGCGATCTTCCAGC  >  NC_002947/4096511‑4096797

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑