Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	4,138,585	C→A	8.5%	G28G (GGC→GGA)	PP_3643 →	oxidoreductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	4,138,585	0	C	A	8.5%	128.9 / 2.1	43	G28G (GGC→GGA)	PP_3643	oxidoreductase
Reads supporting (aligned to +/- strand):  ref base C (21/18);  new base A (2/2);  total (23/20)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.17e-01

GTATACCATCAGACACATCAACGATAAAAACCGCGGACCACCGCAGCCCTTCAGAGGTACTCCCGATGATCGACGCAACTGTCTACAAGAACGTTCTGGGCTCCTTCCCGTCCGGCGTTACCGTCATCACCACCCAGGACGACGACGGCTCGGTCGTCGGCCTGACCGCCAGCGCCTTCTCCTCCCTGTCGATGGACCCGCCGCTGGTGCTGTTCTGCCCCAACTACACCTCCGACTCCTACCCGGTACTGATCAAGCAGAAGCGCTTTGCCATTCACCTGCTGTCCGGTG  >  NC_002947/4138437‑4138727                                                                                                                                                     |                                                                                                                                               gTATACCATCAGACACATCAACGATAAAAACCGCGGACCACCGCAGCCCTTCAGAGGTACTCCCGATGATCGACGCAACTGTCTACAAGAACGTTCTGGGCTCCTTCCCGTCCGGCGTTACCGTCATCACCACCCAGGACGACGACGGc                                                                                                                                                >  8:251486/1‑149 (MQ=255)     aCCATCAGACACATCAACGATAAAAACCGCGGACCACCGCAGCCCTTCAGAGGTACTCCCGATGATCGACGCAACTGTCTACAAGAACGTTCTGGGCTCCTTCCCGTCCGGCGTTACCGTCATCACCACCCAGGACGACGACGGCTCgg                                                                                                                                            >  4:159824/1‑149 (MQ=255)             acacATCAACGATAAAAACCGCGGACCACCGCAGCCCTTCAGAGGTACTCCCGATGATCGACGCAACTGTCTACAAGAACGTTCTGGGCTCCTTCCCGTCCGGCGTTACCGTCATCACCACCCAGGACGACGACGGCTCGGTCGTCGGc                                                                                                                                    <  6:134589/149‑1 (MQ=255)             acacATCAACGATAAAAACCGCGGACCACCGCAGCCCTTCAGAGGTACTCCCGATGATCGACGCAACTGTCTACAAGAACGTTCTGGGCTCCTTCCCGTCCGGCGTTACCGTCATCACCACCCAGGACGACGACGGCTCGGTCGTCGGc                                                                                                                                    <  3:68967/149‑1 (MQ=255)                 aTCAACGATAAAAACCGCGGACCACCGCAGCCCTTCAGAGGTACTCCCGATGATCGACGCAACTGTCTACAAGAACGTTCTGGGCTCCTTCCCGTCCGGCGTTACCGTCATCACCACCCAGGCCGACGACCGAGCCGTCGTCGTCCTgg                                                                                                                                <  3:313149/149‑2 (MQ=255)                 aTCAACGATAAAAACCGCGGACCACCGCAGCCCTTCAGAGGTACTCCCGATGATCGACGCAACTGTCTACAAGAACGTTCTGGGCTCCTTCCCGTCCGGCGTTACCGTCATCACCACCCAGGACGACGACGGCTCGGTCGTCGGCCTGa                                                                                                                                <  1:148598/149‑1 (MQ=255)                 aTCAACGATAAAAACCGCGGACCACCGCAGCCCTTCAGAGGTACTCCCGATGATCGACGCAACTGTCTACAAGAACGTTCTGGGCTCCTTCCCGTCCGGCGTTACCGTCATCACCACCCAGGACGACGACGGCTCGGTCGTCGGCCTGa                                                                                                                                >  8:146207/1‑149 (MQ=255)                  tCAACGATAAAAACCGCGGACCACCGCAGCCCTTCAGAGGTACTCCCGATGATCGACGCAACTGTCTACAAGAACGTTCTGGGCTCCTTCCCGTCCGGCGTTACCGTCATCACCACCCAGGACGACGACGGc                                                                                                                                                <  1:226987/132‑1 (MQ=255)                  tCAACGATAAAAACCGCGGACCACCGCAGCCCTTCAGAGGTACTCCCGATGATCGACGCAACTGTCTACAAGAACGTTCTGGGCTCCTTCCCGTCCGGCGTTACCGTCATCACCACCCAGGACGACGACGGc                                                                                                                                                >  2:226987/1‑132 (MQ=255)                  tCAACGATAAAAACCGCGGACCACCGCAGCCCTTCAGAGGTACTCCCGATGATCGACGCAACTGTCTACAAGAACGTTCTGGGCTCCTTCCCGTCCGGCGTTACCGTCATCACCACCCAGGACGACGACGGCTCGGTCGTCGGCCTGAc                                                                                                                               >  6:281058/1‑149 (MQ=255)                    aaCGATAAAAACCGCGGACCACCGCAGCCCTTCAGAGGTACTCCCGATGATCGACGCAACTGTCTACAAGAACGTTCTGGGCTCCTTCCCGTCCGGCGTTACCGATCTCACCACCCAGGACGACGACGGCTCGGTCGTCGGCCTGACCg                                                                                                                             >  1:115043/1‑149 (MQ=255)                      cGATAAAAACCGCGGACCACCGCAGCCCTTCAGGGGTACTCCCGATGATCGACGCAACTGTCTACAAGAACGTTCTGGGCTCCTTCCCGTCCGGCGTTACCGTCATCACCACCCAGGACGACGACGGCTCGGTCGTCGGCCTGACCGcc                                                                                                                           <  2:104460/149‑1 (MQ=255)                         tAAAAACCGCGGACCACCGCAGCCCTTCAGAGGTACTCCCGATGATCGACGCAACTGTCTACAAGAACGTTCTGGGCTCCTTCCCGTCCGGCGTTACCGTCATCACCACCCAGGACGACGACGGCTCGGTCGTCGGCCTGa                                                                                                                                <  6:75867/141‑1 (MQ=255)                         tAAAAACCGCGGACCACCGCAGCCCTTCAGAGGTACTCCCGATGATCGACGCAACTGTCTACAAGAACGTTCTGGGCTCCTTCCCGTCCGGCGTTACCGTCATCACCACCCAGGACGACGACGGCTCGGTCGTCGGCCTGa                                                                                                                                >  5:75867/1‑141 (MQ=255)                         tAAAAACCGCGGACCACCGCAGCCCTTCAGAGGTACTCCCGATGATCGACGCAACTGTCTACAAGAACGTTCTGGGCTCCTTCCCGTCCGGCGTTACCGTCATCACCACCCAGGACGACGACGGCTCGGTCGTCGGCCTGACCGCCAgc                                                                                                                        <  7:251486/149‑1 (MQ=255)                              aCCGCGGACCACCGCAGCCCTTCAGAGGTACTCCCGATGATCGACGCAACTGTCTACAAGAACGTTCTGGGCTCCTTCCCGTCCGGCGTTACCGTCATCACCACCCAGGACGACGACGGCTCGGTCGTCGGCCTGACCGCCAGCGCCtt                                                                                                                   <  7:146207/149‑1 (MQ=255)                                 gcgGACCACCGCAGCCCTTCAGAGGTACTCCCGATGATCGACGCAACTGTCTACAAGAACGTTCTGGGCTCCTTCCCGTCCGGCGTTACCGTCATCACCACCCAGGACGACGACGGCTCGGTCGTCGGCCTGACCGCCAGCGCCTtctc                                                                                                                <  5:281058/149‑1 (MQ=255)                                    gACCACCGCAGCCCTTCAGAGGTACTCCCGATGATCGACGCAACTGTCTACAAGAACGTTCTGGGCTCCTTCCCGTCCGGCGTTACCGTCATCACCCCCCAGGACGACGACGGCTCGGTCGTCGGCCTGCCCGCCAGCGCCTTctcctc                                                                                                             >  6:23659/1‑149 (MQ=255)                                         ccGCAGCCCTTCAGAGGTACTCCCGATGATCGACGCAACTGTCTACAAGAACGTTCTGGGCTCCTTCCCGTCCGGCGTTACCGTCATCACCACCCAGGACGACGACGGCTCGGTCGTCGGCCTGACCGCCAGCGCCTTCTCCTCCCTGt                                                                                                        >  5:153701/1‑149 (MQ=255)                                             aGCCCTTCAGAGGTACTCCCGATGATCGACGCAACTGTCTACAAGAACGTTCTGGGCTCCTTCCCGTCCGGCGTTACCGTCATCACCACCCAGGACGACGACGGCTCGGTCGTCGGCCTGACCGCCAGCGCCTTCTCCTCCCTGTCGAt                                                                                                    <  2:54710/149‑1 (MQ=255)                                                             tCCCGATGATCGACGCAACTGTCTACAAGAACGTTCTGGGCTCCTTCCCGTCCGGCGTTACCGTCATCACCACCCAGGACGACGACGGCTCGGTCGTCGGCCTGACCGCCAGCGCCTTCTCCTccc                                                                                                           <  2:108897/126‑1 (MQ=255)                                                             tCCCGATGATCGACGCAACTGTCTACAAGAACGTTCTGGGCTCCTTCCCGTCCGGCGTTACCGTCATCACCACCCAGGACGACGACGGCTCGGTCGTCGGCCTGACCGCCAGCGCCTTCTCCTccc                                                                                                           >  1:108897/1‑126 (MQ=255)                                                                       cGACGCAACTGTCTACAAGAACGTTCTGGGCTCCTTCCCGTCCGGCGTTAGCGTCATCACCACCCAGGACGACGACGGCTCGGTCGTCGGCCTGACCGCCAGCGCCTTCTCCTCCCTGTCGATGGACCCGCCGCTGGTGCTg                                                                                 <  4:311513/142‑1 (MQ=255)                                                                       cGACGCAACTGTCTACAAGAACGTTCTGGGCTCCTTCCCGTCCGGCGTTACCGTCATCACCACCCAGGACGACGACGGCTCGGTCGTCGGCCTGACCGCCAGCGCCTTCTCCTCCCTGTCGATGGACCCGCCGCTGGTGCTg                                                                                 >  3:311513/1‑142 (MQ=255)                                                                       cGACGCAACTGTCTACAAGAACGTTCTGGGCTCCTTCCCGTCCGGCGTTACCGTCATCACCACCCAGGACGACGACGGCTCGGTCGTCGGCCTGACCGCCAGCGCCTTCTCCTCCCTGTCGATGGACCCGCCGCTGGTGCTGTTCTGcc                                                                          >  4:188038/1‑149 (MQ=255)                                                                           gCAACTGTCTACAAGAACGTTCTGGGCTCCTTCCCGTCCGGCGTTACCGTCATCACCACCCAGGACGACGACGGCTCGGTCGTCGGCCTGACCGCCAGCGCCTTCTCCTCCCTGTCGATGGACCCGCCGCTGGTGCTGTTCTGCCCCaa                                                                      >  4:243898/1‑149 (MQ=255)                                                                                tGTCTACAAGAACGTTCTGGGCTCCTTCCCGTCCGGCGTTACCGTCATCACCACCCAGGACGACGACGGCTCGGTCGTCGGCCTGACCGCCAGCGCCTTCTCCTCCCTGTCGATGGACCCGCCGCTGGTGCTGTTCTGCCCCAACTaca                                                                 >  6:343953/1‑149 (MQ=255)                                                                                  tCTACAAGAACGTTCTGGGCTCCTTCCCGTCCGGCGTTACCGTCATCACCACCCAGGACGACGACGGCTCGGTCGTCGGCCTGACCGCCAGCGCCTTCTCCTCCCTGTCGATGGACCCGCCGCTGGTGCTGTTCTGCCCCAACTacac                                                                >  2:29155/1‑148 (MQ=255)                                                                                      cAAGAACGTTCTGGGCTCCTTCCCGTCCGGCGTTACCGTCATCACCACCCAGGACGACGACGGCTCGGTCGTCGGCCTGACCGCCAGCGCCTTCTCCTCCCTGTCGATGGACCCGCCGCTGGTGCTGTTCTGCCCCAACTACACCTc                                                             <  6:92552/147‑1 (MQ=255)                                                                                       aaGAACGTTCTGGGCTCCTTCCCGTCCGGCGTTACCGTCATCACCACCCAGGACGACGACGGCTCGGTCGTCGGCCTGACCGCCAGCGCCTTCTCCTCCCTGTCGATGGACCCGCCGCTg                                                                                       >  5:252289/1‑120 (MQ=255)                                                                                       aaGAACGTTCTGGGCTCCTTCCCGTCCGGCGTTACCGTCATCACCACCCAGGACGACGACGGCTCGGTCGTCGGCCTGACCGCCAGCGCCTTCTCCTCCCTGTCGATGGACCCGCCGCTg                                                                                       <  6:252289/120‑1 (MQ=255)                                                                                       aaGAACGTTCTGGGCTCCTTCCCGTCCGGCGTTACCGTCATCACCACCCAGGACGACGACGGCTCGGTCGTCGGCCTGACCGCCAGCGCCTTCTCCTCCCTGTCGATGGACCCGCCGCTGGTGCTGTTCTGCCCCAACTACACCTCCGa                                                          >  2:44216/1‑149 (MQ=255)                                                                                       aaGAACGTTCTGGGCTCCTTCCCGTCCGGCGTTACCGTCATCACCACCCAGGACGACGACGGCTCGGTCGTCGGCCTGACCGCCAGCGCCTTATCCTCCCTGTCGATGGACCCGCCGCTGGTGCTGTTCTGCCCCAACTACACCTCCGa                                                          >  4:101214/1‑149 (MQ=255)                                                                                         gAACGTTCTGGGCTCCTTCCCGTCCGGCGTTACCGTCATCACCACCCAGGACGACGACGGCTCGGTCGTCGGCCTGACCGCCAGCGCCTTCTCCTCCCTGTCGATg                                                                                                   <  4:110764/106‑1 (MQ=255)                                                                                                    gCTCCTTCCCGTCCGGCGTTACCGTCATCACCACCCAGGACGACGACGGCTCGGTCGTCGGCCTGACCGCCAGCGCCTTCTCCTCCCTGTCGATGGACCCGCCGCTGGTGCTGTTCTGCCCCAACTACACCTCCGACTCCTACCCGGTa                                             <  3:243898/149‑1 (MQ=255)                                                                                                        cTTCCCGTCCGGCGTTACCGTCATCACCACCCAGGACGACGACGGCTCGGTCGTCGGCCTGACCGCCAGCGCCTTCTCCTCCCTGTCGATGGACCCGCCGCTGGTGCTGTTCTGCCCCAACTACACCTCCGACTCCTACCCGGTACTGa                                         >  4:165701/1‑149 (MQ=255)                                                                                                          tCCCGTCCGGCGTTACCGTCATCACCACCCAGGACGATGACGGCTCGGTCGTCGGCCTGACCGCCAGCGCCTTCTCCTCCCTGTCGATGGACCCGCCGCTGGTGCTGTTCTGCCCCAACTACACCTCCGACTCCTACCCGGTACTGAt                                        >  3:107420/1‑148 (MQ=255)                                                                                                           cccGTCCGGCGTTACCGTCATCACCACCCAGGACGACGACGGCTCGGTCGTCGGCCTGACCGCCAGCGCCTTCTCCTCCCTGTCGATGGACCCGCCGCTGGTGCTGTTCTGCCCCAACTACACCTCCGACTCCTACCCGGTACTGATc                                       <  6:153701/148‑1 (MQ=255)                                                                                                            ccGTCCGGCGTTACCGTCATCACCACCCAGGACGACGACGGCTCGGTCGTCGGCCTGACCGCCAGCGCCTTCTCCTCCCTGTCGATGGACCCGCCGCTGGTGCTGTTCTGCCCCAACTACACCTCCGACTCCTACCCGGTACTGATCaa                                     <  5:343953/149‑1 (MQ=255)                                                                                                                            tcatcaCCACCCAGGACGACGACGGCTCGGTCGTCGGCCTGACCGCCAGCGCCTTCTCCTCCCTGTCGATGGACCCGCCGCTGGTGCTGTTCTGCCCCAACTACACCTCCGACTCCTACCCGGTACTGATCAAGCAGAAGCGCTTTGc                      >  5:108707/1‑148 (MQ=255)                                                                                                                               tcaCCACCCAGGACGACGACGGCTCGGTCGTCGGCCTGACCGCCAGCGCCTTCTCCTCCCTGTCGATGGACCCGCCGCTGGTGCTGTTCTGCCCCAACTACACCTCCGACTCCTACCCGGTACTGATCAAGCAGAAGCGCTTTGCCAtt                  <  1:44216/149‑1 (MQ=255)                                                                                                                                      ccAGGACGACGACGGCTCGGTCGTCGGCCTGACCGCCAGCGCCTTCTCCTCCCTGTCGATGGACCCGCCGCTGGTGCTGTTCTGCCCCAACTACACCTCCGACTCCTACCCGGTACTGATCAAGCAGAAGCGCTTTGCCATTCACctgc           >  3:308830/1‑149 (MQ=255)                                                                                                                                          gccgacgaccGAGCCGTCGTCGTCCTGACCGCCAGCGCCTTCTCCTCCCTGTCGATGGACCCGCCGCTGGTGCTGTTCTGCCCCAACTACACCTCCGACTCCTACCCGGTACTGATCAAGCAGAAGCGCTTTGCCATTCACCTGCTGTc       >  6:141101/3‑149 (MQ=37)                                                                                                                                          gccgacgaccGAGCCGTCGTCGGCCTGACCGCCAGCGCCTTCTCCTCCCTGTCGATGGACCCGCCGCTGGTGCTGTTCTGCCCCAACTACACCTCCGACTCCTACCCGGTACTGATCAAGCAGAAGCGCTTTGCCATTCAc               >  2:34845/3‑141 (MQ=255)                                                                                                                                          gccgacgaccGAGCCGTCGTCGGCCTGACCGCCAGCGCCTTCTCCTCCCTGTCGATGGACCCGCCGCTGGTGCTGTTCTGCCCCAACTACACCTCCGACTCCTACCCGGTACTGATCAAGCAGAAGCGCTTTGCCATTCAc               <  1:34845/139‑1 (MQ=255)                                                                                                                                                gacgGCTCGGTCGTCGGCCTGACCGCCAGCGCCTTCTCCTCCCTGTCGATGGACCCGCCGCTGGTGCTGTTCTGCCCCAACTACACCTCCGACTCCTACCCGGTACTGATCAAGCAGAAGCGCTTTGCCATTCACCTGCTGTCCGGTg  >  4:198535/1‑148 (MQ=255)                                                                                                                                                     |                                                                                                                                               GTATACCATCAGACACATCAACGATAAAAACCGCGGACCACCGCAGCCCTTCAGAGGTACTCCCGATGATCGACGCAACTGTCTACAAGAACGTTCTGGGCTCCTTCCCGTCCGGCGTTACCGTCATCACCACCCAGGACGACGACGGCTCGGTCGTCGGCCTGACCGCCAGCGCCTTCTCCTCCCTGTCGATGGACCCGCCGCTGGTGCTGTTCTGCCCCAACTACACCTCCGACTCCTACCCGGTACTGATCAAGCAGAAGCGCTTTGCCATTCACCTGCTGTCCGGTG  >  NC_002947/4138437‑4138727

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑