Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,138,588 G→C 9.5% S29S (TCG→TCC) ‡ PP_3643 → oxidoreductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,138,5880GC 9.5% 127.6 / 4.0 42S29S (TCG→TCC) ‡PP_3643oxidoreductase
Reads supporting (aligned to +/- strand):  ref base G (20/18);  new base C (2/2);  total (22/20)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.80e-01

ACCATCAGACACATCAACGATAAAAACCGCGGACCACCGCAGCCCTTCAGAGGTACTCCCGATGATCGACGCAACTGTCTACAAGAACGTTCTGGGCTCCTTCCCGTCCGGCGTTACCGTCATCACCACCCAGGACGACGACGGCTCGGTCGTCGGCCTGACCGCCAGCGCCTTCTCCTCCCTGTCGATGGACCCGCCGCTGGTGCTGTTCTGCCCCAACTACACCTCCGACTCCTACCCGGTACTGATCAAGCAGAAGCGCTTTGCCATTCACCTGCTGTCCGGTGAACAGCAGG  >  NC_002947/4138441‑4138736
                                                                                                                                                   |                                                                                                                                                    
aCCATCAGACACATCAACGATAAAAACCGCGGACCACCGCAGCCCTTCAGAGGTACTCCCGATGATCGACGCAACTGTCTACAAGAACGTTCTGGGCTCCTTCCCGTCCGGCGTTACCGTCATCACCACCCAGGACGACGACGGCTCgg                                                                                                                                                     >  4:159824/1‑149 (MQ=255)
        acacATCAACGATAAAAACCGCGGACCACCGCAGCCCTTCAGAGGTACTCCCGATGATCGACGCAACTGTCTACAAGAACGTTCTGGGCTCCTTCCCGTCCGGCGTTACCGTCATCACCACCCAGGACGACGACGGCTCGGTCGTCGGc                                                                                                                                             <  6:134589/149‑1 (MQ=255)
        acacATCAACGATAAAAACCGCGGACCACCGCAGCCCTTCAGAGGTACTCCCGATGATCGACGCAACTGTCTACAAGAACGTTCTGGGCTCCTTCCCGTCCGGCGTTACCGTCATCACCACCCAGGACGACGACGGCTCGGTCGTCGGc                                                                                                                                             <  3:68967/149‑1 (MQ=255)
            aTCAACGATAAAAACCGCGGACCACCGCAGCCCTTCAGAGGTACTCCCGATGATCGACGCAACTGTCTACAAGAACGTTCTGGGCTCCTTCCCGTCCGGCGTTACCGTCATCACCACCCAGGCCGACGACCGAGCCGTCGTCGTCCTgg                                                                                                                                         <  3:313149/149‑2 (MQ=255)
            aTCAACGATAAAAACCGCGGACCACCGCAGCCCTTCAGAGGTACTCCCGATGATCGACGCAACTGTCTACAAGAACGTTCTGGGCTCCTTCCCGTCCGGCGTTACCGTCATCACCACCCAGGACGACGACGGCTCGGTCGTCGGCCTGa                                                                                                                                         >  8:146207/1‑149 (MQ=255)
            aTCAACGATAAAAACCGCGGACCACCGCAGCCCTTCAGAGGTACTCCCGATGATCGACGCAACTGTCTACAAGAACGTTCTGGGCTCCTTCCCGTCCGGCGTTACCGTCATCACCACCCAGGACGACGACGGCTCGGTCGTCGGCCTGa                                                                                                                                         <  1:148598/149‑1 (MQ=255)
             tCAACGATAAAAACCGCGGACCACCGCAGCCCTTCAGAGGTACTCCCGATGATCGACGCAACTGTCTACAAGAACGTTCTGGGCTCCTTCCCGTCCGGCGTTACCGTCATCACCACCCAGGACGACGACGGCTCGGTCGTCGGCCTGAc                                                                                                                                        >  6:281058/1‑149 (MQ=255)
               aaCGATAAAAACCGCGGACCACCGCAGCCCTTCAGAGGTACTCCCGATGATCGACGCAACTGTCTACAAGAACGTTCTGGGCTCCTTCCCGTCCGGCGTTACCGATCTCACCACCCAGGACGACGACGGCTCGGTCGTCGGCCTGACCg                                                                                                                                      >  1:115043/1‑149 (MQ=255)
                 cGATAAAAACCGCGGACCACCGCAGCCCTTCAGGGGTACTCCCGATGATCGACGCAACTGTCTACAAGAACGTTCTGGGCTCCTTCCCGTCCGGCGTTACCGTCATCACCACCCAGGACGACGACGGCTCGGTCGTCGGCCTGACCGcc                                                                                                                                    <  2:104460/149‑1 (MQ=255)
                    tAAAAACCGCGGACCACCGCAGCCCTTCAGAGGTACTCCCGATGATCGACGCAACTGTCTACAAGAACGTTCTGGGCTCCTTCCCGTCCGGCGTTACCGTCATCACCACCCAGGACGACGACGGCTCGGTCGTCGGCCTGa                                                                                                                                         <  6:75867/141‑1 (MQ=255)
                    tAAAAACCGCGGACCACCGCAGCCCTTCAGAGGTACTCCCGATGATCGACGCAACTGTCTACAAGAACGTTCTGGGCTCCTTCCCGTCCGGCGTTACCGTCATCACCACCCAGGACGACGACGGCTCGGTCGTCGGCCTGa                                                                                                                                         >  5:75867/1‑141 (MQ=255)
                    tAAAAACCGCGGACCACCGCAGCCCTTCAGAGGTACTCCCGATGATCGACGCAACTGTCTACAAGAACGTTCTGGGCTCCTTCCCGTCCGGCGTTACCGTCATCACCACCCAGGACGACGACGGCTCGGTCGTCGGCCTGACCGCCAgc                                                                                                                                 <  7:251486/149‑1 (MQ=255)
                         aCCGCGGACCACCGCAGCCCTTCAGAGGTACTCCCGATGATCGACGCAACTGTCTACAAGAACGTTCTGGGCTCCTTCCCGTCCGGCGTTACCGTCATCACCACCCAGGACGACGACGGCTCGGTCGTCGGCCTGACCGCCAGCGCCtt                                                                                                                            <  7:146207/149‑1 (MQ=255)
                            gcgGACCACCGCAGCCCTTCAGAGGTACTCCCGATGATCGACGCAACTGTCTACAAGAACGTTCTGGGCTCCTTCCCGTCCGGCGTTACCGTCATCACCACCCAGGACGACGACGGCTCGGTCGTCGGCCTGACCGCCAGCGCCTtctc                                                                                                                         <  5:281058/149‑1 (MQ=255)
                               gACCACCGCAGCCCTTCAGAGGTACTCCCGATGATCGACGCAACTGTCTACAAGAACGTTCTGGGCTCCTTCCCGTCCGGCGTTACCGTCATCACCCCCCAGGACGACGACGGCTCGGTCGTCGGCCTGCCCGCCAGCGCCTTctcctc                                                                                                                      >  6:23659/1‑149 (MQ=255)
                                    ccGCAGCCCTTCAGAGGTACTCCCGATGATCGACGCAACTGTCTACAAGAACGTTCTGGGCTCCTTCCCGTCCGGCGTTACCGTCATCACCACCCAGGACGACGACGGCTCGGTCGTCGGCCTGACCGCCAGCGCCTTCTCCTCCCTGt                                                                                                                 >  5:153701/1‑149 (MQ=255)
                                        aGCCCTTCAGAGGTACTCCCGATGATCGACGCAACTGTCTACAAGAACGTTCTGGGCTCCTTCCCGTCCGGCGTTACCGTCATCACCACCCAGGACGACGACGGCTCGGTCGTCGGCCTGACCGCCAGCGCCTTCTCCTCCCTGTCGAt                                                                                                             <  2:54710/149‑1 (MQ=255)
                                                        tCCCGATGATCGACGCAACTGTCTACAAGAACGTTCTGGGCTCCTTCCCGTCCGGCGTTACCGTCATCACCACCCAGGACGACGACGGCTCGGTCGTCGGCCTGACCGCCAGCGCCTTCTCCTccc                                                                                                                    >  1:108897/1‑126 (MQ=255)
                                                        tCCCGATGATCGACGCAACTGTCTACAAGAACGTTCTGGGCTCCTTCCCGTCCGGCGTTACCGTCATCACCACCCAGGACGACGACGGCTCGGTCGTCGGCCTGACCGCCAGCGCCTTCTCCTccc                                                                                                                    <  2:108897/126‑1 (MQ=255)
                                                                  cGACGCAACTGTCTACAAGAACGTTCTGGGCTCCTTCCCGTCCGGCGTTAGCGTCATCACCACCCAGGACGACGACGGCTCGGTCGTCGGCCTGACCGCCAGCGCCTTCTCCTCCCTGTCGATGGACCCGCCGCTGGTGCTg                                                                                          <  4:311513/142‑1 (MQ=255)
                                                                  cGACGCAACTGTCTACAAGAACGTTCTGGGCTCCTTCCCGTCCGGCGTTACCGTCATCACCACCCAGGACGACGACGGCTCGGTCGTCGGCCTGACCGCCAGCGCCTTCTCCTCCCTGTCGATGGACCCGCCGCTGGTGCTg                                                                                          >  3:311513/1‑142 (MQ=255)
                                                                  cGACGCAACTGTCTACAAGAACGTTCTGGGCTCCTTCCCGTCCGGCGTTACCGTCATCACCACCCAGGACGACGACGGCTCGGTCGTCGGCCTGACCGCCAGCGCCTTCTCCTCCCTGTCGATGGACCCGCCGCTGGTGCTGTTCTGcc                                                                                   >  4:188038/1‑149 (MQ=255)
                                                                      gCAACTGTCTACAAGAACGTTCTGGGCTCCTTCCCGTCCGGCGTTACCGTCATCACCACCCAGGACGACGACGGCTCGGTCGTCGGCCTGACCGCCAGCGCCTTCTCCTCCCTGTCGATGGACCCGCCGCTGGTGCTGTTCTGCCCCaa                                                                               >  4:243898/1‑149 (MQ=255)
                                                                           tGTCTACAAGAACGTTCTGGGCTCCTTCCCGTCCGGCGTTACCGTCATCACCACCCAGGACGACGACGGCTCGGTCGTCGGCCTGACCGCCAGCGCCTTCTCCTCCCTGTCGATGGACCCGCCGCTGGTGCTGTTCTGCCCCAACTaca                                                                          >  6:343953/1‑149 (MQ=255)
                                                                             tCTACAAGAACGTTCTGGGCTCCTTCCCGTCCGGCGTTACCGTCATCACCACCCAGGACGACGACGGCTCGGTCGTCGGCCTGACCGCCAGCGCCTTCTCCTCCCTGTCGATGGACCCGCCGCTGGTGCTGTTCTGCCCCAACTacac                                                                         >  2:29155/1‑148 (MQ=255)
                                                                                 cAAGAACGTTCTGGGCTCCTTCCCGTCCGGCGTTACCGTCATCACCACCCAGGACGACGACGGCTCGGTCGTCGGCCTGACCGCCAGCGCCTTCTCCTCCCTGTCGATGGACCCGCCGCTGGTGCTGTTCTGCCCCAACTACACCTc                                                                      <  6:92552/147‑1 (MQ=255)
                                                                                  aaGAACGTTCTGGGCTCCTTCCCGTCCGGCGTTACCGTCATCACCACCCAGGACGACGACGGCTCGGTCGTCGGCCTGACCGCCAGCGCCTTCTCCTCCCTGTCGATGGACCCGCCGCTg                                                                                                <  6:252289/120‑1 (MQ=255)
                                                                                  aaGAACGTTCTGGGCTCCTTCCCGTCCGGCGTTACCGTCATCACCACCCAGGACGACGACGGCTCGGTCGTCGGCCTGACCGCCAGCGCCTTCTCCTCCCTGTCGATGGACCCGCCGCTg                                                                                                >  5:252289/1‑120 (MQ=255)
                                                                                  aaGAACGTTCTGGGCTCCTTCCCGTCCGGCGTTACCGTCATCACCACCCAGGACGACGACGGCTCGGTCGTCGGCCTGACCGCCAGCGCCTTCTCCTCCCTGTCGATGGACCCGCCGCTGGTGCTGTTCTGCCCCAACTACACCTCCGa                                                                   >  2:44216/1‑149 (MQ=255)
                                                                                  aaGAACGTTCTGGGCTCCTTCCCGTCCGGCGTTACCGTCATCACCACCCAGGACGACGACGGCTCGGTCGTCGGCCTGACCGCCAGCGCCTTATCCTCCCTGTCGATGGACCCGCCGCTGGTGCTGTTCTGCCCCAACTACACCTCCGa                                                                   >  4:101214/1‑149 (MQ=255)
                                                                                    gAACGTTCTGGGCTCCTTCCCGTCCGGCGTTACCGTCATCACCACCCAGGACGACGACGGCTCGGTCGTCGGCCTGACCGCCAGCGCCTTCTCCTCCCTGTCGATg                                                                                                            <  4:110764/106‑1 (MQ=255)
                                                                                               gCTCCTTCCCGTCCGGCGTTACCGTCATCACCACCCAGGACGACGACGGCTCGGTCGTCGGCCTGACCGCCAGCGCCTTCTCCTCCCTGTCGATGGACCCGCCGCTGGTGCTGTTCTGCCCCAACTACACCTCCGACTCCTACCCGGTa                                                      <  3:243898/149‑1 (MQ=255)
                                                                                                   cTTCCCGTCCGGCGTTACCGTCATCACCACCCAGGACGACGACGGCTCGGTCGTCGGCCTGACCGCCAGCGCCTTCTCCTCCCTGTCGATGGACCCGCCGCTGGTGCTGTTCTGCCCCAACTACACCTCCGACTCCTACCCGGTACTGa                                                  >  4:165701/1‑149 (MQ=255)
                                                                                                     tCCCGTCCGGCGTTACCGTCATCACCACCCAGGACGATGACGGCTCGGTCGTCGGCCTGACCGCCAGCGCCTTCTCCTCCCTGTCGATGGACCCGCCGCTGGTGCTGTTCTGCCCCAACTACACCTCCGACTCCTACCCGGTACTGAt                                                 >  3:107420/1‑148 (MQ=255)
                                                                                                      cccGTCCGGCGTTACCGTCATCACCACCCAGGACGACGACGGCTCGGTCGTCGGCCTGACCGCCAGCGCCTTCTCCTCCCTGTCGATGGACCCGCCGCTGGTGCTGTTCTGCCCCAACTACACCTCCGACTCCTACCCGGTACTGATc                                                <  6:153701/148‑1 (MQ=255)
                                                                                                       ccGTCCGGCGTTACCGTCATCACCACCCAGGACGACGACGGCTCGGTCGTCGGCCTGACCGCCAGCGCCTTCTCCTCCCTGTCGATGGACCCGCCGCTGGTGCTGTTCTGCCCCAACTACACCTCCGACTCCTACCCGGTACTGATCaa                                              <  5:343953/149‑1 (MQ=255)
                                                                                                                       tcatcaCCACCCAGGACGACGACGGCTCGGTCGTCGGCCTGACCGCCAGCGCCTTCTCCTCCCTGTCGATGGACCCGCCGCTGGTGCTGTTCTGCCCCAACTACACCTCCGACTCCTACCCGGTACTGATCAAGCAGAAGCGCTTTGc                               >  5:108707/1‑148 (MQ=255)
                                                                                                                          tcaCCACCCAGGACGACGACGGCTCGGTCGTCGGCCTGACCGCCAGCGCCTTCTCCTCCCTGTCGATGGACCCGCCGCTGGTGCTGTTCTGCCCCAACTACACCTCCGACTCCTACCCGGTACTGATCAAGCAGAAGCGCTTTGCCAtt                           <  1:44216/149‑1 (MQ=255)
                                                                                                                                 ccAGGACGACGACGGCTCGGTCGTCGGCCTGACCGCCAGCGCCTTCTCCTCCCTGTCGATGGACCCGCCGCTGGTGCTGTTCTGCCCCAACTACACCTCCGACTCCTACCCGGTACTGATCAAGCAGAAGCGCTTTGCCATTCACctgc                    >  3:308830/1‑149 (MQ=255)
                                                                                                                                     gccgacgaccGAGCCGTCGTCGTCCTGACCGCCAGCGCCTTCTCCTCCCTGTCGATGGACCCGCCGCTGGTGCTGTTCTGCCCCAACTACACCTCCGACTCCTACCCGGTACTGATCAAGCAGAAGCGCTTTGCCATTCACCTGCTGTc                >  6:141101/3‑149 (MQ=37)
                                                                                                                                     gccgacgaccGAGCCGTCGTCGGCCTGACCGCCAGCGCCTTCTCCTCCCTGTCGATGGACCCGCCGCTGGTGCTGTTCTGCCCCAACTACACCTCCGACTCCTACCCGGTACTGATCAAGCAGAAGCGCTTTGCCATTCAc                        <  1:34845/139‑1 (MQ=255)
                                                                                                                                     gccgacgaccGAGCCGTCGTCGGCCTGACCGCCAGCGCCTTCTCCTCCCTGTCGATGGACCCGCCGCTGGTGCTGTTCTGCCCCAACTACACCTCCGACTCCTACCCGGTACTGATCAAGCAGAAGCGCTTTGCCATTCAc                        >  2:34845/3‑141 (MQ=255)
                                                                                                                                           gacgGCTCGGTCGTCGGCCTGACCGCCAGCGCCTTCTCCTCCCTGTCGATGGACCCGCCGCTGGTGCTGTTCTGCCCCAACTACACCTCCGACTCCTACCCGGTACTGATCAAGCAGAAGCGCTTTGCCATTCACCTGCTGTCCGGTg           >  4:198535/1‑148 (MQ=255)
                                                                                                                                                   ggTCGTCGGCCTGACCGCCAGCGCCTTCTCCTCCCTGTCGATGGACCCGCCGCTGGTGCTGTTCTGCCCCAACTACACCTCCGACTCCTACCCGGTACTGATCAAGCAGAAGCGCTTTGCCATTCACCTGCTGTCCGGTGAACAGCAgg  <  3:165701/149‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
ACCATCAGACACATCAACGATAAAAACCGCGGACCACCGCAGCCCTTCAGAGGTACTCCCGATGATCGACGCAACTGTCTACAAGAACGTTCTGGGCTCCTTCCCGTCCGGCGTTACCGTCATCACCACCCAGGACGACGACGGCTCGGTCGTCGGCCTGACCGCCAGCGCCTTCTCCTCCCTGTCGATGGACCCGCCGCTGGTGCTGTTCTGCCCCAACTACACCTCCGACTCCTACCCGGTACTGATCAAGCAGAAGCGCTTTGCCATTCACCTGCTGTCCGGTGAACAGCAGG  >  NC_002947/4138441‑4138736

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑