Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 322,133 G→A 7.4% intergenic (+420/+38) aguA → / ← PP_0267 agmatine deiminase/ferric siderophore receptor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947322,1330GA 7.4% 155.7 / 2.4 55intergenic (+420/+38)aguA/PP_0267agmatine deiminase/ferric siderophore receptor
Reads supporting (aligned to +/- strand):  ref base G (21/29);  new base A (2/2);  total (23/32)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.06e-01

CACCATGGAAGCATCAGGGTCAGGACGACCGACCCACGACGTCTGCTTGCCGGGCGATACCCCCAAGGGTTGATCAGCGAAGCAGAGATGCAGGCGCTGATGGCGGTGTGGCACTGATCGATTGCACCTGTACCTGCCTCTTCGCGGGCATGCTCGCGAA‑GAGGCA‑GGTACAGGCAACTTAAAATCAGAAACTGTAGCTACCCGTCACCACAAGGCTACGCGGATCGCCCACCTGGATCTGCGCCGCACTGGTCGCCGAGCTGTAGTAGGTCTTGTCAGCAATGTTGCTCAAT  >  NC_002947/321987‑322279
                                                                                                                                                  |                                                                                                                                                    
cacCATGGAAGCATCAGGGTCAGGACGACCGACCCACGACGTCTGCTTGCCGGGCGATACCCCCAAGGGTTGATCAGCGAAGCAGAGATGCAGGCGCTGATGGCGGTGTGGCACTGATCGATTGCACCTGTACCTGCCTCTTCGCGGGc                                                                                                                                                    <  6:128398/149‑1 (MQ=255)
   cATGGAAGCATCAGGGTCAGGACGACCGACCCACGACGTCTGCTTGCCGGGCGATACCCCCAAGGGTTGATCAGCGAAGCAGAGATGCAGGCGCTGATGGCGGTGTGGCACTGATCGATTGCACCTGTACCTGCCTCTTCGCGGGCATg                                                                                                                                                 >  6:99580/1‑149 (MQ=255)
        aaGCATCAGGGTCAGGACGACCGACCCACGACGTCTGCTTGCCGGGCGATACCCCCAAGGGTTGATCAGCGAAGCAGAGATGCAGGCGCTGATGGCGGTGTGGCACTGATCGATTGCACCTGTACCTGCCTCTTCGCGAGCATGCCcgc                                                                                                                                            >  6:173181/1‑149 (MQ=255)
             tCAGGGTCAGGACGACCGACCCACGACGTCTGCTTGCCGGGCGATACCCCCAAGGGTTGATCAGCGAAGCAGAGATGCAGGCGCTGATGGCGGTGTGGCACTGATCGATTGCACCTGTACCTGCCTCTTCGCGGGCATGCTCGCGaa                                                                                                                                         >  6:75318/1‑147 (MQ=255)
             tCAGGGTCAGGACGACCGACCCACGACGTCTGCTTGCCGGGCGATACCCCCAAGGGTTGATCAGCGAAGCAGAGATGCAGGCGCTGATGGCGGTGTGGCACTGATCGATTGCACCTGTACCTGCCTCTTCGCGAGCATGCCCGCGAa‑ga                                                                                                                                      <  6:250257/149‑1 (MQ=255)
             tCAGGGTCAGGACGACCGACCCACGACGTCTGCTTGCCGGGCGATACCCCCAAGGGTTGATCAGCGAAGCAGAGATGCAGGCGCTGATGGCGGTGTGGCACTGATCGATTGCACCTGTACCTGCCTCTTCGCGAGCATGCCCGCGAa‑ga                                                                                                                                      <  5:173181/149‑1 (MQ=255)
               aGGGTCAGGACGACCGACCCACGACGTCTGCTTGCCGGGCGATACCCCCAAGGGTTGATCAGCGAAGCAGAGATGCAGGCGCTGATGGCGGTGTGGCACTGATAGATTGCACCTGTACCTGCCTCTTCGCGGGCATGCTCGCGAA‑GAgg                                                                                                                                    >  2:260862/1‑149 (MQ=255)
                ggggCAGGACGACCGACCCACGACGGCTGCTTGCCGGGCGATACCCCCAAGGGTTGATCAGCGAAGCAGAGATGCAGGCGCTGATGGCGGTGTGGCACTGATCGATTGCACCTGTACCTGCCTCTTCGCGGGCATGCTCGCGAA‑GAGGc                                                                                                                                   <  2:234273/149‑1 (MQ=255)
                  gTCAGGACGACCGACCCACGACGTCTGCTTGCCGGGCGATACCCCCAAGGGTTGATCAGCGAAGCAGAGATGCAGGCGCTGATGGCGGTGTGGCACTGATCGATTGCACCTGTACCTGCCTCTTCGCGGGCATGCTCGCGAA‑GAGGCA‑g                                                                                                                                <  7:220360/149‑1 (MQ=255)
                   tCAGGACGACCGACCCACGACGTCTGCTTGCCGGGCGATACCCCCAAGGGTTGATCAGCGAAGCAGAGATGCAGGCGCTGATGGCGGTGTGGCACTGATCGATTGCACCTGTACCTGCCTCTTCGCgg                                                                                                                                                      >  1:211419/1‑128 (MQ=255)
                   tCAGGACGACCGACCCACGACGTCTGCTTGCCGGGCGATACCCCCAAGGGTTGATCAGCGAAGCAGAGATGCAGGCGCTGATGGCGGTGTGGCACTGATCGATTGCACCTGTACCTGCCTCTTCGCgg                                                                                                                                                      <  2:211419/128‑1 (MQ=255)
                      ggACGACCGACCCACGACGTCTGCTTGCCGGGCGATACCCCCAAGGGTTGATCAGCGAAGCAGAGATGCAGGCGCTGATGGCGGTGTGGCACTGATCGATTGCACCTGTACCTGCCTCTTCGCGGGCATGCTCGCGAA‑GAGGCA‑GGTAc                                                                                                                            <  5:75318/149‑1 (MQ=255)
                         cgaccgacCCACGACGTCTGCTTGCCGGGCGATACCCCCAAGGGTTGATCAGCGAAGCAGAGATGCAGGCGCTGATGGCGGTGTGGCACTGATCGATTGCACCTGTACCTGCCTCTTCGCGGGCATGCTCGCGAA‑GAGGCA‑GGTACAgg                                                                                                                         <  2:68065/149‑1 (MQ=255)
                          gaccgaccCACGACGTCTGCTTGCCGGGCGATACCCCCAAGGGTTGATCAGCGAAGCAGAGATGCAGGCGCTGATGGCGGTGTGGCACTGATCGATTGCACCTGTACCTGCCTCTTCGCGGGCATGCTCGCGAa‑gag                                                                                                                                     <  1:336711/137‑1 (MQ=255)
                          gaccgaccCACGACGTCTGCTTGCCGGGCGATACCCCCAAGGGTTGATCAGCGAAGCAGAGATGCAGGCGCTGATGGCGGTGTGGCACTGATCGATTGCACCTGTACCTGCCTCTTCGCGGGCATGCTCGCGAa‑gag                                                                                                                                     >  2:336711/1‑137 (MQ=255)
                                 ccACGACGTCTGCTTGCCGGGCGATACCCCCAAGGGTTGATCAGCGAAGCAGAGATGCAGGCGCTGATGGCGGTGTGGCACTGATCGATTGCACCTGTACCTGCCTCTTCGCGGGCATGCTCGCGAA‑GAGGCA‑GGTACAGGCAACTTaa                                                                                                                 <  1:260862/149‑1 (MQ=255)
                                 ccACGACGTCTGCTTGCCGGGCGATACCCCCAAGGGTTGATCAGCGAAGCAGAGATGCAGGCGCTGATGGCGGTGTGGCACTGATCGATTGCACCTGTACCTGCCTCTTCGCGGGCATGCTCGCGAA‑GAGGCA‑GGTACAGGCAACTTa                                                                                                                  >  4:84820/1‑148 (MQ=255)
                                  cACGACGTCTGCTTGCCGGGCGATACCCCCAAGGGTTGATCAGCGAAGCAGAGATGCAGGCGCTGATGGCGGTGTGGCACTGATCGATTGCACCTGTACCTGCCTCTTCGCGGGCATGCTCGCGAA‑GAGGCA‑GGTACAGGCAACTTaaa                                                                                                                <  3:90860/149‑1 (MQ=255)
                                  cACGACGTCTGCTTGCCGGGCGATACCCCCAAGGGTTGATCAGCGAAGCAGAGATGCAGGCGCTGATGGCGGTGTGGCACTGATCGATTGCACCTGTACCTGCCTCTTCGCGGGCATACTCGCGAA‑GAGGCA‑GGTACAGGCAACTTaa                                                                                                                 >  5:315700/1‑148 (MQ=255)
                                  cACGACGTCTGCTTGCCGGGCGATACCCCCAAGGGTTGATCAGCGAAGCAGAGATGCAGGCGCTGATGGCGGTGTGGCACTGATCGATTGCACCTGTACCTCCCTCTTCGCGGGCATGCTCGCGAA‑GAGGCA‑GGTACAGGCAACTTaaa                                                                                                                <  7:147459/149‑1 (MQ=255)
                                   acgacgTCTGCTTGCCGGGCGATACCCCCAAGGGGTGATCAGCGAAGCAGAGATGCAGGCGCTGATGGCGGTGTGGCACTGATCGATTGCACCTGAACCTGCCTCTTCGCGGGCATGCTCGCGAA‑GAGGCA‑GGTACAGGCAACTTaaaa                                                                                                               <  5:213192/149‑1 (MQ=255)
                                         tCTGCTTGCCGGGCGATACCCCCAAGGGTTGATCAGCGAAGCAGAGATGCAGGCGCTGATGGCGGTGTGGCACTGATCGATTGCACCTGTACCTGCCTCTTCGCGGGCATGCTCGCGAA‑GAGGCA‑GGTACAGGCAACTTAAAATCAGa                                                                                                          >  2:25575/1‑148 (MQ=255)
                                             cTTGCCGGGCGATACCCCCAAGGGTTGATCAGCGAAGCAGAGATGCAGGCGCTGATGGCGGTGTGGCACTGATCGATTGCACCTGTACCTGCCTCTTCGCGGGCATGCTCGCGAA‑GAGGCA‑GGTACAGGCAACTTAAAATCAGaaa                                                                                                        <  7:54050/146‑1 (MQ=255)
                                                gCCGGGCGATACGCGCATGGGTTGATCAGCGAAGCAGAGATGCAGGCGCTGTTGGCGGTGTGGCACTGATCGATTGCACCTGTACCTGCCTGTTCGCGGGCATGCTCGCGAA‑GAGGCATGGTACAGGCAACTTAAAATCAGAAACTGt                                                                                                    <  1:25575/148‑1 (MQ=255)
                                                gCCGGGCGATACCCCCAAGGGTTGATCAGCGAAGCAGAGATGCAGGCGCTGATGGCGGTGTGGCACTGATCGATTGCACCTGTACCTGCCTCTTCGCGGGCATGCTCGCGAA‑GAGGCA‑GGTACAGGCAACTTAAAATCAGAAACTGTa                                                                                                   <  3:93779/148‑1 (MQ=255)
                                                    ggCGATACCCCCAAGGGTTGATCAGCGAAGCAGAGATGCAGGCGCTGATGGCGGTGTGGCACTGATCGATTGCACCTGTACCTGCCTCTTCGCGGGCATGCTCGCGAA‑GAGGCA‑GGTACAGGCAACTTAAAATCAGAAACTGTAGCTAc                                                                                              <  3:84820/149‑1 (MQ=255)
                                                         tACCCCCAAGGGTTGATCAGCGAAGCAGAGATGCAGGCGCTGATGGCGGTGTGGCACTGATCGATTGCACCTGTACCTGCCTCTTCGCGGGCATGCTCGCGAA‑GAGGCA‑GGTACAGGCAACTTAAAATCAGAAACTGTAGCTACCCGt                                                                                          >  5:136461/1‑148 (MQ=255)
                                                            ccccAAGGGTTGATCAGCGAAGCAGAGATGCAGGCGCTGATGGCGGTGTGGCACTGATCGATTGCACCTGTACCTGCCTCTTCGCGGGCATGCTCGCGAA‑GAGGCA‑GGTACAGGCAACTTAAAATCAGAAACTGTAGCTACCCGTcac                                                                                       >  4:289292/1‑148 (MQ=255)
                                                            ccccAAGGGTTGATCAGCGAAGCAGAGATGCAGGCGCTGATGGCGGTGTGGCACTGATCGATTGCACCTGTACCTGCCTCTTCGCGGGCATGCTCGCGAA‑GAGGCA‑GGTACAGGCAACTTAAAATCAGAAACTGTAGCTACCCGTcac                                                                                       >  5:337131/1‑148 (MQ=255)
                                                              ccAAGGGTTGATCAGCGAAGCAGAGATGCAGGCGCTGATGGCGGTGTGGCACTGATCGATTGCACCTGTACCTGCCTCTTCGCGGGCATGCTCGCGAA‑GAGGCA‑GGTACAGGCAACTTAAAATCAGAAACTGTAGCTACCCGTcacca                                                                                     >  8:270334/1‑148 (MQ=255)
                                                                aaGGGTTGATCAGCGAAGCAGAGATGCAGGCGCTGATGGCGGTGTGGCACTGATCGATTGCACCTGTACCTGCCTCTTCGCGGGCATACTCGCGAA‑GAGGCA‑GGTACAGGCAACTTAAAATCAGAAACTGTAGCTACCCGTCACCACaa                                                                                  <  6:315700/149‑1 (MQ=255)
                                                                       gATCAGCGAAGCAGAGATGCAGGCGCTGATGGCGGTGTGGCACTGATCGATTGCACCTGTACCTGCCTCTTCGCGGGCATGCTCGCGAA‑GAGGCA‑GGTACAGGCAACTTAAAATCAGAAACTGTAGCTACCCGTCACCACAAGGCTAcg                                                                           >  4:137586/1‑149 (MQ=255)
                                                                                   agagATGCAGGCGCTTATGGCGGTGTGGCACTGATCGATTGCACCTGTACCTGCCTCTTCGCGGGCATGCTCGCGAA‑GAGGCA‑GGTACAGGCAACTTAAAATCAGAAACTGTAGCTACCCGTCACCACAAGGCTACGCGGATCGCCCAc                                                               <  2:277880/149‑1 (MQ=255)
                                                                                   agagATGCAGGCGCTGATGGCGGTGTGGCACTGATCGATTGCACCTGTACCTGCCTCTTCGCGGGCATGCTCGCGAA‑GAGGCA‑GGTACAGGCAACTTAAAATCAGAAACTGTAGCTACCCGTCACCACAAGGCTACGCGGATCGCCCAc                                                               <  3:289292/149‑1 (MQ=255)
                                                                                   agagATGCAGGCGCTGATGGCGGTGTGGCACTGATCGATTGCACCTGTACCTGCCTCTTCGCGGGCATGCTCGCGAA‑GAGGCA‑GGTACAGGCAACTTAAAATCAGAAACTGTAGCTACCCGTCACCACAAGGCTACGCGGATCGCCCAc                                                               <  2:281708/149‑1 (MQ=255)
                                                                                     agaTGCAGGCGCTGATGGCGGTGTGGCACTGATCGATTGCACCTGTACCTGCCTCTTCGCGGGCATGCTCGCGAA‑GAGGCA‑GGTACAGGCAACTTAAAATCAGAAACTGTAGCTACCCGTCACCACAAGGCTACGCGGATCGCCCACCt                                                             <  7:270334/149‑1 (MQ=255)
                                                                                      gaTGCAGGCGCTGATGGCGGTGTGGCACTGATCGATTGCACCTGTACCTGCCTCTTCGCGGGCATGCTCGCGAA‑GAGGCA‑GGTACAGGCAACTTAAAATCAGAAACTGTAGCTACCCGTCACCACAAGGCTACGCGGATCGCCCACCTg                                                            >  1:149725/1‑149 (MQ=255)
                                                                                         gCAGGCGCTGATGGCGGTGTGGCACTGATCGATTGCACCTGTACCTGCCTCTTCGCGGGCATGCTCGCGAA‑GAGGCA‑GGTACAGGCAACTTAAAATCAGAAACTGTAGCTACCCGTCACCACaa                                                                                  <  7:290869/124‑1 (MQ=255)
                                                                                         gCAGGCGCTGATGGCGGTGTGGCACTGATCGATTGCACCTGTACCTGCCTCTTCGCGGGCATGCTCGCGAA‑GAGGCA‑GGTACAGGCAACTTAAAATCAGAAACTGTAGCTACCCGTCACCACaa                                                                                  >  8:290869/1‑124 (MQ=255)
                                                                                                 tGATGGCGGTGTGGCACTGATCGATTGCACCTGTACCTGCCTCTTCGCGGGCATGCTCGCGAA‑GAGGCA‑GGTACAGGCAACTTaaaa                                                                                                               >  2:154053/1‑87 (MQ=255)
                                                                                                 tGATGGCGGTGTGGCACTGATCGATTGCACCTGTACCTGCCTCTTCGCGGGCATGCTCGCGAA‑GAGGCA‑GGTACAGGCAACTTaaaa                                                                                                               <  1:154053/87‑1 (MQ=255)
                                                                                                 tGATGGCGGTGTGGCACTGATCGATTGCACCTGTACCTGCCTCTTCGCGGGCATGCTCGCGAA‑GAGGCA‑GGTACAGGCAACTTAAAATCAGAAACTGTAGCTACCCGTCACCACAAGGCTACGCGGATCGCCCACCTGGATCTGcgccg                                                 >  6:345839/1‑149 (MQ=255)
                                                                                                      gCGGTGTGGCACTGATCGATTGCACCTGTACCTGCCTCTTCGCGGGCATGCTCGCGAA‑GAGGCA‑GGTACAGGCAACTTAAAATCAGAAACTGTAGCTACCCGTCACCACAAGGCTACGCGGATCGCCCACCTGGATCTGCGCCGCACTg                                            <  2:149725/149‑1 (MQ=255)
                                                                                                               cACTGATCGATTGCACCTGTACCTGCCTCTTCGCGGGCATGCTCGCGAA‑GAGGCA‑GGTACAGGCAACTTAAAATCAGAAACTGTAGCTACCCGTCACCACAAGGCTACGCGGATCGCCCACCTGGATCTGCGCCGCAc                                              <  4:296121/138‑1 (MQ=255)
                                                                                                               cACTGATCGATTGCACCTGTACCTGCCTCTTCGCGGGCATGCTCGCGAA‑GAGGCA‑GGTACAGGCAACTTAAAATCAGAAACTGTAGCTACCCGTCACCACAAGGCTACGCGGATCGCCCACCTGGATCTGCGCCGCAc                                              >  3:296121/1‑138 (MQ=255)
                                                                                                                aCTGATCGATTGCACCTGTACCTGCCTCTTCGCGGGCATGCTCGCGAA‑GAGGCA‑GGTACAGGCAACTTAAAATCAGAAACTGTAGCTACCCGTCACCACAAGGCTACGCGGATCGCCCCCCTGGATCTGCGCCGCACTGGTCGCCGAGc                                  >  3:249185/1‑149 (MQ=255)
                                                                                                                  tGATCGATTGCACCTGTACCTGCCTCTTCGCGTGCATGCTCGCGAA‑GAGGCA‑GGTACAGGCAACTTAAAATCAGAAACTGTAGCTACCCGTCACCACAAGGCTACGCGGATCGCCCACCTGGATCTGcgcc                                                  <  6:256197/131‑1 (MQ=255)
                                                                                                                  tGATCGATTGCACCTGTACCTGCCTCTTCGCGGGCATGCTCGCGAA‑GAGGCA‑GGTACAGGCAACTTAAAATCAGAAACTGTAGCTACCCGTCACCACAAGGCTACGCGGATCGCCCACCTGGATCTGcgcc                                                  >  5:256197/1‑131 (MQ=255)
                                                                                                                    aTCGATTGCACCTGTACCTGCCTCTTCGCGGGCATGCTCGCGAA‑GAGGCA‑GGTACAGGCAACTTAAAATCAGAAACTGTAGCTACCCGTCACCACAAGGCTACGCGGATCGCCCACCTGGATCTGCGCCGCACTGGTCGCCGAGCTg                                <  5:345839/147‑1 (MQ=255)
                                                                                                                            cACCTGTACCTGCCTCTTCGCGGGCATGCTCGCGAA‑GAGGCA‑GGTACAGGCAACTTAAAATCAGAAACTGTAGCTACCCGTCACCACAAGGCTACGCGGATCGCCCACCTGGATCTGCGCCGCACTGGTCGCCGAGCTgtagta                           <  8:68675/144‑1 (MQ=255)
                                                                                                                            cACCTGTACCTGCCTCTTCGCGGGCATGCTCGCGAA‑GAGGCA‑GGTACAGGCAACTTAAAATCAGAAACTGTAGCTACCCGTCACCACAAGGCTACGCGGATCGCCCACCTGGATCTGCGCCGCACTGGTCGCCGAGCTGtagtagagat                      >  7:68675/1‑145 (MQ=255)
                                                                                                                            cACCTGTACCTGCCTCTTCGCGGGCATGCTCGCGAA‑GAGGCA‑GGTACAGGCAACTTAAAATCAGAAACTGTAGCTACCCGTCACCACAAGGCTACGCGGATCGCCCACCTGGATCTGCGCCGCACTGGTCGCCGAGCTGTAGTAGGTc                       <  6:136461/148‑1 (MQ=255)
                                                                                                                                  tACCTGCCTCTTCGCGGGCATGCTCGCGAAGGAGGCA‑GGTACAGGCAACTTAAAATCAGAAACTGTAGCTACCCGTCACCACAAGGCTACGCGGATCGCCCACCTGGATCTGCGCCGCACTGGTCGCCGAGCTGTAGTAGGtgttgt                   >  1:30927/1‑147 (MQ=255)
                                                                                                                                  tACCTGCCTCTTCGCGGGCATGCTCGCGAA‑GAGGCA‑GGTACAGGCAACTTAAAATCAGAAACTGTAGCTACCCGTCACCACAAGGCTACGCGGATCGCCCACCTGGATCTGCGCCGCACTGGTCGCCGAGCTGTAGTAGGTCTTGTCAg                <  3:137586/149‑1 (MQ=255)
                                                                                                                                          tctTCGCGAGCATGCCCGCGAA‑GAGGCA‑GGTACAGGCAACTTAAAATCAGAAACTGTAGCTACCCGTCACCACAAGGCTACGCGGATCGCCCACCTGGATCTGCGCCGCACTGGTCGCCGAGCTGTAGTAGGTCTTGTCAGCGATGTTg        >  7:58124/1‑149 (MQ=255)
                                                                                                                                            ttCGCGGGCATGCTCGCGAA‑GAGGCA‑GGTACAGGCAACTTAAAATCAGAAACTGTAGCTACCCGTCACCACAAGGCTACGCGGATCGCCCACCTGGATCTGCGCCGCACTGGTCGCCGAGCTGTAGTAGGTCTTGTCAGCAATGTTg        <  2:30927/147‑1 (MQ=255)
                                                                                                                                                cgGGCATGCTCGCGAA‑GAGGCA‑GGTACAGGCAACTTAAAATCAGAAACTGTAGCTACCCGTCACCACAAGGCTACGCGGATCGCCCACCTGGATCTGCGCCGCACTGGTCGCCGAGCTGTAGTAGGTCTTGTCAGCAATGTTGCTCAAt  <  3:65842/149‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                    
CACCATGGAAGCATCAGGGTCAGGACGACCGACCCACGACGTCTGCTTGCCGGGCGATACCCCCAAGGGTTGATCAGCGAAGCAGAGATGCAGGCGCTGATGGCGGTGTGGCACTGATCGATTGCACCTGTACCTGCCTCTTCGCGGGCATGCTCGCGAA‑GAGGCA‑GGTACAGGCAACTTAAAATCAGAAACTGTAGCTACCCGTCACCACAAGGCTACGCGGATCGCCCACCTGGATCTGCGCCGCACTGGTCGCCGAGCTGTAGTAGGTCTTGTCAGCAATGTTGCTCAAT  >  NC_002947/321987‑322279

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑