Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,282,243 G→A 6.2% Y329Y (TAC→TAT)  PP_3753 ← AraC family transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,282,2430GA 6.2% 190.6 / 2.0 64Y329Y (TAC→TAT) PP_3753AraC family transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base G (30/30);  new base A (2/2);  total (32/32)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.77e-01

CCCGCTCCTACAATTCTGGGCGTGCCCTGTAGGAGCGGGTTTACCCGCGAACACCGGCAGAGCCGGTGCCATCCACCGCGGTGGTTT‑CTTCGCGGGCATGCCCGCTCCTACAGGTAGAACGCAGGCCGACCAGCCGCGCCCGGTAGCTCCCTGGGCTCTGCCCGGT‑CCACTTCTTGAACGCCCGGTGGAAGGCACTCGGTTCCTGAAACCCCGCCTGCTCGGCAATTTCGGCAATGCTCAACGCGCCTTCGCGCAGCCGCTCGAAGGCCATGGCCCGGCGCACTTCAT  >  NC_002947/4282101‑4282388
                                                                                                                                               |                                                                                                                                                  
cccGCTCCTACAATTCTGGGCGTGCCCTGTAGGAGCGGGTTTACCCGCGAACACCGGCAGAGCCGGTGCCATCCACCGCGGTGGTTT‑CTTCGCGGGCATGCCCGCTCCTACAGGTAGAACGCAGGCCGACCAGCCGCGCCCGGTAGCTc                                                                                                                                              >  5:137561/1‑149 (MQ=255)
    cTCCTACAATTCTGGGCGTGCCCTGTAGGAGCGGGTTTACCCGCGAACACCGGCAGAGCCGGTGCCATCCACCGCGGTTGTTT‑CTTCGCGGGCATGCCCGCTCCTACAGGTAGAACGCAGGCCGACCAGCCGCGCCCGGTAGCTCCCTg                                                                                                                                          <  4:87094/149‑1 (MQ=255)
       cTACAATTCTGGGCGTGCCCTGTAGGAGCGGGTTTACCCGCGAACACCGGCAGAGCCGGTGCCATCCACCGCGGTGGTTT‑CTTCGCGGGCATGCCCGCTCCTACAGGTAGAACGCAGGCCGACCAGCCGCGCCCGGTAGCTCCCTggg                                                                                                                                        <  5:212069/148‑1 (MQ=255)
       cTACAATTCTGGGCGTGCCCTGTAGGAGCGGGTTTACCCGCGAACACCGGCAGAGCCGGTGCCATCCACCGCGGTGGTTT‑CTTCGCGGGCATGCCCGCTCCTACAGGTAGAACGCAGGCCGACCAGCCGCGCCCGGTAGCTCCCTGGGc                                                                                                                                       <  6:50259/149‑1 (MQ=255)
          cAATTCTGGGCGTGCCCTGTAGGAGCGGGTTTACCCGCGAACACCGGCAGAGCCGGTGCCTTCCACCGCGGTGGTTT‑CTTCGCGGGCATGCCCGCTCCTACAGGTAGAACGCAGGCCGACCAGCCGCGCCCGGTAGCTCCCTGGGctct                                                                                                                                    <  3:143247/149‑1 (MQ=255)
          cAATTCTGGGCGTGCCCTGTAGGAGCGGGTTTACCCGCGAACACCGGCAGAGCCGGTGCCATCCACCGCGGTGGTTT‑CTTCGCGGGCATGCCCGCTCCTACAGGTAGAACGCAGGCCGACCAGCCGCGCCCCGTAGCTCCCTGGGctct                                                                                                                                    >  3:108230/1‑149 (MQ=255)
                         ccTGTAGGAGCGGGTTTACCCGCGAACACCGGCAGAGCCGGTGCCATCCACCGCGGTGGTTT‑CTTCGCGGGCATGCCCGCTCCTACAGGTAGAACGCAGGCCGACCAGCCGCGCCCGGTAGCTCCCTGGGCTCTGCCCGGT‑CCActtct                                                                                                                    >  4:152670/1‑149 (MQ=255)
                          cTGTAGGAGCGGGTTTACCCGCGAACACCGGCAGAGCCGGTGCCATCCACCGCGGTGGTTT‑CTTCGCGGGCATGCCCGCTCCTACAGGTAGAACGCAGGCCGACCAGCCGCGCCCGGTAGCTCCCTGGGCTCTGCCCGGT‑CCActtctt                                                                                                                   <  1:112666/149‑1 (MQ=255)
                          cTGTAGGAGCGGGTTTACCCGCGAACACCGGCAGAGCCGGTGCCATCCACCGCGGTGGTTT‑CTTCGCGGGCATGCCCGCTCCTACAGGTAGAACGCAGGCCGACCAGCCGCGCCCGGTAGCTCCCTGGGCTCTGCCCGGT‑CCActtctt                                                                                                                   >  6:185786/1‑149 (MQ=255)
                          cTGTAGGAGCGGGTTTACCCGCGAACACCGGCAGAGCCGGTGCCATCCACCGCGGTGGTTT‑CTTCGCGGGCATGCCCGCTCCTACAGGTAGAACGCAGGCCCACCAGCCGCGCCCGGTAGCTCCCTGGGCTCTGCCCGGT‑CCActtctt                                                                                                                   <  1:245427/149‑1 (MQ=255)
                             tAGGAGCGGGTTTACCCGCGAACACCGGCAGAGCCGGTGCCATCCACCGCGGTGGTTT‑CTTCGCGGGCATGCCCGCTCCTACAGGTAGAACGCAGGCCGACCAGCCGCGCCCGGTAGCTCCCTGGGCTCTGCCCGGT‑CCAGTTCTTGaa                                                                                                                >  8:220433/1‑149 (MQ=255)
                             tAGGAGCGGGTTTACCCGCGAACACCGGCAGAGCCGGTGCCATCCACCGCGGTGGTTT‑CTTCGCGGGCATGCCCGCTCCTACAGGTAGAACGCAGGCCGACCAGCCGCGCCCGGTAGCTCCCTGGGCTCTGCCCGGT‑CCACTTCTTGaa                                                                                                                >  6:341089/1‑149 (MQ=255)
                             tAGGAGCGGGTTTACCCGCGAACACCGGCAGAGCCGGTGCCATCCACCGCGGTGGTTT‑CTTCGCGGGCATGCCCGCTCCTACAGGTAGAACGCAGGCCGACCAGCCGCGCCCGGTAGCTCCCTGGGCTCTGCCCGGT‑CCACTTCTTGaa                                                                                                                <  8:12763/149‑1 (MQ=255)
                                gAGCGGGTTTACCCGCGAACACCGGCAGAGCCGGTGCCATCCACCGCGGTGGTTTGCTTCGCGGGCATGCCCGCTCCTACAGGTAGAACGCAGGCCGACCAGCCGCGCCCGGTAGCTCCCTGGGCTCTGCCCGGT‑CCACTTCTTGAACg                                                                                                              >  1:93103/1‑149 (MQ=255)
                                  gCGGGTTTACCCGCGAACACCGGCAGAGCCGGTGCCATCCACCGCGGTGGTTT‑CTTCGCGGGCATGCCCGCTCCTACAGGTAGAACGCAGGCCGACCAGCCGCGCCCGGTAGCTCCCTGGGCTCTGCCCGGT‑CCACTTCTTGAACGccc                                                                                                           >  7:311823/1‑149 (MQ=255)
                                  gCGGGTTTACCCGCGAACACCGGCAGAGCCGGTGCCATCCACCGCGGTGGTTT‑CTTCGCGGGCATGCCCGCTCCTACAGGTAGAACGCAGGCCGACCAGCCGCGCCCGGTAGCTCCCTGGGCTCTGCCCGGT‑CCACTTCTTGAACGccc                                                                                                           <  1:297140/149‑1 (MQ=255)
                                   cGGGTTTACCCGCGAACACCGGCAGAGCCGGTGCCATCCACCGCGGTGGTTT‑CTTCGCGGGCATGCCCGCTCCTACAGGTAGAACGCAGGCCGACCAGCCGCGCCCGGTAGCTCCCTGGGCTCTGCCCGGT‑CCACTTCTTGAACGccc                                                                                                           <  4:170790/148‑1 (MQ=255)
                                      gTTTACCCGCGAACACCGGCAGAGCCGGTGCCATCCACCGCGGTGGTTT‑CTTCGCGGGCATGCCCGCTCCTACAGGTAGAACGCAGGCCGACCAGCCGCGCCCGGTAGCTCCCTGGGCTCTGCCCGGT‑CCACTTCTTGAACGCCCGGTg                                                                                                       <  6:137561/149‑1 (MQ=255)
                                          aCCCGCGAACACCGGCAGAGCCGGTGCCATCCACCGCGGTGGTTT‑CTTCGCGGGCATGCCCGCTCCTACAGGTAGAACGCAGGCCGACCAGCCGCGCCCGGTAGCTCCCTGGGCTCTGCCCGGT‑CCACTTCTTGAACGCCCGGTGGAAg                                                                                                   >  6:329114/1‑149 (MQ=255)
                                          aCCCGCGAACACCGGCAGAGCCGGTGCCATCCACCGCGGGGGTTT‑CTTCGCGGGCATGCCCGCTCCTACAGGTAGAACGCAGGCCGACCAGCCGCGCCCGGTAGCTCCCTGGGCTCTGCCCGGT‑CTACTTCTTGAACGCCCGGTGGaa                                                                                                    <  7:210201/148‑1 (MQ=255)
                                           cccGCGAACACCGGCAGAGCCGGTGCCATCCACCGCGGTGGTTT‑CTTCGCGGGCATGCCCGCTCCTACAGGTAGAACGCAGGCCGACCAGCCGCGCCCGGTAGCTCCCTGGGCTCTGCCCGGT‑CCACTTCTTGAACGCCCGGTGGAAg                                                                                                   <  5:185786/148‑1 (MQ=255)
                                           cccGCGAACACCGGCAGAGCCGGTGCCATCCACCGCGGTGGTTT‑CTTCGCGGGCATGCCCGCTCCTACAGGTAGAACGCAGGCCGACCAGCCGCGCCCGGTAGCTCCCTGGGCTCTGCCCGGT‑CCACTTCTTGAACGCCCGGTGGAAg                                                                                                   <  5:79074/148‑1 (MQ=255)
                                            ccGCGAACACCGGCAGAGCCGGTGCCATCCACCGCGGTGGTTT‑CTTCGCGGGCATGCCCGCTCCTACAGGTAGAACGCAGGCCGACCAGCCGCGCCCGGTAGCTCCCTGGGCTCTGCCCGGT‑CCACTTCTTGAACGCCCGGt                                                                                                        <  3:216520/142‑1 (MQ=255)
                                            ccGCGAACACCGGCAGAGCCGGTGCCATCCACCGCGGTGGTTT‑CTTCGCGGGCATGCCCGCTCCTACAGGTAGAACGCAGGCCGACCAGCCGCGCCCGGTAGCTCCCTGGGCTCTGCCCGGT‑CCACTTCTTGAACGCCCGGt                                                                                                        >  4:216520/1‑142 (MQ=255)
                                                gAACACCGGCAGAGCCGGTGCCATCCACCGCGGTGGTTT‑CTTCGCGGGCATGCCCGCTCCTACAGGTAGAACGCAGGCCGACCAGCCGCGCCCGGTAGCTCCCTGGGCTCTGCCCGGT‑CCACTTCTTGAACGCCCGGTGGAAGGCACTc                                                                                             <  2:201007/149‑1 (MQ=255)
                                                   cacCGGCAGAGCCGGTGCCATCCACCGCGGTGGTTT‑CTTCGCGGGCATGCCCGCTCCTACAGGTAGAACGCAGGCCGACCAGCCGCGCCCGGTAGCTCCCTGGGCTCTGCCCGGT‑CCACTTCTTGAACGCCCGGTGGAAGGCACTCGGt                                                                                          <  4:215545/149‑1 (MQ=255)
                                                     ccGGCAGAGCCGGAGCCATCCACCGCGGTGGATT‑CTTCACGGGCATGCCCGCTACTACAGGTAGAACGCAGGCCGACCAGCCGCGCCCGGTACCTCCCACGGCACTGCCCCGT‑CCACTTCTTGAACGCCCGCTGGAAGGCACTCGGTTc                                                                                        >  2:271793/1‑149 (MQ=38)
                                                       ggCAGAGCCGGTGCCATCCACCGCGGTGGTTT‑CTTCGCGGGCATGCCCGCTCCTACAGGTAGAACGCAGGCCGACCAGCCGCGCCCGGTAGCTCCCTGGGCTCTGCCCGGT‑CCACTTCTTGAACGCCCGGTGGAAGGCACTCGGTTCCt                                                                                      >  7:175002/1‑149 (MQ=255)
                                                       ggCAGAGCCGGTGCCATCCACCGCGGTGGTTT‑CTTCGCGGGCATGCCCGCTCCTACAGGTAGAACGCAGGCCGACCAGCCGCGCCCGGTAGCTCCCTGGGCTCTGCCCGGT‑CCACTTCTTGAACGCCCGGTGGAAGGCACTCGGTTCCt                                                                                      <  3:140867/149‑1 (MQ=255)
                                                        gCAGAGCCGGTGCCATCCACCGCGGTGGTTT‑CTTCGCGGGCATGCCCGCTCCTACAGGTAGAACGCAGGCCGACCAGCCGCGCCCGGTAGCTCCCTGGGCTCTGCCCGGT‑CCACTTCTTGAACGCCCGGTGGAAGGCACTCGGTTCCt                                                                                      >  4:312495/1‑148 (MQ=255)
                                                             gCCGGTGCCATCCACCGCGGTGGTTT‑CTTCGCGGGCATGCCCGCTCCTACAGGTAGAACGCAGGCCGACCAGCCGCGCCCGGTAGCTCCCTGGGCTCTGCCCGGT‑CCACTTCTTGAACGCCCGGTGGAAGGCACTCGGTTCCTGaaa                                                                                  <  6:207615/147‑1 (MQ=255)
                                                             gCCGGTGCCATCCACCGCGGTGGTTT‑CTTCGCGGGCATGCCCGCTCCTACAGGTAGAACGCAGGCCGACCAGCCGCGCCCGGTAGCTCCCTGGGCTCTGCCCGGT‑CCACTTCTTGAACGCCCGGTGGAAGGCACTCGGTTCCTGaaa                                                                                  >  5:207615/1‑147 (MQ=255)
                                                                 gTGCCATCCACCGCGGTGGTTT‑CTTCGCGGGCATGCCCGCTCCTACAGGTAGAACGCAGGCCGACCAGCCGCGCCCGGTAGCTCCCTGGGCTCTGCCCGGT‑CCACTTCTTGAACGCCCGGTGGAAGGCACTCGGTTCCTGAAACCCCGc                                                                            <  6:146838/149‑1 (MQ=255)
                                                                      aTCCACCGCGGTGGTTT‑CTTCGCGGGCATGCCCGCTCCTACAGGTAGAACGCAGGCCGACCAGCCGCGCCCGATAGCTCCCTGGGCTCTGCCCGGT‑CCACTTCTTGAACGCCCGGTGGAAGGCACTCGGTTCCTGAAACCCCGCCTGc                                                                        >  2:278148/1‑148 (MQ=255)
                                                                      aTCCACCGCGGTGGTTT‑CTTCGCGGGCATGCCCGCTCCTACAGGTAGAACGCAGGCCGACCAGCCGCGCCCGATAGCTCCCTGGGCTCTGCCCGGT‑CCACTTCTTGAACGCCCGGTGGAAGGCACTCGGTTCCTGAAACCCCGCCTGCt                                                                       >  2:274078/1‑149 (MQ=255)
                                                                       tCCACCGCGGTGGTTT‑CTTCGCGGGCATGCCCGCTCCTACAGGTAGAACGCAGGCCGACCAGCCGCGCCCGGTAGCTCCCTGGGCTCTGCCCGGT‑CCACTTCTTGAACGCCCGGTGGAAGGCACTCGGTTCCTGAAAc                                                                                 <  6:137464/138‑1 (MQ=255)
                                                                        ccACCGCGGTGGTTT‑CTTCGCGGGCATGCCCGCTCCTACAGGTAGAACGCAGGCCGACCAGCCGCGCCCGGTAGCTCCCTGGGCTCTGCCCGGT‑CCACTTCTTGAACGCCCGGTGGAAGGCACTCGGTTCCTGAAACCCCGCCTGCTCg                                                                     >  7:9364/1‑149 (MQ=255)
                                                                         cACCGCGGTGGTTT‑CTTCGCGGGCATGCCCGCTCCTACAGGTAGAACGCAGGCCGCCCAGCCGCGCCCGGTAGCTCCCTGGGCTCTGCCCGGT‑CCACTTCTTGAACGCCCGGTGGAAGGCACTCGGTTCCTGAAACCCCGCCTGCTCgg                                                                    >  3:326731/1‑149 (MQ=255)
                                                                         cACCGCGGTGGTTT‑CTTCGCGGGCATGCCCGCTCCTACAGGTAGAACGCAGGCCGACCAGCCGCGCCCGGTAGCTCCCTGGGCTCTGCCCGGT‑CCACTTCTTGAACGCCCGGTGGAAGGCACTCGGTTCCTGAAACCCCGCCTGCTCgg                                                                    >  8:265827/1‑149 (MQ=255)
                                                                                 tggtTT‑CTTCGCGGGCATGCCCGCTCCTACAGGTAGAACGCAGGCCGACCAGCCGCGCCCGGTAGCTCCCTGGGCTCTGCCCGGT‑CCACTTCTTGAACGCCCGGTGGAAGGCACTCGGTTCCTGAAACCCCGCCTGCTCGGCAATTTCg                                                            <  5:329114/149‑1 (MQ=255)
                                                                                 tggtTT‑CTTCGCGGGCATGCCCGCTCCTACAGGTAGAACGCAGGCCGACCAGCCGCGCCCGGTAGCTCCCTGGGCTCTGCCCGGT‑CCACTTCTTGAACGCCCGGTGGAAGGCACTCGGTTCCTGAAACCCCGCCTGCTCGGCAATTTCg                                                            <  3:152670/149‑1 (MQ=255)
                                                                                   gtTT‑CTTCGCGGGCATGCCCGCTCCTACAGGTAGAACGCAGGCCGACCAGCCGCGCCCGGTAGCTCCCTGGGCTCTGCCCGGT‑CCACTTCTTGAACGCCCGGTGGAAGGCACTCGGTTCCTGAAACCCCGCCTGCTCGGCAATTTCgg                                                           >  5:52041/1‑148 (MQ=255)
                                                                                   gtTT‑CTTCGCGGGCATGCCCGCTCCTACAGGTAGAACGCAGGCCGACCAGCCGCGCCCGGTAGCTCCCTGGGCTCTGCCCGGT‑CCACTTCTTGAACGCCCGGTGGAAGGCACCCGGTTCCTGAAACCCCGCCTGCTCGGCAATTTCgg                                                           <  6:99273/148‑1 (MQ=255)
                                                                                    ttt‑CTTCGCGGGCATGCCCGCTCCTACAGGAAGAACGCAGGCCGACCAGCCGCGCCCGGTAGCTCCCTGGGCTCTGCCCGGT‑CCACTTCTTGAACGCCCGGTGGAAGGCACTCGGTTCCTGAAACCCCGCCTGCTCGGCAATTTCGGc                                                          >  4:242797/1‑148 (MQ=255)
                                                                                        cttcGCGGGCATGCCCGCTCCTACAGGTAGAACGCAGGCCGACCAGCCGCGCCCGGTAGCTCCCTGGGCTCTGCCCGGT‑CCACTTCTTGAACGCCCGGTGGAAGGCACTCGGTTCCTGAAACCCCGCCTGCTCGGCAATTTCGGCAATg                                                      >  8:70510/1‑149 (MQ=255)
                                                                                                  aTGCCCGCTCCTACAGGTAGAACGCAGGCCGACCAGCCGCGCCCGGTAGCTCCCTGGGCTCTGCCCGGT‑CCACTTCTTGAACGCCCGGTGGAAGGCACTCGGTTCCTGAAACCCCGCCTGCTCGGCAATTTCGGCAATGCTCAACgcgc                                            >  3:96781/1‑149 (MQ=255)
                                                                                                      ccGCTCCTACAGGTAGAACGCAGGCCGACCAGCCGCGCCCGATAGCTCCCTGGGCTCTGCCCGGT‑CCACTTCTTGAACGCCCGGTGGAAGGCACTCGGTTCCTGAAACCCCGCCTGCTCGGCAATTTCGGCAATGCTCAACGCGCCTTc                                        <  1:274078/149‑1 (MQ=255)
                                                                                                      ccGCTCCTACAGGTAGAACGCAGGCCGACCAGCCGCGCCCGATAGCTCCCTGGCCTCTGCCCGGT‑CCACTTCTTGAACGCCCGGTGGAAGGCACTCGGTTCCTGAAACCCCGCCTGCTCGGCAATTTCGGCAATGCTCAACGCGCCTTc                                        <  1:278148/149‑1 (MQ=255)
                                                                                                              aCAGGTAGAACGCAGGCCGACCAGCCGCGCCCGGTAGCTCCCTGGGCTCTGCCCGGT‑CCACTTCTTGAACGCCCGGTGGAAGGCACTCGGTTCCTGAAACCCCGCCTGCTCGGCAATTTCGGCAATGCTCAACGCGCCTTCGCGCAGcc                                >  2:270520/1‑149 (MQ=255)
                                                                                                               cAGGTAGAACGCAGGCCGACCAGCCGCGCCCGGTAGCTCCCTGGGCTCTGCCCGGT‑CCACTTCTTGAACGCCCGGTGGAAGGCACTCGGTTCCTGAAACCCCGCCTGCTCGGCAATTTCGGCAATGCTCAACGCGCCTTCGCGCAGc                                 <  4:96781/147‑1 (MQ=255)
                                                                                                                aGGTAGAACGCAGGCCGACCAGCCGCGCCCGGTAGCTCCCTGGGCTCTGCCCGGT‑CCACTTCTTGAACGCCCGGTGGAAGGCACTCGGTTCCTGAAACCCCGCCTGCTCGGCAATTTCGGCAATGCTCAACGCGCCTTCGCGCAGCCGc                              >  5:222946/1‑149 (MQ=255)
                                                                                                                  gTAGAACGCAGGCCGACCAGCCGCGCCCGGTAGCTCCCTGGGCTCTGCCCGGTATCACTTCTTGAACGCCCGGTGGAAGGCACTCGGTTCCTGAAACCCCGCCTGCTCGGCAATTTCGGCAATGCTCAACGCGCCTTCGCGCAGCCGCt                             >  1:259588/1‑149 (MQ=255)
                                                                                                                      aaCGCAGGCCGACCAGCCGCGCCCGGTAGCTCCCTGGGCTCTGCCCGGT‑CCACTTCTTGAACGCCCGGTGGAAGGCACTCGGTTCCTGAAACCCCGCCTGCTCGGCAATTTCGGCAATGCTCAACGCGCCTTCGCGCAGCCGCTCGAAg                        <  3:312495/149‑1 (MQ=255)
                                                                                                                        cGCAGGCCGACCAGCCGCGCCCGGTAGCTCCCTGGGCTCTGCCCGGT‑CCACTTCTTGAACGCCCGGTGGAAGGCACTCGGTTCCTGAAACCCCGCCTGCTCGGCAATTTCGGCAATGCTCAACGCGCCTTCGCGCAGCCGCTCGAAGGc                      <  8:9364/149‑1 (MQ=255)
                                                                                                                           aGGCCGACCAGCCGCGCCCGGTAGCTCCCTGGGCTCTGCCCGGT‑CCACTTCTTGAACGCCCGGTGGAAGGCACTCGGTTCCTGAAACCCCGCCTGCTCGGCAATTTCGGCAATGCTCAACGCGCCTTCGAGCGGCTGCGCGAAGGcgcg                   <  8:311823/149‑4 (MQ=255)
                                                                                                                           aGGCCGACCAGCCGCGCCCGGTAGCTCCCTGGGCTCTGCCCGGT‑CCACTTCTTGAACGCCCGGTGGAAGGCACTCGGTTCCTGAAACCCCGCCTGCTCGGCAATTTCGGCAATGATCAACGCGCCTTCGCGCCGCCGCTCGAAGGCCAt                   >  8:313918/1‑149 (MQ=255)
                                                                                                                            ggCCGACCAGCCGCGCCCGGTAGCTCCCTGGGCTCTGCCCGGT‑CCACTTCTTGAACGCCCGGTGGAAGGCACTCGGTTCCTGAAACCCCGCCTGCTCGGCAATTTCGGCAATGCTCAACGCGCCTTCGCGCAGCCGCTCGAAGGCCATg                  <  2:93103/149‑1 (MQ=255)
                                                                                                                             gCCGACCAGCCGCGCCCGGTAGCTCCCTGGGCTCTGCCCGGT‑CCACTTCTTAAACGCCCGGTGGAAGGCACTCGGTTCCTGAAACCCCGCCTGCTCGGCAATTTCGGCAATGCTCAACGCGCCTTCGCGCAGCCGCTCGAAGGCCATgg                 >  1:253860/1‑149 (MQ=255)
                                                                                                                                   cAGCCGCGCCCGGTAGCTCCCTGGGCTCTGCCCGGT‑CCACTTCTTGAACGCCCGGTGGAAGGCACTCGGTTCCTGAACACCCGCCTGCTCGGCAATTTCGGCAATGCTCAACGCGCCTTCGCACAGCCGCTCGAAGGCCATGGCCCGgc           >  7:218222/1‑149 (MQ=255)
                                                                                                                                      ccGCGCCCGGTAGCTCCCTGGGCTCTGCCCGGT‑CCACTTCTTGAACGCCCGGTGGAAGGCACTCGGTTCCTGAAACCCCGCCTGCTCGGCAATTTCGGCAATGCTCAACGCGCCTTCGCGCAGCCGCTCGAAGGCCATGGCCCg             <  6:134194/144‑1 (MQ=255)
                                                                                                                                      ccGCGCCCGGTAGCTCCCTGGGCTCTGCCCGGT‑CCACTTCTTGAACGCCCGGTGGAAGGCACTCGGTTCCTGAAACCCCGCCTGCTCGGCAATTTCGGCAATGCTCAACGCGCCTTCGCGCAGCCGCTCGAAGGCCATGGCCCg             >  5:134194/1‑144 (MQ=255)
                                                                                                                                      ccGCGCCCGGTAGCTCCCTGGGCTCTGCCCGGT‑CCACTTCTTGAACGCCCGGTGGAAGGCACTCGGTTCCTGAAACCCCGCCTGCTCGGCAATTTCGGCAATGCTCAACGCGCCTTCGCGCAGCCGCTCGAAGGCCATGGCCCGGCGCa        >  2:236994/1‑149 (MQ=255)
                                                                                                                                        gcgcCCGGTAGCTCCCTGGGCTCTGCCCGGT‑CCACTTCTTGAACGCCCGGTGGAAGGCACTCGGTTCCTGAAACCCCGCCTGCTCGGCAATTTCGGCAATGCTCAACGCGCCTTCGCGCAGCCGCTCGAAGGCCATGGCCCGGCGCACt      <  7:70510/149‑1 (MQ=255)
                                                                                                                                            ccGGTAGCTCCCTGGGCTCTGCCCGGT‑CCACTTCTTGAACGCCCGGTGGAAGGCACTCGGTTCCTGAAACCCCGCCTGCTCGGCAATTTCGGCAATGCTCAACGCGCCTTCGCGCAGCCGCTCGAAGGCCATGGCCCGGCGCACTTCAt  >  2:65537/1‑149 (MQ=255)
                                                                                                                                               |                                                                                                                                                  
CCCGCTCCTACAATTCTGGGCGTGCCCTGTAGGAGCGGGTTTACCCGCGAACACCGGCAGAGCCGGTGCCATCCACCGCGGTGGTTT‑CTTCGCGGGCATGCCCGCTCCTACAGGTAGAACGCAGGCCGACCAGCCGCGCCCGGTAGCTCCCTGGGCTCTGCCCGGT‑CCACTTCTTGAACGCCCGGTGGAAGGCACTCGGTTCCTGAAACCCCGCCTGCTCGGCAATTTCGGCAATGCTCAACGCGCCTTCGCGCAGCCGCTCGAAGGCCATGGCCCGGCGCACTTCAT  >  NC_002947/4282101‑4282388

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑