Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,296,175 A→G 12.2% intergenic (+94/‑131) PP_3765 → / → gloA H‑NS family protein MvaT/lactoylglutathione lyase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,296,1750AG12.2% 100.8 / 6.3 41intergenic (+94/‑131)PP_3765/gloAH‑NS family protein MvaT/lactoylglutathione lyase
Reads supporting (aligned to +/- strand):  ref base A (17/19);  new base G (2/3);  total (19/22)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.93e-01

GAAAGAACAGTATGGGGCCGCCACGGTAGAAAGCTGGGTACGCTGATGAAATGCCCACATAGGCTTCACACTTTTTTCACAAGGACTGGCTCAGTATCGAAACAGCTAAAGGACTAGCGACCCCATCACGCGCCCGCACATGCGGGCCTCTAATTTCAGCGCCCTGCCTTTGCAGGGCGCTTTCATTTGCGCAATCGGCGCACTGCCGTATCATGGCCAGCCTGTCTGTTTTGCCGGCGCCCTGCGCCCGGCCCCGTCTCAGGAGTTCCCATGAGCCTGCACGA  >  NC_002947/4296036‑4296319
                                                                                                                                           |                                                                                                                                                
gAAAGAACAGTATGGGGCCGCCACGGTAGAAAGCTGGGTACGCTGATGAAATGCCCACATAGGCTTCACACTTTTTTCACAAGGACTGGCTCAGTATCGAAACAGCTAAAGGACTAGCGACCCCATCACGCGCCCGCACATGCGGGc                                                                                                                                           <  1:243977/147‑1 (MQ=255)
gAAAGAACAGTATGCGGCCGCCACGGTAGAAAGCTGGGTACGCTGATGAAATGCCCACATAGGCTTCACACTTTTTTCACAAGGACTGGCTCAGTATCGAAACAGCTAAAGGACTAGCGACCCCATCACGCGCCCGCATGTGCGGGcg                                                                                                                                          <  1:292605/148‑2 (MQ=255)
  aaGAACAGTATGGGGCCGCCACGGTAGAAAGCTGGGTACGCTGATGAAATGCCCACATAGGCTTCACACTTTTTTCACAAGGACTGGCTCAGTATCGAAACAGCTAAAGGACTAGCGACCCCATCACGCGCCCGCACATGCGGGCctct                                                                                                                                       <  6:347992/149‑1 (MQ=255)
    gAACAGTATGGGGCCGCCACGGTAGAAAGCTGGGTACGCTGATGAAATGCCCACATAGGCTTCACACTTTTTTCACAAGGACTGGCTCAGTATCGAAACAGCTAAAGGACTAGCGACCCCATCACGCGCCCGCACATGCGGGCCTCTaa                                                                                                                                     <  2:262202/149‑1 (MQ=255)
            tGGGGCCGCCACGGTAGAAAGCTGGGTACGCTGATGAAATGCCCACATAGGCTTCACACTTTTTTCACAAGGACTGGCTCAGTATCGAAACAGCTAAAGGACTAGCGACCCCATCACGCGCCCGCACATGCGGGCCTCTAATTTCAgc                                                                                                                              <  2:151260/148‑1 (MQ=255)
                   gccACGGTAGAAAGCTGGGTACGCTGATGAAATGCCCACATAGGCTTCACACTTTTTTCATAAGGACTGGCTCAGTATCGAAACAGCTAAAGGACTAGCGACCCCATCACGCGCCCGCACATGCGGGCCTCTAATTTCAGCGCCCTGcc                                                                                                                      <  4:288916/149‑1 (MQ=255)
                         gTAGAAAGCTGGGTACGCTGATGAAATGCCCACATAGGCTTCATATTTTTTTCACAAGGACTGGCTCAGTATCGAAACAGCTAAAGGACTAGCGACCCCATCACGCGCCCGCACATGCGGGCCTCTAATTTCAGCGCCCTGCCTTTGCa                                                                                                                <  1:283142/149‑1 (MQ=255)
                                         gCTGATGAAATGCCCACATAGGCTTCACACTTTTTTCACAAGGACTGGCTCAGTATCGAAACAGCTAAAGGACTAGCGACCCCATCACGCGCCCGCACATGCGGGCCTCTAATTTCa                                                                                                                                <  8:248165/117‑1 (MQ=255)
                                         gCTGATGAAATGCCCACATAGGCTTCACACTTTTTTCACAAGGACTGGCTCAGTATCGAAACAGCTAAAGGACTAGCGACCCCATCACGCGCCCGCACATGCGGGCCTCTAATTTCa                                                                                                                                >  7:248165/1‑117 (MQ=255)
                                                      ccACATAGGCTTCACACTTTTTTCACAAGGACTGGCTCAGTATCGAAACAGCTAAAGGACTAGCGACCCCATCACGCGCCCGCACATGCGGGCCTCTAATTTCAGCGCCCTGCCTTTGCAGGGCGCTTTCAttt                                                                                                  <  6:240576/134‑1 (MQ=255)
                                                      ccACATAGGCTTCACACTTTTTTCACAAGGACTGGCTCAGTATCGAAACAGCTAAAGGACTAGCGACCCCATCACGCGCCCGCACATGCGGGCCTCTAATTTCAGCGCCCTGCCTTTGCAGGGCGCTTTCAttt                                                                                                  >  5:240576/1‑134 (MQ=255)
                                                                ttCACACTTTTTTCACAAGGACTGGCTCAGTATCGAAACAGCTAAAGGACTAGCGACCCCATCACGCGCCCGCACATGCGGGCCTCTAATTTCAGCGCCCTGCCTTTGCAGGGCGCTTTCATTTGCGCAATCGGCGCACTGCCGt                                                                             >  1:64246/1‑145 (MQ=255)
                                                                ttCACACTTTTTTCACAAGGACTGGCTCAGTATCGAAACAGCTAAAGGACTAGCGACCCCATCACGCGCCCGCACATGCGGGCCTCTAATTTCAGCGCCCTGCCTTTGCAGGGCGCTTTCATTTGCGCAATCGGCGCACTGCCGt                                                                             <  2:64246/145‑1 (MQ=255)
                                                                       ttttttCACAAGGACTGGCTCAGTATCGAAACAGCTAAAGGACTAGCGACCCCATCACGCGCCCGCACATGCGGGCCTCTAATTTCAGCGCCCTGCCTTTGCAGGGCGCTTTCATTTGCGCAATCGGCGCACTGCCGTATCATGGCCAg                                                                  >  8:203453/1‑149 (MQ=255)
                                                                         ttttCACAAGGACTGGCTCAGTATCGAAACAGCTAAAGGACTAGCGACCCCATCACGCGCCCGCACATGCGGGCCTCTAATTTCAGCGCCCTGCCTTTGCAGGGCGCTTTCATTTGCGCAATCGGCGCACTGCCGTATCATGGCCAg                                                                  <  8:301869/147‑1 (MQ=255)
                                                                             cacaAGGACTGGCTCAGTATCGAAACAGCTAAAGGACTAGCGACCCCATCACGCGCCCGCACATGCGGGCCTCTAATTTCAGCGCCCTGCCTTTGCAGGGCGCTTTCATTTGCGCAATCGGCGCACTGCCGTATCATGGCCAGCctgt                                                             <  3:66723/148‑1 (MQ=255)
                                                                              acaAGGACTGGCTCAGTATCGAAACAGCTAAAGGACTAGCGACCCCATCACGCGCCCGCACATGCGGGCCTCTAATTTCAGCGCCCTGCCTTTGCAGGGCGCTTTCATTTGCGCAATCGGCGCACTGCCGTATCATGGc                                                                     <  5:283948/139‑1 (MQ=255)
                                                                              acaAGGACTGGCTCAGTATCGAAACAGCTAAAGGACTAGCGACCCCATCACGCGCCCGCACATGCGGGCCTCTAATTTCAGCGCCCTGCCTTTGCAGGGCGCTTTCATTTGCGCAATCGGCGCACTGCCGTATCATGGc                                                                     >  6:283948/1‑139 (MQ=255)
                                                                                        gCTCAGTATCGAAACAGCTAAAGGACTAGCGACCCCATCACGCGCCCGCACATGCGGGCCTCTAATTTCAGCGCCCTGCCTTTGCAGGGCGCTTTCATTTGCGCAATCGGCGCACTGCCGTATCATGGCCAGCCTGTCTGTTTTGCCgg                                                 >  1:337718/1‑149 (MQ=255)
                                                                                          tCAGTATCGAAACAGCTAAAGGACTAGCGACCCCATCACGCGCCCGCACATGCGGGCCTCTAATTTCAGCGCCCTGCCTTTGCAGGGCGCTTTCATTTGCGCAATCGGCGCACTGCCGTATCATgg                                                                      >  8:168094/1‑126 (MQ=255)
                                                                                                tCGAAACAGCTAAAGGACTAGCGACCCCATCACGCGCCCGCACATGCGGGCCTCTAATTTCAGCGCCCTGCC‑TTGCAGGGCGCTTTCATTTGCGCAATCGGCGCACTGCCGTATCATGGCCAGCCTGTCTGTTTTGc                                                    >  4:153767/1‑137 (MQ=255)
                                                                                                tCGAAACAGCTAAAGGACTAGCGACCCCATCACGCGCCCGCACATGCGGGCCTCTAATTTCAGCGCCCTGCC‑TTGCAGGGCGCTTTCATTTGCGCAATCGGCGCACTGCCGTATCATGGCCAGCCTGTCTGTTTTGc                                                    <  3:153767/137‑1 (MQ=255)
                                                                                                        gCTAAAGGACTAGCGACCCCATCACGCGCCCGCACATGCGGGCCTCTAATTTCAGCGCCCTGCCTTTGCAGGGCGCTTTCATTTGCGCAATCGGCGCACTGCCGTATCATGGCCAGCCTGTCTGTTTTGCCGGCGCCCTGCGCCCGGcc                                 >  2:131445/1‑149 (MQ=255)
                                                                                                          tAAAGGACTAGCGACCCCATCACGCGCCCGCACATGCGGGCCTCTAATTTCAGCGCCCTGCCTTTGCAGGGCGCTTTCATTTGCGCAATCGGCGCACTGCCGTATCATGGCCAGCCTGTCTGTTTTGCCGGCGCCCTGCGCCCGGcccc                               >  5:194677/1‑149 (MQ=255)
                                                                                                            aaGGACTAGCGACCCCATCACGCGCCCGCACATGCGGGCCTCTAATTTCAGCGCCCTGCCTTTGCAGGGCGCTTTCATTTGCGCAATCGGCGCACTGCCGTATCATGGCCAGCCTGTCTGTTTTGCCGGCGCCCTGCGCCCGGcccc                               <  3:24302/147‑1 (MQ=255)
                                                                                                            aaGGACTAGCGACCCCATCACGCGCCCGCACATGCGGGCCTCTAATTTCAGCGCCCTGCCTTTGCAGGGCGCTTTCATTTGCGCAATCGGCGCACTGCCGTATCATGGCCAGCCTGTCTGTTTTGCCGGCGCCCTGCGCCCGGCCCCGt                             >  3:64974/1‑149 (MQ=255)
                                                                                                               gACTAGCGACCCCATCACGCGCCCGCACATGCGGGCCTCTAATTTCAGCGCCCTGCCTTTGCAGGGCGCTTTCATTTGCGCAATCGGCGCACTGCCGTATCATGGCCAGCCTGTCTGTTTTGCCGGCGCCCTGCGCCCGGCCCCGtct                           >  5:237923/1‑148 (MQ=255)
                                                                                                                 cTAGCGACCCCATCACGCGCCCGCACATGCGGGCCTCTAATTTCAGCGCCCTGCCTTTGCAGGGCGCTTTCATTTGCGCAATCGGCGCACTGCCGTATCATGGCCAGCCTGTCTGTTTTGCCGGCGCCCTGCGCCCGGCCCCGTCTCAg                        <  3:76448/149‑1 (MQ=255)
                                                                                                                 cTAGCGACCCCATCACGCGCCCGCACATGCGGGCCTCTAATTTCAGCGCCCTGCCTTTGCAGGGCGCTTTCATTTGCGCAATCGGCGCACTGCCGTATCATGGCCAGCCTGTCTGTTTTGCCGGCGCCCTGCGCCCGGCCCCGTCTCAg                        <  4:124215/149‑1 (MQ=255)
                                                                                                                 cTAGCGACCCCATCACGCGCCCGCACATGCGGGCCTCTAATTTCAGCGCCCTGCCTTTGCAGGGCGCTTTCATTTGCGCAATCGGCGCACTGCCGTATCATGGCCAGCCTGTCTGTTTTGCCGGCGCCCTGCGCCCGGCCCCGTCTCAg                        >  3:124215/1‑149 (MQ=255)
                                                                                                                    gCGACCCCATCACGCGCCCGCACATGCGGGCCTCTAATTTCAGCGCCCTGCCTTTGCAGGGCGCTTTCATTTGCGCAATCGGCGCACTGCCGTATCATGGCCAGCCTGTCTGTTTTGCCGGCGCCCTGCGCCCGGCCCCGTCTCAg                        >  4:76448/1‑146 (MQ=255)
                                                                                                                    gCGACCCCATCACGCGCCCGCACATGCGGGCCTCTAATTTCAGCGCCCTGCCTTTGCAGGGCGCTTTCATTTGCGCAATCGGCGCACTGCCGTATCATGGCCAGCCTGTCTGTTTTGCCGGCGCCCTGCGCCCGGCCCCGTCTCAGGa                      <  7:203453/148‑1 (MQ=255)
                                                                                                                             ttagaggcCCGCATGTGCGGGCCTCTAATTTCAGCGCCCTGCCTTTGCAGGGCGCTTTCATTTGCGCAATCGGCGCACTGCCGTATCATGGCCAGCCTGTCTGTTTTGCCGGCGCCCTGCGCCCGGCCCCGTCTCAGGAGTTCCCAt              >  8:54963/7‑147 (MQ=255)
                                                                                                                              cACGCGCCCGCACATGCGGGCCTCTAATTTCAGCGCCCTGCCTTTGCAGGGCGCTTTCATTTGCGCAATCGGCGCACTGCCGTATCATGGCCAGCCTGTCTGTTTTGCCGGCGCCCTGCGCCCGGCCCCGTCTCAg                        <  2:330193/136‑1 (MQ=255)
                                                                                                                              cACGCGCCCGCACATGCGGGCCTCTAATTTCAGCGCCCTGCCTTTGCAGGGCGCTTTCATTTGCGCAATCGGCGCACTGCCGTATCATGGCCAGCCTGTCTGTTTTGCCGGCGCCCTGCGCCCGGCCCCGTCTCAg                        >  1:330193/1‑136 (MQ=255)
                                                                                                                                 gcgcCCGCACATGCGGGCCTCTAATTTCAGCGCCCTGCCTTTGCAGGGCGCTTTCATTTGCGCAATCGGCGCACTGCCGTATCATGGCCAGCCTGTCTGTTTTGCCGGCGCCCTGCGCCCGGCCCCGTCTCAGGAGTTCCCATGAGCCt        >  6:213344/1‑149 (MQ=255)
                                                                                                                                 aggcCCGCATGTGCGGGCCTCTAATTTCAGCGCCCTGCCTTTGCAGGGCGCTTTCATTTGCGCAATCGGCGCACTGCCGTATCATGGCCAGCCTGTCTGTTTTGCCGGCGCCCTGCGCCCGGCCCCGTCTCAGGAGTTCCCATGa            >  6:183450/3‑145 (MQ=255)
                                                                                                                                 aggcCCGCATGTGCGGGCCTCTAATTTCAGCGCCCTGCCTTTGCAGGGCGCTTTCATTTGCGCAATCGGCGCACTGCCGTATCATGGCCAGCCTGTCTGTTTTGCCGGCGCCCTGCGCCCGGCCCCGTCTCAGGAGTTCCCATGa            <  5:183450/143‑1 (MQ=255)
                                                                                                                                    cccGCATGTGCGGGCCTCTAATTTCAGCGCCCTGCCTTTGCAGGGCGCTTTCATTTGCGCAATCGGCGCACTGCCGTATCATGGCCAGCCTGTCTGTTTTGCCGGCGCCCTGCGCCCGGCCCCGTCTCAGGAGTTCCCATGAGCCTGCa     <  7:54963/149‑1 (MQ=255)
                                                                                                                                    cccGCACATGCGGGCCTCTAATTTCAGCGCCCTGCCTTTGCAGGGCGCTTTCATTTGCGCAATCGGCGCACTGCCGTATCATGGCCAGCCTGTCTGTTTTGCCGGCGCCCTGCGCCCGGCCCCGTCTCAGGAGTTCCCATGAGCCTGCa     <  5:213344/149‑1 (MQ=255)
                                                                                                                                       gCACATGCGGGCCTCTAATTTCAGCGCCCTGCCTTTGCAGGGCGCTTTCATTTGCGCAATCGGCGCACTGCCGTATCATGGCCAGCCTGTCTGTTTTGCCGGCGCCCTGCGCCCGGCCCCGTCTCAGGAGTTCCCATGAGCCTGCACGa  >  7:181058/1‑149 (MQ=255)
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GAAAGAACAGTATGGGGCCGCCACGGTAGAAAGCTGGGTACGCTGATGAAATGCCCACATAGGCTTCACACTTTTTTCACAAGGACTGGCTCAGTATCGAAACAGCTAAAGGACTAGCGACCCCATCACGCGCCCGCACATGCGGGCCTCTAATTTCAGCGCCCTGCCTTTGCAGGGCGCTTTCATTTGCGCAATCGGCGCACTGCCGTATCATGGCCAGCCTGTCTGTTTTGCCGGCGCCCTGCGCCCGGCCCCGTCTCAGGAGTTCCCATGAGCCTGCACGA  >  NC_002947/4296036‑4296319

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑