| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 4,344,319 | A→G | 22.6% | intergenic (+50/‑106) | PP_3817 → / → PP_3818 | polyamine ABC transporter ATP‑binding protein/phosphate‑binding protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 4,344,319 | 0 | A | G | 22.6% | 49.9 / 13.1 | 32 | intergenic (+50/‑106) | PP_3817/PP_3818 | polyamine ABC transporter ATP‑binding protein/phosphate‑binding protein |
| Reads supporting (aligned to +/- strand): ref base A (14/10); new base G (4/3); total (18/14) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.52e-01 | |||||||||||
GACAGCGTGAAGGTGCGTTGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCTGCCAGATCGAGCACCGCCCGCGCGGCACACGATCTGGCAGGCGCTGCACTATTGGAGGCGTACGCCCCGTCACATTATCATCACGCCACTGTCGTAATCTG‑CCGGCCAACTCTATGCCACAGGTCACCCGGCATGCTCCGCCTGCTTGTCTTGCTGCTCACCCTGCTACCACTCG > NC_002947/4344177‑4344467 | gACAGCGTGAAGGTGCGTTGGGCAGTAGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACG‑TGCAGCGCCTGCCAGATCGTGTGCCGCGCGGGCGGTGCTCGATc < 5:182463/149‑1 (MQ=37) aGCGTGAAGGTGCGTTGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCTGCCAGATCGTGTGCCGCGCGGGCGGTGCTCGATCTg < 3:276146/149‑1 (MQ=37) tGAAGGTGCGTTGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCTGCCAGATCGAGCACCGCCCGCGCGGCACACGATCTGGCAg < 1:9396/149‑1 (MQ=255) gTGCGTTGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCTGCCAGATCGAGCACCGCCCGCGCGGCACACGATCTGGCAGGCGCt > 3:282843/1‑149 (MQ=255) gggCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCTGCCAGATCGAGCACCGCCCGCGCGGCACACGATCTGGCAGGCGCTGCACTAt > 3:105250/1‑149 (MQ=255) ttGCATTTATACCCAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGAATTAACGCTCTTGTCCGTTGCCGCGCCGGCCAGATCGACCACCGCCCGCGCGGCACACTATCTGGCAGGCGCTGCACTATTGGAGGCGTACGcccc < 2:132443/149‑1 (MQ=255) tGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCTGCCAGATCGAGCACCGCCCGCGCGGCACACGATCTGGCAGGCGCTGCACTATTGGAGGCGTACGCCCCg > 3:52606/1‑149 (MQ=255) tttATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCTGCCAGATCGTGTGCCGCGCGGGCGGTGCTCGATCTGGCAGGCGCt < 8:176292/121‑1 (MQ=21) tttATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCTGCCAGATCGTGTGCCGCGCGGGCGGTGCTCGATCTGGCAGGCGCt > 7:176292/1‑121 (MQ=21) ccGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCTGCCAGATCGAGCACCGCCCGCGCGGCACACGATCTGGCAGGCGCTGCACTATTGGAGGCGTACGCCCCGTCACATTATCATCACGCCa > 8:95252/1‑149 (MQ=255) aCCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCTGCCAGATCGAGCACCGCCCGCGCGGCACACGATCTGGCAGGCGCTGCACTATTGGAGGCGTACGCCCCGTCACATTATCATCACGCCACTGTCGTaa > 2:243060/1‑149 (MQ=255) cTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCTGCCAGATCGAGCACCGCCCGCGCGGCACACGATCTGGCAGGCGCTGCACTATTGGAGGCGTACGCCCCGTCACATTATCATCACGCCACTGTCGTAATc < 7:95252/149‑1 (MQ=255) gcgcATTAACGCTCTTGTACGTTGCTCCGCGTGCCAGATTGAGCACCGCCCGCGGGGCTCACGTTGTGGCTGGCGCTGCACTATTGGAGGCGTACGCCCCGTCACATTATCATCACGCCACTGTCGTAATCTG‑CCGGCCAACTCTATGc < 2:287008/149‑1 (MQ=255) aaCGCTCTTGTACGTTGCAGCGCCTGCCAGATCGAGCACCGCCCGCGCGGCACACGATCTGGCAGGCGCTGCACTATTGGAGGCGTACGCCCCGTCACATTATCATCAAGCCACTGTCGTAATCTG‑CCGGCCAACTCTATGCCACAggg > 1:313391/1‑148 (MQ=255) ttGTACGTTGCAGCGCCTGCCAGATCGAGCACCGCCCGCGCGGCACACGATCTGGCAGGCGCTGCACTATTGGAGGCGTACGCCCCGTCACATTATCATCACGCCACTGTCGTAATCTG‑CCGGCCAACTCTATGCCACAGGTCACCCgg > 3:23888/1‑149 (MQ=255) gTACGTTGCAGCGCCTGCCAGATCGAGCACCGCCCGCGCGGCACACGATCTGGCAGGCGCTGCACTATTGGAGGCGTACGCCCCGTCACATTATCATCACGCCACTGTCGTAATCTG‑CCGGCCAACTCTATGCCACAGGTCACCCGGc < 1:243060/148‑1 (MQ=255) aCGTTGCAGCGCCTGCCATATCGAGCACCGCCCGCGCGGCACACGATCTGGCAGGCGCTGCACTATTGGAGGCGTACGCCCCGTCACATTATCATCACGCCACTGTCGTAATCTG‑CCGGCCAACTCTATGCCACAGGTCACCCGGCAt < 4:282843/148‑1 (MQ=255) cAGCGCCTGCCAGATCGAGCACCGCCCGCGCGGCACACGATCTGGCAGGCGCTGCACTATTGGAGGCGTACGCCCCGTCACATTATCATCACGCCACTGTCGTAATAGGACCGGCCAACACTATGCCACAGGTCCCCCGGCATGCTcgg > 1:283626/1‑147 (MQ=255) cTGCCAGATCGTGTGCCGCGCGGGCGGTGCTCGATCTGGCAGGCGCTGCACTATTGGAGGCGTACGCCCCGTCACATTATCATCACGCCACTGTCGTAATCTG‑CCGGCCAACTCTATGCCACAGGTCACCCGGCATGCTCCGCCTGCtt > 6:49814/1‑149 (MQ=37) ccAGATCGTGTGCCGCGCGGGCGGTGCTCGATCTGGCAGGCGCTGCCCTATTGGAGGCGTACGCCCCGTCACATTATCATCACGCCACTGTCGTAATCTG‑CCGGCCAACTCTATGCCACAGGTCACCCGGCATGCTCCGCCTGCTTGTc > 5:75351/1‑149 (MQ=37) gATCGAGCACCGCCCGCGCGGCACACGATCTGGCAGGCGCTGCACTATTGGAGGCGTACGCCCCGTCACATTATCATCACGCCACTGTCGTAATCTG‑CCGGCCAACTCTATGCCACAGGTCACCCGGCATGCTCCGCCTGCTTGTCTtg < 1:138900/149‑1 (MQ=255) gATCGAGCACCGCCCGCGCGGCACACGATCTGGCAGGCGCTGCACTATTGGAGGCGTACGCCCCGTCACATTATCATCACGCCACTGTCGTAATCTG‑CCGGCCAACTCTATGCCACAGGTCACCCGGCATGCTCCGCCTGCTTGTCTtg > 2:138900/1‑149 (MQ=255) gATCGAGCACCGCCCGCGCGGCACACGATCTGGCAGGCGCTGCACTATTGGAGGCGTACGCCCCGTCACATTATCATCACGCCACTGTCGTAATCTG‑CCGGCCAACTCTATGCCACAGGTCACCCGGCATGCTCCGCCTGCTTGTCTtg > 2:77710/1‑149 (MQ=255) tCGAGCACCGCCCGCGCGGCACACGATCTGGCAGGCGCTGCACTATTGGAGGCGTACGCCCCGTCACATTATCATCACGCCACTGTCGTAATCTG‑CCGGCCAACTCTATGCCACAGGTCACCCGGCATGCTCCGCCTGCTTGTCTtgct > 8:239425/1‑149 (MQ=255) gagaACCGCCCGCGCGGCACACGATCTGGCAGGCGCTGCACTATTGGAGGCGTACGCCCCGTCACATTATCATCACGCCACTGTCGTAATCTG‑CCGGCCCACTCTATGCCACAGGTCACCCGGCATGCTCCGCCTGCTTGTCTtgca > 3:260696/1‑146 (MQ=255) gAGCACCGCCCGCGCGGCACACGATCTGGCAGGCGCTGCACTATTGGAGGCGTACGCCCCGTCACATTATCATCACGCCACTGTCGTAATCTG‑CCGTCCAACTCTATGCCACAGGTCACCCGGCATGCTCCGCCTGCTTGTCTtg < 4:260696/145‑1 (MQ=255) gAGCACCGCCCGCGCGGCACACGATCTGGCAGGCGCTGCACTATTGGAGGCGTACGCCCCGTCACATTATCATCACGCCACTGTCGTAATCTG‑CCGGCCAACTCTATGCCACAGGTCACCCGGCATGCTCCGCCTGCTTGTCTtgctg < 4:23888/148‑1 (MQ=255) tgccgcgcggGCGGTGCTCGATCTGGCAGGCGCTGCACTATTGGAGGCGTACGCCCCGTCACATTATCATCACGCCACTGTCGTAATCTG‑CCGGCCAACTCTATGCCACAGGTCACCCGGCATGCTCCGCCTGCTTGTCTTGCTGCTCa > 8:278248/3‑149 (MQ=39) cACCGCCCGCGCGGCACACGATCTGGCAGGCGCTGCACTATTGGAGGCGTACGCCCCGTCACATTATCATCACGCCACTGTCGTAATCTG‑CCGGCCAACTCTATGCCACAGGTCACCCGGCATGCTCCGCCTGCTTGTCTTGCTGCTCa < 4:105250/149‑1 (MQ=255) cACCGCCCGCGCGGCACACGATCTGGCAGGCGCTGCACTATTGGAGGCGTACGCCCCGTCACATTATCATCACGCCACTGTCGTAATCTG‑CCGGCCAACTCTATGCCACAGGTCACCCGGCATGCTCCGCCTGCTTGTCTTGCTGCTCa > 8:137793/1‑149 (MQ=255) cccgcGCGGCACACGATCTGGCAGGCGCTGCACTATTGGAGGCGTACGCCCCGTCACATTATCATCACGCCACTGTCGTAATCTG‑CCGGCCAACTCTATGCCACAGGTCACCCGGCATGCTCCGCCTGCTTGTCTTGCTGCTCACCCTg < 1:77710/149‑1 (MQ=255) ggCACACGATCTGGCAGGCGCTGCACTATTGGAGGCGTACGCCCCGTCACATTATCATCACGCCACTGTCGTAATCTG‑CCGGCCAACTCTATGCCACAGGTCACCCGGCATGCTCCGCCTGCTTGTCTTGCTGCTCACCCTGCTACCAc > 1:304441/1‑149 (MQ=255) acacGATCTGGCAGGCGCTGCACTATTGGAGGCGTACGCCCCGTCACATTATCATCACGCCACTGTCGTAATCTG‑CCGGCCAACTCTATGCCACAGGTCACCCGGCATGCTCCGCCTGCTTGTCTTGCTGCTCACCCTGCTACCACTcg > 4:173205/1‑149 (MQ=255) acacGATCTGGCAGGCGCTGCACTATTGGAGGCGTACGCCCCGTCACATTATCATCACGCCACTGTCGTAATCTG‑CCGGCCAACTCTATGCCACAGGTCACCCGGCATGCTCCGCCTGCTTGTCTTGCTGCTCACCCTGCTACCACTcg > 1:221833/1‑149 (MQ=255) | GACAGCGTGAAGGTGCGTTGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCTGCCAGATCGAGCACCGCCCGCGCGGCACACGATCTGGCAGGCGCTGCACTATTGGAGGCGTACGCCCCGTCACATTATCATCACGCCACTGTCGTAATCTG‑CCGGCCAACTCTATGCCACAGGTCACCCGGCATGCTCCGCCTGCTTGTCTTGCTGCTCACCCTGCTACCACTCG > NC_002947/4344177‑4344467 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |